CmoCh16G004950 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G004950
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description9-cis-epoxycarotenoid dioxygenase
LocationCmo_Chr16: 2388840 .. 2390824 (+)
RNA-Seq ExpressionCmoCh16G004950
SyntenyCmoCh16G004950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCCCAGACTGCATTTCGGTCTCCGGTTAAGACCACGGTGCCGAAACCGGTACCGGTTTCGGCTCCGTCAGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGCGGAGAAGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGTCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGTGTATTCGGGGGGTTTATGTTCGAAACGGCGCGAACCCACTTCATGAACCGGTGTCCGGTCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGGCGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCGAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTTACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAAAAGTCACCGGACGTTGAAATTCCCCTGCCTCAGCCAACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCATAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGTTCTCCGGTGATTTATGACAAAGAAAAAACCTCCCGATTTGGAGTTATGGACAAAAATGCCACCGACGCCGACGCCATTAAATGGATCGACGTTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACAGAGGAAATCGTCGTAATTGGGTCGTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAACTCCATCTTATCGGAAATTCGTCTGAATCTCTCCACCGGTAAATCGACTCGTCGGCCGATCATCGCCGAAACAGAGCAAGTGAACTTAGAGGCCGGAATGGTGAACCGAAATCAACTAGGGAGAAAAACCCAGTTCGTTTATGTTGCTCTAGCAGAGCCATGGCCGAAAGTTTCCGGTTTTGTGAAGGTCAATCTCTTCACCGGAGAATCCAGAAAGTATCTCTACGGCGAGCAGAGGTACGGCGGCGAGCCTCTGTTTCTGCCTAGAGAAGGTGCAGAGGCGGAGGACGACGGCCACATCTTGACGTTCGTTCACGACGAGAAGGAATGGAAATCAGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTGAAGCTTCCTTCTCGAGTTCCTTATGGCTTCCATGGAACATTCATAAGCTCCAATGATTTACAGAAGCAGAAACAGAAAAAAGACGAGGTAAATTATAACAACACACGGTAAATAAAACAAAAAAAGGGAAAAAAATTGGAGATTTTTACCTGTGGGATGAGTGTGCTTCTACGTCCCCGGAATCTCCTCTGTTTTCCTCTGTTTCATCTCTACAGCCATAGGTGTATAAAGTATAAATAAGGACTTTGTTGGGGGTGATAATGCAAATAGAATTAAATATGAGGGGCTTTAAGAAACCAGGCTCGAAGCTTTTGTGAAGCTCAGCTGGTTTCTGTTTATTCTTTCAGTCATTTGTGAATTATTAA

mRNA sequence

ATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCCCAGACTGCATTTCGGTCTCCGGTTAAGACCACGGTGCCGAAACCGGTACCGGTTTCGGCTCCGTCAGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGCGGAGAAGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGTCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGTGTATTCGGGGGGTTTATGTTCGAAACGGCGCGAACCCACTTCATGAACCGGTGTCCGGTCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGGCGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCGAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTTACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAAAAGTCACCGGACGTTGAAATTCCCCTGCCTCAGCCAACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCATAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGTTCTCCGGTGATTTATGACAAAGAAAAAACCTCCCGATTTGGAGTTATGGACAAAAATGCCACCGACGCCGACGCCATTAAATGGATCGACGTTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACAGAGGAAATCGTCGTAATTGGGTCGTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAACTCCATCTTATCGGAAATTCGTCTGAATCTCTCCACCGGTAAATCGACTCGTCGGCCGATCATCGCCGAAACAGAGCAAGTGAACTTAGAGGCCGGAATGGTGAACCGAAATCAACTAGGGAGAAAAACCCAGTTCGTTTATGTTGCTCTAGCAGAGCCATGGCCGAAAGTTTCCGGTTTTGTGAAGGTCAATCTCTTCACCGGAGAATCCAGAAAGTATCTCTACGGCGAGCAGAGGTACGGCGGCGAGCCTCTGTTTCTGCCTAGAGAAGGTGCAGAGGCGGAGGACGACGGCCACATCTTGACGTTCGTTCACGACGAGAAGGAATGGAAATCAGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTGAAGCTTCCTTCTCGAGTTCCTTATGGCTTCCATGGAACATTCATAAGCTCCAATGATTTACAGAAGCAGAAACAGAAAAAAGACGAGTCATTTGTGAATTATTAA

Coding sequence (CDS)

ATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCCCAGACTGCATTTCGGTCTCCGGTTAAGACCACGGTGCCGAAACCGGTACCGGTTTCGGCTCCGTCAGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGCGGAGAAGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGTCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGTGTATTCGGGGGGTTTATGTTCGAAACGGCGCGAACCCACTTCATGAACCGGTGTCCGGTCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGGCGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCGAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTTACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAAAAGTCACCGGACGTTGAAATTCCCCTGCCTCAGCCAACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCATAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGTTCTCCGGTGATTTATGACAAAGAAAAAACCTCCCGATTTGGAGTTATGGACAAAAATGCCACCGACGCCGACGCCATTAAATGGATCGACGTTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACAGAGGAAATCGTCGTAATTGGGTCGTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAACTCCATCTTATCGGAAATTCGTCTGAATCTCTCCACCGGTAAATCGACTCGTCGGCCGATCATCGCCGAAACAGAGCAAGTGAACTTAGAGGCCGGAATGGTGAACCGAAATCAACTAGGGAGAAAAACCCAGTTCGTTTATGTTGCTCTAGCAGAGCCATGGCCGAAAGTTTCCGGTTTTGTGAAGGTCAATCTCTTCACCGGAGAATCCAGAAAGTATCTCTACGGCGAGCAGAGGTACGGCGGCGAGCCTCTGTTTCTGCCTAGAGAAGGTGCAGAGGCGGAGGACGACGGCCACATCTTGACGTTCGTTCACGACGAGAAGGAATGGAAATCAGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTGAAGCTTCCTTCTCGAGTTCCTTATGGCTTCCATGGAACATTCATAAGCTCCAATGATTTACAGAAGCAGAAACAGAAAAAAGACGAGTCATTTGTGAATTATTAA

Protein sequence

MDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQKQKKDESFVNY
Homology
BLAST of CmoCh16G004950 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 3.0e-251
Identity = 420/574 (73.17%), Postives = 480/574 (83.62%), Query Frame = 0

Query: 3   SGRNRVACS---LHTPSIIQIPKRSQTAFRSPVK------TTVP----KPVPVSAPSVSE 62
           S  N + CS   LH P   Q    S T   +P+K      TT P    KP+  +     +
Sbjct: 44  SNTNTITCSLQTLHYPKQYQPTSTSTTTTPTPIKPTTTTTTTTPHRETKPLSDTKQPFPQ 103

Query: 63  EWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGK 122
           +WNFLQ+AAA  LD VE ALVS E KH LPKTADP VQIAGNFAPVPE +  + LPV+GK
Sbjct: 104 KWNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQALPVVGK 163

Query: 123 VPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQER 182
           +P+CI GVYVRNGANPL+EPV+GHH FDGDGMVHAV+FT +G  SYACRFTETQRL QE+
Sbjct: 164 IPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFT-NGAASYACRFTETQRLAQEK 223

Query: 183 AYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSE 242
           + GRPVFPKAIGELHGHSGIARLLLFYAR LF LVD SHG+GVANAGLVYFN  LLAMSE
Sbjct: 224 SLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMSE 283

Query: 243 DDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYF 302
           DDLPY +R+T  GDL TVGR+DF+GQL STMIAHPKLDPV+G++ ALSYDV+QKPYLKYF
Sbjct: 284 DDLPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKPYLKYF 343

Query: 303 KFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKT 362
           +FS +G KSPDVEIPL +PTMMHDFAITE FV++PDQQVVFKL EMI GGSPV+YDK KT
Sbjct: 344 RFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVYDKNKT 403

Query: 363 SRFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEE 422
           SRFG++DKNA DA+A++WID P+CFCFHLWNAWEEPET+EIVVIGSCMTP DSIFNEC+E
Sbjct: 404 SRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIFNECDE 463

Query: 423 NLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKV 482
           +L S+LSEIRLNL TGKSTRRPII++ EQVNLEAGMVNRN+LGRKTQF Y+ALAEPWPKV
Sbjct: 464 SLKSVLSEIRLNLRTGKSTRRPIISDAEQVNLEAGMVNRNKLGRKTQFAYLALAEPWPKV 523

Query: 483 SGFVKVNLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIV 542
           SGF KV+LF+GE +KY+YGE+++GGEPLFLP    E E DG+IL FVHDEKEWKSELQIV
Sbjct: 524 SGFAKVDLFSGEVQKYMYGEEKFGGEPLFLP--NGEEEGDGYILAFVHDEKEWKSELQIV 583

Query: 543 NAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 564
           NA  L+LEA++KLPSRVPYGFHGTFI S DL+KQ
Sbjct: 584 NAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQ 614

BLAST of CmoCh16G004950 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 867.5 bits (2240), Expect = 8.7e-251
Identity = 416/558 (74.55%), Postives = 476/558 (85.30%), Query Frame = 0

Query: 11  SLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPV---SAPSVSEEWNFLQRAAAMALDAVED 70
           SL  P I+  PK+S   +++P   T+  P      S+ S + +WN +Q+AAAMALDAVE 
Sbjct: 49  SLQAPPILHFPKQSSN-YQTPKNNTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVES 108

Query: 71  ALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLH 130
           AL   E +H LPKTADP VQI+GNFAPVPE  V + LPV GK+P+C++GVYVRNGANPL 
Sbjct: 109 ALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLF 168

Query: 131 EPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHS 190
           EP +GHH FDGDGMVHAV+F ++G  SYACRFTET+RLVQE+A GRPVFPKAIGELHGHS
Sbjct: 169 EPTAGHHFFDGDGMVHAVQF-KNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHS 228

Query: 191 GIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTV 250
           GIARL+LFYARGLFGLVDHS G GVANAGLVYFN +LLAMSEDDLPY ++VTP GDLKT 
Sbjct: 229 GIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTE 288

Query: 251 GRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQ 310
           GRFDFDGQLKSTMIAHPKLDPVSGE+FALSYDVIQKPYLKYF+FS  GEKS DVEIP+  
Sbjct: 289 GRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVED 348

Query: 311 PTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDADAIKW 370
           PTMMHDFAITE FV+IPDQQVVFK+ EMIRGGSPV+YDK K SRFG++DK A D   +KW
Sbjct: 349 PTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLKW 408

Query: 371 IDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKS 430
           ++VPDCFCFHLWNAWEE ET+EIVVIGSCMTPPDSIFNEC+E L S+LSEIRLNL TGKS
Sbjct: 409 VEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKS 468

Query: 431 TRRPIIAE-TEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTGESRKYL 490
           TR+ II    EQVNLEAGMVNRN+LGRKT++ Y+A+AEPWPKVSGF KVNLFTGE  K++
Sbjct: 469 TRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFI 528

Query: 491 YGEQRYGGEPLFLPRE-GAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSR 550
           YG+ +YGGEPLFLPR+  ++ EDDG+IL FVHDEKEWKSELQIVNAM+L+LEATVKLPSR
Sbjct: 529 YGDNKYGGEPLFLPRDPNSKEEDDGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPSR 588

Query: 551 VPYGFHGTFISSNDLQKQ 564
           VPYGFHGTFI++NDL  Q
Sbjct: 589 VPYGFHGTFINANDLANQ 604

BLAST of CmoCh16G004950 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 2.8e-249
Identity = 417/557 (74.87%), Postives = 475/557 (85.28%), Query Frame = 0

Query: 8   VACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVED 67
           V+ +LHTP  +  PK+S     SP     PK    +  S +++ N  QRAAA ALDA E 
Sbjct: 50  VSSALHTPPALHFPKQSS---NSPAIVVKPK----AKESNTKQMNLFQRAAAAALDAAEG 109

Query: 68  ALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLH 127
            LVS EK H LPKTADPSVQIAGNFAPV EQ VR+ LPV+GK+P+ I+GVYVRNGANPLH
Sbjct: 110 FLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLH 169

Query: 128 EPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHS 187
           EPV+GHH FDGDGMVHAV+F E G  SYACRFT+T R VQER  GRPVFPKAIGELHGH+
Sbjct: 170 EPVTGHHFFDGDGMVHAVKF-EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT 229

Query: 188 GIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTV 247
           GIARL+LFYAR   G+VD +HG GVANAGLVYFNG+LLAMSEDDLPY++++TP GDLKTV
Sbjct: 230 GIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTV 289

Query: 248 GRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQ 307
           GRFDFDGQL+STMIAHPK+DP SGE+FALSYDV+ KPYLKYF+FSP+G KSPDVEI L Q
Sbjct: 290 GRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQ 349

Query: 308 PTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDADAIKW 367
           PTMMHDFAITE FV++PDQQVVFKLPEMIRGGSPV+YDK K +RFG++DK A D+  IKW
Sbjct: 350 PTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKW 409

Query: 368 IDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKS 427
           ID PDCFCFHLWNAWEEPET+E+VVIGSCMTPPDSIFNE +ENL S+LSEIRLNL TG+S
Sbjct: 410 IDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGES 469

Query: 428 TRRPIIA-ETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTGESRKYL 487
           TRRPII+ E +QVNLEAGMVNRN LGRKT+F Y+ALAEPWPKVSGF KV+L TGE +K+L
Sbjct: 470 TRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHL 529

Query: 488 YGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRV 547
           YG+ RYGGEPLFLP EG E ED+G+IL FVHDEK WKSELQIVNA++LE+EATVKLPSRV
Sbjct: 530 YGDNRYGGEPLFLPGEGGE-EDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRV 589

Query: 548 PYGFHGTFISSNDLQKQ 564
           PYGFHGTFI ++DL KQ
Sbjct: 590 PYGFHGTFIGADDLAKQ 597

BLAST of CmoCh16G004950 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 5.4e-245
Identity = 406/534 (76.03%), Postives = 462/534 (86.52%), Query Frame = 0

Query: 31  PVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAG 90
           P +   PK  P    S    WNF Q+AAA ALD VE ALVS E ++ LPKTADP VQIAG
Sbjct: 51  PKQNNTPKNQPQFQTS---HWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAG 110

Query: 91  NFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTED 150
           NFAPVPEQSVR  LPV G +P+CI GVYVRNGANPL EPV+GHHLFDGDGMVHAV   E+
Sbjct: 111 NFAPVPEQSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVT-VEN 170

Query: 151 GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGI 210
           G VSY+CRFTET+RLVQER  G PVFPKAIGELHGHSGIARLLLFYARG+FGLVDHSHG 
Sbjct: 171 GSVSYSCRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGT 230

Query: 211 GVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVS 270
           GVANAGLV+FN +LLAMSEDD+PY ++V P+GDL+TVGR++FD QLKSTMIAHPK+DPVS
Sbjct: 231 GVANAGLVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVS 290

Query: 271 GEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVF 330
           GE+FALSYDV+QKPYLK FKFSP+GEKSPDVEIPL  PTMMHDFAITE +V+IPDQQVVF
Sbjct: 291 GELFALSYDVVQKPYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVF 350

Query: 331 KLPEMIRGGSPVIYDKEKTSRFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEI 390
           KL EMI+GGSPVIYDK K SRFG++ KNA +++ I W++  + FCFHLWNAWEEPET+E+
Sbjct: 351 KLQEMIKGGSPVIYDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEV 410

Query: 391 VVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQ 450
           +VIGSCMTPPDSIFNEC ENL S+LSEIRLNL TG+STRR +++ ++QVNLEAGMVNRN+
Sbjct: 411 IVIGSCMTPPDSIFNECNENLKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNK 470

Query: 451 LGRKTQFVYVALAEPWPKVSGFVKVNLFTGESRKYLYGEQRYGGEPLFLPRE-GAEAEDD 510
           LGRKTQF Y+A+AEPWPKVSGF KV+L TGE +K++YG++RYGGEPLFLPR   +E EDD
Sbjct: 471 LGRKTQFAYLAIAEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDD 530

Query: 511 GHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 564
           G+IL F HDEK WKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS DLQ Q
Sbjct: 531 GYILAFCHDEKTWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

BLAST of CmoCh16G004950 vs. ExPASy Swiss-Prot
Match: Q9M9F5 (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED9 PE=2 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 2.8e-233
Identity = 386/513 (75.24%), Postives = 448/513 (87.33%), Query Frame = 0

Query: 52  NFLQRAAAMALDAVEDALVSAE-KKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKV 111
           N +QR AAM LDAVE+A++S E ++H  PKTADP+VQIAGNF PVPE+ V   LPV G V
Sbjct: 146 NPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTV 205

Query: 112 PECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERA 171
           PECI+GVYVRNGANPLH+PVSGHHLFDGDGMVHAV F ++G VSYACRFTET RLVQER 
Sbjct: 206 PECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSVSYACRFTETNRLVQERE 265

Query: 172 YGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSED 231
            GRPVFPKAIGELHGH GIA+L+LF  RGLFGLVD + G+GVANAGLVYFNG LLAMSED
Sbjct: 266 CGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSED 325

Query: 232 DLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFK 291
           DLPY ++VT  GDL+T GR+DFDGQLKSTMIAHPK+DP + E+FALSYDV+ KPYLKYF+
Sbjct: 326 DLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFR 385

Query: 292 FSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTS 351
           F+ +GEKSPDVEIPL QPTM+HDFAITE FV+IPDQQVVF+LPEMIRGGSPV+YD++K S
Sbjct: 386 FTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKS 445

Query: 352 RFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEEN 411
           RFG+++KNA DA +I+WI+VPDCFCFHLWN+WEEPET+E+VVIGSCMTPPDSIFNE +E 
Sbjct: 446 RFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHDET 505

Query: 412 LNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVS 471
           L S+LSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRN LGRKT++ Y+AL EPWPKVS
Sbjct: 506 LQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVS 565

Query: 472 GFVKVNLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVN 531
           GF KV+L TGE RKY+YGE +YGGEPLFLP  G   ED G+I+ FVHDE++ KSELQ++N
Sbjct: 566 GFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVHDEEKVKSELQLIN 625

Query: 532 AMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 564
           A+ ++LEATV LPSRVPYGFHGTFIS  DL KQ
Sbjct: 626 AVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of CmoCh16G004950 vs. ExPASy TrEMBL
Match: A0A6J1ETU1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111437497 PE=3 SV=1)

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 572/574 (99.65%), Postives = 572/574 (99.65%), Query Frame = 0

Query: 1   MDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAM 60
           MDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAM
Sbjct: 29  MDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAM 88

Query: 61  ALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVR 120
           ALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVR
Sbjct: 89  ALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVR 148

Query: 121 NGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAI 180
           NGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAI
Sbjct: 149 NGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAI 208

Query: 181 GELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTP 240
           GELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTP
Sbjct: 209 GELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTP 268

Query: 241 AGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPD 300
           AGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPD
Sbjct: 269 AGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPD 328

Query: 301 VEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNAT 360
           VEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNAT
Sbjct: 329 VEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNAT 388

Query: 361 DADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRL 420
           DADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRL
Sbjct: 389 DADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRL 448

Query: 421 NLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTG 480
           NLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTG
Sbjct: 449 NLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTG 508

Query: 481 ESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATV 540
           ESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATV
Sbjct: 509 ESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATV 568

Query: 541 KLPSRVPYGFHGTFISSNDLQKQKQKKDESFVNY 575
           KLPSRVPYGFHGTFISSNDLQKQKQKKDE  VNY
Sbjct: 569 KLPSRVPYGFHGTFISSNDLQKQKQKKDE--VNY 600

BLAST of CmoCh16G004950 vs. ExPASy TrEMBL
Match: A0A6J1JCI7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111483246 PE=3 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 554/565 (98.05%), Postives = 558/565 (98.76%), Query Frame = 0

Query: 1   MDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAM 60
           MDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPV APSVSEEWNFLQRAAAM
Sbjct: 30  MDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVPAPSVSEEWNFLQRAAAM 89

Query: 61  ALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVR 120
           ALDAVEDALVS E+KHSLPKTADPSVQIAGNFAPVPEQ VRKGLPVIGKVPE IRG+YVR
Sbjct: 90  ALDAVEDALVSVERKHSLPKTADPSVQIAGNFAPVPEQPVRKGLPVIGKVPESIRGIYVR 149

Query: 121 NGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAI 180
           NGANPLHEPVSGHHLFDGDGMVHAVEFTEDG VSYACRFTETQRLVQERAYGRPVFPKAI
Sbjct: 150 NGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEVSYACRFTETQRLVQERAYGRPVFPKAI 209

Query: 181 GELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTP 240
           GELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTP
Sbjct: 210 GELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTP 269

Query: 241 AGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPD 300
           AGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPD
Sbjct: 270 AGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPD 329

Query: 301 VEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNAT 360
           VEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNAT
Sbjct: 330 VEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNAT 389

Query: 361 DADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRL 420
           DA AIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRL
Sbjct: 390 DATAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRL 449

Query: 421 NLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTG 480
           NLSTGKSTRRPIIAETEQVNLEAGMVNRN+LGRKTQF YVALAEPWPKVSGFVKV+LFTG
Sbjct: 450 NLSTGKSTRRPIIAETEQVNLEAGMVNRNRLGRKTQFAYVALAEPWPKVSGFVKVDLFTG 509

Query: 481 ESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATV 540
           ESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATV
Sbjct: 510 ESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATV 569

Query: 541 KLPSRVPYGFHGTFISSNDLQKQKQ 566
           KLPSRVPYGFHGTFISSNDLQKQKQ
Sbjct: 570 KLPSRVPYGFHGTFISSNDLQKQKQ 594

BLAST of CmoCh16G004950 vs. ExPASy TrEMBL
Match: A0A1L5JHV0 (9-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 523/562 (93.06%), Postives = 543/562 (96.62%), Query Frame = 0

Query: 5   RNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSV---SEEWNFLQRAAAMA 64
           +NRVACSLHTPSIIQIPK SQTAFRSPVKT VPKPVPVS PSV   SEEWNFLQRAAA+A
Sbjct: 33  QNRVACSLHTPSIIQIPKHSQTAFRSPVKTAVPKPVPVSPPSVSSGSEEWNFLQRAAAVA 92

Query: 65  LDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRN 124
           LDAVEDALVSAE+KHSLPKTADP+VQIAGNFAPVPEQ VRK LPV GKVP+CIRGVYVRN
Sbjct: 93  LDAVEDALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKALPVTGKVPDCIRGVYVRN 152

Query: 125 GANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIG 184
           GANPLHEPVSGHHLFDGDGMVHAVEF+E GGVSYACRFTETQRLVQERAYGRPVFPKAIG
Sbjct: 153 GANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKAIG 212

Query: 185 ELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPA 244
           ELHGHSGIARL+LFYARGLFGLVDHSHG+GVANAGLVYFNG+LLAMSEDDLPY+IRVTPA
Sbjct: 213 ELHGHSGIARLMLFYARGLFGLVDHSHGLGVANAGLVYFNGRLLAMSEDDLPYQIRVTPA 272

Query: 245 GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDV 304
           GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDV
Sbjct: 273 GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDV 332

Query: 305 EIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATD 364
           EIPLPQPTMMHDFAITEKFV+IPDQQVVFKLPEMIRGGSPV+YDKEKTSRFG++DKNATD
Sbjct: 333 EIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATD 392

Query: 365 ADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLN 424
           A+AIKWI+ PDCFCFHLWNAWEEPET+EIVVIGSCMTPPDSIFNECEENL S+LSEIRLN
Sbjct: 393 ANAIKWIEAPDCFCFHLWNAWEEPETDEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLN 452

Query: 425 LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTGE 484
           LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQF Y+ALAEPWPKVSGF KV+L TGE
Sbjct: 453 LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSGFAKVDLATGE 512

Query: 485 SRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVK 544
            RKYLYGEQRYGGEPLFLPREGA AEDDGHIL FVHDEKEWKSELQIVNAMTLELEATVK
Sbjct: 513 IRKYLYGEQRYGGEPLFLPREGATAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVK 572

Query: 545 LPSRVPYGFHGTFISSNDLQKQ 564
           LPSRVPYGFHGTFISS DLQKQ
Sbjct: 573 LPSRVPYGFHGTFISSKDLQKQ 594

BLAST of CmoCh16G004950 vs. ExPASy TrEMBL
Match: A0A6J1FPE2 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447037 PE=3 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 6.1e-308
Identity = 517/564 (91.67%), Postives = 539/564 (95.57%), Query Frame = 0

Query: 5   RNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVS---EEWNFLQRAAAMA 64
           RNRV+CSLHTPSIIQIPKRSQTAFRSP+K  VPKP PV+APSVS   +EWNFLQ+AAAMA
Sbjct: 20  RNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPKPEPVAAPSVSSPAKEWNFLQQAAAMA 79

Query: 65  LDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRN 124
           LDAVEDALVS EKKHSLPKTADPSVQIAGNFAPVPEQ VRK LPV GKVP+CIRGVYVRN
Sbjct: 80  LDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQPVRKVLPVTGKVPDCIRGVYVRN 139

Query: 125 GANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIG 184
           GANPLHEPVSGHHLFDGDGMVHAVEFTE G VSYACRFTET+RLVQERAYGRPVFPKAIG
Sbjct: 140 GANPLHEPVSGHHLFDGDGMVHAVEFTECGRVSYACRFTETERLVQERAYGRPVFPKAIG 199

Query: 185 ELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPA 244
           ELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNG+LLAMSEDDLPY+IRVTP+
Sbjct: 200 ELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPS 259

Query: 245 GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDV 304
           GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFS EGEKSPDV
Sbjct: 260 GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSAEGEKSPDV 319

Query: 305 EIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATD 364
           EIPLPQPTMMHDFAITEKFV+IPDQQVVFKLPEMIRGGSPV+YDKEKTSRFG++DKNATD
Sbjct: 320 EIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATD 379

Query: 365 ADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLN 424
           A+AIKWI+ PDCFCFHLWNAWEEPE+ EIVVIGSCMTPPDSIFNECEENL S+LSEIRLN
Sbjct: 380 ANAIKWIEAPDCFCFHLWNAWEEPESNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLN 439

Query: 425 LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTGE 484
           LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQF Y+ALAEPWPKVSGF KV+L TGE
Sbjct: 440 LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSGFAKVDLSTGE 499

Query: 485 SRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVK 544
            RKYLYG  RYGGEPLFLPREGA AEDDGHIL FVHDEKEWKSELQIVNAMTLELEATVK
Sbjct: 500 IRKYLYGADRYGGEPLFLPREGATAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVK 559

Query: 545 LPSRVPYGFHGTFISSNDLQKQKQ 566
           LPSRVPYGFHGTFISS DLQKQK+
Sbjct: 560 LPSRVPYGFHGTFISSKDLQKQKR 583

BLAST of CmoCh16G004950 vs. ExPASy TrEMBL
Match: A0A346DA07 (9-cis-epoxycarotenoid dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479551 PE=2 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 5.7e-306
Identity = 513/564 (90.96%), Postives = 538/564 (95.39%), Query Frame = 0

Query: 5   RNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVS---EEWNFLQRAAAMA 64
           RNR++CSLHTPSIIQIPKRSQTAFRSP+K  +PKP  V+APSVS   +EWNFLQ+AAA+A
Sbjct: 16  RNRLSCSLHTPSIIQIPKRSQTAFRSPIKIAMPKPELVAAPSVSSPTKEWNFLQQAAALA 75

Query: 65  LDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRN 124
           LDAVEDALVS E+KHSLPKTADPSVQIAGNFAPVPEQ VRK LPV GKVP+CIRGVYVRN
Sbjct: 76  LDAVEDALVSMERKHSLPKTADPSVQIAGNFAPVPEQPVRKVLPVTGKVPDCIRGVYVRN 135

Query: 125 GANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIG 184
           GANPLHEPVSGHHLFDGDGMVHAVEFTE G VSYACRFTET+RLVQERAYGRPVFPKAIG
Sbjct: 136 GANPLHEPVSGHHLFDGDGMVHAVEFTECGRVSYACRFTETERLVQERAYGRPVFPKAIG 195

Query: 185 ELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPA 244
           ELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNG+LLAMSEDDLPY+IRVTP+
Sbjct: 196 ELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPS 255

Query: 245 GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDV 304
           GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFS EGEKSPDV
Sbjct: 256 GDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSAEGEKSPDV 315

Query: 305 EIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATD 364
           EIPLPQPTMMHDFAITE FV+IPDQQVVFKLPEMIRGGSPV+YDKEKTSRFG++DKNATD
Sbjct: 316 EIPLPQPTMMHDFAITENFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATD 375

Query: 365 ADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLN 424
           A+AIKWI+ PDCFCFHLWNAWEEPET EIVVIGSCMTPPDSIFNECEENL S+LSEIRLN
Sbjct: 376 ANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLN 435

Query: 425 LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTGE 484
           LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQF Y+ALAEPWPKVSGF KV+L TGE
Sbjct: 436 LSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSGFAKVDLSTGE 495

Query: 485 SRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVK 544
            RKYLYGE RYGGEPLFLPREGA AEDDGHIL FVHDEKEWKSELQIVNAMTLELEATVK
Sbjct: 496 IRKYLYGEDRYGGEPLFLPREGATAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVK 555

Query: 545 LPSRVPYGFHGTFISSNDLQKQKQ 566
           LPSRVPYGFHGTFISS DLQKQK+
Sbjct: 556 LPSRVPYGFHGTFISSKDLQKQKR 579

BLAST of CmoCh16G004950 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 862.4 bits (2227), Expect = 2.0e-250
Identity = 417/557 (74.87%), Postives = 475/557 (85.28%), Query Frame = 0

Query: 8   VACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVSAPSVSEEWNFLQRAAAMALDAVED 67
           V+ +LHTP  +  PK+S     SP     PK    +  S +++ N  QRAAA ALDA E 
Sbjct: 50  VSSALHTPPALHFPKQSS---NSPAIVVKPK----AKESNTKQMNLFQRAAAAALDAAEG 109

Query: 68  ALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLH 127
            LVS EK H LPKTADPSVQIAGNFAPV EQ VR+ LPV+GK+P+ I+GVYVRNGANPLH
Sbjct: 110 FLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLH 169

Query: 128 EPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHS 187
           EPV+GHH FDGDGMVHAV+F E G  SYACRFT+T R VQER  GRPVFPKAIGELHGH+
Sbjct: 170 EPVTGHHFFDGDGMVHAVKF-EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT 229

Query: 188 GIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTV 247
           GIARL+LFYAR   G+VD +HG GVANAGLVYFNG+LLAMSEDDLPY++++TP GDLKTV
Sbjct: 230 GIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTV 289

Query: 248 GRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQ 307
           GRFDFDGQL+STMIAHPK+DP SGE+FALSYDV+ KPYLKYF+FSP+G KSPDVEI L Q
Sbjct: 290 GRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQ 349

Query: 308 PTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDADAIKW 367
           PTMMHDFAITE FV++PDQQVVFKLPEMIRGGSPV+YDK K +RFG++DK A D+  IKW
Sbjct: 350 PTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKW 409

Query: 368 IDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKS 427
           ID PDCFCFHLWNAWEEPET+E+VVIGSCMTPPDSIFNE +ENL S+LSEIRLNL TG+S
Sbjct: 410 IDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGES 469

Query: 428 TRRPIIA-ETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSGFVKVNLFTGESRKYL 487
           TRRPII+ E +QVNLEAGMVNRN LGRKT+F Y+ALAEPWPKVSGF KV+L TGE +K+L
Sbjct: 470 TRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHL 529

Query: 488 YGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRV 547
           YG+ RYGGEPLFLP EG E ED+G+IL FVHDEK WKSELQIVNA++LE+EATVKLPSRV
Sbjct: 530 YGDNRYGGEPLFLPGEGGE-EDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRV 589

Query: 548 PYGFHGTFISSNDLQKQ 564
           PYGFHGTFI ++DL KQ
Sbjct: 590 PYGFHGTFIGADDLAKQ 597

BLAST of CmoCh16G004950 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 809.3 bits (2089), Expect = 2.0e-234
Identity = 386/513 (75.24%), Postives = 448/513 (87.33%), Query Frame = 0

Query: 52  NFLQRAAAMALDAVEDALVSAE-KKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKV 111
           N +QR AAM LDAVE+A++S E ++H  PKTADP+VQIAGNF PVPE+ V   LPV G V
Sbjct: 146 NPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTV 205

Query: 112 PECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERA 171
           PECI+GVYVRNGANPLH+PVSGHHLFDGDGMVHAV F ++G VSYACRFTET RLVQER 
Sbjct: 206 PECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSVSYACRFTETNRLVQERE 265

Query: 172 YGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSED 231
            GRPVFPKAIGELHGH GIA+L+LF  RGLFGLVD + G+GVANAGLVYFNG LLAMSED
Sbjct: 266 CGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSED 325

Query: 232 DLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFK 291
           DLPY ++VT  GDL+T GR+DFDGQLKSTMIAHPK+DP + E+FALSYDV+ KPYLKYF+
Sbjct: 326 DLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFR 385

Query: 292 FSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTS 351
           F+ +GEKSPDVEIPL QPTM+HDFAITE FV+IPDQQVVF+LPEMIRGGSPV+YD++K S
Sbjct: 386 FTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKS 445

Query: 352 RFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEEN 411
           RFG+++KNA DA +I+WI+VPDCFCFHLWN+WEEPET+E+VVIGSCMTPPDSIFNE +E 
Sbjct: 446 RFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHDET 505

Query: 412 LNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVS 471
           L S+LSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRN LGRKT++ Y+AL EPWPKVS
Sbjct: 506 LQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVS 565

Query: 472 GFVKVNLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVN 531
           GF KV+L TGE RKY+YGE +YGGEPLFLP  G   ED G+I+ FVHDE++ KSELQ++N
Sbjct: 566 GFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVHDEEKVKSELQLIN 625

Query: 532 AMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 564
           A+ ++LEATV LPSRVPYGFHGTFIS  DL KQ
Sbjct: 626 AVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of CmoCh16G004950 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 793.9 bits (2049), Expect = 8.7e-230
Identity = 375/528 (71.02%), Postives = 442/528 (83.71%), Query Frame = 0

Query: 37  PKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVP 96
           P P P+     +  WN LQRAA+ ALD  E AL+  E+   LPKT DP  QI+GN+APVP
Sbjct: 62  PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVP 121

Query: 97  EQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYA 156
           EQSV+  L V GK+P+CI GVY+RNGANPL EPVSGHHLFDGDGMVHAV+ T +G  SY+
Sbjct: 122 EQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKIT-NGDASYS 181

Query: 157 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 216
           CRFTET+RLVQE+  G P+FPKAIGELHGHSGIARL+LFYARGLFGL++H +G GVANAG
Sbjct: 182 CRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAG 241

Query: 217 LVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 276
           LVYF+ +LLAMSEDDLPY++RVT  GDL+T+GRFDFDGQL S MIAHPK+DPV+ E+FAL
Sbjct: 242 LVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFAL 301

Query: 277 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMI 336
           SYDV++KPYLKYFKFSPEGEKSPDVEIPL  PTMMHDFAITE FV+IPDQQVVFKL +M 
Sbjct: 302 SYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMF 361

Query: 337 RGGSPVIYDKEKTSRFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSC 396
            G SPV YD EK SRFG++ +NA DA  + W++ P+ FCFHLWNAWE PET+E+VVIGSC
Sbjct: 362 LGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSC 421

Query: 397 MTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQ 456
           MTP DSIFNEC+E LNS+LSEIRLNL TGKSTRR II  + Q+NLEAGMVNRN LGRKT+
Sbjct: 422 MTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTR 481

Query: 457 FVYVALAEPWPKVSGFVKVNLFTGESRKYLYGEQRYGGEPLFLPRE-GAEAEDDGHILTF 516
           + Y+A+AEPWPKVSGF KV+L TGE + + YG ++YGGEP FLPR   ++ EDDG+I++F
Sbjct: 482 YAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSF 541

Query: 517 VHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 564
           VHDE+ W+SEL IVNA+TLELEATVKLPSRVPYGFHGTF++S D+  Q
Sbjct: 542 VHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of CmoCh16G004950 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 755.7 bits (1950), Expect = 2.6e-218
Identity = 357/515 (69.32%), Postives = 432/515 (83.88%), Query Frame = 0

Query: 52  NFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPVPEQSVRKGLPVIGKVP 111
           N  Q+AAA+A+DA E AL+S E+   LPKTADP VQIAGN++PVPE SVR+ L V G +P
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 112 ECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSYACRFTETQRLVQERAY 171
           +CI GVY+RNGANP+ EP +GHHLFDGDGMVHAV+ T +G  SYACRFT+T+RLVQE+  
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKIT-NGSASYACRFTKTERLVQEKRL 189

Query: 172 GRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDD 231
           GRPVFPKAIGELHGHSGIARL+LFYARGL GL+++ +G+GVANAGLVYFN +LLAMSEDD
Sbjct: 190 GRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDD 249

Query: 232 LPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKF 291
           LPY++++T  GDL+TVGR+DFDGQLKS MIAHPKLDPV+ E+ ALSYDV++KPYLKYF+F
Sbjct: 250 LPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRF 309

Query: 292 SPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSR 351
           SP+G KSP++EIPL  PTM+HDFAITE FV+IPDQQVVFKL EMI G SPV++D EK SR
Sbjct: 310 SPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSR 369

Query: 352 FGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENL 411
            G+M K+AT+A  I W++ P+ FCFHLWNAWE PETEEIVVIGSCM+P DSIFNE +E+L
Sbjct: 370 LGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESL 429

Query: 412 NSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFVYVALAEPWPKVSG 471
            S+LSEIR+NL T K+TRR ++   E VNLE GMVNRN+LGRKT+F ++A+A PWPKVSG
Sbjct: 430 RSVLSEIRINLRTRKTTRRSLLV-NEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSG 489

Query: 472 FVKVNLFTGESRKYLYGEQRYGGEPLFLP---REGAEAEDDGHILTFVHDEKEWKSELQI 531
           F KV+L TGE +KY+YG ++YGGEP FLP     G E EDDG+I   VHDE+   SELQI
Sbjct: 490 FAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQI 549

Query: 532 VNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 564
           +NA+ L+LEAT+KLPSRVPYGFHGTF+ SN+L  Q
Sbjct: 550 INAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

BLAST of CmoCh16G004950 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 642.5 bits (1656), Expect = 3.2e-184
Identity = 310/534 (58.05%), Postives = 400/534 (74.91%), Query Frame = 0

Query: 35  TVPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALV-SAEKKHSLPKTADPSVQIAGNFA 94
           T P P PV         N LQ+ AA  LD +E ++V   E+   LPK  DP+VQ++GNFA
Sbjct: 47  TSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFA 106

Query: 95  PVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGV 154
           PV E  V+ GL V+G++P C++GVY+RNGANP+  P++GHHLFDGDGM+HAV    D  V
Sbjct: 107 PVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQV 166

Query: 155 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVA 214
           SY+CR+T+T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GLVD + G+GVA
Sbjct: 167 SYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVA 226

Query: 215 NAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 274
           NAG+V+FNG+LLAMSEDDLPY++++   GDL+T+GRF FD Q+ S++IAHPK+D  +G++
Sbjct: 227 NAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDL 286

Query: 275 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLP 334
             LSY+V++KP+L+Y KF+  G+K+ DVEI LP+PTM+HDFAITE FV+IPDQQ+VFKL 
Sbjct: 287 HTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLS 346

Query: 335 EMIRGGSPVIYDKEKTSRFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEE---- 394
           EMIRGGSPVIY KEK +RFGV+ K       I W+DVPDCFCFHLWNAWEE  TEE    
Sbjct: 347 EMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPV 406

Query: 395 IVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRN 454
           IVVIGSCM+PPD+IF+E  E     LSEIRLN+ T +S R+ I+     VNLEAG +NR+
Sbjct: 407 IVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVT---GVNLEAGHINRS 466

Query: 455 QLGRKTQFVYVALAEPWPKVSGFVKVNLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDD 514
            +GRK+QFVY+A+A+PWPK SG  KV++  G   ++ YG  R+GGEP F+P EG   ED 
Sbjct: 467 YVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EGEGEEDK 526

Query: 515 GHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 564
           G+++ FV DE++ +SE  +V+A  ++  A V+LP RVPYGFHGTF+S N L++Q
Sbjct: 527 GYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M6E83.0e-25173.179-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
O240238.7e-25174.559-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
Q9LRR72.8e-24974.879-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
K4CJJ15.4e-24576.039-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
Q9M9F52.8e-23375.249-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1ETU10.0e+0099.659-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1JCI70.0e+0098.059-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A1L5JHV00.0e+0093.069-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1FPE26.1e-30891.679-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A346DA075.7e-30690.969-cis-epoxycarotenoid dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479551 PE... [more]
Match NameE-valueIdentityDescription
AT3G14440.12.0e-25074.87nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G78390.12.0e-23475.24nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT1G30100.18.7e-23071.02nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.12.6e-21869.32nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT3G24220.13.2e-18458.05nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 93..555
e-value: 6.4E-117
score: 391.6
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 41..563
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 41..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G004950.1CmoCh16G004950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
biological_process GO:0010162 seed dormancy process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0045549 9-cis-epoxycarotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen