CmoCh16G010230 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh16G010230 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 1.1e-251
Identity = 432/765 (56.47%), Postives = 553/765 (72.29%), Query Frame = 0

Query: 11  LPFFLFALLQTSTIAAQK---SYIVYLGSHSHGLNPSALDLQLVTQTHYNLLGSVLGSNE 70
           L   L   + +  I A K   SY+VY G+HSH    +   +  V +THY+ LGS  GS E
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 71  AAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWNFLGVEND 130
            A ++IFYSY K+INGFAA L+H +A +I+KHP+V+SV+ NK LKLHTTRSW+FLG+E++
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 131 GGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEG--GSNFRC 190
             +PS S+W  +RFGE TII N+DTGVWPESKSF DEG GPIP+RWKG C+    + F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189

Query: 191 NRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIFGNGYG 250
           NRKLIGARYFNKGY ++V    LNSS+++ RD DGHG+HTLSTA G+FVPGVSIFG G G
Sbjct: 190 NRKLIGARYFNKGYAAAV--GHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 249

Query: 251 TAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAFEFSED 310
           TAKGGSP A VAAYKVCWPP    EC+ ADVLA F+AAI DG D++SVSLG +   F  D
Sbjct: 250 TAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFND 309

Query: 311 LMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVALGDNRH 370
            +AIGSFHA K  I VVCSAGNSG  + +VSNVAPW ITVGAST+DR F + + LG+ +H
Sbjct: 310 SVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKH 369

Query: 371 FKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVCLNSYD 430
           +KG+SLSS  LP  KFYP++ +++A + N S+ ++ LC  GSLDP K KGKI+VCL   +
Sbjct: 370 YKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQN 429

Query: 431 SEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQSTITPM 490
             V+KG   A  G +GM+L N   +G+DL    H+LPA+ ++  D  +++ YI  T  P+
Sbjct: 430 GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPI 489

Query: 491 AYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASPTDSPF 550
           A+IT  +T+LG+KPAP+MASFSS+GP+ V P ILKPDITAPGV+++AA++G  SPT+  F
Sbjct: 490 AHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQF 549

Query: 551 DKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLNPILNS 610
           D RR+L+N +SGTSMSCPHISGI GLLKT YP WSPAAI+SAIMTTA    +   PI N+
Sbjct: 550 DPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNA 609

Query: 611 EKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDTSFVCS 670
              KA P ++GAGH+QPN A NPGL+YDL  +DYLNFLC  GYN +Q+ +F+ +     S
Sbjct: 610 TNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSS 669

Query: 671 KSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPSTLKFT 730
              ++ +LNYPSI++ NL S  V + R VKNVG PS Y  +V  P GV V+V P++L FT
Sbjct: 670 PKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFT 729

Query: 731 KTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTL 771
           K GE+K FKV+L +   N  +GY+FG+LVWS+  H V SPI V L
Sbjct: 730 KVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmoCh16G010230 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 1.1e-251
Identity = 444/770 (57.66%), Postives = 553/770 (71.82%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           M   +L  LLL   L  L  +   A +KSYIVYLGSH+H    S+  L  V  +H   L 
Sbjct: 16  MSLQSLSSLLL---LVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLA 75

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           S +GS+E AKE+IFYSY ++INGFAA+L+   A +IAKHPDV+SV+ NKG KLHTT SWN
Sbjct: 76  SFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWN 135

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           F+ +  +G +   SLWN + +GE TII N+DTGVWPESKSFSDEGYG +P RWKG C   
Sbjct: 136 FMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
            +  CNRKLIGARYFNKGY+ +    P N+SYET RD+DGHG+HTLSTA GNFVPG ++F
Sbjct: 196 KDVPCNRKLIGARYFNKGYL-AYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF 255

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           G G GTA GGSP A VAAYKVCWPP    ECF AD+LA  EAAI DGVD+LS S+G DA 
Sbjct: 256 GIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAG 315

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           ++  D +AIGSFHAVKNG+TVVCSAGNSG   G+VSNVAPW+ITVGAS++DR F  +V L
Sbjct: 316 DYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVEL 375

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
            + + FKG SL SK LP +K Y LI A DA   N +  ++ LC KGSLDPKKVKGKI+VC
Sbjct: 376 KNGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVC 435

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           L   ++ VDKG +AA AGA GM+L ND+ SG+++ +  H+LPAS + Y DGE++  Y+ S
Sbjct: 436 LRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSS 495

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           T  P  YI      L  KPAP MASFSSRGPN + P ILKPDITAPGVNI+AAF+    P
Sbjct: 496 TKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGP 555

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           TD   D RR  +N  SGTSMSCPHISG+VGLLKTL+P WSPAAI+SAIMTT+ T  N   
Sbjct: 556 TDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRK 615

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           P+++   +KANP +YG+GH+QPNKAA+PGL+YDL+T DYL+FLC  GYN T ++LF  D 
Sbjct: 616 PMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDP 675

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
            + C +   + D NYPSI++ NL + ++ + R++KNVG P+TY A+   P GV VSV P 
Sbjct: 676 QYTCRQGANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPK 735

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTL 771
            L F KTGE K F++ LR +P   + GY+FG+L W++ +H+V SPI V L
Sbjct: 736 QLTFNKTGEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CmoCh16G010230 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 720.3 bits (1858), Expect = 2.3e-206
Identity = 395/788 (50.13%), Postives = 515/788 (65.36%), Query Frame = 0

Query: 4   FNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLGSVL 63
           F L  +L  FFLF  L  +   ++K YIVY+G+HSHG +P++ DL+L T +HY+LLGS+ 
Sbjct: 6   FCLHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIF 65

Query: 64  GSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWNFLG 123
           GS E AKE+I YSYN++INGFAA+LE + A DIAK+P+V+SV+ +K  KLHTTRSW FLG
Sbjct: 66  GSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLG 125

Query: 124 VENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGS-CE---- 183
           +   G    +S W   RFGE+TIIGNIDTGVWPES+SFSD+GYG +P++W+G  C+    
Sbjct: 126 LHRRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKL 185

Query: 184 -GGSNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGV 243
            G     CNRKLIGARY+NK +        L+    TARD+ GHGTHTLSTAGGNFVPG 
Sbjct: 186 PGSMKNTCNRKLIGARYYNKAF--EAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGA 245

Query: 244 SIFGNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGE 303
            +F  G GTAKGGSP A VAAYKVCW  T    C+ ADVLA  + AI DGVD+++VS G 
Sbjct: 246 RVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGV 305

Query: 304 DAFEFSE----DLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRL 363
                +E    D ++IG+FHA+   I +V SAGN G   G+V+NVAPW+ T+ AST+DR 
Sbjct: 306 SYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRD 365

Query: 364 FTTYVALGDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKV 423
           F++ + + +N+  +G SL    LP  + + LI + DA   N +  ++ LC +G+LD  KV
Sbjct: 366 FSSNLTI-NNQLIEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKV 425

Query: 424 KGKIVVCLNSYD-SEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGE 483
            GKIV+C        V +G EA  AGA GMIL N  ++G  L    H+    +      +
Sbjct: 426 NGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAK 485

Query: 484 SINHYIQSTIT----------PMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPD 543
           S  H +++T                ++R +T  G KPAP+MASFSSRGPN+++PSILKPD
Sbjct: 486 SRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPD 545

Query: 544 ITAPGVNILAAFSGKASPTDSPFDKRR-VLYNVLSGTSMSCPHISGIVGLLKTLYPKWSP 603
           +TAPGVNILAA+S  AS +    D RR   +NVL GTSMSCPH SGI GLLKT +P WSP
Sbjct: 546 VTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSP 605

Query: 604 AAIKSAIMTTAETEANDLNPILNS-EKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYL 663
           AAIKSAIMTTA T  N   PI ++ +K  A+  AYG+GH++P+ A  PGL+YDLS  DYL
Sbjct: 606 AAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYL 665

Query: 664 NFLCVRGYNKTQMKLFTNDTSFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSP 723
           NFLC  GY++  +     + +F+CS S +V DLNYPSI++ NL+ + V I R V NVG P
Sbjct: 666 NFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPP 725

Query: 724 STYVAQVEAPPGVSVSVNPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNH 769
           STY     +P G S++V P +L FTK GE K FKV+++         Y FG L W++G H
Sbjct: 726 STYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKH 785

BLAST of CmoCh16G010230 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 3.5e-170
Identity = 340/774 (43.93%), Postives = 473/774 (61.11%), Query Frame = 0

Query: 13  FFLFALL----QTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLGSVLGSNEA 72
           FFL   L     +S+ + Q +YIV++        PS+ DL      H N   S L S   
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISD 70

Query: 73  AKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWNFLGVENDG 132
           + E + Y+Y   I+GF+  L  + A+ +   P VISV      +LHTTR+  FLG++   
Sbjct: 71  SAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH- 130

Query: 133 GIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGSNFR---C 192
              +  L+  +      ++G +DTGVWPESKS+SDEG+GPIP+ WKG CE G+NF    C
Sbjct: 131 ---TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 190

Query: 193 NRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIFGNGYG 252
           NRKLIGAR+F +GY S++ P   +    + RD DGHGTHT STA G+ V G S+ G   G
Sbjct: 191 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 250

Query: 253 TAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAFEFSED 312
           TA+G +P A VA YKVCW     G CF +D+LA  + AI+D V++LS+SLG    ++  D
Sbjct: 251 TARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 310

Query: 313 LMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVALGDNRH 372
            +AIG+F A++ GI V CSAGN+G    S+SNVAPW+ TVGA T+DR F     LG+ ++
Sbjct: 311 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 370

Query: 373 ------FKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVV 432
                 FKGE+L  K+LPF   Y        A +  ++ N  LC+ G+L P+KVKGKIV+
Sbjct: 371 FTGVSLFKGEALPDKLLPF--IY--------AGNASNATNGNLCMTGTLIPEKVKGKIVM 430

Query: 433 CLNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQ 492
           C    ++ V KG     AG VGMIL N   +G++L    HLLPA+ V    G+ I HY+ 
Sbjct: 431 CDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT 490

Query: 493 STITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKAS 552
           +   P A I+ + T +G+KP+P++A+FSSRGPN + P+ILKPD+ APGVNILAA++G A 
Sbjct: 491 TDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAG 550

Query: 553 PTDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDL 612
           PT    D RRV +N++SGTSMSCPH+SG+  LLK+++P+WSPAAI+SA+MTTA     D 
Sbjct: 551 PTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDG 610

Query: 613 NPILNSEKEK-ANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTN 672
            P+L+    K + P  +GAGH+ P  A NPGLIYDL+T+DYL FLC   Y   Q++  + 
Sbjct: 611 KPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR 670

Query: 673 DTSFVC--SKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEA-PPGVSV 732
             ++ C  SKS++V DLNYPS ++N     A +  R V +VG   TY  +V +   GV +
Sbjct: 671 -RNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKI 730

Query: 733 SVNPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVT 770
           SV P+ L F +  E+K + V      +  +    FG + WS+G H V SP+ ++
Sbjct: 731 SVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmoCh16G010230 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 2.2e-156
Identity = 334/799 (41.80%), Postives = 468/799 (58.57%), Query Frame = 0

Query: 10  LLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLGSVLGSNEAA 69
           L P      L  S    ++ YIVY G H             + + H++ L SV  S E A
Sbjct: 7   LFPLLFLIPLLASCAEEKQVYIVYFGEHK-----GDKAFHEIEEHHHSYLQSVKESEEDA 66

Query: 70  KESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYEN--KGLKLHTTRSWNFLGV--- 129
           + S+ YSY  +INGFAA L    A  + K  +V+SV+++  +  + HTTRSW F+G+   
Sbjct: 67  RASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEE 126

Query: 130 ENDGGIP-----SDSLWNLSR-------FGESTIIGNIDTGVWPESKSFSDEGYGPIPTR 189
           E D  +P     +D  + + R        G+  I+G +D+GVWPESKSF+D+G GP+P  
Sbjct: 127 ETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKS 186

Query: 190 WKGSCEGGSNF---RCNRKLIGARYFNKGY--ISSVEPRPLNSSYETARDYDGHGTHTLS 249
           WKG C+ G  F    CNRK+IGARY+ KGY           N  + + RD DGHG+HT S
Sbjct: 187 WKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTAS 246

Query: 250 TAGGNFVPGVSIFGN-GYGTAKGGSPNALVAAYKVCW----PPTLDGE-CFMADVLAGFE 309
           TA G  V G S  G    G+A GG+P A +A YK CW       ++G  C   D+LA  +
Sbjct: 247 TAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAID 306

Query: 310 AAISDGVDILSVSLG-EDAFEFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAP 369
            AI+DGV ++S+S+G  + F F++D +A+G+ HAVK  I V  SAGNSG   G++SN+AP
Sbjct: 307 DAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAP 366

Query: 370 WMITVGASTIDRLFTTYVALGDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNS 429
           W+ITVGAST+DR F   + LG+    K +S+++      KF PL+ A +     I+ N +
Sbjct: 367 WIITVGASTLDRAFVGGLVLGNGYTIKTDSITA--FKMDKFAPLVYASNVVVPGIALNET 426

Query: 430 ALCLKGSLDPKKVKGKIVVCLNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHL 489
           + CL  SL P+ V GK+V+CL    S + KG E  +AG  GMIL N   +G+++ +  H 
Sbjct: 427 SQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHF 486

Query: 490 LPASHVSYIDGESINHYIQSTITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILK 549
           +P + V+    + I  YI++   P A+I   KT    + AP M  FSSRGPN V+P+ILK
Sbjct: 487 VPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILK 546

Query: 550 PDITAPGVNILAAFSGKASPTDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWS 609
           PDITAPG+ ILAA+SG  SP+    D+R   YN+ SGTSMSCPH++G + LLK ++PKWS
Sbjct: 547 PDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 606

Query: 610 PAAIKSAIMTTAETEANDLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYL 669
            AAI+SA+MTTA    +   PI ++    ANP A G+GH +P KAA+PGL+YD S + YL
Sbjct: 607 SAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 670 NFLCVRGYNKTQMKLFTNDTSFVC-SKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVG- 729
            + C        + +   D +F C SK     + NYPSI++ NLK + V +KR V NVG 
Sbjct: 667 LYGC-------SVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLK-KTVTVKRTVTNVGT 726

Query: 730 --SPSTYVAQVEAPPGVSVSVNPSTLKFTKTGEEKDFKVVLRRVPN---NQTE--GYMFG 771
             S STY+  V+ P G+SV   P+ L F + G+++ FK+V++ + N   N TE   Y FG
Sbjct: 727 GNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFG 786

BLAST of CmoCh16G010230 vs. ExPASy TrEMBL
Match: A0A6J1EWA7 (subtilisin-like protease SBT5.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111438766 PE=3 SV=1)

HSP 1 Score: 1552.0 bits (4017), Expect = 0.0e+00
Identity = 773/773 (100.00%), Postives = 773/773 (100.00%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF
Sbjct: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL
Sbjct: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC
Sbjct: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS
Sbjct: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP
Sbjct: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN
Sbjct: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT
Sbjct: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS
Sbjct: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ
Sbjct: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 773

BLAST of CmoCh16G010230 vs. ExPASy TrEMBL
Match: A0A6J1F243 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438766 PE=3 SV=1)

HSP 1 Score: 1546.6 bits (4003), Expect = 0.0e+00
Identity = 773/776 (99.61%), Postives = 773/776 (99.61%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF
Sbjct: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL
Sbjct: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC
Sbjct: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS
Sbjct: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP
Sbjct: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTT---AETEAN 600
           TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTT   AETEAN
Sbjct: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTGSAAETEAN 600

Query: 601 DLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFT 660
           DLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFT
Sbjct: 601 DLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFT 660

Query: 661 NDTSFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSV 720
           NDTSFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSV
Sbjct: 661 NDTSFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSV 720

Query: 721 NPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           NPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ
Sbjct: 721 NPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 776

BLAST of CmoCh16G010230 vs. ExPASy TrEMBL
Match: A0A6J1EWB2 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438771 PE=3 SV=1)

HSP 1 Score: 1325.5 bits (3429), Expect = 0.0e+00
Identity = 661/773 (85.51%), Postives = 710/773 (91.85%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFF+FALLQTSTIAA+KSYIVYLGSHSHGLNPSALDLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFIFALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLTTQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKE+IFYSYN+NINGFAAVL+  VAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKEAIFYSYNRNINGFAAVLDQNVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGG+PS+SLWNLS FGESTIIGNID+GV PESKSFSDEGYGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDSGVRPESKSFSDEGYGPIPKRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYIS VEP+PLNS++ETARD+DGHGTHTLSTAGGNFV GV+IF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISIVEPKPLNSTFETARDHDGHGTHTLSTAGGNFVEGVNIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSP ALVAAY+VCWP TLDG CF+ADV+AGFEAAISDGVD+LSVSLG DA 
Sbjct: 241 GNGYGTAKGGSPKALVAAYRVCWPQTLDGACFIADVVAGFEAAISDGVDVLSVSLGGDAT 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFS DLMAI SFHAVKNGITVVCSAGNSG  E  VSNVAPWMITVGASTIDRLFTTYV L
Sbjct: 301 EFSNDLMAIMSFHAVKNGITVVCSAGNSGPIESIVSNVAPWMITVGASTIDRLFTTYVVL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           G+    KGESLS++ILP  KFYPLIR+LDA SDN+S+ ++  C KG+LDPKKVKGKIVVC
Sbjct: 361 GNKMRLKGESLSNQILPANKFYPLIRSLDAKSDNVSTYDAQTCEKGTLDPKKVKGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           +N  DS + K Y AAQAGAVG+ILVN E+ GD+L  A HLLPA+H+SY D +SI+ YIQS
Sbjct: 421 VNVGDS-MAKSYVAAQAGAVGIILVNYEDIGDELLPAAHLLPAAHISYTDRKSIDQYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           T +PMAYITRVKTE GIKPAPIMASFSSRGPN VEPSILKPDITAPGVNILAAFS +ASP
Sbjct: 481 TKSPMAYITRVKTESGIKPAPIMASFSSRGPNPVEPSILKPDITAPGVNILAAFSDEASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           T SPFDKR++LY VLSGTSMSCPHISGIVGLLKTLYPKWSPAAI+SAIMTTAET+ANDLN
Sbjct: 541 TGSPFDKRQILYTVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PILN EKEK NPLAYGAGH+QPNKAA+PGL+YDLSTQDYLNFLC RGYNKT MKLFT+DT
Sbjct: 601 PILNPEKEKVNPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCARGYNKTLMKLFTSDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVCSKSF VTDLNYPSISMNNLKSE VEIKRRVKNVGSP TYVAQVEAPPGVSVSV+PS
Sbjct: 661 SFVCSKSFKVTDLNYPSISMNNLKSEVVEIKRRVKNVGSPGTYVAQVEAPPGVSVSVDPS 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTKT EEKDFKVVLRRVPNNQT+GY+FGKLVWS+G H VSSPIFV L RQ
Sbjct: 721 TLKFTKTDEEKDFKVVLRRVPNNQTDGYVFGKLVWSDGKHRVSSPIFVILRRQ 772

BLAST of CmoCh16G010230 vs. ExPASy TrEMBL
Match: A0A6J1KZG2 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500238 PE=3 SV=1)

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 652/773 (84.35%), Postives = 708/773 (91.59%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFFLFALLQTSTIAA+KSYIVYLGSHSHGLNPSALDLQL TQ+HYNLLG
Sbjct: 1   MEAFNLPPLLLPFFLFALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLTTQSHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKE+IFYSYN+NINGFAAVL+ KVAE +AKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKEAIFYSYNRNINGFAAVLDQKVAEVVAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGG+PS+SLWNLS FGESTIIGNIDTGVWPESKSFSDEGYGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPKRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYIS VEP+PLNSSYETARD+DGHGTHTLSTAGGNFV GVSIF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISVVEPKPLNSSYETARDHDGHGTHTLSTAGGNFVEGVSIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSP ALVAAY+VCWP TL G CF+AD++AGFEAAISDGVD+LSVSLG DA 
Sbjct: 241 GNGYGTAKGGSPKALVAAYRVCWPQTLYGACFIADIVAGFEAAISDGVDVLSVSLGGDAT 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFS DLMAI SFHAVKNGITVVCSAGNSG  E  V+NVAPWMITVGASTIDR FTTYV L
Sbjct: 301 EFSNDLMAIMSFHAVKNGITVVCSAGNSGPTESIVTNVAPWMITVGASTIDRHFTTYVVL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           G+    KGESLS++ILP KKFYPLI +LDA  +N+S+ ++  C KG+LDPKKV+GKIVVC
Sbjct: 361 GNKTRLKGESLSNQILPAKKFYPLIHSLDAKLNNVSTYDAQTCEKGALDPKKVQGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           +N  D  + K Y AAQAGA+G+ILVN E+ GD+L  A HLLPA+H+SY D +SI+ YIQS
Sbjct: 421 VNVGD-PMAKSYVAAQAGAIGIILVNYEDIGDELLPAAHLLPAAHISYTDRKSIDQYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           T +PMAYITRVKTELG+KPAPIMASFSSRGPN +EPSILKPDITAPGVNILAAFS +ASP
Sbjct: 481 TKSPMAYITRVKTELGVKPAPIMASFSSRGPNPIEPSILKPDITAPGVNILAAFSDEASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           T SPFDKRR+LY VLSGTSMSCPHISG+VGLLKTLYPKWSPAAI+SAIMTTAET+ANDLN
Sbjct: 541 TGSPFDKRRILYTVLSGTSMSCPHISGVVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PILN EKEK NPLAYGAGH+QPNKAANPGL+YDLSTQDYLNFLC  GYNKTQMKLF+NDT
Sbjct: 601 PILNPEKEKVNPLAYGAGHVQPNKAANPGLVYDLSTQDYLNFLCAHGYNKTQMKLFSNDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVCSKS  VTDLNYPSISMNNLKS+ VE+KRRVKNVGSP TYVAQVEAPPGVSVSV+P+
Sbjct: 661 SFVCSKSVKVTDLNYPSISMNNLKSKEVEVKRRVKNVGSPGTYVAQVEAPPGVSVSVDPN 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTKTGEEKDFKVVLRRVPNNQT+GY+FGKLVW +G H VSSPIFVTL R+
Sbjct: 721 TLKFTKTGEEKDFKVVLRRVPNNQTDGYVFGKLVWYDGKHRVSSPIFVTLTRE 772

BLAST of CmoCh16G010230 vs. ExPASy TrEMBL
Match: A0A6J1L8E9 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111500239 PE=3 SV=1)

HSP 1 Score: 1286.2 bits (3327), Expect = 0.0e+00
Identity = 637/773 (82.41%), Postives = 695/773 (89.91%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFFLFALLQTSTIAA+KSYIVYLGSHSHG NPSALDLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFLFALLQTSTIAAKKSYIVYLGSHSHGFNPSALDLQLATQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKE+IFYSYN+NINGFAAVL+ KVAE +AKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKEAIFYSYNRNINGFAAVLDQKVAEVVAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGG+PS+SLWNLS FGESTIIGNIDTGVWPESKSFSDEGYGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDTGVWPESKSFSDEGYGPIPKRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYIS VEP+PLNSSYET RD DGHGTHTLSTAGGNFV GVS F
Sbjct: 181 SNFRCNRKLIGARYFNKGYISVVEPKPLNSSYETTRDDDGHGTHTLSTAGGNFVDGVSFF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNG GTAKGGSP A VAAYKVCWPP L G CFMAD++AGFEAAISDGVD+LSVSLG    
Sbjct: 241 GNGNGTAKGGSPKARVAAYKVCWPPALYGSCFMADIVAGFEAAISDGVDVLSVSLGGLPM 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFS+DLMA+ SFHAVKNGITVVCS GN G  E SVSN+APWMITVGASTIDRLFTTYV L
Sbjct: 301 EFSDDLMAVVSFHAVKNGITVVCSGGNFGPVEKSVSNIAPWMITVGASTIDRLFTTYVVL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           G+    KGESLS++ILP KKFYPLIR+LDA  +N   N++  C KGSLDPKKVKGKIV+C
Sbjct: 361 GNKMRLKGESLSNQILPAKKFYPLIRSLDAKFNNTLPNDAQTCAKGSLDPKKVKGKIVIC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           +N  D+ ++K +  AQAGAVG+ILVN E+ GD L  A HL+PA+H+SY D +SI+ YIQS
Sbjct: 421 VNLGDA-MEKSHVVAQAGAVGIILVNYEDIGDGLLPAAHLIPAAHISYTDRKSIDQYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           T +PMAY+TRVKTELGIKPAPIMASFSSRGP+ +EP ILKPDITAPGVNILAAFS + SP
Sbjct: 481 TKSPMAYMTRVKTELGIKPAPIMASFSSRGPSLIEPLILKPDITAPGVNILAAFSDEVSP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           T SPFDKRRV YNVLSGTSMSCPHISGIVG LKTLYPKWSPAA++SAIMTTAET+ANDLN
Sbjct: 541 TGSPFDKRRVQYNVLSGTSMSCPHISGIVGQLKTLYPKWSPAALRSAIMTTAETKANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PILN EKEK +PLAYGAGH+QPNKAA+PGL+YDLSTQDYLNFLC RGYNKT MKLFTNDT
Sbjct: 601 PILNPEKEKVDPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCARGYNKTLMKLFTNDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVC KSF VTDLNYPSISMN LKSEAVE+KRRV NVGSP TYVAQ+EAPP VS+SV+PS
Sbjct: 661 SFVCLKSFKVTDLNYPSISMNYLKSEAVEVKRRVTNVGSPGTYVAQIEAPPEVSISVDPS 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTKTGEEKDFKVVL++V NNQT+GY+FGKLVWS+G H VSSPIFV+L ++
Sbjct: 721 TLKFTKTGEEKDFKVVLKKVSNNQTDGYVFGKLVWSDGKHSVSSPIFVSLTKK 772

BLAST of CmoCh16G010230 vs. NCBI nr
Match: XP_022932411.1 (subtilisin-like protease SBT5.3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1552.0 bits (4017), Expect = 0.0e+00
Identity = 773/773 (100.00%), Postives = 773/773 (100.00%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF
Sbjct: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL
Sbjct: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC
Sbjct: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS
Sbjct: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP
Sbjct: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN
Sbjct: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT
Sbjct: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS
Sbjct: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ
Sbjct: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 773

BLAST of CmoCh16G010230 vs. NCBI nr
Match: XP_022932410.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1546.6 bits (4003), Expect = 0.0e+00
Identity = 773/776 (99.61%), Postives = 773/776 (99.61%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF
Sbjct: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL
Sbjct: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC
Sbjct: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS
Sbjct: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP
Sbjct: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTT---AETEAN 600
           TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTT   AETEAN
Sbjct: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTGSAAETEAN 600

Query: 601 DLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFT 660
           DLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFT
Sbjct: 601 DLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFT 660

Query: 661 NDTSFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSV 720
           NDTSFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSV
Sbjct: 661 NDTSFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSV 720

Query: 721 NPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           NPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ
Sbjct: 721 NPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 776

BLAST of CmoCh16G010230 vs. NCBI nr
Match: KAG6577537.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1506.9 bits (3900), Expect = 0.0e+00
Identity = 749/773 (96.90%), Postives = 760/773 (98.32%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFFLFALLQTSTI A+KSYIVYLGSHSHGLNPSA DLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFLFALLQTSTIEAKKSYIVYLGSHSHGLNPSAFDLQLATQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGS EAAKESIFYSYNKNINGFAAVLE KVAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSKEAAKESIFYSYNKNINGFAAVLEQKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGGIPS SLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDGGIPSGSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYISSVEPRPLNSSY+TARDYDGHGTHTLSTAGGNFVPGVSIF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYDTARDYDGHGTHTLSTAGGNFVPGVSIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF
Sbjct: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFS+DLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL
Sbjct: 301 EFSKDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           GDNRHFKGESLSSKILP KKFYPLIRALDAASDNIS+NNSALCLKGSLDPKKVKGKIVVC
Sbjct: 361 GDNRHFKGESLSSKILPSKKFYPLIRALDAASDNISNNNSALCLKGSLDPKKVKGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS
Sbjct: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           T+TP+AYIT VKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFS KASP
Sbjct: 481 TVTPIAYITHVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSNKASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAA++SAIMTTAETEANDLN
Sbjct: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAMRSAIMTTAETEANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFT+DT
Sbjct: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTDDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVCSKSF VTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVS++PS
Sbjct: 661 SFVCSKSFKVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSIDPS 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTK GEEKDFKVVLRRVPNNQTE YMFGKLVWSNGNH VSSPIFVTLERQ
Sbjct: 721 TLKFTKIGEEKDFKVVLRRVPNNQTERYMFGKLVWSNGNHRVSSPIFVTLERQ 773

BLAST of CmoCh16G010230 vs. NCBI nr
Match: XP_023553306.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1481.5 bits (3834), Expect = 0.0e+00
Identity = 737/773 (95.34%), Postives = 751/773 (97.15%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLL FFL A+LQT T+AA+KSYIVYLGSHSHGLNPSA DLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLSFFLCAILQTPTMAAKKSYIVYLGSHSHGLNPSAHDLQLATQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKESIFYSYNKNINGFAAVLE KVAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEQKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVEND GIPS SLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG
Sbjct: 121 FLGVENDSGIPSSSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSPNALVAAYKVCWPPTLDGECF+ADVLAGFEA ISDGVD+LSVSLGEDAF
Sbjct: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFIADVLAGFEAVISDGVDVLSVSLGEDAF 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPW+ITVGASTIDRLFTTYVAL
Sbjct: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWIITVGASTIDRLFTTYVAL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           GD RHFKGESLSSKI P KKFYPLIRALDA SDN+SSN+SALCLKG+LDPKKVKGKIVV 
Sbjct: 361 GDKRHFKGESLSSKIFPSKKFYPLIRALDATSDNVSSNDSALCLKGTLDPKKVKGKIVVY 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           LNSYDSEVDKGYEAAQAGAVGMILVNDEES DDLWTAEHLLPASHVSYIDGESINHYIQS
Sbjct: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESRDDLWTAEHLLPASHVSYIDGESINHYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFS +ASP
Sbjct: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSDEASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           T SPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAI+SAIMTTA TEANDLN
Sbjct: 541 THSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PI NSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLC RGYNKTQMKLF NDT
Sbjct: 601 PIQNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCARGYNKTQMKLFINDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVCSKSFT+TDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVS+SV+PS
Sbjct: 661 SFVCSKSFTITDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSISVDPS 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNH VSSPIFVTLERQ
Sbjct: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHRVSSPIFVTLERQ 773

BLAST of CmoCh16G010230 vs. NCBI nr
Match: XP_022932416.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1325.5 bits (3429), Expect = 0.0e+00
Identity = 661/773 (85.51%), Postives = 710/773 (91.85%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           MEAFNLPPLLLPFF+FALLQTSTIAA+KSYIVYLGSHSHGLNPSALDLQL TQTHYNLLG
Sbjct: 1   MEAFNLPPLLLPFFIFALLQTSTIAAKKSYIVYLGSHSHGLNPSALDLQLTTQTHYNLLG 60

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           SVLGSNEAAKE+IFYSYN+NINGFAAVL+  VAEDIAKHPDVISVYENKGLKLHTTRSWN
Sbjct: 61  SVLGSNEAAKEAIFYSYNRNINGFAAVLDQNVAEDIAKHPDVISVYENKGLKLHTTRSWN 120

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           FLGVENDGG+PS+SLWNLS FGESTIIGNID+GV PESKSFSDEGYGPIP RWKGSCEGG
Sbjct: 121 FLGVENDGGVPSNSLWNLSSFGESTIIGNIDSGVRPESKSFSDEGYGPIPKRWKGSCEGG 180

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
           SNFRCNRKLIGARYFNKGYIS VEP+PLNS++ETARD+DGHGTHTLSTAGGNFV GV+IF
Sbjct: 181 SNFRCNRKLIGARYFNKGYISIVEPKPLNSTFETARDHDGHGTHTLSTAGGNFVEGVNIF 240

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           GNGYGTAKGGSP ALVAAY+VCWP TLDG CF+ADV+AGFEAAISDGVD+LSVSLG DA 
Sbjct: 241 GNGYGTAKGGSPKALVAAYRVCWPQTLDGACFIADVVAGFEAAISDGVDVLSVSLGGDAT 300

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           EFS DLMAI SFHAVKNGITVVCSAGNSG  E  VSNVAPWMITVGASTIDRLFTTYV L
Sbjct: 301 EFSNDLMAIMSFHAVKNGITVVCSAGNSGPIESIVSNVAPWMITVGASTIDRLFTTYVVL 360

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
           G+    KGESLS++ILP  KFYPLIR+LDA SDN+S+ ++  C KG+LDPKKVKGKIVVC
Sbjct: 361 GNKMRLKGESLSNQILPANKFYPLIRSLDAKSDNVSTYDAQTCEKGTLDPKKVKGKIVVC 420

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           +N  DS + K Y AAQAGAVG+ILVN E+ GD+L  A HLLPA+H+SY D +SI+ YIQS
Sbjct: 421 VNVGDS-MAKSYVAAQAGAVGIILVNYEDIGDELLPAAHLLPAAHISYTDRKSIDQYIQS 480

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           T +PMAYITRVKTE GIKPAPIMASFSSRGPN VEPSILKPDITAPGVNILAAFS +ASP
Sbjct: 481 TKSPMAYITRVKTESGIKPAPIMASFSSRGPNPVEPSILKPDITAPGVNILAAFSDEASP 540

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           T SPFDKR++LY VLSGTSMSCPHISGIVGLLKTLYPKWSPAAI+SAIMTTAET+ANDLN
Sbjct: 541 TGSPFDKRQILYTVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAETKANDLN 600

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           PILN EKEK NPLAYGAGH+QPNKAA+PGL+YDLSTQDYLNFLC RGYNKT MKLFT+DT
Sbjct: 601 PILNPEKEKVNPLAYGAGHVQPNKAADPGLVYDLSTQDYLNFLCARGYNKTLMKLFTSDT 660

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
           SFVCSKSF VTDLNYPSISMNNLKSE VEIKRRVKNVGSP TYVAQVEAPPGVSVSV+PS
Sbjct: 661 SFVCSKSFKVTDLNYPSISMNNLKSEVVEIKRRVKNVGSPGTYVAQVEAPPGVSVSVDPS 720

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTLERQ 774
           TLKFTKT EEKDFKVVLRRVPNNQT+GY+FGKLVWS+G H VSSPIFV L RQ
Sbjct: 721 TLKFTKTDEEKDFKVVLRRVPNNQTDGYVFGKLVWSDGKHRVSSPIFVILRRQ 772

BLAST of CmoCh16G010230 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 870.9 bits (2249), Expect = 7.5e-253
Identity = 432/765 (56.47%), Postives = 553/765 (72.29%), Query Frame = 0

Query: 11  LPFFLFALLQTSTIAAQK---SYIVYLGSHSHGLNPSALDLQLVTQTHYNLLGSVLGSNE 70
           L   L   + +  I A K   SY+VY G+HSH    +   +  V +THY+ LGS  GS E
Sbjct: 10  LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69

Query: 71  AAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWNFLGVEND 130
            A ++IFYSY K+INGFAA L+H +A +I+KHP+V+SV+ NK LKLHTTRSW+FLG+E++
Sbjct: 70  RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129

Query: 131 GGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEG--GSNFRC 190
             +PS S+W  +RFGE TII N+DTGVWPESKSF DEG GPIP+RWKG C+    + F C
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHC 189

Query: 191 NRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIFGNGYG 250
           NRKLIGARYFNKGY ++V    LNSS+++ RD DGHG+HTLSTA G+FVPGVSIFG G G
Sbjct: 190 NRKLIGARYFNKGYAAAV--GHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 249

Query: 251 TAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAFEFSED 310
           TAKGGSP A VAAYKVCWPP    EC+ ADVLA F+AAI DG D++SVSLG +   F  D
Sbjct: 250 TAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFND 309

Query: 311 LMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVALGDNRH 370
            +AIGSFHA K  I VVCSAGNSG  + +VSNVAPW ITVGAST+DR F + + LG+ +H
Sbjct: 310 SVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKH 369

Query: 371 FKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVCLNSYD 430
           +KG+SLSS  LP  KFYP++ +++A + N S+ ++ LC  GSLDP K KGKI+VCL   +
Sbjct: 370 YKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQN 429

Query: 431 SEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQSTITPM 490
             V+KG   A  G +GM+L N   +G+DL    H+LPA+ ++  D  +++ YI  T  P+
Sbjct: 430 GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPI 489

Query: 491 AYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASPTDSPF 550
           A+IT  +T+LG+KPAP+MASFSS+GP+ V P ILKPDITAPGV+++AA++G  SPT+  F
Sbjct: 490 AHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQF 549

Query: 551 DKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLNPILNS 610
           D RR+L+N +SGTSMSCPHISGI GLLKT YP WSPAAI+SAIMTTA    +   PI N+
Sbjct: 550 DPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNA 609

Query: 611 EKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDTSFVCS 670
              KA P ++GAGH+QPN A NPGL+YDL  +DYLNFLC  GYN +Q+ +F+ +     S
Sbjct: 610 TNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSS 669

Query: 671 KSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPSTLKFT 730
              ++ +LNYPSI++ NL S  V + R VKNVG PS Y  +V  P GV V+V P++L FT
Sbjct: 670 PKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFT 729

Query: 731 KTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTL 771
           K GE+K FKV+L +   N  +GY+FG+LVWS+  H V SPI V L
Sbjct: 730 KVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmoCh16G010230 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 870.9 bits (2249), Expect = 7.5e-253
Identity = 444/770 (57.66%), Postives = 553/770 (71.82%), Query Frame = 0

Query: 1   MEAFNLPPLLLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLG 60
           M   +L  LLL   L  L  +   A +KSYIVYLGSH+H    S+  L  V  +H   L 
Sbjct: 16  MSLQSLSSLLL---LVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLA 75

Query: 61  SVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWN 120
           S +GS+E AKE+IFYSY ++INGFAA+L+   A +IAKHPDV+SV+ NKG KLHTT SWN
Sbjct: 76  SFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWN 135

Query: 121 FLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGG 180
           F+ +  +G +   SLWN + +GE TII N+DTGVWPESKSFSDEGYG +P RWKG C   
Sbjct: 136 FMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-- 195

Query: 181 SNFRCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIF 240
            +  CNRKLIGARYFNKGY+ +    P N+SYET RD+DGHG+HTLSTA GNFVPG ++F
Sbjct: 196 KDVPCNRKLIGARYFNKGYL-AYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF 255

Query: 241 GNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAF 300
           G G GTA GGSP A VAAYKVCWPP    ECF AD+LA  EAAI DGVD+LS S+G DA 
Sbjct: 256 GIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAG 315

Query: 301 EFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVAL 360
           ++  D +AIGSFHAVKNG+TVVCSAGNSG   G+VSNVAPW+ITVGAS++DR F  +V L
Sbjct: 316 DYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVEL 375

Query: 361 GDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVVC 420
            + + FKG SL SK LP +K Y LI A DA   N +  ++ LC KGSLDPKKVKGKI+VC
Sbjct: 376 KNGQSFKGTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVC 435

Query: 421 LNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQS 480
           L   ++ VDKG +AA AGA GM+L ND+ SG+++ +  H+LPAS + Y DGE++  Y+ S
Sbjct: 436 LRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSS 495

Query: 481 TITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKASP 540
           T  P  YI      L  KPAP MASFSSRGPN + P ILKPDITAPGVNI+AAF+    P
Sbjct: 496 TKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGP 555

Query: 541 TDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDLN 600
           TD   D RR  +N  SGTSMSCPHISG+VGLLKTL+P WSPAAI+SAIMTT+ T  N   
Sbjct: 556 TDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRK 615

Query: 601 PILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTNDT 660
           P+++   +KANP +YG+GH+QPNKAA+PGL+YDL+T DYL+FLC  GYN T ++LF  D 
Sbjct: 616 PMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDP 675

Query: 661 SFVCSKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEAPPGVSVSVNPS 720
            + C +   + D NYPSI++ NL + ++ + R++KNVG P+TY A+   P GV VSV P 
Sbjct: 676 QYTCRQGANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPK 735

Query: 721 TLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVTL 771
            L F KTGE K F++ LR +P   + GY+FG+L W++ +H+V SPI V L
Sbjct: 736 QLTFNKTGEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CmoCh16G010230 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 600.1 bits (1546), Expect = 2.5e-171
Identity = 340/774 (43.93%), Postives = 473/774 (61.11%), Query Frame = 0

Query: 13  FFLFALL----QTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLGSVLGSNEA 72
           FFL   L     +S+ + Q +YIV++        PS+ DL      H N   S L S   
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQ---MPSSFDL------HSNWYDSSLRSISD 70

Query: 73  AKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLHTTRSWNFLGVENDG 132
           + E + Y+Y   I+GF+  L  + A+ +   P VISV      +LHTTR+  FLG++   
Sbjct: 71  SAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH- 130

Query: 133 GIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWKGSCEGGSNFR---C 192
              +  L+  +      ++G +DTGVWPESKS+SDEG+GPIP+ WKG CE G+NF    C
Sbjct: 131 ---TADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 190

Query: 193 NRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGGNFVPGVSIFGNGYG 252
           NRKLIGAR+F +GY S++ P   +    + RD DGHGTHT STA G+ V G S+ G   G
Sbjct: 191 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 250

Query: 253 TAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDILSVSLGEDAFEFSED 312
           TA+G +P A VA YKVCW     G CF +D+LA  + AI+D V++LS+SLG    ++  D
Sbjct: 251 TARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 310

Query: 313 LMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTIDRLFTTYVALGDNRH 372
            +AIG+F A++ GI V CSAGN+G    S+SNVAPW+ TVGA T+DR F     LG+ ++
Sbjct: 311 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 370

Query: 373 ------FKGESLSSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLDPKKVKGKIVV 432
                 FKGE+L  K+LPF   Y        A +  ++ N  LC+ G+L P+KVKGKIV+
Sbjct: 371 FTGVSLFKGEALPDKLLPF--IY--------AGNASNATNGNLCMTGTLIPEKVKGKIVM 430

Query: 433 CLNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYIDGESINHYIQ 492
           C    ++ V KG     AG VGMIL N   +G++L    HLLPA+ V    G+ I HY+ 
Sbjct: 431 CDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT 490

Query: 493 STITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVNILAAFSGKAS 552
           +   P A I+ + T +G+KP+P++A+FSSRGPN + P+ILKPD+ APGVNILAA++G A 
Sbjct: 491 TDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAG 550

Query: 553 PTDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETEANDL 612
           PT    D RRV +N++SGTSMSCPH+SG+  LLK+++P+WSPAAI+SA+MTTA     D 
Sbjct: 551 PTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDG 610

Query: 613 NPILNSEKEK-ANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGYNKTQMKLFTN 672
            P+L+    K + P  +GAGH+ P  A NPGLIYDL+T+DYL FLC   Y   Q++  + 
Sbjct: 611 KPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR 670

Query: 673 DTSFVC--SKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVGSPSTYVAQVEA-PPGVSV 732
             ++ C  SKS++V DLNYPS ++N     A +  R V +VG   TY  +V +   GV +
Sbjct: 671 -RNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKI 730

Query: 733 SVNPSTLKFTKTGEEKDFKVVLRRVPNNQTEGYMFGKLVWSNGNHHVSSPIFVT 770
           SV P+ L F +  E+K + V      +  +    FG + WS+G H V SP+ ++
Sbjct: 731 SVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmoCh16G010230 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 554.3 bits (1427), Expect = 1.6e-157
Identity = 334/799 (41.80%), Postives = 468/799 (58.57%), Query Frame = 0

Query: 10  LLPFFLFALLQTSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQTHYNLLGSVLGSNEAA 69
           L P      L  S    ++ YIVY G H             + + H++ L SV  S E A
Sbjct: 7   LFPLLFLIPLLASCAEEKQVYIVYFGEHK-----GDKAFHEIEEHHHSYLQSVKESEEDA 66

Query: 70  KESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYEN--KGLKLHTTRSWNFLGV--- 129
           + S+ YSY  +INGFAA L    A  + K  +V+SV+++  +  + HTTRSW F+G+   
Sbjct: 67  RASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEE 126

Query: 130 ENDGGIP-----SDSLWNLSR-------FGESTIIGNIDTGVWPESKSFSDEGYGPIPTR 189
           E D  +P     +D  + + R        G+  I+G +D+GVWPESKSF+D+G GP+P  
Sbjct: 127 ETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKS 186

Query: 190 WKGSCEGGSNF---RCNRKLIGARYFNKGY--ISSVEPRPLNSSYETARDYDGHGTHTLS 249
           WKG C+ G  F    CNRK+IGARY+ KGY           N  + + RD DGHG+HT S
Sbjct: 187 WKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTAS 246

Query: 250 TAGGNFVPGVSIFGN-GYGTAKGGSPNALVAAYKVCW----PPTLDGE-CFMADVLAGFE 309
           TA G  V G S  G    G+A GG+P A +A YK CW       ++G  C   D+LA  +
Sbjct: 247 TAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAID 306

Query: 310 AAISDGVDILSVSLG-EDAFEFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAP 369
            AI+DGV ++S+S+G  + F F++D +A+G+ HAVK  I V  SAGNSG   G++SN+AP
Sbjct: 307 DAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAP 366

Query: 370 WMITVGASTIDRLFTTYVALGDNRHFKGESLSSKILPFKKFYPLIRALDAASDNISSNNS 429
           W+ITVGAST+DR F   + LG+    K +S+++      KF PL+ A +     I+ N +
Sbjct: 367 WIITVGASTLDRAFVGGLVLGNGYTIKTDSITA--FKMDKFAPLVYASNVVVPGIALNET 426

Query: 430 ALCLKGSLDPKKVKGKIVVCLNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHL 489
           + CL  SL P+ V GK+V+CL    S + KG E  +AG  GMIL N   +G+++ +  H 
Sbjct: 427 SQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHF 486

Query: 490 LPASHVSYIDGESINHYIQSTITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILK 549
           +P + V+    + I  YI++   P A+I   KT    + AP M  FSSRGPN V+P+ILK
Sbjct: 487 VPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILK 546

Query: 550 PDITAPGVNILAAFSGKASPTDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWS 609
           PDITAPG+ ILAA+SG  SP+    D+R   YN+ SGTSMSCPH++G + LLK ++PKWS
Sbjct: 547 PDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 606

Query: 610 PAAIKSAIMTTAETEANDLNPILNSEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYL 669
            AAI+SA+MTTA    +   PI ++    ANP A G+GH +P KAA+PGL+YD S + YL
Sbjct: 607 SAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYL 666

Query: 670 NFLCVRGYNKTQMKLFTNDTSFVC-SKSFTVTDLNYPSISMNNLKSEAVEIKRRVKNVG- 729
            + C        + +   D +F C SK     + NYPSI++ NLK + V +KR V NVG 
Sbjct: 667 LYGC-------SVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLK-KTVTVKRTVTNVGT 726

Query: 730 --SPSTYVAQVEAPPGVSVSVNPSTLKFTKTGEEKDFKVVLRRVPN---NQTE--GYMFG 771
             S STY+  V+ P G+SV   P+ L F + G+++ FK+V++ + N   N TE   Y FG
Sbjct: 727 GNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFG 786

BLAST of CmoCh16G010230 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 552.7 bits (1423), Expect = 4.5e-157
Identity = 317/788 (40.23%), Postives = 457/788 (57.99%), Query Frame = 0

Query: 5   NLPPLLLPFFLFALLQ----------TSTIAAQKSYIVYLGSHSHGLNPSALDLQLVTQT 64
           N  PL  P FLF +L           T+ I+ +K+Y++++   +  L P    LQ  +  
Sbjct: 3   NKNPLQKP-FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPL-PYTNHLQWYSSK 62

Query: 65  HYNLLGSVLGSNEAAKESIFYSYNKNINGFAAVLEHKVAEDIAKHPDVISVYENKGLKLH 124
             ++        E     I Y+Y    +G AA L  + AE + +   V++V      +LH
Sbjct: 63  INSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELH 122

Query: 125 TTRSWNFLGVENDGGIPSDSLWNLSRFGESTIIGNIDTGVWPESKSFSDEGYGPIPTRWK 184
           TTRS  FLG+E      S+ +W         ++G +DTG+WPES+SF+D G  P+P  W+
Sbjct: 123 TTRSPTFLGLERQ---ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWR 182

Query: 185 GSCEGGSNF---RCNRKLIGARYFNKGYISSVEPRPLNSSYETARDYDGHGTHTLSTAGG 244
           G+CE G  F    CNRK++GAR F +GY ++         Y++ RD DGHGTHT +T  G
Sbjct: 183 GACETGKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAG 242

Query: 245 NFVPGVSIFGNGYGTAKGGSPNALVAAYKVCWPPTLDGECFMADVLAGFEAAISDGVDIL 304
           + V G ++FG  YGTA+G +  A VAAYKVCW     G CF +D+L+  + A++DGV +L
Sbjct: 243 SPVKGANLFGFAYGTARGMAQKARVAAYKVCWV----GGCFSSDILSAVDQAVADGVQVL 302

Query: 305 SVSLGEDAFEFSEDLMAIGSFHAVKNGITVVCSAGNSGLFEGSVSNVAPWMITVGASTID 364
           S+SLG     +S D ++I +F A++ G+ V CSAGN G    S++NV+PW+ TVGAST+D
Sbjct: 303 SISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMD 362

Query: 365 RLFTTYVALGDNRHFKGESL--SSKILPFKKFYPLIRALDAASDNISSNNSALCLKGSLD 424
           R F   V +G  R FKG SL     +LP  K YPL+     AS   S + ++ CL G+LD
Sbjct: 363 RDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNAS---SPDPTSFCLDGALD 422

Query: 425 PKKVKGKIVVCLNSYDSEVDKGYEAAQAGAVGMILVNDEESGDDLWTAEHLLPASHVSYI 484
            + V GKIV+C       V KG    +AG +GM+L N   +G++L    H+LPA  V   
Sbjct: 423 RRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEK 482

Query: 485 DGESINHYIQSTITPMAYITRVKTELGIKPAPIMASFSSRGPNRVEPSILKPDITAPGVN 544
           +G+ I  Y  ++    A +  + T +GIKP+P++A+FSSRGPN +   ILKPD+ APGVN
Sbjct: 483 EGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVN 542

Query: 545 ILAAFSGKASPTDSPFDKRRVLYNVLSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIM 604
           ILAA++G  +P+    D RRV +N+LSGTSMSCPH+SG+  L+K+ +P WSPAAIKSA+M
Sbjct: 543 ILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALM 602

Query: 605 TTAETEANDLNPILN-SEKEKANPLAYGAGHIQPNKAANPGLIYDLSTQDYLNFLCVRGY 664
           TTA    N   P+ + S    ++P  +GAGHI P +A +PGL+YD+  Q+Y  FLC +  
Sbjct: 603 TTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDL 662

Query: 665 NKTQMKLFTNDTSFVCSKSFTVT--DLNYPSISM---NNLKSEAVEIKRRVKNVGSP-ST 724
           + +Q+K+FT  ++  C  +      +LNYP+IS     N   +A+ ++R V NVG   S+
Sbjct: 663 SPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS 722

Query: 725 YVAQVEAPPGVSVSVNPSTLKFTKTGEEKDFKVVLR-RVPNNQTEGYMFGKLVWSNGNHH 770
           Y   V    G SV+V P TL FT   ++  + V  R R    + E   FG LVW +  H 
Sbjct: 723 YKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE---FGGLVWKSTTHK 775