CmoCh16G001580 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G001580
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptiondehydration-responsive element-binding protein 1A-like
LocationCmo_Chr16: 718294 .. 718929 (+)
RNA-Seq ExpressionCmoCh16G001580
SyntenyCmoCh16G001580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCTTTTTCAACTTTTTATGAAGACTACTTCTCCAGCTCCGAGAGTAGTACTTGCCGGCGGACCATATTTTCCGAGAAGGAGCTTATGTTAGCGGCGAGCAATCCCAAGAAGAGGGCCGGCAGGAAGAAGTTTAAGGAGACAAGGCACCCTGTTTACAGAGGGGTCAGGAGGAGGAACTCCGGCAAGTGGGTCTGTGAAGTGAGAGAGCCTAATAAGAAGTCAAGGATTTGGCTTGGCACTTTTCCGACGGCGGAGATGGCGGCTCGGGCTCACGACGTCGCTGCCATGAAGCTTCGAGGTCGCTCTGCTTGTCTCAATTTTGCAGATTCTGCGTGGCGGCTGCCGGAGCCAGCATCTGCTGACGCCAGGGATATTCAGAAGGCGGCGGCGGAGGCGGCGGAGGCATTCCGGCCGGCCGAATCAGATGGGTCTTCTGTTGATGACTCAAGGACGGAAAATATGATGACGGCGGAGACGACGCCAGAAAACGTGTTGTACGTGGATGATGATGCAATTTTTGGGCTGCCGGGATTGTTGGAGGATATGGCTGCAGGGATGATGTTGCCTCCACCTCAGCATTTTCGTGATGACATGGATTTTTATTCTGACGTGTCATTATGGAGCTACTAA

mRNA sequence

ATGGAATCTTTTTCAACTTTTTATGAAGACTACTTCTCCAGCTCCGAGAGTAGTACTTGCCGGCGGACCATATTTTCCGAGAAGGAGCTTATGTTAGCGGCGAGCAATCCCAAGAAGAGGGCCGGCAGGAAGAAGTTTAAGGAGACAAGGCACCCTGTTTACAGAGGGGTCAGGAGGAGGAACTCCGGCAAGTGGGTCTGTGAAGTGAGAGAGCCTAATAAGAAGTCAAGGATTTGGCTTGGCACTTTTCCGACGGCGGAGATGGCGGCTCGGGCTCACGACGTCGCTGCCATGAAGCTTCGAGGTCGCTCTGCTTGTCTCAATTTTGCAGATTCTGCGTGGCGGCTGCCGGAGCCAGCATCTGCTGACGCCAGGGATATTCAGAAGGCGGCGGCGGAGGCGGCGGAGGCATTCCGGCCGGCCGAATCAGATGGGTCTTCTGTTGATGACTCAAGGACGGAAAATATGATGACGGCGGAGACGACGCCAGAAAACGTGTTGTACGTGGATGATGATGCAATTTTTGGGCTGCCGGGATTGTTGGAGGATATGGCTGCAGGGATGATGTTGCCTCCACCTCAGCATTTTCGTGATGACATGGATTTTTATTCTGACGTGTCATTATGGAGCTACTAA

Coding sequence (CDS)

ATGGAATCTTTTTCAACTTTTTATGAAGACTACTTCTCCAGCTCCGAGAGTAGTACTTGCCGGCGGACCATATTTTCCGAGAAGGAGCTTATGTTAGCGGCGAGCAATCCCAAGAAGAGGGCCGGCAGGAAGAAGTTTAAGGAGACAAGGCACCCTGTTTACAGAGGGGTCAGGAGGAGGAACTCCGGCAAGTGGGTCTGTGAAGTGAGAGAGCCTAATAAGAAGTCAAGGATTTGGCTTGGCACTTTTCCGACGGCGGAGATGGCGGCTCGGGCTCACGACGTCGCTGCCATGAAGCTTCGAGGTCGCTCTGCTTGTCTCAATTTTGCAGATTCTGCGTGGCGGCTGCCGGAGCCAGCATCTGCTGACGCCAGGGATATTCAGAAGGCGGCGGCGGAGGCGGCGGAGGCATTCCGGCCGGCCGAATCAGATGGGTCTTCTGTTGATGACTCAAGGACGGAAAATATGATGACGGCGGAGACGACGCCAGAAAACGTGTTGTACGTGGATGATGATGCAATTTTTGGGCTGCCGGGATTGTTGGAGGATATGGCTGCAGGGATGATGTTGCCTCCACCTCAGCATTTTCGTGATGACATGGATTTTTATTCTGACGTGTCATTATGGAGCTACTAA

Protein sequence

MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRRNSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPASADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGLLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Homology
BLAST of CmoCh16G001580 vs. ExPASy Swiss-Prot
Match: Q9M0L0 (Dehydration-responsive element-binding protein 1A OS=Arabidopsis thaliana OX=3702 GN=DREB1A PE=1 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 7.0e-57
Identity = 133/222 (59.91%), Postives = 154/222 (69.37%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M SFS F E + S  ESS      +      LA+S PKK AGRKKF+ETRHP+YRGVRRR
Sbjct: 1   MNSFSAFSEMFGSDYESSVSSGGDYIP---TLASSCPKKPAGRKKFRETRHPIYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKK+RIWLGTF TAEMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVCEVREPNKKTRIWLGTFQTAEMAARAHDVAALALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFRPAESD-----GSSVDDSRTENMMTAETTPENVLYVDDDAIF 180
           S  A+DIQKAAAEAA AF+    D     G  ++++  E + TAE + EN  Y+ D+A+F
Sbjct: 121 STCAKDIQKAAAEAALAFQDEMCDATTDHGFDMEETLVEAIYTAEQS-ENAFYMHDEAMF 180

Query: 181 GLPGLLEDMAAGMMLPPP------QHFRDDMDFYSDVSLWSY 212
            +P LL +MA GM+LP P       H  D  D   DVSLWSY
Sbjct: 181 EMPSLLANMAEGMLLPLPSVQWNHNHEVDGDD--DDVSLWSY 216

BLAST of CmoCh16G001580 vs. ExPASy Swiss-Prot
Match: Q9FJ93 (Dehydration-responsive element-binding protein 1D OS=Arabidopsis thaliana OX=3702 GN=DREB1D PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 4.6e-56
Identity = 125/223 (56.05%), Postives = 152/223 (68.16%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M  F + + D F S        +  SE    LA+S PKKRAGRKKF+ETRHP+YRGVR+R
Sbjct: 1   MNPFYSTFPDSFLSISDHRSPVSDSSECSPKLASSCPKKRAGRKKFRETRHPIYRGVRQR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPT EMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTVEMAARAHDVAALALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFR----------PAESDGSSVDDSRTENMMTAETTPENVLYVD 180
           +   ++IQKAA+EAA AF+           AE++ ++ +  R E    AE     V Y+D
Sbjct: 121 TTCPKEIQKAASEAAMAFQNETTTEGSKTAAEAEEAAGEGVR-EGERRAEEQNGGVFYMD 180

Query: 181 DDAIFGLPGLLEDMAAGMMLPPPQ--HFRDDMDFYSDVSLWSY 212
           D+A+ G+P   E+MA GM+LPPP+     +D D   DVSLWS+
Sbjct: 181 DEALLGMPNFFENMAEGMLLPPPEVGWNHNDFDGVGDVSLWSF 222

BLAST of CmoCh16G001580 vs. ExPASy Swiss-Prot
Match: Q9SYS6 (Dehydration-responsive element-binding protein 1C OS=Arabidopsis thaliana OX=3702 GN=DREB1C PE=1 SV=2)

HSP 1 Score: 216.1 bits (549), Expect = 3.9e-55
Identity = 123/220 (55.91%), Postives = 156/220 (70.91%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M SFS F E + S  ES       +S K   LA S PKK AGRKKF+ETRHP+YRGVR+R
Sbjct: 1   MNSFSAFSEMFGSDYESPVSSGGDYSPK---LATSCPKKPAGRKKFRETRHPIYRGVRQR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCE+REPNKK+RIWLGTF TAEMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVCELREPNKKTRIWLGTFQTAEMAARAHDVAAIALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFR------PAESDGSSVDDSRTENMMTAETTPENVLYVDDDAI 180
           S  A++IQKAAAEAA  F+        ++ G  ++++  E + T E + ++  Y+D++A+
Sbjct: 121 STCAKEIQKAAAEAALNFQDEMCHMTTDAHGLDMEETLVEAIYTPEQS-QDAFYMDEEAM 180

Query: 181 FGLPGLLEDMAAGMMLPPPQ---HFRDDMDFYSDVSLWSY 212
            G+  LL++MA GM+LP P    ++  D++   DVSLWSY
Sbjct: 181 LGMSSLLDNMAEGMLLPSPSVQWNYNFDVEGDDDVSLWSY 216

BLAST of CmoCh16G001580 vs. ExPASy Swiss-Prot
Match: P93835 (Dehydration-responsive element-binding protein 1B OS=Arabidopsis thaliana OX=3702 GN=DREB1B PE=1 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 8.6e-55
Identity = 126/220 (57.27%), Postives = 151/220 (68.64%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M SFS F E + S  E          +    LA S PKK AGRKKF+ETRHP+YRGVR+R
Sbjct: 1   MNSFSAFSEMFGSDYEPQG------GDYCPTLATSCPKKPAGRKKFRETRHPIYRGVRQR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWV EVREPNKK+RIWLGTF TAEMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVSEVREPNKKTRIWLGTFQTAEMAARAHDVAALALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFRPAESD------GSSVDDSRTENMMTAETTPENVLYVDDDAI 180
           S  A+DIQKAAAEAA AF+    D      G  ++++  E + T E + E   Y+D++ +
Sbjct: 121 STCAKDIQKAAAEAALAFQDETCDTTTTNHGLDMEETMVEAIYTPEQS-EGAFYMDEETM 180

Query: 181 FGLPGLLEDMAAGMMLPPPQ---HFRDDMDFYSDVSLWSY 212
           FG+P LL++MA GM+LPPP    +   D +   DVSLWSY
Sbjct: 181 FGMPTLLDNMAEGMLLPPPSVQWNHNYDGEGDGDVSLWSY 213

BLAST of CmoCh16G001580 vs. ExPASy Swiss-Prot
Match: Q9SGJ6 (Dehydration-responsive element-binding protein 1E OS=Arabidopsis thaliana OX=3702 GN=DREB1E PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.2e-43
Identity = 100/192 (52.08%), Postives = 128/192 (66.67%), Query Frame = 0

Query: 29  ELMLAASNPKKRAGRKKFKETRHPVYRGVRRRNSGKWVCEVREPNKKSRIWLGTFPTAEM 88
           ++ +A   PKKRAGR+ FKETRHP+YRGVRRR+  KWVCEVREP  + R+WLGT+PTA+M
Sbjct: 5   DITVAEMKPKKRAGRRIFKETRHPIYRGVRRRDGDKWVCEVREPIHQRRVWLGTYPTADM 64

Query: 89  AARAHDVAAMKLRGRSACLNFADSAWRLPEPASADARDIQKAAAEAAEAFRPAE-SDGSS 148
           AARAHDVA + LRGRSACLNF+DSAWRLP PAS D   I++ AAEAAE FRP E S G +
Sbjct: 65  AARAHDVAVLALRGRSACLNFSDSAWRLPVPASTDPDTIRRTAAEAAEMFRPPEFSTGIT 124

Query: 149 VDDSRTENMMTAETTPENVLYVDDDAIFGLPGLLEDMAAGMMLPPPQH--------FRDD 208
           V  S +E     +T+ E           G+ G++  +A   ++ PP+         + D+
Sbjct: 125 VLPSASE----FDTSDE-----------GVAGMMMRLAEEPLMSPPRSYIDMNTSVYVDE 181

Query: 209 MDFYSDVSLWSY 212
              Y D+SLWSY
Sbjct: 185 EMCYEDLSLWSY 181

BLAST of CmoCh16G001580 vs. ExPASy TrEMBL
Match: A0A6J1EAB5 (dehydration-responsive element-binding protein 1A-like OS=Cucurbita moschata OX=3662 GN=LOC111430798 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.1e-113
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180
           SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL
Sbjct: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180

Query: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 211

BLAST of CmoCh16G001580 vs. ExPASy TrEMBL
Match: A0A6J1J9I5 (dehydration-responsive element-binding protein 1A-like OS=Cucurbita maxima OX=3661 GN=LOC111482959 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.8e-111
Identity = 208/211 (98.58%), Postives = 208/211 (98.58%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           MESFST YEDYFSSSESS CRR IFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MESFSTLYEDYFSSSESSDCRRFIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180
           SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL
Sbjct: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180

Query: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 211

BLAST of CmoCh16G001580 vs. ExPASy TrEMBL
Match: A0A5D3DFF6 (Dehydration-responsive element-binding protein 1A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00300 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.7e-101
Identity = 189/212 (89.15%), Postives = 201/212 (94.81%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M+SFS FYE+YFSSSESS CRR IFS+++ MLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MDSFSNFYEEYFSSSESSNCRRPIFSDEDFMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAA+ LRG +ACLNFADSAWRLP PA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAIALRGLTACLNFADSAWRLPVPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAE-TTPENVLYVDDDAIFGLPG 180
           SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTEN M AE TTPENV Y+D++A+FG+PG
Sbjct: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENGMMAETTTPENVFYMDEEAVFGMPG 180

Query: 181 LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212

BLAST of CmoCh16G001580 vs. ExPASy TrEMBL
Match: A0A140DCV6 (CRT/DRE-binding factor 1 OS=Cucumis melo OX=3656 GN=LOC103484041 PE=2 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 188/212 (88.68%), Postives = 200/212 (94.34%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M+SFS FYE+YFSSSESS CRR IFS+++ MLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MDSFSNFYEEYFSSSESSNCRRPIFSDEDFMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAA+ LRG +ACLNFADSAWRLP PA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAIALRGLTACLNFADSAWRLPVPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAE-TTPENVLYVDDDAIFGLPG 180
           S DARDIQKAAAEAAEAFRPAESDGSSVDDSRTEN M AE TTPENV Y+D++A+FG+PG
Sbjct: 121 STDARDIQKAAAEAAEAFRPAESDGSSVDDSRTENGMMAETTTPENVFYMDEEAVFGMPG 180

Query: 181 LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212

BLAST of CmoCh16G001580 vs. ExPASy TrEMBL
Match: A0A5A7SWS3 (Dehydration-responsive element-binding protein 1A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G00440 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 188/212 (88.68%), Postives = 200/212 (94.34%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M+SFS FYE+YFSSSESS CRR IFS+++ MLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MDSFSNFYEEYFSSSESSNCRRPIFSDEDFMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAA+ LRG +ACLNFADSAWRLP PA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAIALRGLTACLNFADSAWRLPVPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAE-TTPENVLYVDDDAIFGLPG 180
           S DARDIQKAAAEAAEAFRPAESDGSSVDDSRTEN M AE TTPENV Y+D++A+FG+PG
Sbjct: 121 STDARDIQKAAAEAAEAFRPAESDGSSVDDSRTENGMMAETTTPENVFYMDEEAVFGMPG 180

Query: 181 LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LLEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212

BLAST of CmoCh16G001580 vs. NCBI nr
Match: XP_022922975.1 (dehydration-responsive element-binding protein 1A-like [Cucurbita moschata] >KAG7014791.1 Dehydration-responsive element-binding protein 1D, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 419.1 bits (1076), Expect = 2.3e-113
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180
           SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL
Sbjct: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180

Query: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 211

BLAST of CmoCh16G001580 vs. NCBI nr
Match: KAG6576749.1 (Dehydration-responsive element-binding protein 1D, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 417.2 bits (1071), Expect = 8.7e-113
Identity = 210/211 (99.53%), Postives = 210/211 (99.53%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           MESFSTFYEDYFSSSESSTCRR IFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MESFSTFYEDYFSSSESSTCRRAIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180
           SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL
Sbjct: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180

Query: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 211

BLAST of CmoCh16G001580 vs. NCBI nr
Match: XP_023522016.1 (dehydration-responsive element-binding protein 1A-like [Cucurbita pepo subsp. pepo] >XP_023553052.1 dehydration-responsive element-binding protein 1A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 209/211 (99.05%), Postives = 211/211 (100.00%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           MESFSTFYEDYFSSSESSTCRR+IFS+KELMLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MESFSTFYEDYFSSSESSTCRRSIFSDKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180
           SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL
Sbjct: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180

Query: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 211

BLAST of CmoCh16G001580 vs. NCBI nr
Match: XP_022984775.1 (dehydration-responsive element-binding protein 1A-like [Cucurbita maxima])

HSP 1 Score: 411.8 bits (1057), Expect = 3.7e-111
Identity = 208/211 (98.58%), Postives = 208/211 (98.58%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           MESFST YEDYFSSSESS CRR IFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MESFSTLYEDYFSSSESSDCRRFIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180
           SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL
Sbjct: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180

Query: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 211

BLAST of CmoCh16G001580 vs. NCBI nr
Match: XP_038900545.1 (dehydration-responsive element-binding protein 1A-like [Benincasa hispida])

HSP 1 Score: 379.4 bits (973), Expect = 2.0e-101
Identity = 187/211 (88.63%), Postives = 199/211 (94.31%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M+SFSTFYEDYFSSSESS CRR  + ++E+MLA SNPKKRAGRKKFKETRHPVYRGVRRR
Sbjct: 1   MDSFSTFYEDYFSSSESSNCRRPFYLDEEIMLAESNPKKRAGRKKFKETRHPVYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAA+ LRG +ACLNFADSAWRLP PA
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAALALRGLTACLNFADSAWRLPVPA 120

Query: 121 SADARDIQKAAAEAAEAFRPAESDGSSVDDSRTENMMTAETTPENVLYVDDDAIFGLPGL 180
           SADARDIQKAAAEAAEAFRP ESD SSVDDSRTEN MTAETTPENV Y+D++A+FG+PGL
Sbjct: 121 SADARDIQKAAAEAAEAFRPVESDRSSVDDSRTENWMTAETTPENVFYMDEEAVFGMPGL 180

Query: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 212
           LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY
Sbjct: 181 LEDMAAGMMLPPPQHFRDDMDFYSDVSLWSY 211

BLAST of CmoCh16G001580 vs. TAIR 10
Match: AT4G25480.1 (dehydration response element B1A )

HSP 1 Score: 221.9 bits (564), Expect = 5.0e-58
Identity = 133/222 (59.91%), Postives = 154/222 (69.37%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M SFS F E + S  ESS      +      LA+S PKK AGRKKF+ETRHP+YRGVRRR
Sbjct: 1   MNSFSAFSEMFGSDYESSVSSGGDYIP---TLASSCPKKPAGRKKFRETRHPIYRGVRRR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKK+RIWLGTF TAEMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVCEVREPNKKTRIWLGTFQTAEMAARAHDVAALALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFRPAESD-----GSSVDDSRTENMMTAETTPENVLYVDDDAIF 180
           S  A+DIQKAAAEAA AF+    D     G  ++++  E + TAE + EN  Y+ D+A+F
Sbjct: 121 STCAKDIQKAAAEAALAFQDEMCDATTDHGFDMEETLVEAIYTAEQS-ENAFYMHDEAMF 180

Query: 181 GLPGLLEDMAAGMMLPPP------QHFRDDMDFYSDVSLWSY 212
            +P LL +MA GM+LP P       H  D  D   DVSLWSY
Sbjct: 181 EMPSLLANMAEGMLLPLPSVQWNHNHEVDGDD--DDVSLWSY 216

BLAST of CmoCh16G001580 vs. TAIR 10
Match: AT5G51990.1 (C-repeat-binding factor 4 )

HSP 1 Score: 219.2 bits (557), Expect = 3.2e-57
Identity = 125/223 (56.05%), Postives = 152/223 (68.16%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M  F + + D F S        +  SE    LA+S PKKRAGRKKF+ETRHP+YRGVR+R
Sbjct: 1   MNPFYSTFPDSFLSISDHRSPVSDSSECSPKLASSCPKKRAGRKKFRETRHPIYRGVRQR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCEVREPNKKSRIWLGTFPT EMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVCEVREPNKKSRIWLGTFPTVEMAARAHDVAALALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFR----------PAESDGSSVDDSRTENMMTAETTPENVLYVD 180
           +   ++IQKAA+EAA AF+           AE++ ++ +  R E    AE     V Y+D
Sbjct: 121 TTCPKEIQKAASEAAMAFQNETTTEGSKTAAEAEEAAGEGVR-EGERRAEEQNGGVFYMD 180

Query: 181 DDAIFGLPGLLEDMAAGMMLPPPQ--HFRDDMDFYSDVSLWSY 212
           D+A+ G+P   E+MA GM+LPPP+     +D D   DVSLWS+
Sbjct: 181 DEALLGMPNFFENMAEGMLLPPPEVGWNHNDFDGVGDVSLWSF 222

BLAST of CmoCh16G001580 vs. TAIR 10
Match: AT4G25470.1 (C-repeat/DRE binding factor 2 )

HSP 1 Score: 216.1 bits (549), Expect = 2.7e-56
Identity = 123/220 (55.91%), Postives = 156/220 (70.91%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M SFS F E + S  ES       +S K   LA S PKK AGRKKF+ETRHP+YRGVR+R
Sbjct: 1   MNSFSAFSEMFGSDYESPVSSGGDYSPK---LATSCPKKPAGRKKFRETRHPIYRGVRQR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWVCE+REPNKK+RIWLGTF TAEMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVCELREPNKKTRIWLGTFQTAEMAARAHDVAAIALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFR------PAESDGSSVDDSRTENMMTAETTPENVLYVDDDAI 180
           S  A++IQKAAAEAA  F+        ++ G  ++++  E + T E + ++  Y+D++A+
Sbjct: 121 STCAKEIQKAAAEAALNFQDEMCHMTTDAHGLDMEETLVEAIYTPEQS-QDAFYMDEEAM 180

Query: 181 FGLPGLLEDMAAGMMLPPPQ---HFRDDMDFYSDVSLWSY 212
            G+  LL++MA GM+LP P    ++  D++   DVSLWSY
Sbjct: 181 LGMSSLLDNMAEGMLLPSPSVQWNYNFDVEGDDDVSLWSY 216

BLAST of CmoCh16G001580 vs. TAIR 10
Match: AT4G25490.1 (C-repeat/DRE binding factor 1 )

HSP 1 Score: 214.9 bits (546), Expect = 6.1e-56
Identity = 126/220 (57.27%), Postives = 151/220 (68.64%), Query Frame = 0

Query: 1   MESFSTFYEDYFSSSESSTCRRTIFSEKELMLAASNPKKRAGRKKFKETRHPVYRGVRRR 60
           M SFS F E + S  E          +    LA S PKK AGRKKF+ETRHP+YRGVR+R
Sbjct: 1   MNSFSAFSEMFGSDYEPQG------GDYCPTLATSCPKKPAGRKKFRETRHPIYRGVRQR 60

Query: 61  NSGKWVCEVREPNKKSRIWLGTFPTAEMAARAHDVAAMKLRGRSACLNFADSAWRLPEPA 120
           NSGKWV EVREPNKK+RIWLGTF TAEMAARAHDVAA+ LRGRSACLNFADSAWRL  P 
Sbjct: 61  NSGKWVSEVREPNKKTRIWLGTFQTAEMAARAHDVAALALRGRSACLNFADSAWRLRIPE 120

Query: 121 SADARDIQKAAAEAAEAFRPAESD------GSSVDDSRTENMMTAETTPENVLYVDDDAI 180
           S  A+DIQKAAAEAA AF+    D      G  ++++  E + T E + E   Y+D++ +
Sbjct: 121 STCAKDIQKAAAEAALAFQDETCDTTTTNHGLDMEETMVEAIYTPEQS-EGAFYMDEETM 180

Query: 181 FGLPGLLEDMAAGMMLPPPQ---HFRDDMDFYSDVSLWSY 212
           FG+P LL++MA GM+LPPP    +   D +   DVSLWSY
Sbjct: 181 FGMPTLLDNMAEGMLLPPPSVQWNHNYDGEGDGDVSLWSY 213

BLAST of CmoCh16G001580 vs. TAIR 10
Match: AT1G63030.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 177.9 bits (450), Expect = 8.3e-45
Identity = 100/192 (52.08%), Postives = 128/192 (66.67%), Query Frame = 0

Query: 29  ELMLAASNPKKRAGRKKFKETRHPVYRGVRRRNSGKWVCEVREPNKKSRIWLGTFPTAEM 88
           ++ +A   PKKRAGR+ FKETRHP+YRGVRRR+  KWVCEVREP  + R+WLGT+PTA+M
Sbjct: 5   DITVAEMKPKKRAGRRIFKETRHPIYRGVRRRDGDKWVCEVREPIHQRRVWLGTYPTADM 64

Query: 89  AARAHDVAAMKLRGRSACLNFADSAWRLPEPASADARDIQKAAAEAAEAFRPAE-SDGSS 148
           AARAHDVA + LRGRSACLNF+DSAWRLP PAS D   I++ AAEAAE FRP E S G +
Sbjct: 65  AARAHDVAVLALRGRSACLNFSDSAWRLPVPASTDPDTIRRTAAEAAEMFRPPEFSTGIT 124

Query: 149 VDDSRTENMMTAETTPENVLYVDDDAIFGLPGLLEDMAAGMMLPPPQH--------FRDD 208
           V  S +E     +T+ E           G+ G++  +A   ++ PP+         + D+
Sbjct: 125 VLPSASE----FDTSDE-----------GVAGMMMRLAEEPLMSPPRSYIDMNTSVYVDE 181

Query: 209 MDFYSDVSLWSY 212
              Y D+SLWSY
Sbjct: 185 EMCYEDLSLWSY 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0L07.0e-5759.91Dehydration-responsive element-binding protein 1A OS=Arabidopsis thaliana OX=370... [more]
Q9FJ934.6e-5656.05Dehydration-responsive element-binding protein 1D OS=Arabidopsis thaliana OX=370... [more]
Q9SYS63.9e-5555.91Dehydration-responsive element-binding protein 1C OS=Arabidopsis thaliana OX=370... [more]
P938358.6e-5557.27Dehydration-responsive element-binding protein 1B OS=Arabidopsis thaliana OX=370... [more]
Q9SGJ61.2e-4352.08Dehydration-responsive element-binding protein 1E OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1EAB51.1e-113100.00dehydration-responsive element-binding protein 1A-like OS=Cucurbita moschata OX=... [more]
A0A6J1J9I51.8e-11198.58dehydration-responsive element-binding protein 1A-like OS=Cucurbita maxima OX=36... [more]
A0A5D3DFF63.7e-10189.15Dehydration-responsive element-binding protein 1A OS=Cucumis melo var. makuwa OX... [more]
A0A140DCV61.1e-10088.68CRT/DRE-binding factor 1 OS=Cucumis melo OX=3656 GN=LOC103484041 PE=2 SV=1[more]
A0A5A7SWS31.1e-10088.68Dehydration-responsive element-binding protein 1A OS=Cucumis melo var. makuwa OX... [more]
Match NameE-valueIdentityDescription
XP_022922975.12.3e-113100.00dehydration-responsive element-binding protein 1A-like [Cucurbita moschata] >KAG... [more]
KAG6576749.18.7e-11399.53Dehydration-responsive element-binding protein 1D, partial [Cucurbita argyrosper... [more]
XP_023522016.11.5e-11299.05dehydration-responsive element-binding protein 1A-like [Cucurbita pepo subsp. pe... [more]
XP_022984775.13.7e-11198.58dehydration-responsive element-binding protein 1A-like [Cucurbita maxima][more]
XP_038900545.12.0e-10188.63dehydration-responsive element-binding protein 1A-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G25480.15.0e-5859.91dehydration response element B1A [more]
AT5G51990.13.2e-5756.05C-repeat-binding factor 4 [more]
AT4G25470.12.7e-5655.91C-repeat/DRE binding factor 2 [more]
AT4G25490.16.1e-5657.27C-repeat/DRE binding factor 1 [more]
AT1G63030.18.3e-4552.08Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 54..65
score: 66.06
coord: 76..92
score: 70.05
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 53..116
e-value: 3.2E-31
score: 119.7
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 52..102
e-value: 5.2E-16
score: 58.6
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 53..110
score: 23.077003
IPR001471AP2/ERF domainCDDcd00018AP2coord: 54..112
e-value: 5.84066E-32
score: 108.898
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 53..111
e-value: 2.6E-24
score: 87.1
NoneNo IPR availablePANTHERPTHR31839DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1Dcoord: 13..211
NoneNo IPR availablePANTHERPTHR31839:SF53AP2/ERF DOMAIN-CONTAINING TRANSCRIPTION FACTORcoord: 13..211
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 53..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G001580.1CmoCh16G001580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity