CmoCh16G001730 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G001730
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	subtilisin-like protease SBT1.3
Location	Cmo_Chr16: 786400 .. 788718 (-)
RNA-Seq Expression	CmoCh16G001730
Synteny	CmoCh16G001730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCACACTCCTCTGAAATGGCTGTGTTTGCTGCTGGTTTTTGACTGTTTGCTTCTTTCAAGCGCTCAGTTTATGAAAACTTATGTTGTTCAAATGGATAGGTCTGCAATGCCGGACTCATTTTCGGATCATCTTCAGTGGTACTCCACAGTGCTTACCAGTGTGGTCGTTAATCCTGAGAGAGAAGGCAATGGGGGAGGTGAACAGAGGATCATTTACAGTTACCAGAATGTTTTCCATGGAGTTGCAGCTCGATTGAATGAAGAAGAGGCAGAGCGGCTCGAGGAAGAAAATGGGGTCTTGGCCATTTTTCCAGAGACCAAGTACGAGCTTCACACCACCAGAAGCCCCAGATTTCTTGGGCTTGAACCAGCTGACAGCAATAGCGCCTGGTCTCAGCAAATTGCAGACCATGACGTGATTGTTGGAGTTTTGGACACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGATGCTGGGATGTCGCCGGTGCCGGCGCATTGGAAAGGGGAATGCGAAACAGGGCGAGACTTCACGAAACAGAATTGTAACAGAAAGATCGTTGGAGCTAGAATGTTCTACCATGGGTATGAAGCTGCAACTGGGAAATTTAACGAACAGTTGGAGTATAAATCTCCAAGGGATCAAGATGGGCATGGAACTCACACGGCAGCCACCGTCGCCGGCTCTCCGACAGCCGGCGCGAACCTCCTCGGTTATGCGTATGGGACGGCCAGAGGAATGGCGCCCGGTGCTAGAATAGCGGCTTACAAAGTCTGTTGGGCCGGCGGCTGTTTTAGCTCCGACATTTTGTCGGCTGTCGACAGAGCTGTGGCCGATGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTCTCCGTTGCAGCATTTGGTGCAATGGAGATGGGCGTGTTTGTGTCCTGTTCGGCCGGAAATGCAGGGCCGGACCCTGTCAGTCTCACAAACGTATCGCCATGGATAACCACTGTCGGTGCCAGCACAATGGACAGAGATTTCCCGGCCATTGTCAAGCTCGGCAATGGAAGAACAGTCACCGGCGTTTCACTTTACAAAGGAAGAATCACAATCCCAGAAAGCAAACAATTCCCAGTTGTATACATGGGGAGTAACTCAAGCAACCCGGATCCGAGCTCGCTGTGTTTAGAAGGAACATTAGATCCCCATTTCGTGGCCGGAAAAATCGTGATATGCGATCGTGGAATTAGCCCACGGGTCCAGAAGGGTGTGGTGGTGAAGAATGCAGGTGGGGTTGGGATGATTCTGGCGAACACGGCGGCGAATGGGGAAGAACTTGTTGCCGATTGCCATTTGGTACCTGCCGTCGCCGTCGGTGAAAGAGAAGGTAAAGCAATTAAGCTGTACGCATTGACGAATCGAAAACCGACGGCGACTTTAGGATTTTTAGGGACAAGATTGGGGGTAAGACCCTCGCCGGTGGTGGCGGCATTTTCATCCAGAGGACCAAATTTCCTCACACTTGAGATCCTCAAGCCCGATCTGGTGGCCCCTGGCGTTAACATCCTTGCCGCTTGGACGGGAAAAACAGGGCCATCGAGTTTGACGACGGATACAAGAAGAGTTAAATTCAACATTCTGTCAGGAACCTCAATGTCTTGCCCACACGTCAGTGGCGTAGCAGCTCTGATCAAATCAAAGCATCCCGATTGGAGCCCGTCGGCAATCAAATCGGCACTGATGACCACCGCTTACGTCCACGACAACACCTATAAGCCTCTCAAAGACTCCTCCGGTGCATCGCCGTCGAGTCCGTACGACCACGGCGCCGGCCACATAAACCCAAGAAAAGCCCTAGATCCTGGCTTGGTTTACGAAATTCAGCCGCAAGATTACTTCGAATTCCTCTGTACGCAGGATTTAACCCCTTCACAGCTCAAAGTATTCTCCAAATACTCAAACAGATCTTGTCATCACCTCCTCCCCAACCCTGGAGACTTAAATTATCCGGCCATCTCTGCCGTTTTCCCCGAAAAAACCTCCGTCACTTCCTTGACCCTTCACAGAACCGTCACCAACGTCGGCCCTGCAACCTCCAGTTACCATGCCGTGGTGACTCCGTTCAACGGCGCCGCCGTGAAAGTTGAGCCGGAGAGTCTGAACTTCACTAGGAGATATCAGAAGCTTTCATACAAAATCACTTTCCTGACGAAGAAGAGACAGAGTATGCCGGAATTCGGAGGATTAATCTGGAAGGACGGAACTCATAGTGTGAGAAGTCCGATTGTGATCACTTGGTTGTCGTTTGTTTGA

mRNA sequence

ATGGCTCACACTCCTCTGAAATGGCTGTGTTTGCTGCTGGTTTTTGACTGTTTGCTTCTTTCAAGCGCTCAGTTTATGAAAACTTATGTTGTTCAAATGGATAGGTCTGCAATGCCGGACTCATTTTCGGATCATCTTCAGTGGTACTCCACAGTGCTTACCAGTGTGGTCGTTAATCCTGAGAGAGAAGGCAATGGGGGAGGTGAACAGAGGATCATTTACAGTTACCAGAATGTTTTCCATGGAGTTGCAGCTCGATTGAATGAAGAAGAGGCAGAGCGGCTCGAGGAAGAAAATGGGGTCTTGGCCATTTTTCCAGAGACCAAGTACGAGCTTCACACCACCAGAAGCCCCAGATTTCTTGGGCTTGAACCAGCTGACAGCAATAGCGCCTGGTCTCAGCAAATTGCAGACCATGACGTGATTGTTGGAGTTTTGGACACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGATGCTGGGATGTCGCCGGTGCCGGCGCATTGGAAAGGGGAATGCGAAACAGGGCGAGACTTCACGAAACAGAATTGTAACAGAAAGATCGTTGGAGCTAGAATGTTCTACCATGGGTATGAAGCTGCAACTGGGAAATTTAACGAACAGTTGGAGTATAAATCTCCAAGGGATCAAGATGGGCATGGAACTCACACGGCAGCCACCGTCGCCGGCTCTCCGACAGCCGGCGCGAACCTCCTCGGTTATGCGTATGGGACGGCCAGAGGAATGGCGCCCGGTGCTAGAATAGCGGCTTACAAAGTCTGTTGGGCCGGCGGCTGTTTTAGCTCCGACATTTTGTCGGCTGTCGACAGAGCTGTGGCCGATGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTCTCCGTTGCAGCATTTGGTGCAATGGAGATGGGCGTGTTTGTGTCCTGTTCGGCCGGAAATGCAGGGCCGGACCCTGTCAGTCTCACAAACGTATCGCCATGGATAACCACTGTCGGTGCCAGCACAATGGACAGAGATTTCCCGGCCATTGTCAAGCTCGGCAATGGAAGAACAGTCACCGGCGTTTCACTTTACAAAGGAAGAATCACAATCCCAGAAAGCAAACAATTCCCAGTTGTATACATGGGGAGTAACTCAAGCAACCCGGATCCGAGCTCGCTGTGTTTAGAAGGAACATTAGATCCCCATTTCGTGGCCGGAAAAATCGTGATATGCGATCGTGGAATTAGCCCACGGGTCCAGAAGGGTGTGGTGGTGAAGAATGCAGGTGGGGTTGGGATGATTCTGGCGAACACGGCGGCGAATGGGGAAGAACTTGTTGCCGATTGCCATTTGGTACCTGCCGTCGCCGTCGGTGAAAGAGAAGGTAAAGCAATTAAGCTGTACGCATTGACGAATCGAAAACCGACGGCGACTTTAGGATTTTTAGGGACAAGATTGGGGGTAAGACCCTCGCCGGTGGTGGCGGCATTTTCATCCAGAGGACCAAATTTCCTCACACTTGAGATCCTCAAGCCCGATCTGGTGGCCCCTGGCGTTAACATCCTTGCCGCTTGGACGGGAAAAACAGGGCCATCGAGTTTGACGACGGATACAAGAAGAGTTAAATTCAACATTCTGTCAGGAACCTCAATGTCTTGCCCACACGTCAGTGGCGTAGCAGCTCTGATCAAATCAAAGCATCCCGATTGGAGCCCGTCGGCAATCAAATCGGCACTGATGACCACCGCTTACGTCCACGACAACACCTATAAGCCTCTCAAAGACTCCTCCGGTGCATCGCCGTCGAGTCCGTACGACCACGGCGCCGGCCACATAAACCCAAGAAAAGCCCTAGATCCTGGCTTGGTTTACGAAATTCAGCCGCAAGATTACTTCGAATTCCTCTGTACGCAGGATTTAACCCCTTCACAGCTCAAAGTATTCTCCAAATACTCAAACAGATCTTGTCATCACCTCCTCCCCAACCCTGGAGACTTAAATTATCCGGCCATCTCTGCCGTTTTCCCCGAAAAAACCTCCGTCACTTCCTTGACCCTTCACAGAACCGTCACCAACGTCGGCCCTGCAACCTCCAGTTACCATGCCGTGGTGACTCCGTTCAACGGCGCCGCCGTGAAAGTTGAGCCGGAGAGTCTGAACTTCACTAGGAGATATCAGAAGCTTTCATACAAAATCACTTTCCTGACGAAGAAGAGACAGAGTATGCCGGAATTCGGAGGATTAATCTGGAAGGACGGAACTCATAGTGTGAGAAGTCCGATTGTGATCACTTGGTTGTCGTTTGTTTGA

Coding sequence (CDS)

ATGGCTCACACTCCTCTGAAATGGCTGTGTTTGCTGCTGGTTTTTGACTGTTTGCTTCTTTCAAGCGCTCAGTTTATGAAAACTTATGTTGTTCAAATGGATAGGTCTGCAATGCCGGACTCATTTTCGGATCATCTTCAGTGGTACTCCACAGTGCTTACCAGTGTGGTCGTTAATCCTGAGAGAGAAGGCAATGGGGGAGGTGAACAGAGGATCATTTACAGTTACCAGAATGTTTTCCATGGAGTTGCAGCTCGATTGAATGAAGAAGAGGCAGAGCGGCTCGAGGAAGAAAATGGGGTCTTGGCCATTTTTCCAGAGACCAAGTACGAGCTTCACACCACCAGAAGCCCCAGATTTCTTGGGCTTGAACCAGCTGACAGCAATAGCGCCTGGTCTCAGCAAATTGCAGACCATGACGTGATTGTTGGAGTTTTGGACACTGGGATTTGGCCGGAGAGTGAGAGCTTCAACGATGCTGGGATGTCGCCGGTGCCGGCGCATTGGAAAGGGGAATGCGAAACAGGGCGAGACTTCACGAAACAGAATTGTAACAGAAAGATCGTTGGAGCTAGAATGTTCTACCATGGGTATGAAGCTGCAACTGGGAAATTTAACGAACAGTTGGAGTATAAATCTCCAAGGGATCAAGATGGGCATGGAACTCACACGGCAGCCACCGTCGCCGGCTCTCCGACAGCCGGCGCGAACCTCCTCGGTTATGCGTATGGGACGGCCAGAGGAATGGCGCCCGGTGCTAGAATAGCGGCTTACAAAGTCTGTTGGGCCGGCGGCTGTTTTAGCTCCGACATTTTGTCGGCTGTCGACAGAGCTGTGGCCGATGGAGTAAATGTTCTGTCCATCTCTTTGGGAGGTGGGGTCTCTTCTTACTATCGCGATAGCCTCTCCGTTGCAGCATTTGGTGCAATGGAGATGGGCGTGTTTGTGTCCTGTTCGGCCGGAAATGCAGGGCCGGACCCTGTCAGTCTCACAAACGTATCGCCATGGATAACCACTGTCGGTGCCAGCACAATGGACAGAGATTTCCCGGCCATTGTCAAGCTCGGCAATGGAAGAACAGTCACCGGCGTTTCACTTTACAAAGGAAGAATCACAATCCCAGAAAGCAAACAATTCCCAGTTGTATACATGGGGAGTAACTCAAGCAACCCGGATCCGAGCTCGCTGTGTTTAGAAGGAACATTAGATCCCCATTTCGTGGCCGGAAAAATCGTGATATGCGATCGTGGAATTAGCCCACGGGTCCAGAAGGGTGTGGTGGTGAAGAATGCAGGTGGGGTTGGGATGATTCTGGCGAACACGGCGGCGAATGGGGAAGAACTTGTTGCCGATTGCCATTTGGTACCTGCCGTCGCCGTCGGTGAAAGAGAAGGTAAAGCAATTAAGCTGTACGCATTGACGAATCGAAAACCGACGGCGACTTTAGGATTTTTAGGGACAAGATTGGGGGTAAGACCCTCGCCGGTGGTGGCGGCATTTTCATCCAGAGGACCAAATTTCCTCACACTTGAGATCCTCAAGCCCGATCTGGTGGCCCCTGGCGTTAACATCCTTGCCGCTTGGACGGGAAAAACAGGGCCATCGAGTTTGACGACGGATACAAGAAGAGTTAAATTCAACATTCTGTCAGGAACCTCAATGTCTTGCCCACACGTCAGTGGCGTAGCAGCTCTGATCAAATCAAAGCATCCCGATTGGAGCCCGTCGGCAATCAAATCGGCACTGATGACCACCGCTTACGTCCACGACAACACCTATAAGCCTCTCAAAGACTCCTCCGGTGCATCGCCGTCGAGTCCGTACGACCACGGCGCCGGCCACATAAACCCAAGAAAAGCCCTAGATCCTGGCTTGGTTTACGAAATTCAGCCGCAAGATTACTTCGAATTCCTCTGTACGCAGGATTTAACCCCTTCACAGCTCAAAGTATTCTCCAAATACTCAAACAGATCTTGTCATCACCTCCTCCCCAACCCTGGAGACTTAAATTATCCGGCCATCTCTGCCGTTTTCCCCGAAAAAACCTCCGTCACTTCCTTGACCCTTCACAGAACCGTCACCAACGTCGGCCCTGCAACCTCCAGTTACCATGCCGTGGTGACTCCGTTCAACGGCGCCGCCGTGAAAGTTGAGCCGGAGAGTCTGAACTTCACTAGGAGATATCAGAAGCTTTCATACAAAATCACTTTCCTGACGAAGAAGAGACAGAGTATGCCGGAATTCGGAGGATTAATCTGGAAGGACGGAACTCATAGTGTGAGAAGTCCGATTGTGATCACTTGGTTGTCGTTTGTTTGA

Protein sequence

MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV

Homology

[Close]

BLAST of CmoCh16G001730 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 549/744 (73.79%), Postives = 644/744 (86.56%), Query Frame = 0


Query: 27  KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAAR 86
           KTYV+ MD+SAMP  +++HLQWYS+ + SV  +  +E   G   RI+Y+YQ  FHG+AA+
Sbjct: 35  KTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE-EEGNNNRILYTYQTAFHGLAAQ 94

Query: 87  LNEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVL 146
           L +EEAERLEEE+GV+A+ PET+YELHTTRSP FLGLE  +S   W++++ DHDV+VGVL
Sbjct: 95  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 154

Query: 147 DTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKIVGARMFYHGYEAATGKFN 206
           DTGIWPESESFND GMSPVPA W+G CETG+ F K+NCNRKIVGAR+FY GYEAATGK +
Sbjct: 155 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 214

Query: 207 EQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGC 266
           E+LEYKSPRD+DGHGTHTAATVAGSP  GANL G+AYGTARGMA  AR+AAYKVCW GGC
Sbjct: 215 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 274

Query: 267 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPD 326
           FSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGN GPD
Sbjct: 275 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 334

Query: 327 PVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPESKQFPVVYMGS 386
           P+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLYKGR  +P++KQ+P+VY+G 
Sbjct: 335 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 394

Query: 387 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 446
           N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGG+GM+L NTA NGE
Sbjct: 395 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 454

Query: 447 ELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATLGFLGTRLGVRPSPVVAAFSSRGPN 506
           ELVAD H++PAVAVGE+EGK IK YA+T++K TA+L  LGTR+G++PSPVVAAFSSRGPN
Sbjct: 455 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 514

Query: 507 FLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALI 566
           FL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVAALI
Sbjct: 515 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 574

Query: 567 KSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKALDPGLV 626
           KS+HPDWSP+AIKSALMTTAYVHDN +KPL D+SGA+PSSPYDHGAGHI+P +A DPGLV
Sbjct: 575 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 634

Query: 627 YEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHH-LLPNPGDLNYPAISAVFPEKTSVT 686
           Y+I PQ+YFEFLCTQDL+PSQLKVF+K+SNR+C H L  NPG+LNYPAISA+FPE T V 
Sbjct: 635 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 694

Query: 687 SLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYQKLSYKITFLTKKRQSM 746
           ++TL RTVTNVGP  SSY   V+PF GA+V V+P++LNFT ++QKLSY +TF T+ R   
Sbjct: 695 AMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR 754

Query: 747 PEFGGLIWKDGTHSVRSPIVITWL 770
           PEFGGL+WK  TH VRSP++ITWL
Sbjct: 755 PEFGGLVWKSTTHKVRSPVIITWL 777

[Close]

BLAST of CmoCh16G001730 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.9e-233
Identity = 412/760 (54.21%), Postives = 529/760 (69.61%), Query Frame = 0


Query: 11  LLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQ 70
           LL +  C + SS+    TY+V M +S MP SF  H  WY + L S+  + E         
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE--------- 73

Query: 71  RIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADSNS 130
            ++Y+Y+N  HG + RL +EEA+ L  + GV+++ PE +YELHTTR+P FLGL+   ++ 
Sbjct: 74  -LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD- 133

Query: 131 AWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKIVG 190
            + +  +  DV+VGVLDTG+WPES+S++D G  P+P+ WKG CE G +FT   CNRK++G
Sbjct: 134 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 193

Query: 191 ARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMA 250
           AR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS   GA+LLGYA GTARGMA
Sbjct: 194 ARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 253

Query: 251 PGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 310
           P AR+A YKVCW GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AM
Sbjct: 254 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAM 313

Query: 311 EMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGR 370
           E G+ VSCSAGNAGP   SL+NV+PWITTVGA T+DRDFPA+  LGNG+  TGVSL+KG 
Sbjct: 314 ERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE 373

Query: 371 ITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 430
             +P+ K  P +Y G N+SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK 
Sbjct: 374 -ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 433

Query: 431 AGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATLGFLGTRLG 490
           AGGVGMILANTAANGEELVAD HL+PA  VGE+ G  I+ Y  T+  PTA++  LGT +G
Sbjct: 434 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 493

Query: 491 VRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 550
           V+PSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+S
Sbjct: 494 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 553

Query: 551 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDH 610
           GTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+DH
Sbjct: 554 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 613

Query: 611 GAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNPGDLN 670
           GAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+ +         +  DLN
Sbjct: 614 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLN 673

Query: 671 YPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYQK 730
           YP+ +        V +    RTVT+VG A +    V +   G  + VEP  LNF    +K
Sbjct: 674 YPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 733

Query: 731 LSYKITFL--TKKRQSMPEFGGLIWKDGTHSVRSPIVITW 769
            SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 734 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

[Close]

BLAST of CmoCh16G001730 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 1.6e-220
Identity = 395/768 (51.43%), Postives = 519/768 (67.58%), Query Frame = 0


Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           + ++  F  LLL +    KTY+++++ S  P+SF  H  WY++ L S             
Sbjct: 11  ITIITTFLFLLLHTTA-KKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------- 70

Query: 69  EQRIIYSYQNVFHGVAARLNEEEAER-LEEENGVLAIFPETKYELHTTRSPRFLGLEPAD 128
           E  ++Y+Y   FHG +A L+  EA+  L   N +L IF +  Y LHTTR+P FLGL    
Sbjct: 71  ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--S 130

Query: 129 SNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRK 188
                    + + VI+GVLDTG+WPES SF+D  M  +P+ WKGECE+G DF  + CN+K
Sbjct: 131 EFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKK 190

Query: 189 IVGARMFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTA 248
           ++GAR F  G++ A+ G F+ + E  SPRD DGHGTHT+ T AGS    A+ LGYA GTA
Sbjct: 191 LIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTA 250

Query: 249 RGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAA 308
           RGMA  AR+A YKVCW+ GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ A
Sbjct: 251 RGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGA 310

Query: 309 FGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSL 368
           F AME GVFVSCSAGN+GP   S+ NV+PW+ TVGA T+DRDFPA   LGNG+ +TGVSL
Sbjct: 311 FSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSL 370

Query: 369 YKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGV 428
           Y G      +K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG 
Sbjct: 371 YSG--VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 430

Query: 429 VVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATLGFLG 488
           VV++AGG+GMI+ANTAA+GEELVAD HL+PA+AVG++ G  ++ Y  ++ KPTA L F G
Sbjct: 431 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKG 490

Query: 489 TRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKF 548
           T L V+PSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +F
Sbjct: 491 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 550

Query: 549 NILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSS 608
           NI+SGTSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+
Sbjct: 551 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 610

Query: 609 PYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNP 668
           PY HG+GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C     +P
Sbjct: 611 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP 670

Query: 669 GDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTR 728
           G LNYP+ S +F  K  V      R VTNVG A+S Y   V       + V+P  L+F  
Sbjct: 671 GQLNYPSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 730

Query: 729 RYQKLSYKITFLTKKRQSM---PEFGGLIWKDGTHSVRSPIVITWLSF 772
             +K  Y +TF++KK  SM    EFG + W +  H VRSP+  +W  F
Sbjct: 731 VGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

[Close]

BLAST of CmoCh16G001730 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.4e-216
Identity = 384/758 (50.66%), Postives = 512/758 (67.55%), Query Frame = 0


Query: 28  TYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAARL 87
           TY+V +D  A P  F  H  WY++ L S+  +P           II++Y  VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  NEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
             ++A +L +   V+++ PE    LHTTRSP FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   +DF +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 QLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGCF 267
             E++SPRD DGHGTHTA+  AG     A+ LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGN GP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPESKQFPVVYMGS- 387
           +++TNV+PW+TTVGA T+DRDFPA VKLGNG+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 447
              +   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI+AN   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAVGEREGKAIKLY------ALTNRKPTATLGFLGTRLGVRPSPVVAAF 507
            LVADCH++PA +VG   G  I+ Y      + +++ PTAT+ F GTRLG+RP+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKY-----SNRSCHHLLPNPGDLNYPAIS 687
           +DPGLVY+I   DY  FLC  + T + +   ++        R   H+    G+LNYP+ S
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV----GNLNYPSFS 686

Query: 688 AVFPE-KTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYQKLSYK 747
            VF +   S  S    RTVTNVG + S Y   + P  G  V VEPE L+F R  QKLS+ 
Sbjct: 687 VVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFV 746

Query: 748 ITFLTKKRQSMP-----EFGGLIWKDGTHSVRSPIVIT 768
           +   T + +  P     E G ++W DG  +V SP+V+T
Sbjct: 747 VRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

[Close]

BLAST of CmoCh16G001730 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 1.1e-213
Identity = 391/772 (50.65%), Postives = 514/772 (66.58%), Query Frame = 0


Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           L L L F  +  +++Q  KT++ ++D  +MP  F  H  WYST                 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  EQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADS 128
           E RI++ Y  VFHG +A +  +EA+ L     VLA+F + + ELHTTRSP+FLGL+  + 
Sbjct: 68  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQ 127

Query: 129 NSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKI 188
              WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNRKI
Sbjct: 128 KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 187

Query: 189 VGARMFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTAR 248
           +GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     A++ GYA G A+
Sbjct: 188 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 247

Query: 249 GMAPGARIAAYKVCWA-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLS 308
           G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D ++
Sbjct: 248 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 307

Query: 309 VAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTG 368
           + ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LG+G  + G
Sbjct: 308 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 367

Query: 369 VSLYKGRITIP-ESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRV 428
           VSLY G   +P   + FPVVY G   S    +SLC+E TLDP  V GKIVICDRG SPRV
Sbjct: 368 VSLYAG---VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 427

Query: 429 QKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATL 488
            KG+VVK AGGVGMILAN A+NGE LV D HL+PA AVG  EG  IK YA ++  P A++
Sbjct: 428 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 487

Query: 489 GFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTR 548
            F GT +G++P+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D R
Sbjct: 488 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 547

Query: 549 RVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGA 608
           + +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S  
Sbjct: 548 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 607

Query: 609 SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHL 668
             ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R     
Sbjct: 608 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTR 667

Query: 669 LPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPES 728
            P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G  V V+P  
Sbjct: 668 KPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPR 727

Query: 729 LNFTRRYQKLSYKITFLTKKR-----QSMPEFGGLIWKD-GTHSVRSPIVIT 768
           L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

[Close]

BLAST of CmoCh16G001730 vs. ExPASy TrEMBL
Match: A0A6J1E4V2 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE=3 SV=1)

HSP 1 Score: 1559.3 bits (4036), Expect = 0.0e+00
Identity = 772/772 (100.00%), Postives = 772/772 (100.00%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 773
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]

BLAST of CmoCh16G001730 vs. ExPASy TrEMBL
Match: A0A6J1J576 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3 SV=1)

HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 757/772 (98.06%), Postives = 766/772 (99.22%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDC LLSSAQF+KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARL+EEEAERLEEE+GVLAIFPETKY+LHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSPTA ANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIK+YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           G SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVGPATSSYHAV TPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 773
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]

BLAST of CmoCh16G001730 vs. ExPASy TrEMBL
Match: A0A6J1FZU3 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE=3 SV=1)

HSP 1 Score: 1458.4 bits (3774), Expect = 0.0e+00
Identity = 715/773 (92.50%), Postives = 747/773 (96.64%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVV-N 60
           MAHTPL+WLC  L F CL  SSAQF KTYVVQMDRSAMPDSFSDHL+WYSTV++SVVV N
Sbjct: 1   MAHTPLQWLCFPLFFHCLFFSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PERE--GNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRS 120
           PERE  GNGGGE RIIYSYQNVFHGVAARL+EEEAERLEEE+GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPA+WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 DFTKQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGAN 240
            FTKQNCNRKIVGAR+FYHGYEAATGKFN+QLEYKSPRDQDGHGTHTAATVAGSP AGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTVTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLY+GRITI E+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRK 480
           ISPRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHL+PAVAVGE EGKAIK YALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLG LGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNR 660
           D+S ASPSSPY+HGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTPSQLKVFSK+SNR
Sbjct: 601 DASAASPSSPYEHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKV 720
           SCH LLPNPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVGPA+SSYHA+V+PF GAAVK+
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLS 771
           EPE LNFTRRYQKLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPI+ITWLS
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773

[Close]

BLAST of CmoCh16G001730 vs. ExPASy TrEMBL
Match: A0A6J1HS88 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111467351 PE=3 SV=1)

HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 714/773 (92.37%), Postives = 745/773 (96.38%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVV-N 60
           MAHTPL+WLCL L F C LLSSAQF  TYVVQMDRSAMPDSFSD  +WYSTVL+SVVV N
Sbjct: 1   MAHTPLQWLCLPLFFHCFLLSSAQFFNTYVVQMDRSAMPDSFSDPFEWYSTVLSSVVVDN 60

Query: 61  PERE--GNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRS 120
           PERE  GNGGGE RIIYSYQNVFHGVAARL+EEEAER+EEE+GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERVEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPA+WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 DFTKQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGAN 240
            FTKQNCNRKIVGAR+FYHGYEAATGKFN+QLEYKSPRDQDGHGTHTAATVAGSP AGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTVTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLY+GRITI E+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRK 480
           ISPRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHL+PAVAVGE EGKAIK YALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATL  LGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLEILGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLR 600

Query: 601 DSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNR 660
           D+S ASPSSPYDHGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTPSQLKVFSK+SNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKV 720
           SC+ LL NPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVGPA+SSYHA+V+PFNGAAVKV
Sbjct: 661 SCNRLLLNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFNGAAVKV 720

Query: 721 EPESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLS 771
           EPE LNFTRRYQKLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPI+ITWLS
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773

[Close]

BLAST of CmoCh16G001730 vs. ExPASy TrEMBL
Match: A0A5A7SUB4 (Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00040 PE=3 SV=1)

HSP 1 Score: 1437.2 bits (3719), Expect = 0.0e+00
Identity = 706/773 (91.33%), Postives = 746/773 (96.51%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MA +P+K LCLLL  D LLLSSA F+KTYVVQMDRSAMP SF++H +WYS VL++VVV+ 
Sbjct: 1   MADSPIKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  EREGN-GGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPR 120
           EREGN GGGE+RIIYSYQNVFHGVAARL+EEE E+LEEE+GV+AIFPE KYELHTTRSPR
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVPAHWKGECETGR F
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLL 240
           TKQNCNRKIVGAR+FYHGY+AATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP AGA+LL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGR 360
           DSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GR
Sbjct: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360

Query: 361 TVTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLY+GRITIPE+KQFP+VYMGSNSS+PDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 361 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 420

Query: 421 PRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPT 480
           PRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRK T
Sbjct: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480

Query: 481 ATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+DS
Sbjct: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 600

Query: 601 SGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSC 660
           S ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYF+FLCTQDLTP+QLKVFSKYSNR+C
Sbjct: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660

Query: 661 HHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEP 720
             LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPF GAAVKVEP
Sbjct: 661 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 720

Query: 721 ESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 773
           ESLNFTRRY+KLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773

[Close]

BLAST of CmoCh16G001730 vs. NCBI nr
Match: XP_022922929.1 (subtilisin-like protease SBT1.3 [Cucurbita moschata])

HSP 1 Score: 1559.3 bits (4036), Expect = 0.0e+00
Identity = 772/772 (100.00%), Postives = 772/772 (100.00%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 773
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]

BLAST of CmoCh16G001730 vs. NCBI nr
Match: KAG6576764.1 (Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1548.5 bits (4008), Expect = 0.0e+00
Identity = 764/772 (98.96%), Postives = 771/772 (99.87%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           M HTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MGHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARL+E+EAERLEEE+GVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIK+YALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 773
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]

BLAST of CmoCh16G001730 vs. NCBI nr
Match: XP_023552616.1 (subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1548.1 bits (4007), Expect = 0.0e+00
Identity = 765/772 (99.09%), Postives = 770/772 (99.74%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARL+EEEAERLEEE+GVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIK+YALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHA VTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 773
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]

BLAST of CmoCh16G001730 vs. NCBI nr
Match: XP_022984431.1 (subtilisin-like protease SBT1.3 [Cucurbita maxima])

HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 757/772 (98.06%), Postives = 766/772 (99.22%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDC LLSSAQF+KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARL+EEEAERLEEE+GVLAIFPETKY+LHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSPTA ANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIK+YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           G SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVGPATSSYHAV TPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 773
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

[Close]

BLAST of CmoCh16G001730 vs. NCBI nr
Match: KAG7014803.1 (Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 752/767 (98.04%), Postives = 761/767 (99.22%), Query Frame = 0


Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLL SSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLHSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARL+E+EAERLEEE+GVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGA+MFYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGAKMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGR TIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRTTIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIK+YALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVIT 768
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVR    +T
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRITFAVT 767

[Close]

BLAST of CmoCh16G001730 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 549/744 (73.79%), Postives = 644/744 (86.56%), Query Frame = 0


Query: 27  KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAAR 86
           KTYV+ MD+SAMP  +++HLQWYS+ + SV  +  +E   G   RI+Y+YQ  FHG+AA+
Sbjct: 35  KTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE-EEGNNNRILYTYQTAFHGLAAQ 94

Query: 87  LNEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVL 146
           L +EEAERLEEE+GV+A+ PET+YELHTTRSP FLGLE  +S   W++++ DHDV+VGVL
Sbjct: 95  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 154

Query: 147 DTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKIVGARMFYHGYEAATGKFN 206
           DTGIWPESESFND GMSPVPA W+G CETG+ F K+NCNRKIVGAR+FY GYEAATGK +
Sbjct: 155 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 214

Query: 207 EQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGC 266
           E+LEYKSPRD+DGHGTHTAATVAGSP  GANL G+AYGTARGMA  AR+AAYKVCW GGC
Sbjct: 215 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 274

Query: 267 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPD 326
           FSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGN GPD
Sbjct: 275 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 334

Query: 327 PVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPESKQFPVVYMGS 386
           P+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLYKGR  +P++KQ+P+VY+G 
Sbjct: 335 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 394

Query: 387 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 446
           N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGG+GM+L NTA NGE
Sbjct: 395 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 454

Query: 447 ELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATLGFLGTRLGVRPSPVVAAFSSRGPN 506
           ELVAD H++PAVAVGE+EGK IK YA+T++K TA+L  LGTR+G++PSPVVAAFSSRGPN
Sbjct: 455 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 514

Query: 507 FLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALI 566
           FL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVAALI
Sbjct: 515 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 574

Query: 567 KSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKALDPGLV 626
           KS+HPDWSP+AIKSALMTTAYVHDN +KPL D+SGA+PSSPYDHGAGHI+P +A DPGLV
Sbjct: 575 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 634

Query: 627 YEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHH-LLPNPGDLNYPAISAVFPEKTSVT 686
           Y+I PQ+YFEFLCTQDL+PSQLKVF+K+SNR+C H L  NPG+LNYPAISA+FPE T V 
Sbjct: 635 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 694

Query: 687 SLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYQKLSYKITFLTKKRQSM 746
           ++TL RTVTNVGP  SSY   V+PF GA+V V+P++LNFT ++QKLSY +TF T+ R   
Sbjct: 695 AMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR 754

Query: 747 PEFGGLIWKDGTHSVRSPIVITWL 770
           PEFGGL+WK  TH VRSP++ITWL
Sbjct: 755 PEFGGLVWKSTTHKVRSPVIITWL 777

[Close]

BLAST of CmoCh16G001730 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 809.7 bits (2090), Expect = 2.1e-234
Identity = 412/760 (54.21%), Postives = 529/760 (69.61%), Query Frame = 0


Query: 11  LLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQ 70
           LL +  C + SS+    TY+V M +S MP SF  H  WY + L S+  + E         
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE--------- 73

Query: 71  RIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADSNS 130
            ++Y+Y+N  HG + RL +EEA+ L  + GV+++ PE +YELHTTR+P FLGL+   ++ 
Sbjct: 74  -LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD- 133

Query: 131 AWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKIVG 190
            + +  +  DV+VGVLDTG+WPES+S++D G  P+P+ WKG CE G +FT   CNRK++G
Sbjct: 134 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 193

Query: 191 ARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMA 250
           AR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS   GA+LLGYA GTARGMA
Sbjct: 194 ARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 253

Query: 251 PGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 310
           P AR+A YKVCW GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AM
Sbjct: 254 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAM 313

Query: 311 EMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGR 370
           E G+ VSCSAGNAGP   SL+NV+PWITTVGA T+DRDFPA+  LGNG+  TGVSL+KG 
Sbjct: 314 ERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE 373

Query: 371 ITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 430
             +P+ K  P +Y G N+SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK 
Sbjct: 374 -ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 433

Query: 431 AGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATLGFLGTRLG 490
           AGGVGMILANTAANGEELVAD HL+PA  VGE+ G  I+ Y  T+  PTA++  LGT +G
Sbjct: 434 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 493

Query: 491 VRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 550
           V+PSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+S
Sbjct: 494 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 553

Query: 551 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDH 610
           GTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+DH
Sbjct: 554 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 613

Query: 611 GAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNPGDLN 670
           GAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+ +         +  DLN
Sbjct: 614 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLN 673

Query: 671 YPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYQK 730
           YP+ +        V +    RTVT+VG A +    V +   G  + VEP  LNF    +K
Sbjct: 674 YPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 733

Query: 731 LSYKITFL--TKKRQSMPEFGGLIWKDGTHSVRSPIVITW 769
            SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 734 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

[Close]

BLAST of CmoCh16G001730 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 767.3 bits (1980), Expect = 1.2e-221
Identity = 395/768 (51.43%), Postives = 519/768 (67.58%), Query Frame = 0


Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           + ++  F  LLL +    KTY+++++ S  P+SF  H  WY++ L S             
Sbjct: 11  ITIITTFLFLLLHTTA-KKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------- 70

Query: 69  EQRIIYSYQNVFHGVAARLNEEEAER-LEEENGVLAIFPETKYELHTTRSPRFLGLEPAD 128
           E  ++Y+Y   FHG +A L+  EA+  L   N +L IF +  Y LHTTR+P FLGL    
Sbjct: 71  ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--S 130

Query: 129 SNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRK 188
                    + + VI+GVLDTG+WPES SF+D  M  +P+ WKGECE+G DF  + CN+K
Sbjct: 131 EFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKK 190

Query: 189 IVGARMFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTA 248
           ++GAR F  G++ A+ G F+ + E  SPRD DGHGTHT+ T AGS    A+ LGYA GTA
Sbjct: 191 LIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTA 250

Query: 249 RGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAA 308
           RGMA  AR+A YKVCW+ GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ A
Sbjct: 251 RGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGA 310

Query: 309 FGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSL 368
           F AME GVFVSCSAGN+GP   S+ NV+PW+ TVGA T+DRDFPA   LGNG+ +TGVSL
Sbjct: 311 FSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSL 370

Query: 369 YKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGV 428
           Y G      +K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG 
Sbjct: 371 YSG--VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 430

Query: 429 VVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATLGFLG 488
           VV++AGG+GMI+ANTAA+GEELVAD HL+PA+AVG++ G  ++ Y  ++ KPTA L F G
Sbjct: 431 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKG 490

Query: 489 TRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKF 548
           T L V+PSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +F
Sbjct: 491 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 550

Query: 549 NILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSS 608
           NI+SGTSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+
Sbjct: 551 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 610

Query: 609 PYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNP 668
           PY HG+GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C     +P
Sbjct: 611 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP 670

Query: 669 GDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTR 728
           G LNYP+ S +F  K  V      R VTNVG A+S Y   V       + V+P  L+F  
Sbjct: 671 GQLNYPSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 730

Query: 729 RYQKLSYKITFLTKKRQSM---PEFGGLIWKDGTHSVRSPIVITWLSF 772
             +K  Y +TF++KK  SM    EFG + W +  H VRSP+  +W  F
Sbjct: 731 VGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

[Close]

BLAST of CmoCh16G001730 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 754.2 bits (1946), Expect = 1.0e-217
Identity = 384/758 (50.66%), Postives = 512/758 (67.55%), Query Frame = 0


Query: 28  TYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAARL 87
           TY+V +D  A P  F  H  WY++ L S+  +P           II++Y  VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  NEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
             ++A +L +   V+++ PE    LHTTRSP FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   +DF +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 QLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGCF 267
             E++SPRD DGHGTHTA+  AG     A+ LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGN GP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPESKQFPVVYMGS- 387
           +++TNV+PW+TTVGA T+DRDFPA VKLGNG+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 447
              +   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI+AN   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAVGEREGKAIKLY------ALTNRKPTATLGFLGTRLGVRPSPVVAAF 507
            LVADCH++PA +VG   G  I+ Y      + +++ PTAT+ F GTRLG+RP+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKY-----SNRSCHHLLPNPGDLNYPAIS 687
           +DPGLVY+I   DY  FLC  + T + +   ++        R   H+    G+LNYP+ S
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV----GNLNYPSFS 686

Query: 688 AVFPE-KTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYQKLSYK 747
            VF +   S  S    RTVTNVG + S Y   + P  G  V VEPE L+F R  QKLS+ 
Sbjct: 687 VVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFV 746

Query: 748 ITFLTKKRQSMP-----EFGGLIWKDGTHSVRSPIVIT 768
           +   T + +  P     E G ++W DG  +V SP+V+T
Sbjct: 747 VRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

[Close]

BLAST of CmoCh16G001730 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 744.6 bits (1921), Expect = 8.1e-215
Identity = 391/772 (50.65%), Postives = 514/772 (66.58%), Query Frame = 0


Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           L L L F  +  +++Q  KT++ ++D  +MP  F  H  WYST                 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  EQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRFLGLEPADS 128
           E RI++ Y  VFHG +A +  +EA+ L     VLA+F + + ELHTTRSP+FLGL+  + 
Sbjct: 68  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQ 127

Query: 129 NSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFTKQNCNRKI 188
              WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNRKI
Sbjct: 128 KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 187

Query: 189 VGARMFYHGYEAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTAR 248
           +GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     A++ GYA G A+
Sbjct: 188 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 247

Query: 249 GMAPGARIAAYKVCWA-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLS 308
           G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D ++
Sbjct: 248 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 307

Query: 309 VAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTG 368
           + ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LG+G  + G
Sbjct: 308 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 367

Query: 369 VSLYKGRITIP-ESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRV 428
           VSLY G   +P   + FPVVY G   S    +SLC+E TLDP  V GKIVICDRG SPRV
Sbjct: 368 VSLYAG---VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 427

Query: 429 QKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTATL 488
            KG+VVK AGGVGMILAN A+NGE LV D HL+PA AVG  EG  IK YA ++  P A++
Sbjct: 428 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 487

Query: 489 GFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTR 548
            F GT +G++P+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D R
Sbjct: 488 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 547

Query: 549 RVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGA 608
           + +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S  
Sbjct: 548 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 607

Query: 609 SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHL 668
             ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R     
Sbjct: 608 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTR 667

Query: 669 LPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPES 728
            P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G  V V+P  
Sbjct: 668 KPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPR 727

Query: 729 LNFTRRYQKLSYKITFLTKKR-----QSMPEFGGLIWKD-GTHSVRSPIVIT 768
           L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

The following BLAST results are available for this feature:

BLAST of CmoCh16G001730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLI4	0.0e+00	73.79	Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S...	[more]
O65351	2.9e-233	54.21	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
Q9ZUF6	1.6e-220	51.43	Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S...	[more]
Q9LUM3	1.4e-216	50.66	Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S...	[more]
O49607	1.1e-213	50.65	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]

BLAST of CmoCh16G001730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E4V2	0.0e+00	100.00	subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE...	[more]
A0A6J1J576	0.0e+00	98.06	subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3...	[more]
A0A6J1FZU3	0.0e+00	92.50	subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE...	[more]
A0A6J1HS88	0.0e+00	92.37	subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111467351 PE=3...	[more]
A0A5A7SUB4	0.0e+00	91.33	Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of CmoCh16G001730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922929.1	0.0e+00	100.00	subtilisin-like protease SBT1.3 [Cucurbita moschata]	[more]
KAG6576764.1	0.0e+00	98.96	Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023552616.1	0.0e+00	99.09	subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo]	[more]
XP_022984431.1	0.0e+00	98.06	subtilisin-like protease SBT1.3 [Cucurbita maxima]	[more]
KAG7014803.1	0.0e+00	98.04	Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of CmoCh16G001730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51750.1	0.0e+00	73.79	subtilase 1.3	[more]
AT5G67360.1	2.1e-234	54.21	Subtilase family protein	[more]
AT2G05920.1	1.2e-221	51.43	Subtilase family protein	[more]
AT3G14240.1	1.0e-217	50.66	Subtilase family protein	[more]
AT4G34980.1	8.1e-215	50.65	subtilisin-like serine protease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 216..229 score: 50.79 coord: 550..566 score: 58.72 coord: 138..157 score: 31.6
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 668..766 e-value: 3.2E-27 score: 94.6
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 139..592 e-value: 3.1E-53 score: 181.3
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 140..634 e-value: 7.9E-185 score: 617.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 112..625
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 19..113 e-value: 8.2E-20 score: 73.0
None	No IPR available	GENE3D	2.60.40.2310		coord: 638..769 e-value: 1.5E-34 score: 120.5
None	No IPR available	GENE3D	3.50.30.30		coord: 346..490 e-value: 7.9E-185 score: 617.0
None	No IPR available	PANTHER	PTHR10795:SF675	SUBTILISIN-LIKE PROTEASE SBT1.7	coord: 18..767
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 109..621 score: 31.217665
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 353..482 e-value: 5.44014E-39 score: 138.701
IPR003137	PA domain	PFAM	PF02225	PA	coord: 380..468 e-value: 1.5E-9 score: 37.9
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 28..113 e-value: 3.5E-16 score: 59.6
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 18..767
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 551..561
IPR034197	Cucumisin-like catalytic domain	CDD	cd04852	Peptidases_S8_3	coord: 110..587 e-value: 3.12322E-153 score: 446.66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh16G001730.1	CmoCh16G001730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity