CmoCh16G005650 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G005650
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
LocationCmo_Chr16: 2751710 .. 2755013 (+)
RNA-Seq ExpressionCmoCh16G005650
SyntenyCmoCh16G005650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTAGTTCCAGAGGCAACTGCCGGGAGGAGGAGGAGGAGGAGGAGGACGGAAGAGGAATCCGCCTCCGCCTCAGCCGCCGCCATCGTCGAGATCGAGGTCGATTTGTGATGGCCGGCCAGTCGCGGAAGTGGCTGGTTCTGGTGGCGACGATATGGATTCAAGCATTCACCGGCACGAACTTCGATTTCTCGGCCTATTCTTCGAGATTGAAATCGGTATTAGGCATTTCTCAGGTTCAACTTAATTACCTCGCCACCGCCTCCGATCTAGGCAAGGTTTTCGGCTGGTCCTCTGGATTAGCTCTGCTTCACCTACCGTTGCCGGTGGTTCTGTTCATCGCCGCTTTCTTAGGACTAATCGGTTACGGCTTCCAATGGCTCCTAATCGCCGATTTCATCTCCTTGCCCTATTTCCTGGTACTCTCTCTCTCTCTCTCTCTCTAGAAATCTTTGTTTCTTTCAACTGCTTCTCTGTTTCCTTATCGATACTTCAAAATATTGGATGAAATTTCTGTTCGTTTCTCCCAAAACAAAACCGGATTCCGTAAATCGATTGGGGATGGATTATGAATATTATTATTTTTTTAAGTTCAGTAGAGAAATGAACTATGAACTATGAACATGGTTCCTGATTCGTTTCTCCCAAGATTTTCTTGGGATTATAAAAACTGCCGTCGTTCTTGAAAAATTCTACAACTTATTCGGATTGTTTTCCTGTTAGAAAATTATTTAGTAGTTTAGTATTTTTAAATCGTTTCTGTTTTTTCAAATCATGTTTTCTATGGAATTGAATCTAAAAAAAAAAAGAAAAAAAAGAAATATATAAACAAAAAACAGCGAGATCGAGAATTATTTATATATTTGGAAGCGAACAGAATATTATTATTTCTCACCTAATTTTCGAATTAATACATTATATTTAAATATGTTGACAAAAATATAATACGTTAAAAATAATAAAACCAAGATTTTTCATTAAAGAGACAAAAATATAACATTATCAATGCAATTTTTTTGGAAATATTTAAAAGTATTTTGAGAACCTCATTTTTTTTTTCTTTCTAATTTATGAGAAAATTTAATTTGGTTGCAGTATTTTTATGGTATTTTGAAAGACATCAACACATGACGCCCATGTGGATCTCTGTTTTCTGTGTAAAAAATCCAAAACAATATTATTATAATTTTAATCACATCTTTTATTCATCAAATAATTTTTATTTTAATATAAGTAATAATTTTCAATTCAAACTATTACAAAGTGGGGCACTTTATTTTTTTTATTTCTACATCTAACTCAATGAGGTTGTTGTCTTTTAAAAAATAAGTAAGGTATACATATGATAAAATAATTACCACATTTTTAAAATTAATCAAATATATCATAGTTTTTTTCGTTGTATATTTTTCTTTTTTAGAAACTATAGGTTAAAGAGTTTATCGATCACGGGCGAATTTCAGAAAAAAAAAATATAAAATAAAAGAATAGTCACATGGTAAGATTCTTTGTATGATTTATTGGTTTAGATTGTTGTTCTCACATGCATAATTGTCACGTATTGGAAATATTCCAATATTCGAATATTCCAATATTCCTAGGACACATGAAAATATTGTGTATTCTTTTTCTATTATTATTTCAGGCATAAAGTTTTTATTTTATTTTGGTATTTATTTAAATATTTTATTTTATCGAATTTTAATTATGGGCAGGTGTTCTTCCTGTGTTTGCTTGCGGGTTGCAGCATCTGTTGGTTCAACACAGTCTGTTTCGTCCTTTGCATTCGGAACTTTTCTGCTAATCGGCCATTGGCATTATCTCTCACTGTCAGCTTCAATGGCGTCAGTGCAGCTTTCTACACGCTCGCCGGCAACGCCATTAATCCCTCTTCGCCTCCCATTTACCTCCTTCTCAACGCTCTCATTCCGCTTCTCACTTCCATTGCCGCTTTCCTCCCCGTCCTCCATCAACCGCCGCTGCACTCGCTTTCTCTCTCCTCTGATGCCGTTCAGCGAGACTCTCTCATTTTTCTCATCTTGAATTTCCTTGCTGTCGCCGTTGGGATCTACCTTCTACTCTTCGGCTCTGTCACTTCCGCCGATCCGATGATGGCGAGGCTTCTCTTCATTGGAGCCATCGTCCTTCTCGTGTTGCCTCTTTGTATTCCCGGCGTTGTTTACGCTAATGATTGGTTCCACCGAACCGTTAACTCGAGCTTCAGCCTTGACGGATCTAGCTTCATTCTTGTCCACGACGAAGATCTTGAGTTTCACAAGGAGCTGCTTAGTCGTGAAGCGAATGGAAGCTTTGGCAATGGCGGATCTCAGTTATTTTCTGAATCTGCTTCGCTTATTGACGGTGAGATTGAATCGAAAGGCTGTTTTCCGAAATTGGTTGAGAAGGATCAATTGGCGATGCTTGGGGAAGAGCATCCGTCGAGCCGATTGGTGAAACGACTGGATTTTTGGCTGTATTTCACCGCTTACATTTGCGGTGGCACAATTGGACTTGTTTACAGTAACAATATAGGACAGATTGCGCAATCGCTAGGGTTGAGTTCAAGAACCAATGCCATCGTAACACTCTACTCCTCCTTCTCCTTCTTCGGCCGATTGCTCTCGGCCGTACCGGACTACATTCGAGCGTAATTTATAATCGATTCTATTCAATTTCTCCACTGTTTCCTCTGCTTTTCCAAACACTCACACCGCCAATTTTATTTTCTCCGCAGGAAATTATATTTCGCAAGAACAGGATGGCTTTCGATTGCTCTAATTCCAACTCCTATAGCTTTCTTTCTACTATCGGCGTCGTCGAGCACGGTGACGGTGTACATAGGCACGGCACTGATCGGTCTGAGCTCCGGCTTCATATTCGCGGCGGCAGTTTCCATTACGGCGGAGCTGTTCGGGCCAAATAGCTTAGGCGTTAACCACAACATTCTGATCACAAACATTCCAATCGGATCTCTTCTGTACGGCATGTTTGCAGCGGTGGTATACGATTCGCAGGGATGGAACTCCGGGCATGGAGAGGCGGTTGTGTGCATGGGAAGAAGGTGCTATTTCTTGACTTTTGTATTTTGTGGATGCATTTCTGTTGTAGGATTGGTTTCTAGCGTGTTACTGTTCTTGAGAACCAGACACGCTTATGATCACTTCGAAAGTAGTAGGATTTCCAGTAGTTCAAGTAGATTGTATTAATTTATCTCACTGTTATCATTATTCATTCCATACCTGATTTTAGTTCCTTTTTTTTTTCCTTCTTTTTTTCTCAATGCATCATTTCATTTCAATTGTTCTG

mRNA sequence

AGTAGTTCCAGAGGCAACTGCCGGGAGGAGGAGGAGGAGGAGGAGGACGGAAGAGGAATCCGCCTCCGCCTCAGCCGCCGCCATCGTCGAGATCGAGGTCGATTTGTGATGGCCGGCCAGTCGCGGAAGTGGCTGGTTCTGGTGGCGACGATATGGATTCAAGCATTCACCGGCACGAACTTCGATTTCTCGGCCTATTCTTCGAGATTGAAATCGGTATTAGGCATTTCTCAGGTTCAACTTAATTACCTCGCCACCGCCTCCGATCTAGGCAAGGTTTTCGGCTGGTCCTCTGGATTAGCTCTGCTTCACCTACCGTTGCCGGTGGTTCTGTTCATCGCCGCTTTCTTAGGACTAATCGGTTACGGCTTCCAATGGCTCCTAATCGCCGATTTCATCTCCTTGCCCTATTTCCTGGTGTTCTTCCTGTGTTTGCTTGCGGGTTGCAGCATCTGTTGGTTCAACACAGTCTGTTTCGTCCTTTGCATTCGGAACTTTTCTGCTAATCGGCCATTGGCATTATCTCTCACTGTCAGCTTCAATGGCGTCAGTGCAGCTTTCTACACGCTCGCCGGCAACGCCATTAATCCCTCTTCGCCTCCCATTTACCTCCTTCTCAACGCTCTCATTCCGCTTCTCACTTCCATTGCCGCTTTCCTCCCCGTCCTCCATCAACCGCCGCTGCACTCGCTTTCTCTCTCCTCTGATGCCGTTCAGCGAGACTCTCTCATTTTTCTCATCTTGAATTTCCTTGCTGTCGCCGTTGGGATCTACCTTCTACTCTTCGGCTCTGTCACTTCCGCCGATCCGATGATGGCGAGGCTTCTCTTCATTGGAGCCATCGTCCTTCTCGTGTTGCCTCTTTGTATTCCCGGCGTTGTTTACGCTAATGATTGGTTCCACCGAACCGTTAACTCGAGCTTCAGCCTTGACGGATCTAGCTTCATTCTTGTCCACGACGAAGATCTTGAGTTTCACAAGGAGCTGCTTAGTCGTGAAGCGAATGGAAGCTTTGGCAATGGCGGATCTCAGTTATTTTCTGAATCTGCTTCGCTTATTGACGGTGAGATTGAATCGAAAGGCTGTTTTCCGAAATTGGTTGAGAAGGATCAATTGGCGATGCTTGGGGAAGAGCATCCGTCGAGCCGATTGGTGAAACGACTGGATTTTTGGCTGTATTTCACCGCTTACATTTGCGGTGGCACAATTGGACTTGTTTACAGTAACAATATAGGACAGATTGCGCAATCGCTAGGGTTGAGTTCAAGAACCAATGCCATCGTAACACTCTACTCCTCCTTCTCCTTCTTCGGCCGATTGCTCTCGGCCGTACCGGACTACATTCGAGCGAAATTATATTTCGCAAGAACAGGATGGCTTTCGATTGCTCTAATTCCAACTCCTATAGCTTTCTTTCTACTATCGGCGTCGTCGAGCACGGTGACGGTGTACATAGGCACGGCACTGATCGGTCTGAGCTCCGGCTTCATATTCGCGGCGGCAGTTTCCATTACGGCGGAGCTGTTCGGGCCAAATAGCTTAGGCGTTAACCACAACATTCTGATCACAAACATTCCAATCGGATCTCTTCTGTACGGCATGTTTGCAGCGGTGGTATACGATTCGCAGGGATGGAACTCCGGGCATGGAGAGGCGGTTGTGTGCATGGGAAGAAGGTGCTATTTCTTGACTTTTGTATTTTGTGGATGCATTTCTGTTGTAGGATTGGTTTCTAGCGTGTTACTGTTCTTGAGAACCAGACACGCTTATGATCACTTCGAAAGTAGTAGGATTTCCAGTAGTTCAAGTAGATTGTATTAATTTATCTCACTGTTATCATTATTCATTCCATACCTGATTTTAGTTCCTTTTTTTTTTCCTTCTTTTTTTCTCAATGCATCATTTCATTTCAATTGTTCTG

Coding sequence (CDS)

ATGGCCGGCCAGTCGCGGAAGTGGCTGGTTCTGGTGGCGACGATATGGATTCAAGCATTCACCGGCACGAACTTCGATTTCTCGGCCTATTCTTCGAGATTGAAATCGGTATTAGGCATTTCTCAGGTTCAACTTAATTACCTCGCCACCGCCTCCGATCTAGGCAAGGTTTTCGGCTGGTCCTCTGGATTAGCTCTGCTTCACCTACCGTTGCCGGTGGTTCTGTTCATCGCCGCTTTCTTAGGACTAATCGGTTACGGCTTCCAATGGCTCCTAATCGCCGATTTCATCTCCTTGCCCTATTTCCTGGTGTTCTTCCTGTGTTTGCTTGCGGGTTGCAGCATCTGTTGGTTCAACACAGTCTGTTTCGTCCTTTGCATTCGGAACTTTTCTGCTAATCGGCCATTGGCATTATCTCTCACTGTCAGCTTCAATGGCGTCAGTGCAGCTTTCTACACGCTCGCCGGCAACGCCATTAATCCCTCTTCGCCTCCCATTTACCTCCTTCTCAACGCTCTCATTCCGCTTCTCACTTCCATTGCCGCTTTCCTCCCCGTCCTCCATCAACCGCCGCTGCACTCGCTTTCTCTCTCCTCTGATGCCGTTCAGCGAGACTCTCTCATTTTTCTCATCTTGAATTTCCTTGCTGTCGCCGTTGGGATCTACCTTCTACTCTTCGGCTCTGTCACTTCCGCCGATCCGATGATGGCGAGGCTTCTCTTCATTGGAGCCATCGTCCTTCTCGTGTTGCCTCTTTGTATTCCCGGCGTTGTTTACGCTAATGATTGGTTCCACCGAACCGTTAACTCGAGCTTCAGCCTTGACGGATCTAGCTTCATTCTTGTCCACGACGAAGATCTTGAGTTTCACAAGGAGCTGCTTAGTCGTGAAGCGAATGGAAGCTTTGGCAATGGCGGATCTCAGTTATTTTCTGAATCTGCTTCGCTTATTGACGGTGAGATTGAATCGAAAGGCTGTTTTCCGAAATTGGTTGAGAAGGATCAATTGGCGATGCTTGGGGAAGAGCATCCGTCGAGCCGATTGGTGAAACGACTGGATTTTTGGCTGTATTTCACCGCTTACATTTGCGGTGGCACAATTGGACTTGTTTACAGTAACAATATAGGACAGATTGCGCAATCGCTAGGGTTGAGTTCAAGAACCAATGCCATCGTAACACTCTACTCCTCCTTCTCCTTCTTCGGCCGATTGCTCTCGGCCGTACCGGACTACATTCGAGCGAAATTATATTTCGCAAGAACAGGATGGCTTTCGATTGCTCTAATTCCAACTCCTATAGCTTTCTTTCTACTATCGGCGTCGTCGAGCACGGTGACGGTGTACATAGGCACGGCACTGATCGGTCTGAGCTCCGGCTTCATATTCGCGGCGGCAGTTTCCATTACGGCGGAGCTGTTCGGGCCAAATAGCTTAGGCGTTAACCACAACATTCTGATCACAAACATTCCAATCGGATCTCTTCTGTACGGCATGTTTGCAGCGGTGGTATACGATTCGCAGGGATGGAACTCCGGGCATGGAGAGGCGGTTGTGTGCATGGGAAGAAGGTGCTATTTCTTGACTTTTGTATTTTGTGGATGCATTTCTGTTGTAGGATTGGTTTCTAGCGTGTTACTGTTCTTGAGAACCAGACACGCTTATGATCACTTCGAAAGTAGTAGGATTTCCAGTAGTTCAAGTAGATTGTATTAA

Protein sequence

MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGWSSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNTVCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSIAAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLLFIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANGSFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTAYICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFARTGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGVNHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVVGLVSSVLLFLRTRHAYDHFESSRISSSSSRLY
Homology
BLAST of CmoCh16G005650 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 4.2e-165
Identity = 328/571 (57.44%), Postives = 407/571 (71.28%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           M G+ RKW VLVA IWIQA TGTNFDFSAYSS LKSVLGISQV+LNYLA ASDLGK FGW
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSG+AL + PL VVLF AA +G +GYG QWL+I + I+LPY LVF  CLLAG SICWFNT
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWFNT 156

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
            CF+LCIR+F  NR LALSLTVSFNG+SAA Y+LA NAINPSS  +YLLLN+L+PL+ S 
Sbjct: 157 ACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSF 216

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
           AA  PVL +P L + +   D+ + DS +F ILN LAV    +LLL  S TS+    ARL 
Sbjct: 217 AALYPVLTKPSLDT-TPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSS----ARLN 276

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300
           FIGA+VLLV PLC P +VYA D+F   +N+  + + S +++++ ++L+  K  +S +   
Sbjct: 277 FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG- 336

Query: 301 SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 360
                                E  G      ++     LG+EH    L+ RL+FWLY+ A
Sbjct: 337 --------------------YEHMG----TAKEGNTVRLGDEHSFRLLISRLEFWLYYIA 396

Query: 361 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 420
           Y CGGTIGLVYSNN+GQIAQSLG +S T  +VT+YSSFSFFGRLLSA PD++  +    R
Sbjct: 397 YFCGGTIGLVYSNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMHKRFRLTR 456

Query: 421 TGWLSIALIPTPIAFFLLSASSSTVT-VYIGTALIGLSSGFIFAAAVSITAELFGPNSLG 480
           TGW +IAL+PTPIAFFLL+ SSS  T +   TALIGLSSGFIFAAAVSIT++LFGPNS+G
Sbjct: 457 TGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVG 516

Query: 481 VNHNILITNIPIGSLLYGMFAAVVYD---SQGWNSGHGEAVVCMGRRCYFLTFVFCGCIS 540
           VNHNILITNIPIGSLLYG  AA +Y+   S        +++VC+GR CYF TFVF GC+S
Sbjct: 517 VNHNILITNIPIGSLLYGYIAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLS 575

Query: 541 VVGLVSSVLLFLRTRHAYDHFESSRISSSSS 568
           ++G+VSS+ L++RT+  Y   E  ++S +SS
Sbjct: 577 ILGVVSSLSLYIRTKPVYHRLEQDKVSLTSS 575

BLAST of CmoCh16G005650 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 7.6e-05
Identity = 52/190 (27.37%), Postives = 84/190 (44.21%), Query Frame = 0

Query: 355 WLYFTAYICGGTIGLVYSNNIGQIAQSLGL----SSRTNAIVTLYSSFSFFGRLLSAVPD 414
           WL+   ++  G    ++ NN+G I  ++ +    S   +  V+L+++FS   RL+     
Sbjct: 275 WLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSS 334

Query: 415 YIRAKLYFARTGWLSIALIPTPIAFFLLSASSSTVTVY-------IGTALIGLSSGFIFA 474
               + + +R   LS+  I    A   L   S   TV+       + T + G S G  F 
Sbjct: 335 E-AMESHVSRPVLLSV--IALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFT 394

Query: 475 AAVSITAELFGPNSLGVNHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRR 534
              +I  +++G  +LG      I  + +GSL YG+  A VYD+         + VC G  
Sbjct: 395 LVPTIVTKVWGIANLGTIWGSFILALAVGSLGYGLLFAKVYDAASEVGVGSMSQVCSGVH 454

BLAST of CmoCh16G005650 vs. ExPASy TrEMBL
Match: A0A6J1ETH8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111437588 PE=4 SV=1)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW
Sbjct: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT
Sbjct: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
           VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI
Sbjct: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
           AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL
Sbjct: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300
           FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG
Sbjct: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300

Query: 301 SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 360
           SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA
Sbjct: 301 SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 360

Query: 361 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 420
           YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR
Sbjct: 361 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 420

Query: 421 TGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGV 480
           TGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGV
Sbjct: 421 TGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGV 480

Query: 481 NHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVVGL 540
           NHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVVGL
Sbjct: 481 NHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVVGL 540

Query: 541 VSSVLLFLRTRHAYDHFESSRISSSSSRLY 571
           VSSVLLFLRTRHAYDHFESSRISSSSSRLY
Sbjct: 541 VSSVLLFLRTRHAYDHFESSRISSSSSRLY 570

BLAST of CmoCh16G005650 vs. ExPASy TrEMBL
Match: A0A6J1JBB1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483444 PE=4 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 563/570 (98.77%), Postives = 565/570 (99.12%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASD+GKVFGW
Sbjct: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDIGKVFGW 60

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT
Sbjct: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
           VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI
Sbjct: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
           AAFLPVLHQPPL SLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL
Sbjct: 181 AAFLPVLHQPPLQSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300
           FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG
Sbjct: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300

Query: 301 SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 360
           SFGNGGSQL SESASLIDGEIESK CFPKL+EKDQLAMLGEEHPSSRLVKRLDFWLYFTA
Sbjct: 301 SFGNGGSQLLSESASLIDGEIESKSCFPKLIEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 360

Query: 361 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 420
           YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR
Sbjct: 361 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 420

Query: 421 TGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGV 480
           TGWLSIALIPTPIAFFLLSASSS VTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGV
Sbjct: 421 TGWLSIALIPTPIAFFLLSASSSMVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGV 480

Query: 481 NHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVVGL 540
           NHNILITNIPIGSLLYGMFAAVVYDSQG NSGHGEAVVCMGRRCYFLTFVFCGCISVVGL
Sbjct: 481 NHNILITNIPIGSLLYGMFAAVVYDSQGRNSGHGEAVVCMGRRCYFLTFVFCGCISVVGL 540

Query: 541 VSSVLLFLRTRHAYDHFESSRISSSSSRLY 571
           VSSVLLFLRTRHAYDHFESSRISSSSSRLY
Sbjct: 541 VSSVLLFLRTRHAYDHFESSRISSSSSRLY 570

BLAST of CmoCh16G005650 vs. ExPASy TrEMBL
Match: A0A1S3BTD8 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493060 PE=4 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.0e-291
Identity = 529/572 (92.48%), Postives = 549/572 (95.98%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSS+LKSVLGISQVQLNYLATASDLGKVFGW
Sbjct: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSKLKSVLGISQVQLNYLATASDLGKVFGW 60

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSGLALLHLPLPVV+FIAAFLG IGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT
Sbjct: 61  SSGLALLHLPLPVVMFIAAFLGFIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
           VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLA NAINPSSPPIYLLLN+LIPLLTSI
Sbjct: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAANAINPSSPPIYLLLNSLIPLLTSI 180

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
           A FLPVLHQPPLHSLSLSSDAV RDSLIFLILNFLA+ VGIYLLLFGSVTSADPM+ARLL
Sbjct: 181 AVFLPVLHQPPLHSLSLSSDAVHRDSLIFLILNFLAIVVGIYLLLFGSVTSADPMIARLL 240

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKE-LLSREAN 300
           FIGAIVLL+LPLCIPG+VYANDWFHRTVNSSF LDGS+FILVHDEDLEFHKE LLS EAN
Sbjct: 241 FIGAIVLLILPLCIPGIVYANDWFHRTVNSSFHLDGSNFILVHDEDLEFHKELLLSLEAN 300

Query: 301 GSFGNGGSQLFSESASLIDGEIE-SKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYF 360
           GSFGNG + L SESASLIDGEIE SKGC  KL+E DQLAMLGEEH SSRLV+RLDFWLYF
Sbjct: 301 GSFGNGETSLLSESASLIDGEIESSKGCLRKLIELDQLAMLGEEHSSSRLVQRLDFWLYF 360

Query: 361 TAYICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYF 420
            AYICGGTIGLVYSNNIGQIAQSLGL+SRT AIVTLYSSFSFFGRLLSAVPDYIRAKLYF
Sbjct: 361 IAYICGGTIGLVYSNNIGQIAQSLGLNSRTKAIVTLYSSFSFFGRLLSAVPDYIRAKLYF 420

Query: 421 ARTGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSL 480
           ARTGWLSIALIPTPIAFFLLSASS+ ++VYIGTALIGLSSGFIFAAAVSITAELFGPNSL
Sbjct: 421 ARTGWLSIALIPTPIAFFLLSASSTEMSVYIGTALIGLSSGFIFAAAVSITAELFGPNSL 480

Query: 481 GVNHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVV 540
           GVNHNILITNIPIGSLLYGM AAVVYDSQG +S +GEA+VCMGRRCYFLTFVFCGCISVV
Sbjct: 481 GVNHNILITNIPIGSLLYGMLAAVVYDSQGRSSDNGEAIVCMGRRCYFLTFVFCGCISVV 540

Query: 541 GLVSSVLLFLRTRHAYDHFESSRISSSSSRLY 571
           GLVSSVLLFLRTRHAYD FESSRISSS++RLY
Sbjct: 541 GLVSSVLLFLRTRHAYDRFESSRISSSTNRLY 572

BLAST of CmoCh16G005650 vs. ExPASy TrEMBL
Match: A0A6J1C4X1 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Momordica charantia OX=3673 GN=LOC111008508 PE=4 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.2e-290
Identity = 519/570 (91.05%), Postives = 546/570 (95.79%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           MAGQSRKWL+LVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW
Sbjct: 1   MAGQSRKWLILVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSGLALLHLPLP+VLFIAAF+GLIGYG QWL+IADFISLPYFLVFFLCLLAGCSICWFNT
Sbjct: 61  SSGLALLHLPLPLVLFIAAFMGLIGYGLQWLVIADFISLPYFLVFFLCLLAGCSICWFNT 120

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
           VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI
Sbjct: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
           AA  P+L QPPLHSLSLSSDAVQRDS+IFL LNFLAV VGIYLLLFGS+ S+DPM+AR+L
Sbjct: 181 AALFPILRQPPLHSLSLSSDAVQRDSIIFLFLNFLAVIVGIYLLLFGSMASSDPMIARIL 240

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300
           FIGAI LLVLPLCIPGVVYA +WF RTVNSSF LDGS FILVHDEDLEFHKEL+SREANG
Sbjct: 241 FIGAICLLVLPLCIPGVVYAKNWFRRTVNSSFRLDGSGFILVHDEDLEFHKELISREANG 300

Query: 301 SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 360
           SFGNGGS L SESASL DGE+E+KGCF KL+EKDQLAMLGEEHPSSRLVKR+DFWLYF A
Sbjct: 301 SFGNGGSLLLSESASLNDGEVEAKGCFRKLIEKDQLAMLGEEHPSSRLVKRVDFWLYFIA 360

Query: 361 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 420
           YICGGTIGLVYSNNIGQI+QSLGLSSRTNAIVTLYS+FSFFGRLLSAVPDYIRAKLYFAR
Sbjct: 361 YICGGTIGLVYSNNIGQISQSLGLSSRTNAIVTLYSAFSFFGRLLSAVPDYIRAKLYFAR 420

Query: 421 TGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLGV 480
           TGWLSIALIPTPIAFFLL+ASS+TVTVYIGTAL+G+SSGFIFAAAVSITAELFGPNSLGV
Sbjct: 421 TGWLSIALIPTPIAFFLLAASSTTVTVYIGTALVGMSSGFIFAAAVSITAELFGPNSLGV 480

Query: 481 NHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVVGL 540
           NHNILITNIPIGSLLYGM AAVVYDS G +SGHGEAVVCMGRRCYF TF+FCGC+SVVGL
Sbjct: 481 NHNILITNIPIGSLLYGMLAAVVYDSHG-SSGHGEAVVCMGRRCYFFTFLFCGCVSVVGL 540

Query: 541 VSSVLLFLRTRHAYDHFESSRISSSSSRLY 571
           VSSVLLF RTRHAYDHFE +RI SSS+RLY
Sbjct: 541 VSSVLLFFRTRHAYDHFERNRIFSSSNRLY 569

BLAST of CmoCh16G005650 vs. ExPASy TrEMBL
Match: A0A0A0L001 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G055450 PE=4 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.8e-289
Identity = 526/572 (91.96%), Postives = 543/572 (94.93%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSS+LKSVLGISQVQLNYLATASDLGKVFGW
Sbjct: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSKLKSVLGISQVQLNYLATASDLGKVFGW 60

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSGLALLHLPLP+ +FIAAFLG IGYGFQWLLI DFISLPYFLVFFLCLLAGCSICWFNT
Sbjct: 61  SSGLALLHLPLPMAMFIAAFLGFIGYGFQWLLIVDFISLPYFLVFFLCLLAGCSICWFNT 120

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
           VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLL SI
Sbjct: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLISI 180

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
             FLPVLHQPPLHSLSL SDAV RDSLIFLILNFLA+ VGIYLLLFGSVTSADPM+ARLL
Sbjct: 181 VVFLPVLHQPPLHSLSLPSDAVHRDSLIFLILNFLAIIVGIYLLLFGSVTSADPMIARLL 240

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKE-LLSREAN 300
           FIGAIVLL+LPLCIPG+VYANDWFHRTVNSSF LDGS+FILVHDEDLEFHKE LLS E+N
Sbjct: 241 FIGAIVLLILPLCIPGIVYANDWFHRTVNSSFRLDGSNFILVHDEDLEFHKELLLSLESN 300

Query: 301 GSFGNGGSQLFSESASLIDGEIE-SKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYF 360
           GSFGNG S L SESASLIDGE E SKGC  KL+E DQLAMLGEEH SSRLVKRLDFWLYF
Sbjct: 301 GSFGNGESPLLSESASLIDGETEPSKGCLRKLIEIDQLAMLGEEHSSSRLVKRLDFWLYF 360

Query: 361 TAYICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYF 420
            AYICGGTIGLVYSNNIGQIAQSLGLSSRT AIVTLYSSFSFFGRLLSAVPDYIRAKLYF
Sbjct: 361 IAYICGGTIGLVYSNNIGQIAQSLGLSSRTKAIVTLYSSFSFFGRLLSAVPDYIRAKLYF 420

Query: 421 ARTGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSL 480
           ARTGWLSIALIPTPIAFFLLSASS+ + VYIGTALIGLSSGFIFAAAVSITAELFGPNSL
Sbjct: 421 ARTGWLSIALIPTPIAFFLLSASSTAMAVYIGTALIGLSSGFIFAAAVSITAELFGPNSL 480

Query: 481 GVNHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVV 540
           GVNHNILITNIPIGSLLYGM AAVVYDSQG +S +GEA+VCMGRRCYFLTFVFCGCISVV
Sbjct: 481 GVNHNILITNIPIGSLLYGMLAAVVYDSQGKSSDNGEAIVCMGRRCYFLTFVFCGCISVV 540

Query: 541 GLVSSVLLFLRTRHAYDHFESSRISSSSSRLY 571
           GLVSSVLLFLRTRHAYD FESSRISSS++RLY
Sbjct: 541 GLVSSVLLFLRTRHAYDRFESSRISSSTNRLY 572

BLAST of CmoCh16G005650 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 727.2 bits (1876), Expect = 9.9e-210
Identity = 388/575 (67.48%), Postives = 455/575 (79.13%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           MAGQSRKW++LVATIWIQAFTGTNFDFSAYSS LKSVLGISQVQLNYLA ASDLGKVFGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSGLAL++ PL  VLF AAF+G +GYG QWL+I  FISLPY +VF  CLLAG SICWFNT
Sbjct: 61  SSGLALMYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLPYIMVFLCCLLAGLSICWFNT 120

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
           VCFVLCI NF ANR LALSLTVSFNGVSAA YTLA NAINP+SP +YLLLNALIPL+ S 
Sbjct: 121 VCFVLCISNFPANRSLALSLTVSFNGVSAALYTLAYNAINPTSPELYLLLNALIPLIVSF 180

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
            A +P+L QPP     L  D V+RDSL+FL+LN LA   G+YLLLFGS  S+D   ARLL
Sbjct: 181 TAIIPILRQPPFE--PLPPDGVRRDSLMFLLLNILAALNGVYLLLFGS-NSSDLTSARLL 240

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300
           F GAI+LLV PLCIPG+V A +W++RT+++SF L+GS FILV  ++LE HK +L+ EAN 
Sbjct: 241 FGGAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEAN- 300

Query: 301 SFGNGGSQLFSE-------SASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLD 360
                G QL S+        +  ++ E   + C  KL+ +DQL  LG EH  S L+ R D
Sbjct: 301 ---REGYQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSD 360

Query: 361 FWLYFTAYICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIR 420
           FWLY+  Y CGGTIGLVYSNN+GQIAQSLG SS T  +VTLYS+FSFFGRLLSA PDYIR
Sbjct: 361 FWLYYITYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYIR 420

Query: 421 AKLYFARTGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELF 480
           AK+YFARTGWL+IAL+PTP A FLL++S +   +  GTAL+GLSSGFIFAAAVSIT+ELF
Sbjct: 421 AKVYFARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELF 480

Query: 481 GPNSLGVNHNILITNIPIGSLLYGMFAAVVYDSQGW---NSGHGEAVVCMGRRCYFLTFV 540
           GPNS+GVNHNILITNIPIGSL+YG  AA+VYDS G+    S   E+VVCMGR CY+LTFV
Sbjct: 481 GPNSVGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYYLTFV 540

Query: 541 FCGCISVVGLVSSVLLFLRTRHAYDHFESSRISSS 566
           + GC+S++GL SS++LF+RTR AY  FE +RISS+
Sbjct: 541 WWGCLSLLGLGSSLVLFIRTRRAYQRFEQARISSN 568

BLAST of CmoCh16G005650 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 719.9 bits (1857), Expect = 1.6e-207
Identity = 386/573 (67.36%), Postives = 448/573 (78.18%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           MAGQSRKW++LVATIWIQAFTGTNFDFS YSS LKSVLGISQVQLNYLA ASDLGKVFGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSGLALL+ PL  VLF AA +G +GYG QWL+I + ISLPY LVF  CLLAG SICWFNT
Sbjct: 61  SSGLALLYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLPYILVFLCCLLAGLSICWFNT 120

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
           VCFVLCIRNF ANR LALSLTVSFNGVSAA YTLA NAINP S  +YLLLNAL+PL  S 
Sbjct: 121 VCFVLCIRNFPANRSLALSLTVSFNGVSAALYTLAYNAINPVSTELYLLLNALVPLFVSF 180

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
           AA +P+L QPPL    L  D V+RDSL+FL+LN LAV  G+YLLLF S TS D   ARLL
Sbjct: 181 AALIPILRQPPLE--PLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTS-DVTSARLL 240

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300
           F G+I+LL+LPLC+PG+VYA +W+   ++SSF L+GS FILV  ++LE HK +++REA+ 
Sbjct: 241 FGGSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREASL 300

Query: 301 SFGNGGSQLFSESASLIDGEIESKG--------CFPKLVEKDQLAMLGEEHPSSRLVKRL 360
                G QL ++         + K         C  K++ ++QL MLGEEHP S L+ R 
Sbjct: 301 E----GYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRS 360

Query: 361 DFWLYFTAYICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYI 420
           DFWLY+ AY CGGTIGLVYSNN+GQIAQSLG SS T  +VTLYSSFSFFGRLLSA PDYI
Sbjct: 361 DFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI 420

Query: 421 RAKLYFARTGWLSIALIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAEL 480
           RAK+YFARTGWL++AL+PT IA FLL++S S   +  GTALIGLSSGFIFAAAVSIT+EL
Sbjct: 421 RAKVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSEL 480

Query: 481 FGPNSLGVNHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFC 540
           FGPNS+GVNHNILITNIPIGSL+YG  AA+VY+S        E+V+CMGR CY  TF++ 
Sbjct: 481 FGPNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMWW 540

Query: 541 GCISVVGLVSSVLLFLRTRHAYDHFESSRISSS 566
           GC+SV+GL SSV+LFLRTR AY  FE  RI+SS
Sbjct: 541 GCLSVIGLASSVVLFLRTRRAYQRFEQDRITSS 566

BLAST of CmoCh16G005650 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 582.8 bits (1501), Expect = 3.0e-166
Identity = 328/571 (57.44%), Postives = 407/571 (71.28%), Query Frame = 0

Query: 1   MAGQSRKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGW 60
           M G+ RKW VLVA IWIQA TGTNFDFSAYSS LKSVLGISQV+LNYLA ASDLGK FGW
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 61  SSGLALLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNT 120
           SSG+AL + PL VVLF AA +G +GYG QWL+I + I+LPY LVF  CLLAG SICWFNT
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWFNT 156

Query: 121 VCFVLCIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSSPPIYLLLNALIPLLTSI 180
            CF+LCIR+F  NR LALSLTVSFNG+SAA Y+LA NAINPSS  +YLLLN+L+PL+ S 
Sbjct: 157 ACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSF 216

Query: 181 AAFLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLL 240
           AA  PVL +P L + +   D+ + DS +F ILN LAV    +LLL  S TS+    ARL 
Sbjct: 217 AALYPVLTKPSLDT-TPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSS----ARLN 276

Query: 241 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 300
           FIGA+VLLV PLC P +VYA D+F   +N+  + + S +++++ ++L+  K  +S +   
Sbjct: 277 FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG- 336

Query: 301 SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 360
                                E  G      ++     LG+EH    L+ RL+FWLY+ A
Sbjct: 337 --------------------YEHMG----TAKEGNTVRLGDEHSFRLLISRLEFWLYYIA 396

Query: 361 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 420
           Y CGGTIGLVYSNN+GQIAQSLG +S T  +VT+YSSFSFFGRLLSA PD++  +    R
Sbjct: 397 YFCGGTIGLVYSNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMHKRFRLTR 456

Query: 421 TGWLSIALIPTPIAFFLLSASSSTVT-VYIGTALIGLSSGFIFAAAVSITAELFGPNSLG 480
           TGW +IAL+PTPIAFFLL+ SSS  T +   TALIGLSSGFIFAAAVSIT++LFGPNS+G
Sbjct: 457 TGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVG 516

Query: 481 VNHNILITNIPIGSLLYGMFAAVVYD---SQGWNSGHGEAVVCMGRRCYFLTFVFCGCIS 540
           VNHNILITNIPIGSLLYG  AA +Y+   S        +++VC+GR CYF TFVF GC+S
Sbjct: 517 VNHNILITNIPIGSLLYGYIAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLS 575

Query: 541 VVGLVSSVLLFLRTRHAYDHFESSRISSSSS 568
           ++G+VSS+ L++RT+  Y   E  ++S +SS
Sbjct: 577 ILGVVSSLSLYIRTKPVYHRLEQDKVSLTSS 575

BLAST of CmoCh16G005650 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 513.5 bits (1321), Expect = 2.2e-145
Identity = 299/570 (52.46%), Postives = 380/570 (66.67%), Query Frame = 0

Query: 6   RKWLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGWSSGLA 65
           R+W VLVA IWIQAFTGTNFDFSAYSS +KS +G+SQ +LNY+A ASDLGK  GWSSG A
Sbjct: 22  RRWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFA 81

Query: 66  LLHLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNTVCFVL 125
           + + P+  VLF AA +GL+GYG QWL IAD I LPY LV   C LAG SICWFNTV F+L
Sbjct: 82  IAYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLPYSLVLVCCSLAGLSICWFNTVSFIL 141

Query: 126 CIRNFSANRPLALSLTVSFNGVSAAFYTLAGNAI---NPSSPPIYLLLNALIPLLTSIAA 185
           CIR+F AN  LALSL VSFNG+SAA YTL   AI   + +S  IYLLLN+LIPL+ S+ A
Sbjct: 142 CIRHFKANHSLALSLVVSFNGISAALYTLGHEAISGKSSASSDIYLLLNSLIPLIVSVLA 201

Query: 186 FLPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLL--FGSVTSADPMMARLL 245
             PVL  P  +S    +     ++ IF++ N LA+    YLLL   G+  ++ P   R  
Sbjct: 202 LWPVLTNP--NSSETDTTRTHDETRIFVVFNVLALVTCFYLLLPSSGTYLASSP---RWH 261

Query: 246 FIGAIVLLVLPLCIPGVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANG 305
           F+GAI LL+ PLC+P +    D+ HR + S F    S +  V+ E+ +  K + S+++N 
Sbjct: 262 FLGAIFLLLFPLCVPFL----DYIHRALESCFHHHSSGYAAVNIEEPKILK-IKSQKSNA 321

Query: 306 SFGNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLGEEHPSSRLVKRLDFWLYFTA 365
                                          E+     LG+EH    LV++L+FWLY+ A
Sbjct: 322 E------------------------------EESDQVRLGDEHSLGMLVRKLEFWLYYVA 381

Query: 366 YICGGTIGLVYSNNIGQIAQSLG-LSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKL-YF 425
           Y CGGTIGLVYSNN+GQIAQSLG  SS   ++VTL+S+FSF GRLLS+ PD+ R KL Y 
Sbjct: 382 YFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTLFSAFSFLGRLLSSAPDFTRKKLKYL 441

Query: 426 ARTGWLSIALIPTPIAFFLLSAS---SSTVTVYIGTALIGLSSGFIFAAAVSITAELFGP 485
            RTGW +I+L+PTP+AFF+L+ S   + T  + + TALIGLSSGF+FAAAVSIT+ELFG 
Sbjct: 442 TRTGWFTISLLPTPLAFFILAYSPKTNQTALLEVATALIGLSSGFVFAAAVSITSELFGR 501

Query: 486 NSLGVNHNILITNIPIGSLLYGMFAAVVYD---SQGWNSGHGEAVVCMGRRCYFLTFVFC 545
           NS+GVN NILITNIPIGSL YG  A  VYD   S G  S   ++VVC+GR+CYF+TF+F 
Sbjct: 502 NSVGVNQNILITNIPIGSLFYGYMAGSVYDTNASLGRKSVVADSVVCVGRKCYFVTFLFW 551

Query: 546 GCISVVGLVSSVLLFLRTRHAYDHFESSRI 563
           GC+SV+G V S+ LF+RTR  Y   + +R+
Sbjct: 562 GCLSVLGFVCSLFLFIRTRPVYHRLKLNRM 551

BLAST of CmoCh16G005650 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 9.3e-75
Identity = 197/555 (35.50%), Postives = 299/555 (53.87%), Query Frame = 0

Query: 8   WLVLVATIWIQAFTGTNFDFSAYSSRLKSVLGISQVQLNYLATASDLGKVFGWSSGLALL 67
           WL LVA +W+Q+F GTN  F AYSS+LK +L ISQ +LNYL+ ASD GKV G+ SG+A +
Sbjct: 8   WLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGIAAV 67

Query: 68  HLPLPVVLFIAAFLGLIGYGFQWLLIADFISLPYFLVFFLCLLAGCSICWFNTVCFVLCI 127
           +LPLP+VL     LG  GYG Q+L I   I                 ICW NT C+++ I
Sbjct: 68  YLPLPLVLLAGGSLGFAGYGLQYLSIIKKI-----------------ICWINTACYIVAI 127

Query: 128 RNFSANRPLALSLTVSFNGVSAAFYTLAGNAINPSS----PPIYLLLNALIPLLTSIAAF 187
            +F  NR +A+ +T S+ G+S   YT   ++   +S       YLLLN+L+PL+  +   
Sbjct: 128 NSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVACLVTA 187

Query: 188 LPVLHQPPLHSLSLSSDAVQRDSLIFLILNFLAVAVGIYLLLFGSVTSADPMMARLLFIG 247
             ++      ++S S D      + F++L  L +A GIY +     TS   + A L+ +G
Sbjct: 188 PMLMRHGGDKTMSYSKDV----KVGFIVLFVLTIATGIYAV----ATSLVSVPAVLVLVG 247

Query: 248 AIVLLVLPLCIP-GVVYANDWFHRTVNSSFSLDGSSFILVHDEDLEFHKELLSREANGSF 307
             + L+ PL IP GV +      R               VHD +    K     E +   
Sbjct: 248 IALFLLAPLAIPIGVGFKELMSSRKTQQK----------VHDLEAPVDKFYFVEEDH--- 307

Query: 308 GNGGSQLFSESASLIDGEIESKGCFPKLVEKDQLAMLG--EEHPSSRLVKRLDFWLYFTA 367
                                     K  E+ + A++G  EE   ++L K+LDFW+YF  
Sbjct: 308 -------------------------TKEEEEFEKAIIGVKEEVEWTQLWKKLDFWIYFGL 367

Query: 368 YICGGTIGLVYSNNIGQIAQSLGLSSRTNAIVTLYSSFSFFGRLLSAVPDYIRAKLYFAR 427
           Y+ G T+GLV++NN+GQIA+S G S+ T+++V L SSF FFGRLL ++ DY  ++  +  
Sbjct: 368 YLFGPTVGLVFTNNLGQIAESRG-STATSSLVALSSSFGFFGRLLPSLLDYFFSRNKYMP 427

Query: 428 TGWLSIA-LIPTPIAFFLLSASSSTVTVYIGTALIGLSSGFIFAAAVSITAELFGPNSLG 487
           +  +S+A  +   +A FLL    S + +YIGTA+IG+ SG + + +V++TAELFG    G
Sbjct: 428 SSPVSMAGSLVAMVASFLLLLIDSDIALYIGTAMIGIFSGALTSLSVTMTAELFGTKHFG 487

Query: 488 VNHNILITNIPIGSLLYGMFAAVVYDSQGWNSGHGEAVVCMGRRCYFLTFVFCGCISVVG 547
           VNHNI++ +IP+GS  +G+ AA +Y  +   + +G+   C G  C+  T VF G +  + 
Sbjct: 488 VNHNIVVGSIPLGSFSFGLLAAKIY--RDGAALYGDDGKCFGMHCFQTTLVFWGMLCSIA 496

Query: 548 LVSSVLLFLRTRHAY 555
            + + +L++R R  Y
Sbjct: 548 ALLAAVLYIRNRKFY 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E14.2e-16557.44Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU87.6e-0527.37Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Match NameE-valueIdentityDescription
A0A6J1ETH80.0e+00100.00protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JBB10.0e+0098.77protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483... [more]
A0A1S3BTD84.0e-29192.48protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493060 PE=4 ... [more]
A0A6J1C4X11.2e-29091.05protein NUCLEAR FUSION DEFECTIVE 4 OS=Momordica charantia OX=3673 GN=LOC11100850... [more]
A0A0A0L0014.8e-28991.96Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G05545... [more]
Match NameE-valueIdentityDescription
AT4G19450.19.9e-21067.48Major facilitator superfamily protein [more]
AT5G45275.11.6e-20767.36Major facilitator superfamily protein [more]
AT1G31470.13.0e-16657.44Major facilitator superfamily protein [more]
AT3G01630.12.2e-14552.46Major facilitator superfamily protein [more]
AT2G30300.19.3e-7535.50Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 7..255
e-value: 2.8E-79
score: 266.2
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 347..558
e-value: 1.8E-8
score: 36.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 340..552
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 2..565
NoneNo IPR availablePANTHERPTHR21576:SF114NODULIN-LIKE/MFS TRANSPORTERcoord: 2..565
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 352..570
score: 8.931659

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G005650.1CmoCh16G005650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity