CmoCh16G002830 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G002830
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionERAD-associated E3 ubiquitin-protein ligase HRD1B-like
LocationCmo_Chr16: 1272373 .. 1277352 (-)
RNA-Seq ExpressionCmoCh16G002830
SyntenyCmoCh16G002830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGATTGGAAAGACCATGTCATCTGAGAATTCATCGGAAAAAGTATTGAAAGAGAAAACCTAACATCGAATTTCATATTAGTTCTTCCTCAGCTCTCCCTATTCTGCAAAATTTCTGGTTCGTCTCATCTTCATCGTCTCCGAATTGGATTAGCGTCGGGATCCAGCCGGCCATAGGTAGCCGTAGACGCCGATCAATCAGGCAAGATTTCATTTTAAAAAACAACTTCTCTGTTGATATCTTTGGATTTGCTCAGTCAATTGGTTTTGTAACATCAAGATTACGAGTTTTTGGTTTACCAAGTTTAATCTGATCATGTTGCAAAAACATTACACGGCCTGAATCAATAACGAAGAAACGTCGGGGCTAGAAAAAATTGGAGGGAAAATAAAATGCCTTGCCCGGCTGGCGCTATGGATTGATGAGATGTAATTGACGACCTGTTATCAAATCTCGGAATTAAGCATAAAATGGAAATTATTATAGCCTTTGGCATTGAAGTCCATGAGATAGAAACCAGAGAATTAACTTTCCTATATAAGATCTTCATCATTGAAAAATCCCTACTTTTCCTTCAGCCAATCCAGGAAAGCTCGGATAACTTGGAGAAGGAAAACCCTCACATTATTAAAGCTTTTTATGCATTACTTCTAAGTTTAAACGTCTTACGTACAAATAACTAATTTACTGTATTTTTAGGATGGGTACCGATGATATTTTAGCATCACATATATTGTTTCATTTGGCTGACATTTCAACTTCTCATATGGCAGTGCAGTCGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTCATTGCAACCATAGTGGTTATCTATCATGCCTTCAACAGTAGAGGCCAGTTCTACCCAGCACTGGTCTATTTATCGACTTCCAAGATAAATCTAGTGCTTCTTCTCAATATGGGTCTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAGAAAATATTTCTCGGTTCTCTTAGAGAAGCAGAGGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATAACTATTTTCCGCCAAGATTTTTCTGTTTCATTCCTTGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATAGTTTCCTTCTTAGGCTTCCTCTTTGTTCTAGATAGTCTGTTTTTTTATAATTCGATAGAATCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTAAGATTTCAAATATCCTATGATGTTTTCTTATGACCCTTAGAAGCTCTTATATCATCATAAAATAAAGTCAATGACTTGTTTTTCAGGTATATGATTCTTGCAACAACGGCTGTGTCAACGTTTGTAAAATATATTTTCTATGTCAGTGATGTACTCATGGAGGGACAATGGGAAAGGAAGCCCGTCTATACATTTTACTTGGAACTTATCCGAGACTTACTTCACTTGTCAATGTACCTGTGTTTCTTTCTTGTGATTTTCATGTAAGTACTCTAGCTACACCTGTTAAGCTATCTCATTCTTCTGAACTGGAAGTTCAGAATTAATTTCTTCTGCATATATGTGCTCATATTGCGTATGCCCAAATGTTAAAGTAGTAAAACACTGGCTCTTCTAAAGAAATACTATGCAAACCAATAAAAAACGAATGTGGTTACTGGGATTTGTGTCCTTACATGTATCAATGGCTGTCGTATTTTGCTCTCATGTTAATTGTGGGAGAAGCCTCATAAATCATAACATTAGTGTCTTATGACTGACATGGTCTTTGTTGTTTGTTTGGATATCCTTTTCATTTACTTCCAATCAGTCGTTGTTCTTAATTTCTTTCCTCTTTTAAGATTGAAAGCGGCAGTTGTTGTTCTAAATAAGTCAATTACTATCCCAAAGGTTGATGGTTCGATCCTCAACCTAAAAAGTTGAAGTTACATCCATATCAACTTCATCCCCATTTCACATCTTGATTTTGCTGACTTTGACTTTGCTCTTGCAGGACCTATGGTGTGCCTTTGCACTTGATCCGAGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAATGATCGCTTTCCTGATGCAACGCCTGAAGAACTCAATTCGTGCGTGCATGCTTGGTCAACTTGTTGTTTCTTTTATGCTTCCAGTACTTTTATATTGGCTACTAAAAATCCTTCTATGGAATTGTAGAAGTGACGCAACATGCATTATCTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCCTGGTTGGAGCGACAACATACTTGCCCAACCTGCAGAGCCCTTGTTGTACCACCTGAAGGTTTAAGCACAGCAGCCGGTCATCATGGACCACAATCTGATGCTAACCAACAAGGTAATTGCACCAATAAGAATTCAATTCATTTCAATTTACTGCCTGGCTAAAGGATCCTACATCTTGTACAATTTTGATAAGTTTATAAATTTCTTCCATGTATATTTCATCTGATTGTGAACATTATTGGAATCTTTCATTTGAGGAAACTGAAACTTCGACTAAGGAAAAGCATGAATGATCCAGAAGGCCTAAGCTAAAAGAGTACTCTTTGATGTTGTTTATGTTTCATTCGGAAACCTAAAGATAGGAAATATGAATCTTCTCAATGTTCTAGGGACAGGAAAATATCTTTTCCACCTGCCTTGTCTCCCACAAAGTAGTCCGTGCAATGCTTTTTTAGTATCTTGTTTGTGCAATGCTTCCCCTGCATCTCTGATTCTTTTTTGCAAGTGGTGCTTTGTATCTGGCATCTAGTAACCCAATTTGGTTTAAAGTTTCAAAACTATTAAATAATCTAGACCATTAATAACCATGCAAAAATAAGCTATCAGTATTTGCAAGTCTTCTCCATATATATACCTCCCTATCAGTGTGAAAGAGTTGAAATTTGGGTCTCTCTCACAATTCGTTTCATGGTTGTTGGCTTTCTCTCTACTCTCTTCTCTGTTATTATTTCTTTTGTATTCATGATTGTTTTTCTTATGAGACTCTCCTTAAAGTTGTTTATTTTATATATTTTTTTATCCTACAACTCAATCTCTTCCAAAGGAAATAGTTTGAAGAACCGGCTATGAACTTCAGCATATCTCTTTGAGAAGGATGATTATTCTTGAACTGGGCCTCTGGTCCTCCGTAAAAATTGGGCGCTTCATTTTTTTCTTGTTTTGTTTTTAACTTTCTTGTTGTGTTAGAAAAGGTGTAGGGGTAATTTAGAAAAGGTGTAGAAGTAATTTCCAAGTTTACATGATCTAATTGCTTTGGATTAAAATGTTTTGTTGTTTGGTGCATTCGTATAGCATTCCCTTCAGTGTATCTGCTTTCTTTAATAGAAGTTTTTTTACTCAAATCATTGAACTGATGGTTTTACTTGATTATTTTTCGATATGGGGTTATACCATGCTGGAACTTGGATACAATGCAATGACTGATCTTTCTGTTGAAAACAGGAACAGGAACCACAAGTTCATCCGCGCAAGGTACGAGTGATACAGGTGTTGCTAATGATAATTTGAGCCATCATCAGGCTAGAGTCCGAGCTGCTGCGGCTGCAGCTTCTGTATATGAGAAATCATATGTTTATCCTTGTGCGAGCACATTAGTATGGTATGCGTTTTTATTGCAAACATCCCACACACGCACCCACCAAAAGGAGAGGAGTATGACAAATTTTTTTAATTATCTTATTTATTTACAGGTCTCCTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGCCTGACTCCAATAACATAGAACCAAATGGACATCCACAACAATTTTCTTTTACCGGCGGCCCTTCCAACCTGTTCTTCACTCAATTTCCCCAGAATCTTTTTGTTCCTTTTCAGCCACGAGAGGGTACTGGAAACAGTACGGACGGATTGGGCAGTAGTCAAAATGTATCAGGCTTACATCTTGAAGCTCAACAAAGATTTCTCCAGCAACAGATTGAGGTATGTTGTTTAATTGTTGCATCAACATTATCTAGTTGGCTCTCATTCTTCTTGATGGAGCCTTTTTCTCTAGTTGGGCTCCCTTTTGTGGTCTTGTTTTTTTGGGTTGCCTTGTATTCTTTCATTTTTTTTTTCTCATTAAAAAATATCATTACCCAATGAACTCTCTTGTTTGGTCAGTGTATGTTTCGTTTGTGCTCTTGTTGCTGGGCCTCTGTACATTCTTTAGCTTAGTTTACTAGTATGCACATGGCCGTGGGTAAATATTGAATTGAGGTCATGACATGTGCTTAGGCACTTGATTCTTAGCGTTGTTTGGTTTTCCCCTTTGTGCTGTTTCCTTGACCTCTGATGTTCAATAACGAGAAGCTAACTTTTTTGCTTTTTATATTCGTGGCTTACCGTTGGGGTGTTTAAAAATGAGCTACCATAAACAGTTGAAAAGAAAAAAAAAAAAAAATTGTGATACTTAGGAGATGTCCTGTTAATATATAAATTTGTATTTATTAGGTAAACCATATGAAATTTATTCGTTCTCTTTTCTTTTCTTTTCTTTCACTGTAAATACGGCTCTTCGTTCAATTCTTTCGAAGTTATTGACAACTTCATTCAAACTAGTCTGAGTATAACAGGGTTGGGTGAGGTTGAGGATTTCTAAATTCAAAATTTCATGTTTTCCAACAGATCCTACAGAATCAGCTGCAACATCTCCGTAAACCAGAAGTTGAGGAAAGCTCAGGAGCAGGTGAGGCAGATAAGAAAGGGAAAAGTGTAGCATTCTCGTCACCGTCGCCATCGCCGTCGTCATCTGATTCTCGATGA

mRNA sequence

ATGTTGATTGGAAAGACCATGTCATCTGAGAATTCATCGGAAAAAGTAGCCGTAGACGCCGATCAATCAGTGCAGTCGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTCATTGCAACCATAGTGGTTATCTATCATGCCTTCAACAGTAGAGGCCAGTTCTACCCAGCACTGGTCTATTTATCGACTTCCAAGATAAATCTAGTGCTTCTTCTCAATATGGGTCTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAGAAAATATTTCTCGGTTCTCTTAGAGAAGCAGAGGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATAACTATTTTCCGCCAAGATTTTTCTGTTTCATTCCTTGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATAGTTTCCTTCTTAGGCTTCCTCTTTGTTCTAGATAGTCTGTTTTTTTATAATTCGATAGAATCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCAACAACGGCTGTGTCAACGTTTGTAAAATATATTTTCTATGTCAGTGATGTACTCATGGAGGGACAATGGGAAAGGAAGCCCGTCTATACATTTTACTTGGAACTTATCCGAGACTTACTTCACTTGTCAATGTACCTGTGTTTCTTTCTTGTGATTTTCATGACCTATGGTGTGCCTTTGCACTTGATCCGAGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAATGATCGCTTTCCTGATGCAACGCCTGAAGAACTCAATTCAAGTGACGCAACATGCATTATCTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCCTGGTTGGAGCGACAACATACTTGCCCAACCTGCAGAGCCCTTGTTGTACCACCTGAAGGTTTAAGCACAGCAGCCGGTCATCATGGACCACAATCTGATGCTAACCAACAAGGAACAGGAACCACAAGTTCATCCGCGCAAGGTACGAGTGATACAGGTGTTGCTAATGATAATTTGAGCCATCATCAGGCTAGAGTCCGAGCTGCTGCGGCTGCAGCTTCTGTATATGAGAAATCATATGTTTATCCTTGTGCGAGCACATTAGTATGGTCTCCTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGCCTGACTCCAATAACATAGAACCAAATGGACATCCACAACAATTTTCTTTTACCGGCGGCCCTTCCAACCTGTTCTTCACTCAATTTCCCCAGAATCTTTTTGTTCCTTTTCAGCCACGAGAGGGTACTGGAAACAGTACGGACGGATTGGGCAGTAGTCAAAATGTATCAGGCTTACATCTTGAAGCTCAACAAAGATTTCTCCAGCAACAGATTGAGATCCTACAGAATCAGCTGCAACATCTCCGTAAACCAGAAGTTGAGGAAAGCTCAGGAGCAGGTGAGGCAGATAAGAAAGGGAAAAGTGTAGCATTCTCGTCACCGTCGCCATCGCCGTCGTCATCTGATTCTCGATGA

Coding sequence (CDS)

