CmoCh16G007500 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G007500
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionEthylene-insensitive protein 3
LocationCmo_Chr16: 3765531 .. 3766895 (+)
RNA-Seq ExpressionCmoCh16G007500
SyntenyCmoCh16G007500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGTGGAATTCCATGGAGAAATCCGAAGCTCCGGCGATGGAGACGAGCAAGACAAGGAAGAGGAACTTAGCTACGATGATCTCAAGAAACGGATGTGGAAAGATAGACAGAGGATGAAGCAGCTGAAGGAGAAGCGGGATACTGAAGAACCTGAATCCACCGCTAGAGAAGAAGCCTCTCGCCGGAAAAAAATGGCCAGAGCTCAAGATTCGATCCTTAAGTACATGGATAAGATAATGGACGCTTGCAAGGCAAAAGGGTTTGTTTATGGGATCGTTCCGGAGAAGGGGAAACCAGTCACCGGCTCCTCGGAGAGCCTCCGGGAGTGGTGGCGGGACGACGTCCGGTTCGAACAAGATGCTCCCTTAGCCATAGCTCAATTCCTCCCGAAAGTTCTCGAAGAAACAGGGCTCGATTCGGAGTCGTTTTTGCACATGCTTCATGATTTACAGGACACAACATTGGGGTCGATTCTCTCTGCTTTGATTCAACACTGTATTCCGCCACAGAGGAAATTCCCACTTGAGAAGGGTTTAGCGCCGCCGTGGTGGCCGACGGGGAATGAGCTTTGGTGGGGTGAACAGGGTGCTGCTGGTGGACATGGCGTTCCGCCGTATAAAAAGCCTCACGATCTCAAAAAGGCATGGAAAATTAGTGTCTTGGCAGCAGTGATCAAACATATGTCCCCTGATTTGGACAATATGAGGAAATTGGTTCGACAATCGAAGAATTTACAAGCGAAAATGACGGCAAAGGAGACGCTTACGTGGGCTAAAGTCGTGAATCAAGAAGAATCCCTCTTGCAGCTAACAAAAAAAAGCCTCAAAATCACATCCGTCGAAGACGGACATGACGAAAACAGTGAAGAACAGGACGCCGGTGGGATTATTACGAAACGTAAGTATATGTTCGAACAAGTAGCTGCAGCAGCAGCCGATTCGGCGCTGTACGCTTGTCAGAACCAACGTTGCCCACAGAGCGAGGCGCCGATGGGATTCGCCGACAAGAACGCAAGAACAGAGCATGAGACGCAGTGCATTTACAGAGAAGCAGAGGATGAGAACATCGACACGCATTTGAAGAGCGTGGTGGAGTGGATGAACTGGGAGCTGTCGAGGGCGGAGCCAAGCAGCGAGGAGGGGAGGAGCGAAGACGCCGTAGATGGGTCCGGCAGCAGCACGGTGGAGAATTACGGGAGCAGGTACTGGAAGGGCGGCGTGGGAGAGGCGGCGGTGGATGAGGCGTTTGAGATGCATAGAGCGAGTATGGATTTGAATGCGAGTCCGGCGGAGGATTTGGGTGGTGGGCAACAAGATGCGACTTCCGTTTGGGATTTGATATATGAATGGGCTGAAGACTAA

mRNA sequence

ATGATGGTGGAATTCCATGGAGAAATCCGAAGCTCCGGCGATGGAGACGAGCAAGACAAGGAAGAGGAACTTAGCTACGATGATCTCAAGAAACGGATGTGGAAAGATAGACAGAGGATGAAGCAGCTGAAGGAGAAGCGGGATACTGAAGAACCTGAATCCACCGCTAGAGAAGAAGCCTCTCGCCGGAAAAAAATGGCCAGAGCTCAAGATTCGATCCTTAAGTACATGGATAAGATAATGGACGCTTGCAAGGCAAAAGGGTTTGTTTATGGGATCGTTCCGGAGAAGGGGAAACCAGTCACCGGCTCCTCGGAGAGCCTCCGGGAGTGGTGGCGGGACGACGTCCGGTTCGAACAAGATGCTCCCTTAGCCATAGCTCAATTCCTCCCGAAAGTTCTCGAAGAAACAGGGCTCGATTCGGAGTCGTTTTTGCACATGCTTCATGATTTACAGGACACAACATTGGGGTCGATTCTCTCTGCTTTGATTCAACACTGTATTCCGCCACAGAGGAAATTCCCACTTGAGAAGGGTTTAGCGCCGCCGTGGTGGCCGACGGGGAATGAGCTTTGGTGGGGTGAACAGGGTGCTGCTGGTGGACATGGCGTTCCGCCGTATAAAAAGCCTCACGATCTCAAAAAGGCATGGAAAATTAGTGTCTTGGCAGCAGTGATCAAACATATGTCCCCTGATTTGGACAATATGAGGAAATTGGTTCGACAATCGAAGAATTTACAAGCGAAAATGACGGCAAAGGAGACGCTTACGTGGGCTAAAGTCGTGAATCAAGAAGAATCCCTCTTGCAGCTAACAAAAAAAAGCCTCAAAATCACATCCGTCGAAGACGGACATGACGAAAACAGTGAAGAACAGGACGCCGGTGGGATTATTACGAAACGTAAGTATATGTTCGAACAAGTAGCTGCAGCAGCAGCCGATTCGGCGCTGTACGCTTGTCAGAACCAACGTTGCCCACAGAGCGAGGCGCCGATGGGATTCGCCGACAAGAACGCAAGAACAGAGCATGAGACGCAGTGCATTTACAGAGAAGCAGAGGATGAGAACATCGACACGCATTTGAAGAGCGTGGTGGAGTGGATGAACTGGGAGCTGTCGAGGGCGGAGCCAAGCAGCGAGGAGGGGAGGAGCGAAGACGCCGTAGATGGGTCCGGCAGCAGCACGGTGGAGAATTACGGGAGCAGGTACTGGAAGGGCGGCGTGGGAGAGGCGGCGGTGGATGAGGCGTTTGAGATGCATAGAGCGAGTATGGATTTGAATGCGAGTCCGGCGGAGGATTTGGGTGGTGGGCAACAAGATGCGACTTCCGTTTGGGATTTGATATATGAATGGGCTGAAGACTAA

Coding sequence (CDS)

ATGATGGTGGAATTCCATGGAGAAATCCGAAGCTCCGGCGATGGAGACGAGCAAGACAAGGAAGAGGAACTTAGCTACGATGATCTCAAGAAACGGATGTGGAAAGATAGACAGAGGATGAAGCAGCTGAAGGAGAAGCGGGATACTGAAGAACCTGAATCCACCGCTAGAGAAGAAGCCTCTCGCCGGAAAAAAATGGCCAGAGCTCAAGATTCGATCCTTAAGTACATGGATAAGATAATGGACGCTTGCAAGGCAAAAGGGTTTGTTTATGGGATCGTTCCGGAGAAGGGGAAACCAGTCACCGGCTCCTCGGAGAGCCTCCGGGAGTGGTGGCGGGACGACGTCCGGTTCGAACAAGATGCTCCCTTAGCCATAGCTCAATTCCTCCCGAAAGTTCTCGAAGAAACAGGGCTCGATTCGGAGTCGTTTTTGCACATGCTTCATGATTTACAGGACACAACATTGGGGTCGATTCTCTCTGCTTTGATTCAACACTGTATTCCGCCACAGAGGAAATTCCCACTTGAGAAGGGTTTAGCGCCGCCGTGGTGGCCGACGGGGAATGAGCTTTGGTGGGGTGAACAGGGTGCTGCTGGTGGACATGGCGTTCCGCCGTATAAAAAGCCTCACGATCTCAAAAAGGCATGGAAAATTAGTGTCTTGGCAGCAGTGATCAAACATATGTCCCCTGATTTGGACAATATGAGGAAATTGGTTCGACAATCGAAGAATTTACAAGCGAAAATGACGGCAAAGGAGACGCTTACGTGGGCTAAAGTCGTGAATCAAGAAGAATCCCTCTTGCAGCTAACAAAAAAAAGCCTCAAAATCACATCCGTCGAAGACGGACATGACGAAAACAGTGAAGAACAGGACGCCGGTGGGATTATTACGAAACGTAAGTATATGTTCGAACAAGTAGCTGCAGCAGCAGCCGATTCGGCGCTGTACGCTTGTCAGAACCAACGTTGCCCACAGAGCGAGGCGCCGATGGGATTCGCCGACAAGAACGCAAGAACAGAGCATGAGACGCAGTGCATTTACAGAGAAGCAGAGGATGAGAACATCGACACGCATTTGAAGAGCGTGGTGGAGTGGATGAACTGGGAGCTGTCGAGGGCGGAGCCAAGCAGCGAGGAGGGGAGGAGCGAAGACGCCGTAGATGGGTCCGGCAGCAGCACGGTGGAGAATTACGGGAGCAGGTACTGGAAGGGCGGCGTGGGAGAGGCGGCGGTGGATGAGGCGTTTGAGATGCATAGAGCGAGTATGGATTTGAATGCGAGTCCGGCGGAGGATTTGGGTGGTGGGCAACAAGATGCGACTTCCGTTTGGGATTTGATATATGAATGGGCTGAAGACTAA

Protein sequence

MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITKRKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTHLKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMHRASMDLNASPAEDLGGGQQDATSVWDLIYEWAED
Homology
BLAST of CmoCh16G007500 vs. ExPASy Swiss-Prot
Match: Q9LX16 (Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Arabidopsis thaliana OX=3702 GN=EIL4 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 6.5e-108
Identity = 217/402 (53.98%), Postives = 281/402 (69.90%), Query Frame = 0

Query: 19  DKEEELSYDDLKKRMWKDRQRM-KQLK-EKRDTEEPES--TAREEASRRKKMARAQDSIL 78
           D+EEE+SYDDLK+RMWKDR  M K+LK +KR + +  S  T R EASRRKKMAR+QDS+L
Sbjct: 15  DEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVL 74

Query: 79  KYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQFLPKV- 138
           KYM KIM+ CKAKGFVYGIVPEKGKP+TGSS+SLR WW+++V+F+Q+AP AI  +L    
Sbjct: 75  KYMMKIMEVCKAKGFVYGIVPEKGKPITGSSDSLRRWWKENVQFDQNAPDAITDYLALAA 134

Query: 139 ------LEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPT 198
                 L +    S S LHML +LQDTTLGS+LSAL+QHC+PPQR+FPLEKG+APPWWPT
Sbjct: 135 AAAAAELIDKSSSSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWPT 194

Query: 199 GNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQ 258
           G ELWWGEQGAA  HG PPY+KPHDL+K+WK+SVLAAVIKHMSP+L  +R+L RQSK+LQ
Sbjct: 195 GTELWWGEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSLQ 254

Query: 259 AKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITKRKYMFEQ 318
            KM AKET TW++V+NQEE+LL +  K LKI+       E+ ++Q++ G  +KRK     
Sbjct: 255 DKMMAKETDTWSRVLNQEEALLNI--KDLKIS-------EDQDDQESSG--SKRK----- 314

Query: 319 VAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAED-----ENIDTHLK 378
             +     ++Y CQN  CP+S+   GF DKN+RT HE QC+Y   ++     E   + L+
Sbjct: 315 SESMEPSKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGEYTSSLLE 374

Query: 379 SVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYW 405
           +V   +    S  +  +   R+ D  D    S   N+   +W
Sbjct: 375 TVPSIVTNSTSEDDYYNVSSRALDKRDDDDHSINGNWMEYFW 400

BLAST of CmoCh16G007500 vs. ExPASy Swiss-Prot
Match: Q9FJQ5 (ETHYLENE INSENSITIVE 3-like 5 protein OS=Arabidopsis thaliana OX=3702 GN=EIL5 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.4e-97
Identity = 233/544 (42.83%), Postives = 302/544 (55.51%), Query Frame = 0

Query: 14  DGDEQDKE----------EELSYDDLKKRMWKDRQRMKQLK-EKRDT-----EEPESTA- 73
           D DE D E          EE+SYDDLKKRMWKDR  M +LK +KRD        P S+  
Sbjct: 15  DYDEDDLEEDVDEFERFGEEISYDDLKKRMWKDRNLMCKLKQQKRDNLNSVISSPSSSTS 74

Query: 74  ------------REEASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGS 133
                       R EASRRKKMAR+QDS+LKYM KIM+ CKA+GFVYGIVPEKGKPVTGS
Sbjct: 75  ASSSSSSSVIVRRTEASRRKKMARSQDSVLKYMMKIMEVCKAQGFVYGIVPEKGKPVTGS 134

Query: 134 SESLREWWRDDVRFEQDAPLAIAQFL-----PKVLEETGLDSESFLHMLHDLQDTTLGSI 193
           S+SLR WW+++V+F+Q AP A++ +L       +     LD  S++HMLH+LQDTTLGS+
Sbjct: 135 SDSLRRWWKENVQFDQTAPNAVSDYLTLAAAQLISSNESLDPNSYIHMLHELQDTTLGSL 194

Query: 194 LSALIQHCIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAG-GHGVPPYKKPHDLKKAWK 253
           LSAL+QHC+PPQR+FPLEKGLAPPWWP G ELWWGEQGAA   HG PPY+KPHDL+KAWK
Sbjct: 195 LSALMQHCVPPQRRFPLEKGLAPPWWPNGTELWWGEQGAAAFEHGPPPYRKPHDLRKAWK 254

Query: 254 ISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKI 313
           +SVLAAVIKHMSP+L+ +R+L RQSK LQ KM AKET TW++V+NQEE+ L      LKI
Sbjct: 255 VSVLAAVIKHMSPNLERVRRLARQSKCLQDKMMAKETDTWSRVLNQEEARL----NRLKI 314

Query: 314 TSVEDGHDEN--------------------SEEQDAGGIITKRK---------------- 373
           +  ED   +                      ++Q+  G + K K                
Sbjct: 315 SDDEDEDRDQEQARFTCFDQEPSLNTCFIVGQDQEPLGSMRKDKRVDQEFSSNDCFLVAQ 374

Query: 374 -------------------YMFEQ-----------VAAAAADSALYACQNQRCPQSEAPM 433
                              ++ +Q           V   A  S +Y CQN  CP S+  +
Sbjct: 375 DQEPRKGKKADQEWSPNSCFLVDQEPLGNKRKGEFVEKEAMLSNVYTCQNSSCPSSDVSL 434

Query: 434 GFADKNARTEHETQCIYREAEDEN------IDTHLKSVVEWMNWELSRAEPSSEEGRSED 448
           GF DKN RT HE +C+Y   E  N       D  ++S+        +  +  S   ++ED
Sbjct: 435 GFVDKNLRTGHEIECLYGTPELVNQSSGGGSDGFVRSIT-------TSDDDYSASSKAED 494

BLAST of CmoCh16G007500 vs. ExPASy Swiss-Prot
Match: Q9SLH0 (ETHYLENE INSENSITIVE 3-like 1 protein OS=Arabidopsis thaliana OX=3702 GN=EIL1 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 3.1e-86
Identity = 178/395 (45.06%), Postives = 239/395 (60.51%), Query Frame = 0

Query: 21  EEELSYDDLKKRMWKDRQRMKQLKE-----KRDTEEPESTAREEASRRKKMARAQDSILK 80
           ++E+  D+L+KRMW+D+ R+K+LKE     K   +  +    +E +RRKKM+RAQD ILK
Sbjct: 43  DDEMDVDELEKRMWRDKMRLKRLKEQQSKCKEGVDGSKQRQSQEQARRKKMSRAQDGILK 102

Query: 81  YMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQFLPKVLE 140
           YM K+M+ CKA+GFVYGI+PEKGKPVTG+S++LREWW+D VRF+++ P AIA++  +   
Sbjct: 103 YMLKMMEVCKAQGFVYGIIPEKGKPVTGASDNLREWWKDKVRFDRNGPAAIAKYQSENNI 162

Query: 141 ETGLDSESFL-----HMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPTGNE 200
             G +  + L     H L +LQDTTLGS+LSAL+QHC PPQR+FPLEKG++PPWWP GNE
Sbjct: 163 SGGSNDCNSLVGPTPHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVSPPWWPNGNE 222

Query: 201 LWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKM 260
            WW + G     G PPYKKPHDLKKAWK+ VL AVIKHMSPD+  +RKLVRQSK LQ KM
Sbjct: 223 EWWPQLGLPNEQGPPPYKKPHDLKKAWKVGVLTAVIKHMSPDIAKIRKLVRQSKCLQDKM 282

Query: 261 TAKETLTWAKVVNQEE---------------SLLQLTKKSLKITSVEDGHDENSEEQDAG 320
           TAKE+ TW  ++NQEE               S   L   SL I    +   E  E++  G
Sbjct: 283 TAKESATWLAIINQEEVVARELYPESCPPLSSSSSLGSGSLLINDCSEYDVEGFEKEQHG 342

Query: 321 GIITKRK-------------------YMFEQVAAAAAD--------------------SA 352
             + +RK                   +  ++  A   +                    SA
Sbjct: 343 FDVEERKPEIVMMHPLASFGVAKMQHFPIKEEVATTVNLEFTRKRKQNNDMNVMVMDRSA 402

BLAST of CmoCh16G007500 vs. ExPASy Swiss-Prot
Match: Q8W3L9 (Protein ETHYLENE-INSENSITIVE 3-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=EIL2 PE=2 SV=2)

HSP 1 Score: 308.9 bits (790), Expect = 9.4e-83
Identity = 180/418 (43.06%), Postives = 245/418 (58.61%), Query Frame = 0

Query: 5   FHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEE-PESTA------- 64
           F  E  S GD D+ D ++    ++L++RMW+DR R K+LKE + +    ES A       
Sbjct: 59  FAAEDESDGDDDDDDDDDVDDIEELERRMWRDRVRHKRLKELQQSRAGRESRAGDAGGGG 118

Query: 65  -----REEASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREW 124
                 +E +RRKKM+RAQD ILKYM K+M+ C A+GFVYGI+PEKGKPV+G+S++LR W
Sbjct: 119 RQQRQSQEQARRKKMSRAQDGILKYMLKMMEVCNAQGFVYGIIPEKGKPVSGASDNLRSW 178

Query: 125 WRDDVRFEQDAPLAIAQF-----LPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQH 184
           W++ VRF+++ P AIA++     +P    + G  + +  H LH+LQDTTLGS+LSAL+QH
Sbjct: 179 WKEKVRFDRNGPAAIAKYQADNAVPGCDGDAGGAAPAGPHSLHELQDTTLGSLLSALMQH 238

Query: 185 CIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVI 244
           C PPQR+FPLEKG+ PPWWP G+E WW E G     G PPYKKPHDLKKAWK++VL AVI
Sbjct: 239 CDPPQRRFPLEKGVPPPWWPEGSEAWWPEAGVPKELGPPPYKKPHDLKKAWKVAVLTAVI 298

Query: 245 KHMSPDLDNMRKLVRQSKNLQAKMTAKETLTWAKVVNQEESL-LQLTKKSLK-------- 304
           KHMSPD+D +R+LVRQSK LQ KMTAKE +TW  V+ QEE L L+L   +L         
Sbjct: 299 KHMSPDVDKVRRLVRQSKCLQDKMTAKEIVTWLAVLKQEEDLYLKLHPGALPPPLSAASF 358

Query: 305 ITSVEDGHDENSEEQDAGG-----------------------IITKRKYMF--------- 353
             SV   +D    + D  G                        ++  K++          
Sbjct: 359 NASVSGEYDVEGVDGDEAGNNNLQKAQNDATAFMDLTTTMDAALSNNKFLIMPLMKEEAI 418

BLAST of CmoCh16G007500 vs. ExPASy Swiss-Prot
Match: O24606 (Protein ETHYLENE INSENSITIVE 3 OS=Arabidopsis thaliana OX=3702 GN=EIN3 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.7e-82
Identity = 174/388 (44.85%), Postives = 231/388 (59.54%), Query Frame = 0

Query: 21  EEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAR----EEASRRKKMARAQDSILKY 80
           ++E+  D+L++RMW+D+ R+K+LKE+   +E    A+    +E +RRKKM+RAQD ILKY
Sbjct: 43  DDEIDVDELERRMWRDKMRLKRLKEQDKGKEGVDAAKQRQSQEQARRKKMSRAQDGILKY 102

Query: 81  MDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQF-----LP 140
           M K+M+ CKA+GFVYGI+PE GKPVTG+S++LREWW+D VRF+++ P AI ++     +P
Sbjct: 103 MLKMMEVCKAQGFVYGIIPENGKPVTGASDNLREWWKDKVRFDRNGPAAITKYQAENNIP 162

Query: 141 KVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPTGNEL 200
            + E       +  H L +LQDTTLGS+LSAL+QHC PPQR+FPLEKG+ PPWWP G E 
Sbjct: 163 GIHEGNNPIGPT-PHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVPPPWWPNGKED 222

Query: 201 WWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKMT 260
           WW + G     G  PYKKPHDLKKAWK+ VL AVIKHM PD+  +RKLVRQSK LQ KMT
Sbjct: 223 WWPQLGLPKDQGPAPYKKPHDLKKAWKVGVLTAVIKHMFPDIAKIRKLVRQSKCLQDKMT 282

Query: 261 AKETLTWAKVVNQEESLL-QLTKKSLKITSVEDG-------------------------- 320
           AKE+ TW  ++NQEESL  +L  +S    S+  G                          
Sbjct: 283 AKESATWLAIINQEESLARELYPESCPPLSLSGGSCSLLMNDCSQYDVEGFEKESHYEVE 342

Query: 321 -------------------HD-ENSEEQDAGGIITKRKYMFEQVAAAAADSALYACQNQR 353
                              HD    EE  AG     RK    +      D  ++ C+N  
Sbjct: 343 ELKPEKVMNSSNFGMVAKMHDFPVKEEVPAGNSEFMRKRKPNRDLNTIMDRTVFTCENLG 402

BLAST of CmoCh16G007500 vs. ExPASy TrEMBL
Match: A0A6J1EN74 (putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita moschata OX=3662 GN=LOC111436029 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.8e-260
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60
           MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA
Sbjct: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60

Query: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120
           SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ
Sbjct: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120

Query: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180
           DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL
Sbjct: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180

Query: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240
           APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV
Sbjct: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240

Query: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300
           RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK
Sbjct: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300

Query: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360
           RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH
Sbjct: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360

Query: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420
           LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH
Sbjct: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420

Query: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
           RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED
Sbjct: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 454

BLAST of CmoCh16G007500 vs. ExPASy TrEMBL
Match: A0A6J1J695 (putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita maxima OX=3661 GN=LOC111482971 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.4e-244
Identity = 429/454 (94.49%), Postives = 439/454 (96.70%), Query Frame = 0

Query: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60
           MMVEFHGEIR SGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEK DTE+PES AREEA
Sbjct: 1   MMVEFHGEIRCSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKWDTEKPESAAREEA 60

Query: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120
           SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ
Sbjct: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120

Query: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180
           DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL
Sbjct: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180

Query: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240
           APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV
Sbjct: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240

Query: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300
           RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITS ED H EN EEQ AGGII+K
Sbjct: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSDEDEHGENREEQYAGGIISK 300

Query: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360
           RK +FEQVAAAAADSALYACQNQRCPQSEA MGF DK ARTE+ETQCIYREAED++IDTH
Sbjct: 301 RKCIFEQVAAAAADSALYACQNQRCPQSEATMGFTDKKARTENETQCIYREAEDQSIDTH 360

Query: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420
           LKSVVEWMNWELSRAEPSS+EGRSEDAVDGSGSSTVENYGS YWKGGVGEA+VDEAFEMH
Sbjct: 361 LKSVVEWMNWELSRAEPSSDEGRSEDAVDGSGSSTVENYGSSYWKGGVGEASVDEAFEMH 420

Query: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
           R SMDLNASPA+ LGGGQ+DATSVWDLIYEWAED
Sbjct: 421 RESMDLNASPAKVLGGGQEDATSVWDLIYEWAED 454

BLAST of CmoCh16G007500 vs. ExPASy TrEMBL
Match: A0A6J1C3X1 (putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Momordica charantia OX=3673 GN=LOC111008154 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.5e-197
Identity = 361/480 (75.21%), Postives = 401/480 (83.54%), Query Frame = 0

Query: 1   MMVEFHGEIR-SSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREE 60
           MMVEFHGEIR   GD +EQ++EEE+SYDDLKKRMWKDRQR+K++KE RD+EEP+S AREE
Sbjct: 1   MMVEFHGEIRPHDGDNEEQEEEEEISYDDLKKRMWKDRQRLKKMKETRDSEEPDSAAREE 60

Query: 61  ASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFE 120
           ASRRKKMARAQDSILKYMDKIM+ACKA+GFVYGIVPEKGKPVTGSSESLREWWRDDVRFE
Sbjct: 61  ASRRKKMARAQDSILKYMDKIMEACKAQGFVYGIVPEKGKPVTGSSESLREWWRDDVRFE 120

Query: 121 QDAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKG 180
           QDAP+AIA++LPKV+EETGLD+ SFLH+LHDLQDTTLGSILSAL+QHC+PPQRKFPLEKG
Sbjct: 121 QDAPIAIAEYLPKVIEETGLDTASFLHLLHDLQDTTLGSILSALMQHCMPPQRKFPLEKG 180

Query: 181 LAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKL 240
           LAPPWWPTG E+WWGEQGAAGGHGVPPYKKPHDLKKAWKIS+LAAVIKHMSPD+DNMRKL
Sbjct: 181 LAPPWWPTGKEIWWGEQGAAGGHGVPPYKKPHDLKKAWKISLLAAVIKHMSPDMDNMRKL 240

Query: 241 VRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQD------ 300
           VRQSKNLQAKMTAKETLTW KVVNQEE+LL+LTKKSLKIT  E  H+EN  E++      
Sbjct: 241 VRQSKNLQAKMTAKETLTWTKVVNQEEALLELTKKSLKITGEE--HEENRAEEEETRAEE 300

Query: 301 ---AGGIIT------KRKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHE 360
               GG  T      KRK +F +    A D+ALY CQN+ CPQSE+ MGF DKNARTEHE
Sbjct: 301 LVGGGGGTTHFRGNEKRKCLFNR--ETAGDTALYTCQNKWCPQSESLMGFVDKNARTEHE 360

Query: 361 TQCIYRE---------AEDENIDTHLKSVVEWMNWELSRAEP-SSEEGRSEDAVDGSGSS 420
            QC+YR          +++  ID HLKSVVEWMN EL+RAEP SSEE R ED  DGS S 
Sbjct: 361 AQCVYRNNAVVEAGQFSDEHAIDNHLKSVVEWMNLELARAEPNSSEEARMEDNGDGSESR 420

Query: 421 TVENYGSRYWKGGVGEAAVDEAFEMHRASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
            VENYG+RYWK GVGEA VDEAFEMH  SMDLN SP ED  G  QDATS+WDL YEW E+
Sbjct: 421 AVENYGTRYWKAGVGEATVDEAFEMHSESMDLNVSPTED-EGCHQDATSIWDLRYEWTEE 475

BLAST of CmoCh16G007500 vs. ExPASy TrEMBL
Match: A0A6J1ITS9 (putative ETHYLENE INSENSITIVE 3-like 4 protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479871 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 3.2e-195
Identity = 364/493 (73.83%), Postives = 405/493 (82.15%), Query Frame = 0

Query: 1   MMVEFHGEI-RSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREE 60
           MMVEFHGEI   S DGDE ++EEE+SYDDLKKRMWKDRQR+K++KE RD EE E  AREE
Sbjct: 1   MMVEFHGEIPEDSHDGDE-EQEEEISYDDLKKRMWKDRQRLKKMKENRDHEEFEWAAREE 60

Query: 61  ASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFE 120
           ASRRKKM+RAQDSILKYMDKIMD+CKA+GFVYGIVPEKGKP+TGSSESLREWW+D VRFE
Sbjct: 61  ASRRKKMSRAQDSILKYMDKIMDSCKAQGFVYGIVPEKGKPITGSSESLREWWQDKVRFE 120

Query: 121 QDAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKG 180
           Q+APL IA  LP+V+EE GLD++SFLH+L +LQDTTLGSILSAL+QHCIPPQRKFPL+KG
Sbjct: 121 QNAPLNIANLLPRVIEEAGLDTKSFLHLLQELQDTTLGSILSALMQHCIPPQRKFPLDKG 180

Query: 181 LAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKL 240
           LAPPWWPTG ELWWGEQG AGG GVPPYKKPHDLKK+WKISVLAAVIKHMS DL NMRKL
Sbjct: 181 LAPPWWPTGKELWWGEQGVAGGQGVPPYKKPHDLKKSWKISVLAAVIKHMSLDLGNMRKL 240

Query: 241 VRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVED---------GHDENSE 300
           VRQSKNLQAKMTAKET+TW KVVNQEE+LLQLTK SLKITS E+         GH EN  
Sbjct: 241 VRQSKNLQAKMTAKETITWTKVVNQEEALLQLTKNSLKITSDEEDQEQYHQNRGH-ENRA 300

Query: 301 EQDAGGIITKRKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYR 360
           EQ  GGI+TKRK  FE+   A ADSA+YACQN+ CPQSEAP GFADKNARTEHETQC+YR
Sbjct: 301 EQGTGGIVTKRKCEFER--EATADSAVYACQNEWCPQSEAPKGFADKNARTEHETQCVYR 360

Query: 361 E------AEDENIDTHLKSVVEWMNWELSRAEPSSEEGRSED------------------ 420
                  + +++IDTHL SVVEWMNWELSRAEPSS E R +D                  
Sbjct: 361 ADGVGQFSNEQSIDTHLMSVVEWMNWELSRAEPSSGESRIDDGGYGSGSSTVDGSGYWKG 420

Query: 421 -----AVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMHRASMDLNASPAEDLGGGQQDA 455
                AVDGSG STVE+YGS +WKGG+GEAAVDEAFEMHR SMDLNASPA+DL   QQDA
Sbjct: 421 GIGEAAVDGSGCSTVEDYGSGFWKGGIGEAAVDEAFEMHRESMDLNASPADDL-QCQQDA 480

BLAST of CmoCh16G007500 vs. ExPASy TrEMBL
Match: A0A6J1IZH5 (putative ETHYLENE INSENSITIVE 3-like 4 protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479871 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 9.5e-195
Identity = 363/493 (73.63%), Postives = 404/493 (81.95%), Query Frame = 0

Query: 1   MMVEFHGEI-RSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREE 60
           MMVEFHGEI   S DGDE ++EEE+SYDDLKKRMWKDRQR+K++KE RD EE E  AREE
Sbjct: 1   MMVEFHGEIPEDSHDGDE-EQEEEISYDDLKKRMWKDRQRLKKMKENRDHEEFEWAAREE 60

Query: 61  ASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFE 120
           ASRRKKM+RAQDSILKYMDKIMD+CKA+GFVYGIVPEKGKP+TGSSESLREWW+D VRFE
Sbjct: 61  ASRRKKMSRAQDSILKYMDKIMDSCKAQGFVYGIVPEKGKPITGSSESLREWWQDKVRFE 120

Query: 121 QDAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKG 180
           Q+APL IA  LP+V+EE GLD++SFLH+L +LQDTTLGSILSAL+QHCIPPQRKFPL+KG
Sbjct: 121 QNAPLNIANLLPRVIEEAGLDTKSFLHLLQELQDTTLGSILSALMQHCIPPQRKFPLDKG 180

Query: 181 LAPPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKL 240
           LAPPWWPTG ELWWGEQG AGG GVPPYKKPHDLKK+WKISVLAAVIKHMS DL NMRKL
Sbjct: 181 LAPPWWPTGKELWWGEQGVAGGQGVPPYKKPHDLKKSWKISVLAAVIKHMSLDLGNMRKL 240

Query: 241 VRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVED---------GHDENSE 300
           VRQSKNLQAKMTAKET+TW KVVNQEE+LLQLTK SLKITS E+         GH EN  
Sbjct: 241 VRQSKNLQAKMTAKETITWTKVVNQEEALLQLTKNSLKITSDEEDQEQYHQNRGH-ENRA 300

Query: 301 EQDAGGIITKRKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYR 360
           EQ  GGI+TKRK  FE+   A ADSA+YACQN+ CPQSEAP GFADKNARTEHETQC+YR
Sbjct: 301 EQGTGGIVTKRKCEFER--EATADSAVYACQNEWCPQSEAPKGFADKNARTEHETQCVYR 360

Query: 361 E------AEDENIDTHLKSVVEWMNWELSRAEPSSEEGRSED------------------ 420
                  + +++IDTHL SVVEWMNWELSRAEPSS E R +D                  
Sbjct: 361 ADGVGQFSNEQSIDTHLMSVVEWMNWELSRAEPSSGESRIDDGGYGSGSSTVDGSGYWKG 420

Query: 421 -----AVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMHRASMDLNASPAEDLGGGQQDA 455
                 VDGSG STVE+YGS +WKGG+GEAAVDEAFEMHR SMDLNASPA+DL   QQDA
Sbjct: 421 GIGEVGVDGSGCSTVEDYGSGFWKGGIGEAAVDEAFEMHRESMDLNASPADDL-QCQQDA 480

BLAST of CmoCh16G007500 vs. NCBI nr
Match: XP_022929461.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucurbita moschata])

HSP 1 Score: 906.7 bits (2342), Expect = 7.8e-260
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60
           MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA
Sbjct: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60

Query: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120
           SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ
Sbjct: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120

Query: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180
           DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL
Sbjct: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180

Query: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240
           APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV
Sbjct: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240

Query: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300
           RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK
Sbjct: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300

Query: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360
           RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH
Sbjct: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360

Query: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420
           LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH
Sbjct: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420

Query: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
           RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED
Sbjct: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 454

BLAST of CmoCh16G007500 vs. NCBI nr
Match: KAG7015369.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 893.3 bits (2307), Expect = 8.9e-256
Identity = 447/454 (98.46%), Postives = 449/454 (98.90%), Query Frame = 0

Query: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60
           MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPES AREEA
Sbjct: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESAAREEA 60

Query: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120
           SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ
Sbjct: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120

Query: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180
           DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL
Sbjct: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180

Query: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240
           APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV
Sbjct: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240

Query: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300
           RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDEN EEQDAGGIITK
Sbjct: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENREEQDAGGIITK 300

Query: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360
           RK +FEQVAA AADSALYACQN+RCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH
Sbjct: 301 RKCIFEQVAAVAADSALYACQNRRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360

Query: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420
           LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH
Sbjct: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420

Query: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
           R SMDLNASPAEDLGGGQQDATSVWDLIYEWAED
Sbjct: 421 RVSMDLNASPAEDLGGGQQDATSVWDLIYEWAED 454

BLAST of CmoCh16G007500 vs. NCBI nr
Match: KAG6577286.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 447/454 (98.46%), Postives = 449/454 (98.90%), Query Frame = 0

Query: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60
           MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPES AREEA
Sbjct: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESAAREEA 60

Query: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120
           SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ
Sbjct: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120

Query: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180
           DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL
Sbjct: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180

Query: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240
           APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV
Sbjct: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240

Query: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300
           RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDEN EEQDAGGIITK
Sbjct: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENREEQDAGGIITK 300

Query: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360
           RK  FEQVAA AADSALYACQN+RCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH
Sbjct: 301 RKCTFEQVAAVAADSALYACQNRRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360

Query: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420
           LKSVVEWMNWELSRAEPSSEEGRS+DAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH
Sbjct: 361 LKSVVEWMNWELSRAEPSSEEGRSKDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420

Query: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
           RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED
Sbjct: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 454

BLAST of CmoCh16G007500 vs. NCBI nr
Match: XP_023553151.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.1 bits (2226), Expect = 2.2e-246
Identity = 434/454 (95.59%), Postives = 440/454 (96.92%), Query Frame = 0

Query: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60
           MMVEFHGEIRSSGD DEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPES AREEA
Sbjct: 1   MMVEFHGEIRSSGDEDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESAAREEA 60

Query: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120
           SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ
Sbjct: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120

Query: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180
           DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL
Sbjct: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180

Query: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240
           APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDL+NMRKLV
Sbjct: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLENMRKLV 240

Query: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300
           RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTK+SLKITS ED HDEN EEQDAGGIITK
Sbjct: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKRSLKITSDEDEHDENREEQDAGGIITK 300

Query: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360
           RK +FEQVAAAAA S LY CQN+RCPQSEAPMGF DKNARTEHETQCIYREAEDEN+DTH
Sbjct: 301 RKCIFEQVAAAAAVSTLYPCQNRRCPQSEAPMGFTDKNARTEHETQCIYREAEDENVDTH 360

Query: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420
           LKSVVEWMNWELSRAEPSS+EGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH
Sbjct: 361 LKSVVEWMNWELSRAEPSSDEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420

Query: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
           R SMDLNASPA DL G QQDATSVWDLIYEWAED
Sbjct: 421 RVSMDLNASPAADL-GAQQDATSVWDLIYEWAED 453

BLAST of CmoCh16G007500 vs. NCBI nr
Match: XP_022984791.1 (putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucurbita maxima])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 429/454 (94.49%), Postives = 439/454 (96.70%), Query Frame = 0

Query: 1   MMVEFHGEIRSSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAREEA 60
           MMVEFHGEIR SGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEK DTE+PES AREEA
Sbjct: 1   MMVEFHGEIRCSGDGDEQDKEEELSYDDLKKRMWKDRQRMKQLKEKWDTEKPESAAREEA 60

Query: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120
           SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ
Sbjct: 61  SRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQ 120

Query: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180
           DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL
Sbjct: 121 DAPLAIAQFLPKVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGL 180

Query: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240
           APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV
Sbjct: 181 APPWWPTGNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLV 240

Query: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITK 300
           RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITS ED H EN EEQ AGGII+K
Sbjct: 241 RQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKITSDEDEHGENREEQYAGGIISK 300

Query: 301 RKYMFEQVAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAEDENIDTH 360
           RK +FEQVAAAAADSALYACQNQRCPQSEA MGF DK ARTE+ETQCIYREAED++IDTH
Sbjct: 301 RKCIFEQVAAAAADSALYACQNQRCPQSEATMGFTDKKARTENETQCIYREAEDQSIDTH 360

Query: 361 LKSVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYWKGGVGEAAVDEAFEMH 420
           LKSVVEWMNWELSRAEPSS+EGRSEDAVDGSGSSTVENYGS YWKGGVGEA+VDEAFEMH
Sbjct: 361 LKSVVEWMNWELSRAEPSSDEGRSEDAVDGSGSSTVENYGSSYWKGGVGEASVDEAFEMH 420

Query: 421 RASMDLNASPAEDLGGGQQDATSVWDLIYEWAED 455
           R SMDLNASPA+ LGGGQ+DATSVWDLIYEWAED
Sbjct: 421 RESMDLNASPAKVLGGGQEDATSVWDLIYEWAED 454

BLAST of CmoCh16G007500 vs. TAIR 10
Match: AT5G10120.1 (Ethylene insensitive 3 family protein )

HSP 1 Score: 392.5 bits (1007), Expect = 4.6e-109
Identity = 217/402 (53.98%), Postives = 281/402 (69.90%), Query Frame = 0

Query: 19  DKEEELSYDDLKKRMWKDRQRM-KQLK-EKRDTEEPES--TAREEASRRKKMARAQDSIL 78
           D+EEE+SYDDLK+RMWKDR  M K+LK +KR + +  S  T R EASRRKKMAR+QDS+L
Sbjct: 15  DEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVL 74

Query: 79  KYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQFLPKV- 138
           KYM KIM+ CKAKGFVYGIVPEKGKP+TGSS+SLR WW+++V+F+Q+AP AI  +L    
Sbjct: 75  KYMMKIMEVCKAKGFVYGIVPEKGKPITGSSDSLRRWWKENVQFDQNAPDAITDYLALAA 134

Query: 139 ------LEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPT 198
                 L +    S S LHML +LQDTTLGS+LSAL+QHC+PPQR+FPLEKG+APPWWPT
Sbjct: 135 AAAAAELIDKSSSSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWPT 194

Query: 199 GNELWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQ 258
           G ELWWGEQGAA  HG PPY+KPHDL+K+WK+SVLAAVIKHMSP+L  +R+L RQSK+LQ
Sbjct: 195 GTELWWGEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSLQ 254

Query: 259 AKMTAKETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITKRKYMFEQ 318
            KM AKET TW++V+NQEE+LL +  K LKI+       E+ ++Q++ G  +KRK     
Sbjct: 255 DKMMAKETDTWSRVLNQEEALLNI--KDLKIS-------EDQDDQESSG--SKRK----- 314

Query: 319 VAAAAADSALYACQNQRCPQSEAPMGFADKNARTEHETQCIYREAED-----ENIDTHLK 378
             +     ++Y CQN  CP+S+   GF DKN+RT HE QC+Y   ++     E   + L+
Sbjct: 315 SESMEPSKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGEYTSSLLE 374

Query: 379 SVVEWMNWELSRAEPSSEEGRSEDAVDGSGSSTVENYGSRYW 405
           +V   +    S  +  +   R+ D  D    S   N+   +W
Sbjct: 375 TVPSIVTNSTSEDDYYNVSSRALDKRDDDDHSINGNWMEYFW 400

BLAST of CmoCh16G007500 vs. TAIR 10
Match: AT5G65100.1 (Ethylene insensitive 3 family protein )

HSP 1 Score: 358.2 bits (918), Expect = 9.6e-99
Identity = 233/544 (42.83%), Postives = 302/544 (55.51%), Query Frame = 0

Query: 14  DGDEQDKE----------EELSYDDLKKRMWKDRQRMKQLK-EKRDT-----EEPESTA- 73
           D DE D E          EE+SYDDLKKRMWKDR  M +LK +KRD        P S+  
Sbjct: 15  DYDEDDLEEDVDEFERFGEEISYDDLKKRMWKDRNLMCKLKQQKRDNLNSVISSPSSSTS 74

Query: 74  ------------REEASRRKKMARAQDSILKYMDKIMDACKAKGFVYGIVPEKGKPVTGS 133
                       R EASRRKKMAR+QDS+LKYM KIM+ CKA+GFVYGIVPEKGKPVTGS
Sbjct: 75  ASSSSSSSVIVRRTEASRRKKMARSQDSVLKYMMKIMEVCKAQGFVYGIVPEKGKPVTGS 134

Query: 134 SESLREWWRDDVRFEQDAPLAIAQFL-----PKVLEETGLDSESFLHMLHDLQDTTLGSI 193
           S+SLR WW+++V+F+Q AP A++ +L       +     LD  S++HMLH+LQDTTLGS+
Sbjct: 135 SDSLRRWWKENVQFDQTAPNAVSDYLTLAAAQLISSNESLDPNSYIHMLHELQDTTLGSL 194

Query: 194 LSALIQHCIPPQRKFPLEKGLAPPWWPTGNELWWGEQGAAG-GHGVPPYKKPHDLKKAWK 253
           LSAL+QHC+PPQR+FPLEKGLAPPWWP G ELWWGEQGAA   HG PPY+KPHDL+KAWK
Sbjct: 195 LSALMQHCVPPQRRFPLEKGLAPPWWPNGTELWWGEQGAAAFEHGPPPYRKPHDLRKAWK 254

Query: 254 ISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKMTAKETLTWAKVVNQEESLLQLTKKSLKI 313
           +SVLAAVIKHMSP+L+ +R+L RQSK LQ KM AKET TW++V+NQEE+ L      LKI
Sbjct: 255 VSVLAAVIKHMSPNLERVRRLARQSKCLQDKMMAKETDTWSRVLNQEEARL----NRLKI 314

Query: 314 TSVEDGHDEN--------------------SEEQDAGGIITKRK---------------- 373
           +  ED   +                      ++Q+  G + K K                
Sbjct: 315 SDDEDEDRDQEQARFTCFDQEPSLNTCFIVGQDQEPLGSMRKDKRVDQEFSSNDCFLVAQ 374

Query: 374 -------------------YMFEQ-----------VAAAAADSALYACQNQRCPQSEAPM 433
                              ++ +Q           V   A  S +Y CQN  CP S+  +
Sbjct: 375 DQEPRKGKKADQEWSPNSCFLVDQEPLGNKRKGEFVEKEAMLSNVYTCQNSSCPSSDVSL 434

Query: 434 GFADKNARTEHETQCIYREAEDEN------IDTHLKSVVEWMNWELSRAEPSSEEGRSED 448
           GF DKN RT HE +C+Y   E  N       D  ++S+        +  +  S   ++ED
Sbjct: 435 GFVDKNLRTGHEIECLYGTPELVNQSSGGGSDGFVRSIT-------TSDDDYSASSKAED 494

BLAST of CmoCh16G007500 vs. TAIR 10
Match: AT2G27050.1 (ETHYLENE-INSENSITIVE3-like 1 )

HSP 1 Score: 320.5 bits (820), Expect = 2.2e-87
Identity = 178/395 (45.06%), Postives = 239/395 (60.51%), Query Frame = 0

Query: 21  EEELSYDDLKKRMWKDRQRMKQLKE-----KRDTEEPESTAREEASRRKKMARAQDSILK 80
           ++E+  D+L+KRMW+D+ R+K+LKE     K   +  +    +E +RRKKM+RAQD ILK
Sbjct: 43  DDEMDVDELEKRMWRDKMRLKRLKEQQSKCKEGVDGSKQRQSQEQARRKKMSRAQDGILK 102

Query: 81  YMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQFLPKVLE 140
           YM K+M+ CKA+GFVYGI+PEKGKPVTG+S++LREWW+D VRF+++ P AIA++  +   
Sbjct: 103 YMLKMMEVCKAQGFVYGIIPEKGKPVTGASDNLREWWKDKVRFDRNGPAAIAKYQSENNI 162

Query: 141 ETGLDSESFL-----HMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPTGNE 200
             G +  + L     H L +LQDTTLGS+LSAL+QHC PPQR+FPLEKG++PPWWP GNE
Sbjct: 163 SGGSNDCNSLVGPTPHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVSPPWWPNGNE 222

Query: 201 LWWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKM 260
            WW + G     G PPYKKPHDLKKAWK+ VL AVIKHMSPD+  +RKLVRQSK LQ KM
Sbjct: 223 EWWPQLGLPNEQGPPPYKKPHDLKKAWKVGVLTAVIKHMSPDIAKIRKLVRQSKCLQDKM 282

Query: 261 TAKETLTWAKVVNQEE---------------SLLQLTKKSLKITSVEDGHDENSEEQDAG 320
           TAKE+ TW  ++NQEE               S   L   SL I    +   E  E++  G
Sbjct: 283 TAKESATWLAIINQEEVVARELYPESCPPLSSSSSLGSGSLLINDCSEYDVEGFEKEQHG 342

Query: 321 GIITKRK-------------------YMFEQVAAAAAD--------------------SA 352
             + +RK                   +  ++  A   +                    SA
Sbjct: 343 FDVEERKPEIVMMHPLASFGVAKMQHFPIKEEVATTVNLEFTRKRKQNNDMNVMVMDRSA 402

BLAST of CmoCh16G007500 vs. TAIR 10
Match: AT3G20770.1 (Ethylene insensitive 3 family protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-83
Identity = 174/388 (44.85%), Postives = 231/388 (59.54%), Query Frame = 0

Query: 21  EEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTAR----EEASRRKKMARAQDSILKY 80
           ++E+  D+L++RMW+D+ R+K+LKE+   +E    A+    +E +RRKKM+RAQD ILKY
Sbjct: 43  DDEIDVDELERRMWRDKMRLKRLKEQDKGKEGVDAAKQRQSQEQARRKKMSRAQDGILKY 102

Query: 81  MDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQF-----LP 140
           M K+M+ CKA+GFVYGI+PE GKPVTG+S++LREWW+D VRF+++ P AI ++     +P
Sbjct: 103 MLKMMEVCKAQGFVYGIIPENGKPVTGASDNLREWWKDKVRFDRNGPAAITKYQAENNIP 162

Query: 141 KVLEETGLDSESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPTGNEL 200
            + E       +  H L +LQDTTLGS+LSAL+QHC PPQR+FPLEKG+ PPWWP G E 
Sbjct: 163 GIHEGNNPIGPT-PHTLQELQDTTLGSLLSALMQHCDPPQRRFPLEKGVPPPWWPNGKED 222

Query: 201 WWGEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKMT 260
           WW + G     G  PYKKPHDLKKAWK+ VL AVIKHM PD+  +RKLVRQSK LQ KMT
Sbjct: 223 WWPQLGLPKDQGPAPYKKPHDLKKAWKVGVLTAVIKHMFPDIAKIRKLVRQSKCLQDKMT 282

Query: 261 AKETLTWAKVVNQEESLL-QLTKKSLKITSVEDG-------------------------- 320
           AKE+ TW  ++NQEESL  +L  +S    S+  G                          
Sbjct: 283 AKESATWLAIINQEESLARELYPESCPPLSLSGGSCSLLMNDCSQYDVEGFEKESHYEVE 342

Query: 321 -------------------HD-ENSEEQDAGGIITKRKYMFEQVAAAAADSALYACQNQR 353
                              HD    EE  AG     RK    +      D  ++ C+N  
Sbjct: 343 ELKPEKVMNSSNFGMVAKMHDFPVKEEVPAGNSEFMRKRKPNRDLNTIMDRTVFTCENLG 402

BLAST of CmoCh16G007500 vs. TAIR 10
Match: AT1G73730.1 (ETHYLENE-INSENSITIVE3-like 3 )

HSP 1 Score: 282.3 bits (721), Expect = 6.7e-76
Identity = 146/289 (50.52%), Postives = 200/289 (69.20%), Query Frame = 0

Query: 21  EEELSYDDLKKRMWKDRQRMKQLKEKRDTEEPESTARE------EASRRKKMARAQDSIL 80
           +EE+  DDL++RMWKDR R+K++KE++      +  +E      + ++RKKM+RAQD IL
Sbjct: 32  DEEIDADDLERRMWKDRVRLKRIKERQKAGSQGAQTKETPKKISDQAQRKKMSRAQDGIL 91

Query: 81  KYMDKIMDACKAKGFVYGIVPEKGKPVTGSSESLREWWRDDVRFEQDAPLAIAQFLPKVL 140
           KYM K+M+ CK +GFVYGI+PEKGKPV+GSS+++R WW++ V+F+++ P AIA++  + L
Sbjct: 92  KYMLKLMEVCKVRGFVYGIIPEKGKPVSGSSDNIRAWWKEKVKFDKNGPAAIAKYEEECL 151

Query: 141 EETGLD-SESFLHMLHDLQDTTLGSILSALIQHCIPPQRKFPLEKGLAPPWWPTGNELWW 200
                D + +   +L DLQD TLGS+LS+L+QHC PPQRK+PLEKG  PPWWPTGNE WW
Sbjct: 152 AFGKSDGNRNSQFVLQDLQDATLGSLLSSLMQHCDPPQRKYPLEKGTPPPWWPTGNEEWW 211

Query: 201 GEQGAAGGHGVPPYKKPHDLKKAWKISVLAAVIKHMSPDLDNMRKLVRQSKNLQAKMTAK 260
            + G       PPY+KPHDLKK WK+ VL AVI HM PD+  +++ VRQSK LQ KMTAK
Sbjct: 212 VKLGLPKSQS-PPYRKPHDLKKMWKVGVLTAVINHMLPDIAKIKRHVRQSKCLQDKMTAK 271

Query: 261 ETLTWAKVVNQEESLLQLTKKSLKITSVEDGHDENSEEQDAGGIITKRK 303
           E+  W  V+NQEESL+Q         S ++G+   +E    G    +RK
Sbjct: 272 ESAIWLAVLNQEESLIQQ-------PSSDNGNSNVTETHRRGNNADRRK 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LX166.5e-10853.98Putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FJQ51.4e-9742.83ETHYLENE INSENSITIVE 3-like 5 protein OS=Arabidopsis thaliana OX=3702 GN=EIL5 PE... [more]
Q9SLH03.1e-8645.06ETHYLENE INSENSITIVE 3-like 1 protein OS=Arabidopsis thaliana OX=3702 GN=EIL1 PE... [more]
Q8W3L99.4e-8343.06Protein ETHYLENE-INSENSITIVE 3-like 2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
O246062.7e-8244.85Protein ETHYLENE INSENSITIVE 3 OS=Arabidopsis thaliana OX=3702 GN=EIN3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EN743.8e-260100.00putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1J6958.4e-24494.49putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1C3X14.5e-19775.21putative ETHYLENE INSENSITIVE 3-like 4 protein OS=Momordica charantia OX=3673 GN... [more]
A0A6J1ITS93.2e-19573.83putative ETHYLENE INSENSITIVE 3-like 4 protein isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1IZH59.5e-19573.63putative ETHYLENE INSENSITIVE 3-like 4 protein isoform X1 OS=Cucurbita maxima OX... [more]
Match NameE-valueIdentityDescription
XP_022929461.17.8e-260100.00putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucurbita moschata][more]
KAG7015369.18.9e-25698.46putative ETHYLENE INSENSITIVE 3-like 4 protein, partial [Cucurbita argyrosperma ... [more]
KAG6577286.11.5e-25598.46putative ETHYLENE INSENSITIVE 3-like 4 protein, partial [Cucurbita argyrosperma ... [more]
XP_023553151.12.2e-24695.59putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucurbita pepo subsp. pepo][more]
XP_022984791.11.7e-24394.49putative ETHYLENE INSENSITIVE 3-like 4 protein [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G10120.14.6e-10953.98Ethylene insensitive 3 family protein [more]
AT5G65100.19.6e-9942.83Ethylene insensitive 3 family protein [more]
AT2G27050.12.2e-8745.06ETHYLENE-INSENSITIVE3-like 1 [more]
AT3G20770.11.9e-8344.85Ethylene insensitive 3 family protein [more]
AT1G73730.16.7e-7650.52ETHYLENE-INSENSITIVE3-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 26..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 375..394
NoneNo IPR availablePANTHERPTHR33305:SF29ETHYLENE INSENSITIVE 3-LIKE 5 PROTEINcoord: 16..450
IPR006957Ethylene insensitive 3PFAMPF04873EIN3coord: 27..266
e-value: 2.3E-109
score: 364.6
IPR006957Ethylene insensitive 3PANTHERPTHR33305ETHYLENE INSENSITIVE 3-LIKE 2 PROTEINcoord: 16..450
IPR023278Ethylene insensitive 3-like protein, DNA-binding domain superfamilyGENE3D1.10.3180.10coord: 138..276
e-value: 9.3E-65
score: 218.4
coord: 55..135
e-value: 1.9E-6
score: 29.4
IPR023278Ethylene insensitive 3-like protein, DNA-binding domain superfamilySUPERFAMILY116768DNA-binding domain of EIN3-likecoord: 145..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G007500.1CmoCh16G007500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009873 ethylene-activated signaling pathway
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity