CmoCh16G005780 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G005780
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description26S proteasome non-ATPase regulatory subunit 8 homolog A-like
LocationCmo_Chr16: 2802485 .. 2806807 (-)
RNA-Seq ExpressionCmoCh16G005780
SyntenyCmoCh16G005780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAATGGCTAACGGTAAGTTTGAAGTTTGAGTTGAGTTTGTTACAAATGGCTAAGCCATGAGCGGGATGGAAATGACTAACAAATGGCTAATAGTAAATTTGAGTTGAGTTGTTACAAATGACTAACAGTAAGTTTACAAATGGCTAGTAAGTTTGAGTGCTGGGGTGGAGAGCTAGGGATGGTGCAAGACACTAGGCGCGGTAAGTTTGAGTGCTGGGGTGGAGAGCTAGGGATGGTGCAAGACACTAGGCGCGGTTGGGTTGTTACAAATGGTGGAGAGCCAGGGATGGTGCAAGACACTAGTGGAAATAGGATTAAACCACTTTTTTAATAGACACGTTTTAAAATCGTTACGGGATGGTGCAAGACACTAGTTGAAATAGGATTAAACCACTTTTTTAATAGACACGTTTTAAAACCGTTACGGGATGGTGCAAGACACTAGTTGAAATAGGATTAAACCACTTTCTTAATAGATACGTTTTAAAATTGTGAGGTTGATTGTAATTACTATACAAAAATGAACAATATCTACGGGTGATACTGACATAACTGTTCGAGCGATTTACAACTCGATGACAAGATAATTTCACACGTGTGAAAAAGTAAAAGCATGCACTACAGTTTAACTTAATTTAAATAACTTAGCCTAAATTTTTTTTTAAGTATGATTAGTAATAAATTTCCTCTCCTAAGTCGGGTTCTTTTATAAGTTTATTACAGTATATGGGCCGGGTATGGGTATCGGTTCGGATTTGACCCATTTGAATCCTTCTTGAAGAGCAAGCAAAGACAACAAACAAAGGTGACCATCTTCATTCCAATTTCAGCAGTGATTCGGAGGAAGAAATTGAAGGAGACGAAGATCGCCATGGAACCAAAGCTAACTGAGGACTCGCAGCACTTTGATCGCTTCAAAGCTGCGTTTGTTAGGCACGATTACGATTCTTGTAGCAATCTCCTCCCTCAGCTAAAGGTGTCGATTTTATTTCAGAACCCTAATGTAACCCTATGACTCCTCCGTTCGATACGTCACTGGAGTTTTAGCGTGTTTTCTTCTTTTCTCGAGGAGTCGTTTTGATAATGTTGTTCAAGTTTCCGAAATCTTTCTTCATTTTATTTTTGCAGGTTTTTTTAACTGGATTCAGAAGTCTTCCACCATTGTTTGAAGATACGCCCAATGCAGTCCATGAATTGACGGTAGCTAGTAAGAATTCATACTCCATGCCGTAGTTCTATAGCTCTTATTGGCTCGCCTATATTTTTCTGTACGAATAATTTGGAATAGTTTGCTGTCGTTTGGGTCTTGAAATTACTCAACTGAAGAGAAACTTATAGTCTGGCCATGCATCTTCATAGTAACATATGGAGACTTATATTTGTGTGGAGCTTGGTAGTTGTTATTCAATTGCATGATTAGTGACTGTAAAGCTACAACCCGAACTGCAAGCATAAGCTACTAGATTCTGTAATCTACAATGATAAGATTATTATGGAGCCTTGGAACCATTTTCCCATCTTAGAAGCTAGATGACCGATACCAGTCGATGAAAATTTGTCTAACGTTTTAATGAGCCATTGAAGCAATTTTACTCAGATGTACGAAATATGAGAAACAAATGTGGTAAATTGATTAACTATCTAATAAAAATTTGAGAATCACAGAAACTTGATCACTTGGACGTGGATAAATAATTTACTTTCTGGGTCATTGAATATTCTGATGCAGAATTTTCTGTACGGAAGAGTTTGTTATCTGTATGGAACTTTGGTTTTTTCTTTCCCTGTATAGTCATATTCTAAATTTAAATCTTGTACAATTTTTATGTTCACTTGGGGACTTCAGGGGATATATACGAGCATGCTGTATTACTTAGTGTGAAGATTGAAGATCAAGATGCATTTGAAAGAGATTTCTTCCAATTGAAGCCTTACTATACTGATGCTCGGTATGTTTCTCTTAAGTGAAATTTGAATTCCTAAATTCTATCTAATTGATTAAATTCTCGGTATATTTGCTTGTTTATTCAGACTTGGGGGGGGGGGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGGCGTAAGAGTTAAATGATTTATACTCATTCACGTAGATATAAATTTAACTGAAGTTCTATTGAGCTATTTGATTCTTTGTTTATTTAAAGTGAAACATCATCGAAAACAAAAGCTGCTTATTGATATTATTTGCAATTATAATTCTTAGTTCGTCAGTATTCACTAGTTTACTTTAATGTATTATCTTCTTTCTTTCATGTAAATAAGTTAATTAATTCAACTGAACTGATTCAGTGAGATGGTGGACTGACAATGAATTGCTGCAGATTAGCAAATGGATCCAAGCTTATCAGACAAACGTCTTCTATGAGTTATATTTATGTTCAAAACTGATATCTAATCTATAACTTTAGGCTGAGCTTCCTGTAGTTATCTTGCATTTTACCAGAATTGCCACATTGAGATCACTTGATATTACCTTGTGAGTTTGAACTAATTTTTTCTCATGAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGACCAGTAACCGCCTTCCGCCATNCCCTCAGGAGTATCCCATCTTGGGTCTCAACCTTTTGAGGCTTCTTGTACAGAACAGAATTGCTGAATTTCATACTGAACTTGAACTTCTCTCCACCTCTGCTCTGGAGAACCCATGCATTAAACATGCTGTAGAGCTGGAGCAATCTTTCATGGAAGGGGCTTACAACCGTGTATTGAGTGCTCGACAGACAGTGCCACATGAAACTTTTGGTTATTTTATGGACCTCCTTGCAAAGACTGTAAGGTAACTCATCTAAATAAGGTGTCTAAGTAATATAGTGATAGATGAGAAATCCCACCTCCTTCGAAGTGTTTGCCGAATTCTCTCTTACTCTCTCTTCTTACTTGGGGTCACTGACAAGTAGCAGTTCCATTTCATTCTTAGTTTCTATTCATAGTTTGCGTCTTTTTTGCCACTTCCAGGGATGAAATAGCCGGTTGCAGTGAAAAGGCATATGATTATCTTTCAATAAGTAATGCCCGCCAAATGCTGCTGTTTTCCTCTGACCAGGAACTTTTGGAATATGTCAAGGAGGTAACCTCTGTTAAGTCTGTTGGATGGTATCTAGACATCGATAATCTACGTAATCACTAAAATGTTTTAGGTTAGACCAAAGTTTTCTTTTTTCACTGAACTGCGAAACATTTACCATGATGCGGTGCATATAATCCATTGAACCGATCTGCAGTATGTTGAGTGCCACCCTGGCTATCTAAGGGGATGATCATGAGTTTATAAGTAAGGAATACATTTTCATTGGTATGAGGTCTTTTGGGAAAACGAAAACTAAAACCATTAGAGCTTATGCTCAAAGTGGACAATATCATGTCAGATTATGAAGGGTCCGTGGTTCCTAACACAGTATGCAGGTTAGGAGTAGTTTCCATTGGAGGGTTTGCACCTGTTGATGGAAATCTTATGTTTCAATGCAGGAGCATCCCGAGTGGGAGATAACGAACGGCATCTTATATTTCCAGAAGGTGAAAGAATCTGCACCTTGCAAGGAAATTCCTTCTCTTCAACTTATCAACCAAACACTCAGTTACGCAAGGGAGTTGGAGCGGATTGTATGAACCAAGCTAGCAGCTTCTCAGAATTGTCATCTGCTGATTTACAACATGCCAAGGTTTTATGATTCTCCCTCCCCTCTCTTATGATCATTATGGGGTTTTTTTTTTCTTTTTCCAAACAAAGTAATTTGTTCGTTGACATCGACTCAGATTTGCATCAAATTATCTTGGTATCTTCATTAACATAAGATGACTGTTCATGCATTTTTCTTTTCCAAGTGTTTGTTTACAATTACATATCCTCGGCATCTAGTTAGATTCCAACGTCAAGGAAGTCGTTATCGTTTGATTATTCCTATGGTATTAAAACATGGCTTCAAACATTACTTTGAAGCTCAAGTTTACAAAATGGGTTTCCATGCATTCGAATCTAATAAAATTGTGATTCAAATACTAAGGTGTTTGGAATTGCAGAGCAGAGTTGCTTCAGGTTCGGAAATTTTTGAAGCGATGAAAAAATGAAATAATTAGAGGAGAAAGGTACGTTATAATATTTTAAACTTTTTAGCCATGTTCCATTGACCTGGG

mRNA sequence

ATGGAAATGGCTAACGTGATTCGGAGGAAGAAATTGAAGGAGACGAAGATCGCCATGGAACCAAAGCTAACTGAGGACTCGCAGCACTTTGATCGCTTCAAAGCTGCGTTTGTTAGGCACGATTACGATTCTTGTAGCAATCTCCTCCCTCAGCTAAAGGTTTTTTTAACTGGATTCAGAAGTCTTCCACCATTGTTTGAAGATACGCCCAATGCAGTCCATGAATTGACGGTAGCTAGGGATATATACGAGCATGCTGTATTACTTAGTGTGAAGATTGAAGATCAAGATGCATTTGAAAGAGATTTCTTCCAATTGAAGCCTTACTATACTGATGCTCGTAACCGCCTTCCGCCATNCCCTCAGGAGTATCCCATCTTGGGTCTCAACCTTTTGAGGCTTCTTGTACAGAACAGAATTGCTGAATTTCATACTGAACTTGAACTTCTCTCCACCTCTGCTCTGGAGAACCCATGCATTAAACATGCTGTAGAGCTGGAGCAATCTTTCATGGAAGGGGCTTACAACCGTGTATTGAGTGCTCGACAGACAGTGCCACATGAAACTTTTGGTTATTTTATGGACCTCCTTGCAAAGACTGTAAGGGATGAAATAGCCGGTTGCAGTGAAAAGGCATATGATTATCTTTCAATAAGTAATGCCCGCCAAATGCTGCTGTTTTCCTCTGACCAGGAACTTTTGGAATATGTCAAGGAGGAGCATCCCGAGTGGGAGATAACGAACGGCATCTTATATTTCCAGAAGGTGAAAGAATCTGCACCTTGCAAGGAAATTCCTTCTCTTCAACTTATCAACCAAACACTCAGTTACGCAAGGGAGTTGGAGCGGATTGTATGAACCAAGCTAGCAGCTTCTCAGAATTGTCATCTGCTGATTTACAACATGCCAAGAGCAGAGTTGCTTCAGGTTCGGAAATTTTTGAAGCGATGAAAAAATGAAATAATTAGAGGAGAAAGGTACGTTATAATATTTTAAACTTTTTAGCCATGTTCCATTGACCTGGG

Coding sequence (CDS)

ATGGAAATGGCTAACGTGATTCGGAGGAAGAAATTGAAGGAGACGAAGATCGCCATGGAACCAAAGCTAACTGAGGACTCGCAGCACTTTGATCGCTTCAAAGCTGCGTTTGTTAGGCACGATTACGATTCTTGTAGCAATCTCCTCCCTCAGCTAAAGGTTTTTTTAACTGGATTCAGAAGTCTTCCACCATTGTTTGAAGATACGCCCAATGCAGTCCATGAATTGACGGTAGCTAGGGATATATACGAGCATGCTGTATTACTTAGTGTGAAGATTGAAGATCAAGATGCATTTGAAAGAGATTTCTTCCAATTGAAGCCTTACTATACTGATGCTCGTAACCGCCTTCCGCCATNCCCTCAGGAGTATCCCATCTTGGGTCTCAACCTTTTGAGGCTTCTTGTACAGAACAGAATTGCTGAATTTCATACTGAACTTGAACTTCTCTCCACCTCTGCTCTGGAGAACCCATGCATTAAACATGCTGTAGAGCTGGAGCAATCTTTCATGGAAGGGGCTTACAACCGTGTATTGAGTGCTCGACAGACAGTGCCACATGAAACTTTTGGTTATTTTATGGACCTCCTTGCAAAGACTGTAAGGGATGAAATAGCCGGTTGCAGTGAAAAGGCATATGATTATCTTTCAATAAGTAATGCCCGCCAAATGCTGCTGTTTTCCTCTGACCAGGAACTTTTGGAATATGTCAAGGAGGAGCATCCCGAGTGGGAGATAACGAACGGCATCTTATATTTCCAGAAGGTGAAAGAATCTGCACCTTGCAAGGAAATTCCTTCTCTTCAACTTATCAACCAAACACTCAGTTACGCAAGGGAGTTGGAGCGGATTGTATGA

Protein sequence

MEMANVIRRKKLKETKIAMEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTVARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQNRIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLAKTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKESAPCKEIPSLQLINQTLSYARELERIV
Homology
BLAST of CmoCh16G005780 vs. ExPASy Swiss-Prot
Match: Q9SGW3 (26S proteasome non-ATPase regulatory subunit 8 homolog A OS=Arabidopsis thaliana OX=3702 GN=RPN12A PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 6.5e-122
Identity = 217/267 (81.27%), Postives = 239/267 (89.51%), Query Frame = 0

Query: 19  MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 78
           M+P+LTE SQ F+RFKAAF R DY++CS+LL QLKV LT F SLPPLFE++PNA  ELT+
Sbjct: 1   MDPQLTEVSQQFERFKAAFARKDYNTCSDLLSQLKVLLTKFTSLPPLFENSPNAAKELTI 60

Query: 79  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 138
           ARDIYEHAV+LSVK EDQDAFERDFFQLKPYY DARNR+P  PQE  ILGLNLLRLLVQN
Sbjct: 61  ARDIYEHAVVLSVKTEDQDAFERDFFQLKPYYVDARNRIPQSPQENLILGLNLLRLLVQN 120

Query: 139 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 198
           RIAEFHTELELLS++ LE+PCIKHAVELEQSFMEGAYNRVLSARQT P  T+ YFMDLLA
Sbjct: 121 RIAEFHTELELLSSATLEDPCIKHAVELEQSFMEGAYNRVLSARQTAPDATYVYFMDLLA 180

Query: 199 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 258
           KT+RDEIAGCSEKAYDY+SIS+ARQMLLFSSDQELL YV +EHPEWE+  G + FQK KE
Sbjct: 181 KTIRDEIAGCSEKAYDYVSISDARQMLLFSSDQELLTYVTDEHPEWEVKEGFVVFQKAKE 240

Query: 259 SAPCKEIPSLQLINQTLSYARELERIV 286
           +APCKEIPSLQLINQTLSYARELERIV
Sbjct: 241 TAPCKEIPSLQLINQTLSYARELERIV 267

BLAST of CmoCh16G005780 vs. ExPASy Swiss-Prot
Match: Q9FHY0 (Putative 26S proteasome non-ATPase regulatory subunit 8 homolog B OS=Arabidopsis thaliana OX=3702 GN=RPN12B PE=5 SV=2)

HSP 1 Score: 315.1 bits (806), Expect = 8.2e-85
Identity = 169/267 (63.30%), Postives = 197/267 (73.78%), Query Frame = 0

Query: 19  MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 78
           M+P+L E SQ F+RFKAAF+  D+D+CS+LL QLK+F     SL      + NA+  LT 
Sbjct: 1   MDPQLMEVSQQFERFKAAFIIKDFDTCSSLLSQLKLFDHYLISL------SLNALLLLTC 60

Query: 79  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 138
           A                             ++   RNR+PP PQE  I+GLNLLRLLVQN
Sbjct: 61  AL----------------------------FFLCTRNRIPPSPQENLIMGLNLLRLLVQN 120

Query: 139 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 198
           RIAEFHTEL LLS++ LENPCIKHAVELEQSFMEGAYNRVLSARQT P ET+ YFMDLLA
Sbjct: 121 RIAEFHTELGLLSSATLENPCIKHAVELEQSFMEGAYNRVLSARQTAPDETYVYFMDLLA 180

Query: 199 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 258
           KT+RDEIAGCSEKAYD+LSIS   +MLLFSSDQ+LL YV EEHPEWE+ +G++ FQK +E
Sbjct: 181 KTIRDEIAGCSEKAYDHLSISEGCKMLLFSSDQQLLTYVNEEHPEWEVKDGLVVFQKTRE 233

Query: 259 SAPCKEIPSLQLINQTLSYARELERIV 286
           +APCKEIPSLQLINQTLSY RELERI+
Sbjct: 241 TAPCKEIPSLQLINQTLSYTRELERIL 233

BLAST of CmoCh16G005780 vs. ExPASy Swiss-Prot
Match: P02889 (Probable 26S proteasome non-ATPase regulatory subunit 8 OS=Dictyostelium discoideum OX=44689 GN=psmD8-1 PE=2 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 103/260 (39.62%), Postives = 164/260 (63.08%), Query Frame = 0

Query: 28  QHFDRFKAAFV-RHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAV-HELTVARDIYEH 87
           Q+ + FK       D    + +L QLK+  T     P    +  + V  +L +AR+I E 
Sbjct: 8   QNLNNFKKLVAGNSDKAQITPVLVQLKLAATIHLEKPTSLSNVSDKVKKDLVLAREILEL 67

Query: 88  AVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQNRIAEFHT 147
             L S+KI+D D+FER F QLK YY D ++ + P   EY I+GLNL+RLL +++ +EFH+
Sbjct: 68  ISLYSIKIKDIDSFERTFNQLKTYYYDYKSIIAPSTLEYQIIGLNLMRLLAKHKTSEFHS 127

Query: 148 ELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLAKTVRDEI 207
           E+EL+  + L+N  IK  + +E+S  EG+YN+++ +R  VP E +  F+D+LA +++++I
Sbjct: 128 EIELIEFNNLDNSFIKFPLLVEKSITEGSYNKIIQSRSGVPSEYYQVFLDILADSIKEDI 187

Query: 208 AGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKESAPCKEI 267
           A CSEK++  LS+ +A ++LLF+ + +  +Y+KE +  W++   ++ F          EI
Sbjct: 188 ANCSEKSFKTLSLKDAEKVLLFNDNNQFQQYIKERN--WKVQGDVIQFG--NNDNQTVEI 247

Query: 268 PSLQLINQTLSYARELERIV 286
           PSLQLI+QTL YA+ELERIV
Sbjct: 248 PSLQLIHQTLHYAKELERIV 263

BLAST of CmoCh16G005780 vs. ExPASy Swiss-Prot
Match: Q9CX56 (26S proteasome non-ATPase regulatory subunit 8 OS=Mus musculus OX=10090 GN=Psmd8 PE=1 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 1.9e-41
Identity = 100/243 (41.15%), Postives = 141/243 (58.02%), Query Frame = 0

Query: 45  CSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTVARDIYEHAVLLSVKIEDQDAFERDFF 104
           C   L +LK+ L     LP     T     +L +ARDI E     S+  +D  +FER   
Sbjct: 115 CGEELGRLKLVLLELNFLPT--TGTKLTKQQLILARDILEIGAQWSILCKDIPSFERYMA 174

Query: 105 QLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQNRIAEFHTELELLSTSALE-NPCIKHA 164
           QLK YY D + +LP     + +LGLNLL LL QNR+AEFHTELE L    ++ N  IKH 
Sbjct: 175 QLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQTNVYIKHP 234

Query: 165 VELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLAKTVRDEIAGCSEKAYDYLSISNARQ 224
           V LEQ  MEG+YN+V  A+  +P E++ +F+D+L  T+RDEIAGC EKAY+ +  + A +
Sbjct: 235 VSLEQYLMEGSYNKVFLAKGNIPAESYTFFIDILLDTIRDEIAGCIEKAYEKILFAEATR 294

Query: 225 MLLFSSDQELLEYVKEEHPEWEI-TNGILYFQKVKESAPCKEIPSLQLINQTLSYARELE 284
           +L FS+ +++ +Y K+    W +  N    F   ++      IPS +L  Q + YAR+LE
Sbjct: 295 ILFFSTPKKMTDYAKKR--GWVLGPNNYYSFASQQQKPEDSTIPSTELAKQVIEYARQLE 353

Query: 285 RIV 286
            IV
Sbjct: 355 MIV 353

BLAST of CmoCh16G005780 vs. ExPASy Swiss-Prot
Match: Q5RE15 (26S proteasome non-ATPase regulatory subunit 8 (Fragment) OS=Pongo abelii OX=9601 GN=PSMD8 PE=2 SV=2)

HSP 1 Score: 169.5 bits (428), Expect = 5.6e-41
Identity = 99/243 (40.74%), Postives = 141/243 (58.02%), Query Frame = 0

Query: 45  CSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTVARDIYEHAVLLSVKIEDQDAFERDFF 104
           C   L +LK+ L     LP     T     +L +ARDI E     S+  +D  +FER   
Sbjct: 51  CGEELGRLKLVLLELNFLPT--TGTKLTKQQLILARDILEIGAQWSILRKDIPSFERYMA 110

Query: 105 QLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQNRIAEFHTELELLSTSALE-NPCIKHA 164
           QLK YY D + +LP     + +LGLNLL LL QNR+AEFHTELE L    ++ N  IKH 
Sbjct: 111 QLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQTNVYIKHP 170

Query: 165 VELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLAKTVRDEIAGCSEKAYDYLSISNARQ 224
           V LEQ  MEG+YN+V  A+  +P E++ +F+D+L  T+RDEIAGC EKAY+ +  + A +
Sbjct: 171 VSLEQYLMEGSYNKVFLAKGNIPAESYAFFIDILLDTIRDEIAGCIEKAYEKILFTEATR 230

Query: 225 MLLFSSDQELLEYVKEEHPEWEI-TNGILYFQKVKESAPCKEIPSLQLINQTLSYARELE 284
           +L F++ +++ +Y K+    W +  N    F   ++      IPS +L  Q + YAR+LE
Sbjct: 231 ILFFNTPKKMTDYAKKR--GWVLGPNNYYSFASQQQKPEDTTIPSTELAKQVIEYARQLE 289

Query: 285 RIV 286
            IV
Sbjct: 291 MIV 289

BLAST of CmoCh16G005780 vs. ExPASy TrEMBL
Match: A0A6J1EZQ3 (26S proteasome non-ATPase regulatory subunit 8 homolog A-like OS=Cucurbita moschata OX=3662 GN=LOC111437694 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 19  MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 78
           MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV
Sbjct: 1   MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 60

Query: 79  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 138
           ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN
Sbjct: 61  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 120

Query: 139 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 198
           RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA
Sbjct: 121 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 180

Query: 199 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 258
           KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE
Sbjct: 181 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 240

Query: 259 SAPCKEIPSLQLINQTLSYARELERIV 286
           SAPCKEIPSLQLINQTLSYARELERIV
Sbjct: 241 SAPCKEIPSLQLINQTLSYARELERIV 267

BLAST of CmoCh16G005780 vs. ExPASy TrEMBL
Match: A0A6J1J7S1 (26S proteasome non-ATPase regulatory subunit 8 homolog A-like OS=Cucurbita maxima OX=3661 GN=LOC111483349 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.3e-145
Identity = 261/267 (97.75%), Postives = 263/267 (98.50%), Query Frame = 0

Query: 19  MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 78
           MEPKLTE SQH DRFKAAFVRHDYDSCSNLL QLKVFLTGFRSLPPLFEDTPNAVHELTV
Sbjct: 1   MEPKLTEVSQHIDRFKAAFVRHDYDSCSNLLSQLKVFLTGFRSLPPLFEDTPNAVHELTV 60

Query: 79  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 138
           ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPP PQEYPILGLNLLRLLVQN
Sbjct: 61  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPSPQEYPILGLNLLRLLVQN 120

Query: 139 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 198
           RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA
Sbjct: 121 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 180

Query: 199 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 258
           KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVK+EHPEWEITNGILYFQKVKE
Sbjct: 181 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKKEHPEWEITNGILYFQKVKE 240

Query: 259 SAPCKEIPSLQLINQTLSYARELERIV 286
           SAPCKEIPS+QLINQTLSYARELERIV
Sbjct: 241 SAPCKEIPSVQLINQTLSYARELERIV 267

BLAST of CmoCh16G005780 vs. ExPASy TrEMBL
Match: A0A5D3D1F4 (26S proteasome non-ATPase regulatory subunit 8-like protein A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003260 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.6e-145
Identity = 259/279 (92.83%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 7   IRRKKLKETKIAMEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLF 66
           I+R+KLK+ KIAM+PKLTE SQHF+RFKAA VRHDYDSC+NLL QLKVFLTGFRSLPPLF
Sbjct: 39  IQRRKLKK-KIAMDPKLTEVSQHFERFKAALVRHDYDSCNNLLSQLKVFLTGFRSLPPLF 98

Query: 67  EDTPNAVHELTVARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPI 126
           EDTPNAVHELT+ARDIYEHAVLLSVK+EDQDAFERDFFQLKPYYTDARNRLPP PQEYPI
Sbjct: 99  EDTPNAVHELTIARDIYEHAVLLSVKVEDQDAFERDFFQLKPYYTDARNRLPPSPQEYPI 158

Query: 127 LGLNLLRLLVQNRIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVP 186
           LGLNLLRLLVQNRIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVP
Sbjct: 159 LGLNLLRLLVQNRIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVP 218

Query: 187 HETFGYFMDLLAKTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEI 246
           HET+GYFMDLLAKTVRDEIAGCSEKAYDYLSI++ARQMLLFSSDQELL+YVKEEHPEWEI
Sbjct: 219 HETYGYFMDLLAKTVRDEIAGCSEKAYDYLSINDARQMLLFSSDQELLDYVKEEHPEWEI 278

Query: 247 TNGILYFQKVKESAPCKEIPSLQLINQTLSYARELERIV 286
           TNG++YFQK KESAPCKEIPSLQLINQTLSYARELERIV
Sbjct: 279 TNGVVYFQKAKESAPCKEIPSLQLINQTLSYARELERIV 316

BLAST of CmoCh16G005780 vs. ExPASy TrEMBL
Match: A0A5A7TS32 (26S proteasome non-ATPase regulatory subunit 8-like protein A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002620 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.7e-144
Identity = 257/279 (92.11%), Postives = 272/279 (97.49%), Query Frame = 0

Query: 7   IRRKKLKETKIAMEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLF 66
           I+R+KLK+ KI M+PKLTE SQHF+RFKAA VRHDYDSC+NLL QLKV+LTGFRSLPPLF
Sbjct: 39  IQRRKLKK-KIVMDPKLTEVSQHFERFKAALVRHDYDSCNNLLSQLKVYLTGFRSLPPLF 98

Query: 67  EDTPNAVHELTVARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPI 126
           EDTPNAVHELT+ARDIYEHAVLLSVK+EDQDAFERDFFQLKPYYTDARNRLPP PQEYPI
Sbjct: 99  EDTPNAVHELTIARDIYEHAVLLSVKVEDQDAFERDFFQLKPYYTDARNRLPPSPQEYPI 158

Query: 127 LGLNLLRLLVQNRIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVP 186
           LGLNLLRLLVQNRIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVP
Sbjct: 159 LGLNLLRLLVQNRIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVP 218

Query: 187 HETFGYFMDLLAKTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEI 246
           HET+GYFMDLLAKTVRDEIAGCSEKAYDYLSI++ARQMLLFSSDQELL+YVKEEHPEWEI
Sbjct: 219 HETYGYFMDLLAKTVRDEIAGCSEKAYDYLSINDARQMLLFSSDQELLDYVKEEHPEWEI 278

Query: 247 TNGILYFQKVKESAPCKEIPSLQLINQTLSYARELERIV 286
           TNG++YFQK KESAPCKEIPSLQLINQTLSYARELERIV
Sbjct: 279 TNGVVYFQKAKESAPCKEIPSLQLINQTLSYARELERIV 316

BLAST of CmoCh16G005780 vs. ExPASy TrEMBL
Match: A0A1S3BSK8 (26S proteasome non-ATPase regulatory subunit 8 homolog A OS=Cucumis melo OX=3656 GN=LOC103493070 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.5e-142
Identity = 251/267 (94.01%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 19  MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 78
           M+PKLTE SQHF+RFKAA VRHDYDSC+NLL QLKVFLTGFRSLPPLFEDTPNAVHELT+
Sbjct: 1   MDPKLTEVSQHFERFKAALVRHDYDSCNNLLSQLKVFLTGFRSLPPLFEDTPNAVHELTI 60

Query: 79  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 138
           ARDIYEHAVLLSVK+EDQDAFERDFFQLKPYYTDARNRLPP PQEYPILGLNLLRLLVQN
Sbjct: 61  ARDIYEHAVLLSVKVEDQDAFERDFFQLKPYYTDARNRLPPSPQEYPILGLNLLRLLVQN 120

Query: 139 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 198
           RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHET+GYFMDLLA
Sbjct: 121 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETYGYFMDLLA 180

Query: 199 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 258
           KTVRDEIAGCSEKAYDYLSI++ARQMLLFSSDQELL+YVKEEHPEWEITNG++YFQK KE
Sbjct: 181 KTVRDEIAGCSEKAYDYLSINDARQMLLFSSDQELLDYVKEEHPEWEITNGVVYFQKAKE 240

Query: 259 SAPCKEIPSLQLINQTLSYARELERIV 286
           SAPCKEIPSLQLINQTLSYARELERIV
Sbjct: 241 SAPCKEIPSLQLINQTLSYARELERIV 267

BLAST of CmoCh16G005780 vs. TAIR 10
Match: AT1G64520.1 (regulatory particle non-ATPase 12A )

HSP 1 Score: 438.3 bits (1126), Expect = 4.6e-123
Identity = 217/267 (81.27%), Postives = 239/267 (89.51%), Query Frame = 0

Query: 19  MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 78
           M+P+LTE SQ F+RFKAAF R DY++CS+LL QLKV LT F SLPPLFE++PNA  ELT+
Sbjct: 1   MDPQLTEVSQQFERFKAAFARKDYNTCSDLLSQLKVLLTKFTSLPPLFENSPNAAKELTI 60

Query: 79  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 138
           ARDIYEHAV+LSVK EDQDAFERDFFQLKPYY DARNR+P  PQE  ILGLNLLRLLVQN
Sbjct: 61  ARDIYEHAVVLSVKTEDQDAFERDFFQLKPYYVDARNRIPQSPQENLILGLNLLRLLVQN 120

Query: 139 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 198
           RIAEFHTELELLS++ LE+PCIKHAVELEQSFMEGAYNRVLSARQT P  T+ YFMDLLA
Sbjct: 121 RIAEFHTELELLSSATLEDPCIKHAVELEQSFMEGAYNRVLSARQTAPDATYVYFMDLLA 180

Query: 199 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 258
           KT+RDEIAGCSEKAYDY+SIS+ARQMLLFSSDQELL YV +EHPEWE+  G + FQK KE
Sbjct: 181 KTIRDEIAGCSEKAYDYVSISDARQMLLFSSDQELLTYVTDEHPEWEVKEGFVVFQKAKE 240

Query: 259 SAPCKEIPSLQLINQTLSYARELERIV 286
           +APCKEIPSLQLINQTLSYARELERIV
Sbjct: 241 TAPCKEIPSLQLINQTLSYARELERIV 267

BLAST of CmoCh16G005780 vs. TAIR 10
Match: AT5G42040.1 (regulatory particle non-ATPase 12B )

HSP 1 Score: 315.1 bits (806), Expect = 5.9e-86
Identity = 169/267 (63.30%), Postives = 197/267 (73.78%), Query Frame = 0

Query: 19  MEPKLTEDSQHFDRFKAAFVRHDYDSCSNLLPQLKVFLTGFRSLPPLFEDTPNAVHELTV 78
           M+P+L E SQ F+RFKAAF+  D+D+CS+LL QLK+F     SL      + NA+  LT 
Sbjct: 1   MDPQLMEVSQQFERFKAAFIIKDFDTCSSLLSQLKLFDHYLISL------SLNALLLLTC 60

Query: 79  ARDIYEHAVLLSVKIEDQDAFERDFFQLKPYYTDARNRLPPXPQEYPILGLNLLRLLVQN 138
           A                             ++   RNR+PP PQE  I+GLNLLRLLVQN
Sbjct: 61  AL----------------------------FFLCTRNRIPPSPQENLIMGLNLLRLLVQN 120

Query: 139 RIAEFHTELELLSTSALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETFGYFMDLLA 198
           RIAEFHTEL LLS++ LENPCIKHAVELEQSFMEGAYNRVLSARQT P ET+ YFMDLLA
Sbjct: 121 RIAEFHTELGLLSSATLENPCIKHAVELEQSFMEGAYNRVLSARQTAPDETYVYFMDLLA 180

Query: 199 KTVRDEIAGCSEKAYDYLSISNARQMLLFSSDQELLEYVKEEHPEWEITNGILYFQKVKE 258
           KT+RDEIAGCSEKAYD+LSIS   +MLLFSSDQ+LL YV EEHPEWE+ +G++ FQK +E
Sbjct: 181 KTIRDEIAGCSEKAYDHLSISEGCKMLLFSSDQQLLTYVNEEHPEWEVKDGLVVFQKTRE 233

Query: 259 SAPCKEIPSLQLINQTLSYARELERIV 286
           +APCKEIPSLQLINQTLSY RELERI+
Sbjct: 241 TAPCKEIPSLQLINQTLSYTRELERIL 233

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SGW36.5e-12281.2726S proteasome non-ATPase regulatory subunit 8 homolog A OS=Arabidopsis thaliana... [more]
Q9FHY08.2e-8563.30Putative 26S proteasome non-ATPase regulatory subunit 8 homolog B OS=Arabidopsis... [more]
P028891.2e-4639.62Probable 26S proteasome non-ATPase regulatory subunit 8 OS=Dictyostelium discoid... [more]
Q9CX561.9e-4141.1526S proteasome non-ATPase regulatory subunit 8 OS=Mus musculus OX=10090 GN=Psmd8... [more]
Q5RE155.6e-4140.7426S proteasome non-ATPase regulatory subunit 8 (Fragment) OS=Pongo abelii OX=960... [more]
Match NameE-valueIdentityDescription
A0A6J1EZQ31.1e-148100.0026S proteasome non-ATPase regulatory subunit 8 homolog A-like OS=Cucurbita mosch... [more]
A0A6J1J7S13.3e-14597.7526S proteasome non-ATPase regulatory subunit 8 homolog A-like OS=Cucurbita maxim... [more]
A0A5D3D1F45.6e-14592.8326S proteasome non-ATPase regulatory subunit 8-like protein A OS=Cucumis melo va... [more]
A0A5A7TS323.7e-14492.1126S proteasome non-ATPase regulatory subunit 8-like protein A OS=Cucumis melo va... [more]
A0A1S3BSK84.5e-14294.0126S proteasome non-ATPase regulatory subunit 8 homolog A OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT1G64520.14.6e-12381.27regulatory particle non-ATPase 12A [more]
AT5G42040.15.9e-8663.30regulatory particle non-ATPase 12B [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033464CSN8/PSMD8/EIF3KPFAMPF10075CSN8_PSD8_EIF3Kcoord: 123..257
e-value: 4.3E-25
score: 88.4
NoneNo IPR availableGENE3D1.25.40.990coord: 22..255
e-value: 3.4E-82
score: 277.2
NoneNo IPR availablePANTHERPTHR12387:SF5BNACNNG39010D PROTEINcoord: 19..284
IPR00674626S proteasome non-ATPase regulatory subunit Rpn12PANTHERPTHR1238726S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8coord: 19..284
IPR000717Proteasome component (PCI) domainPROSITEPS50250PCIcoord: 97..268
score: 18.573544

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G005780.1CmoCh16G005780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005838 proteasome regulatory particle