ATGTTGATTGGAAAGACCATGTCATCTGAGAATTCATCGGAAAAAGTAGCCGTAGACGCCGATCAATCAGTGCAGTCGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTCATTGCAACCATAGTGGTTATCTATCATGCCTTCAACAGTAGAGGCCAGTTCTACCCAGCACTGGTCTATTTATCGACTTCCAAGATAAATCTAGTGCTTCTTCTCAATATGGGTCTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAGAAAATATTTCTCGGTTCTCTTAGAGAAGCAGAGGTTGAGAGGCTCAACGAGCAATCATGGAAGGAGTTGATGGAAATCCTCTTTGCCATAACTATTTTCCGCCAAGATTTTTCTGTTTCATTCCTTGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATAGTTTCCTTCTTAGGCTTCCTCTTTGTTCTAGATAGTCTGTTTTTTTATAATTCGATAGAATCTCTGATACAGACAAGGAAGGCCTCGGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCAACAACGGCTGTGTCAACGTTTGTAAAATATATTTTCTATGTCAGTGATGTACTCATGGAGGGACAATGGGAAAGGAAGCCCGTCTATACATTTTACTTGGAACTTATCCGAGACTTACTTCACTTGTCAATGTACCTGTGTTTCTTTCTTGTGATTTTCATGACCTATGGTGTGCCTTTGCACTTGATCCGAGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAATGATCGCTTTCCTGATGCAACGCCTGAAGAACTCAATTCAAGTGACGCAACATGCATTATCTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCCTGGTTGGAGCGACAACATACTTGCCCAACCTGCAGAGCCCTTGTTGTACCACCTGAAGGTTTAAGCACAGCAGCCGGTCATCATGGACCACAATCTGATGCTAACCAACAAGGAACAGGAACCACAAGTTCATCCGCGCAAGGTACGAGTGATACAGGTGTTGCTAATGATAATTTGAGCCATCATCAGGCTAGAGTCCGAGCTGCTGCGGCTGCAGCTTCTGTATATGAGAAATCATATGTTTATCCTTGTGCGAGCACATTAGTATGGTCTCCTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGCCTGACTCCAATAACATAGAACCAAATGGACATCCACAACAATTTTCTTTTACCGGCGGCCCTTCCAACCTGTTCTTCACTCAATTTCCCCAGAATCTTTTTGTTCCTTTTCAGCCACGAGAGGGTACTGGAAACAGTACGGACGGATTGGGCAGTAGTCAAAATGTATCAGGCTTACATCTTGAAGCTCAACAAAGATTTCTCCAGCAACAGATTGAGATCCTACAGAATCAGCTGCAACATCTCCGTAAACCAGAAGTTGAGGAAAGCTCAGGAGCAGGTGAGGCAGATAAGAAAGGGAAAAGTGTAGCATTCTCGTCACCGTCGCCATCGCCGTCGTCATCTGATTCTCGATGA

Protein sequence

MLIGKTMSSENSSEKVAVDADQSVQSRMMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTTSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR
Homology
BLAST of CmoCh16G002830 vs. ExPASy Swiss-Prot
Match: Q6NPT7 (ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=3702 GN=HRD1B PE=2 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 6.9e-176
Identity = 337/528 (63.83%), Postives = 397/528 (75.19%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GLSTAAGHHGPQSDANQQGTGT 387
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E   STA+G+ GP  ++ QQGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 388 TSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYP-CASTLVWSPGYAVVPQV 447
           +SS  QG+S +  A++N+S H+AR +AAA+AAS+Y +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 420

Query: 448 ERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNV 507
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 457

Query: 508 SGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVA 554
               +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 481 ----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 457

BLAST of CmoCh16G002830 vs. ExPASy Swiss-Prot
Match: Q9LW77 (ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=3702 GN=HRD1A PE=2 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.3e-174
Identity = 343/535 (64.11%), Postives = 401/535 (74.95%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEY+ETTPSV  LSH RIVSF+GFL ++DSLF Y+SI  LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE  ++AA         +QQ  GT+
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ--GTS 360

Query: 388 SSSAQG---TSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQ 447
           SS  QG   +S  GV+N++LS H AR++AAA+AASVY KS VYP A+T+ WS G     Q
Sbjct: 361 SSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSGVPGTEQ 420

Query: 448 V----ERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLG 507
           V    ++ LP  N    N H                                        
Sbjct: 421 VSTEPDQTLPQHNLPVENSH---------------------------------------- 480

Query: 508 SSQNVSGLHLEAQQRFLQQQIEILQNQLQHL--RKPEVEESSGAGEADKKGKSVA 554
           +  N+S   LE  ++ L+  +EIL+N+L  L  RKPE      AGE + KGKSVA
Sbjct: 481 AYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPE-----SAGEPENKGKSVA 488

BLAST of CmoCh16G002830 vs. ExPASy Swiss-Prot
Match: Q5Z880 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD1 PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 3.5e-159
Identity = 306/503 (60.83%), Postives = 370/503 (73.56%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M++LQTYA  S +AT   +Y+AF+SR QFYPA+VYLSTSKI  VLLLN GLV MC+ WQL
Sbjct: 1   MIRLQTYAAFSLMATATAVYYAFSSREQFYPAMVYLSTSKICFVLLLNTGLVAMCVAWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
            K++FLG+LREAEVERLNEQ+W+E++EILFA+TIFRQDFSVSFL MV ALLL+KALHWLA
Sbjct: 61  VKRLFLGTLREAEVERLNEQAWREVVEILFAVTIFRQDFSVSFLAMVAALLLVKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEY+ETTPSVP+LSH RIVSF+ FL V+D LF  NS+ SLI  R+ASV++FFSFEYM
Sbjct: 121 QKRVEYIETTPSVPMLSHARIVSFMLFLLVVDCLFLSNSLRSLIHKREASVAIFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILAT+ VSTFVKYIFYVSD+LMEGQWE+K VYTFYLELI DL+HLS+Y+ FF+ IF+ YG
Sbjct: 181 ILATSTVSTFVKYIFYVSDMLMEGQWEKKAVYTFYLELISDLVHLSLYMLFFIAIFLNYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYE FRNFRIRIADY+RYRKITSNMN+RFPDAT +ELN+SDATCIICREEMT
Sbjct: 241 VPLHLIRELYETFRNFRIRIADYVRYRKITSNMNERFPDATADELNASDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQ-QGTGT 387
            AKKL+CGHLFHVHCLRSWLERQHTCPTCRA ++PP+   TAA  HG         G GT
Sbjct: 301 TAKKLLCGHLFHVHCLRSWLERQHTCPTCRAPILPPDNGRTAARPHGVHPGVQPVPGNGT 360

Query: 388 TSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVE 447
             S          A +N+S  QA++ AAA+AAS+Y +S+ YP A+ L     Y+  PQ  
Sbjct: 361 PGSER-------AAGENISRRQAKLEAAASAASLYGRSFAYPPANNL---NRYSTPPQST 420

Query: 448 RPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVS 507
              P S     +    +   T  PS                P      +   + +++ + 
Sbjct: 421 SNGPQSGEASTSNQSPKGHATADPS---------------APTFYARGAVSSVTTTRELE 478

Query: 508 GLHLEAQQRFLQQQIEILQNQLQ 530
               +A +  ++ QIE+LQ QLQ
Sbjct: 481 SSLQKAYENAIKSQIEMLQIQLQ 478

BLAST of CmoCh16G002830 vs. ExPASy Swiss-Prot
Match: Q803I8 (E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2)

HSP 1 Score: 264.6 bits (675), Expect = 2.5e-69
Identity = 141/331 (42.60%), Postives = 201/331 (60.73%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M++       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L +L
Sbjct: 1   MVRAALVTATSLALTGAVVAHAYFLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
            +K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MRKVFFGQLRAAEMEHLIERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           + RV++ME +P++  + H R++S +  L V+D LF  ++  S+I TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWVFHFRVLSLMVLLGVMDFLFVNHACHSII-TRGASVQLVFGFEYA 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           IL T  ++TF+KY  +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTTFIKYTLHTIDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 327
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPE+L ++D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEDLQATDNVCIICREEMV 300

Query: 328 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 358
           T AKKL C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKKLPCNHIFHSSCLRSWFQRQQTCPTCR 330

BLAST of CmoCh16G002830 vs. ExPASy Swiss-Prot
Match: Q86TM6 (E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-68
Identity = 141/331 (42.60%), Postives = 198/331 (59.82%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M +       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L ++
Sbjct: 1   MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKV 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
             K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           + RV++ME +P++  L H RIVS +  L +LD LF  ++  S++ TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSIL-TRGASVQLVFGFEYA 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           IL T  ++ F+KY+ +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 327
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPEEL + D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMV 300

Query: 328 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 358
           T AK+L C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKRLPCNHIFHTSCLRSWFQRQQTCPTCR 330

BLAST of CmoCh16G002830 vs. ExPASy TrEMBL
Match: A0A6J1E3K4 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=3662 GN=LOC111430487 PE=4 SV=1)

HSP 1 Score: 1039.3 bits (2686), Expect = 6.1e-300
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 448 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 507
           PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 480

Query: 508 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR 567
           LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR
Sbjct: 481 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR 540

BLAST of CmoCh16G002830 vs. ExPASy TrEMBL
Match: A0A6J1J826 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=3661 GN=LOC111482600 PE=4 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 5.9e-295
Identity = 534/543 (98.34%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGL+IMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLIIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDAT EELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATSEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG STAAGHHGPQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 448 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 507
           PLPDSNNIEPNGHPQQFSFTGGPSNL FTQFPQNLFVPFQPREGTGN+TDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNNTDGLGSSQNVSG 480

Query: 508 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAF---SSPSPSPSSS 567
           LHLEAQ+RFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAF   SSPSPSPSSS
Sbjct: 481 LHLEAQERFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSLSSPSPSPSSS 540

BLAST of CmoCh16G002830 vs. ExPASy TrEMBL
Match: A0A5A7V9K3 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001010 PE=4 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.2e-271
Identity = 503/547 (91.96%), Postives = 516/547 (94.33%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG ST +GHHG QSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTS TGV NDNLSHHQARVRAAAAAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 448 PLPDSNNIE------PNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGS 507
           PL DSNN E      PNGHPQ FSF GGPSNL FTQFPQN+FVPFQPREGTGN+ + LGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 508 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESS-GAGEADKKGKSVAFS-SPSP 567
           S NVSG  LEAQ++FLQQQIE LQNQLQ LRKP+VEESS GA E DKKGKS+A S +PS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPAPSA 540

BLAST of CmoCh16G002830 vs. ExPASy TrEMBL
Match: A0A0A0LJG4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.0e-271
Identity = 504/547 (92.14%), Postives = 515/547 (94.15%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG ST  GHHG QSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTS TGV NDNLSHHQARVRAAAAAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 448 PLPDSNNIE------PNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGS 507
           PL DSNN E      PNGHPQ FSF GGPSNL FTQFPQN+FVPFQPREGTGN+ + LGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 508 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESS-GAGEADKKGKSVAFS-SPSP 567
           S NVSG  LEAQ++FLQQQIE LQNQLQ LRKP+VEESS GA EADKKGKS+A S S S 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540

BLAST of CmoCh16G002830 vs. ExPASy TrEMBL
Match: A0A1S4E231 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 GN=LOC103495570 PE=4 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 4.5e-271
Identity = 503/547 (91.96%), Postives = 515/547 (94.15%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG ST +GHHG QSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTS TGV N NLSHHQARVRAAAAAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNANLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 448 PLPDSNNIE------PNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGS 507
           PL DSNN E      PNGHPQ FSF GGPSNL FTQFPQN+FVPFQPREGTGN+ + LGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 508 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESS-GAGEADKKGKSVAFS-SPSP 567
           S NVSG  LEAQ++FLQQQIE LQNQLQ LRKP+VEESS GA E DKKGKS+A S SPS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPSPSA 540

BLAST of CmoCh16G002830 vs. NCBI nr
Match: KAG6576867.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.6e-300
Identity = 541/545 (99.27%), Postives = 543/545 (99.63%), Query Frame = 0

Query: 23  SVQSRMMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMC 82
           +VQSRMMKLQTYAGISFIAT+VVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMC
Sbjct: 2   AVQSRMMKLQTYAGISFIATVVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMC 61

Query: 83  ILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKA 142
           ILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKA
Sbjct: 62  ILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKA 121

Query: 143 LHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFF 202
           LHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFF
Sbjct: 122 LHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFF 181

Query: 203 SFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVI 262
           SFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVI
Sbjct: 182 SFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVI 241

Query: 263 FMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIIC 322
           FMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIIC
Sbjct: 242 FMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIIC 301

Query: 323 REEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQ 382
           REEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG STAAGHHGPQSDANQQ
Sbjct: 302 REEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQ 361

Query: 383 GTGTTSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVV 442
           GTGTTSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVV
Sbjct: 362 GTGTTSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVV 421

Query: 443 PQVERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSS 502
           PQVERPLPDSNNIEPNGHPQQFSFTGGPSNL FTQFPQNLFVPFQPREGTGNSTDGLGSS
Sbjct: 422 PQVERPLPDSNNIEPNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNSTDGLGSS 481

Query: 503 QNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPS 562
           QNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPS
Sbjct: 482 QNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPS 541

Query: 563 SSDSR 568
           SSDSR
Sbjct: 542 SSDSR 546

BLAST of CmoCh16G002830 vs. NCBI nr
Match: XP_022922503.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_022922504.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata])

HSP 1 Score: 1039.3 bits (2686), Expect = 1.3e-299
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 448 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 507
           PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 480

Query: 508 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR 567
           LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR
Sbjct: 481 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR 540

BLAST of CmoCh16G002830 vs. NCBI nr
Match: XP_022984215.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita maxima] >XP_022984216.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita maxima])

HSP 1 Score: 1022.7 bits (2643), Expect = 1.2e-294
Identity = 534/543 (98.34%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGL+IMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLIIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDAT EELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATSEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG STAAGHHGPQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 448 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 507
           PLPDSNNIEPNGHPQQFSFTGGPSNL FTQFPQNLFVPFQPREGTGN+TDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNNTDGLGSSQNVSG 480

Query: 508 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAF---SSPSPSPSSS 567
           LHLEAQ+RFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAF   SSPSPSPSSS
Sbjct: 481 LHLEAQERFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSLSSPSPSPSSS 540

BLAST of CmoCh16G002830 vs. NCBI nr
Match: XP_023553481.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo] >XP_023553482.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1014.2 bits (2621), Expect = 4.3e-292
Identity = 531/540 (98.33%), Postives = 532/540 (98.52%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG STAAGHHGPQSDANQQG GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGAGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTSDTGVANDNLSHHQARVRAA AAASVYEKSYVYPCASTLVWSPGYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAVAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 448 PLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNVSG 507
           PLPDSNNIEPNG PQQFSFTGGPSNL FTQFPQNLFVPFQPRE TGNSTDGLGSSQNVSG
Sbjct: 421 PLPDSNNIEPNGRPQQFSFTGGPSNLSFTQFPQNLFVPFQPREDTGNSTDGLGSSQNVSG 480

Query: 508 LHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVAFSSPSPSPSSSDSR 567
           LHLEAQQRFLQQQIEILQNQLQHLRKP+VEESSGAGEADKKGKSVAFS  SPSPSSSDSR
Sbjct: 481 LHLEAQQRFLQQQIEILQNQLQHLRKPDVEESSGAGEADKKGKSVAFSL-SPSPSSSDSR 539

BLAST of CmoCh16G002830 vs. NCBI nr
Match: XP_038889876.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_038889877.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida])

HSP 1 Score: 950.7 bits (2456), Expect = 5.9e-273
Identity = 507/546 (92.86%), Postives = 516/546 (94.51%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEG STA GHHG QSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHGVQSDANQQGTGTT 360

Query: 388 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 447
           SSSAQGTS TGV NDNLSHHQARVRAAAAAAS+YEKSYVYPC STLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCPSTLVWSLGYAVVPQVER 420

Query: 448 PLPDSNNIE------PNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGS 507
           PLPDS+N E      PNGHPQ FSF  GPSNL FTQFPQNLFVPFQPREGTGN+TD LGS
Sbjct: 421 PLPDSDNTETNGEQTPNGHPQYFSFPSGPSNLSFTQFPQNLFVPFQPREGTGNNTDELGS 480

Query: 508 SQNVSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVA-FSSPSPS 567
           S NVSG  LEAQ++FL QQIEILQNQLQ LR+P+VEESS AGEADKKGKS+A  SSP PS
Sbjct: 481 SLNVSGSQLEAQEKFL-QQIEILQNQLQLLRRPKVEESSRAGEADKKGKSLATSSSPLPS 540

BLAST of CmoCh16G002830 vs. TAIR 10
Match: AT1G65040.2 (RING/U-box superfamily protein )

HSP 1 Score: 618.6 bits (1594), Expect = 4.9e-177
Identity = 337/528 (63.83%), Postives = 397/528 (75.19%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GLSTAAGHHGPQSDANQQGTGT 387
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E   STA+G+ GP  ++ QQGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 388 TSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYP-CASTLVWSPGYAVVPQV 447
           +SS  QG+S +  A++N+S H+AR +AAA+AAS+Y +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 420

Query: 448 ERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNV 507
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 457

Query: 508 SGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVA 554
               +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 481 ----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 457

BLAST of CmoCh16G002830 vs. TAIR 10
Match: AT3G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 614.4 bits (1583), Expect = 9.3e-176
Identity = 343/535 (64.11%), Postives = 401/535 (74.95%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEY+ETTPSV  LSH RIVSF+GFL ++DSLF Y+SI  LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGLSTAAGHHGPQSDANQQGTGTT 387
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE  ++AA         +QQ  GT+
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ--GTS 360

Query: 388 SSSAQG---TSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQ 447
           SS  QG   +S  GV+N++LS H AR++AAA+AASVY KS VYP A+T+ WS G     Q
Sbjct: 361 SSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSGVPGTEQ 420

Query: 448 V----ERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLG 507
           V    ++ LP  N    N H                                        
Sbjct: 421 VSTEPDQTLPQHNLPVENSH---------------------------------------- 480

Query: 508 SSQNVSGLHLEAQQRFLQQQIEILQNQLQHL--RKPEVEESSGAGEADKKGKSVA 554
           +  N+S   LE  ++ L+  +EIL+N+L  L  RKPE      AGE + KGKSVA
Sbjct: 481 AYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPE-----SAGEPENKGKSVA 488

BLAST of CmoCh16G002830 vs. TAIR 10
Match: AT1G65040.3 (RING/U-box superfamily protein )

HSP 1 Score: 486.1 bits (1250), Expect = 3.8e-137
Identity = 282/528 (53.41%), Postives = 335/528 (63.45%), Query Frame = 0

Query: 28  MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 87
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINL                 
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINL----------------- 60

Query: 88  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 147
                                                                  LHW+A
Sbjct: 61  ------------------------------------------------------GLHWMA 120

Query: 148 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 207
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 208 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 267
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 268 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 327
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 328 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GLSTAAGHHGPQSDANQQGTGT 387
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E   STA+G+ GP  ++ QQGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 388 TSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYP-CASTLVWSPGYAVVPQV 447
           +SS  QG+S +  A++N+S H+AR +AAA+AAS+Y +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 386

Query: 448 ERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQNV 507
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 386

Query: 508 SGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVA 554
               +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 481 ----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 386

BLAST of CmoCh16G002830 vs. TAIR 10
Match: AT1G65040.1 (RING/U-box superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 1.4e-99
Identity = 203/349 (58.17%), Postives = 238/349 (68.19%), Query Frame = 0

Query: 207 MILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTY 266
           MILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM Y
Sbjct: 1   MILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNY 60

Query: 267 GVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM 326
           G+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEM
Sbjct: 61  GLPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEM 120

Query: 327 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GLSTAAGHHGPQSDANQQGTG 386
           T AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E   STA+G+ GP  ++ QQGTG
Sbjct: 121 TSAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTG 180

Query: 387 TTSSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYP-CASTLVWSPGYAVVPQ 446
           T+SS  QG+S +  A++N+S H+AR +AAA+AAS+Y +S VYP  A+TLVWS G +++PQ
Sbjct: 181 TSSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQ 240

Query: 447 VERPLPDSNNIEPNGHPQQFSFTGGPSNLFFTQFPQNLFVPFQPREGTGNSTDGLGSSQN 506
            E                                                          
Sbjct: 241 TE---------------------------------------------------------- 278

Query: 507 VSGLHLEAQQRFLQQQIEILQNQLQHLRKPEVEESSGAGEADKKGKSVA 554
                +EAQ+RFL+ QIE+L NQL+ L KP           D KGKSVA
Sbjct: 301 -----VEAQRRFLESQIEVLTNQLRLLEKPTT--------VDTKGKSVA 278

BLAST of CmoCh16G002830 vs. TAIR 10
Match: AT4G25230.1 (RPM1 interacting protein 2 )

HSP 1 Score: 94.7 bits (234), Expect = 2.5e-19
Identity = 97/343 (28.28%), Postives = 153/343 (44.61%), Query Frame = 0

Query: 52  SRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKE 111
           S G    AL  L  S   + LL N  L +  +L    K +F G L + E ++L E+    
Sbjct: 42  SLGDSEHALELLLGSYFTIALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANY 101

Query: 112 L------MEILFAITIFRQDFSVSFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSH 171
           +      + ++   TIF+    V +   +T L  +K    LA+ R+E +  +PS    ++
Sbjct: 102 IIYKGTFLPLVIPPTIFQ---GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTY 161

Query: 172 IRIVSFLGFLFVLD------SLFFYNSIESLIQTRKASVSLFFSFE----YMILATTAVS 231
            R+ S L  +  +D      SL  YN+I S +           +FE     +I     + 
Sbjct: 162 FRVYSVLFLVLSVDMFWIKLSLMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLD 221

Query: 232 TFVKYIF----------YVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMT 291
            ++ ++           ++  +      E K +    L    D+  L M L  +L I+  
Sbjct: 222 MWINHLAVKNSDCQRSKFIDSMTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWL 281

Query: 292 YGVPLHLIRELYEIFRNFRI-------RIADYIRYRKITSNMNDRFPDATPEELNSSDAT 351
           +G+  HL+  +  +F N R        RI  YI+ R     ++   PDAT EEL + D  
Sbjct: 282 HGIAFHLVDAV--LFLNIRALLSAILKRIKGYIKLRIALGALHAALPDATSEELRAYDDE 341

Query: 352 CIICREEMTVAKKLVCGHLFHVHCLRSWLER----QHTCPTCR 358
           C ICRE M  AK+L C HLFH+ CLRSWL++     ++CPTCR
Sbjct: 342 CAICREPMAKAKRLHCNHLFHLGCLRSWLDQGLNEVYSCPTCR 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NPT76.9e-17663.83ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=370... [more]
Q9LW771.3e-17464.11ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=370... [more]
Q5Z8803.5e-15960.83ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica... [more]
Q803I82.5e-6942.60E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2[more]
Q86TM61.3e-6842.60E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1E3K46.1e-300100.00ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=... [more]
A0A6J1J8265.9e-29598.34ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=36... [more]
A0A5A7V9K31.2e-27191.96ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. maku... [more]
A0A0A0LJG42.0e-27192.14RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 P... [more]
A0A1S4E2314.5e-27191.96ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
KAG6576867.15.6e-30099.27ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosper... [more]
XP_022922503.11.3e-299100.00ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_... [more]
XP_022984215.11.2e-29498.34ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita maxima] >XP_02... [more]
XP_023553481.14.3e-29298.33ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pe... [more]
XP_038889876.15.9e-27392.86ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_0... [more]
Match NameE-valueIdentityDescription
AT1G65040.24.9e-17763.83RING/U-box superfamily protein [more]
AT3G16090.19.3e-17664.11RING/U-box superfamily protein [more]
AT1G65040.33.8e-13753.41RING/U-box superfamily protein [more]
AT1G65040.11.4e-9958.17RING/U-box superfamily protein [more]
AT4G25230.12.5e-1928.28RPM1 interacting protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 319..356
e-value: 2.0E-5
score: 34.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 318..357
e-value: 1.2E-10
score: 41.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 319..357
score: 12.415513
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 291..362
e-value: 6.8E-23
score: 81.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 373..401
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 553..567
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 532..567
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..401
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 28..475
NoneNo IPR availablePANTHERPTHR22763:SF174SUBFAMILY NOT NAMEDcoord: 28..475
NoneNo IPR availableCDDcd16479RING-H2_synoviolincoord: 316..357
e-value: 1.16974E-27
score: 103.147
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 299..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G002830.1CmoCh16G002830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity