CmoCh16G004890 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G004890
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptiongibberellin 2-beta-dioxygenase 1-like
LocationCmo_Chr16: 2353890 .. 2355617 (+)
RNA-Seq ExpressionCmoCh16G004890
SyntenyCmoCh16G004890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCCTCTGTTTCTTTAAAGGAGAAAGGAGAAAAAGGGAAACTTTCATTCATTCATTCTTGTTCTTGTTGTTGTTCTTGTTCTTCTCTCAAAACTTTCCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGCTTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCCGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGGTAACTAAAACACCCAAATCCCCATTTTCCCAGAATGCTCTGTTTTTCACTCTGTTTTTTTTTTTCTCTCTGTTCTCTGTTTCTACGTTTTAGCTCTGCTGTGAACGATTACATCTTGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAAAGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTACAATAAACACGACAACCTCTGTTCTTCATAATAAAAAAAAGATTCAAAATTTGAGGTTTTTCCCATCTGGGTTCTCCAAAAAAACATCACCCACCATGTTCTTCATTAAAATAATGAAACTTTTTGTGTCGATGAAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGAGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAATCTTTATCCAAAATTAGGGTTGGAGATGTTCGGGTTAGTAGTTTGGTTCGATATGGACGTGTAGAGATTCGAACTTTCGATCCCTAACTGTTAGCTTTTGGATAGTTTGGTTGAACTATAAGTAGAGATGACAGAATTCGAACATCTAATCTCTTAGTTTACTCATCAGCGTTGTTTTTGTTGTTAGTTTGTTATCCTTGTACACACAGTCTTCTTAATACAAAGGGTATTACTGTTTTTTCTTTTAGGGTATTATGAGTTGTCAAAAATAGTACAACACGTTGGTGTTTGTAAGAAACGTGGGAGTTCTCGAAAAGAAGGGTTGTTGTCTCGAGATGTCTATTTGTGAGAAACGAGGATGTGTAAGTAATAGCCA

mRNA sequence

TTCTTCCTCTGTTTCTTTAAAGGAGAAAGGAGAAAAAGGGAAACTTTCATTCATTCATTCTTGTTCTTGTTGTTGTTCTTGTTCTTCTCTCAAAACTTTCCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGCTTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCCGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTTGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAAAGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGAGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAATCTTTATCCAAAATTAGGGTTGGAGATGTTCGGGTTAGTAGTTTGGTTCGATATGGACGTGTAGAGATTCGAACTTTCGATCCCTAACTGTTAGCTTTTGGATAGTTTGGTTGAACTATAAGTAGAGATGACAGAATTCGAACATCTAATCTCTTAGTTTACTCATCAGCGTTGTTTTTGTTGTTAGTTTGTTATCCTTGTACACACAGTCTTCTTAATACAAAGGGTATTACTGTTTTTTCTTTTAGGGTATTATGAGTTGTCAAAAATAGTACAACACGTTGGTGTTTGTAAGAAACGTGGGAGTTCTCGAAAAGAAGGGTTGTTGTCTCGAGATGTCTATTTGTGAGAAACGAGGATGTGTAAGTAATAGCCA

Coding sequence (CDS)

ATGAGAAGCTCCACGTCCATGGCGGCTGCTTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCCGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTTGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAAAGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGAGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAA

Protein sequence

MRSSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVPFERIAAS
Homology
BLAST of CmoCh16G004890 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 4.1e-128
Identity = 227/311 (72.99%), Postives = 261/311 (83.92%), Query Frame = 0

Query: 18  FFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQ 77
           F S IPL+DLS PDAK LIVKACE+ GFFKV+NHG+P++ IS LESE+ KFFSLP +EK+
Sbjct: 23  FSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKE 82

Query: 78  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIL 137
           +AGP +PFGYGNK+IG NGD+GW+EYLLL T    N D   S++G+D  K R  + DY  
Sbjct: 83  KAGPANPFGYGNKRIGLNGDIGWIEYLLLTT----NQDHNFSLYGEDIDKFRGLLKDYKC 142

Query: 138 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGF 197
           A+RNMA EIL+LMAEGL IQ +NVFSKLVMD+QSD +FRVNHYP CP+L A+ G N+IGF
Sbjct: 143 AMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNHYPACPEL-AINGENLIGF 202

Query: 198 GEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKH 257
           GEHTDPQIIS+LRSNNTSGFQISL DG+WISVPPDHSSFFINVGDSLQVMTNGRFKSV+H
Sbjct: 203 GEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFINVGDSLQVMTNGRFKSVRH 262

Query: 258 RVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADN 317
           RVL N    R+SMIYF GPPLSEKIAPL SLM+G+E SLYKEFTWFEYK S Y SRLADN
Sbjct: 263 RVLANGIDPRLSMIYFCGPPLSEKIAPLPSLMKGKE-SLYKEFTWFEYKSSTYGSRLADN 322

Query: 318 RLVPFERIAAS 329
           RL  +ERIAA+
Sbjct: 323 RLGNYERIAAT 327

BLAST of CmoCh16G004890 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 7.0e-120
Identity = 208/313 (66.45%), Postives = 253/313 (80.83%), Query Frame = 0

Query: 15  SAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLS 74
           S   F+GIP++DL+ PDAK LIV AC + GFFK+VNHGVP+E +++LE+E+ +FF    S
Sbjct: 20  STPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQS 79

Query: 75  EKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVND 134
           EK RAGPP PFGYG+K+IG NGDVGWVEYLLLNT+ +  S   L IF ++P   R+ V +
Sbjct: 80  EKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVEN 139

Query: 135 YILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNM 194
           YI AV+NM   +LELMAEGL I+QRN  S+L+ DE+SDS FR+NHYPPCP++QAL   N+
Sbjct: 140 YITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL-NRNL 199

Query: 195 IGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKS 254
           +GFGEHTDPQIISVLRSN+TSG QI L DG W+SVPPD +SFFINVGD+LQVMTNGRFKS
Sbjct: 200 VGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNGRFKS 259

Query: 255 VKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRL 314
           VKHRVL +++KSR+SMIYFGGP LSE IAPL S+M   E  LYKEFTW EYK++AY SRL
Sbjct: 260 VKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAAYTSRL 319

Query: 315 ADNRLVPFERIAA 328
           ADNRL PF++ AA
Sbjct: 320 ADNRLAPFQKSAA 331

BLAST of CmoCh16G004890 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 389.4 bits (999), Expect = 4.0e-107
Identity = 193/309 (62.46%), Postives = 234/309 (75.73%), Query Frame = 0

Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEK-Q 78
           FS IP+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 79  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIL 138
            AG   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+ +Y  
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 139 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCP--DLQALKGTNMI 198
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 199 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 258
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 259 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 318
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 319 DNRLVPFER 325
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

BLAST of CmoCh16G004890 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 5.0e-102
Identity = 181/303 (59.74%), Postives = 234/303 (77.23%), Query Frame = 0

Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGP 81
           IP+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 82  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRN 141
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 201
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   + +A K    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE-EAEKMVK-VGFGEHT 210

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 322 FER 325
           FE+
Sbjct: 331 FEK 331

BLAST of CmoCh16G004890 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-99
Identity = 181/306 (59.15%), Postives = 224/306 (73.20%), Query Frame = 0

Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQR 78
           FSG+P++DL +P A + +V ACE  GFFKVVNHGV  + +   ESE+ +FFS    +K R
Sbjct: 25  FSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESEAVRFFSQTQPDKDR 84

Query: 79  AGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILA 138
           +GP  PFGYG+K+IG NGD+GW+EYLLL     S +D   +         R+A+N+YI  
Sbjct: 85  SGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLAD---ACTVPSCAVFRAALNEYISG 144

Query: 139 VRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFG 198
           VR +A  ++E M+EGL I Q +  S LV  E SD VFRVNHYPPC  LQ L G ++ GFG
Sbjct: 145 VRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFRVNHYPPCRALQGL-GCSVTGFG 204

Query: 199 EHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHR 258
           EHTDPQ++SVLRSN TSG QI+L DG W+SVP D  SFF+NVGDSLQV+TNGRFKSVKHR
Sbjct: 205 EHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSLQVLTNGRFKSVKHR 264

Query: 259 VLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNR 318
           V+ NS KSRVS IYFGGPPL+++IAPL  L+   E+SLYKEFTW EYK++AY SRL DNR
Sbjct: 265 VVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQSLYKEFTWDEYKKAAYKSRLGDNR 324

Query: 319 LVPFER 325
           L  FE+
Sbjct: 325 LAQFEK 326

BLAST of CmoCh16G004890 vs. ExPASy TrEMBL
Match: A0A6J1ETB7 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111437550 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.3e-182
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MRSSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS
Sbjct: 88  MRSSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 147

Query: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 148 LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 207

Query: 121 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 180
           FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY
Sbjct: 208 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 267

Query: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 268 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 327

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 328 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 387

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 329
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 388 TWFEYKRSAYNSRLADNRLVPFERIAAS 415

BLAST of CmoCh16G004890 vs. ExPASy TrEMBL
Match: A0A6J1JC86 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483149 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 4.9e-177
Identity = 321/328 (97.87%), Postives = 322/328 (98.17%), Query Frame = 0

Query: 1   MRSSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAAA SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISS
Sbjct: 1   MRSSTSMAAA-SSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISS 60

Query: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 120

Query: 121 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 180
           FGQDPQKLRSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHY
Sbjct: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHY 180

Query: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 329
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 327

BLAST of CmoCh16G004890 vs. ExPASy TrEMBL
Match: Q7X9N5 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 7.9e-175
Identity = 313/320 (97.81%), Postives = 315/320 (98.44%), Query Frame = 0

Query: 9   AASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           AA+SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLESESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 121

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

BLAST of CmoCh16G004890 vs. ExPASy TrEMBL
Match: Q8GUE8 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.7e-174
Identity = 312/320 (97.50%), Postives = 315/320 (98.44%), Query Frame = 0

Query: 9   AASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           AA+SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLESESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQKL 121

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

BLAST of CmoCh16G004890 vs. ExPASy TrEMBL
Match: A0A6J1FNB4 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447347 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 299/320 (93.44%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 9   AASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           AA+ SSSAAFFSG+PLIDLSAPDAK LIVKACEELGFFKVVNHGVPMEFIS+LESESTKF
Sbjct: 22  AAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKF 81

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 82  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 141

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVNDYI AVRNMA EILELMAEGL IQQRN FSKL+MDE+SDSVFRVNHYPPCP+LQ 
Sbjct: 142 RSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELQT 201

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKGTNMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD SSFFINVGDSLQVMT
Sbjct: 202 LKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMT 261

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTN  KSRVSMIYFGGPPLSEKIAPL SLM+GEE SLYKEFTWFEYKRS
Sbjct: 262 NGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKRS 321

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLV FERIAAS
Sbjct: 322 AYNSRLADNRLVHFERIAAS 340

BLAST of CmoCh16G004890 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 389.4 bits (999), Expect = 2.8e-108
Identity = 193/309 (62.46%), Postives = 234/309 (75.73%), Query Frame = 0

Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEK-Q 78
           FS IP+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 79  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIL 138
            AG   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+ +Y  
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 139 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCP--DLQALKGTNMI 198
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 199 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 258
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 259 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 318
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 319 DNRLVPFER 325
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

BLAST of CmoCh16G004890 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 372.5 bits (955), Expect = 3.6e-103
Identity = 181/303 (59.74%), Postives = 234/303 (77.23%), Query Frame = 0

Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGP 81
           IP+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 82  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRN 141
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 201
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   + +A K    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE-EAEKMVK-VGFGEHT 210

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 322 FER 325
           FE+
Sbjct: 331 FEK 331

BLAST of CmoCh16G004890 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 344.7 bits (883), Expect = 7.9e-95
Identity = 169/303 (55.78%), Postives = 218/303 (71.95%), Query Frame = 0

Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGP 81
           IP+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+  FF+L  S K +AGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 82  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRN 141
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV +YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 201
           M+ + LE++ E L I+ +   S+LV  ++SDS  R+NHY   P+ +       IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQ+IS+LRSN+T G QI + DG W+ V PDHSSFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           N+ +SR+SMIYF GPPLSEKIAPL+ L+  ++  LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 322 FER 325
           FE+
Sbjct: 327 FEK 326

BLAST of CmoCh16G004890 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 292.7 bits (748), Expect = 3.6e-79
Identity = 158/327 (48.32%), Postives = 209/327 (63.91%), Query Frame = 0

Query: 3   SSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQL---IVKACEELGFFKVVNHGVPMEFIS 62
           SST +      + ++     P+ID S  D  +L   IVKACE  GFFKV+NHGV  E I 
Sbjct: 5   SSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIK 64

Query: 63  SLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLS 122
             E E  +FF+ P S+K RAGP SPFGYG K IG NGD+G +EYLLL+ +  + +D   +
Sbjct: 65  RFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSET 124

Query: 123 IFGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNH 182
           I   DP K  SA NDYI  VR++A EI++L  E L  Q+ +  S+L+ D +SDS+ R+NH
Sbjct: 125 ISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNH 184

Query: 183 YPPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFIN 242
           YPP P   AL G   IGFGEH+DPQI++VLRSN+  G +I   DG WI +P D + FF+ 
Sbjct: 185 YPPAP--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVL 244

Query: 243 VGDSLQVMTNGRFKSVKHRVLTNSSKS-RVSMIYFGGPPLSEKIAPLASLMQGEERSLYK 302
           VGD LQ +TNGRF SV+HRVL N++K  R+S +YF  PPL  KI+PL  ++  E    Y 
Sbjct: 245 VGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYN 304

Query: 303 EFTWFEYKRSAYNSRLADNRLVPFERI 326
            FTW +YK++ Y+ RL   RL  F+ +
Sbjct: 305 SFTWGDYKKATYSLRLDVPRLEFFKTL 329

BLAST of CmoCh16G004890 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 276.2 bits (705), Expect = 3.5e-74
Identity = 163/323 (50.46%), Postives = 198/323 (61.30%), Query Frame = 0

Query: 12  SSSSAAFFSGIPLIDLS--APDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFF 71
           S    A    IP+ID+S         IVKACE LGFFKV+NHGV    IS +E ES  FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 72  SLPLSEKQRAGPPS-PFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 131
           + P  EK+   P + PF YG + IG NGD G VEYLL +T    N   F S         
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHT----NDPAFRS-----QLSF 124

Query: 132 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDL-- 191
            SAVN YI AV+ +A EIL+L AEGL +   + FS+L+    SDSV RVNHYPP      
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 192 ------QALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 251
                 Q++  T  +GFGEHTDPQI++VLRSN   G Q+S +DG W+SV PD S+F +NV
Sbjct: 185 EANLSDQSVSLTR-VGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNV 244

Query: 252 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPL-ASLMQGEERSLYKE 311
           GD LQVMTNGRF SV+HR LT   +SR+S  YF GPPL  KI PL A +M   +  LY+ 
Sbjct: 245 GDLLQVMTNGRFISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQT 304

Query: 312 FTWFEYKRSAYNSRLADNRLVPF 323
           FTW EYK+ AY+ RL D+RL  F
Sbjct: 305 FTWGEYKKRAYSLRLEDSRLDMF 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SQ804.1e-12872.99Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
Q9XG837.0e-12066.45Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Q8LEA24.0e-10762.46Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ... [more]
Q9XFR95.0e-10259.74Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ... [more]
Q8S0S61.4e-9959.15Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
Match NameE-valueIdentityDescription
A0A6J1ETB71.3e-182100.00gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111437... [more]
A0A6J1JC864.9e-17797.87gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148314... [more]
Q7X9N57.9e-17597.81Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1[more]
Q8GUE81.7e-17497.50Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1[more]
A0A6J1FNB41.1e-16593.44gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447... [more]
Match NameE-valueIdentityDescription
AT1G78440.12.8e-10862.46Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
AT1G30040.13.6e-10359.74gibberellin 2-oxidase [more]
AT2G34555.17.9e-9555.78gibberellin 2-oxidase 3 [more]
AT1G02400.13.6e-7948.32gibberellin 2-oxidase 6 [more]
AT1G47990.13.5e-7450.46gibberellin 2-oxidase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 36..53
score: 54.55
coord: 200..221
score: 27.69
coord: 236..262
score: 33.0
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 11..324
NoneNo IPR availablePANTHERPTHR47990:SF125GIBBERELLIN 2-OXIDASEcoord: 11..324
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 19..316
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 22..113
e-value: 4.6E-16
score: 59.6
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..316
e-value: 3.8E-79
score: 268.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 172..274
e-value: 6.9E-28
score: 97.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 170..276
score: 12.386265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G004890.1CmoCh16G004890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding