CmoCh16G003030 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G003030
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionZinc transport ZntB
LocationCmo_Chr16: 1395224 .. 1399046 (+)
RNA-Seq ExpressionCmoCh16G003030
SyntenyCmoCh16G003030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACATTACAAAAACATTTTCTCATGTTGAGAAAGCGTAGAGGTATTCATCGGGGGACGTAAACTTGAACTCGAGCACATGTCTTAACCCAAGGTTAAACTCGTCCAATTTTATACTGCTTATTTTATAAACTTGACCAAAAATTGTAAGATGCGTAATTCCAATATCTACGTCAAACCCTCTCAATTCTGGACTTTCGTCATGCTCCTTGTTCTCATTCTGCACCTTCGATCGGAATTTAGTCAACTCAGTTGAGATTTGTGAAATTTAAGGTAACCAATCTCGAATAATTTTAATAATTTTAATTTTTTTTTTAATGAAAGCATTTTCTCCTAATATGAATTGTATTTACATATGTTGTTAGTTGGAAACGTTGGTGGGAAAGATGAACAGCGTGCTAAATTCGCGTTGAATTAACTAAAAACCAACGAACTTTTTTAAGAACTCCCAAATTTAAGAAATGGAAATTTTATGGAATTTACCCAATTACTGGCGCCCGAGGTCATCAATGGCAAGTCCCATCCCAACAAACAAAAGACTTCTTAGTCGACATGATTCAAACGCCATATATTTAGATTATAACGTCTCATCAACAAATTCATAAACAATTGTTCGTGATTGGAAGTGATCCATTATCATACAGCGCTGCAACTCGTTTTCATTTCAACTTCCTCGAAAAACGACGGCCCGACGAACAGTTTTTGTTTAATCCTCGGCATTTCGCGGTACGGAAATTTCATCGTCTGCGAATTCATGTTTAGTAACCTACATGGTTGTTGAATGGGCTTTGACAAGGCACTGAAGGTTGCTTTTCAAATTTTGGCGATTGCGGGATTTCTTGTCGATGTTTTGCAGCTAGGGTTTTCGAGTATTTTGAGGAGGTAATTCTTTTTGATTTCCGATTCCATTACATGTTTTGTTTCTTTTGTTCTTCTGGATTGCTCTACTGATTTCAATCTGCGATTCTTCATAACTCATTATTCCATCGATTTCTCTCCGTTACTTCTAGAAATGTGTTCTTATAGAGCTACGGAATCTTAGAACAAATTGATGGGGAAATTAGAACAGATTTTGGTTTAATCTGTTAGTGATGTGGTGTTTGAAATTCCAGAACAAATCCATGGAGCTTGCTAATGGGGAGCCTGCTGCTAAGGGAGAATTGAATGAAATGGAGAGGGAGCCTTCAGGACGGCTTCTTTTGCGGGAAGGTGGTCTGTCTCGTACGCATTTTCCCGGCATGGTGAGGAAGAGAGCTTACATATTTGATGGAAACGACAACTATTATAACAAGGAATGGGATCTTACTGAGGGTAGAGGAAAGGAGTTTTGTTGGTATCATGTAGAACTTCCAAAAGGGAATCAAAAACTATCCCAATCTGCTCAATATCTCATCGATGTTCTTTGCCCGCCTTTGAATCTTCAAGATATTCTCTCCCTTGTAAGCAATGGACCCTTTTGTGGGCATGTTAATGGGGCGCTTGTGTTTAGAGTCAATTCACCTGGCCCTGCATCTAGTGATTTTACATTCAGAATTGCTGCTAGAATTACTAAGAATTCTGTCATCACCGTGTCTTTGGGCCGTGTTCCGAGATTAGGATTCTCACCGGTCGGCCAGTCTCTTCTCTCGGAAGTTCCTAGTGTGGAGAACCCCTCTCACTTGAGAGGTGAGCAAAGAGAAACTGGAGGCACTGTGATTAGGGAGCATGTTCTTGAATTCTTGTTGACAATGAATCACTCGGAGGAGGCTGATAATCCTGTGCCAGCATCCCTCTCAAATCTGGTTGTTCATATTATTGATACTCATGTGGATCATCTTCAAGATGTTGTCACTAAGCTTGAGATTGAGTTGGATTCTGTGGAGCTTGAAATGGATAAAGGTATGCTAGTTCTTTCTCTTTCTCCTTTCCCCCAACTTAGATTGAGATATAAAATCATTGGCTAGATGAAGTACTTTTGTATAGAAGTTAATGCGTAGTTGCTTTCATGTTGCATTCTCTTAGAATTCAATACCATTTGTCTACAAAAACACTATAACTCTAGATGATTTGGAACTTCCTGGATTGTTTTTCTTGTTTACAGTCCATTCGTGAGCTATGTTGAACTTTCCAGATATATTTGAAAGATGAAATGGGAAGAATCAAAGATTACAGTATATTACCTATTATCACGAGCTTCTATTCTTGTGCTGTTTTAGGTGGATTCACTTCAAAAAAACAAATGCTCGATGACCGAAGATTCCCCAAAATGAACCTTAATCTGCAGCGGCTCCTACAGGTAGTTTCTCTGTTAATTTTATTTTTACTCTCTCTCCTGTTTTAGACTAGGGTCGGTTGGAGAGAAGAACAAAGCATTCCTTGTAAGGGTGTGGAAACCTCTCCCTAGTAGAGCGTTTTAAAACCGTGAGGTTGATGACGATACGTAACGGGTCAAAGCAAATAATATCTGTTAGTAGTGGGGCTTGAGCTGTTATAAATGGTTATCAGAGCGAAACATCGGGGGGTGGATTGTGAGATCCACATTGATTGGAGAGGAAGGAAGCATTCCTTATAAGGGGGTGGAAACCTCTCCCTAACGGACGCGTTTTAAATCTTGAGGGTAAACTCGGAAGGAAAAGCCGAAGTGGACAATATCTGCTAGCGGCGAGTTTGGGCTGTTACATACGACAACCCCAGACTTTGCATACTCATCATCTGTATTTTTCATCGAGCGCAGGTGATTGCACATGGGGAACAAGTCTTTCCACGAGTGAAAGAAAAGTGTTCTTCTAAACAATGGTTTGCCAGTAAAGATATCAGCTCGCTTGAAGAACTAATCGGACGGTTAAGACGGTTGAAAGAGAACGTGGGATTTCTAGCAAACCGTGTCATTGCTATCCAGACCGGTCTCGATAGCTGGCAAGCCGAGCAAATTAACAGGAAACTCTATTATCTTTCATTTCTTTCAATCATATTCTTACCATTATCAGTCATTACTGGAGGTAAACAATCTCTTTCGATAAAAAAAAACAGTTTCTTGCCATGATCCATTTGAAGAAACTCATCCAATTGTTTCTGAACTATGTCCTGGCAGTATTCGGCATGAATGTTGGTGGAGTACCATGGACCGAGCAGAGAAATCCCGAGCTGAAAGAGGGATTTCGCAACGTCATGCTTCTCTGCGCCGTAATGCTTATATTGGTGCTTCTCTGCTTCAGTTTCCCAGCTATTTATACCTACTTAGTTATGTGGAATCGGCGGAGGAAGATGAGAAAGAGCTGGTCTCTCAATAGAAAGTCTTTTCTTAAGAGAACTGTGAAAAATGGTGCTGGCGACAGAGGTGGAGGAGGAGGCTATCTTCGGATTTGATAATATGATATCCTTTCCATGTGCTTGGTTTGTCCTACTCGTGCAAACCGAGCACTACGGTCTCGATCTCGATCTCGAATGATTTGTAAGTTAAACCCTCTAACTACCGATGTCTGTCGGATTCCGATTATCGTCGATAACTGCTAATGAAATATCATACATTTCATACTGAATCATCCTCCCTTTCCATGGAAGACTTGAAGGAACTGGGTTGCAGTTTGTTTCAGGTTCGTGAGGCTAACCAGTTTTATATATCCCCTAACAGTTATTTTTCACCATTTGTAAGCTATTTACATGTACAGTTTCTTTCATTCTTTTCTTCTGTTTTTCCTCACTTTCTCTGTATGGGAACATTGTTGTGCATTTGCTGGTTTTTGGCAGCATGAATGCTTTGATCAGTTTCTCATCACCTGGAACTACTGCTGTACTTATTTACGTTCATCAATGATACTTCCCAACTGTAATTATTCTAACA

mRNA sequence

ATGGATACATTACAAAAACATTTTCTCATGTTGAGAAAGCGTAGAGGCACTGAAGGTTGCTTTTCAAATTTTGGCGATTGCGGGATTTCTTGTCGATGTTTTGCAGCTAGGGTTTTCGAGTATTTTGAGGAGAACAAATCCATGGAGCTTGCTAATGGGGAGCCTGCTGCTAAGGGAGAATTGAATGAAATGGAGAGGGAGCCTTCAGGACGGCTTCTTTTGCGGGAAGGTGGTCTGTCTCGTACGCATTTTCCCGGCATGGTGAGGAAGAGAGCTTACATATTTGATGGAAACGACAACTATTATAACAAGGAATGGGATCTTACTGAGGGTAGAGGAAAGGAGTTTTGTTGGTATCATGTAGAACTTCCAAAAGGGAATCAAAAACTATCCCAATCTGCTCAATATCTCATCGATGTTCTTTGCCCGCCTTTGAATCTTCAAGATATTCTCTCCCTTGTAAGCAATGGACCCTTTTGTGGGCATGTTAATGGGGCGCTTGTGTTTAGAGTCAATTCACCTGGCCCTGCATCTAGTGATTTTACATTCAGAATTGCTGCTAGAATTACTAAGAATTCTGTCATCACCGTGTCTTTGGGCCGTGTTCCGAGATTAGGATTCTCACCGGTCGGCCAGTCTCTTCTCTCGGAAGTTCCTAGTGTGGAGAACCCCTCTCACTTGAGAGGTGAGCAAAGAGAAACTGGAGGCACTGTGATTAGGGAGCATGTTCTTGAATTCTTGTTGACAATGAATCACTCGGAGGAGGCTGATAATCCTGTGCCAGCATCCCTCTCAAATCTGGTTGTTCATATTATTGATACTCATGTGGATCATCTTCAAGATGTTGTCACTAAGCTTGAGATTGAGTTGGATTCTGTGGAGCTTGAAATGGATAAAGGTGGATTCACTTCAAAAAAACAAATGCTCGATGACCGAAGATTCCCCAAAATGAACCTTAATCTGCAGCGGCTCCTACAGGTGATTGCACATGGGGAACAAGTCTTTCCACGAGTGAAAGAAAAGTGTTCTTCTAAACAATGGTTTGCCAGTAAAGATATCAGCTCGCTTGAAGAACTAATCGGACGGTTAAGACGGTTGAAAGAGAACGTGGGATTTCTAGCAAACCGTGTCATTGCTATCCAGACCGGTCTCGATAGCTGGCAAGCCGAGCAAATTAACAGGAAACTCTATTATCTTTCATTTCTTTCAATCATATTCTTACCATTATCAGTCATTACTGGAGTATTCGGCATGAATGTTGGTGGAGTACCATGGACCGAGCAGAGAAATCCCGAGCTGAAAGAGGGATTTCGCAACGTCATGCTTCTCTGCGCCGTAATGCTTATATTGGTGCTTCTCTGCTTCAGTTTCCCAGCTATTTATACCTACTTAGTTATGTGGAATCGGCGGAGGAAGATGAGAAAGAGCTGGTCTCTCAATAGAAAGTCTTTTCTTAAGAGAACTGTGAAAAATGGTGCTGGCGACAGAGGTGGAGGAGGAGGCTATCTTCGGATTTGATAATATGATATCCTTTCCATGTGCTTGGTTTGTCCTACTCGTGCAAACCGAGCACTACGGTCTCGATCTCGATCTCGAATGATTTGTAAGTTAAACCCTCTAACTACCGATGTCTGTCGGATTCCGATTATCGTCGATAACTGCTAATGAAATATCATACATTTCATACTGAATCATCCTCCCTTTCCATGGAAGACTTGAAGGAACTGGGTTGCAGTTTGTTTCAGGTTCGTGAGGCTAACCAGTTTTATATATCCCCTAACAGTTATTTTTCACCATTTGTAAGCTATTTACATGTACAGTTTCTTTCATTCTTTTCTTCTGTTTTTCCTCACTTTCTCTGTATGGGAACATTGTTGTGCATTTGCTGGTTTTTGGCAGCATGAATGCTTTGATCAGTTTCTCATCACCTGGAACTACTGCTGTACTTATTTACGTTCATCAATGATACTTCCCAACTGTAATTATTCTAACA

Coding sequence (CDS)

ATGGATACATTACAAAAACATTTTCTCATGTTGAGAAAGCGTAGAGGCACTGAAGGTTGCTTTTCAAATTTTGGCGATTGCGGGATTTCTTGTCGATGTTTTGCAGCTAGGGTTTTCGAGTATTTTGAGGAGAACAAATCCATGGAGCTTGCTAATGGGGAGCCTGCTGCTAAGGGAGAATTGAATGAAATGGAGAGGGAGCCTTCAGGACGGCTTCTTTTGCGGGAAGGTGGTCTGTCTCGTACGCATTTTCCCGGCATGGTGAGGAAGAGAGCTTACATATTTGATGGAAACGACAACTATTATAACAAGGAATGGGATCTTACTGAGGGTAGAGGAAAGGAGTTTTGTTGGTATCATGTAGAACTTCCAAAAGGGAATCAAAAACTATCCCAATCTGCTCAATATCTCATCGATGTTCTTTGCCCGCCTTTGAATCTTCAAGATATTCTCTCCCTTGTAAGCAATGGACCCTTTTGTGGGCATGTTAATGGGGCGCTTGTGTTTAGAGTCAATTCACCTGGCCCTGCATCTAGTGATTTTACATTCAGAATTGCTGCTAGAATTACTAAGAATTCTGTCATCACCGTGTCTTTGGGCCGTGTTCCGAGATTAGGATTCTCACCGGTCGGCCAGTCTCTTCTCTCGGAAGTTCCTAGTGTGGAGAACCCCTCTCACTTGAGAGGTGAGCAAAGAGAAACTGGAGGCACTGTGATTAGGGAGCATGTTCTTGAATTCTTGTTGACAATGAATCACTCGGAGGAGGCTGATAATCCTGTGCCAGCATCCCTCTCAAATCTGGTTGTTCATATTATTGATACTCATGTGGATCATCTTCAAGATGTTGTCACTAAGCTTGAGATTGAGTTGGATTCTGTGGAGCTTGAAATGGATAAAGGTGGATTCACTTCAAAAAAACAAATGCTCGATGACCGAAGATTCCCCAAAATGAACCTTAATCTGCAGCGGCTCCTACAGGTGATTGCACATGGGGAACAAGTCTTTCCACGAGTGAAAGAAAAGTGTTCTTCTAAACAATGGTTTGCCAGTAAAGATATCAGCTCGCTTGAAGAACTAATCGGACGGTTAAGACGGTTGAAAGAGAACGTGGGATTTCTAGCAAACCGTGTCATTGCTATCCAGACCGGTCTCGATAGCTGGCAAGCCGAGCAAATTAACAGGAAACTCTATTATCTTTCATTTCTTTCAATCATATTCTTACCATTATCAGTCATTACTGGAGTATTCGGCATGAATGTTGGTGGAGTACCATGGACCGAGCAGAGAAATCCCGAGCTGAAAGAGGGATTTCGCAACGTCATGCTTCTCTGCGCCGTAATGCTTATATTGGTGCTTCTCTGCTTCAGTTTCCCAGCTATTTATACCTACTTAGTTATGTGGAATCGGCGGAGGAAGATGAGAAAGAGCTGGTCTCTCAATAGAAAGTCTTTTCTTAAGAGAACTGTGAAAAATGGTGCTGGCGACAGAGGTGGAGGAGGAGGCTATCTTCGGATTTGA

Protein sequence

MDTLQKHFLMLRKRRGTEGCFSNFGDCGISCRCFAARVFEYFEENKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI
Homology
BLAST of CmoCh16G003030 vs. ExPASy Swiss-Prot
Match: A1JNE0 (Zinc transport protein ZntB OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) OX=393305 GN=zntB PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 9.0e-10
Identity = 50/192 (26.04%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 261 PASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLN 320
           P +  N +V I+D   DH  + +  L  ++  +E ++ +    ++ QM   R+       
Sbjct: 140 PTNSGNWLVEIVDGLTDHTSEFIEDLHDKIIDLEDDLLEQKIPARGQMALLRK------Q 199

Query: 321 LQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAI 380
           L  L + +A    VF R+  +     W    D   ++E+  RL R  E++     R   +
Sbjct: 200 LIVLRRYMAPQRDVFSRLASE--RLPWMNDDDRRRMQEISERLGRGLEDLDSSIARTAVL 259

Query: 381 QTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNV 440
              + S  A+ +NR+ Y +S L+++FLP + +TG+FG+N+GG+P           GF   
Sbjct: 260 SDEISSLMADAMNRRTYTMSLLAMVFLPTTFLTGLFGVNLGGIPGNTD-----SFGFAT- 315

Query: 441 MLLCAVMLILVL 453
              C ++++LVL
Sbjct: 320 --FCMMLVVLVL 315

BLAST of CmoCh16G003030 vs. ExPASy Swiss-Prot
Match: Q7N3Y5 (Zinc transport protein ZntB OS=Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01) OX=243265 GN=zntB PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 3.4e-09
Identity = 47/167 (28.14%), Postives = 85/167 (50.90%), Query Frame = 0

Query: 268 VVHIIDTHVDHLQDVVTKLE---IELDSVELEM---DKGGFT-SKKQMLDDRRFPKMNLN 327
           +V ++D   D + + +  L    IEL+++ LE     +GG    +KQ++  RR+      
Sbjct: 147 LVEMVDAITDEVGNFIEDLHDNLIELENMILEQRIPGRGGLALLRKQLIILRRY------ 206

Query: 328 LQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAI 387
                  +A    VF R+  +     W + +D   ++E+  RL R  +++     R   I
Sbjct: 207 -------MAPQRDVFARLASE--KLLWMSDEDRYRMQEISDRLGRELDDLDGCIARTAII 266

Query: 388 QTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTE 428
              + S  A+ +NR+ Y +S L++IFLP + +TG+FG+N+GG+P  E
Sbjct: 267 SDEITSMMADAMNRRTYTMSLLAMIFLPTTFLTGLFGVNLGGIPGNE 298

BLAST of CmoCh16G003030 vs. ExPASy Swiss-Prot
Match: Q6D5P8 (Zinc transport protein ZntB OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=zntB PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 5.9e-09
Identity = 58/250 (23.20%), Postives = 109/250 (43.60%), Query Frame = 0

Query: 189 ITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHLRGEQRETGGTVI--------- 248
           +  +SV T   G   R   + +G+  L  + S+   ++ R +Q       I         
Sbjct: 61  VVPDSVRTALAGESVRPRVTRLGEGTLITLRSINLNANARPDQLVAVRVFITDKLIISTR 120

Query: 249 REHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDK 308
           R  +L     +   +E + P  +   N +V I +   DH  + +  L  ++  ++LE D 
Sbjct: 121 RRKILAIDEILTDLKEGNGPTDS--GNWLVSIAEALTDHTSEFIDDLHEKI--IDLEDD- 180

Query: 309 GGFTSKKQMLDDRRFPKMNLNLQR-----LLQVIAHGEQVFPRVKEKCSSKQWFASKDIS 368
                   +L+ +  P+  L L R     L + +     VF R+  +     W    D  
Sbjct: 181 --------LLEQKIPPRGELALIRKQLIVLRRYMTPQRDVFSRISGE--KLPWMQDDDRR 240

Query: 369 SLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITG 425
            ++E+  RL R  E++     R   +   + +   E +NR+ Y +S L+++FLP + +TG
Sbjct: 241 RMQEIADRLGRGLEDLDASVARTTVLSDEITALMTEAMNRRTYTMSLLAMVFLPTTFLTG 295

BLAST of CmoCh16G003030 vs. ExPASy Swiss-Prot
Match: A7FHP6 (Zinc transport protein ZntB OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) OX=349747 GN=zntB PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.5e-08
Identity = 49/192 (25.52%), Postives = 90/192 (46.88%), Query Frame = 0

Query: 261 PASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLN 320
           P    + +V I D   DH  + +  L  ++  +E ++ +     + QM   R+       
Sbjct: 140 PTGSGHWLVDIADGLTDHTSEFIEDLHDKIIDLEDDLMEQKVPPRGQMALLRK------Q 199

Query: 321 LQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAI 380
           L  L + +A    VF R+  +     W    D   ++E+  RL R  E++     R   +
Sbjct: 200 LIVLRRYMAPQRDVFSRLASE--RLPWMNDDDRRRMQEISERLGRGLEDLDGSIARTAVL 259

Query: 381 QTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNV 440
              + S  A+ +NR+ Y +S L+++FLP + +TG+FG+N+GG+P           GF   
Sbjct: 260 SDEISSLMADAMNRRTYTMSLLAMVFLPTTFLTGLFGVNLGGIPGNTD-----AFGF--- 315

Query: 441 MLLCAVMLILVL 453
            + C ++++LVL
Sbjct: 320 TIFCMMLVVLVL 315

BLAST of CmoCh16G003030 vs. ExPASy Swiss-Prot
Match: Q8ZE46 (Zinc transport protein ZntB OS=Yersinia pestis OX=632 GN=zntB PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.5e-08
Identity = 49/192 (25.52%), Postives = 90/192 (46.88%), Query Frame = 0

Query: 261 PASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLN 320
           P    + +V I D   DH  + +  L  ++  +E ++ +     + QM   R+       
Sbjct: 140 PTGSGHWLVDIADGLTDHTSEFIEDLHDKIIDLEDDLMEQKVPPRGQMALLRK------Q 199

Query: 321 LQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAI 380
           L  L + +A    VF R+  +     W    D   ++E+  RL R  E++     R   +
Sbjct: 200 LIVLRRYMAPQRDVFSRLASE--RLPWMNDDDRRRMQEISERLGRGLEDLDGSIARTAVL 259

Query: 381 QTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNV 440
              + S  A+ +NR+ Y +S L+++FLP + +TG+FG+N+GG+P           GF   
Sbjct: 260 SDEISSLMADAMNRRTYTMSLLAMVFLPTTFLTGLFGVNLGGIPGNTD-----AFGF--- 315

Query: 441 MLLCAVMLILVL 453
            + C ++++LVL
Sbjct: 320 TIFCMMLVVLVL 315

BLAST of CmoCh16G003030 vs. ExPASy TrEMBL
Match: A0A6J1E3X5 (uncharacterized protein LOC111430599 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430599 PE=4 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 7.4e-265
Identity = 461/462 (99.78%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 44  ENKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 103
           +NKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN
Sbjct: 7   QNKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 66

Query: 104 KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 163
           KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV
Sbjct: 67  KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 126

Query: 164 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 223
           NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN
Sbjct: 127 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 186

Query: 224 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 283
           PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV
Sbjct: 187 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 246

Query: 284 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 343
           TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS
Sbjct: 247 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 306

Query: 344 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS 403
           SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS
Sbjct: 307 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS 366

Query: 404 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY 463
           IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY
Sbjct: 367 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY 426

Query: 464 LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 506
           LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI
Sbjct: 427 LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 468

BLAST of CmoCh16G003030 vs. ExPASy TrEMBL
Match: A0A6J1E7F8 (uncharacterized protein LOC111430599 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430599 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.3e-263
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 48  MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 107
           MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD
Sbjct: 1   MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 60

Query: 108 LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 167
           LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL
Sbjct: 61  LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 120

Query: 168 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 227
           VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL
Sbjct: 121 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 180

Query: 228 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 287
           RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE
Sbjct: 181 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 240

Query: 288 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 347
           IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW
Sbjct: 241 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 300

Query: 348 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL 407
           FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL
Sbjct: 301 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL 360

Query: 408 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW 467
           PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW
Sbjct: 361 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW 420

Query: 468 NRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 506
           NRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI
Sbjct: 421 NRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 458

BLAST of CmoCh16G003030 vs. ExPASy TrEMBL
Match: A0A6J1J5S4 (uncharacterized protein LOC111482851 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482851 PE=4 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.1e-260
Identity = 457/464 (98.49%), Postives = 460/464 (99.14%), Query Frame = 0

Query: 44  ENKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 103
           +NKSMELANGEPAAKGELNE+EREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN
Sbjct: 7   QNKSMELANGEPAAKGELNEIEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 66

Query: 104 KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 163
           KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV
Sbjct: 67  KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 126

Query: 164 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 223
           NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN
Sbjct: 127 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 186

Query: 224 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 283
           PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV
Sbjct: 187 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 246

Query: 284 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 343
           TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS
Sbjct: 247 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 306

Query: 344 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS 403
           SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQT LDSWQAEQINRKLYYLSFLS
Sbjct: 307 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTSLDSWQAEQINRKLYYLSFLS 366

Query: 404 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY 463
           IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAI TY
Sbjct: 367 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAICTY 426

Query: 464 LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDR--GGGGGYLRI 506
           LVMWN+RRKMRKSWSLNRKSFLKRTVKNGAGDR  GGGGGYLRI
Sbjct: 427 LVMWNQRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGGGYLRI 470

BLAST of CmoCh16G003030 vs. ExPASy TrEMBL
Match: A0A6J1JB24 (uncharacterized protein LOC111482851 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482851 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.9e-259
Identity = 454/460 (98.70%), Postives = 456/460 (99.13%), Query Frame = 0

Query: 48  MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 107
           MELANGEPAAKGELNE+EREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD
Sbjct: 1   MELANGEPAAKGELNEIEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 60

Query: 108 LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 167
           LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL
Sbjct: 61  LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 120

Query: 168 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 227
           VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL
Sbjct: 121 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 180

Query: 228 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 287
           RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE
Sbjct: 181 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 240

Query: 288 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 347
           IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW
Sbjct: 241 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 300

Query: 348 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL 407
           FASKDISSLEELIGRLRRLKENVGFLANRVIAIQT LDSWQAEQINRKLYYLSFLSIIFL
Sbjct: 301 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTSLDSWQAEQINRKLYYLSFLSIIFL 360

Query: 408 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW 467
           PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAI TYLVMW
Sbjct: 361 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAICTYLVMW 420

Query: 468 NRRRKMRKSWSLNRKSFLKRTVKNGAGDR--GGGGGYLRI 506
           N+RRKMRKSWSLNRKSFLKRTVKNGAGDR  GGGGGYLRI
Sbjct: 421 NQRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGGGYLRI 460

BLAST of CmoCh16G003030 vs. ExPASy TrEMBL
Match: A0A1S3BYR1 (zinc transport protein ZntB isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494851 PE=4 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.0e-242
Identity = 423/467 (90.58%), Postives = 440/467 (94.22%), Query Frame = 0

Query: 39  FEYFEENKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGN 98
           F+   +NK MELA+ EP A GEL+EMEREPS RLLLRE GLSRT FPGMVRKRAYIFDGN
Sbjct: 3   FDRAVKNKYMELADAEPVANGELDEMEREPSARLLLREAGLSRTRFPGMVRKRAYIFDGN 62

Query: 99  DNYYNKEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGP 158
            +YYNKEWDLTEGRGKEFCWYHVELPKGNQKL+QSAQYLIDVLCPPL LQDILSLVSNGP
Sbjct: 63  GDYYNKEWDLTEGRGKEFCWYHVELPKGNQKLAQSAQYLIDVLCPPLKLQDILSLVSNGP 122

Query: 159 FCGHVNGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEV 218
           FCGHV+GALVFRVNSPGPASSDFTFRIAARIT+NSVITVSLGRVPRLGFSPVGQSLLSEV
Sbjct: 123 FCGHVDGALVFRVNSPGPASSDFTFRIAARITENSVITVSLGRVPRLGFSPVGQSLLSEV 182

Query: 219 PSVENPSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDH 278
           PSVE PSH RGEQRE+GG VIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTH+DH
Sbjct: 183 PSVETPSHFRGEQRESGGIVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHMDH 242

Query: 279 LQDVVTKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRV 338
           LQDVVTKLEIELDSVELEMDKGGF  KKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRV
Sbjct: 243 LQDVVTKLEIELDSVELEMDKGGFALKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRV 302

Query: 339 KEKCSSKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYY 398
           +EKCSSKQWFAS+DIS+LEELIGRLRRLKENVGF+ANRV AIQ GLDSWQAEQINRKLYY
Sbjct: 303 REKCSSKQWFASEDISALEELIGRLRRLKENVGFIANRVTAIQAGLDSWQAEQINRKLYY 362

Query: 399 LSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFP 458
           LSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVM LC VML+LVLLCFSFP
Sbjct: 363 LSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMFLCVVMLLLVLLCFSFP 422

Query: 459 AIYTYLVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 506
           A+YTYL  WNRRRKMRKSWSLNRKSFLKRTV+NG  DR  GGGYLRI
Sbjct: 423 ALYTYLATWNRRRKMRKSWSLNRKSFLKRTVRNGVEDR--GGGYLRI 467

BLAST of CmoCh16G003030 vs. NCBI nr
Match: XP_022922657.1 (uncharacterized protein LOC111430599 isoform X1 [Cucurbita moschata] >XP_023552472.1 uncharacterized protein LOC111810122 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.5e-264
Identity = 461/462 (99.78%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 44  ENKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 103
           +NKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN
Sbjct: 7   QNKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 66

Query: 104 KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 163
           KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV
Sbjct: 67  KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 126

Query: 164 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 223
           NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN
Sbjct: 127 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 186

Query: 224 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 283
           PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV
Sbjct: 187 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 246

Query: 284 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 343
           TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS
Sbjct: 247 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 306

Query: 344 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS 403
           SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS
Sbjct: 307 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS 366

Query: 404 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY 463
           IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY
Sbjct: 367 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY 426

Query: 464 LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 506
           LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI
Sbjct: 427 LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 468

BLAST of CmoCh16G003030 vs. NCBI nr
Match: XP_022922658.1 (uncharacterized protein LOC111430599 isoform X2 [Cucurbita moschata] >XP_023552473.1 uncharacterized protein LOC111810122 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.4 bits (2367), Expect = 1.1e-262
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 48  MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 107
           MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD
Sbjct: 1   MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 60

Query: 108 LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 167
           LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL
Sbjct: 61  LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 120

Query: 168 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 227
           VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL
Sbjct: 121 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 180

Query: 228 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 287
           RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE
Sbjct: 181 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 240

Query: 288 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 347
           IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW
Sbjct: 241 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 300

Query: 348 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL 407
           FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL
Sbjct: 301 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL 360

Query: 408 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW 467
           PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW
Sbjct: 361 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW 420

Query: 468 NRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 506
           NRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI
Sbjct: 421 NRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGYLRI 458

BLAST of CmoCh16G003030 vs. NCBI nr
Match: XP_022984616.1 (uncharacterized protein LOC111482851 isoform X1 [Cucurbita maxima])

HSP 1 Score: 908.7 bits (2347), Expect = 2.3e-260
Identity = 457/464 (98.49%), Postives = 460/464 (99.14%), Query Frame = 0

Query: 44  ENKSMELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 103
           +NKSMELANGEPAAKGELNE+EREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN
Sbjct: 7   QNKSMELANGEPAAKGELNEIEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYN 66

Query: 104 KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 163
           KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV
Sbjct: 67  KEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHV 126

Query: 164 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 223
           NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN
Sbjct: 127 NGALVFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVEN 186

Query: 224 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 283
           PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV
Sbjct: 187 PSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVV 246

Query: 284 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 343
           TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS
Sbjct: 247 TKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCS 306

Query: 344 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLS 403
           SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQT LDSWQAEQINRKLYYLSFLS
Sbjct: 307 SKQWFASKDISSLEELIGRLRRLKENVGFLANRVIAIQTSLDSWQAEQINRKLYYLSFLS 366

Query: 404 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTY 463
           IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAI TY
Sbjct: 367 IIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAICTY 426

Query: 464 LVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDR--GGGGGYLRI 506
           LVMWN+RRKMRKSWSLNRKSFLKRTVKNGAGDR  GGGGGYLRI
Sbjct: 427 LVMWNQRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGGGYLRI 470

BLAST of CmoCh16G003030 vs. NCBI nr
Match: KAG6576886.1 (hypothetical protein SDJN03_24460, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 907.5 bits (2344), Expect = 5.1e-260
Identity = 459/496 (92.54%), Postives = 463/496 (93.35%), Query Frame = 0

Query: 10  MLRKRRGTEGCFSNFGDCGISCRCFAARVFEYFEENKSMELANGEPAAKGELNEMEREPS 69
           MLRKRRGTEGCFSNFGDCGISCRCFAARVFEYFEENKSMELANGEPAAKGELNEMEREPS
Sbjct: 1   MLRKRRGTEGCFSNFGDCGISCRCFAARVFEYFEENKSMELANGEPAAKGELNEMEREPS 60

Query: 70  GRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWDLTEGRGKEFCWYHVELPKGNQK 129
           GRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWDLTEGRGKEFCWYHVELPKGNQK
Sbjct: 61  GRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWDLTEGRGKEFCWYHVELPKGNQK 120

Query: 130 LSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGALVFRVNSPGPASSDFTFRIAARI 189
           LSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGALVFRVNSPGPASSDFTFRIAARI
Sbjct: 121 LSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGALVFRVNSPGPASSDFTFRIAARI 180

Query: 190 TKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHLRGEQRETGGTVIREHVLEFLLT 249
           TKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHLRGEQRETGGTVIREHVLEFLLT
Sbjct: 181 TKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHLRGEQRETGGTVIREHVLEFLLT 240

Query: 250 MNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQML 309
           MNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQML
Sbjct: 241 MNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQML 300

Query: 310 DDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKEN 369
           DDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKEN
Sbjct: 301 DDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKEN 360

Query: 370 VGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTEQR 429
           VGFLANRVIAIQT       E+ +             +   +  G       GVPWTEQR
Sbjct: 361 VGFLANRVIAIQTVSCHDPFEETHP------------IVSELCPGSIWHECRGVPWTEQR 420

Query: 430 NPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMWNRRRKMRKSWSLNRKSFLKRTV 489
           NPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMWNRRRKMRKSWSLNRKSFLKRTV
Sbjct: 421 NPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMWNRRRKMRKSWSLNRKSFLKRTV 480

Query: 490 KNGAGDRGGGGGYLRI 506
           KNGAGDRGGGGGYLRI
Sbjct: 481 KNGAGDRGGGGGYLRI 484

BLAST of CmoCh16G003030 vs. NCBI nr
Match: XP_022984618.1 (uncharacterized protein LOC111482851 isoform X2 [Cucurbita maxima])

HSP 1 Score: 902.5 bits (2331), Expect = 1.6e-258
Identity = 454/460 (98.70%), Postives = 456/460 (99.13%), Query Frame = 0

Query: 48  MELANGEPAAKGELNEMEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 107
           MELANGEPAAKGELNE+EREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD
Sbjct: 1   MELANGEPAAKGELNEIEREPSGRLLLREGGLSRTHFPGMVRKRAYIFDGNDNYYNKEWD 60

Query: 108 LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 167
           LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL
Sbjct: 61  LTEGRGKEFCWYHVELPKGNQKLSQSAQYLIDVLCPPLNLQDILSLVSNGPFCGHVNGAL 120

Query: 168 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 227
           VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL
Sbjct: 121 VFRVNSPGPASSDFTFRIAARITKNSVITVSLGRVPRLGFSPVGQSLLSEVPSVENPSHL 180

Query: 228 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 287
           RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE
Sbjct: 181 RGEQRETGGTVIREHVLEFLLTMNHSEEADNPVPASLSNLVVHIIDTHVDHLQDVVTKLE 240

Query: 288 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 347
           IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW
Sbjct: 241 IELDSVELEMDKGGFTSKKQMLDDRRFPKMNLNLQRLLQVIAHGEQVFPRVKEKCSSKQW 300

Query: 348 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTGLDSWQAEQINRKLYYLSFLSIIFL 407
           FASKDISSLEELIGRLRRLKENVGFLANRVIAIQT LDSWQAEQINRKLYYLSFLSIIFL
Sbjct: 301 FASKDISSLEELIGRLRRLKENVGFLANRVIAIQTSLDSWQAEQINRKLYYLSFLSIIFL 360

Query: 408 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAIYTYLVMW 467
           PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAI TYLVMW
Sbjct: 361 PLSVITGVFGMNVGGVPWTEQRNPELKEGFRNVMLLCAVMLILVLLCFSFPAICTYLVMW 420

Query: 468 NRRRKMRKSWSLNRKSFLKRTVKNGAGDR--GGGGGYLRI 506
           N+RRKMRKSWSLNRKSFLKRTVKNGAGDR  GGGGGYLRI
Sbjct: 421 NQRRKMRKSWSLNRKSFLKRTVKNGAGDRGGGGGGGYLRI 460

BLAST of CmoCh16G003030 vs. TAIR 10
Match: AT2G04305.1 (Magnesium transporter CorA-like family protein )

HSP 1 Score: 645.2 bits (1663), Expect = 4.4e-185
Identity = 326/426 (76.53%), Postives = 359/426 (84.27%), Query Frame = 0

Query: 80  SRTHFPGMVRKRAYIFDGNDNYYNKEWDLTEGRGKEFCWYHVELPKGNQKLSQSAQYLID 139
           S   F GMVR+RAYIFDG+  YYNKEWDL EG GKEFCWYHVELPKGNQKLSQSAQ+LID
Sbjct: 31  SSHQFSGMVRQRAYIFDGDGKYYNKEWDLAEGTGKEFCWYHVELPKGNQKLSQSAQHLID 90

Query: 140 VLCPPLNLQDILSLVSNGPFCGHVNGALVFRVNSPGPASSDFTFRIAARITKNSVITVSL 199
            LCPPL LQDIL+L SNGPFCGHV+GALVFRVNSPGPASS  TF+IAARIT+NSVITVSL
Sbjct: 91  ALCPPLKLQDILALASNGPFCGHVDGALVFRVNSPGPASSSNTFKIAARITENSVITVSL 150

Query: 200 GRVPRLGFSPVGQSLLSEVPSVENPSHLRGEQRETGGTVIREHVLEFLLTMNHSEEADNP 259
           GRVPRLGFSP+GQSLLSEVPS                 VI EHVLEFLL MNHSEEADNP
Sbjct: 151 GRVPRLGFSPMGQSLLSEVPS-----------------VIEEHVLEFLLKMNHSEEADNP 210

Query: 260 VPASLSNLVVHIIDTHVDHLQDVVTKLEIELDSVELEMDKGGFTSKKQMLDDRRFPKMNL 319
           VP S+SNLVVHIIDTHVD LQDVVT+LEIELD VELEMD+GGF  KK+MLDDRRFPK++L
Sbjct: 211 VPTSVSNLVVHIIDTHVDRLQDVVTELEIELDGVELEMDRGGFAMKKKMLDDRRFPKLHL 270

Query: 320 NLQRLLQVIAHGEQVFPRVKEKCSSKQWFASKDISSLEELIGRLRRLKENVGFLANRVIA 379
           NLQRLLQVIAHGEQVFPRVKEKCS+K WF ++DI SLEELIGRLRRLKENVGF+ANRV A
Sbjct: 271 NLQRLLQVIAHGEQVFPRVKEKCSTKPWFLAEDIHSLEELIGRLRRLKENVGFIANRVTA 330

Query: 380 IQTGLDSWQAEQINRKLYYLSFLSIIFLPLSVITGVFGMNVGGVPWTEQRNPELKEGFRN 439
           IQ GLDSWQAEQINRKLYYLSFLSIIFLPLS+ITGVFGMNVGGVPWT Q  PEL++GFRN
Sbjct: 331 IQAGLDSWQAEQINRKLYYLSFLSIIFLPLSIITGVFGMNVGGVPWTGQDKPELRDGFRN 390

Query: 440 VMLLCAVMLILVLLCFSFPAIYTYLVMWNRRRKMRKSWSLNRKSFLKRTVKNGAGDRGGG 499
           VM +C +ML+LVL CF FPA+Y+ +  W   R M++SWSLNR+SF K  + N   +R   
Sbjct: 391 VMYICLIMLVLVLCCFGFPALYSRIASWWGTRDMKRSWSLNRRSFKK--IPNIVQER--- 434

Query: 500 GGYLRI 506
            GYLR+
Sbjct: 451 KGYLRL 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A1JNE09.0e-1026.04Zinc transport protein ZntB OS=Yersinia enterocolitica serotype O:8 / biotype 1B... [more]
Q7N3Y53.4e-0928.14Zinc transport protein ZntB OS=Photorhabdus laumondii subsp. laumondii (strain D... [more]
Q6D5P85.9e-0923.20Zinc transport protein ZntB OS=Pectobacterium atrosepticum (strain SCRI 1043 / A... [more]
A7FHP66.5e-0825.52Zinc transport protein ZntB OS=Yersinia pseudotuberculosis serotype O:1b (strain... [more]
Q8ZE466.5e-0825.52Zinc transport protein ZntB OS=Yersinia pestis OX=632 GN=zntB PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E3X57.4e-26599.78uncharacterized protein LOC111430599 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1E7F85.3e-263100.00uncharacterized protein LOC111430599 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J5S41.1e-26098.49uncharacterized protein LOC111482851 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JB247.9e-25998.70uncharacterized protein LOC111482851 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A1S3BYR13.0e-24290.58zinc transport protein ZntB isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494851 P... [more]
Match NameE-valueIdentityDescription
XP_022922657.11.5e-26499.78uncharacterized protein LOC111430599 isoform X1 [Cucurbita moschata] >XP_0235524... [more]
XP_022922658.11.1e-262100.00uncharacterized protein LOC111430599 isoform X2 [Cucurbita moschata] >XP_0235524... [more]
XP_022984616.12.3e-26098.49uncharacterized protein LOC111482851 isoform X1 [Cucurbita maxima][more]
KAG6576886.15.1e-26092.54hypothetical protein SDJN03_24460, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022984618.11.6e-25898.70uncharacterized protein LOC111482851 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G04305.14.4e-18576.53Magnesium transporter CorA-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 276..296
NoneNo IPR availableCOILSCoilCoilcoord: 356..376
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 388..468
e-value: 1.8E-12
score: 48.9
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 261..387
e-value: 1.7E-11
score: 46.0
NoneNo IPR availablePANTHERPTHR47468OS08G0130000 PROTEINcoord: 48..492
NoneNo IPR availableSUPERFAMILY144083Magnesium transport protein CorA, transmembrane regioncoord: 391..455
NoneNo IPR availableSUPERFAMILY143865CorA soluble domain-likecoord: 90..381
IPR002523Mg2+ transporter protein, CorA-like/Zinc transport protein ZntBPFAMPF01544CorAcoord: 114..454
e-value: 2.5E-27
score: 96.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G003030.1CmoCh16G003030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0015693 magnesium ion transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0046873 metal ion transmembrane transporter activity