CmoCh16G006250 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G006250
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMembrane-associated kinase regulator 5
LocationCmo_Chr16: 3075880 .. 3078064 (+)
RNA-Seq ExpressionCmoCh16G006250
SyntenyCmoCh16G006250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAACGTAAAAATGTTATCTTTCTTCTTTGGTAAAGAAAGGTATTTCTTTACCAACTCTGTGACACACTCCCATTTTCAGACCTGCAAAGTGCAACAACCATTACCATCGTCCTTCTCCTGAAACCTCTGACTGACCTCGACCTCAAGGGACCTCCTTTTCCCCCTTATCTTTTACTTGCTTCTCTTTTCCTTTTTACCCCCTTCTTCTCTAAACCTAACCCATTCCTCTATTCTGGCTCTCCCAACCATGGAAAGCTTCAGCTTGCTCAAATACTGGAGAGGGGGCGGCGGTGCTACTTCTGCTGTTGCTGCTGCTGCCTCTGCTGATTTTGCTTTTAATGGAAATTCCTCTGCTGCCGGCGCTGTCAGTGTTGTCACCGCCGTGCCGCAGAACCAGGCGGAAAGTGATGAGGAGAATGATGAGGATGATGGGCCGTTCTTTGATTTGGAGTTTGCTGTTCCTGATGAGGATGAGAGTCGAAAACAGGGGGGAAATGATGAGGGGAGGAGAAACTGTGCTGATGGAAGTGAAGAAGAGGAAGAAGATGATGATGATGATGAAGAAGAAGATGGGGATTCGGATGGTGAGAGAGAGTTCAACTTCACGCTGTCATCTGGTTCTAGCAACGAACATTCCGATGCCAATCTCGCGTTTTCTCCTTCTGATGACTTGTTTTTCAAGGGAAAGCTTGTCCCAGTTGAAGCCTCCTCCAATGGCGGTTCTGAACCAAACGTCAGGCCGCAGCTTCGTGTCACATTGTTGAAATCAGCTGCCAAGTTTAGGGTATTCATGTCGGGTCTCAAGAAGTCGAAATCGAACGGGGAAAAGAAACCAGAGACGGATGGATCTGTGGGGGAAATGACCAAAACTGAGATGAAGGAAGAGACGACGGAAATCCCACCGGAGAAAAACGAGAACCAACCGGTAAAAGAACAGAGCCAGACGAAAAGTAAGCTTTTCGCTGTGAAACTTAAGGTGGAGGAAGTTCCGATCATGTCTCTGTTTACCAGAGACAACAGTTCAAGAGGCTCCGGAGGAACTACCAGCAGCAACAACAACAACAACAACAACAAACCCCAGAAACAAAGCTCCGATGAACTAACTTCGGAGGAAAATCGATTCTCCAAAGACGTAATAATGCAAAAGTACTTGAAGATGGTGAAGCCTCTGTACATTCGGGTTTCAAGGAGATACGGCGAGAAGTTCAGGCTATCCGGGCAGCTTATTTTGGGGGCAGCCGGAGCCAAGTCCGGTTCACCACCACCCACCTCCCCGCCGTTGGAGAAACCCGTGACAGAGACAGAGACACAGACACAGACAGAAGGTGTGCAAGTGCAAGCCCCAACAACCACCGCCAACAGCAAGGGCAGGACCATGCCGGCTAAGCTAAGAGTAGTTTGCAAGCATTTGGGGAAAAGTCGGTCAGCTTCCTCCGCCGTAGCAGCGGCGCCACCAGGGATGGCTCCCTCGAGAAGGGACGATTCGCTTATCCAGCAACAAGACGGAATCCAGAGCGCGATTCTCCATTGCAAGAGATCCTTCAACGCATCTCGAGGTGAATAAAACTGTCAACTATTCCGTTACTTGTATTAAAAATTAACCCTTTTCTTCCTTCTTCTTCTTCTTCTTCATTAAAACGGATAATTTCTTTCAATGACAGATTCAGAGTCTTCCCTGCTATCAAGTCAGTAAGCGACACTCTGCGTGAGAAACCTTCAGATTTAACAGAAAAACAATGATCAGATTAAAGCCGACAGAAACGGCGGGAAATTTTTGGTCGATATCCTGAAGATTTCTCTCGCTTTATATATAAATATGTGTAGTATATGTAACATATGCAGCTGTGTTGGGTTAGGATTGAATTCCTGGGGTGGCTTCTAAGTAAAAGTTTAATCTTTCCATTGAAGGGAAAAGAGAAGAGGGAAGAAATATTGGATTGTATGCGTTTATTGTAACCAGTAGAAAGATACACACAATCCAAGAGCAGTTGTCCCTAAGTATGTGATTTTTCCTTTGCCTATCCCAATTGGAGCTGAAAAACCACTGAGCTCAAGTGGGTGCTATGAAACTGTGTTGCTTTTTTTGGCTTTTGAGATTGGTAAAGGATGTGGGTCTTAACTTAGGCTGTTCTCTGTGGCAAAAAGTGGTGTGTTTAGTGGTCAGAGAAGTGGGGAAAAGTGGG

mRNA sequence

ATAAACGTAAAAATGTTATCTTTCTTCTTTGGTAAAGAAAGGTATTTCTTTACCAACTCTGTGACACACTCCCATTTTCAGACCTGCAAAGTGCAACAACCATTACCATCGTCCTTCTCCTGAAACCTCTGACTGACCTCGACCTCAAGGGACCTCCTTTTCCCCCTTATCTTTTACTTGCTTCTCTTTTCCTTTTTACCCCCTTCTTCTCTAAACCTAACCCATTCCTCTATTCTGGCTCTCCCAACCATGGAAAGCTTCAGCTTGCTCAAATACTGGAGAGGGGGCGGCGGTGCTACTTCTGCTGTTGCTGCTGCTGCCTCTGCTGATTTTGCTTTTAATGGAAATTCCTCTGCTGCCGGCGCTGTCAGTGTTGTCACCGCCGTGCCGCAGAACCAGGCGGAAAGTGATGAGGAGAATGATGAGGATGATGGGCCGTTCTTTGATTTGGAGTTTGCTGTTCCTGATGAGGATGAGAGTCGAAAACAGGGGGGAAATGATGAGGGGAGGAGAAACTGTGCTGATGGAAGTGAAGAAGAGGAAGAAGATGATGATGATGATGAAGAAGAAGATGGGGATTCGGATGGTGAGAGAGAGTTCAACTTCACGCTGTCATCTGGTTCTAGCAACGAACATTCCGATGCCAATCTCGCGTTTTCTCCTTCTGATGACTTGTTTTTCAAGGGAAAGCTTGTCCCAGTTGAAGCCTCCTCCAATGGCGGTTCTGAACCAAACGTCAGGCCGCAGCTTCGTGTCACATTGTTGAAATCAGCTGCCAAGTTTAGGGTATTCATGTCGGGTCTCAAGAAGTCGAAATCGAACGGGGAAAAGAAACCAGAGACGGATGGATCTGTGGGGGAAATGACCAAAACTGAGATGAAGGAAGAGACGACGGAAATCCCACCGGAGAAAAACGAGAACCAACCGGTAAAAGAACAGAGCCAGACGAAAAGTAAGCTTTTCGCTGTGAAACTTAAGGTGGAGGAAGTTCCGATCATGTCTCTGTTTACCAGAGACAACAGTTCAAGAGGCTCCGGAGGAACTACCAGCAGCAACAACAACAACAACAACAACAAACCCCAGAAACAAAGCTCCGATGAACTAACTTCGGAGGAAAATCGATTCTCCAAAGACGTAATAATGCAAAAGTACTTGAAGATGGTGAAGCCTCTGTACATTCGGGTTTCAAGGAGATACGGCGAGAAGTTCAGGCTATCCGGGCAGCTTATTTTGGGGGCAGCCGGAGCCAAGTCCGGTTCACCACCACCCACCTCCCCGCCGTTGGAGAAACCCGTGACAGAGACAGAGACACAGACACAGACAGAAGGTGTGCAAGTGCAAGCCCCAACAACCACCGCCAACAGCAAGGGCAGGACCATGCCGGCTAAGCTAAGAGTAGTTTGCAAGCATTTGGGGAAAAGTCGGTCAGCTTCCTCCGCCGTAGCAGCGGCGCCACCAGGGATGGCTCCCTCGAGAAGGGACGATTCGCTTATCCAGCAACAAGACGGAATCCAGAGCGCGATTCTCCATTGCAAGAGATCCTTCAACGCATCTCGAGATTCAGAGTCTTCCCTGCTATCAAGTCAGTAAGCGACACTCTGCGTGAGAAACCTTCAGATTTAACAGAAAAACAATGATCAGATTAAAGCCGACAGAAACGGCGGGAAATTTTTGGTCGATATCCTGAAGATTTCTCTCGCTTTATATATAAATATGTGTAGTATATGTAACATATGCAGCTGTGTTGGGTTAGGATTGAATTCCTGGGGTGGCTTCTAAGTAAAAGTTTAATCTTTCCATTGAAGGGAAAAGAGAAGAGGGAAGAAATATTGGATTGTATGCGTTTATTGTAACCAGTAGAAAGATACACACAATCCAAGAGCAGTTGTCCCTAAGTATGTGATTTTTCCTTTGCCTATCCCAATTGGAGCTGAAAAACCACTGAGCTCAAGTGGGTGCTATGAAACTGTGTTGCTTTTTTTGGCTTTTGAGATTGGTAAAGGATGTGGGTCTTAACTTAGGCTGTTCTCTGTGGCAAAAAGTGGTGTGTTTAGTGGTCAGAGAAGTGGGGAAAAGTGGG

Coding sequence (CDS)

ATGGAAAGCTTCAGCTTGCTCAAATACTGGAGAGGGGGCGGCGGTGCTACTTCTGCTGTTGCTGCTGCTGCCTCTGCTGATTTTGCTTTTAATGGAAATTCCTCTGCTGCCGGCGCTGTCAGTGTTGTCACCGCCGTGCCGCAGAACCAGGCGGAAAGTGATGAGGAGAATGATGAGGATGATGGGCCGTTCTTTGATTTGGAGTTTGCTGTTCCTGATGAGGATGAGAGTCGAAAACAGGGGGGAAATGATGAGGGGAGGAGAAACTGTGCTGATGGAAGTGAAGAAGAGGAAGAAGATGATGATGATGATGAAGAAGAAGATGGGGATTCGGATGGTGAGAGAGAGTTCAACTTCACGCTGTCATCTGGTTCTAGCAACGAACATTCCGATGCCAATCTCGCGTTTTCTCCTTCTGATGACTTGTTTTTCAAGGGAAAGCTTGTCCCAGTTGAAGCCTCCTCCAATGGCGGTTCTGAACCAAACGTCAGGCCGCAGCTTCGTGTCACATTGTTGAAATCAGCTGCCAAGTTTAGGGTATTCATGTCGGGTCTCAAGAAGTCGAAATCGAACGGGGAAAAGAAACCAGAGACGGATGGATCTGTGGGGGAAATGACCAAAACTGAGATGAAGGAAGAGACGACGGAAATCCCACCGGAGAAAAACGAGAACCAACCGGTAAAAGAACAGAGCCAGACGAAAAGTAAGCTTTTCGCTGTGAAACTTAAGGTGGAGGAAGTTCCGATCATGTCTCTGTTTACCAGAGACAACAGTTCAAGAGGCTCCGGAGGAACTACCAGCAGCAACAACAACAACAACAACAACAAACCCCAGAAACAAAGCTCCGATGAACTAACTTCGGAGGAAAATCGATTCTCCAAAGACGTAATAATGCAAAAGTACTTGAAGATGGTGAAGCCTCTGTACATTCGGGTTTCAAGGAGATACGGCGAGAAGTTCAGGCTATCCGGGCAGCTTATTTTGGGGGCAGCCGGAGCCAAGTCCGGTTCACCACCACCCACCTCCCCGCCGTTGGAGAAACCCGTGACAGAGACAGAGACACAGACACAGACAGAAGGTGTGCAAGTGCAAGCCCCAACAACCACCGCCAACAGCAAGGGCAGGACCATGCCGGCTAAGCTAAGAGTAGTTTGCAAGCATTTGGGGAAAAGTCGGTCAGCTTCCTCCGCCGTAGCAGCGGCGCCACCAGGGATGGCTCCCTCGAGAAGGGACGATTCGCTTATCCAGCAACAAGACGGAATCCAGAGCGCGATTCTCCATTGCAAGAGATCCTTCAACGCATCTCGAGATTCAGAGTCTTCCCTGCTATCAAGTCAGTAA

Protein sequence

MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDEDDGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDGEREFNFTLSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKSKLFAVKLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNNNNNKPQKQSSDELTSEENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTETETQTQTEGVQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRDDSLIQQQDGIQSAILHCKRSFNASRDSESSLLSSQ
Homology
BLAST of CmoCh16G006250 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.1e-54
Identity = 183/460 (39.78%), Postives = 247/460 (53.70%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGATSAV---AAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEEN 60
           ME+FSLL YW+  GGA + +   + ++S+         + G  ++VT+V + + E  +EN
Sbjct: 1   MEAFSLLNYWKNSGGAVTFLPHSSESSSSSLCRYSAGESIGTTTIVTSVTETEDEEADEN 60

Query: 61  DEDDGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDGEREF 120
           D D+GPFFDLEFA+P E+E    G   EG  +C DG                      EF
Sbjct: 61  D-DEGPFFDLEFALPAEEEDTDGGVGREGDSDCTDGG--------------------CEF 120

Query: 121 NFTLSSGSSNEHSDANLAFSPSDDLFFKGKLV-----PVEASSNGGSEPNVRPQLRVTLL 180
            FTLSS S  E     +     DD+FFK  +V     P  +     S      QL  ++L
Sbjct: 121 KFTLSSCSGGED---RVDPDSLDDVFFKETIVEEVEPPTSSEQQTCSVKAPAAQLSASIL 180

Query: 181 KSAAKFRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQ 240
           K+A K RVFM G+KKSK    K     GS       E  ++ T  PP  +    +  +SQ
Sbjct: 181 KTATKLRVFMLGMKKSKLIQAKSEANFGS-------EDLDKQTHPPPPLS----LSPESQ 240

Query: 241 TKSKLFAVKLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNNNNNKPQKQSSDELTSEENRF 300
            KS +  V LKVEEVPI+SLF+R+NSS+ S  ++SS+++++++  ++  ++ + S+E RF
Sbjct: 241 QKSTV-TVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSSSSSLKKQNGNESVVSDEKRF 300

Query: 301 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTET 360
              V+MQKYLK VKPLYIRVSRRYGEK R SGQL              T+P        +
Sbjct: 301 ---VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL--------------TAP--------S 360

Query: 361 ETQTQTEGVQVQAPTTTANSKGR--TMPAKLRVVCKHLGKSRSA-------SSAVAAAPP 420
             ++  E       T T N  G    +PA L+VV KHLGKSRS+       SS  A    
Sbjct: 361 SARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTATVTT 399

Query: 421 GMAPSRRDDSLIQQQDGIQSAILHCKRSFNASRDSESSLL 444
                RRDDSL+QQQD IQSAILHCKRSFN+SRD + S+L
Sbjct: 421 QSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDKDPSVL 399

BLAST of CmoCh16G006250 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.8e-04
Identity = 77/284 (27.11%), Postives = 132/284 (46.48%), Query Frame = 0

Query: 170 TLLKSAAKFRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKE 229
           T +K   + R+  S +    + G + P+ D   GE +  E+K   ++    K   +  + 
Sbjct: 15  TTIKPRREIRISESAV--DSTTGSEDPDLDLCEGEDSFFELKISLSDF---KTPKEKQRL 74

Query: 230 QSQTKSKLFAVKLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNN-----NNNKPQKQSSDE 289
           ++ T +  ++V  K + +P + + ++   S     TT   +       +  K +K ++ E
Sbjct: 75  ETTTTTTTYSVSNKSKVLPFVDISSKPQQS----PTTLLKSGQKFRAFSFKKSEKSTTTE 134

Query: 290 LTSEENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPP 349
              EEN  +   +  +          R +       +    +   +   +  S       
Sbjct: 135 KKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQQTDDTMFDDSVSKRFFS------- 194

Query: 350 LEKPVTETETQTQTEGVQVQAPTT---TANSKGRTMPAKLRVVCKHLGKSRSASSAVAAA 409
           L KP+    T+ Q+    + +PT+   T   +   +P+ +R V + LGKSRSAS+A+   
Sbjct: 195 LIKPLYTKSTKKQSSST-ITSPTSSPATREKQRSNIPSGMRSVRRQLGKSRSASAAIG-- 254

Query: 410 PPGMAPSRR-DDSLIQQQDGIQSAILHCKRSFNASRDSESSLLS 445
             GM+P+ R D+SL  QQDGIQSAILHCK+SF+ SR  ESSLLS
Sbjct: 255 --GMSPANRIDESLQVQQDGIQSAILHCKKSFHGSR--ESSLLS 275

BLAST of CmoCh16G006250 vs. ExPASy TrEMBL
Match: A0A6J1EU24 (probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN=LOC111437571 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 6.2e-231
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDED 60
           MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDED
Sbjct: 1   MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDED 60

Query: 61  DGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDGEREFNFT 120
           DGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDGEREFNFT
Sbjct: 61  DGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDGEREFNFT 120

Query: 121 LSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRV 180
           LSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRV
Sbjct: 121 LSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRV 180

Query: 181 FMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKSKLFAV 240
           FMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKSKLFAV
Sbjct: 181 FMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKSKLFAV 240

Query: 241 KLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNNNNNKPQKQSSDELTSEENRFSKDVIMQK 300
           KLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNNNNNKPQKQSSDELTSEENRFSKDVIMQK
Sbjct: 241 KLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNNNNNKPQKQSSDELTSEENRFSKDVIMQK 300

Query: 301 YLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTETETQTQTEG 360
           YLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTETETQTQTEG
Sbjct: 301 YLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTETETQTQTEG 360

Query: 361 VQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRDDSLIQQQDG 420
           VQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRDDSLIQQQDG
Sbjct: 361 VQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRDDSLIQQQDG 420

Query: 421 IQSAILHCKRSFNASRDSESSLLSSQ 447
           IQSAILHCKRSFNASRDSESSLLSSQ
Sbjct: 421 IQSAILHCKRSFNASRDSESSLLSSQ 446

BLAST of CmoCh16G006250 vs. ExPASy TrEMBL
Match: A0A6J1JD60 (probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=LOC111483413 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.5e-213
Identity = 425/453 (93.82%), Postives = 434/453 (95.81%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGAT-SAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDE 60
           MESFSLLKYWRGGGGAT  AVAAAA+ADFAFNG SSAAGAVSVVTAVPQNQAESDEENDE
Sbjct: 1   MESFSLLKYWRGGGGATPPAVAAAATADFAFNGYSSAAGAVSVVTAVPQNQAESDEENDE 60

Query: 61  DDGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEE--DDDDDEEEDGDSDGEREF 120
           DDGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEE  D+DD+EEEDGD+DGEREF
Sbjct: 61  DDGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEEDDEDDEEEEDGDTDGEREF 120

Query: 121 NFTLSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAK 180
           NFTLSSGSSNEHSDANL FSPSDDLFFKGKLVPVEASSNGGSE N RPQLRVTLLKSAAK
Sbjct: 121 NFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSETNARPQLRVTLLKSAAK 180

Query: 181 FRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKSKL 240
           FRVFMSGLK+SKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQ  KEQ+QTKSKL
Sbjct: 181 FRVFMSGLKRSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQAGKEQNQTKSKL 240

Query: 241 FAVKLKVEEVPIMSLFTRDNSSRGSGGTTS------SNNNNNNNKPQKQSSDELTSEENR 300
           FAVKLKVEEVPIMSLFTRDNSSRGSGGTTS      +NNNNNNNKPQK+SSDELTSEENR
Sbjct: 241 FAVKLKVEEVPIMSLFTRDNSSRGSGGTTSNSNNNNNNNNNNNNKPQKRSSDELTSEENR 300

Query: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTE 360
           FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSG+PPPTSPPLEKPV  
Sbjct: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPPTSPPLEKPV-- 360

Query: 361 TETQTQTEGVQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRD 420
           TETQTQTEGVQVQAPTTTANSKGR MPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRD
Sbjct: 361 TETQTQTEGVQVQAPTTTANSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRD 420

Query: 421 DSLIQQQDGIQSAILHCKRSFNASRDSESSLLS 445
           DSLIQQQDGIQSAILHCKRSFNASRDSESSLLS
Sbjct: 421 DSLIQQQDGIQSAILHCKRSFNASRDSESSLLS 451

BLAST of CmoCh16G006250 vs. ExPASy TrEMBL
Match: A0A6J1J0G8 (probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=LOC111480174 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 3.3e-176
Identity = 369/451 (81.82%), Postives = 394/451 (87.36%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDED 60
           MESFSLLKYWRGGGG      A+A+ADFAF+  SSAA A +VVTAV +NQAE+DE+ D+D
Sbjct: 1   MESFSLLKYWRGGGG--GGAPASAAADFAFSSTSSAATA-TVVTAVARNQAETDEDEDDD 60

Query: 61  DGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDGEREFNFT 120
           DGPFFDLEFAVP+EDE RKQGG+ +G RNC DGSEEE     +D+EEDGDSDGEREF+FT
Sbjct: 61  DGPFFDLEFAVPEEDEGRKQGGDGDG-RNCDDGSEEE-----NDDEEDGDSDGEREFDFT 120

Query: 121 LSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRV 180
           LSSGS+NEHSDANL FSPSDDLFFKGKLVPVEASSNGGSEPN +PQLRVTLLKSAAKFRV
Sbjct: 121 LSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAKFRV 180

Query: 181 FMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKSKLFAV 240
           FMSGLKKSKSNGEKK ETDGSVGE TK E  E T E PPEKNENQ  K Q+QTKSKLFAV
Sbjct: 181 FMSGLKKSKSNGEKKTETDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKLFAV 240

Query: 241 KLKVEEVPIMSLFTRDNSSRGSGG--TTSSNNNNNNNKPQKQSSDELTSEENRFSKDVIM 300
           KLKVEEVPIMSLFTRDNSSRGS G  TT++NNNNNNNK QKQSSDELTSEENRFSKDVIM
Sbjct: 241 KLKVEEVPIMSLFTRDNSSRGSAGTTTTTNNNNNNNNKAQKQSSDELTSEENRFSKDVIM 300

Query: 301 QKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSG-----SPPPTSPPLEKPVTETE 360
           QKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSG     + PPTSPP+EK VT+TE
Sbjct: 301 QKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGXLPSIAAPPTSPPVEKAVTDTE 360

Query: 361 TQTQTEGVQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRDDS 420
                  V+VQA TTT N+KGR MPAKLRVVCKHLGKSRSASSAVAAAPPG APSRRDDS
Sbjct: 361 L------VEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS 420

Query: 421 LIQQQDGIQSAILHCKRSFNASRDSESSLLS 445
           L+QQQDGIQSAILHCKRSFNASRDSESSLLS
Sbjct: 421 LLQQQDGIQSAILHCKRSFNASRDSESSLLS 436

BLAST of CmoCh16G006250 vs. ExPASy TrEMBL
Match: A0A6J1FPV4 (probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN=LOC111447389 PE=4 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 4.3e-176
Identity = 371/458 (81.00%), Postives = 399/458 (87.12%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDED 60
           MESFSLLKYWRGGGG   A +A+A+ADFAF+  SSAA A +VVTAV +NQAE+DE+ D+D
Sbjct: 1   MESFSLLKYWRGGGGGGGA-SASAAADFAFSSTSSAATA-TVVTAVARNQAETDEDEDDD 60

Query: 61  DGPFFDLEFAVPDEDESRKQGGNDEG---RRNCADGSEEEEEDD--DDDEEEDGDSDGER 120
           DGPFFDLEFAVPDEDE RKQGG+ +G    RNC DGSEEE +DD  +++EEEDGDSDGER
Sbjct: 61  DGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGER 120

Query: 121 EFNFTLSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSA 180
           EF+FTLSSGS+NEHSDANL FSPSDDLFFKGKLVPVEASSNGGSEPN +PQLRVTLLKSA
Sbjct: 121 EFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSA 180

Query: 181 AKFRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKS 240
           AKFRVFMSGLKKSKSNGEKK E DGSVGE TK E  E T E PPEKNENQ  K Q+QTKS
Sbjct: 181 AKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKS 240

Query: 241 KLFAVKLKVEEVPIMSLFTRDNSSRGSGG----TTSSNNNNNNNKPQKQSSDELTSEENR 300
           KLFAVKLKVEEVPIMSLFTRDNSSRGS G    TT++NNNNN+NK QKQSSDELTSEENR
Sbjct: 241 KLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENR 300

Query: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSG-----SPPPTSPPLE 360
           FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSG     + PPTSPP+E
Sbjct: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVE 360

Query: 361 KPVTETETQTQTEGVQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMA 420
           K VT+TE       V+VQA TTT N+KGR MPAKLRVVCKHLGKSRSASSAVAAAPPG A
Sbjct: 361 KTVTDTEL------VEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQA 420

Query: 421 PSRRDDSLIQQQDGIQSAILHCKRSFNASRDSESSLLS 445
           PSRRDDSL+QQQDGIQSAILHCKRSFNASRDSESSLLS
Sbjct: 421 PSRRDDSLLQQQDGIQSAILHCKRSFNASRDSESSLLS 450

BLAST of CmoCh16G006250 vs. ExPASy TrEMBL
Match: A0A5A7TMX4 (Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001450 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.4e-169
Identity = 353/446 (79.15%), Postives = 377/446 (84.53%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESD-EENDE 60
           MESFSLLKYWRGGG    A AA   ADFAFN NSSA+   +VVTAVP+ + E+D EEND 
Sbjct: 1   MESFSLLKYWRGGGAGAGATAA---ADFAFNSNSSASATATVVTAVPRKEVETDEEENDN 60

Query: 61  DDGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDG-EREFN 120
           DDGPFFDLEFAVPDEDE RKQG       +C DGSEEE E+DDDD+E+D D DG EREFN
Sbjct: 61  DDGPFFDLEFAVPDEDEGRKQG------TDCIDGSEEEGEEDDDDDEDDEDGDGDEREFN 120

Query: 121 FTLSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKF 180
           FTLSSGSSNEHSDANL FSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKF
Sbjct: 121 FTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKF 180

Query: 181 RVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKSKLF 240
           RVFMSGLKKSKSNGEKK E DGSVGE  K+E KEETT  P EKNENQ  K+Q+QTK+K F
Sbjct: 181 RVFMSGLKKSKSNGEKKTEMDGSVGETAKSETKEETTGNPSEKNENQLEKDQNQTKNKFF 240

Query: 241 AVKLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNNNNNKPQKQSSDELTSEENRFSKDVIM 300
           AVK KVEEVPIMSLFTRDNSSRGSG TT       NNKPQKQ+SDE T EE RFSKDV+M
Sbjct: 241 AVKFKVEEVPIMSLFTRDNSSRGSGVTT-------NNKPQKQTSDESTPEETRFSKDVLM 300

Query: 301 QKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTETETQTQT 360
           QKYLKMVKPLYIRVSRRYGEKF+LSGQLILGAAGAKS +PP  + P   P TE ++  +T
Sbjct: 301 QKYLKMVKPLYIRVSRRYGEKFKLSGQLILGAAGAKSSAPPSIAAPPTSPSTE-KSVVET 360

Query: 361 EGVQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMAPSRRDDSLIQQQ 420
           E VQV+ PTTT NSKGR MPAKLRVVCKHLGKSRSASSAVAAAPPG+APSRRDDSL+QQQ
Sbjct: 361 EAVQVKPPTTTTNSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGVAPSRRDDSLLQQQ 420

Query: 421 DGIQSAILHCKRSFNASRDSESSLLS 445
           DGIQSAILHCKRSFNASR SESSLLS
Sbjct: 421 DGIQSAILHCKRSFNASRGSESSLLS 429

BLAST of CmoCh16G006250 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 215.7 bits (548), Expect = 7.6e-56
Identity = 183/460 (39.78%), Postives = 247/460 (53.70%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGATSAV---AAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEEN 60
           ME+FSLL YW+  GGA + +   + ++S+         + G  ++VT+V + + E  +EN
Sbjct: 1   MEAFSLLNYWKNSGGAVTFLPHSSESSSSSLCRYSAGESIGTTTIVTSVTETEDEEADEN 60

Query: 61  DEDDGPFFDLEFAVPDEDESRKQGGNDEGRRNCADGSEEEEEDDDDDEEEDGDSDGEREF 120
           D D+GPFFDLEFA+P E+E    G   EG  +C DG                      EF
Sbjct: 61  D-DEGPFFDLEFALPAEEEDTDGGVGREGDSDCTDGG--------------------CEF 120

Query: 121 NFTLSSGSSNEHSDANLAFSPSDDLFFKGKLV-----PVEASSNGGSEPNVRPQLRVTLL 180
            FTLSS S  E     +     DD+FFK  +V     P  +     S      QL  ++L
Sbjct: 121 KFTLSSCSGGED---RVDPDSLDDVFFKETIVEEVEPPTSSEQQTCSVKAPAAQLSASIL 180

Query: 181 KSAAKFRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQ 240
           K+A K RVFM G+KKSK    K     GS       E  ++ T  PP  +    +  +SQ
Sbjct: 181 KTATKLRVFMLGMKKSKLIQAKSEANFGS-------EDLDKQTHPPPPLS----LSPESQ 240

Query: 241 TKSKLFAVKLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNNNNNKPQKQSSDELTSEENRF 300
            KS +  V LKVEEVPI+SLF+R+NSS+ S  ++SS+++++++  ++  ++ + S+E RF
Sbjct: 241 QKSTV-TVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSSSSSLKKQNGNESVVSDEKRF 300

Query: 301 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPPLEKPVTET 360
              V+MQKYLK VKPLYIRVSRRYGEK R SGQL              T+P        +
Sbjct: 301 ---VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL--------------TAP--------S 360

Query: 361 ETQTQTEGVQVQAPTTTANSKGR--TMPAKLRVVCKHLGKSRSA-------SSAVAAAPP 420
             ++  E       T T N  G    +PA L+VV KHLGKSRS+       SS  A    
Sbjct: 361 SARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTATVTT 399

Query: 421 GMAPSRRDDSLIQQQDGIQSAILHCKRSFNASRDSESSLL 444
                RRDDSL+QQQD IQSAILHCKRSFN+SRD + S+L
Sbjct: 421 QSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDKDPSVL 399

BLAST of CmoCh16G006250 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 47.8 bits (112), Expect = 2.7e-05
Identity = 77/284 (27.11%), Postives = 132/284 (46.48%), Query Frame = 0

Query: 170 TLLKSAAKFRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKE 229
           T +K   + R+  S +    + G + P+ D   GE +  E+K   ++    K   +  + 
Sbjct: 15  TTIKPRREIRISESAV--DSTTGSEDPDLDLCEGEDSFFELKISLSDF---KTPKEKQRL 74

Query: 230 QSQTKSKLFAVKLKVEEVPIMSLFTRDNSSRGSGGTTSSNNNN-----NNNKPQKQSSDE 289
           ++ T +  ++V  K + +P + + ++   S     TT   +       +  K +K ++ E
Sbjct: 75  ETTTTTTTYSVSNKSKVLPFVDISSKPQQS----PTTLLKSGQKFRAFSFKKSEKSTTTE 134

Query: 290 LTSEENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGSPPPTSPP 349
              EEN  +   +  +          R +       +    +   +   +  S       
Sbjct: 135 KKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQQTDDTMFDDSVSKRFFS------- 194

Query: 350 LEKPVTETETQTQTEGVQVQAPTT---TANSKGRTMPAKLRVVCKHLGKSRSASSAVAAA 409
           L KP+    T+ Q+    + +PT+   T   +   +P+ +R V + LGKSRSAS+A+   
Sbjct: 195 LIKPLYTKSTKKQSSST-ITSPTSSPATREKQRSNIPSGMRSVRRQLGKSRSASAAIG-- 254

Query: 410 PPGMAPSRR-DDSLIQQQDGIQSAILHCKRSFNASRDSESSLLS 445
             GM+P+ R D+SL  QQDGIQSAILHCK+SF+ SR  ESSLLS
Sbjct: 255 --GMSPANRIDESLQVQQDGIQSAILHCKKSFHGSR--ESSLLS 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SH581.1e-5439.78Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9FLX43.8e-0427.11Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ... [more]
Match NameE-valueIdentityDescription
A0A6J1EU246.2e-231100.00probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JD601.5e-21393.82probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J0G83.3e-17681.82probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FPV44.3e-17681.00probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7TMX41.4e-16979.15Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
AT1G64080.17.6e-5639.78unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G52870.12.7e-0527.11unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 347..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 252..281
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 390..412
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 330..376
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 94..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 188..223
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 116..134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..93
NoneNo IPR availablePANTHERPTHR33929:SF1MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..444
IPR039619Membrane-associated kinase regulator 2/5PANTHERPTHR33929MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G006250.1CmoCh16G006250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity