CmoCh16G002670 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G002670
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRibonuclease T(2)
LocationCmo_Chr16: 1191523 .. 1197221 (+)
RNA-Seq ExpressionCmoCh16G002670
SyntenyCmoCh16G002670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTTCTCCATTAAATCCAACCCCATGGTGGTGGCGCCGGCGGTGTTTCTTCTTGTTCTTCAACTGGTGGCGGTGGTACTCGCGGCGGAGACTCCAAGCGGGGGCGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGCTACTTCTTGCAAGAACACTGGAAAGTGTTGCCCGGCCAATGGTTGCTGCCGTGGGTGAGCTCTTGTCTCCGACATTAAGCTTGATCCAGACGTTGACTTTTGAAAGCTGACTTTTTTTTGTGTGTTTTCTCTGTGTTTGAATATTTTGTTTGTCAACTGTCTAGAGCTGACTCTCCCACTGAATTTACAATCCGTAAGTGGAAAACTAAGCCCTCTAAGGTTTGGAATTTGGATGTTTTGTGTATTAAGATCTGAAAAGTTTGTGTTTTGAATGTGTCAGATGGGCTATGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAGTCTTCAGTCAAAATGAGGTCTCTCTCTCTCTCTTTCTCTCTCTCGAACTCGAATGAGTATAACAAATGATTCTACGATTGTTTGCTATGTTAAACGGTCTAGGTCCACCGTTAGTAGATATTGTCCACTTTGACCCATTACGTATCACTGTTACCTCACAGTTTTAAAACGCGTCTACTAGGGAGAGGTTTTCACACCCTTATAAATAATTATTCATTCTCCTCTCCAACTAATGTGCAATCTCACAATCCATCTCTTTTGGGGGCCCAACGTCCTCGTTGGCGGTGATTCCATTTGTAACCGTTCAAACTCACCGCTAGCAAATATTGTTTTCTTTAAATTTTTCCTTCTAGGCTTCTCCTCAAGATTTTAAAATGTGTCTGTTAGGGAGAGATTTCCACACCCTTATAAGAAATGTTTTATTCTCCTCTCCAACCAATGTAGGATCTCACAATCCACCCCCTTGAAGACCCAACGTCTTCGCTGGCATACCGCTCGATGTCTAACTCTGATACTATTTGTAACAACCCAAGCCCACTACTAGCAGATATTGTCGTGTTTGAATTTTTCCTTCTGGACTTCCTCTTAAAGTTTTAAAACACGTAGGTTAGGAAAAGGTTTTCATACCCTTCTAAGGAATGCAATCTCGAAACTAACACAATTTTCTCTTACCCGTTACTAAACACAATTGCTTGAGTTCAACAATTATGTACAACAAATCAAACTCTTAATATTTAGAATGATAATTAGTGTCTTACCCATTTGCAGATTACCTTGTTGAAGGAGGATATTGAAAAGTATTGGCCAACCTATAGATGTGGTACTACATCCAACTGCCATGGTACCAAAGGATCATTTTGGGCTCATGAGGTTCATATCACTCTCTCTTTGTTCTTCCTTAGCTCATCTGTCAAACTTTGAGACTAATCCAAAATTCCTTTCCTTTTTCTCTTCTTCTCATATGATCGCCATTATCATATCAAATGCTACCATTGCTGCAGTATGGTCAGTTTCTTCTTCACTTCCAATCTTCTTGGAACTTGATATCTAGTTCTACTCAAATTCTAACCCAAACCCTCCATTTTCAGAGAAGCATGGGACCTGCGCAGCTCCTGTGATTGTCGGCGAATTCGATTACTTCTTGACAACGCTCACCATCTTCTCCAAGTACAATGTCACGGTAAGTAATACATCTCTATTGATATGAGGCCTTTTGGGGAAGCTCAAAGCAAAGCCACGAGAGCTTATGCTCAAAATGGACAATATCATACTATTATGGAGAGTCGTGATTACTAACATAGTATTAGAGTCATGCCTTAACTTAATCATGTCAATAGAATACTCAAATGTCGAACAAAGAAGTTGTGAGCCTCGAATGTGTAGTCAAAAGTGACTCAAGTGTCGAACAAATGGTGTACTTTGTTTGATGACTCCAGAGAAAGAGTCGAACCTCGATTAAGGGGAGGTTGTTCGAGGGCTTCATAGGCTTCAGGGGAGGCTCTATAGTGTACTTTGGTCGAAATACATCTTCGCTGGTATAAGGCCTTTTGAAAGTCAGCCATATGGTTTTGGTTGTGAGTATTAAATTTGGTGTCTTTTTCATTGTGGGTATCAAATTTGGTGTCTTTTTCGTTGTGGGTATCAAATTTGGTGTCATTTTCGTTGTGGGTATTAAATTTGGTGTCTTTTTCGTTATGGGTATTAAATTTGGTGTCTTTTTCAATTTGTTTATACCAGAAAGCGCTGAGCGATGCTGGGTTCGTAGCTTCCAATACTGAGAAGTATCCACTAGAAGATGTGGTGGATGCAGTCAAGAATGCTACCAATTCAACTCCAAAGATTAGCTGTGCTAAAAAGGGGGCTGTCAAGGAGCTTTGGATATGCTTTGACAAGAAATTTGAGGTGTTCTTTGTGTTCATAACAATCATATCTAATAAGCTTTTGGGTTGGATCAAATTGTTCATAGTTTTTGGTTGTTGCAGCCACGTGACTGTGATCTTGCCAATTCATGCCCCGAGTTTATTAAGCTCCCAACGTATGAGCCTCAGGATGTGGTGGAGGGAGCTATTCCATGGATTGCTGATGCTGAAGAACTTATTTAACCTGTTTTTGAAGCTCAAGTTGGACGATCCTTCTTGATGTCTAATTATCACTCTTTGAACTATAAAAGAGGGAGATCTCTCCTTTCTTTTTGTAGTTTCAATGCTATGAACTTAACTTTTTCTTTTTCTAAGTCATATTCCATATAAGATATGGTCTATTACTTACCTTTCTTGCCTTCAATTATGTTCTTTTACTATTCCAAAAGAAAATTAATGTTCTTTTCAACGACCCGAGTAAGATATGACTGAAGTAAGACCATACTCGAATAAGAGTAATCTTGTAAAAACTCGTTACGTATCGTCGTTAGTCTCACGGTTTAAACAAGTCTTTCAGAGTTAGACGGCGAAATGAGTACTCTTCCAAATACTGCCCTTTTTTGCTTTCTCTTCTTGACTTCTTCTTGGTGTTTTTGGGTTTTCCCTTCTGGGCTTCCCCATTACGTGTCTCTGTTATACTCATCCAGATACTGTTCTCTTTGGACTTTCTCTTTCCGACTTTCCCTCGAGGTTTTTGAGTTTTTCCTTTTAAGGTTCTCCATTACGAGTCTATTAGGGGGAGGTTTACATACCCTTATAAGGAAAGCTTCGTTTCCATCGGCTTAAAAAAAATGAAAGTTACTACCTATACTAATAAAAGATAACGATGCGTGCTCTTTAATCAAAAGTGGACATTATTACTCCGAGATATATATAGAAATATAAAAAGGTCACTCATTCCAATAAATTGTATTACTACATTTGAATTATTAAAGGTAGAGGGAAAAAAATCCATTATTTATTTATTTATTTATTTTACTAAATATTTTTATTGAAAACATAAAATAAAATGACATTTTTGCCCATCAAAACTACCAAAATTACCTTAACCTTGTATAAGTAATGATTTGTAGAAAGAATAATAATAATAACATGGCTTCTTTCACGTTCAAAGCGGCGGCGCAGGTGGTGCTTATGGTTCTTGCTGTTACTCTTCAGCCGCAGGCGGCGGAGGGCCTCACCGTCGGCAAGGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGGCTCTTCCTGCAGAAACGTAAGCATGTGCTGCCCCACCAATGGTTGCTGCCACCGGTGAGAGTTTCATCCATATTCTCGTGGTACCTGAAATTCCGATCGTGTCGTGCCTTTTATTATCGAGCTAATTACGTTCTTGTTGTGCATGTCTCTCTAGAGCTGAATTTCCCGCAGAGTTTACAATCCGTAAGTGTGAAAGAAAAGAAGATTTTGCTATTGATTTGGATGTGATGTTCAATAATGATATTATAAAAGTTCGGGTTTGGTAAAAAAAATCTCGTAACCCAACCCAACTCGAGCCATGTACACCCCTAATATAAGTGTTTGAAAATGGCGCATGCAGATGGGCTATGGCCTCAATATCAAGAGAAAGGGTGGCCTTCATGTTGTACTAATGAAACCTTCAACGAAAATGAGGTTATTTCCTCTTACTCTCTCTAAAAATTAGGGTTTGTTATACGAAAATATTGACAAACCTGTGAACCGTACTATTTCTTTAAACCTTGTAGGTTAGTTCCTTGTTTGAAGATCTTGACCGTTACTGGCCAAGCTATCGGTGTGGTCTCGTGAGCAGCTGCGATAACAAAAAAGGATCATTTTGGGCTCACCAGGTTCAAATTCTCGAGTGAAAAATATAGTTTTGGTTCACGAAGTTTGACTTAGGGCTATGAGTTTTCAAACTCGATGAATCACGACGACCCATCTTAAAATTAAAATGTCGAGTTTGAAAATTCAGATAGTTAAATACTACTTAAGTCAAACTTCAAGAGTCAAAAGTATATCTTTCTCGAGTCTCAATCTCTCGGTTATTTCTTCCTTAGTTGATTATGAAAATTCCGTGACTGAATCCCGAAATTCTCTCTTTTTCTCGGGTTATTATATGATGGGTCATATGACATGTTACGTTTGATGCAGTATGGTATGTTTTTTTCTCCTTTAAAAAATATATTTTTGGGAGGAAAAAAAACACATTTAAAATACAATTTATTTTTGTGCAGAGAAGCATGGAACCTGTGGATCACCTGTAATCCTCCACCAATATGATTACTTCTTCACAACTCTCGCCCTTTTCTTTAACTACAATGTCACTGTAAGCTCTTATAAACCTCTCTCAAATGGATGTTTGTGTGCCTTCTTTTCGATGCATAGCTCGGGTAAGAATTCGGTGAAACAAACGAGAAATTATTGTAAGAATTCAGTCACGTTTCCCACAAAAAAAATAAATGGAGAATTTTGATGATTAAAATAGGAGGTGGGAACGAGGATAGTAAAGGCTTTCTTGTTGTCTTTTATCTTCTACAGTTATCGATTTTCGACCGTTAAAATCTCAGGAAGTTTTGGAGGATGCTGAAATTGTAGCTTCTGATACCAAAAAGTATGGTATTGAAGATGTGTTTGAAGCTGTTCGGTCTGCATTTAAAGCGAATCCAAAGCTTAGCTGCGGTAAAAACGGGATTATTAAGGAACTTCGTCTATGCTTTGATAAAGAATTCAAGGTAATACATCACTTTGTGTCTATAAAAGACCTTAGCTACTAGTTTATAAAAGACCTTAGCTACTAGTTTCCATCCAATGAGGAACGTAAATCATTTACTCGTTGCAGGTAATCGATTGTGATGCTACAAAATCATGTCCCAAATTTGTTCGTCTACCAGAATTCCATGAACTACCAAGTAAGTTTCTTATATCTCTTTAAGTGTAGAGATCACATATATATTATAGTTAAGACTAAATAGAACACCGATATTAAAATTGAAAAGATTTGGACGAGGATAACACACCTAACTCCGACAAATTATTTCATTAAAGATCAATATTTAAATTAATAATGAATATATTTATGTATTTGAGGTTGCTTTTGATAAAATTATATTGTGTGTATTGCAGGGTCGACCATGGTTAAGAGAGCAATTCCAAGAATGATTGACGTTGAAGCAACTTCATTTTTTTCGTCAAACAAAGTGTGAAATTCAAGAGTATTTGAATTAATTTAGTGAAATAGTTAACTTAGTTTGTCGAATAACAATTCACAAAAGTTTCCATCTTTGTC

mRNA sequence

ATGGCTTCTTTCTCCATTAAATCCAACCCCATGGTGGTGGCGCCGGCGGTGTTTCTTCTTGTTCTTCAACTGGTGGCGGTGGTACTCGCGGCGGAGACTCCAAGCGGGGGCGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGCTACTTCTTGCAAGAACACTGGAAAGTGTTGCCCGGCCAATGGTTGCTGCCGTGGAGCTGACTCTCCCACTGAATTTACAATCCATGGGCTATGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAGTCTTCAGTCAAAATGAGGATCATTTTGGGCTCATGAGGTTCATATCACTCTCTCTTTGTTCTTCCTTAGCTCATCTGTCAAACTTTGAGACTAATCCAAAATTCCTTTCCTTTTTCTCTTCTTCTCATATGATCGCCATTATCATATCAAATGCTACCATTGCTGCAGTATGGTCAAAAGCGCTGAGCGATGCTGGGTTCGTAGCTTCCAATACTGAGAAGTATCCACTAGAAGATGTGGTGGATGCAGTCAAGAATGCTACCAATTCAACTCCAAAGATTAGCTGTGCTAAAAAGGGGGCTGTCAAGGAGCTTTGGATATGCTTTGACAAGAAATTTGAGGTGTTCTTTGTGTTCATAACAATCATATCTAATAAGCTTTTGGGTTGGATCAAATTGTTCATAGTTTTTGGTTGTTGCAGCCACGTGACTGTGATCTTGCCAATTCATGCCCCGAGTTTATTAAGCTCCCAACGTATGAGCCTCAGGATGTGGTGGAGGGAGCTATTCCATGGATTGCTGATGCTGAAGAACTTATTTAACCTGTTTTTGAAGCTCAAAAAGAATAATAATAATAACATGGCTTCTTTCACGTTCAAAGCGGCGGCGCAGGTGGTGCTTATGGTTCTTGCTGTTACTCTTCAGCCGCAGGCGGCGGAGGGCCTCACCGTCGGCAAGGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGGCTCTTCCTGCAGAAACGTAAGCATGTGCTGCCCCACCAATGGTTGCTGCCACCGAGCTGAATTTCCCGCAGAGTTTACAATCCGGTGGCCTTCATGTTGTACTAATGAAACCTTCAACGAAAATGAGGTTAGTTCCTTGTTTGAAGATCTTGACCGTTACTGGCCAAGCTATCGGTGTGGTCTCGTGAGCAGCTGCGATAACAAAAAAGGATCATTTTGGGCTCACCAGGTTCAAATTCTCGATATGGTATAGAAGCATGGAACCTGTGGATCACCTGTAATCCTCCACCAATATGATTACTTCTTCACAACTCTCGCCCTTTTCTTTAACTACAATGTCACTGATGCTGAAATTGTAGCTTCTGATACCAAAAAGTATGGTATTGAAGATGTGTTTGAAGCTGTTCGGTCTGCATTTAAAGCGAATCCAAAGCTTAGCTGCGGTAAAAACGGGATTATTAAGGAACTTCGTCTATGCTTTGATAAAGAATTCAAGGTAATCGATTGTGATGCTACAAAATCATGTCCCAAATTTGTTCGTCTACCAGAATTCCATGAACTACCAAGGTCGACCATGGTTAAGAGAGCAATTCCAAGAATGATTGACGTTGAAGCAACTTCATTTTTTTCGTCAAACAAAGTGTGAAATTCAAGAGTATTTGAATTAATTTAGTGAAATAGTTAACTTAGTTTGTCGAATAACAATTCACAAAAGTTTCCATCTTTGTC

Coding sequence (CDS)

ATGGCTTCTTTCTCCATTAAATCCAACCCCATGGTGGTGGCGCCGGCGGTGTTTCTTCTTGTTCTTCAACTGGTGGCGGTGGTACTCGCGGCGGAGACTCCAAGCGGGGGCGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGCTACTTCTTGCAAGAACACTGGAAAGTGTTGCCCGGCCAATGGTTGCTGCCGTGGAGCTGACTCTCCCACTGAATTTACAATCCATGGGCTATGGCCTCAGTATAATGAGAAAGGATGGCCATCTTGTTGCACTGATGCAGTCTTCAGTCAAAATGAGGATCATTTTGGGCTCATGAGGTTCATATCACTCTCTCTTTGTTCTTCCTTAGCTCATCTGTCAAACTTTGAGACTAATCCAAAATTCCTTTCCTTTTTCTCTTCTTCTCATATGATCGCCATTATCATATCAAATGCTACCATTGCTGCAGTATGGTCAAAAGCGCTGAGCGATGCTGGGTTCGTAGCTTCCAATACTGAGAAGTATCCACTAGAAGATGTGGTGGATGCAGTCAAGAATGCTACCAATTCAACTCCAAAGATTAGCTGTGCTAAAAAGGGGGCTGTCAAGGAGCTTTGGATATGCTTTGACAAGAAATTTGAGGTGTTCTTTGTGTTCATAACAATCATATCTAATAAGCTTTTGGGTTGGATCAAATTGTTCATAGTTTTTGGTTGTTGCAGCCACGTGACTGTGATCTTGCCAATTCATGCCCCGAGTTTATTAAGCTCCCAACGTATGAGCCTCAGGATGTGGTGGAGGGAGCTATTCCATGGATTGCTGATGCTGAAGAACTTATTTAACCTGTTTTTGAAGCTCAAAAAGAATAATAATAATAACATGGCTTCTTTCACGTTCAAAGCGGCGGCGCAGGTGGTGCTTATGGTTCTTGCTGTTACTCTTCAGCCGCAGGCGGCGGAGGGCCTCACCGTCGGCAAGGAGCAGAGGGAATTTGACTACTTTGTGTTGGCTCTTCAATGGCCAGGCTCTTCCTGCAGAAACGTAAGCATGTGCTGCCCCACCAATGGTTGCTGCCACCGAGCTGAATTTCCCGCAGAGTTTACAATCCGGTGGCCTTCATGTTGTACTAATGAAACCTTCAACGAAAATGAGGTTAGTTCCTTGTTTGAAGATCTTGACCGTTACTGGCCAAGCTATCGGTGTGGTCTCGTGAGCAGCTGCGATAACAAAAAAGGATCATTTTGGGCTCACCAGGTTCAAATTCTCGATATGGTATAG

Protein sequence

MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEDHFGLMRFISLSLCSSLAHLSNFETNPKFLSFFSSSHMIAIIISNATIAAVWSKALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEVFFVFITIISNKLLGWIKLFIVFGCCSHVTVILPIHAPSLLSSQRMSLRMWWRELFHGLLMLKNLFNLFLKLKKNNNNNMASFTFKAAAQVVLMVLAVTLQPQAAEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCPTNGCCHRAEFPAEFTIRWPSCCTNETFNENEVSSLFEDLDRYWPSYRCGLVSSCDNKKGSFWAHQVQILDMV
Homology
BLAST of CmoCh16G002670 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.9e-33
Identity = 81/209 (38.76%), Postives = 115/209 (55.02%), Query Frame = 0

Query: 19  LLVLQLVAVVLAAET-PSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTE 78
           LL++  +A   A +       QREFDYF L+LQWP T C+ T  CC  N CCRG+D+PT+
Sbjct: 8   LLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQ 67

Query: 79  FTIHGLWPQYNEKGWPSCCTDAVFSQNEDHF---GLMRFISLSLCSSLAHLS-------- 138
           FTIHGLWP YN+  WPSCC  + F + E      GL ++     C S +  +        
Sbjct: 68  FTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWG 127

Query: 139 -NFETNPKFLS-FFSSSHMIAIIISNATIAAVWSKALSDAGFVASNTEKYPLEDVVDAVK 198
             +E +    S  F   +   +   N  +    +  L  AG+VASN+EKYPL  +V A++
Sbjct: 128 HEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQ 187

Query: 199 NATNSTPKISCAKKGAVKELWICFDKKFE 214
           NA + TP++ C K+ A+ E+ ICF K F+
Sbjct: 188 NAFHITPEVVC-KRDAIDEIRICFYKDFK 215

BLAST of CmoCh16G002670 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 1.1e-08
Identity = 51/205 (24.88%), Postives = 81/205 (39.51%), Query Frame = 0

Query: 40  REFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTD 99
           ++FD+F    QWPA+ C     CC P  G         +F+IHGLWP Y +  WP  C D
Sbjct: 26  QDFDFFYFVQQWPASYCDTRRSCCYPTTG-----KPDEDFSIHGLWPNYKDGKWPQNC-D 85

Query: 100 AVFSQNEDHF--------------------GLM----RFISLSLCSSLAHLSNFETNPKF 159
              S +E  F                    GL      ++    CS+L   + F+T    
Sbjct: 86  RESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTCSALNQHAYFQT---A 145

Query: 160 LSFFSSSHMIAIIISNATIAAVWSKALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKIS 216
           L F + S+++              + L++AG    N + Y +E +  A++     TP I 
Sbjct: 146 LDFKTKSNLL--------------QNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIE 205

BLAST of CmoCh16G002670 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.2e-08
Identity = 52/203 (25.62%), Postives = 86/203 (42.36%), Query Frame = 0

Query: 20  LVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTEFT 79
           L L  + V+L +   +     +FD+F    QWP + C    KCC  N     AD    F 
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD----FG 67

Query: 80  IHGLWPQYNEKGWPSCCTDAVFSQNEDHFGLMRFISLS----LCSSLAHLSNFETNPKFL 139
           IHGLWP Y +  +PS C  +    +     L+  +  S     C S +  + +E   +  
Sbjct: 68  IHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKH 127

Query: 140 SFFSSS----HMIAIIISNATIAAVWSKALSDAGFVASNTEKYPLEDVVDAVKNATNSTP 199
              S S    H       N         AL+ AG +  + + Y LE + D++K +   TP
Sbjct: 128 GTCSESVIDQHEYFQTALNLKQKTNLLGALTKAG-INPDGKSYSLESIRDSIKESIGFTP 187

Query: 200 KISCAKKGA----VKELWICFDK 211
            + C + G+    + ++++C D+
Sbjct: 188 WVECNRDGSGNSQLYQVYLCVDR 205

BLAST of CmoCh16G002670 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.5e-07
Identity = 30/81 (37.04%), Postives = 38/81 (46.91%), Query Frame = 0

Query: 17 VFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSP 76
          +F+L LQ + V   A+        +FD+F   LQWP   C +   CC P  G        
Sbjct: 5  IFILALQQLYVQSFAQ--------DFDFFYFVLQWPGAYCDSRHSCCYPQTG-----KPA 64

Query: 77 TEFTIHGLWPQYNEKGWPSCC 97
           +F IHGLWP Y   GWP  C
Sbjct: 65 ADFGIHGLWPNYKTGGWPQNC 72

BLAST of CmoCh16G002670 vs. ExPASy Swiss-Prot
Match: Q38716 (Ribonuclease S-2 OS=Antirrhinum hispanicum OX=49039 GN=S2 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 6.7e-06
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 0

Query: 41 EFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWP-SCCTD 99
          +FDYF L LQWP + C      CP          P++FTIHGLWP  + K WP S C D
Sbjct: 32 QFDYFKLVLQWPNSYCSLKTTHCPRTRL------PSQFTIHGLWP--DNKSWPLSNCRD 82

BLAST of CmoCh16G002670 vs. ExPASy TrEMBL
Match: A0A6J1EA39 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 1.6e-82
Identity = 171/232 (73.71%), Postives = 179/232 (77.16%), Query Frame = 0

Query: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60
           MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60

Query: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQN---------EDHFGLM 120
           KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQN         E ++   
Sbjct: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPTY 120

Query: 121 RFISLSLC-----SSLAHLSNFETN-----PKFLSFFSSSHMIAIIISNATIAAVWSKAL 180
           R  + S C     S  AH   +E +     P  +  F        I S   +    +KAL
Sbjct: 121 RCGTTSNCHGTKGSFWAH--EYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNV----TKAL 180

Query: 181 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 214
           SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE
Sbjct: 181 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 226

BLAST of CmoCh16G002670 vs. ExPASy TrEMBL
Match: A0A6J1J670 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 1.1e-75
Identity = 159/230 (69.13%), Postives = 174/230 (75.65%), Query Frame = 0

Query: 1   MASFSIKSNPMVVAPAVFLLV-LQLVAVVLAAETPS-GGEQREFDYFVLALQWPATSCKN 60
           MASF IKSNPMVVAPAVFLL+ LQLVAVVLAAETPS GG Q+EFDYFVLALQWPATSCKN
Sbjct: 1   MASFYIKSNPMVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKN 60

Query: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEDHF---GLMRFIS 120
           TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVF+QNE       + ++  
Sbjct: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWP 120

Query: 121 LSLCSSLAHLSNFETNPKFLSFFSSSHMIA----------IIISNATIAAVW--SKALSD 180
              C + ++     T   F +     H               ++  TI + +  +KALSD
Sbjct: 121 TYRCGTTSNCHG--TKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKALSD 180

Query: 181 AGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 214
           AGFVASNTEKYPL DVVDAVKNATNSTPKISCAKKGAVKELWICFDK FE
Sbjct: 181 AGFVASNTEKYPLGDVVDAVKNATNSTPKISCAKKGAVKELWICFDKNFE 228

BLAST of CmoCh16G002670 vs. ExPASy TrEMBL
Match: A0A6J1E4S1 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430736 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 9.2e-67
Identity = 124/137 (90.51%), Postives = 125/137 (91.24%), Query Frame = 0

Query: 293 MASFTFKAAAQVVLMVLAVTLQPQAAEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCP 352
           MASFTFKAAAQVVLMVLAVTLQPQAAEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCP
Sbjct: 1   MASFTFKAAAQVVLMVLAVTLQPQAAEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCP 60

Query: 353 TNGCCHRAEFPAEFTIR----------WPSCCTNETFNENEVSSLFEDLDRYWPSYRCGL 412
           TNGCCHRAEFPAEFTI           WPSCCTNETFNENEVSSLFEDLDRYWPSYRCGL
Sbjct: 61  TNGCCHRAEFPAEFTIHGLWPQYQEKGWPSCCTNETFNENEVSSLFEDLDRYWPSYRCGL 120

Query: 413 VSSCDNKKGSFWAHQVQ 420
           VSSCDNKKGSFWAHQ +
Sbjct: 121 VSSCDNKKGSFWAHQYE 137

BLAST of CmoCh16G002670 vs. ExPASy TrEMBL
Match: A0A6J1JAL4 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111483248 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 6.1e-63
Identity = 117/137 (85.40%), Postives = 120/137 (87.59%), Query Frame = 0

Query: 293 MASFTFKAAAQVVLMVLAVTLQPQAAEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCP 352
           MASFTFKAAAQVV MVLAVTL PQA EGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCP
Sbjct: 1   MASFTFKAAAQVVFMVLAVTLPPQAVEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCP 60

Query: 353 TNGCCHRAEFPAEFTIR----------WPSCCTNETFNENEVSSLFEDLDRYWPSYRCGL 412
           TNGCCHRAE PAEFTI           WPSCCTNETFNENEVSSLFEDL+RYWPSYRCGL
Sbjct: 61  TNGCCHRAELPAEFTIHGLWPQYQDKGWPSCCTNETFNENEVSSLFEDLERYWPSYRCGL 120

Query: 413 VSSCDNKKGSFWAHQVQ 420
           VSSC N+KGSFWAHQ +
Sbjct: 121 VSSCSNRKGSFWAHQYE 137

BLAST of CmoCh16G002670 vs. ExPASy TrEMBL
Match: A0A5D3E236 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold652G00240 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.2e-61
Identity = 127/225 (56.44%), Postives = 153/225 (68.00%), Query Frame = 0

Query: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVV--LAAETPSGGEQREFDYFVLALQWPATSCKN 60
           MASFSIK  PMV       L+++L+ VV   +AE PSG EQREFDYFVLALQWPATSCKN
Sbjct: 1   MASFSIKGKPMV----FLFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKN 60

Query: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNE---------DHFG 120
            GKCCP+N CCRGADSPTEFTIHGLWPQYNEKGWPSCCTDA F++NE          ++ 
Sbjct: 61  AGKCCPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWP 120

Query: 121 LMRFISLSLCSSLAHLSNFETNPKFLSFFSSSHMIAIIISNATIAAVWSKALSDAGFVAS 180
             R      C++   +  ++     L+ FS  ++              +K LS+ GFVAS
Sbjct: 121 TYRCEKHGTCAAPVLVGEYDYFLTALTIFSKYNV--------------TKVLSEGGFVAS 180

Query: 181 NTEKYPLEDVVDAVKNA-TNSTPKISCAKKGAVKELWICFDKKFE 214
           NTEKYP+EDV+ A+KN   N+TPKI+CAKKG VKELW+CFDK FE
Sbjct: 181 NTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFE 207

BLAST of CmoCh16G002670 vs. NCBI nr
Match: KAG7014875.1 (Ribonuclease 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 391.3 bits (1004), Expect = 1.0e-104
Identity = 219/361 (60.66%), Postives = 233/361 (64.54%), Query Frame = 0

Query: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60
           MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60

Query: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEDHFGLMRFISLSLCS 120
           KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDA+    ED   + ++     C 
Sbjct: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAISLLRED---IEKYWPTYGCG 120

Query: 121 SLAHLSNFETNPKFLSFFSSSHMIAIIISNATIAAVWS--KALSDAGFVASNTEKYPLED 180
           + ++                         + T  + W+  KALSDAGFVASNTEKYPLED
Sbjct: 121 TTSN------------------------CHGTKGSFWAHEKALSDAGFVASNTEKYPLED 180

Query: 181 VVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEVFFVFITIISNKLLGWIKLFIVFGC 240
           VVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE                         
Sbjct: 181 VVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE------------------------- 240

Query: 241 CSHVTVILPIHAPSLLSSQRMSLRMWWRELFHGLLMLKNLFNLFLKLKKNNNNNMASFTF 300
                   P                           L N    F+KL           T+
Sbjct: 241 --------PRDCD-----------------------LANSCPEFIKLP----------TY 268

Query: 301 KAAAQVVLMVLAVTLQPQAAEGLTVGKEQREFDYFVLALQWPGSSCRNVSMCCPTNGCCH 360
           +    VV MVLAVTL PQAAEGLTVG+EQREFDYFVLALQWPGSSCRNVSMCCPTNGCCH
Sbjct: 301 EPQDVVVFMVLAVTLPPQAAEGLTVGREQREFDYFVLALQWPGSSCRNVSMCCPTNGCCH 268

BLAST of CmoCh16G002670 vs. NCBI nr
Match: XP_022922895.1 (ribonuclease 2-like [Cucurbita moschata])

HSP 1 Score: 316.6 bits (810), Expect = 3.2e-82
Identity = 171/232 (73.71%), Postives = 179/232 (77.16%), Query Frame = 0

Query: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60
           MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60

Query: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQN---------EDHFGLM 120
           KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQN         E ++   
Sbjct: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPTY 120

Query: 121 RFISLSLC-----SSLAHLSNFETN-----PKFLSFFSSSHMIAIIISNATIAAVWSKAL 180
           R  + S C     S  AH   +E +     P  +  F        I S   +    +KAL
Sbjct: 121 RCGTTSNCHGTKGSFWAH--EYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNV----TKAL 180

Query: 181 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 214
           SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE
Sbjct: 181 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 226

BLAST of CmoCh16G002670 vs. NCBI nr
Match: KAG6576854.1 (Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 302.4 bits (773), Expect = 6.3e-78
Identity = 159/232 (68.53%), Postives = 172/232 (74.14%), Query Frame = 0

Query: 11  MVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 70
           MVVAPAVFLLVLQLVAVV+AAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR
Sbjct: 1   MVVAPAVFLLVLQLVAVVIAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCR 60

Query: 71  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQN---------EDHFGLMRFISLSLCS- 130
           GADSPTEFTIHGLWPQYNEKGWPSCCTDAVF+QN         E ++   R  + S C  
Sbjct: 61  GADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLREDIEKYWPTYRCGTTSNCHV 120

Query: 131 -------------SLAHLSNFETNPKFLSFFSSSHMIAII------ISNATIAAVWSKAL 190
                         +   SN   NP F     +     I+      ++  TI + ++KAL
Sbjct: 121 WSVSSSLPIFLELDILFYSNSNPNPPFSEKHGTCAAPVIVGEYDYFLTTLTIFSKYNKAL 180

Query: 191 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 214
           SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE
Sbjct: 181 SDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 232

BLAST of CmoCh16G002670 vs. NCBI nr
Match: XP_023553229.1 (ribonuclease 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.0 bits (772), Expect = 8.2e-78
Identity = 159/228 (69.74%), Postives = 173/228 (75.88%), Query Frame = 0

Query: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTG 60
           MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGG QREFDYFVLALQWPATSCKNTG
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAETPSGGGQREFDYFVLALQWPATSCKNTG 60

Query: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEDHF---GLMRFISLS 120
           KCCPANGCCRGADSPTEFTIHGLWPQYNE+GWPSCCTDAVF+QNE       + ++    
Sbjct: 61  KCCPANGCCRGADSPTEFTIHGLWPQYNERGWPSCCTDAVFNQNEISLLEEDIQKYWPTY 120

Query: 121 LCSSLAHLSNFETNPKFLSFFSSSHMIA----------IIISNATIAAVW--SKALSDAG 180
            C +  +     T   F +     H               ++  TI + +  +KALSDAG
Sbjct: 121 RCGTTGNCHG--TKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKALSDAG 180

Query: 181 FVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 214
           FVASNTEKY L DVVDAVKNAT+STPKISCAKKGAVKELWICFDK FE
Sbjct: 181 FVASNTEKYALGDVVDAVKNATDSTPKISCAKKGAVKELWICFDKNFE 226

BLAST of CmoCh16G002670 vs. NCBI nr
Match: XP_022984766.1 (ribonuclease 2-like [Cucurbita maxima])

HSP 1 Score: 293.9 bits (751), Expect = 2.2e-75
Identity = 159/230 (69.13%), Postives = 174/230 (75.65%), Query Frame = 0

Query: 1   MASFSIKSNPMVVAPAVFLLV-LQLVAVVLAAETPS-GGEQREFDYFVLALQWPATSCKN 60
           MASF IKSNPMVVAPAVFLL+ LQLVAVVLAAETPS GG Q+EFDYFVLALQWPATSCKN
Sbjct: 1   MASFYIKSNPMVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKN 60

Query: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEDHF---GLMRFIS 120
           TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVF+QNE       + ++  
Sbjct: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWP 120

Query: 121 LSLCSSLAHLSNFETNPKFLSFFSSSHMIA----------IIISNATIAAVW--SKALSD 180
              C + ++     T   F +     H               ++  TI + +  +KALSD
Sbjct: 121 TYRCGTTSNCHG--TKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKALSD 180

Query: 181 AGFVASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFE 214
           AGFVASNTEKYPL DVVDAVKNATNSTPKISCAKKGAVKELWICFDK FE
Sbjct: 181 AGFVASNTEKYPLGDVVDAVKNATNSTPKISCAKKGAVKELWICFDKNFE 228

BLAST of CmoCh16G002670 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 143.7 bits (361), Expect = 3.5e-34
Identity = 81/209 (38.76%), Postives = 115/209 (55.02%), Query Frame = 0

Query: 19  LLVLQLVAVVLAAET-PSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTE 78
           LL++  +A   A +       QREFDYF L+LQWP T C+ T  CC  N CCRG+D+PT+
Sbjct: 8   LLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQ 67

Query: 79  FTIHGLWPQYNEKGWPSCCTDAVFSQNEDHF---GLMRFISLSLCSSLAHLS-------- 138
           FTIHGLWP YN+  WPSCC  + F + E      GL ++     C S +  +        
Sbjct: 68  FTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWG 127

Query: 139 -NFETNPKFLS-FFSSSHMIAIIISNATIAAVWSKALSDAGFVASNTEKYPLEDVVDAVK 198
             +E +    S  F   +   +   N  +    +  L  AG+VASN+EKYPL  +V A++
Sbjct: 128 HEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQ 187

Query: 199 NATNSTPKISCAKKGAVKELWICFDKKFE 214
           NA + TP++ C K+ A+ E+ ICF K F+
Sbjct: 188 NAFHITPEVVC-KRDAIDEIRICFYKDFK 215

BLAST of CmoCh16G002670 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 143.7 bits (361), Expect = 3.5e-34
Identity = 82/198 (41.41%), Postives = 109/198 (55.05%), Query Frame = 0

Query: 19  LLVLQLVAVVLAAET-PSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTE 78
           LL++  +A   A +       QREFDYF L+LQWP T C+ T  CC  N CCRG+D+PT+
Sbjct: 8   LLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQ 67

Query: 79  FTIHGLWPQYNEKGWPSCCTDAVFSQNEDHFGLMRFISLSLCSSLAHLSNFETNPKFLSF 138
           FTIHGLWP YN+  WPSCC  + F + E              S+L  +   E     LS 
Sbjct: 68  FTIHGLWPDYNDGSWPSCCYRSDFKEKE-------------ISTL--MDGLEKYWPSLSC 127

Query: 139 FSSSHMIAIIISNATIAAVW--SKALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISC 198
            S S        N    + W     L  AG+VASN+EKYPL  +V A++NA + TP++ C
Sbjct: 128 GSPSS------CNGGKGSFWGHEDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVC 183

Query: 199 AKKGAVKELWICFDKKFE 214
            K+ A+ E+ ICF K F+
Sbjct: 188 -KRDAIDEIRICFYKDFK 183

BLAST of CmoCh16G002670 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 60.8 bits (146), Expect = 3.0e-09
Identity = 52/203 (25.62%), Postives = 86/203 (42.36%), Query Frame = 0

Query: 20  LVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCCPANGCCRGADSPTEFT 79
           L L  + V+L +   +     +FD+F    QWP + C    KCC  N     AD    F 
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAAD----FG 67

Query: 80  IHGLWPQYNEKGWPSCCTDAVFSQNEDHFGLMRFISLS----LCSSLAHLSNFETNPKFL 139
           IHGLWP Y +  +PS C  +    +     L+  +  S     C S +  + +E   +  
Sbjct: 68  IHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKH 127

Query: 140 SFFSSS----HMIAIIISNATIAAVWSKALSDAGFVASNTEKYPLEDVVDAVKNATNSTP 199
              S S    H       N         AL+ AG +  + + Y LE + D++K +   TP
Sbjct: 128 GTCSESVIDQHEYFQTALNLKQKTNLLGALTKAG-INPDGKSYSLESIRDSIKESIGFTP 187

Query: 200 KISCAKKGA----VKELWICFDK 211
            + C + G+    + ++++C D+
Sbjct: 188 WVECNRDGSGNSQLYQVYLCVDR 205

BLAST of CmoCh16G002670 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 60.1 bits (144), Expect = 5.0e-09
Identity = 51/184 (27.72%), Postives = 75/184 (40.76%), Query Frame = 0

Query: 39  QREFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSPTEFTIHGLWPQYNEKGWPS-CC 98
           Q +FD+F   LQWP   C  +  CC P +G         +F IHGLWP YN   WPS C 
Sbjct: 25  QEDFDFFYFVLQWPGAYCDTSRACCYPTSG-----KPAADFGIHGLWPNYNGGSWPSNCD 84

Query: 99  TDAVF--SQNEDHFGLMRFISLSLCSSLAHLSNF-----ETNPKFLSFFSSSHMIAIIIS 158
            D+ F  SQ  D    ++    +L        NF     E +          H       
Sbjct: 85  PDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENAL 144

Query: 159 NATIAAVWSKALSDAGFVASNTEKYPLEDVVDAVKNATNSTPKISCAK----KGAVKELW 210
                A   + L ++G +  +   Y L+ + +A+K+    TP I C K       + +++
Sbjct: 145 KLKQKANLLQILKNSG-INPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNAQLHQIY 202

BLAST of CmoCh16G002670 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 57.8 bits (138), Expect = 2.5e-08
Identity = 30/81 (37.04%), Postives = 38/81 (46.91%), Query Frame = 0

Query: 17 VFLLVLQLVAVVLAAETPSGGEQREFDYFVLALQWPATSCKNTGKCC-PANGCCRGADSP 76
          +F+L LQ + V   A+        +FD+F   LQWP   C +   CC P  G        
Sbjct: 5  IFILALQQLYVQSFAQ--------DFDFFYFVLQWPGAYCDSRHSCCYPQTG-----KPA 64

Query: 77 TEFTIHGLWPQYNEKGWPSCC 97
           +F IHGLWP Y   GWP  C
Sbjct: 65 ADFGIHGLWPNYKTGGWPQNC 72

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P428144.9e-3338.76Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1[more]
P801961.1e-0824.88Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2[more]
P428134.2e-0825.62Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
P428153.5e-0737.04Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
Q387166.7e-0645.76Ribonuclease S-2 OS=Antirrhinum hispanicum OX=49039 GN=S2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EA391.6e-8273.71Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1[more]
A0A6J1J6701.1e-7569.13Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1[more]
A0A6J1E4S19.2e-6790.51Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430736 PE=3 SV=1[more]
A0A6J1JAL46.1e-6385.40Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111483248 PE=3 SV=1[more]
A0A5D3E2361.2e-6156.44Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold652G00... [more]
Match NameE-valueIdentityDescription
KAG7014875.11.0e-10460.66Ribonuclease 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022922895.13.2e-8273.71ribonuclease 2-like [Cucurbita moschata][more]
KAG6576854.16.3e-7868.53Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023553229.18.2e-7869.74ribonuclease 2-like [Cucurbita pepo subsp. pepo][more]
XP_022984766.12.2e-7569.13ribonuclease 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G39780.13.5e-3438.76ribonuclease 2 [more]
AT2G39780.23.5e-3441.41ribonuclease 2 [more]
AT2G02990.13.0e-0925.62ribonuclease 1 [more]
AT1G14220.15.0e-0927.72Ribonuclease T2 family protein [more]
AT1G26820.12.5e-0837.04ribonuclease 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 38..134
e-value: 9.8E-21
score: 76.7
coord: 326..419
e-value: 2.0E-19
score: 72.4
coord: 151..220
e-value: 4.4E-12
score: 48.4
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 41..214
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 329..417
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 158..214
e-value: 8.1E-9
score: 35.9
coord: 330..417
e-value: 2.6E-6
score: 27.7
coord: 42..104
e-value: 5.9E-11
score: 42.8
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 37..213
coord: 304..419
NoneNo IPR availablePANTHERPTHR11240:SF53RIBONUCLEASE 2-LIKE ISOFORM X1coord: 37..213
NoneNo IPR availablePANTHERPTHR11240:SF53RIBONUCLEASE 2-LIKE ISOFORM X1coord: 304..419
NoneNo IPR availableCDDcd00374RNase_T2coord: 42..215
e-value: 1.71191E-24
score: 97.9119
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 78..85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G002670.1CmoCh16G002670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding