CmoCh16G007800 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G007800
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionATP-dependent 6-phosphofructokinase
LocationCmo_Chr16: 3943064 .. 3946795 (+)
RNA-Seq ExpressionCmoCh16G007800
SyntenyCmoCh16G007800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCATGGTCGTCAAGTCAAAACGGGAAAACCAGACGGCCACCACTTTTGTTCGAAGTTCGTATCGGACTGGACGTTCGTCTCCGTCCGTACTGTAGCTCTGGAGAAACAATTCTCATTGTCGCCAGTTCTTCAATCCTCTGTTCCTCATACTGGACGTTGTTCCTCCATTGTTCGAACCGATCGCCGTTTGATGGGTTCTATCTCGCACGCGATCGGTCCCAAACTCGGATTTCATCACCAGGTATCGCACTGTTCCAATGGACTGTTCGGTTTTAATGGTGATAAGTTTTTGAGTCTGAGTTCGAGGAAGCTTTTTGGAAGAATTTGTGCCTCTGTTGATGTTAAGGATGAACGCAAGAATACAGCGATTGATTTTAGTGATCCTGATTGGAAAATGAAGTACCAGGCGGACTTCGAAAAGCGCTTCAGTCTCCCTCACATTACCGACGTATTCGGAGATGCTCTTCCTATTCCTTCCACTTTTTGCCTCAAGATGAGGTGTTGTTCTCTTTTTTCCTTTAGTTTCTGGTAACATGATTCCTTTTGTTTGTTTGTTTGGTATGAATGTCGATATTTACTATGTGTGTTTGTATGGATTGGAATTGCAGGTCACCACCGGTTTCGGATGCCTTTGCTGGAGGTTATCCGTCAGATGAAGAGTGGCATGGATACGTAAATAAAAATGATCGTGTACTTCTCAAGGTATATTTTCTGTCACTGTTATGGTGAAAACATTGTGGAAGTTGATTGGTTGGTCATCATTTATTTCAACACCGTATTGAAGGTTTTTCCTTATTTCGCGAACTATTTCCATTTTCTTTTCACTTTAGTTTCTGCCATATTCTTCCTGATCATTCATTATGTTATGAATAGTAACGAAGCTTAAGCTATTGGATGATCATTTAAGATATATTCTAAACACTCCATGACATGTAGGCTCTGAATATTTGTTTGCTACATGTGATACTAGAACTTGTTCTTGAGGCCTGTTTTCACTTTCTAATCTGGGTAACTTGAAAATTTCTCGTCTATATTATAATGACATGCAGTTTTGTTATTGTTTTTGAGAAATTTAGTGAATTTACTCTTACTTTTGGTTTTATTGTGCACTGCCCAGGTCATAAATTACTCATCACGTAGTTCCGCCGGTGCTGAGTGCATTGACCCCAATTGTACATGGGTAGAACAATGGTAAAGTCTATTCAACTTTAGCCATTAACGATTGTAGGTCATGCCTGCATTGGTATAATTGAGCTGAAAGTTTTAATACTAACCTTTTTCCAGAACTGTTGAAGTGATTGTTAATTTTCTAGAACCTTAAAAGACTGCTTGAAGTTCAATTATGGGGTGAACTGAAACTATGGCTAAGAGGGGAAATCACTTGGCAGTGTGGACACTATGGGTTATTTAGCTTGTCAAGGGGATCATATATTGCTAAGACGCTATGGGTTATTTAGCTTGACAAGGGGATCATAGGGCTTCACAAATTTGGAAAAGAATTTCTTGCATATTTTCTTTTTCAAAGTAACTAATGCATTTTGATTCTTAATTACATCCATGCATAATATAACAATTCTAACTTATAGGGTTCATCGTGCTGGGCCTCGAGAGAAAATATATTTTAAACCAGAAGAAGTGAAAGCAGCAATTGTGACTTGTGGAGGTCTCTGTCCTGGCCTTAATGATGTTATCAGACAGGTTATTTTCATTATATCATTATTTTACTTTCGACTTCAGTTGCAAAAGTTATTTTATTTTATTTTTTTATTTCATGAACTTAAGCAGATTTCATTTTTAGATGTTATTGCGTCAATTATTTCTAGAAGGATATGCACTCTAGATTTTATATGTTAGCTTGTTTTGGAAGAGGTTTTCAGCATATTTTTTCTACGTTCTCAGAGAATATGCATTCTTATACAGATTGTAATCACACTTGAAATTTATGGTGTAAAGAAGATAGTTGGGATTCCATTTGGATATCGCGGATTCTCTGACAAAGAACTGACAGAAATGCCGGTAGGTTGCTCTTTGGCATGCATAGAATTATATTTTCTGTAGAACCACATGTCTTATGACAGTGCTGCTTCTCACACGACAATGTGGTTGAAAGAGTTGCCTTAGCTGAAGCTGTTTAATTGACTTTTTGGTGGCGTTCATTCCTAGTTGTCCAGAAAGGTGGTTCAGAACATTCATCTTTCTGGTGGAAGTTTGTTAGGAGTTTCGCGTGGAGGTCCTACCATTGACGAAATTGTTGACAGCTTGCAGGTCTGAAACAGACTCTCTTAGTTCGATAATGTGGAGTTTTTTTGGGAGTAGACCATTTGAAAATCATTGAGGCACATATTGTCCGTGTTTCAGGAAAGGGGAATTGATTTGCTTTTTGTTTTGGGTGGAAATGGCACACATGCGGGGGCTAATGTGATACACAACGAGGTAGGTTATAGCCTCTCTAACTTGACAATTTTGTATACTAGTTTCTCCTGATGTGCAGTGAGTACATTTGTGAATTTTGTTGTTGTACTAATATTATCTTCTTTTTCATGATCTATTGTTAAGTTTGATTTCTACGTTTTGCATTCTAAATTACGCTAGAAAAATTAGTGATAAATTGAGTTTACAACGCAAATTTGCAGTATTTTGGAATGCTCTCACTCTATATGTTTTTTCAATACTTCAAGCTCACCTCACAAGCGTGTCATATACTGGAATCTATCGTTCTTCGAGAACATGAATCCAATAGGCATTGTATGATATTGCAGAACCAATTCTAAAGCTACATGAATTTAATTAGAATCACTCTGATCTTTCTTCAGTCTTCACTCCACTTTAATAAAGTGTGGATTTTCATTTAGCGAGAGTATGATACTTTTTGTTCCTATGATTATTATTATGGTCTTTGATTGTAACGAGACATCTCTGCTTCTTAATAGAAAAAGCAACACCATTTCTGACTTTGTTGAATATTTTATTTTTTGTTTAGTGTCGAAAAAGACGATTAAAGGTGGCCGTGGTTGGCGTTCCAAAAACTATTGACAATGACATCTTATTGATGGATAAAACTTTTGGTTTTGATACTGCTGTTGAAGAAGCCCAACGAGCCATAAACTCTGCCTACATTGAGGTAAAGTGCTTTCTATTGCTGAATCAATACTGCCGTGTTCATGTGCAACTTTCCTTCCTCATTCTGGAAGTTGTCATAATTATGTTTAAAAATTGGTGTAGGCACATAGTGCTTATCATGGAGTTGGCATTGTGAAATTGATGGGTCGCAGCAGTGGTTTTATAGCAATGCAAGCAGCACTTGCTAGTGGGCAGATTGATGTATGCTTGATCCCTGAGGTATGAGTCAAAATATCAATGTAACATTTAGTTTCTATTTACTTGATGCCATACTGATATCACCAATGGTTCTTAGGTTCCTTTCCAACTACATGGGCCCCATGGTGTTCTACGCCATTTGAAATATCTCATAGAGACAAAGGGGTCTGCTGTAGTCTGTGTGGCTGAGGGAGCAGGGCAGGTACTCGGTATTGTTATTTTTATGAATCTTTTAATTTCTTTTTTGACTATGTTTATCATAAATGATTAGAGTGAAATACTGATGCTTTGCGTTAACTGCAGAATTTGGTACAAAAAACAAATGCCACCGATGCATCTGGGAACATTATACTAGGTGATATTGGCGTATATATTCAACAAGAGGTTTGTAAATTTACTTCCATGTAGA

mRNA sequence

ATGGAGCATGGTCGTCAAGTCAAAACGGGAAAACCAGACGGCCACCACTTTTGTTCGAAGTTCGTATCGGACTGGACGTTCGTCTCCGTCCGTACTGTAGCTCTGGAGAAACAATTCTCATTGTCGCCAGTTCTTCAATCCTCTGTTCCTCATACTGGACGTTGTTCCTCCATTGTTCGAACCGATCGCCGTTTGATGGGTTCTATCTCGCACGCGATCGGTCCCAAACTCGGATTTCATCACCAGGTATCGCACTGTTCCAATGGACTGTTCGGTTTTAATGGTGATAAGTTTTTGAGTCTGAGTTCGAGGAAGCTTTTTGGAAGAATTTGTGCCTCTGTTGATGTTAAGGATGAACGCAAGAATACAGCGATTGATTTTAGTGATCCTGATTGGAAAATGAAGTACCAGGCGGACTTCGAAAAGCGCTTCAGTCTCCCTCACATTACCGACGTATTCGGAGATGCTCTTCCTATTCCTTCCACTTTTTGCCTCAAGATGAGGTCACCACCGGTTTCGGATGCCTTTGCTGGAGGTTATCCGTCAGATGAAGAGTGGCATGGATACGTAAATAAAAATGATCGTGTACTTCTCAAGGTCATAAATTACTCATCACGTAGTTCCGCCGGTGCTGAGTGCATTGACCCCAATTGTACATGGGTAGAACAATGGGTTCATCGTGCTGGGCCTCGAGAGAAAATATATTTTAAACCAGAAGAAGTGAAAGCAGCAATTGTGACTTGTGGAGGTCTCTGTCCTGGCCTTAATGATGTTATCAGACAGATTGTAATCACACTTGAAATTTATGGTGTAAAGAAGATAGTTGGGATTCCATTTGGATATCGCGGATTCTCTGACAAAGAACTGACAGAAATGCCGTTGTCCAGAAAGGTGGTTCAGAACATTCATCTTTCTGGTGGAAGTTTGTTAGGAGTTTCGCGTGGAGGTCCTACCATTGACGAAATTGTTGACAGCTTGCAGGAAAGGGGAATTGATTTGCTTTTTGTTTTGGGTGGAAATGGCACACATGCGGGGGCTAATGTGATACACAACGAGTGTCGAAAAAGACGATTAAAGGTGGCCGTGGTTGGCGTTCCAAAAACTATTGACAATGACATCTTATTGATGGATAAAACTTTTGGTTTTGATACTGCTGTTGAAGAAGCCCAACGAGCCATAAACTCTGCCTACATTGAGGCACATAGTGCTTATCATGGAGTTGGCATTGTGAAATTGATGGGTCGCAGCAGTGGTTTTATAGCAATGCAAGCAGCACTTGCTAGTGGGCAGATTGATGTATGCTTGATCCCTGAGGTTCCTTTCCAACTACATGGGCCCCATGGTGTTCTACGCCATTTGAAATATCTCATAGAGACAAAGGGGTCTGCTGTAGTCTGTGTGGCTGAGGGAGCAGGGCAGAGTGAAATACTGATGCTTTGCGTTAACTGCAGAATTTGA

Coding sequence (CDS)

ATGGAGCATGGTCGTCAAGTCAAAACGGGAAAACCAGACGGCCACCACTTTTGTTCGAAGTTCGTATCGGACTGGACGTTCGTCTCCGTCCGTACTGTAGCTCTGGAGAAACAATTCTCATTGTCGCCAGTTCTTCAATCCTCTGTTCCTCATACTGGACGTTGTTCCTCCATTGTTCGAACCGATCGCCGTTTGATGGGTTCTATCTCGCACGCGATCGGTCCCAAACTCGGATTTCATCACCAGGTATCGCACTGTTCCAATGGACTGTTCGGTTTTAATGGTGATAAGTTTTTGAGTCTGAGTTCGAGGAAGCTTTTTGGAAGAATTTGTGCCTCTGTTGATGTTAAGGATGAACGCAAGAATACAGCGATTGATTTTAGTGATCCTGATTGGAAAATGAAGTACCAGGCGGACTTCGAAAAGCGCTTCAGTCTCCCTCACATTACCGACGTATTCGGAGATGCTCTTCCTATTCCTTCCACTTTTTGCCTCAAGATGAGGTCACCACCGGTTTCGGATGCCTTTGCTGGAGGTTATCCGTCAGATGAAGAGTGGCATGGATACGTAAATAAAAATGATCGTGTACTTCTCAAGGTCATAAATTACTCATCACGTAGTTCCGCCGGTGCTGAGTGCATTGACCCCAATTGTACATGGGTAGAACAATGGGTTCATCGTGCTGGGCCTCGAGAGAAAATATATTTTAAACCAGAAGAAGTGAAAGCAGCAATTGTGACTTGTGGAGGTCTCTGTCCTGGCCTTAATGATGTTATCAGACAGATTGTAATCACACTTGAAATTTATGGTGTAAAGAAGATAGTTGGGATTCCATTTGGATATCGCGGATTCTCTGACAAAGAACTGACAGAAATGCCGTTGTCCAGAAAGGTGGTTCAGAACATTCATCTTTCTGGTGGAAGTTTGTTAGGAGTTTCGCGTGGAGGTCCTACCATTGACGAAATTGTTGACAGCTTGCAGGAAAGGGGAATTGATTTGCTTTTTGTTTTGGGTGGAAATGGCACACATGCGGGGGCTAATGTGATACACAACGAGTGTCGAAAAAGACGATTAAAGGTGGCCGTGGTTGGCGTTCCAAAAACTATTGACAATGACATCTTATTGATGGATAAAACTTTTGGTTTTGATACTGCTGTTGAAGAAGCCCAACGAGCCATAAACTCTGCCTACATTGAGGCACATAGTGCTTATCATGGAGTTGGCATTGTGAAATTGATGGGTCGCAGCAGTGGTTTTATAGCAATGCAAGCAGCACTTGCTAGTGGGCAGATTGATGTATGCTTGATCCCTGAGGTTCCTTTCCAACTACATGGGCCCCATGGTGTTCTACGCCATTTGAAATATCTCATAGAGACAAAGGGGTCTGCTGTAGTCTGTGTGGCTGAGGGAGCAGGGCAGAGTGAAATACTGATGCTTTGCGTTAACTGCAGAATTTGA

Protein sequence

MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVRTDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDERKNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGYPSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQSEILMLCVNCRI
Homology
BLAST of CmoCh16G007800 vs. ExPASy Swiss-Prot
Match: Q8VYN6 (ATP-dependent 6-phosphofructokinase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK5 PE=1 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.5e-182
Identity = 296/352 (84.09%), Postives = 332/352 (94.32%), Query Frame = 0

Query: 123 TAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGYPS 182
           ++ID SDPDWK+KY+ DFE+RFS+PHITDV  DA  I STFCLKMRSP  ++ F GGYPS
Sbjct: 64  SSIDLSDPDWKLKYEKDFEQRFSIPHITDVLPDAEAIRSTFCLKMRSP--TEDFVGGYPS 123

Query: 183 DEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVK 242
           DEEWHGY+N NDRVLLKVI+YSS +SAGAEC+D +C+WVEQW+HRAGPREKIYF+PEEVK
Sbjct: 124 DEEWHGYINNNDRVLLKVISYSSPTSAGAECLDHDCSWVEQWIHRAGPREKIYFRPEEVK 183

Query: 243 AAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNI 302
           AAI+TCGGLCPGLNDVIR IVITLEIYGVK IVGIPFGYRGFSDK+LTEMPLSRKVVQNI
Sbjct: 184 AAIITCGGLCPGLNDVIRHIVITLEIYGVKNIVGIPFGYRGFSDKDLTEMPLSRKVVQNI 243

Query: 303 HLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAV 362
           HLSGGSLLGVSRGGP++ EIVDS++ERGI++LFVLGGNGTHAGAN IHNECRKR++KVAV
Sbjct: 244 HLSGGSLLGVSRGGPSVSEIVDSMEERGINMLFVLGGNGTHAGANAIHNECRKRKIKVAV 303

Query: 363 VGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAM 422
           VGVPKTIDNDIL MDKTFGFDTAVEEAQRAINSAYIEAHSAYHG+G+VKLMGR+SGFIAM
Sbjct: 304 VGVPKTIDNDILHMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGIGVVKLMGRNSGFIAM 363

Query: 423 QAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           QA+LASGQ+D+CLIPEVPF LHGP+GVL+HLKYLIETKGSAV+CVAEGAGQ+
Sbjct: 364 QASLASGQVDICLIPEVPFNLHGPNGVLKHLKYLIETKGSAVICVAEGAGQN 413

BLAST of CmoCh16G007800 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 5.5e-81
Identity = 147/248 (59.27%), Postives = 193/248 (77.82%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+K+YF+ +EV A IVTCGGLCPGLN VIR++V +L  +YGVK+I+GI  GYRGF 
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            K    +PL+ KVV +IH  GG+++G SRGG   ++IVDS+Q+RGI+ ++++GG+GT  G
Sbjct: 138 AK--NTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRG 197

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           A+VI  E R+RRLKVAVVG+PKTIDNDI ++DK+FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 198 ASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNEN 257

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+G VKLMGR SG+IAM A LAS  +D CLIPE PF L G  G+   ++  ++  G  V+
Sbjct: 258 GIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVI 317

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 318 VLAEGAGQ 323

BLAST of CmoCh16G007800 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.1e-80
Identity = 147/248 (59.27%), Postives = 191/248 (77.02%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+K+YF+ +EV A IVTCGGLCPGLN VIR+IV +L  +YGVK+I+GI  GYRGF 
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            K    + L  KVV +IH  GG++LG SRGG    +IVDS+Q+RGI+ ++++GG+GT  G
Sbjct: 138 AK--NTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRG 197

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           A+VI  E R+R LKVAV+G+PKTIDNDI ++DK+FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 198 ASVIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIEN 257

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+G+VKLMGR SGFIAM A LAS  +D CLIPE PF L G  G+  +++  ++  G  V+
Sbjct: 258 GIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVL 317

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 318 VIAEGAGQ 323

BLAST of CmoCh16G007800 vs. ExPASy Swiss-Prot
Match: Q9M076 (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.6e-78
Identity = 142/248 (57.26%), Postives = 185/248 (74.60%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+K+YFKP +V+A IVTCGGLCPGLN VIR+IV  L  +YGV +++G+  G+RGF 
Sbjct: 79  RAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCGFRGFY 138

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            K    + L+ K V +IH  GG++LG SRGG    +IVD++Q+R I+ ++++GG+GT  G
Sbjct: 139 SK--NTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGTQKG 198

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           AN I+ E R+R LKVAV G+PKTIDNDI ++DK+FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 199 ANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATSVEN 258

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+GIVKLMGR SGFIAM A LAS  +D CLIPE PF L G  G+   +   +   G  V+
Sbjct: 259 GIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGHMVI 318

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 319 VIAEGAGQ 324

BLAST of CmoCh16G007800 vs. ExPASy Swiss-Prot
Match: Q9M0F9 (ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.2e-77
Identity = 142/248 (57.26%), Postives = 186/248 (75.00%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+++YF+ ++V A IVTCGGLCPGLN VIR+IV  L  +YGVK+I+GI  GYRGF 
Sbjct: 79  RAGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVKRILGIDGGYRGFY 138

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            +    + L  K V +IH SGG++LG SRGG    +IVDS+Q+RGI+ ++++GG+G+  G
Sbjct: 139 ARNTIHLDL--KTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKG 198

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           A  I  E RKR+LKVAV G+PKTIDNDI ++D++FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 199 AAAIFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAINAAHVEATSFEN 258

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+G+VKLMGR SGFIAM A LAS  +D CLIPE PF L G  G+   +   ++  G  V+
Sbjct: 259 GIGLVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFIDKRLKESGHMVI 318

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 319 VIAEGAGQ 324

BLAST of CmoCh16G007800 vs. ExPASy TrEMBL
Match: A0A6J1ERV2 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111435953 PE=3 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 7.5e-283
Identity = 473/474 (99.79%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60
           MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR
Sbjct: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60

Query: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120
           TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER
Sbjct: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120

Query: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180
           KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY
Sbjct: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180

Query: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240
           PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE
Sbjct: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240

Query: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300
           VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ
Sbjct: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300

Query: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360
           NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV
Sbjct: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360

Query: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420
           AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI
Sbjct: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420

Query: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ+
Sbjct: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQN 474

BLAST of CmoCh16G007800 vs. ExPASy TrEMBL
Match: A0A6J1ENH6 (ATP-dependent 6-phosphofructokinase 5, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435953 PE=4 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 9.8e-283
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60
           MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR
Sbjct: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60

Query: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120
           TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER
Sbjct: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120

Query: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180
           KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY
Sbjct: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180

Query: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240
           PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE
Sbjct: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240

Query: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300
           VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ
Sbjct: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300

Query: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360
           NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV
Sbjct: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360

Query: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420
           AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI
Sbjct: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420

Query: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ 474
           AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ
Sbjct: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ 473

BLAST of CmoCh16G007800 vs. ExPASy TrEMBL
Match: A0A6J1J8W9 (ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111482815 PE=3 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 8.6e-279
Identity = 468/479 (97.70%), Postives = 471/479 (98.33%), Query Frame = 0

Query: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQF-----SLSPVLQSSVPHTGRC 60
           MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQF      LSP+LQSSVPHTGRC
Sbjct: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFYIAYLLLSPILQSSVPHTGRC 60

Query: 61  SSIVRTDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVD 120
           SSIVRTDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVD
Sbjct: 61  SSIVRTDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVD 120

Query: 121 VKDERKNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDA 180
           V+DERKNT IDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDA
Sbjct: 121 VRDERKNTTIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDA 180

Query: 181 FAGGYPSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIY 240
           FAGGYPSDEEWHGYVN NDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIY
Sbjct: 181 FAGGYPSDEEWHGYVNNNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIY 240

Query: 241 FKPEEVKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLS 300
           FKPEEVKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLS
Sbjct: 241 FKPEEVKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLS 300

Query: 301 RKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRK 360
           RKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRK
Sbjct: 301 RKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRK 360

Query: 361 RRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGR 420
           RRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGR
Sbjct: 361 RRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGR 420

Query: 421 SSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           SSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ+
Sbjct: 421 SSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQN 479

BLAST of CmoCh16G007800 vs. ExPASy TrEMBL
Match: A0A6J1FU90 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111447341 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.7e-223
Identity = 378/409 (92.42%), Postives = 390/409 (95.35%), Query Frame = 0

Query: 66  MGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDERKNTAI 125
           MGSISH+IGPKLGFHHQVSHCS GLFG +  KFLSLSSRK FGRI AS+DVKD RK  AI
Sbjct: 1   MGSISHSIGPKLGFHHQVSHCSPGLFGLSRGKFLSLSSRKHFGRIHASIDVKDGRKIEAI 60

Query: 126 DFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGYPSDEE 185
           DFSDPDWK+KYQADFEKRF+LPHITDVFGDA+PIPSTFCLKMRS PVSD F G YPSDEE
Sbjct: 61  DFSDPDWKLKYQADFEKRFNLPHITDVFGDAVPIPSTFCLKMRS-PVSDVFPGVYPSDEE 120

Query: 186 WHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVKAAI 245
           WHGY+N NDRVLLKVINYSS +SAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVKAAI
Sbjct: 121 WHGYINNNDRVLLKVINYSSPTSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVKAAI 180

Query: 246 VTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIHLS 305
           VTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIHLS
Sbjct: 181 VTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIHLS 240

Query: 306 GGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAVVGV 365
           GGSLLGVSRGGP I EIVDSLQERGID+LFVLGGNGTHAGAN IHNECRKRRLKVAVVGV
Sbjct: 241 GGSLLGVSRGGPGIGEIVDSLQERGIDMLFVLGGNGTHAGANAIHNECRKRRLKVAVVGV 300

Query: 366 PKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAMQAA 425
           PKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVG+VKLMGRSSGFIAM AA
Sbjct: 301 PKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGVVKLMGRSSGFIAMHAA 360

Query: 426 LASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           LASGQIDVCL+PEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ+
Sbjct: 361 LASGQIDVCLVPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQN 408

BLAST of CmoCh16G007800 vs. ExPASy TrEMBL
Match: A0A6J1C8B3 (ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC111008358 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.7e-222
Identity = 376/411 (91.48%), Postives = 391/411 (95.13%), Query Frame = 0

Query: 66  MGSISHAIGPKLGFHHQVSHCSNGLFGF--NGDKFLSLSSRKLFGRICASVDVKDERKNT 125
           MGSISHAIGPK+GFHHQV HCS+G FGF  N D+FL LSSRK FGRI AS+DVKDE KN 
Sbjct: 1   MGSISHAIGPKVGFHHQVPHCSHGPFGFNLNSDQFLRLSSRKHFGRIRASLDVKDEHKNP 60

Query: 126 AIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGYPSD 185
           AIDFSDPDWK+KYQADFEKRF+LPHITDVF DA+PIPSTFCLKMR+ PVSD FAGGYPSD
Sbjct: 61  AIDFSDPDWKLKYQADFEKRFNLPHITDVFEDAIPIPSTFCLKMRT-PVSDVFAGGYPSD 120

Query: 186 EEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVKA 245
           EEWHGY+N NDRVLLKVINYSS  SAGAECIDPNCTWVEQWVHRAGPREKIYF+PEEVKA
Sbjct: 121 EEWHGYINNNDRVLLKVINYSSPRSAGAECIDPNCTWVEQWVHRAGPREKIYFQPEEVKA 180

Query: 246 AIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIH 305
           AIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIH
Sbjct: 181 AIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIH 240

Query: 306 LSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAVV 365
           LSGGSLLGVSRGGP I EIVDSLQERGID+LFVLGGNGTHAGAN IHNECRKRRLKVAVV
Sbjct: 241 LSGGSLLGVSRGGPGISEIVDSLQERGIDMLFVLGGNGTHAGANAIHNECRKRRLKVAVV 300

Query: 366 GVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAMQ 425
           GVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVG+VKLMGRSSGFIAM 
Sbjct: 301 GVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGVVKLMGRSSGFIAMH 360

Query: 426 AALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           A+LASGQIDVCLIPEVPFQLHGPHGVLRH+KYLIETKGSAVVCVAEGAGQ+
Sbjct: 361 ASLASGQIDVCLIPEVPFQLHGPHGVLRHVKYLIETKGSAVVCVAEGAGQN 410

BLAST of CmoCh16G007800 vs. NCBI nr
Match: XP_022929353.1 (ATP-dependent 6-phosphofructokinase 5, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6577313.1 ATP-dependent 6-phosphofructokinase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 982.2 bits (2538), Expect = 1.6e-282
Identity = 473/474 (99.79%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60
           MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR
Sbjct: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60

Query: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120
           TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER
Sbjct: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120

Query: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180
           KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY
Sbjct: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180

Query: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240
           PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE
Sbjct: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240

Query: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300
           VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ
Sbjct: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300

Query: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360
           NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV
Sbjct: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360

Query: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420
           AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI
Sbjct: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420

Query: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ+
Sbjct: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQN 474

BLAST of CmoCh16G007800 vs. NCBI nr
Match: XP_022929354.1 (ATP-dependent 6-phosphofructokinase 5, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 981.9 bits (2537), Expect = 2.0e-282
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60
           MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR
Sbjct: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60

Query: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120
           TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER
Sbjct: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120

Query: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180
           KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY
Sbjct: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180

Query: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240
           PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE
Sbjct: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240

Query: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300
           VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ
Sbjct: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300

Query: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360
           NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV
Sbjct: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360

Query: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420
           AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI
Sbjct: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420

Query: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ 474
           AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ
Sbjct: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ 473

BLAST of CmoCh16G007800 vs. NCBI nr
Match: KAG7015404.1 (ATP-dependent 6-phosphofructokinase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 979.9 bits (2532), Expect = 7.7e-282
Identity = 472/474 (99.58%), Postives = 473/474 (99.79%), Query Frame = 0

Query: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60
           MEHGRQVKTGKPDGHHFCSKFVSDW FVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR
Sbjct: 1   MEHGRQVKTGKPDGHHFCSKFVSDWKFVSVRTVALEKQFSLSPVLQSSVPHTGRCSSIVR 60

Query: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120
           TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER
Sbjct: 61  TDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDER 120

Query: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180
           KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY
Sbjct: 121 KNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGY 180

Query: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240
           PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE
Sbjct: 181 PSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEE 240

Query: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300
           VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ
Sbjct: 241 VKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQ 300

Query: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360
           NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV
Sbjct: 301 NIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKV 360

Query: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420
           AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI
Sbjct: 361 AVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFI 420

Query: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ+
Sbjct: 421 AMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQN 474

BLAST of CmoCh16G007800 vs. NCBI nr
Match: XP_022984555.1 (ATP-dependent 6-phosphofructokinase 5, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 968.8 bits (2503), Expect = 1.8e-278
Identity = 468/479 (97.70%), Postives = 471/479 (98.33%), Query Frame = 0

Query: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQF-----SLSPVLQSSVPHTGRC 60
           MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQF      LSP+LQSSVPHTGRC
Sbjct: 1   MEHGRQVKTGKPDGHHFCSKFVSDWTFVSVRTVALEKQFYIAYLLLSPILQSSVPHTGRC 60

Query: 61  SSIVRTDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVD 120
           SSIVRTDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVD
Sbjct: 61  SSIVRTDRRLMGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVD 120

Query: 121 VKDERKNTAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDA 180
           V+DERKNT IDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDA
Sbjct: 121 VRDERKNTTIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDA 180

Query: 181 FAGGYPSDEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIY 240
           FAGGYPSDEEWHGYVN NDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIY
Sbjct: 181 FAGGYPSDEEWHGYVNNNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIY 240

Query: 241 FKPEEVKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLS 300
           FKPEEVKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLS
Sbjct: 241 FKPEEVKAAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLS 300

Query: 301 RKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRK 360
           RKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRK
Sbjct: 301 RKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRK 360

Query: 361 RRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGR 420
           RRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGR
Sbjct: 361 RRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGR 420

Query: 421 SSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           SSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ+
Sbjct: 421 SSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQN 479

BLAST of CmoCh16G007800 vs. NCBI nr
Match: XP_023551999.1 (ATP-dependent 6-phosphofructokinase 5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840.1 bits (2169), Expect = 9.6e-240
Identity = 404/409 (98.78%), Postives = 406/409 (99.27%), Query Frame = 0

Query: 66  MGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDERKNTAI 125
           MGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDERKNTAI
Sbjct: 1   MGSISHAIGPKLGFHHQVSHCSNGLFGFNGDKFLSLSSRKLFGRICASVDVKDERKNTAI 60

Query: 126 DFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGYPSDEE 185
           DFSDPDWKMKYQADFEKRFSLPHITDVFGDA+PIPSTFCLKMRSPPVSDAFAGGYPSDEE
Sbjct: 61  DFSDPDWKMKYQADFEKRFSLPHITDVFGDAVPIPSTFCLKMRSPPVSDAFAGGYPSDEE 120

Query: 186 WHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVKAAI 245
           WHGYVN NDRVLLKVINYSSR SAGAECIDPNCTWVEQWVHRAGPREKIYFKPE VKAAI
Sbjct: 121 WHGYVNNNDRVLLKVINYSSRRSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEAVKAAI 180

Query: 246 VTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIHLS 305
           VTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIHLS
Sbjct: 181 VTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNIHLS 240

Query: 306 GGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAVVGV 365
           GGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAVVGV
Sbjct: 241 GGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAVVGV 300

Query: 366 PKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAMQAA 425
           PKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAMQAA
Sbjct: 301 PKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAMQAA 360

Query: 426 LASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           LASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQ+
Sbjct: 361 LASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQN 409

BLAST of CmoCh16G007800 vs. TAIR 10
Match: AT2G22480.1 (phosphofructokinase 5 )

HSP 1 Score: 640.6 bits (1651), Expect = 1.0e-183
Identity = 296/352 (84.09%), Postives = 332/352 (94.32%), Query Frame = 0

Query: 123 TAIDFSDPDWKMKYQADFEKRFSLPHITDVFGDALPIPSTFCLKMRSPPVSDAFAGGYPS 182
           ++ID SDPDWK+KY+ DFE+RFS+PHITDV  DA  I STFCLKMRSP  ++ F GGYPS
Sbjct: 64  SSIDLSDPDWKLKYEKDFEQRFSIPHITDVLPDAEAIRSTFCLKMRSP--TEDFVGGYPS 123

Query: 183 DEEWHGYVNKNDRVLLKVINYSSRSSAGAECIDPNCTWVEQWVHRAGPREKIYFKPEEVK 242
           DEEWHGY+N NDRVLLKVI+YSS +SAGAEC+D +C+WVEQW+HRAGPREKIYF+PEEVK
Sbjct: 124 DEEWHGYINNNDRVLLKVISYSSPTSAGAECLDHDCSWVEQWIHRAGPREKIYFRPEEVK 183

Query: 243 AAIVTCGGLCPGLNDVIRQIVITLEIYGVKKIVGIPFGYRGFSDKELTEMPLSRKVVQNI 302
           AAI+TCGGLCPGLNDVIR IVITLEIYGVK IVGIPFGYRGFSDK+LTEMPLSRKVVQNI
Sbjct: 184 AAIITCGGLCPGLNDVIRHIVITLEIYGVKNIVGIPFGYRGFSDKDLTEMPLSRKVVQNI 243

Query: 303 HLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAGANVIHNECRKRRLKVAV 362
           HLSGGSLLGVSRGGP++ EIVDS++ERGI++LFVLGGNGTHAGAN IHNECRKR++KVAV
Sbjct: 244 HLSGGSLLGVSRGGPSVSEIVDSMEERGINMLFVLGGNGTHAGANAIHNECRKRKIKVAV 303

Query: 363 VGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGVGIVKLMGRSSGFIAM 422
           VGVPKTIDNDIL MDKTFGFDTAVEEAQRAINSAYIEAHSAYHG+G+VKLMGR+SGFIAM
Sbjct: 304 VGVPKTIDNDILHMDKTFGFDTAVEEAQRAINSAYIEAHSAYHGIGVVKLMGRNSGFIAM 363

Query: 423 QAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVVCVAEGAGQS 475
           QA+LASGQ+D+CLIPEVPF LHGP+GVL+HLKYLIETKGSAV+CVAEGAGQ+
Sbjct: 364 QASLASGQVDICLIPEVPFNLHGPNGVLKHLKYLIETKGSAVICVAEGAGQN 413

BLAST of CmoCh16G007800 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 303.1 bits (775), Expect = 3.9e-82
Identity = 147/248 (59.27%), Postives = 193/248 (77.82%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+K+YF+ +EV A IVTCGGLCPGLN VIR++V +L  +YGVK+I+GI  GYRGF 
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            K    +PL+ KVV +IH  GG+++G SRGG   ++IVDS+Q+RGI+ ++++GG+GT  G
Sbjct: 138 AK--NTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRG 197

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           A+VI  E R+RRLKVAVVG+PKTIDNDI ++DK+FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 198 ASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNEN 257

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+G VKLMGR SG+IAM A LAS  +D CLIPE PF L G  G+   ++  ++  G  V+
Sbjct: 258 GIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVI 317

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 318 VLAEGAGQ 323

BLAST of CmoCh16G007800 vs. TAIR 10
Match: AT4G26270.1 (phosphofructokinase 3 )

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-81
Identity = 147/248 (59.27%), Postives = 191/248 (77.02%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+K+YF+ +EV A IVTCGGLCPGLN VIR+IV +L  +YGVK+I+GI  GYRGF 
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            K    + L  KVV +IH  GG++LG SRGG    +IVDS+Q+RGI+ ++++GG+GT  G
Sbjct: 138 AK--NTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRG 197

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           A+VI  E R+R LKVAV+G+PKTIDNDI ++DK+FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 198 ASVIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIEN 257

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+G+VKLMGR SGFIAM A LAS  +D CLIPE PF L G  G+  +++  ++  G  V+
Sbjct: 258 GIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVL 317

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 318 VIAEGAGQ 323

BLAST of CmoCh16G007800 vs. TAIR 10
Match: AT4G32840.1 (phosphofructokinase 6 )

HSP 1 Score: 294.3 bits (752), Expect = 1.8e-79
Identity = 142/248 (57.26%), Postives = 185/248 (74.60%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+K+YFKP +V+A IVTCGGLCPGLN VIR+IV  L  +YGV +++G+  G+RGF 
Sbjct: 79  RAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCGFRGFY 138

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            K    + L+ K V +IH  GG++LG SRGG    +IVD++Q+R I+ ++++GG+GT  G
Sbjct: 139 SK--NTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGTQKG 198

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           AN I+ E R+R LKVAV G+PKTIDNDI ++DK+FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 199 ANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATSVEN 258

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+GIVKLMGR SGFIAM A LAS  +D CLIPE PF L G  G+   +   +   G  V+
Sbjct: 259 GIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGHMVI 318

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 319 VIAEGAGQ 324

BLAST of CmoCh16G007800 vs. TAIR 10
Match: AT4G29220.1 (phosphofructokinase 1 )

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-78
Identity = 142/248 (57.26%), Postives = 186/248 (75.00%), Query Frame = 0

Query: 227 RAGPREKIYFKPEEVKAAIVTCGGLCPGLNDVIRQIVITLE-IYGVKKIVGIPFGYRGFS 286
           RAGPR+++YF+ ++V A IVTCGGLCPGLN VIR+IV  L  +YGVK+I+GI  GYRGF 
Sbjct: 79  RAGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVKRILGIDGGYRGFY 138

Query: 287 DKELTEMPLSRKVVQNIHLSGGSLLGVSRGGPTIDEIVDSLQERGIDLLFVLGGNGTHAG 346
            +    + L  K V +IH SGG++LG SRGG    +IVDS+Q+RGI+ ++++GG+G+  G
Sbjct: 139 ARNTIHLDL--KTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKG 198

Query: 347 ANVIHNECRKRRLKVAVVGVPKTIDNDILLMDKTFGFDTAVEEAQRAINSAYIEAHSAYH 406
           A  I  E RKR+LKVAV G+PKTIDNDI ++D++FGFDTAVEEAQRAIN+A++EA S  +
Sbjct: 199 AAAIFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAINAAHVEATSFEN 258

Query: 407 GVGIVKLMGRSSGFIAMQAALASGQIDVCLIPEVPFQLHGPHGVLRHLKYLIETKGSAVV 466
           G+G+VKLMGR SGFIAM A LAS  +D CLIPE PF L G  G+   +   ++  G  V+
Sbjct: 259 GIGLVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFIDKRLKESGHMVI 318

Query: 467 CVAEGAGQ 474
            +AEGAGQ
Sbjct: 319 VIAEGAGQ 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYN61.5e-18284.09ATP-dependent 6-phosphofructokinase 5, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9C5J75.5e-8159.27ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE... [more]
Q94AA42.1e-8059.27ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE... [more]
Q9M0762.6e-7857.26ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE... [more]
Q9M0F92.2e-7757.26ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1ERV27.5e-28399.79ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11143595... [more]
A0A6J1ENH69.8e-283100.00ATP-dependent 6-phosphofructokinase 5, chloroplastic-like isoform X2 OS=Cucurbit... [more]
A0A6J1J8W98.6e-27997.70ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111482815 ... [more]
A0A6J1FU906.7e-22392.42ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11144734... [more]
A0A6J1C8B35.7e-22291.48ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC1110083... [more]
Match NameE-valueIdentityDescription
XP_022929353.11.6e-28299.79ATP-dependent 6-phosphofructokinase 5, chloroplastic-like isoform X1 [Cucurbita ... [more]
XP_022929354.12.0e-282100.00ATP-dependent 6-phosphofructokinase 5, chloroplastic-like isoform X2 [Cucurbita ... [more]
KAG7015404.17.7e-28299.58ATP-dependent 6-phosphofructokinase 5, chloroplastic, partial [Cucurbita argyros... [more]
XP_022984555.11.8e-27897.70ATP-dependent 6-phosphofructokinase 5, chloroplastic-like [Cucurbita maxima][more]
XP_023551999.19.6e-24098.78ATP-dependent 6-phosphofructokinase 5, chloroplastic-like [Cucurbita pepo subsp.... [more]
Match NameE-valueIdentityDescription
AT2G22480.11.0e-18384.09phosphofructokinase 5 [more]
AT5G56630.13.9e-8259.27phosphofructokinase 7 [more]
AT4G26270.11.5e-8159.27phosphofructokinase 3 [more]
AT4G32840.11.8e-7957.26phosphofructokinase 6 [more]
AT4G29220.11.5e-7857.26phosphofructokinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 420..437
score: 26.35
coord: 459..471
score: 39.45
coord: 402..418
score: 29.86
coord: 363..380
score: 50.28
coord: 245..264
score: 42.31
coord: 330..346
score: 51.73
coord: 381..399
score: 28.07
coord: 271..284
score: 30.59
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 243..473
e-value: 4.5E-58
score: 196.8
NoneNo IPR availableGENE3D3.40.50.450coord: 237..428
e-value: 1.2E-58
score: 199.5
NoneNo IPR availablePANTHERPTHR45770FAMILY NOT NAMEDcoord: 108..474
NoneNo IPR availablePANTHERPTHR45770:SF23ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 5, CHLOROPLASTICcoord: 108..474
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 218..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G007800.1CmoCh16G007800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding