CmoCh16G004330 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G004330
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionEnolase-phosphatase E1
LocationCmo_Chr16: 2054272 .. 2058682 (-)
RNA-Seq ExpressionCmoCh16G004330
SyntenyCmoCh16G004330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCTCTAGCTTATGTGGACACCAAGGCTGCAAACCATACGAAGGCTCTGATGGCGGAGCTTTCCCGCCAATTCTACGGCCTCCGATTGGTTTCCGGCCCCGCCGGTTGCATTTCCATCAAGGTTCACGACCACTCCATTCCCAAGGGACAACACACAGTCGCTTTGTCGCATTCCGGTTGGAATTTCTCCATCTTTCTCTTTTTAATTCTTTTTGGCTGTTTGTAACTGGCGTATGAATATGGCTAGGCGGCGTTGTAATTCAAACTAGTTCTTGTTGGGAGGATATCCAATTTCCCCTTCTCTTTGCAGAAGAAGATGATCTTAATGGTTCACGCCATCAAGAACTAGCCTAAGATCACCTGTTTTTCCAAACATTCTTTATTTTTTGTTTGGCAAGAGGATCTTTTAAACAATTCAACTCCCTGCATAAGAGTATAAATGGCTTGGAGTTCACGGATTTAGATGAATTTTCTTTTGCTTTTGTTTGATTATACACAGGTGTTAAGAAGGAGCGAATGGTAGCGGAGGACACCTGCATGTTATCTTATGGTGGGTCAATCTTATATTCACCACCTCCAAAGCCATTTTCTCACAAGCTCCCGTACTGTTTGGATGGTGGACCTCTTTTTATGAAGGTACAGTTCCCTGTATAAGAGTATAATCAACTTGTGATGAATGGAAAATACTTAGTTATGCTATAAATGAGCTAATGTTCATGTAAGCAATGTCTCCTATACCTCGAGACATTAACTTCACTGATCTTTGCATGATCGACCAACCTTTTTCGTTCTTATTTGATTGCCAAATCCATCCACCACAACCAAATTTGTGTTTCTTATTAAACACGGCAACAATAACTATTGCTGTGGCATTACTTTAAGTTAGGAGCAGGTTTTGTTTTGTTATTTTGCGTCGTAAATCTCAAAATTATTCAGCATTGTATGTGCTTTTTCTTATCGTCCCGTTTTATTCGATAGAAACCTTCGAACGAAAAGTTCCTTTGAGCTTTGAAGATGATGAAACCTCTGATTTTATACAATTTTAAGAAATTGATTTCGAGTCTGTCTTGCTTTCTTTCGGTCTCAGTAATCAATAACTTAATTTTACAGGCTGATTGTTATCACTATCTCTTTGATGCTGCTATTAAGCTTCGTCAACTAGGCATTGATTAGTCTTCTCCAACTCAGGATCCTTTTGGGAGCATCAAAGGAATGTCAAGCAAAATTTCTGTGAAGGCTGGGGCCCAAGGTTTAGATAATGAAAACAAACCATTGCCAGTGAGTTGAAGTGCATTTTGTTTAATTGGGAAGCTTAAAATTTTAGCCTTTGATTTTTCATTCTTTTCTTTACCTTTGAGGTATATCATTTTTGTATCTGGGTTAGGAATCTTCTTTTCAATTGTGGTTCATTTCTCCTTTATATTTTGGATGCAGCGTTGCATAGTTCTTGATATCGAAGGGACTACTACACCAATATCTTTCGTTACTGACGTTCTCTTTCCATATGCTCGTGATAATGTTGGAAAGCATTTGATTTTAACGTATGAAACTGCAGAAACTCAAGATGACATTAAGTTATTGCGTTCACAAGTAAGATATTTTGGTTCAACAGTATTTTGATTATGTTGCAGTCTGTAGAATGTCTACGATCTATTTACGGTATTTACTTGGGCATTGTTAGTTAAACAGGAATAGCCTGTGCGTTCATGAGTTAATATGTGATACAGAAATTTCCTCCTAAATTCAATTTGTGTGAGATCTCACATCGGTTGGGGAGGAGAACGAAACACCCTTTACAAGGGCGTGAAAATTTCTCCTAGCAGACGTATTTTAAAAACCTTGAGGGGAAGCCCAAAAGGAAAAGTCCAAAAAGGACAATATTTGCTAGCGGTGGGCTTGGGTTGTTAGAAATAGAATCAGAGCCAGACACCAGGCGATGTGCCAGCGAGGAGGCTGAGCCCCGAAGGGGGGTGGACATGAGGTGATGTGCCAGCAAGGACGTTGGGCCTCGAAGGGGTAGATTTAGTGGGGGTCCCACATCGATTGGAGAATGGTATGAGTGCCAGTGAGGACGCTGGGCCCTGAAGGGAGGTGGATTGTGAGATCCCACATCGGTTAGGGAGGAGAACGAAACACACTTTATAAGGGTGTGGAAACCTCTCCTTAGCAGACACATTTTAAAAACCTTGAGGGGAAATCCAAAAGGGAAAGCCCAAAGAGGATAATATCTGTTAGCGGTGGGCTTGGGCTTGGGCAGTTAGCAGTTAGAATTTGGTACTTGAGAGGCTATTTGGGATTTGTTTTCAGAATCTTCTGTTCCTCAATACAAAGATTCAGCGAAAATATGTTTGGATAACCAAATTCCAAAAGTTCTAAATTTTGCAGATGAATCATTACGTCGTTGATGTGAGTGTTTATCAATAAAAATGTGTTTCAGGTTGAAGAAGATTTGGAAAAGGGTGTTGCTGGAGCTGTGCCCATACCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAAGCAATGATAAAAGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGTATGCAAGTAAAATGATTCTCAATTTATCTTACTGCAATTCATTTGGCAGATTTTCTGAGTGAGCTGACTTCTAGCTTTGCAGGGTCACATATGGAGAACAGGATTTGCAGGGAATGAATTGGAGGGAATAGTTTTTGATGATGTGCCAGAAGCTCTTGAGAGATGGCATGCTTTGGGCATTAAGGTTGTTCCCTTCCCCCTCTTGATTCTTGCTAAAATCAGTACAAGTTTCTATTTGAGATTAACTGGGAAAAGGTCTTCTCAATGTTCTAACTCCCGCGCTGGACCGGATGTAGACCAAACTATCTCGGGATGTTCAAAATCCAACTCACCTCTTGATTTAATGGAAGAATGATCACGTACGGTTCCCTGAGAAGGGATTGACTACCTACTGGAGCTTCGACCGACTATCATCGGTCAATTCGGCTTTGGGGCCCCGACCTTTTTCTAACCCAAGTGATGAAGAAATGATGTGATTACTACTAAAATAAAAACGATATTTATCGATCTCTCTTAATTCAACAATCCAAGATGTGTTCAAAGTGTTCCTCACAACCTATTTAGGAAAGTGTAACGGTCCAAGCCCACCAATAGCAGATATTGTCCTGCTTTCCCTTTCAAGCTTTCCCTCAAGATTTTTAATACGCGTCTGCTAGGAAGAGATTTCCACACTCTTACAAATAATGCTTTATTCTCCTCTCCAACCGATGTGACATTTGACAGAAAGACTAAACTCAGTTATTAGATGAGACGGATGATTTCATATTTGGGTTAGTAGTGACAGACCAGTCTAATAATAGATGTCTTGTGATTGATTTCCCTTCCTGGTGATAATTGACCAAAAGTTCTCCGAGTCTCCGACAGACATTACGAACCAGTAATAACTTGTATCATTTCTGGCATGAACAGGTCTACATATACTCGAGCGGAAGCAGGTTGGCACAAAGGCTTATATTCGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGTAACCGATTGCTGTTCTTTTCATGTTATAATCATACTCGATATCACGACCATGATTGTGGAACTGCTCTTGTATCTCAGGAACAAAAGAGAAATAAGGAGTTATGTTGAGATTTCAGAGTCTCTTGGAGTTGATACACCATCAGAGATCCTGTTCATCACAGATGTCTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGTAATGAGTCTCCCTACTTTTAGCTATTCTTTGCTGTTAGTTTATGGACGAAAAATTAGTAGAACCCGTTCGTGCTATATCGGAACTGCATTTGGCCGAAACAAAGTTCTTGTGATAAACTATCATATGGAGCTTCAAAAGAATTGCAGATTTACTTGTTTCATAATTATTCAAATGCAATCCACGACGTTTCAGGGCTGCAGGTGGCCATCTCAATCCGACCAGGAAATGGTCCACTGCCTGATGATCATGGGTTCCAGACTGCCACTTCCTTCTCAGACATCACAGCTTGAGAGACTGTTCCAAGTAAGTTTAGCCTATTTTCTCCCATCTCTGCTAGTGATTGCTTGAAGTTTAAACTGAAGATATATATATATATATAGCTGTTCTTAATCTTGTCCTGGTCAAGCCTATGTAATAAAACTGAGTTCCAAATGCCAGTTTTTTGCTTCTGAAGTATTGTGGTTACTCTGCCC

mRNA sequence

ATGGCGATGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCTCTAGCTTATGTGGACACCAAGGCTGCAAACCATACGAAGGCTCTGATGGCGGAGCTTTCCCGCCAATTCTACGGCCTCCGATTGGTTTCCGGCCCCGCCGGTTGCATTTCCATCAAGGTTCACGACCACTCCATTCCCAAGGGACAACACACAGTCGCTTTGTCGCATTCCGGTGTTAAGAAGGAGCGAATGGTAGCGGAGGACACCTGCATGTTATCTTATGGTGGGTCAATCTTATATTCACCACCTCCAAAGCCATTTTCTCACAAGCTCCCGTACTGTTTGGATGGTGGACCTCTTTTTATGAAGGATCCTTTTGGGAGCATCAAAGGAATGTCAAGCAAAATTTCTGTGAAGGCTGGGGCCCAAGGTTTAGATAATGAAAACAAACCATTGCCACGTTGCATAGTTCTTGATATCGAAGGGACTACTACACCAATATCTTTCGTTACTGACGTTCTCTTTCCATATGCTCGTGATAATGTTGGAAAGCATTTGATTTTAACGTATGAAACTGCAGAAACTCAAGATGACATTAAGTTATTGCGTTCACAAGTTGAAGAAGATTTGGAAAAGGGTGTTGCTGGAGCTGTGCCCATACCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAAGCAATGATAAAAGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGGTCACATATGGAGAACAGGATTTGCAGGGAATGAATTGGAGGGAATAGTTTTTGATGATGTGCCAGAAGCTCTTGAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATACTCGAGCGGAAGCAGGTTGGCACAAAGGCTTATATTCGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAGGAGTTATGTTGAGATTTCAGAGTCTCTTGGAGTTGATACACCATCAGAGATCCTGTTCATCACAGATGTCTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCCATCTCAATCCGACCAGGAAATGGTCCACTGCCTGATGATCATGGGTTCCAGACTGCCACTTCCTTCTCAGACATCACAGCTTGAGAGACTGTTCCAAGTAAGTTTAGCCTATTTTCTCCCATCTCTGCTAGTGATTGCTTGAAGTTTAAACTGAAGATATATATATATATATAGCTGTTCTTAATCTTGTCCTGGTCAAGCCTATGTAATAAAACTGAGTTCCAAATGCCAGTTTTTTGCTTCTGAAGTATTGTGGTTACTCTGCCC

Coding sequence (CDS)

ATGGCGATGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCACCTCTGGCCGTCAATGGAGTCAAGTTGGCTACGGCTTCTCTAGCTTATGTGGACACCAAGGCTGCAAACCATACGAAGGCTCTGATGGCGGAGCTTTCCCGCCAATTCTACGGCCTCCGATTGGTTTCCGGCCCCGCCGGTTGCATTTCCATCAAGGTTCACGACCACTCCATTCCCAAGGGACAACACACAGTCGCTTTGTCGCATTCCGGTGTTAAGAAGGAGCGAATGGTAGCGGAGGACACCTGCATGTTATCTTATGGTGGGTCAATCTTATATTCACCACCTCCAAAGCCATTTTCTCACAAGCTCCCGTACTGTTTGGATGGTGGACCTCTTTTTATGAAGGATCCTTTTGGGAGCATCAAAGGAATGTCAAGCAAAATTTCTGTGAAGGCTGGGGCCCAAGGTTTAGATAATGAAAACAAACCATTGCCACGTTGCATAGTTCTTGATATCGAAGGGACTACTACACCAATATCTTTCGTTACTGACGTTCTCTTTCCATATGCTCGTGATAATGTTGGAAAGCATTTGATTTTAACGTATGAAACTGCAGAAACTCAAGATGACATTAAGTTATTGCGTTCACAAGTTGAAGAAGATTTGGAAAAGGGTGTTGCTGGAGCTGTGCCCATACCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGCTAATGTTGAAGCAATGATAAAAGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGGTCACATATGGAGAACAGGATTTGCAGGGAATGAATTGGAGGGAATAGTTTTTGATGATGTGCCAGAAGCTCTTGAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATACTCGAGCGGAAGCAGGTTGGCACAAAGGCTTATATTCGGAAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAGGAGTTATGTTGAGATTTCAGAGTCTCTTGGAGTTGATACACCATCAGAGATCCTGTTCATCACAGATGTCTATCAGGAAGCTGTAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCCATCTCAATCCGACCAGGAAATGGTCCACTGCCTGATGATCATGGGTTCCAGACTGCCACTTCCTTCTCAGACATCACAGCTTGA

Protein sequence

MAMASPLAVNGVKLATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Homology
BLAST of CmoCh16G004330 vs. ExPASy Swiss-Prot
Match: E0CSI1 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 OS=Vitis vinifera OX=29760 GN=VIT_19s0014g02480 PE=3 SV=2)

HSP 1 Score: 503.1 bits (1294), Expect = 3.2e-141
Identity = 280/513 (54.58%), Postives = 322/513 (62.77%), Query Frame = 0

Query: 34  AVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQ 93
           A+NG+K+A  S AY+++   N T+ L+++L R FYGL  VSG  G I+IKVHD SIPK Q
Sbjct: 5   ALNGLKMAATSQAYLESMPVNDTRKLLSDLCRHFYGLGWVSGTGGSITIKVHDESIPKPQ 64

Query: 94  HTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMK------ 153
             + +S SGV+KERMV ED  +LS  G  L SP PKP+ +K P C D  PLFMK      
Sbjct: 65  QLIVMSPSGVQKERMVPEDMYVLSPSGCFLSSPSPKPYPNKPPKCSDCAPLFMKAYLMRN 124

Query: 154 ------------------DPF--------------------------------------- 213
                             DP                                        
Sbjct: 125 AGAVIHSHGMESCIVTMIDPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAHERELT 184

Query: 214 ------------------------------------------------------------ 273
                                                                       
Sbjct: 185 DALAEAIEAYPKTTAVLVRNHGIYIWGDSWIHAKTQAECYHYLFDAAIKLYQLGLDWSTP 244

Query: 274 --GSIK--GMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 333
             G IK  G  + +SVKAG    ++E +P  RCIVLDIEGTTTPISFVTDVLFP+AR+NV
Sbjct: 245 DHGPIKKYGGKTNMSVKAGTVNSNHETEPSRRCIVLDIEGTTTPISFVTDVLFPFARNNV 304

Query: 334 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 393
            +HL  TYET ETQDDIKLLR+QV+ DLE+G+ GAVPIPPD+AGKEEVIAALVANVEAMI
Sbjct: 305 SRHLAATYETDETQDDIKLLRTQVQSDLEQGIVGAVPIPPDDAGKEEVIAALVANVEAMI 364

Query: 394 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 420
           KADRKITALKQLQGHIWRTGF  NELEG+VF+DVPEAL++WHA GIKVYIYSSGSRLAQR
Sbjct: 365 KADRKITALKQLQGHIWRTGFQNNELEGVVFEDVPEALQKWHASGIKVYIYSSGSRLAQR 424

BLAST of CmoCh16G004330 vs. ExPASy Swiss-Prot
Match: B9N1F9 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Populus trichocarpa OX=3694 GN=POPTR_0001s40980g PE=3 SV=2)

HSP 1 Score: 491.1 bits (1263), Expect = 1.2e-137
Identity = 277/518 (53.47%), Postives = 324/518 (62.55%), Query Frame = 0

Query: 27  LATASP-LAVNGVKLA---TASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISI 86
           +A A P +AVNG  +A    AS AY+++KA   T+ L+A+L +QFY L  VSG  G I+I
Sbjct: 1   MAAAPPAVAVNGGGMAAAKVASQAYLESKAVKDTRVLIADLCKQFYTLGWVSGTGGSITI 60

Query: 87  KVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGG 146
           K HD SIPK Q  + +S SGV+KERM  ED  +L+  GSIL SP PKP+ +K P C D  
Sbjct: 61  KAHDDSIPKRQQLILMSPSGVQKERMEPEDMYVLATNGSILSSPSPKPYPYKPPKCSDCA 120

Query: 147 PLFMK------------------------DPFGS---------IKGMS------------ 206
           PLF+K                        +P            IKG+             
Sbjct: 121 PLFLKAYDMRNAGAVIHSHGMESCLVTMINPLSKEFRITHMEMIKGIQGHGYYDELVVPI 180

Query: 207 ------------------------------------------------------------ 266
                                                                       
Sbjct: 181 IENTAYENELTDSLAKAIEAYPKTTAVLVRNHGIYIWGDSWISAKTQAECYHYLFDAAIK 240

Query: 267 ----------------SKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 326
                             + VKAG    +N  +PLPRCIVLDIEGTTTPI+FV DVLFPY
Sbjct: 241 LHQIGLDWSTPNHGPIQNVKVKAGMNNSNNRIEPLPRCIVLDIEGTTTPITFVADVLFPY 300

Query: 327 ARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 386
           ARDNVG+HL  TY+TAET+DDI LLR+QVE+DL +GV GA+PIP D+AGKEEVIAALVAN
Sbjct: 301 ARDNVGRHLSATYDTAETKDDINLLRTQVEDDLAQGVDGAIPIPTDDAGKEEVIAALVAN 360

Query: 387 VEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 420
           VEAMIKADRKITALKQLQGHIWRTG+  NELEG+V+DDVPEALE+WHALGIKVYIYSSGS
Sbjct: 361 VEAMIKADRKITALKQLQGHIWRTGYENNELEGVVYDDVPEALEKWHALGIKVYIYSSGS 420

BLAST of CmoCh16G004330 vs. ExPASy Swiss-Prot
Match: B9SQI7 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Ricinus communis OX=3988 GN=RCOM_0591060 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.6e-135
Identity = 278/525 (52.95%), Postives = 318/525 (60.57%), Query Frame = 0

Query: 28  ATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDH 87
           A A  +AVNG  +  AS AY+++KA   T+ L+++L RQFY L  VSG  G I+IKVHD 
Sbjct: 4   AAAPAVAVNG-GVKVASQAYLESKAVKETRVLISDLCRQFYNLGWVSGTGGSITIKVHDD 63

Query: 88  SIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMK 147
           SIPK    + +S SGV+KERM  ED  +L+  GSIL SP PKP+ HK P C D GPLF+K
Sbjct: 64  SIPKPNQLILMSPSGVQKERMEPEDMYVLAANGSILSSPSPKPYPHKPPKCSDCGPLFLK 123

Query: 148 ------------------------DPFGS---------IKGMSS---------------- 207
                                   +P            IKG+                  
Sbjct: 124 AYEMCNAGAVIHSHGMESCLVTMINPLSKEFKITHMEMIKGIKGHGYYDELVVPIIENTA 183

Query: 208 ------------------------------------------------------------ 267
                                                                       
Sbjct: 184 YENELTDSLAKAIEEYPKTTAVLVRNHGIYIWGDSWISAKTQAECYHYLFDAAIKLHQLG 243

Query: 268 ------------------------KISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFV 327
                                     SVKAG +  ++  + LP CIVLDIEGTTTPI+FV
Sbjct: 244 LDWSTPNHGLIQNVKALIGSNRDINTSVKAGLKDSNHGMQSLPGCIVLDIEGTTTPITFV 303

Query: 328 TDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEV 387
            DVLFPYARDNVG+HL  TYETAETQDDIKLLR+QVE+DL +GV  AV IPPD+AGKEEV
Sbjct: 304 ADVLFPYARDNVGRHLYATYETAETQDDIKLLRTQVEDDLARGVNEAVSIPPDDAGKEEV 363

Query: 388 IAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKV 420
           IAALVANVEAMIKADRKITALKQLQGHIWRTGF  NELEG+V+DDVPEALE+WHALGIKV
Sbjct: 364 IAALVANVEAMIKADRKITALKQLQGHIWRTGFQNNELEGVVYDDVPEALEKWHALGIKV 423

BLAST of CmoCh16G004330 vs. ExPASy Swiss-Prot
Match: Q9FN41 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Arabidopsis thaliana OX=3702 GN=At5g53850 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 2.3e-120
Identity = 251/507 (49.51%), Postives = 304/507 (59.96%), Query Frame = 0

Query: 33  LAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSIPKG 92
           +AV    +     AY++ K    T +L+ EL R FY    VSG  G I++KVHD SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 93  QHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMK----- 152
           +  + +S SGV+KERM  ED  +LS  GSI+ +P PKP+ +K P C D  PLFMK     
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMR 120

Query: 153 ----------------------------DPFGSIKGM----------------------- 212
                                            IKG+                       
Sbjct: 121 NAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENEL 180

Query: 213 ----------------------------SSKISVKAGAQ---------------GLD--- 272
                                        S I  K  A+               GLD   
Sbjct: 181 TDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAAT 240

Query: 273 NENKPLPRC------------------IVLDIEGTTTPISFVTDVLFPYARDNVGKHLIL 332
            ++ P+ R                   IVLDIEGTTTPI+FVTDVLFPYAR+NVGKHL L
Sbjct: 241 PDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNL 300

Query: 333 TYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKI 392
           TY TAETQ+DIKLLR+QVEEDL +GV GAVPIP  + GKE+VIAA+V+NVEAMI+ADRKI
Sbjct: 301 TYHTAETQEDIKLLRAQVEEDLREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKI 360

Query: 393 TALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKT 420
           TALK+LQGHIWRTGF  +EL+ IVF+DV +ALE+WH+ GIKVYIYSSGSRLAQ+L+FG T
Sbjct: 361 TALKELQGHIWRTGFECDELKAIVFEDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNT 420

BLAST of CmoCh16G004330 vs. ExPASy Swiss-Prot
Match: E0CTF3 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 2 OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03470 PE=2 SV=2)

HSP 1 Score: 430.3 bits (1105), Expect = 2.6e-119
Identity = 248/520 (47.69%), Postives = 295/520 (56.73%), Query Frame = 0

Query: 35  VNGVKL-ATASLA-YVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSIPKG 94
           VNG ++  TAS A Y++T+     + L +EL R  Y L   SG  G I+IKVHD SIPK 
Sbjct: 12  VNGNEVGTTASFAGYMETRGVREARVLASELCRHMYTLGWFSGTGGSITIKVHDDSIPKP 71

Query: 95  QHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMK----- 154
           +  V +S SGV+KERMV ED  +LS  G IL +PP KP+ HK P C D  PLFMK     
Sbjct: 72  RQLVVISPSGVQKERMVPEDMYVLSSDGFILSTPPLKPYPHKPPKCTDCTPLFMKVYEMR 131

Query: 155 ------------------------------------------------------------ 214
                                                                       
Sbjct: 132 DAGAVIHSHGMESCIVTMILPFSKEFRITHMEMIKGIKGHGYHDELVVPIIENTAHEAEL 191

Query: 215 ------------------------------------------------------------ 274
                                                                       
Sbjct: 192 VESLTEAITAYPKTTAVLVRNHGVYVWGDSWISAKTQAECYHYLFDAAIKLHQLGLDWST 251

Query: 275 ---DPFGSIKGM-----SSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLF 334
               P  SI G+     +    +K G   LD+  +P  RCI+LDIEGTTTPISFVTDVLF
Sbjct: 252 PTHGPIRSINGIWGCNGTMSRGLKVGGLSLDDMIEPSQRCILLDIEGTTTPISFVTDVLF 311

Query: 335 PYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALV 394
           PYA  NVGKHL  T+++ ETQDDI LLRSQ++ DLE GV GAVPIPPD  GKE VIA+ V
Sbjct: 312 PYAHANVGKHLAATFDSEETQDDINLLRSQIQHDLEHGVVGAVPIPPDYVGKELVIASFV 371

Query: 395 ANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSS 420
           ANVEAMI+ADR ITALKQLQGHIW+TGF  NEL G+VFDDVPEALERWHA GIKVYIYSS
Sbjct: 372 ANVEAMIRADRNITALKQLQGHIWKTGFQSNELVGVVFDDVPEALERWHASGIKVYIYSS 431

BLAST of CmoCh16G004330 vs. ExPASy TrEMBL
Match: A0A6J1EAE7 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 9.3e-237
Identity = 421/421 (100.00%), Postives = 421/421 (100.00%), Query Frame = 0

Query: 1   MAMASPLAVNGVKLATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALM 60
           MAMASPLAVNGVKLATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALM
Sbjct: 1   MAMASPLAVNGVKLATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALM 60

Query: 61  AELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGG 120
           AELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGG
Sbjct: 61  AELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGG 120

Query: 121 SILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCI 180
           SILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCI
Sbjct: 121 SILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCI 180

Query: 181 VLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAG 240
           VLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAG
Sbjct: 181 VLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAG 240

Query: 241 AVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDV 300
           AVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDV
Sbjct: 241 AVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDV 300

Query: 301 PEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVE 360
           PEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVE
Sbjct: 301 PEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVE 360

Query: 361 ISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDIT 420
           ISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDIT
Sbjct: 361 ISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDIT 420

Query: 421 A 422
           A
Sbjct: 421 A 421

BLAST of CmoCh16G004330 vs. ExPASy TrEMBL
Match: A0A6J1E4Z2 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.2e-229
Identity = 406/408 (99.51%), Postives = 407/408 (99.75%), Query Frame = 0

Query: 14  LATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLV 73
           +A ASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLV
Sbjct: 1   MAMASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLV 60

Query: 74  SGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSH 133
           SGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSH
Sbjct: 61  SGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSH 120

Query: 134 KLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISF 193
           KLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISF
Sbjct: 121 KLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISF 180

Query: 194 VTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 253
           VTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE
Sbjct: 181 VTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 240

Query: 254 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIK 313
           VIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIK
Sbjct: 241 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIK 300

Query: 314 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEI 373
           VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEI
Sbjct: 301 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEI 360

Query: 374 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 422
           LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Sbjct: 361 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 408

BLAST of CmoCh16G004330 vs. ExPASy TrEMBL
Match: A0A6J1E8E3 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.3e-222
Identity = 393/395 (99.49%), Postives = 394/395 (99.75%), Query Frame = 0

Query: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86
           +A ASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 60

Query: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146
           HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM
Sbjct: 61  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 120

Query: 147 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 206
           KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV
Sbjct: 121 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 180

Query: 207 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 266
           GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI
Sbjct: 181 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 240

Query: 267 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 326
           KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR
Sbjct: 241 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 300

Query: 327 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 386
           LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA
Sbjct: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 360

Query: 387 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 422
           KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Sbjct: 361 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 395

BLAST of CmoCh16G004330 vs. ExPASy TrEMBL
Match: A0A6J1J246 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 OS=Cucurbita maxima OX=3661 GN=LOC111482722 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.6e-212
Identity = 374/395 (94.68%), Postives = 386/395 (97.72%), Query Frame = 0

Query: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86
           +A ASPLAVNGVKLAT SLAYVDTKAAN+TKALMAELSRQFYGLRLVS PAG I+IKVHD
Sbjct: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60

Query: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146
           HSIPKGQHT++LSHSGVKKERM+AEDTCMLSYGGSILYSPPPKPFSHKLP CLDG PLFM
Sbjct: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120

Query: 147 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 206
           KDPFGSIKG+SSKISVKAGAQGL+NENKPLPRCIVLDIEGTTTPISFVTD+LFPYARDNV
Sbjct: 121 KDPFGSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNV 180

Query: 207 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 266
           GKHLILTYETAETQDDI LLRSQVEEDL+KGVA AVP+PPDNAGKEEVIAALVANVEAMI
Sbjct: 181 GKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMI 240

Query: 267 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 326
           KADRKI ALKQLQGHIWRTGF+GNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR
Sbjct: 241 KADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 300

Query: 327 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 386
           LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA
Sbjct: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 360

Query: 387 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 422
           KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Sbjct: 361 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 395

BLAST of CmoCh16G004330 vs. ExPASy TrEMBL
Match: A0A1S3C2D2 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496078 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.5e-182
Identity = 329/393 (83.72%), Postives = 353/393 (89.82%), Query Frame = 0

Query: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86
           +A AS L ++GVKLAT S AY++TKAAN TKAL++ELSRQFY LR VSG    I+IKVHD
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146
           HSIP+ Q  +A+S SGVKK RMV EDT MLS GGSIL+SPPPKPFSHKLP C D  PLFM
Sbjct: 61  HSIPRRQQLIAMSLSGVKKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFM 120

Query: 147 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 206
           K   GSIKG+SS ISVKAGA+GLDN+N+  PRCIVLDIEGTTTPISFVTDVLF YARDNV
Sbjct: 121 KGQIGSIKGISSNISVKAGARGLDNKNQWFPRCIVLDIEGTTTPISFVTDVLFSYARDNV 180

Query: 207 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 266
            KHLILTYET ETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAA+VANVE MI
Sbjct: 181 EKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAAVVANVEGMI 240

Query: 267 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 326
           KADRKITALKQLQGHIWRTGF+GNELEG+VFDDVPEALERWHA GIK+YIYSSGSRLAQR
Sbjct: 241 KADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIKMYIYSSGSRLAQR 300

Query: 327 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 386
           LIFGKTNYGDLRKYLSGFFDTAVGNKRE  SYVEISES+GVD+PSEILFITDV QEA AA
Sbjct: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEISESVGVDSPSEILFITDVCQEAKAA 360

Query: 387 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 420
           KAAGLQVAISIRPGNGPLPD+HGFQT TSFS+I
Sbjct: 361 KAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 393

BLAST of CmoCh16G004330 vs. NCBI nr
Match: XP_022923004.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucurbita moschata] >XP_022923005.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucurbita moschata] >XP_022923006.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 828.9 bits (2140), Expect = 1.9e-236
Identity = 421/421 (100.00%), Postives = 421/421 (100.00%), Query Frame = 0

Query: 1   MAMASPLAVNGVKLATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALM 60
           MAMASPLAVNGVKLATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALM
Sbjct: 1   MAMASPLAVNGVKLATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALM 60

Query: 61  AELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGG 120
           AELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGG
Sbjct: 61  AELSRQFYGLRLVSGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGG 120

Query: 121 SILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCI 180
           SILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCI
Sbjct: 121 SILYSPPPKPFSHKLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCI 180

Query: 181 VLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAG 240
           VLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAG
Sbjct: 181 VLDIEGTTTPISFVTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAG 240

Query: 241 AVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDV 300
           AVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDV
Sbjct: 241 AVPIPPDNAGKEEVIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDV 300

Query: 301 PEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVE 360
           PEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVE
Sbjct: 301 PEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVE 360

Query: 361 ISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDIT 420
           ISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDIT
Sbjct: 361 ISESLGVDTPSEILFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDIT 420

Query: 421 A 422
           A
Sbjct: 421 A 421

BLAST of CmoCh16G004330 vs. NCBI nr
Match: XP_022923007.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 803.9 bits (2075), Expect = 6.6e-229
Identity = 406/408 (99.51%), Postives = 407/408 (99.75%), Query Frame = 0

Query: 14  LATASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLV 73
           +A ASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLV
Sbjct: 1   MAMASPLAVNGVKLATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLV 60

Query: 74  SGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSH 133
           SGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSH
Sbjct: 61  SGPAGCISIKVHDHSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSH 120

Query: 134 KLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISF 193
           KLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISF
Sbjct: 121 KLPYCLDGGPLFMKDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISF 180

Query: 194 VTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 253
           VTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE
Sbjct: 181 VTDVLFPYARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEE 240

Query: 254 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIK 313
           VIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIK
Sbjct: 241 VIAALVANVEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIK 300

Query: 314 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEI 373
           VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEI
Sbjct: 301 VYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEI 360

Query: 374 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 422
           LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Sbjct: 361 LFITDVYQEAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 408

BLAST of CmoCh16G004330 vs. NCBI nr
Match: XP_022923008.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 781.9 bits (2018), Expect = 2.7e-222
Identity = 393/395 (99.49%), Postives = 394/395 (99.75%), Query Frame = 0

Query: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86
           +A ASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 60

Query: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146
           HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM
Sbjct: 61  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 120

Query: 147 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 206
           KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV
Sbjct: 121 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 180

Query: 207 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 266
           GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI
Sbjct: 181 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 240

Query: 267 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 326
           KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR
Sbjct: 241 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 300

Query: 327 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 386
           LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA
Sbjct: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 360

Query: 387 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 422
           KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Sbjct: 361 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 395

BLAST of CmoCh16G004330 vs. NCBI nr
Match: XP_023551888.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita pepo subsp. pepo] >XP_023551890.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 751.5 bits (1939), Expect = 3.9e-213
Identity = 380/395 (96.20%), Postives = 387/395 (97.97%), Query Frame = 0

Query: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86
           +A ASPLAVNGVKLATASLAYVDTKAAN+TKALMAELSRQFYGLRLVS PAG I+IKVHD
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANNTKALMAELSRQFYGLRLVSRPAGSIAIKVHD 60

Query: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146
           HSIPKGQHTVALSHSGVKKERMVAEDT MLSYG SILYSPPPKPFSHKLP CLDGGPLFM
Sbjct: 61  HSIPKGQHTVALSHSGVKKERMVAEDTSMLSYGVSILYSPPPKPFSHKLPNCLDGGPLFM 120

Query: 147 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 206
           KDPFG IKG+SSKISVKAG QGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARD+V
Sbjct: 121 KDPFGRIKGISSKISVKAGTQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDSV 180

Query: 207 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 266
           GKHL+LTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI
Sbjct: 181 GKHLLLTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 240

Query: 267 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 326
           KADRKITALKQLQGHIWRTGF+GNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR
Sbjct: 241 KADRKITALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 300

Query: 327 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 386
           LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA
Sbjct: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 360

Query: 387 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 422
           KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Sbjct: 361 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 395

BLAST of CmoCh16G004330 vs. NCBI nr
Match: XP_022984417.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita maxima] >XP_022984418.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita maxima])

HSP 1 Score: 746.9 bits (1927), Expect = 9.6e-212
Identity = 374/395 (94.68%), Postives = 386/395 (97.72%), Query Frame = 0

Query: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86
           +A ASPLAVNGVKLAT SLAYVDTKAAN+TKALMAELSRQFYGLRLVS PAG I+IKVHD
Sbjct: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60

Query: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146
           HSIPKGQHT++LSHSGVKKERM+AEDTCMLSYGGSILYSPPPKPFSHKLP CLDG PLFM
Sbjct: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120

Query: 147 KDPFGSIKGMSSKISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPYARDNV 206
           KDPFGSIKG+SSKISVKAGAQGL+NENKPLPRCIVLDIEGTTTPISFVTD+LFPYARDNV
Sbjct: 121 KDPFGSIKGISSKISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPYARDNV 180

Query: 207 GKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMI 266
           GKHLILTYETAETQDDI LLRSQVEEDL+KGVA AVP+PPDNAGKEEVIAALVANVEAMI
Sbjct: 181 GKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVANVEAMI 240

Query: 267 KADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 326
           KADRKI ALKQLQGHIWRTGF+GNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR
Sbjct: 241 KADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQR 300

Query: 327 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 386
           LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA
Sbjct: 301 LIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQEAVAA 360

Query: 387 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 422
           KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA
Sbjct: 361 KAAGLQVAISIRPGNGPLPDDHGFQTATSFSDITA 395

BLAST of CmoCh16G004330 vs. TAIR 10
Match: AT5G53850.2 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 433.7 bits (1114), Expect = 1.7e-121
Identity = 251/507 (49.51%), Postives = 304/507 (59.96%), Query Frame = 0

Query: 33  LAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSIPKG 92
           +AV    +     AY++ K    T +L+ EL R FY    VSG  G I++KVHD SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 93  QHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMK----- 152
           +  + +S SGV+KERM  ED  +LS  GSI+ +P PKP+ +K P C D  PLFMK     
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMR 120

Query: 153 ----------------------------DPFGSIKGM----------------------- 212
                                            IKG+                       
Sbjct: 121 NAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENEL 180

Query: 213 ----------------------------SSKISVKAGAQ---------------GLD--- 272
                                        S I  K  A+               GLD   
Sbjct: 181 TDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAAT 240

Query: 273 NENKPLPRC------------------IVLDIEGTTTPISFVTDVLFPYARDNVGKHLIL 332
            ++ P+ R                   IVLDIEGTTTPI+FVTDVLFPYAR+NVGKHL L
Sbjct: 241 PDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNL 300

Query: 333 TYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVANVEAMIKADRKI 392
           TY TAETQ+DIKLLR+QVEEDL +GV GAVPIP  + GKE+VIAA+V+NVEAMI+ADRKI
Sbjct: 301 TYHTAETQEDIKLLRAQVEEDLREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKI 360

Query: 393 TALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKT 420
           TALK+LQGHIWRTGF  +EL+ IVF+DV +ALE+WH+ GIKVYIYSSGSRLAQ+L+FG T
Sbjct: 361 TALKELQGHIWRTGFECDELKAIVFEDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNT 420

BLAST of CmoCh16G004330 vs. TAIR 10
Match: AT5G53850.1 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.8e-70
Identity = 170/401 (42.39%), Postives = 209/401 (52.12%), Query Frame = 0

Query: 33  LAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSIPKG 92
           +AV    +     AY++ K    T +L+ EL R FY    VSG  G I++KVHD SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 93  QHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMK----- 152
           +  + +S SGV+KERM  ED  +LS  GSI+ +P PKP+ +K P C D  PLFMK     
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMR 120

Query: 153 ----------------------------DPFGSIKGM----------------------- 212
                                            IKG+                       
Sbjct: 121 NAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENEL 180

Query: 213 ----------------------------SSKISVKAGAQ---------------GLD--- 272
                                        S I  K  A+               GLD   
Sbjct: 181 TDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAAT 240

Query: 273 NENKPLPRC------------------IVLDIEGTTTPISFVTDVLFPYARDNVGKHLIL 314
            ++ P+ R                   IVLDIEGTTTPI+FVTDVLFPYAR+NVGKHL L
Sbjct: 241 PDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNL 300

BLAST of CmoCh16G004330 vs. TAIR 10
Match: AT5G53850.3 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 260.0 bits (663), Expect = 3.3e-69
Identity = 168/417 (40.29%), Postives = 207/417 (49.64%), Query Frame = 0

Query: 33  LAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHDHSIPKG 92
           +AV    +     AY++ K    T +L+ EL R FY    VSG  G I++KVHD SIPK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 93  QHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFMKDPFGS 152
           +  + +S SGV+KERM  ED  +LS  GSI+ +P PKP+ +K P C D  PLFMK+   S
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKNRRLS 120

Query: 153 IKGMSSKISVKA---------------------------------------GAQG----- 212
           +     K+  KA                                       G QG     
Sbjct: 121 LGKRLRKLGYKAYEMRNAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYD 180

Query: 213 ----------------LDNENKPLP----------------------------------- 272
                            D+  K +                                    
Sbjct: 181 ELVVPIIENTAYENELTDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYL 240

Query: 273 -----------------------------------------RCIVLDIEGTTTPISFVTD 314
                                                    R IVLDIEGTTTPI+FVTD
Sbjct: 241 FDAAIKLHQLGLDAATPDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTD 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E0CSI13.2e-14154.58Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
B9N1F91.2e-13753.47Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
B9SQI72.6e-13552.95Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Q9FN412.3e-12049.51Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
E0CTF32.6e-11947.69Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Match NameE-valueIdentityDescription
A0A6J1EAE79.3e-237100.00probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A6J1E4Z23.2e-22999.51probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A6J1E8E31.3e-22299.49probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A6J1J2464.6e-21294.68probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A1S3C2D26.5e-18283.72probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Match NameE-valueIdentityDescription
XP_022923004.11.9e-236100.00probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_022923007.16.6e-22999.51probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_022923008.12.7e-22299.49probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_023551888.13.9e-21396.20probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_022984417.19.6e-21294.68probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Match NameE-valueIdentityDescription
AT5G53850.21.7e-12149.51haloacid dehalogenase-like hydrolase family protein [more]
AT5G53850.11.8e-7042.39haloacid dehalogenase-like hydrolase family protein [more]
AT5G53850.33.3e-6940.29haloacid dehalogenase-like hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023943Enolase-phosphatase E1TIGRFAMTIGR01691TIGR01691coord: 178..418
e-value: 6.4E-68
score: 226.5
IPR023943Enolase-phosphatase E1SFLDSFLDF00044enolase-phosphatasecoord: 178..419
e-value: 0.0
score: 271.4
IPR023943Enolase-phosphatase E1CDDcd01629HAD_EPcoord: 179..400
e-value: 1.95981E-102
score: 300.999
IPR001303Class II aldolase/adducin N-terminalPFAMPF00596Aldolase_IIcoord: 59..129
e-value: 5.1E-5
score: 23.2
NoneNo IPR availableGENE3D1.10.720.60coord: 191..286
e-value: 7.6E-30
score: 105.7
NoneNo IPR availablePFAMPF00702Hydrolasecoord: 178..390
e-value: 2.0E-6
score: 28.2
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 178..419
e-value: 0.0
score: 271.4
NoneNo IPR availablePANTHERPTHR10640:SF8BIFUNCTIONAL METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE/ENOLASE-PHOSPHATASE E1-RELATEDcoord: 153..419
NoneNo IPR availablePANTHERPTHR10640:SF8BIFUNCTIONAL METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE/ENOLASE-PHOSPHATASE E1-RELATEDcoord: 33..147
IPR036409Class II aldolase/adducin N-terminal domain superfamilyGENE3D3.40.225.10coord: 52..138
e-value: 2.3E-9
score: 39.1
IPR036409Class II aldolase/adducin N-terminal domain superfamilySUPERFAMILY53639AraD/HMP-PK domain-likecoord: 54..130
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 287..419
e-value: 3.7E-46
score: 158.4
IPR017714Methylthioribulose-1-phosphate dehydratasePANTHERPTHR10640METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASEcoord: 153..419
IPR017714Methylthioribulose-1-phosphate dehydratasePANTHERPTHR10640METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASEcoord: 33..147
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 177..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G004330.1CmoCh16G004330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0019509 L-methionine salvage from methylthioadenosine
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016020 membrane
molecular_function GO:0043874 acireductone synthase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding