CmoCh16G005380 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G005380
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pectinesterase
Location	Cmo_Chr16: 2628087 .. 2630329 (+)
RNA-Seq Expression	CmoCh16G005380
Synteny	CmoCh16G005380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TACCATGACTCATAATAACCTCCTCATTAACATTGCCCTCTCTTCTCTCGTTCCTCTTTTTCTCGCTTTGCTCCTCGTCGTCATTCCTACCCCTCCTTTCTTCTCCGTTCCCGATGACTCCCTTAGCTGCCACCACCACCACCATAAACCCTCGGCTGATGAAGAAGACCGAGCGAGTATCGGTTCCATTTGTGACACCTTCCCGACTGCCCTTCTCCCACCAGAAACTGTCAACACTTCTGTCTTATGCGTTGACCAAAATGGATGCTGCAACTTCACTACTGTACAGGCAGCTGTGGATGCTGTGTTGAATATGAGCTCCAAAAGAAGCTTAATATGGATCAACACTGGAATCTATTTGTAAACTCTTTATATATATACCACCTATTTTAAATTACAAAAATACCCTTTGACTTTGTTTTTGTTTTTGTTTTTGTTTTATAACTACCTTTATTCATAGACGATAAGACGGATTGACCATCGGTACATCGAACGTGATGTTTTGTTGTTTTTTTAGTGAGAAGGTGATGGTTCCGAGATCGAAACCGAACGTGACATTTCAAGGACAAGGCTACACAACCACGGCTATTGTTTGGAACGACACTGCTAATTCTTCGCATGGCACCTTTTACAGCGGCTCCGTCCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGGTAATTTCTAATTAATTTTATATTATAATTTAATAGATTCTTCATTTATGACATTTTTTATTAAAAAAAATTGGAAAGTAATAATTTTTTTTTAATCTTGATCCATCCCTAAATTAGCTGATGTGGGACTTTTATCATCCAACTCCTCCCTAAGAACAAAGTACGTCTCCCGTTAATCGAGACTCGACTCCTTTGAAATCTTAGTCATTTTTTACTGCCTTAGAAGAGGCTTGACTCCTTTTTTTGGAGCCCTTTGTTCGATATTTGAGGATTTACCAATTTATTGGCACGACTAAGTTTGGGGCATGACTCTGATACTATGTTAGACGAACACGACTCTCAACAATGGTATGATATTGTTTACTTTGAGCATAAACTTGGGGTTCCCAAAAGGCCTCACATCAATCGAGAGTGTATTCTTTGATTATAAATCCTTCTAGCGCCAACAAATTTTCATTTTAATAAAGAAATTCCCAAACCATAGAAAGCAAAATAAATAAACCCCAAATTCAGTACGAAAGATACTCCCCGCGAAATTATAATTTAACATTTTGGTGCCTTGGCAGAATGTGGCGCCAATTCCAGGGCCGGGCGTCATCGGAGCACAGGCGGTGGCGATTCGGATCGGCGGAGACCAGGCGGCGTTTTGGAACTGCGGATTCTTCGGCGCTCAAGACACGCTCCACGATGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGAAATGGAAGATCTTACTACGAGGTAAATCGGAGCCCTAGTTTTTTCGCCGATTTCGATTTGACGATAAATTTCTGAATCGCGATTATGAAAACCTTGCCGCATTGTAGAGTTGCCAGATGATTTCGATGGCGAATCCAGTTCCGCAAGGATCAAAGTTCGTGAACGGAGCCGTGACAGCGCACGGCAGAGCCTCCGCAGACGAGAACTCTGGATTTGCTTTTGTTAATTGCAGCATCGGTGGAACCGGCCGAGTGTGGCTCGGTCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGTCGGAGGGATGGAATGACTTTAATGACCCCGCAAGGGACCAGTACGTTTTCTTTTTTCTAATTACTAAATTACCAACGTCTTATCCACTAAATTTAAAACTATAATTATTATTTAAGGAATTTTAATAATGGAAAAAAATTGAAATTTGCAGGACGATATTCTACGGGGAGTACAACTGCAGTGGAGCAGGAGCCAACATGAGCGCAAGGGCTCCCTATGTTCAGAGGCTCAATGATACTCAAGTTTCCCCTTTCCTCAACCTCTCCTTCATTGACGCAGACCAATGGCTGCAACCCTATTAATTCTCTCTTTTTCTTTCATACATTCTCATTATTTTTTATTTATTAATAATAATCATTAATTATATTATAAATATATTATCCATCATCATTTCCTAAATTAATTGAGGTGGACCTCCCCTCGAACAAAGTACGAGACATGACTCTAATACCATGTTAAATGAACACGACTCTCC

mRNA sequence

TACCATGACTCATAATAACCTCCTCATTAACATTGCCCTCTCTTCTCTCGTTCCTCTTTTTCTCGCTTTGCTCCTCGTCGTCATTCCTACCCCTCCTTTCTTCTCCGTTCCCGATGACTCCCTTAGCTGCCACCACCACCACCATAAACCCTCGGCTGATGAAGAAGACCGAGCGAGTATCGGTTCCATTTGTGACACCTTCCCGACTGCCCTTCTCCCACCAGAAACTGTCAACACTTCTGTCTTATGCGTTGACCAAAATGGATGCTGCAACTTCACTACTGTACAGGCAGCTGTGGATGCTGTGTTGAATATGAGCTCCAAAAGAAGCTTAATATGGATCAACACTGGAATCTATTTTGAGAAGGTGATGGTTCCGAGATCGAAACCGAACGTGACATTTCAAGGACAAGGCTACACAACCACGGCTATTGTTTGGAACGACACTGCTAATTCTTCGCATGGCACCTTTTACAGCGGCTCCGTCCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGAATGTGGCGCCAATTCCAGGGCCGGGCGTCATCGGAGCACAGGCGGTGGCGATTCGGATCGGCGGAGACCAGGCGGCGTTTTGGAACTGCGGATTCTTCGGCGCTCAAGACACGCTCCACGATGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGAAATGGAAGATCTTACTACGAGAGTTGCCAGATGATTTCGATGGCGAATCCAGTTCCGCAAGGATCAAAGTTCGTGAACGGAGCCGTGACAGCGCACGGCAGAGCCTCCGCAGACGAGAACTCTGGATTTGCTTTTGTTAATTGCAGCATCGGTGGAACCGGCCGAGTGTGGCTCGGTCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGTCGGAGGGATGGAATGACTTTAATGACCCCGCAAGGGACCAGACGATATTCTACGGGGAGTACAACTGCAGTGGAGCAGGAGCCAACATGAGCGCAAGGGCTCCCTATGTTCAGAGGCTCAATGATACTCAAGTTTCCCCTTTCCTCAACCTCTCCTTCATTGACGCAGACCAATGGCTGCAACCCTATTAATTCTCTCTTTTTCTTTCATACATTCTCATTATTTTTTATTTATTAATAATAATCATTAATTATATTATAAATATATTATCCATCATCATTTCCTAAATTAATTGAGGTGGACCTCCCCTCGAACAAAGTACGAGACATGACTCTAATACCATGTTAAATGAACACGACTCTCC

Coding sequence (CDS)

ATGACTCATAATAACCTCCTCATTAACATTGCCCTCTCTTCTCTCGTTCCTCTTTTTCTCGCTTTGCTCCTCGTCGTCATTCCTACCCCTCCTTTCTTCTCCGTTCCCGATGACTCCCTTAGCTGCCACCACCACCACCATAAACCCTCGGCTGATGAAGAAGACCGAGCGAGTATCGGTTCCATTTGTGACACCTTCCCGACTGCCCTTCTCCCACCAGAAACTGTCAACACTTCTGTCTTATGCGTTGACCAAAATGGATGCTGCAACTTCACTACTGTACAGGCAGCTGTGGATGCTGTGTTGAATATGAGCTCCAAAAGAAGCTTAATATGGATCAACACTGGAATCTATTTTGAGAAGGTGATGGTTCCGAGATCGAAACCGAACGTGACATTTCAAGGACAAGGCTACACAACCACGGCTATTGTTTGGAACGACACTGCTAATTCTTCGCATGGCACCTTTTACAGCGGCTCCGTCCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATAAGCTTCATGAATGTGGCGCCAATTCCAGGGCCGGGCGTCATCGGAGCACAGGCGGTGGCGATTCGGATCGGCGGAGACCAGGCGGCGTTTTGGAACTGCGGATTCTTCGGCGCTCAAGACACGCTCCACGATGACCGCGGCCGCCATTACTTCAAGGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGAAATGGAAGATCTTACTACGAGAGTTGCCAGATGATTTCGATGGCGAATCCAGTTCCGCAAGGATCAAAGTTCGTGAACGGAGCCGTGACAGCGCACGGCAGAGCCTCCGCAGACGAGAACTCTGGATTTGCTTTTGTTAATTGCAGCATCGGTGGAACCGGCCGAGTGTGGCTCGGTCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGTCGGAGGGATGGAATGACTTTAATGACCCCGCAAGGGACCAGACGATATTCTACGGGGAGTACAACTGCAGTGGAGCAGGAGCCAACATGAGCGCAAGGGCTCCCTATGTTCAGAGGCTCAATGATACTCAAGTTTCCCCTTTCCTCAACCTCTCCTTCATTGACGCAGACCAATGGCTGCAACCCTATTAA

Protein sequence

MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFSVPDDSLSCHHHHHKPSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQPY

Homology

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BLAST of CmoCh16G005380 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-145
Identity = 241/337 (71.51%), Postives = 283/337 (83.98%), Query Frame = 0


Query: 43  HHHHHKPSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHH HKPS    D     SICD FP  + P +T  TS LCVD+NGCCNFTTVQ+AVDAV 
Sbjct: 57  HHHRHKPS----DTKRKVSICDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVG 116

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
           N S +R++IWIN+G+Y+EKV++P++KPN+T QGQG+  TAI WNDTA S++GTFY  +VQ
Sbjct: 117 NFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQ 176

Query: 163 VFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
           VF S F+AKNISFMNVAPIP PG +GAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRH
Sbjct: 177 VFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRH 236

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           YFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+
Sbjct: 237 YFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFS 296

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IA EGWN+FNDP+RD TIFYGEYNCS
Sbjct: 297 FVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCS 356

Query: 343 GAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQ 380
           G GA+MS RAPYVQ+LN+TQV+  +N SFID DQWLQ
Sbjct: 357 GPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

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BLAST of CmoCh16G005380 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 4.2e-92
Identity = 169/337 (50.15%), Postives = 229/337 (67.95%), Query Frame = 0


Query: 43  HHHHHKPSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHHHH               C+ + T+ L  +   + VL VD +GC NF+ VQ+A+D V 
Sbjct: 54  HHHHHHHHHHHYHHHEPIKCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVP 113

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
           ++SS ++LI +N+G Y EKV V  +K N+  QG+GY  T+I WNDTA S+  T  S S  
Sbjct: 114 DLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFV 173

Query: 163 VFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
           VF++NF A NISF N AP P PG   AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH
Sbjct: 174 VFAANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRH 233

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           +FK+C+IQGSIDFIFGNGRS Y+ C + S+A     G   V G++TA GR S DE SGF+
Sbjct: 234 FFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFS 293

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNC I G+G + LGRAW  ++ VVF+NT M+ II  EGWN++ D  +++T+ +GE+ C 
Sbjct: 294 FVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCY 353

Query: 343 GAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQ 380
           G GA+   R  + ++L D++ S F+++SFID D+WL+
Sbjct: 354 GPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

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BLAST of CmoCh16G005380 vs. ExPASy Swiss-Prot
Match: Q9ZQA4 (Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 7.4e-89
Identity = 154/301 (51.16%), Postives = 207/301 (68.77%), Query Frame = 0


Query: 78  TSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQG 137
           T VL V  NGC  F  VQ A+DA +  S  ++LI I+ GIY E+ +V  +K N+  QG G
Sbjct: 39  TMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLVVQGMG 98

Query: 138 YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIG 197
           Y+ T+I WN+T  SS+GTF S SV VF   F A NISF N AP P PG + AQAVA+++ 
Sbjct: 99  YSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAVALKVV 158

Query: 198 GDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVP 257
           GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS YE C + S+A    
Sbjct: 159 GDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSIA---- 218

Query: 258 QGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDII 317
              +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP++RV+F+ T M+ ++
Sbjct: 219 --KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTYMSRVV 278

Query: 318 ASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQW 377
           + +GWND  DP   +T++YGE+ C G GAN S R  Y + L+D + +PF N+SFID ++W
Sbjct: 279 SLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFIDGEEW 333

Query: 378 L 379
           L
Sbjct: 339 L 333

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BLAST of CmoCh16G005380 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 9.0e-79
Identity = 140/296 (47.30%), Postives = 197/296 (66.55%), Query Frame = 0


Query: 90  NFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTA 149
           +FT +Q A+D++  ++  R +I ++ G+Y EKV +P  K  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 150 NSSH------GTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAF 209
            +        GT+ S S  V S  F+AKNI+F N  P+P PG +G QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 210 WNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFV 269
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 270 NGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWN 329
            GAVTA GR+S  E++GF+FV C + GTG ++LGRAW PFSRVVFA T M +II   GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 330 DFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQ 380
           ++ DP+R+ T+FYG+Y C+GAGAN   R  + + L D +  PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

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BLAST of CmoCh16G005380 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.4e-71
Identity = 150/354 (42.37%), Postives = 209/354 (59.04%), Query Frame = 0


Query: 37  DDSLSCHHHHHK---PSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTT 96
           DD    HHHH K   PS  +                          V+ V  NG   F +
Sbjct: 40  DDQQIQHHHHRKWVGPSGHK--------------------------VITVSLNGHAQFRS 99

Query: 97  VQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTAN--S 156
           VQ AVD++   ++K   I I  G Y EKV+VP +KP +TF+G G   TAI W+D A+   
Sbjct: 100 VQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASDLG 159

Query: 157 SHG----TFYSGSVQVFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCG 216
           ++G    T+ + SV V+++ F A+NISF N AP P PG+ G QAVA RI GD+A F  CG
Sbjct: 160 ANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCG 219

Query: 217 FFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAV 276
           F+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C++ S+A      S+F  G++
Sbjct: 220 FYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA------SRF--GSI 279

Query: 277 TAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFND 336
            AHGR   +E +GFAFV C + GTG +++GRA   +SR+V+A T    ++A  GW+D++ 
Sbjct: 280 AAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDH 339

Query: 337 PA-RDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQP 381
            + + +T F+G YNC G GA  +    + + L+     PF+  SF++   W+ P
Sbjct: 340 KSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAP 359

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BLAST of CmoCh16G005380 vs. ExPASy TrEMBL
Match: A0A6J1ETE2 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.4e-223
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0


Query: 1   MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFSVPDDSLSCHHHHHKPSADEEDRASIG 60
           MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFSVPDDSLSCHHHHHKPSADEEDRASIG
Sbjct: 1   MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFSVPDDSLSCHHHHHKPSADEEDRASIG 60

Query: 61  SICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFE 120
           SICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFE
Sbjct: 61  SICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFE 120

Query: 121 KVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAP 180
           KVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAP
Sbjct: 121 KVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAP 180

Query: 181 IPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNG 240
           IPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNG
Sbjct: 181 IPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNG 240

Query: 241 RSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAW 300
           RSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAW
Sbjct: 241 RSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAW 300

Query: 301 RPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLND 360
           RPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLND
Sbjct: 301 RPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLND 360

Query: 361 TQVSPFLNLSFIDADQWLQPY 382
           TQVSPFLNLSFIDADQWLQPY
Sbjct: 361 TQVSPFLNLSFIDADQWLQPY 381

[Close]

BLAST of CmoCh16G005380 vs. ExPASy TrEMBL
Match: A0A6J1J3X1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.3e-213
Identity = 368/382 (96.34%), Postives = 372/382 (97.38%), Query Frame = 0


Query: 1   MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFSVPDDSLSC-HHHHHKPSADEEDRASI 60
           MT NN+LINIALSSLVPLFLALLLVVIPTPPF SV DDSL+C HHHHHKPSADEED+ SI
Sbjct: 1   MTRNNVLINIALSSLVPLFLALLLVVIPTPPFLSVLDDSLNCHHHHHHKPSADEEDQTSI 60

Query: 61  GSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYF 120
           GSICDTFPTALLP ETV+TSVLCVD NGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYF
Sbjct: 61  GSICDTFPTALLPLETVDTSVLCVDLNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYF 120

Query: 121 EKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVA 180
           EKVMVPRSKPNVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVA
Sbjct: 121 EKVMVPRSKPNVTFQGQGYKTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVA 180

Query: 181 PIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN 240
           PIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN
Sbjct: 181 PIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN 240

Query: 241 GRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRA 300
           GRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRA
Sbjct: 241 GRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRA 300

Query: 301 WRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLN 360
           WRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSG GANMSARAPYVQRLN
Sbjct: 301 WRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPYVQRLN 360

Query: 361 DTQVSPFLNLSFIDADQWLQPY 382
           DTQ SPFLNLSFIDADQWLQPY
Sbjct: 361 DTQASPFLNLSFIDADQWLQPY 382

[Close]

BLAST of CmoCh16G005380 vs. ExPASy TrEMBL
Match: A0A1S3BRY7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.4e-190
Identity = 325/385 (84.42%), Postives = 350/385 (90.91%), Query Frame = 0


Query: 3   HNNLLINIALSSLVPLFLALLLVVIPTPPFFS---VPDDSLSC---HHHHHKPSADEEDR 62
           +NN LIN+ LSSL+PL LA L ++ PTP + S   V  D + C   HHHHH    DE+D+
Sbjct: 8   YNNFLINLTLSSLLPL-LAFLFMINPTPSYLSNLVVFGDPVDCPHPHHHHHHEDPDEDDQ 67

Query: 63  ASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTG 122
           + I SICD FPT L+PPET NTSV CVDQNGCCNFTTVQAAVDAV N+S KR++IWIN G
Sbjct: 68  SVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVPNLSIKRNIIWINAG 127

Query: 123 IYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFM 182
           IY+EKVMVP+SKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFM
Sbjct: 128 IYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFM 187

Query: 183 NVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI 242
           NVAPIPGPG +GAQAVAIR+GGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI
Sbjct: 188 NVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI 247

Query: 243 FGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWL 302
           FGNGRS+YESCQ++SMANPVPQGSKFVNGAVTAHGRASA+ENSGF+FVNCSIGGTGRVWL
Sbjct: 248 FGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNCSIGGTGRVWL 307

Query: 303 GRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQ 362
           GRAWRPFSRVVFANTVMTDIIA EGWNDFNDP RDQTIFYGEYNCSGAGANMS+RAPYVQ
Sbjct: 308 GRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGANMSSRAPYVQ 367

Query: 363 RLNDTQVSPFLNLSFIDADQWLQPY 382
           RLNDTQVS FLNLSFIDADQWLQPY
Sbjct: 368 RLNDTQVSSFLNLSFIDADQWLQPY 391

[Close]

BLAST of CmoCh16G005380 vs. ExPASy TrEMBL
Match: A0A0A0KVA9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.5e-185
Identity = 319/388 (82.22%), Postives = 347/388 (89.43%), Query Frame = 0


Query: 1   MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFS---VPDDSLSC----HHHHHKPSADE 60
           M +N+ LIN+ LSSL+PL LA L ++ PTP F S   V  D + C    H HHH  + DE
Sbjct: 5   MNYNHFLINLTLSSLLPL-LAFLFMINPTPSFLSNLVVFGDPVDCPHHHHPHHHHENPDE 64

Query: 61  EDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWI 120
           +D++ I SICD FPTAL+P ET NTS+ CVDQN CCNFTT+QAAVDAV N+S KR++IWI
Sbjct: 65  DDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPNLSIKRNIIWI 124

Query: 121 NTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNI 180
           N GIY+EKVMVP++K NVTFQGQGYTTTAIVWNDTANSSHGTFYS SVQVFSSNFIAKN+
Sbjct: 125 NAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIAKNL 184

Query: 181 SFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSI 240
           SFMNVAPIP PG +GAQ VAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSI
Sbjct: 185 SFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSI 244

Query: 241 DFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGR 300
           DFIFGNGRS+YESCQ++SMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGTGR
Sbjct: 245 DFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGR 304

Query: 301 VWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAP 360
           VWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSGAGANMS+RAP
Sbjct: 305 VWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANMSSRAP 364

Query: 361 YVQRLNDTQVSPFLNLSFIDADQWLQPY 382
           YVQRLNDTQVS FLNLSFIDADQWLQPY
Sbjct: 365 YVQRLNDTQVSSFLNLSFIDADQWLQPY 391

[Close]

BLAST of CmoCh16G005380 vs. ExPASy TrEMBL
Match: A0A6J1C3U9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.6e-182
Identity = 322/394 (81.73%), Postives = 345/394 (87.56%), Query Frame = 0


Query: 1   MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFSVPD-----DSLSC-----HHHHHK-- 60
           M + N L+ IALSSL+PL LA LL+  P P F S  D     D ++C     HHHHH+  
Sbjct: 1   MNNLNNLMTIALSSLIPL-LAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHHRRH 60

Query: 61  -PSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSSK 120
              ADEED+A+  SICD FP +LLP ET  TSV CVD+NGCCNFTTVQAAVDAV N+S K
Sbjct: 61  ESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAVPNLSLK 120

Query: 121 RSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSN 180
           R+LIWIN+GIY+EKVMVPRSK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SVQVFSSN
Sbjct: 121 RNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSN 180

Query: 181 FIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDC 240
           FIAKNISFMNVAPIPGPG +GAQAVA+RIGGD AAFW+CGFFGAQDTLHDDRGRHYFKDC
Sbjct: 181 FIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDC 240

Query: 241 YIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300
           YIQGSIDFIFGNGRS+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS
Sbjct: 241 YIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS 300

Query: 301 IGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGAN 360
           + GTGRVWLGRAWRPFSRVVF NTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSGAGAN
Sbjct: 301 VAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGAN 360

Query: 361 MSARAPYVQRLNDTQVSPFLNLSFIDADQWLQPY 382
             ARAP+VQRLNDTQVSPFLNLSFIDADQWL PY
Sbjct: 361 TGARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

[Close]

BLAST of CmoCh16G005380 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 517.7 bits (1332), Expect = 7.9e-147
Identity = 241/337 (71.51%), Postives = 283/337 (83.98%), Query Frame = 0


Query: 43  HHHHHKPSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHH HKPS    D     SICD FP  + P +T  TS LCVD+NGCCNFTTVQ+AVDAV 
Sbjct: 57  HHHRHKPS----DTKRKVSICDDFPKNIPPLDTDTTSYLCVDKNGCCNFTTVQSAVDAVG 116

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
           N S +R++IWIN+G+Y+EKV++P++KPN+T QGQG+  TAI WNDTA S++GTFY  +VQ
Sbjct: 117 NFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQ 176

Query: 163 VFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
           VF S F+AKNISFMNVAPIP PG +GAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRH
Sbjct: 177 VFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRH 236

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           YFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+
Sbjct: 237 YFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFS 296

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IA EGWN+FNDP+RD TIFYGEYNCS
Sbjct: 297 FVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCS 356

Query: 343 GAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQ 380
           G GA+MS RAPYVQ+LN+TQV+  +N SFID DQWLQ
Sbjct: 357 GPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

[Close]

BLAST of CmoCh16G005380 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 3.0e-93
Identity = 169/337 (50.15%), Postives = 229/337 (67.95%), Query Frame = 0


Query: 43  HHHHHKPSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVL 102
           HHHHH               C+ + T+ L  +   + VL VD +GC NF+ VQ+A+D V 
Sbjct: 54  HHHHHHHHHHHYHHHEPIKCCEKW-TSRLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVP 113

Query: 103 NMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 162
           ++SS ++LI +N+G Y EKV V  +K N+  QG+GY  T+I WNDTA S+  T  S S  
Sbjct: 114 DLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFV 173

Query: 163 VFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRH 222
           VF++NF A NISF N AP P PG   AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH
Sbjct: 174 VFAANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRH 233

Query: 223 YFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFA 282
           +FK+C+IQGSIDFIFGNGRS Y+ C + S+A     G   V G++TA GR S DE SGF+
Sbjct: 234 FFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFS 293

Query: 283 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCS 342
           FVNC I G+G + LGRAW  ++ VVF+NT M+ II  EGWN++ D  +++T+ +GE+ C 
Sbjct: 294 FVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCY 353

Query: 343 GAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQ 380
           G GA+   R  + ++L D++ S F+++SFID D+WL+
Sbjct: 354 GPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

[Close]

BLAST of CmoCh16G005380 vs. TAIR 10
Match: AT2G36700.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 328.9 bits (842), Expect = 5.2e-90
Identity = 154/301 (51.16%), Postives = 207/301 (68.77%), Query Frame = 0


Query: 78  TSVLCVDQNGCCNFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQG 137
           T VL V  NGC  F  VQ A+DA +  S  ++LI I+ GIY E+ +V  +K N+  QG G
Sbjct: 39  TMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLVVQGMG 98

Query: 138 YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIG 197
           Y+ T+I WN+T  SS+GTF S SV VF   F A NISF N AP P PG + AQAVA+++ 
Sbjct: 99  YSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAVALKVV 158

Query: 198 GDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVP 257
           GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS YE C + S+A    
Sbjct: 159 GDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSIA---- 218

Query: 258 QGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDII 317
              +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP++RV+F+ T M+ ++
Sbjct: 219 --KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTYMSRVV 278

Query: 318 ASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQW 377
           + +GWND  DP   +T++YGE+ C G GAN S R  Y + L+D + +PF N+SFID ++W
Sbjct: 279 SLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFIDGEEW 333

Query: 378 L 379
           L
Sbjct: 339 L 333

[Close]

BLAST of CmoCh16G005380 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 6.4e-80
Identity = 140/296 (47.30%), Postives = 197/296 (66.55%), Query Frame = 0


Query: 90  NFTTVQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTA 149
           +FT +Q A+D++  ++  R +I ++ G+Y EKV +P  K  +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 150 NSSH------GTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAF 209
            +        GT+ S S  V S  F+AKNI+F N  P+P PG +G QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 210 WNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFV 269
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 270 NGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWN 329
            GAVTA GR+S  E++GF+FV C + GTG ++LGRAW PFSRVVFA T M +II   GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 330 DFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQ 380
           ++ DP+R+ T+FYG+Y C+GAGAN   R  + + L D +  PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of CmoCh16G005380 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.7e-72
Identity = 150/354 (42.37%), Postives = 209/354 (59.04%), Query Frame = 0


Query: 37  DDSLSCHHHHHK---PSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTT 96
           DD    HHHH K   PS  +                          V+ V  NG   F +
Sbjct: 40  DDQQIQHHHHRKWVGPSGHK--------------------------VITVSLNGHAQFRS 99

Query: 97  VQAAVDAVLNMSSKRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTAN--S 156
           VQ AVD++   ++K   I I  G Y EKV+VP +KP +TF+G G   TAI W+D A+   
Sbjct: 100 VQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRASDLG 159

Query: 157 SHG----TFYSGSVQVFSSNFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCG 216
           ++G    T+ + SV V+++ F A+NISF N AP P PG+ G QAVA RI GD+A F  CG
Sbjct: 160 ANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCG 219

Query: 217 FFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAV 276
           F+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C++ S+A      S+F  G++
Sbjct: 220 FYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA------SRF--GSI 279

Query: 277 TAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFND 336
            AHGR   +E +GFAFV C + GTG +++GRA   +SR+V+A T    ++A  GW+D++ 
Sbjct: 280 AAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDH 339

Query: 337 PA-RDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQP 381
            + + +T F+G YNC G GA  +    + + L+     PF+  SF++   W+ P
Sbjct: 340 KSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIAP 359

The following BLAST results are available for this feature:

BLAST of CmoCh16G005380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23038	1.1e-145	71.51	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q9ZQA3	4.2e-92	50.15	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q9ZQA4	7.4e-89	51.16	Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1	[more]
Q8VYZ3	9.0e-79	47.30	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q8LPF3	2.4e-71	42.37	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]

BLAST of CmoCh16G005380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ETE2	1.4e-223	100.00	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1	[more]
A0A6J1J3X1	1.3e-213	96.34	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1	[more]
A0A1S3BRY7	3.4e-190	84.42	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1	[more]
A0A0A0KVA9	1.5e-185	82.22	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1	[more]
A0A6J1C3U9	1.6e-182	81.73	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1	[more]

BLAST of CmoCh16G005380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05310.1	7.9e-147	71.51	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	3.0e-93	50.15	Pectin lyase-like superfamily protein	[more]
AT2G36700.1	5.2e-90	51.16	Pectin lyase-like superfamily protein	[more]
AT5G19730.1	6.4e-80	47.30	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	1.7e-72	42.37	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 82..374 e-value: 1.6E-68 score: 230.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 79..381 e-value: 4.1E-99 score: 333.8
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 41..381
None	No IPR available	PANTHER	PTHR31321:SF33	PECTINESTERASE 8-RELATED	coord: 41..381
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 229..238
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 79..380

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh16G005380.1	CmoCh16G005380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity