CmoCh16G003380 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G003380
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pectin lyase-like superfamily protein
Location	Cmo_Chr16: 1568035 .. 1568767 (-)
RNA-Seq Expression	CmoCh16G003380
Synteny	CmoCh16G003380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTTACCGACATTATTTTTTCAAAACTGCACTGAGCTCCGACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCATAAACGCTTGCAATGATGCCATTATCTCAGACGTTCACATCATTGCTCCCGAAAACATCCCTAACACATACGGATTCAACGTTTCTAGATCAAACAATGTTGTTATTCAAAACTCCTTAATCGAAACAGGTATCAAATTTGTATTACTTTTTAGTTTTAATTTGTCTTCAAACTGTCTTAGAATTTCATAATGGCTTTCTAATTTTATCGTAGGTGACGAATGTATTTCTCTATACAATGGATCTTTTAATATCAATATCGTCGGTGTTACTTGTGGACCTGGTTACGGTATAAGGTACGAAAACGACATTTTCCGTATTATAATTTCATTGTTTTTGTATTAATTTGTAATTATTATCTTAATTTCTATAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATTGAAAACATCAATGTAAGCAATTGCACCTTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTTCACAGGTTAATATAAGTAGTTTTTAATTTGTTCATATGCAATTTTTTTAATATATATTACCAAAAAATGACGGGTTTTCTTAACGTAACTACAGGGTGGACATGGATATGTAAGAAACGTCATATACATCTACCGTCCCGATTGTGACTTGCTTATCAAAATCTAA

mRNA sequence

ATGGTTTTACCGACATTATTTTTTCAAAACTGCACTGAGCTCCGACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCATAAACGCTTGCAATGATGCCATTATCTCAGACGTTCACATCATTGCTCCCGAAAACATCCCTAACACATACGGATTCAACGTTTCTAGATCAAACAATGTTGTTATTCAAAACTCCTTAATCGAAACAGGTGACGAATGTATTTCTCTATACAATGGATCTTTTAATATCAATATCGTCGGTGTTACTTGTGGACCTGGTTACGGTATAAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATTGAAAACATCAATGTAAGCAATTGCACCTTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTTCACAGGGTGGACATGGATATGTAAGAAACGTCATATACATCTACCGTCCCGATTGTGACTTGCTTATCAAAATCTAA

Coding sequence (CDS)

ATGGTTTTACCGACATTATTTTTTCAAAACTGCACTGAGCTCCGACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCATAAACGCTTGCAATGATGCCATTATCTCAGACGTTCACATCATTGCTCCCGAAAACATCCCTAACACATACGGATTCAACGTTTCTAGATCAAACAATGTTGTTATTCAAAACTCCTTAATCGAAACAGGTGACGAATGTATTTCTCTATACAATGGATCTTTTAATATCAATATCGTCGGTGTTACTTGTGGACCTGGTTACGGTATAAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATTGAAAACATCAATGTAAGCAATTGCACCTTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTTCACAGGGTGGACATGGATATGTAAGAAACGTCATATACATCTACCGTCCCGATTGTGACTTGCTTATCAAAATCTAA

Protein sequence

MVLPTLFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTSQGGHGYVRNVIYIYRPDCDLLIKI

Homology

[Close]

BLAST of CmoCh16G003380 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.6e-35
Identity = 73/143 (51.05%), Postives = 98/143 (68.53%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           L F++C  LRL    H++S   HI I+ CN   IS + I APE+ PNT G +V  S+NVV
Sbjct: 141 LKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVV 200

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQ+ +I TGD+CI++ +G+ NI+I G+ CGPG+GISI SLG+DG    +EN+ V NC  +
Sbjct: 201 IQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFR 260

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GT NG RIKT QGG GY R + +
Sbjct: 261 GTMNGARIKTWQGGSGYARMITF 283

[Close]

BLAST of CmoCh16G003380 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 1.9e-30
Identity = 61/141 (43.26%), Postives = 93/141 (65.96%), Query Frame = 0


Query: 8   FQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 67
           F  C  LR+   +  N+ + H++   C +    ++ + +P + PNT G +VS + N++IQ
Sbjct: 198 FVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQ 257

Query: 68  NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 127
           +S++ TGD+CIS+ +GS N+   G+TCGPG+GISI SLGED     + N+ V+  TL GT
Sbjct: 258 DSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGT 317

Query: 128 QNGLRIKTSQGGHGYVRNVIY 149
            NG+RIKT QGGHG  +N+I+
Sbjct: 318 TNGVRIKTWQGGHGMAKNIIF 338

[Close]

BLAST of CmoCh16G003380 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 3.4e-27
Identity = 60/143 (41.96%), Postives = 89/143 (62.24%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           L F N   L ++  K  N+ +  ISI  C++  +S+V + AP + PNT G +++ + N+ 
Sbjct: 195 LTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGIHITNTQNIR 254

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           +  S+I TGD+CIS+ +GS N+ I  +TCGPG+GISI SLG+D     +  + V    L 
Sbjct: 255 VSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLS 314

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GT NG+RIKT QGG G   N+I+
Sbjct: 315 GTDNGVRIKTYQGGSGTASNIIF 337

[Close]

BLAST of CmoCh16G003380 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.7e-26
Identity = 58/143 (40.56%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           L F  C  +R+     I+S + HI++ +C    IS + +IAP   PNT G ++S S  +V
Sbjct: 180 LTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIV 239

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           I N+ + TGD+CIS+   S  I+I  + CGPG+GISI SLG+   +  + +I V    + 
Sbjct: 240 IDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIIS 299

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
            T NG+RIKT QGG G V  +I+
Sbjct: 300 DTANGVRIKTWQGGSGLVSKIIF 322

[Close]

BLAST of CmoCh16G003380 vs. ExPASy Swiss-Prot
Match: P48978 (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.8e-25
Identity = 56/141 (39.72%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 8   FQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 67
           F  C  L ++     ++ + H+    C +   S + + APE+ PNT G +V+ + N+ I 
Sbjct: 225 FNRCNNLVVKNLNIQDAQQIHVIFQNCINVQASCLTVTAPEDSPNTDGIHVTNTQNITIS 284

Query: 68  NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 127
           +S+I TGD+CIS+ +GS  +    +TCGPG+GISI SLGEDG  + +  + V+   L GT
Sbjct: 285 SSVIGTGDDCISIVSGSQRVQATDITCGPGHGISIGSLGEDGSEDHVSGVFVNGAKLSGT 344

Query: 128 QNGLRIKTSQGGHGYVRNVIY 149
            NGLRIKT +GG G   N+++
Sbjct: 345 SNGLRIKTWKGGSGSATNIVF 365

[Close]

BLAST of CmoCh16G003380 vs. ExPASy TrEMBL
Match: A0A6J1E4H0 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111430533 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 4.7e-64
Identity = 127/130 (97.69%), Postives = 127/130 (97.69%), Query Frame = 0


Query: 10  NCT--ELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 69
           NCT   LRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ
Sbjct: 247 NCTPPTLRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 306

Query: 70  NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 129
           NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT
Sbjct: 307 NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 366

Query: 130 QNGLRIKTSQ 138
           QNGLRIKTSQ
Sbjct: 367 QNGLRIKTSQ 376

[Close]

BLAST of CmoCh16G003380 vs. ExPASy TrEMBL
Match: A0A6J1CIX8 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011581 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 4.1e-52
Identity = 103/143 (72.03%), Postives = 120/143 (83.92%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LF  NC  L+LRE KHINS KNHISINACND IISD+HIIAPE+ PNT G +VSRSNNV+
Sbjct: 148 LFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVL 207

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQNS + TGD+CI+L NGS NI+IVGVTCGPG+GISI SLG +  YNV+ENI+V NC LK
Sbjct: 208 IQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLK 267

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GTQNG+RIKT QGG+GY RN+ +
Sbjct: 268 GTQNGMRIKTWQGGYGYARNITF 290

[Close]

BLAST of CmoCh16G003380 vs. ExPASy TrEMBL
Match: A0A6J1CJJ6 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011582 PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 7.7e-51
Identity = 100/143 (69.93%), Postives = 120/143 (83.92%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LF  NC  L+LRE KHINS +NHISINACNDAIISD+HI+APE+ PNT G ++S SNNV+
Sbjct: 149 LFLHNCNGLQLREMKHINSGRNHISINACNDAIISDLHIMAPEDSPNTDGIDISGSNNVL 208

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQNS + TGD+CI+L NGS NINIVGVTCGPG+GISI SLGE+G YNV+E+I+V NC LK
Sbjct: 209 IQNSFMGTGDDCIALNNGSTNINIVGVTCGPGHGISIGSLGENGQYNVVEDIHVKNCLLK 268

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GTQNG+RIKT Q G+GY R + +
Sbjct: 269 GTQNGVRIKTWQNGYGYARGITF 291

[Close]

BLAST of CmoCh16G003380 vs. ExPASy TrEMBL
Match: A0A6J1DN64 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111021549 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 5.7e-46
Identity = 94/143 (65.73%), Postives = 117/143 (81.82%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LFFQNC  L+LRE KH+NS KNHISIN+CN+AIISD+HIIAP+N PNT G +++ SNNV+
Sbjct: 52  LFFQNCNGLQLREMKHVNSAKNHISINSCNNAIISDLHIIAPKNSPNTDGIDITGSNNVL 111

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQNS IETG +CI+L NGS NI+IV VTCG G+GISI SL E+G +N+IENI+V NC  K
Sbjct: 112 IQNSFIETGYDCIALNNGSTNIDIVDVTCGLGHGISIGSLRENGEFNLIENIHVRNCLFK 171

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
            T  G+ IKT QGG+GYVR++ +
Sbjct: 172 DTLYGMTIKTWQGGYGYVRDITF 194

[Close]

BLAST of CmoCh16G003380 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.3e-45
Identity = 86/143 (60.14%), Postives = 114/143 (79.72%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LFF NC  L+++  KHINS KNHISIN C+D I S +HI APE+ PNT G ++S S N+ 
Sbjct: 146 LFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFSHLHISAPEDSPNTDGIDISESTNIF 205

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           I++S + TGD+CI++ NGS NINI G+TCGPG+GISI SLG+DG YNV+EN++VSNC L+
Sbjct: 206 IEDSFMATGDDCIAINNGSSNININGITCGPGHGISIGSLGKDGEYNVVENVHVSNCLLR 265

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
            TQNG+RIKT +GG+GY +N+ +
Sbjct: 266 STQNGIRIKTWEGGYGYAKNITF 288

[Close]

BLAST of CmoCh16G003380 vs. NCBI nr
Match: KAG7014943.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 279.3 bits (713), Expect = 2.1e-71
Identity = 133/143 (93.01%), Postives = 140/143 (97.90%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LFFQNCT LRLRETKHINSVKNHISINACND +IS+VHIIAPENIPNTYGFN+SRSNNV+
Sbjct: 210 LFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRSNNVL 269

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQNSLIETGDECISLYNGS NINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK
Sbjct: 270 IQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 329

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GTQNGLRIKT++GGHGYVRNV+Y
Sbjct: 330 GTQNGLRIKTTKGGHGYVRNVVY 352

[Close]

BLAST of CmoCh16G003380 vs. NCBI nr
Match: XP_023552846.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 277.3 bits (708), Expect = 8.2e-71
Identity = 132/143 (92.31%), Postives = 140/143 (97.90%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LFFQNCT L+LRETKHINSVKNHIS+NACND +IS+VHIIAPENIPNTYGFN+SRSNNV+
Sbjct: 204 LFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISRSNNVL 263

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQNSLIETGDECISLYNGS NINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK
Sbjct: 264 IQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 323

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GTQNGLRIKT++GGHGYVRNVIY
Sbjct: 324 GTQNGLRIKTTKGGHGYVRNVIY 346

[Close]

BLAST of CmoCh16G003380 vs. NCBI nr
Match: KAG6576917.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276.9 bits (707), Expect = 1.1e-70
Identity = 132/143 (92.31%), Postives = 139/143 (97.20%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LFFQNCT LRLRETKHINSVKNHISINACND +IS+VHIIAPENIPNTYGFN+SRSNNV+
Sbjct: 54  LFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRSNNVL 113

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQNSLIETGDECISLYNGS NINIVGVTCGP YGISIRSLGEDGGYNVIENINVSNCTLK
Sbjct: 114 IQNSLIETGDECISLYNGSSNINIVGVTCGPSYGISIRSLGEDGGYNVIENINVSNCTLK 173

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GTQNGLRIKT++GGHGYVRNV+Y
Sbjct: 174 GTQNGLRIKTTKGGHGYVRNVVY 196

[Close]

BLAST of CmoCh16G003380 vs. NCBI nr
Match: XP_022922566.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 253.8 bits (647), Expect = 9.7e-64
Identity = 127/130 (97.69%), Postives = 127/130 (97.69%), Query Frame = 0


Query: 10  NCT--ELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 69
           NCT   LRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ
Sbjct: 247 NCTPPTLRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 306

Query: 70  NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 129
           NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT
Sbjct: 307 NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 366

Query: 130 QNGLRIKTSQ 138
           QNGLRIKTSQ
Sbjct: 367 QNGLRIKTSQ 376

[Close]

BLAST of CmoCh16G003380 vs. NCBI nr
Match: XP_022141107.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 8.5e-52
Identity = 103/143 (72.03%), Postives = 120/143 (83.92%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           LF  NC  L+LRE KHINS KNHISINACND IISD+HIIAPE+ PNT G +VSRSNNV+
Sbjct: 148 LFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVL 207

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQNS + TGD+CI+L NGS NI+IVGVTCGPG+GISI SLG +  YNV+ENI+V NC LK
Sbjct: 208 IQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLK 267

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GTQNG+RIKT QGG+GY RN+ +
Sbjct: 268 GTQNGMRIKTWQGGYGYARNITF 290

[Close]

BLAST of CmoCh16G003380 vs. TAIR 10
Match: AT4G32380.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.2e-37
Identity = 70/141 (49.65%), Postives = 99/141 (70.21%), Query Frame = 0


Query: 8   FQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 67
           F  C  +       INS +NHI+I   N+A +S++H+IAP + PNT G ++S S N+ I 
Sbjct: 52  FFGCQNILYNGLTQINSPRNHITILDSNNATLSNLHLIAPASSPNTDGIDISHSQNINIM 111

Query: 68  NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 127
           +S I+TGD+C+++   S+NIN+  VTCGPG+GISI SLGE G   V++N+NV +CT  GT
Sbjct: 112 SSTIKTGDDCVAIKRNSYNINVTYVTCGPGHGISIGSLGEGGASEVVQNVNVRHCTFTGT 171

Query: 128 QNGLRIKTSQGGHGYVRNVIY 149
           QNG RIKT  GG G+V+N++Y
Sbjct: 172 QNGARIKTWPGGQGFVKNILY 192

[Close]

BLAST of CmoCh16G003380 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 149.8 bits (377), Expect = 1.8e-36
Identity = 73/143 (51.05%), Postives = 98/143 (68.53%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           L F++C  LRL    H++S   HI I+ CN   IS + I APE+ PNT G +V  S+NVV
Sbjct: 141 LKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVV 200

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           IQ+ +I TGD+CI++ +G+ NI+I G+ CGPG+GISI SLG+DG    +EN+ V NC  +
Sbjct: 201 IQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFR 260

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
           GT NG RIKT QGG GY R + +
Sbjct: 261 GTMNGARIKTWQGGSGYARMITF 283

[Close]

BLAST of CmoCh16G003380 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 2.4e-36
Identity = 72/141 (51.06%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 8   FQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVVIQ 67
           F++C  LRL    H++S   HI I+ CN   IS + I APE+ PNT G +V  S+NVVIQ
Sbjct: 139 FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQ 198

Query: 68  NSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 127
           + +I TGD+CI++ +G+ NI+I G+ CGPG+GISI SLG+DG    +EN+ V NC  +GT
Sbjct: 199 DCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGT 258

Query: 128 QNGLRIKTSQGGHGYVRNVIY 149
            NG RIKT QGG GY R + +
Sbjct: 259 MNGARIKTWQGGSGYARMITF 279

[Close]

BLAST of CmoCh16G003380 vs. TAIR 10
Match: AT5G44830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 5.3e-36
Identity = 66/143 (46.15%), Postives = 99/143 (69.23%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           L FQ C +L++      NS +NHISI+ C    ++ + ++APE+ PNT G N+S S++V 
Sbjct: 88  LHFQRCNDLKIIGITSFNSPRNHISISECKRVQLTKIKLVAPEDSPNTDGINISGSSDVD 147

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           + ++ I TGD+C+++ NGS NINI  + CGPG+GIS+ SLG DG  +++EN+ V+NCT  
Sbjct: 148 VYDTFIGTGDDCVAINNGSVNINITRMNCGPGHGISVGSLGRDGEESIVENVQVTNCTFF 207

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
            T NG+RIKT   G GY RN+++
Sbjct: 208 RTDNGVRIKTWPNGKGYARNILF 230

[Close]

BLAST of CmoCh16G003380 vs. TAIR 10
Match: AT5G44840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 5.9e-35
Identity = 65/143 (45.45%), Postives = 97/143 (67.83%), Query Frame = 0


Query: 6   LFFQNCTELRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYGFNVSRSNNVV 65
           L FQ C +L +      NS KNHISI+ C    I+ + ++AP + PNT G N+S S++V 
Sbjct: 137 LHFQRCNDLMITGITSFNSPKNHISISECKRVKITKIKLVAPHDSPNTDGINISESSDVD 196

Query: 66  IQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 125
           I +++I TGD+C+++ +GS NINI  + CGPG+GIS+ S+G DG  +++EN+ V+NCT  
Sbjct: 197 IYDTVIGTGDDCVAINSGSMNINIARMNCGPGHGISVGSVGRDGEESIVENVQVTNCTFI 256

Query: 126 GTQNGLRIKTSQGGHGYVRNVIY 149
            T NG RIKT   G GY +N+++
Sbjct: 257 RTDNGARIKTWPNGKGYAKNILF 279

The following BLAST results are available for this feature:

BLAST of CmoCh16G003380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LW07	2.6e-35	51.05	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q9SFB7	1.9e-30	43.26	Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2	[more]
Q8RY29	3.4e-27	41.96	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
Q94AJ5	1.7e-26	40.56	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P48978	1.8e-25	39.72	Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1	[more]

BLAST of CmoCh16G003380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E4H0	4.7e-64	97.69	probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114305...	[more]
A0A6J1CIX8	4.1e-52	72.03	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1CJJ6	7.7e-51	69.93	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1DN64	5.7e-46	65.73	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A0A0KZ95	1.3e-45	60.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1	[more]

BLAST of CmoCh16G003380 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014943.1	2.1e-71	93.01	putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023552846.1	8.2e-71	92.31	probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo]	[more]
KAG6576917.1	1.1e-70	92.31	putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922566.1	9.7e-64	97.69	probable polygalacturonase At3g15720 [Cucurbita moschata]	[more]
XP_022141107.1	8.5e-52	72.03	probable polygalacturonase At3g15720 [Momordica charantia]	[more]

BLAST of CmoCh16G003380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32380.1	2.2e-37	49.65	Pectin lyase-like superfamily protein	[more]
AT3G15720.1	1.8e-36	51.05	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	2.4e-36	51.06	Pectin lyase-like superfamily protein	[more]
AT5G44830.1	5.3e-36	46.15	Pectin lyase-like superfamily protein	[more]
AT5G44840.1	5.9e-35	45.45	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 84..104 e-value: 6900.0 score: 1.2 coord: 34..60 e-value: 1100.0 score: 7.0 coord: 114..135 e-value: 39.0 score: 13.1 coord: 61..82 e-value: 250.0 score: 10.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 2..149 e-value: 3.1E-46 score: 159.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 6..148 e-value: 6.9E-40 score: 137.1
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 5..148
None	No IPR available	PANTHER	PTHR31375:SF232	SUBFAMILY NOT NAMED	coord: 5..148
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 6..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh16G003380.1	CmoCh16G003380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity