CmoCh16G003000 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G003000
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr16: 1359704 .. 1365617 (-)
RNA-Seq ExpressionCmoCh16G003000
SyntenyCmoCh16G003000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTGGTGCTCGCGCCTGTACAACCTCAGGAAACTTCAATTTCACGACTAGACTCGAATGCTTGTAACATCGGTGTGCAAGAGATTGATAAGAGCCCATTTTCGTTCTCGAAGGGTTGATCGGAGCTTCGAAATAGCAGACTATGAGGCCTGAGATCGTTCTATTTGGGGATTCTTTGACTGCGCAATCCTTCGGCTCAGGCGGATGGGGAGCTGCGCTTGCTGACACTTACTCTCGGAAGGTGGATTCTCTTTTCTTTCATTCTTTAATTTGTGTTTTCGTTCAAGTTTTTGAAGTTTTTGTGTTCGTAAGTGGGTTCTTGTTCGACTAATTTATAGGCCGATGTCCTAGTTCGTGGCTACGGCGGATACAATACTCGATGGGCTTTGTTTTTACTTCATCACATTTTTCCATTGGTATGTGTTATAGTGCGCTTTTTGTTCCTTCTCTACTTTGATTTTGAGGACTTTGTTTTTACTTGAATTTGTTTCGTTATGTTCAGTAAGTGATCTTGGTGATTATTCGCATTTTAGTACTTTTTGGGCGTTTTTTTGTAATGCAACCTATCATTGTAGCTATGTTGTGGGAATTGTGCCCGTTTCTGATTCTTACAATTGATTTCAATTTAATTGAGGAAAAATTGGAAAGTTTTGCTCAATGGGGCTAAACTTACGGGGAGATCACCCAATGAACTCAAAGTTGTGATTGAACAACAAACCAAACACATTGTTTGAAAGATTTTGGAATATTGTTGTACGTTAATGATGTTGTACACGAATATGTTTGGGAGTCTAAATACGTACCTGGTGGGATGTAACAGCCCAAGCCTACCACTAGCAGATGCCGTCCTCTTTAAGTTTTTCCTTTCGGATTTCTCCTTAAGGTTTTTTAAAACGCATATGCTAGGGAGAGGTTTTCACACTCTTATAAAGAATACTTCGTTCTCCTCTCCAACCGATGTGTGATCTCCCCTGGGATGTTGTGTAGAATTCTTGTGGTAAAGAGCCAATTATAGTTGAATTATTTGGCCCCTAGATACGTAGGATGCCGGGTTTAGTGGCCCATAATTGACTTTCCTTCACCAAAAAGCTTAATCTCTTGATGCTCAGCTCACAAGGAGTGCCTCTCGAATTGGGGGTTTTCAACCTTGCCTCATTGCAAAGTTTGTGTTTTCATGCCCCTTTGAGACCCTAAAAGAGGAACCAGTAGATATTCTTTGTACTAGCTTACACAGTCATGGGTAGAAACATCTTTGTAGGCTTCTGAAAATGATGTTTCTTGTATATTTGCATTCCAAATAATTTGCATCACGTGTAATGCAGGATATCCCAAAGGCCCCCATTGTTGTGACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTTGGGAGAACAAGTGAGAGACAACACGTACCTCTTGACGAGTACAAGGATAATCTTAAAAAGATGGTAAATCATTTGAAGGTACTGACTTCTTTCTTCACTGCTCGAAATCTTTTCGAGCATACTGTGCTAATATTCATTTTGAAGATTTAAATGACTGTAAGACATTAGTCCATCACTTTTTTTATTTCATCTGTTTATAAGTTCCCCATTGACAAGGTAGTTGCTTCAGAAAGAGAGATGTTAAAAAATATATTTATGAATTAGTTCAAAGCAGGAGTACAACTTACAATTACTGGAAATGCTTTAATAGACTTTCATATTTTCTTTTGATTTGTTCTGTTGGCCATTAATTCGGTACATTCTGAAATTGTTGATGCCTGATGGATTGGAGAGGACAGAGCCACCCTCAACTATTTCGTGCTCACGAGATCGTTAAAACACTTCAGACCATACATACTTAGATATTTCATTTAAGGGTGTATTGAAGACTGAATTAAGTGCACGTTCGTCGAGATTAGTATGAATGGTGATACTAAGAAGAAAGTCTCCATTGTACTAAAAAAAGTTCTGGCTATTTAGCTATTTGGATATGCCCCGGGAACTAATCTTAGGAGTTTAAACATTTTGGAATGGAGTTATAGAAAGGGCGAGATCTACCCTTCTAAGTGGCAGACATGTTACTTCTAAATAGGTTAGATTAGTCTAGTCACTAGTGCATTGGGTAACCACCGTTAATTGGCCCAGTTGTCTTTGACATTTGGTAAACTCGTAATTTTTAGATAATAAGTTCAAACCATGGTGACCTCCTATCTCGATAATAAACATCCTACGAGTTTTTCAAAATCAAATATCGTAGGATGAGACAGTCCACTTAGAGAGAGATTTATTGACATTTGCTTGCTGCCCAAAAAACCTAAGACTATTAGAACGAAAAGGTCTAGTGCAAACTGCTAGCGATCTGAGAAACGTTCCGACTCACTTCCTTTCTCTATTTAAAACTTTTATAAAGGTAGGTAAACAAGTTAGATTGGAATGTTAGGAACTTCTTATGGGTTTGTAGTTTGAATGTGAGAGGGTTAATGAAAGTGGGCAACCACTTGTCTGACCTGAAAGTGGTCCTCTTCCTGGGAAGAAGGTTGAATGAAATGGGAGAGTTAAACCTTCGATCAGACTTCTAGGAAGACGTCCAGCTTGCAGATTGCTCATATTCTTTGAAAATGTGATTTTGCATTAGTCTTTTGAAATTGTTTCTTTGAGGCGTTTTGGCTTATGCTTTGTCAAATCTCGGGATTGTAGAGAGATGATCAAGGAGCTCCTTCTTCAGTCGCTCTTTCGTGAGAAATGAAGGTTCTTGTGAGCTTCGGTAGCGAAGTTATTTTGTAACTATCCTTTAGGGGTCGTTTGGTATAAGGTTTAAAGGTTTATAACGGTGTTTAATTCTATTAGAGAATGTTTTTCAGATTCTTATAGGAATAAATTATTTCAATACTTCAATGTTTCCCTTCACTTGTGGGCTTGAAAATTTGTAGAAGGCTCCAACAAGTAGAAATTAATATTAACTGGGGGAGGAAATGAAATAACAAAAGTTTGAACACACGACTTTTGTTATGATACCATGTTAAATCACCACTCAACCCTAAAGTTTAAGTGATAAATTACGATAAATTTACTTATTTCAACACTTCAACCAGGGTTATAGCTAATTATGGGTGCTGTGAAATGAAAGAAATCATGTGGCCTTTAATGAGCTCAATTCAAAGGTTTCAAAAGAGTCGAGTTTCTTTTTCGTTCCTCTGGTGCACTCTTCCCAGTTTCTTATGTAATTGTAGCACTCTTTTGATTCAACCCTGTTGGATTATTTTCTTGTATCCACTTCTCCCCCATTTACATCTCATTGCATTGTGGCATTTTGGTTGCAGGAGTACTCCCCAACCACGTTGGTGATACTTATTACTCCTCCTCCTGTCGACGAGGACGGGCGTAATGAATATGCACGGTATGACTTTTTGTTTCTAATCGCTGCTCAAACTTTTTAGCCTTTTTCTGGTGTAGTGGTACGTACAACAGTCTATATATTCCATCTTTCTACTTCTCCTTCAATTAGTTGTTAGGATCGCACAACACACACTATGAACACAAAGAACAAGAGAAGGAAAATGCAAGGAAAAATATTAGCTAGATGTTTATATTGATGACTTCAAATATGAACCGTAAGAGAGAATACAAAGTGTACAGAAAGTATGTTTTCAAAGTATTGCCCAAGGGAATTATTTATGGTTCAGTTTATTATATATAACTTTTCTCTAGGTATAGTCATCTACTATATATAAGTATCTACCCTATATAATATAGCCTCTTACTAGACATAGTTGTTTACTATATATGATCATTTACTTTACATGTTATTTATCCTATATATAACTTTACCTGTATAATAGTAGATGACTCCATAACCTAACACTAGTTTCATATTAACATATAAGTTATATATCGAGTATTACCAAAATATACTTTTTCATTCTTGAACCTAATGTGTAAATGTCTGAACCTGTTGAGTCCAAATGTACTCTATGCCCCTATTACGCCCTCGCTGCAGATCATCACCGATATCGGCATTTAAAGGTGGTCTAAGAACACAATCTTCAGACCAAGACAACTATACTTATTTTAAACTCTCTTCAGCAAGTTGTCTTATTTTAAGTTTCATCAATATGCCGAAGAGAGATTTTTATCCTACTCAGACCAATACAACTTTACTTGTTCAAAAACCAATTAACGTGAAGCAGGTCTTTTCTTGATTGTGAAAATAAACTTGTGTCAATCTATCAGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCTGAGAGATCAAATGAAGTAACTGGACTTTACGCGAAACAATGCCTTGAACTAGCTAAGGAAATGGGTCTTCTCGCCATTGATCTATGGTCCAAGTTGCAGGAAACAGAGGGCTGGCAGAAAAAATTTCTCAGGTCAGTGAATCCAACTCCTAGTTCACAAGTTTTACTCATGTTTTAAATATGGCTGCTGTTATCTTTAATGTTATATGTCCGACTACACTTAAAAAGTTATTCCAAATCAATCCTAGTCTCATGAATCAAATTTGATATCTACGAGTGAGAGGGATTGTTAATGAAGATTATAAATAATTCAATGTTCTTCAATGTGATATCCCACGTTGGTTGGGGAGGAAAACGAAACACCCTTTAAAAGCGTGTAGAAACCTATCCCTAGCAGATGCATTTTAAATCCTTGAGGGGAAGCCCAAAGAGGACAATATCTGCTAGTGATGGACTTGGGCCATTACAAATGGTAACCGAGCGAGACACCGGGCGATGTGCCAACGAGGAGGCTGTTCCCCATAGGAGGGTAGACACGAGACGGTGTGTCAGTAAGGACGCTGGTTCCCCAAAGGGGGGGGGGGTGGATTTGGTAGGGGTCCCACATCGATTTGAGAAAGGAACCAATGCCAGCGAGGACTCTAGGCCTTGAAGGGGGGTGAATTGTGATATCCCACATTGGTTGGGGAGGAGAATGAAACACCCTTTAAAAGCGCGTGGAAACCTCTCCCTAGCAAACGCGTTTTAAAGCCTTGAAAGGGTCTCACATCGATTGGAGAAAGGAACAAGTGCTAGCGAGGACGCTAGGCCCTGAAGGGGGGGTGAATTGTGATATCCCACATTGGTTGGGGAGGAGAACGAAACACCCTTTAAAAGCGTGTAGAAACCTCTCCCTAGCAGACGTGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGTCTAAAGAGGACAATATCTGCTAGCGGTGGATGGGCCGTTACATTCAACTCAACTTTCAAGTGATATTAATTGTTGTGCGTTGAAATTTGTGAGTTAATGTGAGATCCCACATTAGTTGGAGAGGAGAACGAAGCATTCTTTAGAAGGGCATGAAAACCTCTCCCTAGTAGACACGTTTTAAAACCGTGAGGCTGACGATGATACGTAACGGGGCCAAAGTAGACAATATCTACTAGCGGTGGGCTTAAGCTGTTACAGTTGAACTGTTCTTATTCTAACTCAATAGCTTAAACTCTAAATCGAACGGTCACACAAGCATAGCGAAGAAGTAGAATTCCTTGAACGAACGTCCACCCCACCCCCGTTTCGTATGAACTTAGTAGTGAGGCATTGTTTGTGTTTTACATGTAGGGATGGATTGCATTTCACGCCAGAAGGCAACGTAATTCTGCACCAAGAACTAGAGAAAGTACTGAATGAAACATCAGTTTCTGCTGCAAAATTGCCTCTTGATTTCCCACATCACTCAGAAATCGATGGAAAAAGCCCCGAAAAGGCCTTCCAATATCTTTCTTTGTGATATACTTTCATCATCATATCATATCTTGTTATGGAAGAAAGCTTTATTTGTATTTGCATTTGATTGTAAACCATAATTTTCTGTCTTCCATTTGGTATCATCTTGTTTGATGAGTATGAATATTAAATATTAGAGACCAAAATGTG

mRNA sequence

GCCTGGTGCTCGCGCCTGTACAACCTCAGGAAACTTCAATTTCACGACTAGACTCGAATGCTTGTAACATCGGTGTGCAAGAGATTGATAAGAGCCCATTTTCGTTCTCGAAGGGTTGATCGGAGCTTCGAAATAGCAGACTATGAGGCCTGAGATCGTTCTATTTGGGGATTCTTTGACTGCGCAATCCTTCGGCTCAGGCGGATGGGGAGCTGCGCTTGCTGACACTTACTCTCGGAAGGCCGATGTCCTAGTTCGTGGCTACGGCGGATACAATACTCGATGGGCTTTGTTTTTACTTCATCACATTTTTCCATTGGATATCCCAAAGGCCCCCATTGTTGTGACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTTGGGAGAACAAGTGAGAGACAACACGTACCTCTTGACGAGTACAAGGATAATCTTAAAAAGATGGTAAATCATTTGAAGGAGTACTCCCCAACCACGTTGGTGATACTTATTACTCCTCCTCCTGTCGACGAGGACGGGCGTAATGAATATGCACGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCTGAGAGATCAAATGAAGTAACTGGACTTTACGCGAAACAATGCCTTGAACTAGCTAAGGAAATGGGTCTTCTCGCCATTGATCTATGGTCCAAGTTGCAGGAAACAGAGGGCTGGCAGAAAAAATTTCTCAGGGATGGATTGCATTTCACGCCAGAAGGCAACGTAATTCTGCACCAAGAACTAGAGAAAGTACTGAATGAAACATCAGTTTCTGCTGCAAAATTGCCTCTTGATTTCCCACATCACTCAGAAATCGATGGAAAAAGCCCCGAAAAGGCCTTCCAATATCTTTCTTTGTGATATACTTTCATCATCATATCATATCTTGTTATGGAAGAAAGCTTTATTTGTATTTGCATTTGATTGTAAACCATAATTTTCTGTCTTCCATTTGGTATCATCTTGTTTGATGAGTATGAATATTAAATATTAGAGACCAAAATGTG

Coding sequence (CDS)

ATGAGGCCTGAGATCGTTCTATTTGGGGATTCTTTGACTGCGCAATCCTTCGGCTCAGGCGGATGGGGAGCTGCGCTTGCTGACACTTACTCTCGGAAGGCCGATGTCCTAGTTCGTGGCTACGGCGGATACAATACTCGATGGGCTTTGTTTTTACTTCATCACATTTTTCCATTGGATATCCCAAAGGCCCCCATTGTTGTGACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTTGGGAGAACAAGTGAGAGACAACACGTACCTCTTGACGAGTACAAGGATAATCTTAAAAAGATGGTAAATCATTTGAAGGAGTACTCCCCAACCACGTTGGTGATACTTATTACTCCTCCTCCTGTCGACGAGGACGGGCGTAATGAATATGCACGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCTGAGAGATCAAATGAAGTAACTGGACTTTACGCGAAACAATGCCTTGAACTAGCTAAGGAAATGGGTCTTCTCGCCATTGATCTATGGTCCAAGTTGCAGGAAACAGAGGGCTGGCAGAAAAAATTTCTCAGGGATGGATTGCATTTCACGCCAGAAGGCAACGTAATTCTGCACCAAGAACTAGAGAAAGTACTGAATGAAACATCAGTTTCTGCTGCAAAATTGCCTCTTGATTTCCCACATCACTCAGAAATCGATGGAAAAAGCCCCGAAAAGGCCTTCCAATATCTTTCTTTGTGA

Protein sequence

MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLDIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEGWQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYLSL
Homology
BLAST of CmoCh16G003000 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 356.7 bits (914), Expect = 2.1e-97
Identity = 159/238 (66.81%), Postives = 196/238 (82.35%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDS+TAQSF SGGWG+ALAD YSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P+  TIFFGANDAA+ GRTS+RQHVP++EY DN++K+V HLK+ SPT L++LITPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P+DE GR  YA S+YG+KA + PER+NE TG+YA+ C+ LA+E+GL  ++LWSK+QET  
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQ 239
           WQKK+L DGLH TPEGN ++  E+ +V  E  +S  ++P DFPHHS IDGK+P KAF+
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKAFE 238

BLAST of CmoCh16G003000 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-62
Identity = 119/240 (49.58%), Postives = 158/240 (65.83%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60
           MR +I LFGDS+T +SF  GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  +FP+ 
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  --DIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILI 120
             D   +P  VT+FFGANDA +  R S  QHVPL EYK NL+ +V+ LK   P T +ILI
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 121 TPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQE 180
           TPPP+DE+ R  Y    Y +    LPER+NEV GLYAK C+ +A+E  +   DLWSK+Q+
Sbjct: 122 TPPPIDEEARLRYP---YIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQ 181

Query: 181 TEGWQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF 238
              WQ + L DGLH +  GN ++ +E+ K L E  + A  L +D P   ++D K P K+F
Sbjct: 182 IPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSF 238

BLAST of CmoCh16G003000 vs. ExPASy Swiss-Prot
Match: O80443 (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.6e-44
Identity = 90/227 (39.65%), Postives = 139/227 (61.23%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           +RP+IVLFG S+   SF   GWGA LAD YSR AD+++RGY G+N+R+AL +LH +FP D
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPKD 64

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P +V ++FG ND+     +    HVPL E+ +N++K+  HL   S  T VI +TPP
Sbjct: 65  AVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIFLTPP 124

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P++E    +    ++GD    +  RSNE+   YA++ L L +E+ +  ID+W+ +Q+ + 
Sbjct: 125 PMNE----KQIEIVFGD---AIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDD 184

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLN----ETSVSAAKLPLDFP 224
           W      DG+HFT + + I+ +E+ KVL     + S+    LP++FP
Sbjct: 185 WLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFP 224

BLAST of CmoCh16G003000 vs. ExPASy Swiss-Prot
Match: Q2TAA2 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.9e-42
Identity = 101/237 (42.62%), Postives = 139/237 (58.65%), Query Frame = 0

Query: 3   PEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIF----P 62
           P ++LFGDS+T  SF  GGWGA+LAD   RK DVL RG+ GYNTRWA  +L  +      
Sbjct: 16  PRLLLFGDSITQFSFQQGGWGASLADRLVRKCDVLNRGFSGYNTRWAKIILPRLIRKGNS 75

Query: 63  LDIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYS-PTTLVILI 122
           LDI   P+ VTIFFGAND+A+    + +QH+PL+EY  NLK MV +LK    P   VILI
Sbjct: 76  LDI---PVAVTIFFGANDSALKDE-NPKQHIPLEEYAANLKSMVQYLKSVDIPENRVILI 135

Query: 123 TPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQE 182
           TP P+ E    E    + G K   L    N V G YA  CL++A++ G   +DLW+ +Q+
Sbjct: 136 TPTPLCETAWEEQC-IIQGCKLNRL----NSVVGEYANACLQVAQDCGTDVLDLWTLMQD 195

Query: 183 TEGWQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPE 235
           ++ +   +L DGLH +P+GN  L   L  ++ +     + LPL  P+  ++    PE
Sbjct: 196 SQDF-SSYLSDGLHLSPKGNEFLFSHLWPLIEK---KVSSLPLLLPYWRDVAEAKPE 239

BLAST of CmoCh16G003000 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 4.7e-41
Identity = 87/232 (37.50%), Postives = 137/232 (59.05%), Query Frame = 0

Query: 2   RPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLDI 61
           RP+IVLFG S+   SFG GGWGA L++ Y+RKAD+++RGY G+N+  AL ++  +FP D 
Sbjct: 6   RPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQVFPKDA 65

Query: 62  PKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPPP 121
              P +V ++FG ND+     +    HVPL EY DN+KK+  HL+  S  T +I ++ PP
Sbjct: 66  AVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIFLSSPP 125

Query: 122 VDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEGW 181
           VDE  +    +S Y  +      R+N++   Y+  C+EL +E+GL  +DL+S  Q+ + W
Sbjct: 126 VDE-AKVRQNQSPYLSEV----IRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 185

Query: 182 QKKFLRDGLHFTPEGNVILHQELEKVLNET----SVSAAKLPLDFPHHSEID 230
           +     DG+H + +G+ I+  E+ +V+ E     S+    +P +F   S  D
Sbjct: 186 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYD 232

BLAST of CmoCh16G003000 vs. ExPASy TrEMBL
Match: A0A6J1E910 (GDSL esterase/lipase At5g62930 OS=Cucurbita moschata OX=3662 GN=LOC111430496 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 9.7e-138
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. ExPASy TrEMBL
Match: A0A6J1J450 (GDSL esterase/lipase At5g62930 OS=Cucurbita maxima OX=3661 GN=LOC111483251 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 1.5e-135
Identity = 238/242 (98.35%), Postives = 239/242 (98.76%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGL+AIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLIAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGN ILH ELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF YL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHHELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFHYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. ExPASy TrEMBL
Match: A0A6J1D5G5 (GDSL esterase/lipase At5g62930 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017779 PE=3 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 4.1e-128
Identity = 223/242 (92.15%), Postives = 236/242 (97.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRP+IVLFGDSLTAQSFGSGGWGAALADTYSRKADV+VRGYGGYN+RWALFLLHHIFPLD
Sbjct: 1   MRPQIVLFGDSLTAQSFGSGGWGAALADTYSRKADVVVRGYGGYNSRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPK PIVVTIFFGANDAAI GRTSERQHVPL+E+K+NLKKMVNHLKE SPTTLVILITPP
Sbjct: 61  IPKPPIVVTIFFGANDAAILGRTSERQHVPLEEFKNNLKKMVNHLKECSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRNEYARSLYGD+ARELPER+NEVTGLYA QCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEEGRNEYARSLYGDEARELPERTNEVTGLYATQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLH TPEGN I+HQELEKVLNETSVSAAKLPLDFPHHS+IDGK+PEKAF+YL
Sbjct: 181 WQKKFLRDGLHLTPEGNAIVHQELEKVLNETSVSAAKLPLDFPHHSKIDGKNPEKAFEYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. ExPASy TrEMBL
Match: A0A0A0KTH4 (SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.2e-127
Identity = 220/242 (90.91%), Postives = 236/242 (97.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAA+ GRTSE+QHVPL+EYK NLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL AIDLWSK+QETEG
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGN +LHQELEKVLNETSV+AAK+PLDFPHHS+IDGK+PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. ExPASy TrEMBL
Match: A0A6J1D6S7 (GDSL esterase/lipase At5g62930 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017779 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.7e-126
Identity = 223/245 (91.02%), Postives = 236/245 (96.33%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60
           MRP+IVLFGDSLTAQSFGSGGWGAALADTYSRKADV+VRGYGGYN+RWALFLLHHIFPL 
Sbjct: 1   MRPQIVLFGDSLTAQSFGSGGWGAALADTYSRKADVVVRGYGGYNSRWALFLLHHIFPLV 60

Query: 61  --DIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILI 120
             DIPK PIVVTIFFGANDAAI GRTSERQHVPL+E+K+NLKKMVNHLKE SPTTLVILI
Sbjct: 61  CADIPKPPIVVTIFFGANDAAILGRTSERQHVPLEEFKNNLKKMVNHLKECSPTTLVILI 120

Query: 121 TPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQE 180
           TPPPVDE+GRNEYARSLYGD+ARELPER+NEVTGLYA QCLELAKEMGLLAIDLWSKLQE
Sbjct: 121 TPPPVDEEGRNEYARSLYGDEARELPERTNEVTGLYATQCLELAKEMGLLAIDLWSKLQE 180

Query: 181 TEGWQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF 240
           TEGWQKKFLRDGLH TPEGN I+HQELEKVLNETSVSAAKLPLDFPHHS+IDGK+PEKAF
Sbjct: 181 TEGWQKKFLRDGLHLTPEGNAIVHQELEKVLNETSVSAAKLPLDFPHHSKIDGKNPEKAF 240

Query: 241 QYLSL 243
           +YLSL
Sbjct: 241 EYLSL 245

BLAST of CmoCh16G003000 vs. NCBI nr
Match: XP_022922525.1 (GDSL esterase/lipase At5g62930 [Cucurbita moschata])

HSP 1 Score: 499.2 bits (1284), Expect = 2.0e-137
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. NCBI nr
Match: XP_023551925.1 (GDSL esterase/lipase At5g62930 [Cucurbita pepo subsp. pepo] >KAG6576884.1 GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496.5 bits (1277), Expect = 1.3e-136
Identity = 241/242 (99.59%), Postives = 241/242 (99.59%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGN ILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. NCBI nr
Match: KAG7014907.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495.0 bits (1273), Expect = 3.8e-136
Identity = 240/242 (99.17%), Postives = 241/242 (99.59%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGN ILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. NCBI nr
Match: XP_022985167.1 (GDSL esterase/lipase At5g62930 [Cucurbita maxima])

HSP 1 Score: 491.9 bits (1265), Expect = 3.2e-135
Identity = 238/242 (98.35%), Postives = 239/242 (98.76%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGL+AIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLIAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGN ILH ELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF YL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHHELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFHYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. NCBI nr
Match: XP_038904756.1 (GDSL esterase/lipase At5g62930 isoform X2 [Benincasa hispida])

HSP 1 Score: 474.6 bits (1220), Expect = 5.3e-130
Identity = 227/242 (93.80%), Postives = 236/242 (97.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAAI GRTSERQHVPL+EYKDNLKKMVNHLKEYSPT+LVILITPP
Sbjct: 61  TPKAPIVVTIFFGANDAAILGRTSERQHVPLEEYKDNLKKMVNHLKEYSPTSLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAKQCLELAKEMGL  IDLWSKLQETEG
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKQCLELAKEMGLHVIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGN +LHQELEKVLNETSVSA+K+PLDFPHHS+IDGK PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVSASKMPLDFPHHSKIDGKHPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of CmoCh16G003000 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 1.5e-98
Identity = 159/238 (66.81%), Postives = 196/238 (82.35%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           MRPEIVLFGDS+TAQSF SGGWG+ALAD YSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P+  TIFFGANDAA+ GRTS+RQHVP++EY DN++K+V HLK+ SPT L++LITPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P+DE GR  YA S+YG+KA + PER+NE TG+YA+ C+ LA+E+GL  ++LWSK+QET  
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQ 239
           WQKK+L DGLH TPEGN ++  E+ +V  E  +S  ++P DFPHHS IDGK+P KAF+
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKAFE 238

BLAST of CmoCh16G003000 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 9.1e-64
Identity = 119/240 (49.58%), Postives = 158/240 (65.83%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60
           MR +I LFGDS+T +SF  GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  +FP+ 
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  --DIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILI 120
             D   +P  VT+FFGANDA +  R S  QHVPL EYK NL+ +V+ LK   P T +ILI
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 121 TPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQE 180
           TPPP+DE+ R  Y    Y +    LPER+NEV GLYAK C+ +A+E  +   DLWSK+Q+
Sbjct: 122 TPPPIDEEARLRYP---YIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQ 181

Query: 181 TEGWQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF 238
              WQ + L DGLH +  GN ++ +E+ K L E  + A  L +D P   ++D K P K+F
Sbjct: 182 IPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSF 238

BLAST of CmoCh16G003000 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 3.3e-45
Identity = 90/227 (39.65%), Postives = 139/227 (61.23%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60
           +RP+IVLFG S+   SF   GWGA LAD YSR AD+++RGY G+N+R+AL +LH +FP D
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPKD 64

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P +V ++FG ND+     +    HVPL E+ +N++K+  HL   S  T VI +TPP
Sbjct: 65  AVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIFLTPP 124

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P++E    +    ++GD    +  RSNE+   YA++ L L +E+ +  ID+W+ +Q+ + 
Sbjct: 125 PMNE----KQIEIVFGD---AIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDD 184

Query: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLN----ETSVSAAKLPLDFP 224
           W      DG+HFT + + I+ +E+ KVL     + S+    LP++FP
Sbjct: 185 WLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFP 224

BLAST of CmoCh16G003000 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 3.4e-42
Identity = 87/232 (37.50%), Postives = 137/232 (59.05%), Query Frame = 0

Query: 2   RPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLDI 61
           RP+IVLFG S+   SFG GGWGA L++ Y+RKAD+++RGY G+N+  AL ++  +FP D 
Sbjct: 6   RPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQVFPKDA 65

Query: 62  PKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPPP 121
              P +V ++FG ND+     +    HVPL EY DN+KK+  HL+  S  T +I ++ PP
Sbjct: 66  AVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIFLSSPP 125

Query: 122 VDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEGW 181
           VDE  +    +S Y  +      R+N++   Y+  C+EL +E+GL  +DL+S  Q+ + W
Sbjct: 126 VDE-AKVRQNQSPYLSEV----IRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 185

Query: 182 QKKFLRDGLHFTPEGNVILHQELEKVLNET----SVSAAKLPLDFPHHSEID 230
           +     DG+H + +G+ I+  E+ +V+ E     S+    +P +F   S  D
Sbjct: 186 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYD 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FM042.1e-9766.81GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2... [more]
Q6NMR91.3e-6249.58GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2... [more]
O804434.6e-4439.65GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2... [more]
Q2TAA21.9e-4242.62Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 P... [more]
Q9SRM54.7e-4137.50GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E9109.7e-138100.00GDSL esterase/lipase At5g62930 OS=Cucurbita moschata OX=3662 GN=LOC111430496 PE=... [more]
A0A6J1J4501.5e-13598.35GDSL esterase/lipase At5g62930 OS=Cucurbita maxima OX=3661 GN=LOC111483251 PE=3 ... [more]
A0A6J1D5G54.1e-12892.15GDSL esterase/lipase At5g62930 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0KTH41.2e-12790.91SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 ... [more]
A0A6J1D6S71.7e-12691.02GDSL esterase/lipase At5g62930 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_022922525.12.0e-137100.00GDSL esterase/lipase At5g62930 [Cucurbita moschata][more]
XP_023551925.11.3e-13699.59GDSL esterase/lipase At5g62930 [Cucurbita pepo subsp. pepo] >KAG6576884.1 GDSL e... [more]
KAG7014907.13.8e-13699.17GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022985167.13.2e-13598.35GDSL esterase/lipase At5g62930 [Cucurbita maxima][more]
XP_038904756.15.3e-13093.80GDSL esterase/lipase At5g62930 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G62930.11.5e-9866.81SGNH hydrolase-type esterase superfamily protein [more]
AT5G45920.19.1e-6449.58SGNH hydrolase-type esterase superfamily protein [more]
AT2G38180.13.3e-4539.65SGNH hydrolase-type esterase superfamily protein [more]
AT3G11210.13.4e-4237.50SGNH hydrolase-type esterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 2..236
e-value: 4.9E-72
score: 244.1
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 5..203
e-value: 1.6E-27
score: 97.5
NoneNo IPR availablePANTHERPTHR14209:SF19GDSL-LIKE LIPASE/ACYLHYDROLASE FAMILY PROTEIN, EXPRESSEDcoord: 1..239
NoneNo IPR availableCDDcd01838Isoamyl_acetate_hydrolase_likecoord: 5..208
e-value: 1.62221E-96
score: 278.752
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 3..209
IPR045136Isoamyl acetate-hydrolyzing esterase Iah1-likePANTHERPTHR14209ISOAMYL ACETATE-HYDROLYZING ESTERASE 1coord: 1..239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G003000.1CmoCh16G003000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds