CmoCh16G009420 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G009420
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	Cmo_Chr16: 5993139 .. 5994730 (+)
RNA-Seq Expression	CmoCh16G009420
Synteny	CmoCh16G009420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAGTATTTACAGACCTTTTTCTTTACCTAAGCAATACGCCATGGCCTTCTTCTCTTCTTCCTTCTACCTCCTCCTTCTCTCCTCCATAGCTCTCTCCGAAGCCGCCGGATGGGTTGGAGTTAACTACGGTCGAATTGCCGACGACCTCCCTCCGCCGGAGAAAGTGGTTAAGCTCCTCAAAGACCAAGGAATCGACCGAATCAAGCTCTACGACACCGACTCCACCGTCCTCACCGCCCTGGCCAACTCCAATATCTCCGTCGTTGTTGCACTTCCCAACGAGCTTCTCTCCTCTGCCGCCGCCGATTCTTCCTTCACTGACAACTGGGTTCAATCCAGCATCTCCCACTTCTATCCCTCCACCAAAATCGACGCCATCGCCGTCGGCAACGAGGTCTTCGTCGACCCAAACAACACCACCAAATTCCTCGTCCCCGCCATGAAAAACGTCTACACCTCCCTCCAAAAATACAACCTCCATAACTCCATTAAAATCTCATCCCCCATTGCCCTAACCGCCCTCGCTTCCTCCTTCCCAACATCCTCCGGTTCCTTCAAACAAGACCTCATCGAGCCAGTCATCAAACCCATGCTCGCCTTCCTCCGCAAAACCCAATCCTATCTAATGATTAACGCCTACCCTTTCTTCGCCTATGCCTCTAACTCCGACAAGATCTCTCTTGACTACGCTCTGTTCCGGGACAACCCCGGAGTTCTCGACTCCGGTAATGGGCTGAAATACTCCAGTCTTTTCGAGGCGCAAATCGACGCCGTTTTTGCTGCCATGTCGGCTCTTAAATACGACGACGTTAAGGTAGTGGTTACGGAAACTGGGTGGCCGTCCAAAGGGGATGAGAAGGACGTGGGAGCAAGTGGGGAGAATGCGGCGTCGTATAACGGCAATTTAGTGAAGAGAGTGCTGACGGGGAGTGGGACCCCTTTGAGACCTAATGACCCACTCAACGTCTTCCTCTTCGCTTTATTCAATGAGAATCAGAAACCGGGCCCCACTTCTGAGAGGAATTACGGTTTGTTTTACCCGAACCAGCAGCGCGTTTATGACATTCCTCTCACACGCGCCGACGTGGAGAGTGGATCTCCAACGATGGTGAATGGTGGTAGCAAGGCTCAAGTACCGTCGTCGAAAGGGCAGACGTGGTGTGTAGCCAACGGGAAGACAGCGGCGGAGAAGCTACAAGCGGGGTTGGATTATGCGTGTGGAGAAGGAGGCGCTGATTGCCGTCCGATTCAACCAGGTTCCACGTGTTACAATCCGAACTCAGTGGAGGCACACGCTTCGTACGCTTTCAATAGCTATTACCAGAAGATGGCTCGTGCGTCTGGCACGTGTGACTTTGGAGGTGCTGCTTACGTCGTTAGTCAACCTCCACGTATGTATCTCTATTAAAATTTATTTGAAGAAATTAATTTTTGATTTAAATAATAATAAAATGATTTTGTTTAAATGGTGCAGAGTACGGAAAATGCGAGTTTCCGACAGGGAACTGAGTTGGGGCAGCTGCGGAAAGTGGTCGAAATCATCATCATCATCATCGGCATTTTAATTTCATTTAGCGAGATAAAACTGAC

mRNA sequence

ATCAGTATTTACAGACCTTTTTCTTTACCTAAGCAATACGCCATGGCCTTCTTCTCTTCTTCCTTCTACCTCCTCCTTCTCTCCTCCATAGCTCTCTCCGAAGCCGCCGGATGGGTTGGAGTTAACTACGGTCGAATTGCCGACGACCTCCCTCCGCCGGAGAAAGTGGTTAAGCTCCTCAAAGACCAAGGAATCGACCGAATCAAGCTCTACGACACCGACTCCACCGTCCTCACCGCCCTGGCCAACTCCAATATCTCCGTCGTTGTTGCACTTCCCAACGAGCTTCTCTCCTCTGCCGCCGCCGATTCTTCCTTCACTGACAACTGGGTTCAATCCAGCATCTCCCACTTCTATCCCTCCACCAAAATCGACGCCATCGCCGTCGGCAACGAGGTCTTCGTCGACCCAAACAACACCACCAAATTCCTCGTCCCCGCCATGAAAAACGTCTACACCTCCCTCCAAAAATACAACCTCCATAACTCCATTAAAATCTCATCCCCCATTGCCCTAACCGCCCTCGCTTCCTCCTTCCCAACATCCTCCGGTTCCTTCAAACAAGACCTCATCGAGCCAGTCATCAAACCCATGCTCGCCTTCCTCCGCAAAACCCAATCCTATCTAATGATTAACGCCTACCCTTTCTTCGCCTATGCCTCTAACTCCGACAAGATCTCTCTTGACTACGCTCTGTTCCGGGACAACCCCGGAGTTCTCGACTCCGGTAATGGGCTGAAATACTCCAGTCTTTTCGAGGCGCAAATCGACGCCGTTTTTGCTGCCATGTCGGCTCTTAAATACGACGACGTTAAGGTAGTGGTTACGGAAACTGGGTGGCCGTCCAAAGGGGATGAGAAGGACGTGGGAGCAAGTGGGGAGAATGCGGCGTCGTATAACGGCAATTTAGTGAAGAGAGTGCTGACGGGGAGTGGGACCCCTTTGAGACCTAATGACCCACTCAACGTCTTCCTCTTCGCTTTATTCAATGAGAATCAGAAACCGGGCCCCACTTCTGAGAGGAATTACGGTTTGTTTTACCCGAACCAGCAGCGCGTTTATGACATTCCTCTCACACGCGCCGACGTGGAGAGTGGATCTCCAACGATGGTGAATGGTGGTAGCAAGGCTCAAGTACCGTCGTCGAAAGGGCAGACGTGGTGTGTAGCCAACGGGAAGACAGCGGCGGAGAAGCTACAAGCGGGGTTGGATTATGCGTGTGGAGAAGGAGGCGCTGATTGCCGTCCGATTCAACCAGGTTCCACGTGTTACAATCCGAACTCAGTGGAGGCACACGCTTCGTACGCTTTCAATAGCTATTACCAGAAGATGGCTCGTGCGTCTGGCACGTGTGACTTTGGAGGTGCTGCTTACGTCGTTAGTCAACCTCCACAGTACGGAAAATGCGAGTTTCCGACAGGGAACTGAGTTGGGGCAGCTGCGGAAAGTGGTCGAAATCATCATCATCATCATCGGCATTTTAATTTCATTTAGCGAGATAAAACTGAC

Coding sequence (CDS)

ATGGCCTTCTTCTCTTCTTCCTTCTACCTCCTCCTTCTCTCCTCCATAGCTCTCTCCGAAGCCGCCGGATGGGTTGGAGTTAACTACGGTCGAATTGCCGACGACCTCCCTCCGCCGGAGAAAGTGGTTAAGCTCCTCAAAGACCAAGGAATCGACCGAATCAAGCTCTACGACACCGACTCCACCGTCCTCACCGCCCTGGCCAACTCCAATATCTCCGTCGTTGTTGCACTTCCCAACGAGCTTCTCTCCTCTGCCGCCGCCGATTCTTCCTTCACTGACAACTGGGTTCAATCCAGCATCTCCCACTTCTATCCCTCCACCAAAATCGACGCCATCGCCGTCGGCAACGAGGTCTTCGTCGACCCAAACAACACCACCAAATTCCTCGTCCCCGCCATGAAAAACGTCTACACCTCCCTCCAAAAATACAACCTCCATAACTCCATTAAAATCTCATCCCCCATTGCCCTAACCGCCCTCGCTTCCTCCTTCCCAACATCCTCCGGTTCCTTCAAACAAGACCTCATCGAGCCAGTCATCAAACCCATGCTCGCCTTCCTCCGCAAAACCCAATCCTATCTAATGATTAACGCCTACCCTTTCTTCGCCTATGCCTCTAACTCCGACAAGATCTCTCTTGACTACGCTCTGTTCCGGGACAACCCCGGAGTTCTCGACTCCGGTAATGGGCTGAAATACTCCAGTCTTTTCGAGGCGCAAATCGACGCCGTTTTTGCTGCCATGTCGGCTCTTAAATACGACGACGTTAAGGTAGTGGTTACGGAAACTGGGTGGCCGTCCAAAGGGGATGAGAAGGACGTGGGAGCAAGTGGGGAGAATGCGGCGTCGTATAACGGCAATTTAGTGAAGAGAGTGCTGACGGGGAGTGGGACCCCTTTGAGACCTAATGACCCACTCAACGTCTTCCTCTTCGCTTTATTCAATGAGAATCAGAAACCGGGCCCCACTTCTGAGAGGAATTACGGTTTGTTTTACCCGAACCAGCAGCGCGTTTATGACATTCCTCTCACACGCGCCGACGTGGAGAGTGGATCTCCAACGATGGTGAATGGTGGTAGCAAGGCTCAAGTACCGTCGTCGAAAGGGCAGACGTGGTGTGTAGCCAACGGGAAGACAGCGGCGGAGAAGCTACAAGCGGGGTTGGATTATGCGTGTGGAGAAGGAGGCGCTGATTGCCGTCCGATTCAACCAGGTTCCACGTGTTACAATCCGAACTCAGTGGAGGCACACGCTTCGTACGCTTTCAATAGCTATTACCAGAAGATGGCTCGTGCGTCTGGCACGTGTGACTTTGGAGGTGCTGCTTACGTCGTTAGTCAACCTCCACAGTACGGAAAATGCGAGTTTCCGACAGGGAACTGA

Protein sequence

MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN

Homology

[Close]

BLAST of CmoCh16G009420 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 8.3e-103
Identity = 192/458 (41.92%), Postives = 287/458 (62.66%), Query Frame = 0


Query: 4   FSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTV 63
           FS S  LLLL         G VGV YGR ADDLP P KVV+L++   I  +++YD +S V
Sbjct: 9   FSISILLLLLDCC----YGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQV 68

Query: 64  LTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDP 123
           L A  N++I +++ +PN  L++ +   S  D W+++S+  +YP+TKI  I VG E   DP
Sbjct: 69  LKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDP 128

Query: 124 N-NTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIK 183
           + N + F+VPAM+NV T+L+K  L   IK+S+ ++L  L+ SFP S+G+F        ++
Sbjct: 129 HINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAY-FLR 188

Query: 184 PMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQI 243
           PML FL + +S  MI+ YP++AY  + + +SLDY LF  +  V+D   GL Y ++F+AQ+
Sbjct: 189 PMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQV 248

Query: 244 DAVFAAMSALKYDDVKVVVTETGWPSKGDEKD-VGASGENAASYNGNLVKRVLTGSGTPL 303
           DA++ A++AL +  +K++VTETGWP+KG  K+   AS +NA +YN N+++ V+T  GTP 
Sbjct: 249 DALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPA 308

Query: 304 RPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGS 363
           +P + +NV++F+LFNEN+K G  SERN+GLFYP+Q  VY +  T      G     +  S
Sbjct: 309 KPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFT------GKSNGFHSNS 368

Query: 364 KAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASY 423
                S    +WC+A+ K +   L+  LD+ACG G  DC  IQP   C+ P+++ +HAS+
Sbjct: 369 SGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASF 428

Query: 424 AFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPT 460
            FNSY+Q+       C FGGA   V++ P Y KC + T
Sbjct: 429 VFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYIT 455

[Close]

BLAST of CmoCh16G009420 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.4e-102
Identity = 190/451 (42.13%), Postives = 286/451 (63.41%), Query Frame = 0


Query: 22  AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTALANSNISVVVALPNE 81
           A  +G+ YGR AD+LP P +V +L++   I  +++YD +  VL A AN+ I +++ +PN 
Sbjct: 24  ASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNA 83

Query: 82  LLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNNTTKFLVPAMKNVYTSL 141
            L + A   S  D W+ ++I  +YPSTKI +I+VG EV   P+N T  ++PAM+N++T+L
Sbjct: 84  DLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTAL 143

Query: 142 QKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLAFLRKTQSYLMINAYP 201
           +K  L   IKISS  +L  L+ SFP SS SF +      +KPML FL + +S  MI+ YP
Sbjct: 144 KKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLEFLVENESPFMIDLYP 203

Query: 202 FFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAVFAAMSALKYDDVKVVV 261
           ++AY  +++K+ L+YALF  +  V+D   GL YS++F+AQ+DA++ A++A+ +  VKV+V
Sbjct: 204 YYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMV 263

Query: 262 TETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPNDPLNVFLFALFNENQKP 321
           TE+GWPSKG  K+  A+ ENA +YN NL++ V+   GTP +P + ++V+LF+LFNEN+KP
Sbjct: 264 TESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKP 323

Query: 322 GPTSERNYGLFYPNQQRVYDIPLTRADVESGSPT--------------MVNGGSKAQVPS 381
           G  SERN+G+FY N   VY +  T  +    SPT              ++NG S   +  
Sbjct: 324 GIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGG 383

Query: 382 SKG---QTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAFN 441
             G   + WC+A+ + +  +LQ  LD+ACG G  DC  +QP   C+ P++V +HASYAFN
Sbjct: 384 GGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFN 443

Query: 442 SYYQKMARASGTCDFGGAAYVVSQPPQYGKC 456
           +YYQ+   +S  C F GA+  V + P YG C
Sbjct: 444 TYYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

[Close]

BLAST of CmoCh16G009420 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 365.9 bits (938), Expect = 6.6e-100
Identity = 195/457 (42.67%), Postives = 282/457 (61.71%), Query Frame = 0


Query: 8   FYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTAL 67
           F L+ LS    S A  ++GVNYG++AD+LPPP + VKLL+   I +++LY  D  ++ AL
Sbjct: 9   FLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKAL 68

Query: 68  ANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFV--DPNN 127
           A + + +V+   N  + S A+D +    W+ S++  FYP++KI  I VGNE+ +  DPN 
Sbjct: 69  AGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNL 128

Query: 128 TTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPML 187
             + L+PAM+NV  +L+  +L   IK+S+  ++T L SS P SSGSF     +  +K +L
Sbjct: 129 VNQ-LLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAG-YQTGLKGIL 188

Query: 188 AFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAV 247
            FL  T S   IN YPFFAY S+    +L + LF  N G +DS  G+KY+++F+AQ+DAV
Sbjct: 189 QFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAV 248

Query: 248 FAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPND 307
            +A+ ++ ++ V++VV ETGW S+GD  +VGAS +NA +YNGNL+  + +  GTPL P  
Sbjct: 249 HSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGK 308

Query: 308 PLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQV 367
           P++ ++FAL++EN KPGP+SER +GLF  +   VYD+ L ++             S +Q 
Sbjct: 309 PVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKS------------SSSSQT 368

Query: 368 PSSK--GQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAF 427
           PS K     WCV       E+LQA LD+ACG  G DC  IQPG  C+ PN+V +HA+YA 
Sbjct: 369 PSGKVTSSGWCVPKKGATNEELQASLDWACGH-GIDCGAIQPGGACFEPNNVVSHAAYAM 428

Query: 428 NSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 461
           N Y+QK  +    CDF   A V SQ P Y  C +P G
Sbjct: 429 NMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

[Close]

BLAST of CmoCh16G009420 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 351.3 bits (900), Expect = 1.7e-95
Identity = 183/453 (40.40%), Postives = 274/453 (60.49%), Query Frame = 0


Query: 9   YLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTALA 68
           +L L +S ALS+ +  +GVN G    ++P P +VV LLK Q I+R++LYD D ++L A A
Sbjct: 8   FLFLFASSALSQDS-LIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFA 67

Query: 69  NSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNNTTK 128
           ++ + V++++PN+ L   +  ++   NWV  +++ +YP+T I  IAVG+EV     N   
Sbjct: 68  HTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAAS 127

Query: 129 FLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLAFL 188
            LV A+K +  +L   NL   IK+S+P + T +  SFP S   F +   +PVI P+L FL
Sbjct: 128 VLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTW-DPVIVPLLKFL 187

Query: 189 RKTQSYLMINAYPFFAYASNSDKISLDYALFRD---NPGVLDSGNGLKYSSLFEAQIDAV 248
           + T S L++N YP+F Y  ++  I LDYALF+    N   +D+   L Y+++F+A +DA 
Sbjct: 188 QSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAA 247

Query: 249 FAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPND 308
           + AMS L + ++ +VVTE+GWPSKG   +  A+ ENA +YN NL++ V+  +GTP  P  
Sbjct: 248 YFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGT 307

Query: 309 PLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQV 368
            +  +++ L+NE+ +PGP SE+N+GLFY N   VY + L              G      
Sbjct: 308 AVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLA-------------GAGAILA 367

Query: 369 PSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAFNS 428
             +  QT+C+A  K   + LQA LD+ACG G  DC  +  G +CY P+ V AH++YAFN+
Sbjct: 368 NDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNA 427

Query: 429 YYQKMARASGTCDFGGAAYVVSQPPQYGKCEFP 459
           YYQKM +ASG+CDF G A V +  P  G C FP
Sbjct: 428 YYQKMGKASGSCDFKGVATVTTTDPSRGTCVFP 445

[Close]

BLAST of CmoCh16G009420 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 3.0e-92
Identity = 182/451 (40.35%), Postives = 270/451 (59.87%), Query Frame = 0


Query: 10  LLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTALAN 69
           LLLLS +A S AA ++GVN G    ++PPP  +V LLK Q I  ++LYD +S +L A AN
Sbjct: 12  LLLLSILACSNAA-FIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFAN 71

Query: 70  SNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNNTTKF 129
           ++I V+V + NE +       S    WV  +++ + PST I AIAVG+EV     +    
Sbjct: 72  TSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPI 131

Query: 130 LVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLAFLR 189
           L  A+ N++ +L   NL+  +K+SSP+++  +   FP S+ +F        +  +L FL+
Sbjct: 132 LASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSW-NTTVYQLLQFLK 191

Query: 190 KTQSYLMINAYPFFAYASNSDKISLDYALFRD-NP--GVLDSGNGLKYSSLFEAQIDAVF 249
            T S+ M+NAYP++ Y + +    LDYALF+  +P   ++D    L Y+S+F+A +DA +
Sbjct: 192 NTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAY 251

Query: 250 AAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPNDP 309
            +M AL +  + VVVTETGWPS G   +  A+  NA ++N NL+KRVL  SG P +P+ P
Sbjct: 252 YSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIP 311

Query: 310 LNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQVP 369
           +N +++ L+NE+++ GP SERN+G+ +PN   VY + L+       S   +NG S     
Sbjct: 312 INTYIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLS----GGSSSAALNGSS----- 371

Query: 370 SSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAFNSY 429
                 +CVA      +KL  GL++ACG+G A+C  IQPG  CY PN V++HAS+AFN Y
Sbjct: 372 -----MFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDY 431

Query: 430 YQKMARASGTCDFGGAAYVVSQPPQYGKCEF 458
           YQKM  A GTCDF G A   ++ P Y  C +
Sbjct: 432 YQKMKSAGGTCDFDGTAITTTRDPSYRTCAY 446

[Close]

BLAST of CmoCh16G009420 vs. ExPASy TrEMBL
Match: A0A6J1EW84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438639 PE=3 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 7.0e-262
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60
           MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD
Sbjct: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60

Query: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120
           STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF
Sbjct: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120

Query: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180
           VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV
Sbjct: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180

Query: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240
           IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA
Sbjct: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240

Query: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300
           QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP
Sbjct: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300

Query: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360
           LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG
Sbjct: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360

Query: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420
           SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS
Sbjct: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420

Query: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN
Sbjct: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 461

[Close]

BLAST of CmoCh16G009420 vs. ExPASy TrEMBL
Match: A0A6J1HJA3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465026 PE=3 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.3e-252
Identity = 446/462 (96.54%), Postives = 456/462 (98.70%), Query Frame = 0


Query: 1   MAFFSSSFY-LLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDT 60
           MAFFSSSFY LLLLSSIALSEAAGWVGVNYGRIADDLP P+KVVKLLKDQGIDRIKLYDT
Sbjct: 1   MAFFSSSFYLLLLLSSIALSEAAGWVGVNYGRIADDLPSPDKVVKLLKDQGIDRIKLYDT 60

Query: 61  DSTVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEV 120
           DS VLTALANSNISVVVALPNELLSSAAADSSFTD+WVQSSISHFYPSTKIDAIAVGNEV
Sbjct: 61  DSAVLTALANSNISVVVALPNELLSSAAADSSFTDSWVQSSISHFYPSTKIDAIAVGNEV 120

Query: 121 FVDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEP 180
           FVDPNNTTKFLVPAMKNVYTSLQKYNLH+SIKISSPIALTALASSFPTSSGSFKQDLIEP
Sbjct: 121 FVDPNNTTKFLVPAMKNVYTSLQKYNLHSSIKISSPIALTALASSFPTSSGSFKQDLIEP 180

Query: 181 VIKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFE 240
           VIKPMLAFLR+TQSYLM+NAYPFFAYASNSDKISLDYALF+DNPGV+DSGNGLKYSSLFE
Sbjct: 181 VIKPMLAFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFQDNPGVVDSGNGLKYSSLFE 240

Query: 241 AQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGT 300
           AQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGAS ENAASYNGNLVKRVLTGSGT
Sbjct: 241 AQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASEENAASYNGNLVKRVLTGSGT 300

Query: 301 PLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNG 360
           PLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVES +PTMVNG
Sbjct: 301 PLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESEAPTMVNG 360

Query: 361 GSKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 420
           GSKAQVPSSKGQTWCVANGKTAAE+LQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA
Sbjct: 361 GSKAQVPSSKGQTWCVANGKTAAERLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 420

Query: 421 SYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           SYAFNSYYQKM RASGTC+FGGAAYVVSQPPQYGKCEFPTGN
Sbjct: 421 SYAFNSYYQKMGRASGTCEFGGAAYVVSQPPQYGKCEFPTGN 462

[Close]

BLAST of CmoCh16G009420 vs. ExPASy TrEMBL
Match: A0A6J1KYS6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498785 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.5e-211
Identity = 373/460 (81.09%), Postives = 414/460 (90.00%), Query Frame = 0


Query: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60
           M FF   F  LL S+ A  E  G +GVNYGRIAD+LPPP+KVV LLK QGIDRIK+YDTD
Sbjct: 3   MPFF--PFLYLLFSATAHGE-PGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTD 62

Query: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120
           + VLTAL+NSNIS+VVALPN LLSSAAADSSFTDNWVQS IS++YP+TKI+A+AVGNEVF
Sbjct: 63  AAVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVF 122

Query: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180
           VDPNNTT FL+PAMKNVY+SLQKYNLH+SIK+SSPIALTALASS+PTSSGSFK DLIEPV
Sbjct: 123 VDPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPV 182

Query: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240
           IKPML FL +T+SYLM+NAYPFFAYA+NSD+ISLDYALFR+N GV+D GNGLKY SLFEA
Sbjct: 183 IKPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEA 242

Query: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300
           Q+DAV+AAM ALKYDD+KVVVTETGWPS G+EK+VGAS ENAASYNGNLV+RVLTG GTP
Sbjct: 243 QVDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTP 302

Query: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360
           LRP DPL+VFLFALFNENQKPGPTSERNYGLFYPN+++VY+IP T AD+ S +PT VN G
Sbjct: 303 LRPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVN-G 362

Query: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420
           SKAQVP SKG TWCVANG  AAEKL+ GLDYACGEGGADCR IQPGSTCYNPNS+EAHAS
Sbjct: 363 SKAQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHAS 422

Query: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 461
           YAFNSYYQKM RASGTC FGG AYVV+QPPQYG CEFPTG
Sbjct: 423 YAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTG 458

[Close]

BLAST of CmoCh16G009420 vs. ExPASy TrEMBL
Match: A0A6J1GIP4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454517 PE=3 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.6e-211
Identity = 372/460 (80.87%), Postives = 412/460 (89.57%), Query Frame = 0


Query: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60
           MA   S F  LL S+ AL E  G +GVNYGRIAD+LPPP+KVV LLK QGIDRIK+YDTD
Sbjct: 1   MAIAFSPFLFLLFSATALGE-PGLIGVNYGRIADNLPPPDKVVGLLKQQGIDRIKVYDTD 60

Query: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120
           + VLTAL+NSNIS+VVAL N+LLSSAAADSSFTDNWVQS IS++YP+TKI+A+AVGNEVF
Sbjct: 61  AAVLTALSNSNISIVVALSNDLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVF 120

Query: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180
           VDPNNTT FL+PAM+NVY+SLQKYNLH+SIK+SSPIALTALASS+PTSSGSFK DLIEPV
Sbjct: 121 VDPNNTTNFLIPAMRNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPV 180

Query: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240
           IKPML FL +T+SYLM+NAYPFFAYA+NSD+ISLDYALFR+N GV+D GNGLKY SLFEA
Sbjct: 181 IKPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEA 240

Query: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300
           Q+DAV+AAM ALKYDDVKVVVTETGWPS G+EK+VGAS ENAASYNGNLV+RVLTG GTP
Sbjct: 241 QVDAVYAAMMALKYDDVKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGKGTP 300

Query: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360
           LRP DPL+VFLFALFNENQKPGPTSERNYGLFYPN++ VY IP T AD+ S +PT VN G
Sbjct: 301 LRPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEEVYQIPFTLADMASETPTPVN-G 360

Query: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420
           SKAQVP SKG TWCVANG  AAEKL+ GLDYACGEGGADC  IQPGSTCYNPNS+EAHAS
Sbjct: 361 SKAQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCGAIQPGSTCYNPNSLEAHAS 420

Query: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 461
           YAFNSYYQKM RASGTC FGG AYVV+QPPQYG CEFPTG
Sbjct: 421 YAFNSYYQKMGRASGTCYFGGVAYVVAQPPQYGNCEFPTG 458

[Close]

BLAST of CmoCh16G009420 vs. ExPASy TrEMBL
Match: A0A0A0L687 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G073920 PE=3 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 1.1e-206
Identity = 362/461 (78.52%), Postives = 409/461 (88.72%), Query Frame = 0


Query: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60
           MAFFSS  + LL SS  LS AAGWVGVNYGRIADDLP P+ VVKLLK  GIDRIKL+DT+
Sbjct: 1   MAFFSSLSFFLLFSSF-LSHAAGWVGVNYGRIADDLPSPDDVVKLLKTHGIDRIKLFDTE 60

Query: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120
           STVLTAL+NSNISVVV+LPNE LSSAA+D SFTDNWVQS+ISHFYPSTKIDAIAVGNEVF
Sbjct: 61  STVLTALSNSNISVVVSLPNEYLSSAASDPSFTDNWVQSNISHFYPSTKIDAIAVGNEVF 120

Query: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180
           VDPNNTT FLVPAMKNVY SLQK+NLH +IK+S+P+A +ALASS+PTSSGSFK DLIEPV
Sbjct: 121 VDPNNTTNFLVPAMKNVYASLQKFNLHTNIKVSTPLAFSALASSYPTSSGSFKPDLIEPV 180

Query: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240
           +KPML  +R++QS+LM+NAYPFFAY  N+DKIS+DYALFR+N GV+DSGNGLKYS+L EA
Sbjct: 181 MKPMLDLIRQSQSHLMVNAYPFFAYIGNADKISIDYALFRENAGVIDSGNGLKYSNLLEA 240

Query: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300
           Q+DAVFAA+SA+K++D+ VVVTETGWPSKGDE ++GAS ENAA+YNGNLVKRVLTGSGTP
Sbjct: 241 QVDAVFAALSAIKFEDIPVVVTETGWPSKGDENEIGASIENAAAYNGNLVKRVLTGSGTP 300

Query: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVES--GSPTMVN 360
           LRP +PLN +LFALFNEN+K GPTSERNYGLFYPN+++VY+IPLT ADVE   GS   VN
Sbjct: 301 LRPKEPLNAYLFALFNENKKQGPTSERNYGLFYPNEEKVYEIPLTSADVEGGVGSTPAVN 360

Query: 361 GGSKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAH 420
           G    +     GQTWCVANGK A EKLQAGLDYACGEGGADC  IQPG+TCYNPNS+EAH
Sbjct: 361 GSKVIK----NGQTWCVANGKVAPEKLQAGLDYACGEGGADCSSIQPGATCYNPNSLEAH 420

Query: 421 ASYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPT 460
           ASYAFNSYYQKM RA GTCDFGGAAYVV+QPPQYG+CEFPT
Sbjct: 421 ASYAFNSYYQKMKRAVGTCDFGGAAYVVTQPPQYGQCEFPT 456

[Close]

BLAST of CmoCh16G009420 vs. NCBI nr
Match: XP_022932287.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata])

HSP 1 Score: 912.5 bits (2357), Expect = 1.4e-261
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60
           MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD
Sbjct: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60

Query: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120
           STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF
Sbjct: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120

Query: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180
           VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV
Sbjct: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180

Query: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240
           IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA
Sbjct: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240

Query: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300
           QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP
Sbjct: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300

Query: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360
           LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG
Sbjct: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360

Query: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420
           SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS
Sbjct: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420

Query: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN
Sbjct: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 461

[Close]

BLAST of CmoCh16G009420 vs. NCBI nr
Match: KAG6577464.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 910.6 bits (2352), Expect = 5.5e-261
Identity = 459/461 (99.57%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60
           MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD
Sbjct: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60

Query: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120
           STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF
Sbjct: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120

Query: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180
           VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV
Sbjct: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180

Query: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240
           IKPMLAFLR+TQSYLM+NAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA
Sbjct: 181 IKPMLAFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240

Query: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300
           QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP
Sbjct: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300

Query: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360
           LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG
Sbjct: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360

Query: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420
           SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS
Sbjct: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420

Query: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN
Sbjct: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 461

[Close]

BLAST of CmoCh16G009420 vs. NCBI nr
Match: KAG7015534.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 908.7 bits (2347), Expect = 2.1e-260
Identity = 458/461 (99.35%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60
           MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD
Sbjct: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60

Query: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120
           STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF
Sbjct: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120

Query: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180
           VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV
Sbjct: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180

Query: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240
           IKPMLAFLR+TQSYLM+NAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA
Sbjct: 181 IKPMLAFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240

Query: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300
           QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP
Sbjct: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300

Query: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360
           LRPNDPL+VFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG
Sbjct: 301 LRPNDPLDVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360

Query: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420
           SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS
Sbjct: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420

Query: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN
Sbjct: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 461

[Close]

BLAST of CmoCh16G009420 vs. NCBI nr
Match: XP_023551952.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 883.6 bits (2282), Expect = 7.2e-253
Identity = 446/458 (97.38%), Postives = 451/458 (98.47%), Query Frame = 0


Query: 8   FYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTAL 67
           FYLLLLSSIALS+AAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDS VLTAL
Sbjct: 4   FYLLLLSSIALSDAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSAVLTAL 63

Query: 68  ANSNISVVVALPNELLSS----AAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDP 127
           ANSNISVVVALPNELLSS    AAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDP
Sbjct: 64  ANSNISVVVALPNELLSSAAAAAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDP 123

Query: 128 NNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKP 187
           NNTTKFL+PAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKP
Sbjct: 124 NNTTKFLIPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKP 183

Query: 188 MLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQID 247
           MLAFLR+TQSYLM+NAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQID
Sbjct: 184 MLAFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQID 243

Query: 248 AVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRP 307
           AVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTG GTPLRP
Sbjct: 244 AVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGRGTPLRP 303

Query: 308 NDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKA 367
           ND LNVFLFALFNEN+KPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKA
Sbjct: 304 NDSLNVFLFALFNENRKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKA 363

Query: 368 QVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAF 427
           QVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAF
Sbjct: 364 QVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAF 423

Query: 428 NSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           NSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN
Sbjct: 424 NSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 461

[Close]

BLAST of CmoCh16G009420 vs. NCBI nr
Match: XP_022965047.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima])

HSP 1 Score: 881.7 bits (2277), Expect = 2.7e-252
Identity = 446/462 (96.54%), Postives = 456/462 (98.70%), Query Frame = 0


Query: 1   MAFFSSSFY-LLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDT 60
           MAFFSSSFY LLLLSSIALSEAAGWVGVNYGRIADDLP P+KVVKLLKDQGIDRIKLYDT
Sbjct: 1   MAFFSSSFYLLLLLSSIALSEAAGWVGVNYGRIADDLPSPDKVVKLLKDQGIDRIKLYDT 60

Query: 61  DSTVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEV 120
           DS VLTALANSNISVVVALPNELLSSAAADSSFTD+WVQSSISHFYPSTKIDAIAVGNEV
Sbjct: 61  DSAVLTALANSNISVVVALPNELLSSAAADSSFTDSWVQSSISHFYPSTKIDAIAVGNEV 120

Query: 121 FVDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEP 180
           FVDPNNTTKFLVPAMKNVYTSLQKYNLH+SIKISSPIALTALASSFPTSSGSFKQDLIEP
Sbjct: 121 FVDPNNTTKFLVPAMKNVYTSLQKYNLHSSIKISSPIALTALASSFPTSSGSFKQDLIEP 180

Query: 181 VIKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFE 240
           VIKPMLAFLR+TQSYLM+NAYPFFAYASNSDKISLDYALF+DNPGV+DSGNGLKYSSLFE
Sbjct: 181 VIKPMLAFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFQDNPGVVDSGNGLKYSSLFE 240

Query: 241 AQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGT 300
           AQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGAS ENAASYNGNLVKRVLTGSGT
Sbjct: 241 AQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASEENAASYNGNLVKRVLTGSGT 300

Query: 301 PLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNG 360
           PLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVES +PTMVNG
Sbjct: 301 PLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESEAPTMVNG 360

Query: 361 GSKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 420
           GSKAQVPSSKGQTWCVANGKTAAE+LQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA
Sbjct: 361 GSKAQVPSSKGQTWCVANGKTAAERLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 420

Query: 421 SYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           SYAFNSYYQKM RASGTC+FGGAAYVVSQPPQYGKCEFPTGN
Sbjct: 421 SYAFNSYYQKMGRASGTCEFGGAAYVVSQPPQYGKCEFPTGN 462

[Close]

BLAST of CmoCh16G009420 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 657.1 bits (1694), Expect = 1.0e-188
Identity = 336/466 (72.10%), Postives = 390/466 (83.69%), Query Frame = 0


Query: 1   MAFFSSSFYLL-LLSSIALSEA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYD 60
           MA F  S  +L   S+I  + A +G +GVNYGRIAD+LP PEKVV+LLK QGI+RIKLYD
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 61  TDSTVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNE 120
           T++TVLTALANS I VVV+LPNE L+SAAAD S+TD WVQ +I  + P+T I+AIAVGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 121 VFVDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIE 180
           VFVDP NTT +LVPAMKNV +SL K+NL  SIKISSPIAL+ALASS+P S+GSFK +LIE
Sbjct: 121 VFVDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIE 180

Query: 181 PVIKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLF 240
           PVIKPML  LRKT S+LM+NAYPFFAYA+N+DKISLDYALF++N G +DSGNGLKY+SL 
Sbjct: 181 PVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLL 240

Query: 241 EAQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSG 300
           +AQIDAVFAAMSA+ ++DVK+VVTETGWPS GDE ++GA   NAA+YNG LVKRVLTG+G
Sbjct: 241 DAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNG 300

Query: 301 TPLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVN 360
           TPL+P +PLNV+LFALFNENQK GPTSERNYGLFYPN+ +VYD+ L      +G  T VN
Sbjct: 301 TPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSL------NGKSTPVN 360

Query: 361 GGSKAQV---PSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSV 420
              +  V   PS  GQTWCVANGKT  EKLQ GLDYACGEGGADCRPIQPG+TCYNP S+
Sbjct: 361 DNKEKVVPVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESL 420

Query: 421 EAHASYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           EAHASYAFNSYYQK AR  GTC+FGGAAYVVSQPP+YGKCEFPTG+
Sbjct: 421 EAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPTGH 460

[Close]

BLAST of CmoCh16G009420 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 634.0 bits (1634), Expect = 9.2e-182
Identity = 327/455 (71.87%), Postives = 379/455 (83.30%), Query Frame = 0


Query: 1   MAFFSSSFYLL-LLSSIALSEA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYD 60
           MA F  S  +L   S+I  + A +G +GVNYGRIAD+LP PEKVV+LLK QGI+RIKLYD
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 61  TDSTVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNE 120
           T++TVLTALANS I VVV+LPNE L+SAAAD S+TD WVQ +I  + P+T I+AIAVGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 121 VFVDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIE 180
           VFVDP NTT +LVPAMKNV +SL K+NL  SIKISSPIAL+ALASS+P S+GSFK +LIE
Sbjct: 121 VFVDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIE 180

Query: 181 PVIKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLF 240
           PVIKPML  LRKT S+LM+NAYPFFAYA+N+DKISLDYALF++N G +DSGNGLKY+SL 
Sbjct: 181 PVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLL 240

Query: 241 EAQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSG 300
           +AQIDAVFAAMSA+ ++DVK+VVTETGWPS GDE ++GA   NAA+YNG LVKRVLTG+G
Sbjct: 241 DAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNG 300

Query: 301 TPLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVN 360
           TPL+P +PLNV+LFALFNENQK GPTSERNYGLFYPN+ +VYD+ L      +G  T VN
Sbjct: 301 TPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSL------NGKSTPVN 360

Query: 361 GGSKAQV---PSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSV 420
              +  V   PS  GQTWCVANGKT  EKLQ GLDYACGEGGADCRPIQPG+TCYNP S+
Sbjct: 361 DNKEKVVPVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESL 420

Query: 421 EAHASYAFNSYYQKMARASGTCDFGGAAYVVSQPP 451
           EAHASYAFNSYYQK AR  GTC+FGGAAYVVSQPP
Sbjct: 421 EAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPP 449

[Close]

BLAST of CmoCh16G009420 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 631.7 bits (1628), Expect = 4.6e-181
Identity = 316/455 (69.45%), Postives = 385/455 (84.62%), Query Frame = 0


Query: 9   YLLLLSSIALSEA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTAL 68
           Y L+LS ++  +A +G VGVNYGRIA++LP PEKVV LLK QGI+RIK++DTD  VLTAL
Sbjct: 8   YFLILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVLTAL 67

Query: 69  ANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNNTT 128
           ANS I V+VALPNELLSSAA+  SF DNW+++ I  ++P+T+I+AIAVGNEVFVDP   T
Sbjct: 68  ANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP-TIT 127

Query: 129 KFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLAF 188
            +LV AMKN++TSL KY L  +IKISSPIAL+ALA+S+P SSGSFK +LIEPV+KPMLA 
Sbjct: 128 PYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPMLAL 187

Query: 189 LRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAVFA 248
           L++T SYLM+NAYPFFAYA+N+DKISLDYALF++N G +DSG GLKY+SLF+AQIDAV+A
Sbjct: 188 LQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDAVYA 247

Query: 249 AMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPNDPL 308
           A+SA+ +  VKV+VTETGWPS GDE ++GAS  NAA+YN  LVKRVLTG GTPLRP +PL
Sbjct: 248 ALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPTEPL 307

Query: 309 NVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQVP- 368
           NV+LFALFNENQKPGPTSERNYGLFYPN+ +VY++P T+      S T VN G++ +VP 
Sbjct: 308 NVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKK-----STTPVN-GNRGKVPV 367

Query: 369 SSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASYAFNSY 428
           + +G TWCV+NG+ A EKLQ  LDYACGEGGADCRPIQPG+TCY+P S+EAHASYAFNSY
Sbjct: 368 THEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLEAHASYAFNSY 427

Query: 429 YQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTGN 462
           YQK +R  GTC FGGAA+VV+QPP+YGKCEFPTG+
Sbjct: 428 YQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPTGH 455

[Close]

BLAST of CmoCh16G009420 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 602.8 bits (1553), Expect = 2.3e-172
Identity = 299/464 (64.44%), Postives = 373/464 (80.39%), Query Frame = 0


Query: 8   FYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLTAL 67
           F LLLLS  +LS+ AG +GVNYGRI+D+LP   KVV+LLK QGI R+K++D D +VL AL
Sbjct: 9   FLLLLLSPFSLSD-AGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKAL 68

Query: 68  ANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNNTT 127
           + S I V V LPNELL SAA  +SF  +WV+ +++ ++PST+I++IAVGNEVFVD +NTT
Sbjct: 69  SGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNTT 128

Query: 128 KFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLAF 187
            FL+PAM+N++ +L  +NLH+ IKISSP+AL+AL +S+P+SSGSF+ +LI+ VIKPML F
Sbjct: 129 SFLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPMLDF 188

Query: 188 LRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAVFA 247
           LR+T S LMIN YPFFAY  NSD I LDYAL R+NPG++DSGNGL+Y +LF+AQIDAVFA
Sbjct: 189 LRETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDAQIDAVFA 248

Query: 248 AMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPNDPL 307
           AMSALKYDD++++VTETGWPSKGDE +VGA+  NAASYNGNL++R+LT  GTPLRP   L
Sbjct: 249 AMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTPLRPKADL 308

Query: 308 NVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVE---SGSPTMVNGGSKAQ 367
            V+LFALFNEN+K GPTSERNYGLF+P++++VYDIP T   ++    G  T V GG +  
Sbjct: 309 TVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGHTPVTGGDQVT 368

Query: 368 VP--------SSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVE 427
            P        S  G TWCVANG    E+LQ GLDYACGEGGADCRPIQPG+ CY+P+++E
Sbjct: 369 KPPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPDTLE 428

Query: 428 AHASYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 461
           AHAS+AFNSYYQK  RA G+C FGGAAYVVSQPP+YG+CEFPTG
Sbjct: 429 AHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPTG 471

[Close]

BLAST of CmoCh16G009420 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 375.6 bits (963), Expect = 5.9e-104
Identity = 192/458 (41.92%), Postives = 287/458 (62.66%), Query Frame = 0


Query: 4   FSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTV 63
           FS S  LLLL         G VGV YGR ADDLP P KVV+L++   I  +++YD +S V
Sbjct: 9   FSISILLLLLDCC----YGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQV 68

Query: 64  LTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDP 123
           L A  N++I +++ +PN  L++ +   S  D W+++S+  +YP+TKI  I VG E   DP
Sbjct: 69  LKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDP 128

Query: 124 N-NTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIK 183
           + N + F+VPAM+NV T+L+K  L   IK+S+ ++L  L+ SFP S+G+F        ++
Sbjct: 129 HINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAY-FLR 188

Query: 184 PMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQI 243
           PML FL + +S  MI+ YP++AY  + + +SLDY LF  +  V+D   GL Y ++F+AQ+
Sbjct: 189 PMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQV 248

Query: 244 DAVFAAMSALKYDDVKVVVTETGWPSKGDEKD-VGASGENAASYNGNLVKRVLTGSGTPL 303
           DA++ A++AL +  +K++VTETGWP+KG  K+   AS +NA +YN N+++ V+T  GTP 
Sbjct: 249 DALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPA 308

Query: 304 RPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGS 363
           +P + +NV++F+LFNEN+K G  SERN+GLFYP+Q  VY +  T      G     +  S
Sbjct: 309 KPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFT------GKSNGFHSNS 368

Query: 364 KAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHASY 423
                S    +WC+A+ K +   L+  LD+ACG G  DC  IQP   C+ P+++ +HAS+
Sbjct: 369 SGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASF 428

Query: 424 AFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPT 460
            FNSY+Q+       C FGGA   V++ P Y KC + T
Sbjct: 429 VFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYIT 455

The following BLAST results are available for this feature:

BLAST of CmoCh16G009420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJU9	8.3e-103	41.92	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q8VYE5	1.4e-102	42.13	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9M069	6.6e-100	42.67	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q9ZU91	1.7e-95	40.40	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q94CD8	3.0e-92	40.35	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]

BLAST of CmoCh16G009420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EW84	7.0e-262	100.00	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438639 P...	[more]
A0A6J1HJA3	1.3e-252	96.54	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465026 PE=...	[more]
A0A6J1KYS6	1.5e-211	81.09	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498785 PE=...	[more]
A0A6J1GIP4	5.6e-211	80.87	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454517 P...	[more]
A0A0A0L687	1.1e-206	78.52	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G073920 PE=3...	[more]

BLAST of CmoCh16G009420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932287.1	1.4e-261	100.00	glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata]	[more]
KAG6577464.1	5.5e-261	99.57	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro...	[more]
KAG7015534.1	2.1e-260	99.35	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argy...	[more]
XP_023551952.1	7.2e-253	97.38	glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo]	[more]
XP_022965047.1	2.7e-252	96.54	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima]	[more]

BLAST of CmoCh16G009420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55180.1	1.0e-188	72.10	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.2	9.2e-182	71.87	O-Glycosyl hydrolases family 17 protein	[more]
AT4G26830.1	4.6e-181	69.45	O-Glycosyl hydrolases family 17 protein	[more]
AT2G05790.1	2.3e-172	64.44	O-Glycosyl hydrolases family 17 protein	[more]
AT5G56590.1	5.9e-104	41.92	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 372..457 e-value: 5.1E-48 score: 175.5
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 372..443 e-value: 5.0E-23 score: 81.6
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 24..351 e-value: 6.5E-127 score: 424.9
None	No IPR available	GENE3D	1.20.58.1040		coord: 367..457 e-value: 3.7E-11 score: 45.3
None	No IPR available	PANTHER	PTHR32227:SF299	O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN	coord: 10..454
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 25..342 e-value: 4.9E-92 score: 308.9
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 257..270
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 10..454
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 25..342

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh16G009420.1	CmoCh16G009420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds