CmoCh16G001360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G001360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmethyl-CpG-binding domain-containing protein 13-like isoform X1
LocationCmo_Chr16: 633961 .. 637591 (+)
RNA-Seq ExpressionCmoCh16G001360
SyntenyCmoCh16G001360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGCAGCCCGCTCTTTTTTCGAATCTCATCTTAGAATTCGTACCTCCATTGTTGAGATTAACTGAAGCTTCGATATTCCCAAAAAACATATATGTTTGGTGCATTGATACTGCACCCTTTTACTCTCCCGTTGCAGTAGACGTTCCCGGAACCTCTTGCGGTTTCAATCCTCTGCTTCACTCGCAAAAAGTTATCGTAGTCGTCCACTTTGCACGGTGGTACATTTGAATCGAGCTGCGTTTTAAACTTGCAAATGGGTCCAATGTACCAATCGAAAGATCCAGAGAAGTGGGTTGTAGAAAATTTTGAAGGGGAGAGTTATAGAGGATATTACATGGGGGTCTGACACGTTTATATGCCTCGCGGGGGGTTTCTCAGAACACGTGGTTTGGAAGAATATTTTGTCGGAATCCTCCATTTTTTATATCCCCTTGAGGGAATTTGACTCTGAAATCAGGAACTTGGCGAGCCGGAAGAGAGAAAAGAGAAAGGGTTATTGAGATTGGCAACTGGGTTTCACCGGAAAGTCCGAGTATCTAGCTGAATGATGAACCAGAATTCAGAAAACTGGCTACCACCCGGCTGGACTGTGAAGGTCAAAGTGAGGAAGAGTGGCAAGAAGGATAAGGTACTGTTTTCATTTTCTTTCTCCTGTGATTTTTACTTGCATTTCATAGATCTTTATCTGACTTCGCGACTTTTATGTTCCACATTGCTGTTACAACTAGCTTTTGATTTCAACTTGTATAGATTCTTACTGATCTGAGTAAATGGCTTACCTTCTTCTTGCTCCTTTGGCTATGATGCTCCCATATAATCCTTGGCCAAGGTTCTATCTGCATTTTGAATCAACTAGTTCATCCCATTTTAGGCCCTTTCTGTTTAGTCTTGCGTGAAGCAGCAAACTATGGTATCTTTTTCCTTCAGAAGAGGGAACTACAGCTTGTAATATTAGTGCAACTTCCATTAATGACTTAACAACATAACATGCCTCTTCCTATTTTAATGTCTTCTAGTTATTTGCTTTTGGTATTCAAATATTTGTTTGATAACCATGAATTCTACTACCATTTGGATGTTCATATGAAAAGTAAGTCTGAATAAAGATCACAAAACTGATGGTAGCCAATTATGTTCTGTTTTTTTCATTTCAAGATAAGTAATTAGTTGGATCTTTGAGACTAATTCTAATGTTGCAGTATTATTTTGAACCCTCAAGTCAAATGAGATTTAATTCTAGGGCAGAGGTGTTTAGATACCTCAAAACTCCTGCAATCAATCATCCTGAATCTGAAGGGAGCAGAACCGTGGAGCAACCAGAGAACAATGTCTGTCAAGAAATCTACCCTCAACTCTTTCTTCTTCTTCTTCTTTTGTTAATCCCTTATTGATTTTTCTTTAAAAACTTGTATTATTCAAGCAATCTAGGTGGAAGTTAAGAAGACTATAGCAAAAGGGTTACCTCCTGGATGGATCAGAGAAATCAGAGTAACGAGGACTGCTAACAGAATAAGAAGAGATTCAGTAATTGATCATCTATTTCCTTTACGTAGTTTCGATTTGTCGTGATGTTTGGATTTTGGATATGGTCTCTAACTTTGCACTTTGTCTCATCCATATATTTAGTACCCAAAAGTTGGTGACATGCTAAACAGCATGAATATTAACAAATGAAACTTGTATTTGCAGTTTTACATTAATCCTGTAAATGGAAATGCGCTTCGCTCGTTGAGGGATGTACATCGGTATCTAACAAGTGGAAAGGTGAGCCGATTAGCTTATAAATCAAGGAGCCAGAGCAACAGCAACAGCAACAGCAACATTGAGTTTCAGCACGATGATATCTCTGTAAGTGTTCTAAATGGCTGAATTTTGGGTGAATTCGTCTCTTTTAAAATATGTACATAAATTTTGGCCTGTTATCATGGATCCCGAATCAATGAAACCCATTAATACTTTTCTAGCTACTTGGCAATCTGAAATCTCAGAGCTATGGCTGTTTGACACTACTGTAAGCTCTCTCAATCTTTCGAGGATTAGACCGACTTGAGATTTTTAAGGTTTCTGAAACAGTTTGGATATTTTCTTGCTTTTAAACCCAAAATGCAAAAAGAAATCTGTTTCAAACCGAAATTTGATTTCTCTTTTTCTCTTAGTCACCTTCTGTCTCCGAGAAAGAGATGCTAGCGATTGGCAAAGCAAAGAGACAGATTATATGGAGCGACAACTCACCGGAACCGAGTGAAATAGTAGATGATGAAGCTATTTTTCCAAAGGCTGCAAGTGTTGGGGAAAGAATGCCCTTTTTGAAACCTAATTTAACTCACGGTATTGTTCTGAACATGCAGCTTTAGTGTGACATTTCTCCATCACTTATAATATGTGCGTTAAGTAAGTAGGTAGAGTGGGAGTTCATGCATTTTGAACAATGAAGCTAAAGATTCCAGTTGCTGTTATGGAATGCTGTAAAAGTAGATTGATTGCTTTGAAAAATTTGTTTAACATAATATGTAGAACACATGTACAGTGCTTAGAAGGGTTTTCATTTTTGAAGATTCTGTATCTGATGTGAATTTAGAAACTACTGCAGGGAGGATAGGCGCTAAGTTGCACTGCTCAACTCCCGAAGAGGCAAAAGAACGTGAGCAATCAGAAGGAAAGAATAATTTTTCCGACTTTGTGCCAACTCCTGATAAATCCTTCCAACACAAGTGCACTAGTGAGAATGGGGCAACAAAGATTGAGAGTAGAAAGAGACAAAGGAAAAGTAAAGACATAAACTTGCGATGCCGTGCTTCAAAGCGACTTGCAGGGCTCCAAGCTGAGCCAGTGCTTGAAGCTAAAACTGGTCACCAAGCGTGTTCGATTGCATGTGAAGAGTCTGATAAGCAAGTAGCCAGTACAACTAAGTGGCCATCTCAAAGTCCTGAAAATCCTGATGTCAAGCATGAAACAAAGAGCATAATTGACCCTCCTAAGATTGACACAAATCTAGATTCAAGTAGAAAAGAACATTCTGCTGTTGACTTGTCCATTCTGCCTGAGAAGGTTAAGAAGATGAAATCTGAAGATGCATATGAGCTGCAGCAGCCGAAATGCAATTCCTTTCTGCCTTCAGAGGACGTTCTGGAAAAGCATGTAGGGCAGGTGGAAAGTGATGATAAGGCTGATGTGAAAAAACAAGGCACTCTTCTTAAACTGCCTATGGAGGACTTACTGACTGATCCTTGCATTGCATTTGCAGTTAAAACGCTTACTGGAGACGTTTTTGACGCATCCATAAGCTCAGAAGCATCACTGATGCCAAACAATATCGATCGGCCTTTCGTGGCCTTTACTCACTTGGAATCAGAAAACAAGCTTAATGAGAAGTTGCCATCTCCAGAACTTCCTGTTACTCGAGTTGGGAAAGTCGAAAATAACAACATTGTTGAAAAGCTAGACTCGACTCTGAAGCTGCCAGTTGGGGAAATATGGGCGGACCCTTGTATAGAATTTGCTATTAAAACTCTTACTGGTGAGATCCCACTTGTTAACAATCCAGATATTGAGGATTACTTTCACCAATTTGCCCCCTCAAAAACCGAAGGAACTAACGCAAATGCCTCGAATCATGTTGGTTCGTATTAG

mRNA sequence

CAAGCAGCCCGCTCTTTTTTCGAATCTCATCTTAGAATTCGTACCTCCATTGTTGAGATTAACTGAAGCTTCGATATTCCCAAAAAACATATATGTTTGGTGCATTGATACTGCACCCTTTTACTCTCCCGTTGCAGTAGACGTTCCCGGAACCTCTTGCGGTTTCAATCCTCTGCTTCACTCGCAAAAAGTTATCGTAGTCGTCCACTTTGCACGGTGGTACATTTGAATCGAGCTGCGTTTTAAACTTGCAAATGGGTCCAATGTACCAATCGAAAGATCCAGAGAAGTGGGTTGTAGAAAATTTTGAAGGGGAGAGTTATAGAGGATATTACATGGGGGTCTGACACGTTTATATGCCTCGCGGGGGGTTTCTCAGAACACGTGGTTTGGAAGAATATTTTGTCGGAATCCTCCATTTTTTATATCCCCTTGAGGGAATTTGACTCTGAAATCAGGAACTTGGCGAGCCGGAAGAGAGAAAAGAGAAAGGGTTATTGAGATTGGCAACTGGGTTTCACCGGAAAGTCCGAGTATCTAGCTGAATGATGAACCAGAATTCAGAAAACTGGCTACCACCCGGCTGGACTGTGAAGGTCAAAGTGAGGAAGAGTGGCAAGAAGGATAAGTATTATTTTGAACCCTCAAGTCAAATGAGATTTAATTCTAGGGCAGAGGTGTTTAGATACCTCAAAACTCCTGCAATCAATCATCCTGAATCTGAAGGGAGCAGAACCGTGGAGCAACCAGAGAACAATGTGGAAGTTAAGAAGACTATAGCAAAAGGGTTACCTCCTGGATGGATCAGAGAAATCAGAGTAACGAGGACTGCTAACAGAATAAGAAGAGATTCATTTTACATTAATCCTGTAAATGGAAATGCGCTTCGCTCGTTGAGGGATGTACATCGGTATCTAACAAGTGGAAAGGTGAGCCGATTAGCTTATAAATCAAGGAGCCAGAGCAACAGCAACAGCAACAGCAACATTGAGTTTCAGCACGATGATATCTCTTCACCTTCTGTCTCCGAGAAAGAGATGCTAGCGATTGGCAAAGCAAAGAGACAGATTATATGGAGCGACAACTCACCGGAACCGAGTGAAATAGTAGATGATGAAGCTATTTTTCCAAAGGCTGCAAGTGTTGGGGAAAGAATGCCCTTTTTGAAACCTAATTTAACTCACGGTATTAACACATGTACAGTGCTTAGAAGGGTTTTCATTTTTGAAGATTCTGTATCTGATGTGAATTTAGAAACTACTGCAGGGAGGATAGGCGCTAAGTTGCACTGCTCAACTCCCGAAGAGGCAAAAGAACGTGAGCAATCAGAAGGAAAGAATAATTTTTCCGACTTTGTGCCAACTCCTGATAAATCCTTCCAACACAAGTGCACTAGTGAGAATGGGGCAACAAAGATTGAGAGTAGAAAGAGACAAAGGAAAAGTAAAGACATAAACTTGCGATGCCGTGCTTCAAAGCGACTTGCAGGGCTCCAAGCTGAGCCAGTGCTTGAAGCTAAAACTGGTCACCAAGCGTGTTCGATTGCATGTGAAGAGTCTGATAAGCAAGTAGCCAGTACAACTAAGTGGCCATCTCAAAGTCCTGAAAATCCTGATGTCAAGCATGAAACAAAGAGCATAATTGACCCTCCTAAGATTGACACAAATCTAGATTCAAGTAGAAAAGAACATTCTGCTGTTGACTTGTCCATTCTGCCTGAGAAGGTTAAGAAGATGAAATCTGAAGATGCATATGAGCTGCAGCAGCCGAAATGCAATTCCTTTCTGCCTTCAGAGGACGTTCTGGAAAAGCATGTAGGGCAGGTGGAAAGTGATGATAAGGCTGATGTGAAAAAACAAGGCACTCTTCTTAAACTGCCTATGGAGGACTTACTGACTGATCCTTGCATTGCATTTGCAGTTAAAACGCTTACTGGAGACGTTTTTGACGCATCCATAAGCTCAGAAGCATCACTGATGCCAAACAATATCGATCGGCCTTTCGTGGCCTTTACTCACTTGGAATCAGAAAACAAGCTTAATGAGAAGTTGCCATCTCCAGAACTTCCTGTTACTCGAGTTGGGAAAGTCGAAAATAACAACATTGTTGAAAAGCTAGACTCGACTCTGAAGCTGCCAGTTGGGGAAATATGGGCGGACCCTTGTATAGAATTTGCTATTAAAACTCTTACTGGTGAGATCCCACTTGTTAACAATCCAGATATTGAGGATTACTTTCACCAATTTGCCCCCTCAAAAACCGAAGGAACTAACGCAAATGCCTCGAATCATGTTGGTTCGTATTAG

Coding sequence (CDS)

ATGATGAACCAGAATTCAGAAAACTGGCTACCACCCGGCTGGACTGTGAAGGTCAAAGTGAGGAAGAGTGGCAAGAAGGATAAGTATTATTTTGAACCCTCAAGTCAAATGAGATTTAATTCTAGGGCAGAGGTGTTTAGATACCTCAAAACTCCTGCAATCAATCATCCTGAATCTGAAGGGAGCAGAACCGTGGAGCAACCAGAGAACAATGTGGAAGTTAAGAAGACTATAGCAAAAGGGTTACCTCCTGGATGGATCAGAGAAATCAGAGTAACGAGGACTGCTAACAGAATAAGAAGAGATTCATTTTACATTAATCCTGTAAATGGAAATGCGCTTCGCTCGTTGAGGGATGTACATCGGTATCTAACAAGTGGAAAGGTGAGCCGATTAGCTTATAAATCAAGGAGCCAGAGCAACAGCAACAGCAACAGCAACATTGAGTTTCAGCACGATGATATCTCTTCACCTTCTGTCTCCGAGAAAGAGATGCTAGCGATTGGCAAAGCAAAGAGACAGATTATATGGAGCGACAACTCACCGGAACCGAGTGAAATAGTAGATGATGAAGCTATTTTTCCAAAGGCTGCAAGTGTTGGGGAAAGAATGCCCTTTTTGAAACCTAATTTAACTCACGGTATTAACACATGTACAGTGCTTAGAAGGGTTTTCATTTTTGAAGATTCTGTATCTGATGTGAATTTAGAAACTACTGCAGGGAGGATAGGCGCTAAGTTGCACTGCTCAACTCCCGAAGAGGCAAAAGAACGTGAGCAATCAGAAGGAAAGAATAATTTTTCCGACTTTGTGCCAACTCCTGATAAATCCTTCCAACACAAGTGCACTAGTGAGAATGGGGCAACAAAGATTGAGAGTAGAAAGAGACAAAGGAAAAGTAAAGACATAAACTTGCGATGCCGTGCTTCAAAGCGACTTGCAGGGCTCCAAGCTGAGCCAGTGCTTGAAGCTAAAACTGGTCACCAAGCGTGTTCGATTGCATGTGAAGAGTCTGATAAGCAAGTAGCCAGTACAACTAAGTGGCCATCTCAAAGTCCTGAAAATCCTGATGTCAAGCATGAAACAAAGAGCATAATTGACCCTCCTAAGATTGACACAAATCTAGATTCAAGTAGAAAAGAACATTCTGCTGTTGACTTGTCCATTCTGCCTGAGAAGGTTAAGAAGATGAAATCTGAAGATGCATATGAGCTGCAGCAGCCGAAATGCAATTCCTTTCTGCCTTCAGAGGACGTTCTGGAAAAGCATGTAGGGCAGGTGGAAAGTGATGATAAGGCTGATGTGAAAAAACAAGGCACTCTTCTTAAACTGCCTATGGAGGACTTACTGACTGATCCTTGCATTGCATTTGCAGTTAAAACGCTTACTGGAGACGTTTTTGACGCATCCATAAGCTCAGAAGCATCACTGATGCCAAACAATATCGATCGGCCTTTCGTGGCCTTTACTCACTTGGAATCAGAAAACAAGCTTAATGAGAAGTTGCCATCTCCAGAACTTCCTGTTACTCGAGTTGGGAAAGTCGAAAATAACAACATTGTTGAAAAGCTAGACTCGACTCTGAAGCTGCCAGTTGGGGAAATATGGGCGGACCCTTGTATAGAATTTGCTATTAAAACTCTTACTGGTGAGATCCCACTTGTTAACAATCCAGATATTGAGGATTACTTTCACCAATTTGCCCCCTCAAAAACCGAAGGAACTAACGCAAATGCCTCGAATCATGTTGGTTCGTATTAG

Protein sequence

MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIFEDSVSDVNLETTAGRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY
Homology
BLAST of CmoCh16G001360 vs. ExPASy Swiss-Prot
Match: Q9LTJ8 (Methyl-CpG-binding domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=MBD13 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.0e-09
Identity = 44/115 (38.26%), Postives = 58/115 (50.43%), Query Frame = 0

Query: 446 MEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSP 505
           + DL  DPCIAFA+KTLTG+                ++ P ++   + +  K      +P
Sbjct: 602 LADLCKDPCIAFAIKTLTGESLCL------------LNTPAISSNPINNHTKQKGVSFTP 661

Query: 506 ELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPL-VNNPD 560
           E P    G V  N   EKL      P   IW DPCI+FAIKTLTG IP+ ++ PD
Sbjct: 662 ETP----GNV--NTCSEKLVFPSPPPGANIWQDPCIDFAIKTLTGAIPIGLDEPD 698


HSP 2 Score: 58.2 bits (139), Expect = 3.7e-07
Identity = 88/392 (22.45%), Postives = 161/392 (41.07%), Query Frame = 0

Query: 69  ENNVEVKKTIAKGLPPGWIREIRVT-RTANRIRRDSFYINPVNGNALRSLRDVHRYLTSG 128
           ++ V V+K+ A+GLP GWI+++ +T R+  + RRD F+I+P +    +S +D  RY+ +G
Sbjct: 26  KDKVIVEKSAAQGLPEGWIKKLEITNRSGRKTRRDPFFIDPKSEYIFQSFKDASRYVETG 85

Query: 129 KVSRLAYKSR-----SQSNSNSNSNIEFQH-DDISSPSVSEKEMLA--IGKAKRQII--- 188
            +   A K +        + N  + +  ++ D  S+  V EKE     + ++KR+ +   
Sbjct: 86  NIGHYARKLKESDIEDDDSGNGKTVLRLEYVDKRSADDVLEKEKTIDDVRRSKRRNLSSS 145

Query: 189 ---------WSDNSPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIF 248
                     SD S   S++++D     +     E+    K          T    V   
Sbjct: 146 DEHSKNCKMTSDLSIVTSQVLEDLGKKEEVKDPIEKQLIAKRVTRSQTKASTTEEVVVDL 205

Query: 249 EDSVSDVNLETTAGRIGAKLHCSTP-EEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSEN 308
           + ++S  N ++    + + +    P +EA  +E+ E                Q       
Sbjct: 206 KRNLSSSNAKSEKDSVNSSVRSQKPKKEAVMKEEEE----------------QDSSEKRI 265

Query: 309 GATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTGHQACSIA--CEESDKQVAS 368
             +K+E +K +  +   ++  R SKRLAG++ EP  E KT  +   I    +E   ++ +
Sbjct: 266 TRSKVEEKKNELSN---SVARRTSKRLAGIELEPTPELKTRAKVQRIVPLDDEPTPELKT 325

Query: 369 TTK----WPSQSPENPDVKHETK-SIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKS 428
            TK     P      P++K  TK   I PP  +  L+   +      L    E   ++KS
Sbjct: 326 RTKVQRVVPPDDEPTPELKTRTKIQRIVPPDDEPTLELKTRTKVQRILPPDDELTPELKS 385

Query: 429 EDAYELQQPKCNSFLPSEDVLEKHVGQVESDD 432
               +   P  +   P      K   ++  DD
Sbjct: 386 RTKVQRIVPPDDELTPEFKTRTKVQQRIPPDD 398

BLAST of CmoCh16G001360 vs. ExPASy Swiss-Prot
Match: Q9LTJ1 (Methyl-CpG-binding domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=MBD6 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.9e-07
Identity = 39/130 (30.00%), Postives = 53/130 (40.77%), Query Frame = 0

Query: 7   ENWLPPGWTVKVKVRKS----GKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGS 66
           +NWLPPGW V+ K+R S    G  DKYY+EP++  +F SR EV  YL+         +  
Sbjct: 78  DNWLPPGWRVEDKIRTSGATAGSVDKYYYEPNTGRKFRSRTEVLYYLEHGTSKRGTKKAE 137

Query: 67  RTVEQPE-------NNVEVKKTIAKGLPP-----------------------GWIREIRV 103
            T   P+       N V    T+    PP                       GWI  I  
Sbjct: 138 NTYFNPDHFEGQGSNRVTRTATVPPPPPPPLDFDFKNPPDKVSWSMANAGEEGWIPNIGD 197

BLAST of CmoCh16G001360 vs. ExPASy Swiss-Prot
Match: Q9SNC0 (Methyl-CpG-binding domain-containing protein 5 OS=Arabidopsis thaliana OX=3702 GN=MBD5 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.7e-05
Identity = 26/71 (36.62%), Postives = 42/71 (59.15%), Query Frame = 0

Query: 7  ENWLPPGWTVKVKVRKSGKK----DKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGS 66
          +NWLPP W  +++VR SG K    DK+Y+EP +  +F S+ EV  YL+      P+ +  
Sbjct: 32 DNWLPPDWRTEIRVRTSGTKAGTVDKFYYEPITGRKFRSKNEVLYYLEH---GTPKKKSV 91

Query: 67 RTVEQPENNVE 74
          +T E  +++ E
Sbjct: 92 KTAENGDSHSE 99

BLAST of CmoCh16G001360 vs. ExPASy TrEMBL
Match: A0A6J1EAU0 (methyl-CpG-binding domain-containing protein 13-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430888 PE=4 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 559/586 (95.39%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE 60
           MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE
Sbjct: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE 60

Query: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120
           GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV
Sbjct: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120

Query: 121 HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180
           HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN
Sbjct: 121 HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180

Query: 181 SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIFEDSVSDVNLETTA 240
           SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTH                           
Sbjct: 181 SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTH--------------------------- 240

Query: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS 300
           GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS
Sbjct: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS 300

Query: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360
           KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH
Sbjct: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360

Query: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL 420
           ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL
Sbjct: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL 420

Query: 421 EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN 480
           EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN
Sbjct: 421 EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN 480

Query: 481 NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC 540
           NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC
Sbjct: 481 NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC 540

Query: 541 IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 587
           IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY
Sbjct: 541 IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 559

BLAST of CmoCh16G001360 vs. ExPASy TrEMBL
Match: A0A6J1E5Z5 (methyl-CpG-binding domain-containing protein 13-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430888 PE=4 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 1.0e-297
Identity = 531/559 (94.99%), Postives = 532/559 (95.17%), Query Frame = 0

Query: 28  KYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWI 87
           +YYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWI
Sbjct: 3   QYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWI 62

Query: 88  REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSN 147
           REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSN
Sbjct: 63  REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSN 122

Query: 148 IEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFL 207
           IEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFL
Sbjct: 123 IEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFL 182

Query: 208 KPNLTHGINTCTVLRRVFIFEDSVSDVNLETTAGRIGAKLHCSTPEEAKEREQSEGKNNF 267
           KPNLTH                           GRIGAKLHCSTPEEAKEREQSEGKNNF
Sbjct: 183 KPNLTH---------------------------GRIGAKLHCSTPEEAKEREQSEGKNNF 242

Query: 268 SDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTG 327
           SDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTG
Sbjct: 243 SDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTG 302

Query: 328 HQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDL 387
           HQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDL
Sbjct: 303 HQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDL 362

Query: 388 SILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPME 447
           SILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPME
Sbjct: 363 SILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPME 422

Query: 448 DLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPEL 507
           DLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPEL
Sbjct: 423 DLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPEL 482

Query: 508 PVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQF 567
           PVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQF
Sbjct: 483 PVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQF 534

Query: 568 APSKTEGTNANASNHVGSY 587
           APSKTEGTNANASNHVGSY
Sbjct: 543 APSKTEGTNANASNHVGSY 534

BLAST of CmoCh16G001360 vs. ExPASy TrEMBL
Match: A0A6J1E5B7 (methyl-CpG-binding domain-containing protein 13-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430888 PE=4 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.7e-292
Identity = 523/550 (95.09%), Postives = 523/550 (95.09%), Query Frame = 0

Query: 37  MRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTA 96
           MRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTA
Sbjct: 1   MRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTA 60

Query: 97  NRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDIS 156
           NRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDIS
Sbjct: 61  NRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDIS 120

Query: 157 SPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGIN 216
           SPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFLKPNLTH   
Sbjct: 121 SPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFLKPNLTH--- 180

Query: 217 TCTVLRRVFIFEDSVSDVNLETTAGRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDK 276
                                   GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDK
Sbjct: 181 ------------------------GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDK 240

Query: 277 SFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACE 336
           SFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACE
Sbjct: 241 SFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACE 300

Query: 337 ESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKK 396
           ESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKK
Sbjct: 301 ESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKK 360

Query: 397 MKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIA 456
           MKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIA
Sbjct: 361 MKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIA 420

Query: 457 FAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVE 516
           FAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVE
Sbjct: 421 FAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVE 480

Query: 517 NNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTN 576
           NNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTN
Sbjct: 481 NNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTN 523

Query: 577 ANASNHVGSY 587
           ANASNHVGSY
Sbjct: 541 ANASNHVGSY 523

BLAST of CmoCh16G001360 vs. ExPASy TrEMBL
Match: A0A6J1J2L9 (methyl-CpG-binding domain-containing protein 13-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482847 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.7e-289
Identity = 526/594 (88.55%), Postives = 540/594 (90.91%), Query Frame = 0

Query: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE 60
           MMNQNSENWLP GWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKT AINHPESE
Sbjct: 1   MMNQNSENWLPLGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTAAINHPESE 60

Query: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120
            SRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV
Sbjct: 61  ESRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120

Query: 121 HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180
           HRYLTSGKVSRLAYKSRSQ  SNSNSNIEFQHDDISS SVS+KEMLAIGKAKRQIIWSDN
Sbjct: 121 HRYLTSGKVSRLAYKSRSQ--SNSNSNIEFQHDDISSSSVSKKEMLAIGKAKRQIIWSDN 180

Query: 181 SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIFEDSVSDVNLETTA 240
           SPEPSEI DDEAIFPKAASVGERMPFLKPNLTH                           
Sbjct: 181 SPEPSEIADDEAIFPKAASVGERMPFLKPNLTH--------------------------- 240

Query: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS 300
           GRIGAKL CSTPEEAKEREQSEGKN+FS+FV TPDKS +HKCTSENGATKIESRKRQRKS
Sbjct: 241 GRIGAKLRCSTPEEAKEREQSEGKNSFSEFVSTPDKSIRHKCTSENGATKIESRKRQRKS 300

Query: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360
           KDINL CRASKRLAGLQAEPVLEAKT  +ACS+ACEESDKQVASTTKWPSQ PENPDVKH
Sbjct: 301 KDINLPCRASKRLAGLQAEPVLEAKTDRRACSVACEESDKQVASTTKWPSQCPENPDVKH 360

Query: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL 420
           ET+SIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYE QQPKCNSFLPSEDVL
Sbjct: 361 ETRSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYEQQQPKCNSFLPSEDVL 420

Query: 421 EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASIS---SEASL 480
           EKHVGQVES+D+A VKKQG LLKLPMEDLLTDPCIAFAVKTLTGDVFDASIS   SEASL
Sbjct: 421 EKHVGQVESEDEAGVKKQGPLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASEASL 480

Query: 481 MPNNIDRPFVAFT-----HLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPV 540
           MPNNIDRPFVAFT     +L SENKLNEK PSPELPVTRVGKVENNNIVEKL+STL+LPV
Sbjct: 481 MPNNIDRPFVAFTPSERRNLGSENKLNEKSPSPELPVTRVGKVENNNIVEKLESTLELPV 540

Query: 541 GEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 587
           GE+WADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKT+GTNANASNHVGSY
Sbjct: 541 GEMWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTKGTNANASNHVGSY 565

BLAST of CmoCh16G001360 vs. ExPASy TrEMBL
Match: A0A6J1JB16 (methyl-CpG-binding domain-containing protein 13-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482847 PE=4 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 8.5e-273
Identity = 499/567 (88.01%), Postives = 514/567 (90.65%), Query Frame = 0

Query: 28  KYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWI 87
           +YYFEPSSQMRFNSRAEVFRYLKT AINHPESE SRTVEQPENNVEVKKTIAKGLPPGWI
Sbjct: 3   QYYFEPSSQMRFNSRAEVFRYLKTAAINHPESEESRTVEQPENNVEVKKTIAKGLPPGWI 62

Query: 88  REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSN 147
           REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQ  SNSNSN
Sbjct: 63  REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQ--SNSNSN 122

Query: 148 IEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFL 207
           IEFQHDDISS SVS+KEMLAIGKAKRQIIWSDNSPEPSEI DDEAIFPKAASVGERMPFL
Sbjct: 123 IEFQHDDISSSSVSKKEMLAIGKAKRQIIWSDNSPEPSEIADDEAIFPKAASVGERMPFL 182

Query: 208 KPNLTHGINTCTVLRRVFIFEDSVSDVNLETTAGRIGAKLHCSTPEEAKEREQSEGKNNF 267
           KPNLTH                           GRIGAKL CSTPEEAKEREQSEGKN+F
Sbjct: 183 KPNLTH---------------------------GRIGAKLRCSTPEEAKEREQSEGKNSF 242

Query: 268 SDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTG 327
           S+FV TPDKS +HKCTSENGATKIESRKRQRKSKDINL CRASKRLAGLQAEPVLEAKT 
Sbjct: 243 SEFVSTPDKSIRHKCTSENGATKIESRKRQRKSKDINLPCRASKRLAGLQAEPVLEAKTD 302

Query: 328 HQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDL 387
            +ACS+ACEESDKQVASTTKWPSQ PENPDVKHET+SIIDPPKIDTNLDSSRKEHSAVDL
Sbjct: 303 RRACSVACEESDKQVASTTKWPSQCPENPDVKHETRSIIDPPKIDTNLDSSRKEHSAVDL 362

Query: 388 SILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPME 447
           SILPEKVKKMKSEDAYE QQPKCNSFLPSEDVLEKHVGQVES+D+A VKKQG LLKLPME
Sbjct: 363 SILPEKVKKMKSEDAYEQQQPKCNSFLPSEDVLEKHVGQVESEDEAGVKKQGPLLKLPME 422

Query: 448 DLLTDPCIAFAVKTLTGDVFDASIS---SEASLMPNNIDRPFVAFT-----HLESENKLN 507
           DLLTDPCIAFAVKTLTGDVFDASIS   SEASLMPNNIDRPFVAFT     +L SENKLN
Sbjct: 423 DLLTDPCIAFAVKTLTGDVFDASISSEASEASLMPNNIDRPFVAFTPSERRNLGSENKLN 482

Query: 508 EKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPD 567
           EK PSPELPVTRVGKVENNNIVEKL+STL+LPVGE+WADPCIEFAIKTLTGEIPLVNNPD
Sbjct: 483 EKSPSPELPVTRVGKVENNNIVEKLESTLELPVGEMWADPCIEFAIKTLTGEIPLVNNPD 540

Query: 568 IEDYFHQFAPSKTEGTNANASNHVGSY 587
           IEDYFHQFAPSKT+GTNANASNHVGSY
Sbjct: 543 IEDYFHQFAPSKTKGTNANASNHVGSY 540

BLAST of CmoCh16G001360 vs. NCBI nr
Match: XP_022923130.1 (methyl-CpG-binding domain-containing protein 13-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 559/586 (95.39%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE 60
           MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE
Sbjct: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE 60

Query: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120
           GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV
Sbjct: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120

Query: 121 HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180
           HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN
Sbjct: 121 HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180

Query: 181 SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIFEDSVSDVNLETTA 240
           SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTH                           
Sbjct: 181 SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTH--------------------------- 240

Query: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS 300
           GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS
Sbjct: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS 300

Query: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360
           KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH
Sbjct: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360

Query: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL 420
           ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL
Sbjct: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL 420

Query: 421 EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN 480
           EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN
Sbjct: 421 EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN 480

Query: 481 NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC 540
           NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC
Sbjct: 481 NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC 540

Query: 541 IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 587
           IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY
Sbjct: 541 IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 559

BLAST of CmoCh16G001360 vs. NCBI nr
Match: KAG7014774.1 (DNA mismatch repair protein MSH3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 558/580 (96.21%), Postives = 559/580 (96.38%), Query Frame = 0

Query: 4    QNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSR 63
            +NSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKT AINHPESEGSR
Sbjct: 1693 RNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTAAINHPESEGSR 1752

Query: 64   TVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRY 123
            TVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRY
Sbjct: 1753 TVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRY 1812

Query: 124  LTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPE 183
            LTSGKVSRLAYKSRSQ  SNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPE
Sbjct: 1813 LTSGKVSRLAYKSRSQ--SNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPE 1872

Query: 184  PSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIFEDSVSDVNLETTAGRI 243
            PSEI DDEAIFPKAAS                NTCTVLRRVFIFEDSVSDVNLETTAGRI
Sbjct: 1873 PSEIADDEAIFPKAAS----------------NTCTVLRRVFIFEDSVSDVNLETTAGRI 1932

Query: 244  GAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDI 303
            GAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDI
Sbjct: 1933 GAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDI 1992

Query: 304  NLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKHETK 363
            NLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKHETK
Sbjct: 1993 NLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKHETK 2052

Query: 364  SIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKH 423
            SIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKH
Sbjct: 2053 SIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKH 2112

Query: 424  VGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNID 483
            VGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNID
Sbjct: 2113 VGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNID 2172

Query: 484  RPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEF 543
            RPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEF
Sbjct: 2173 RPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEF 2232

Query: 544  AIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHV 584
            AIKTLT EIPLVNNPDIEDYFHQFAPSKTEGTNANASNHV
Sbjct: 2233 AIKTLTDEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHV 2254

BLAST of CmoCh16G001360 vs. NCBI nr
Match: KAG6576730.1 (Methyl-CpG-binding domain-containing protein 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.8e-309
Identity = 552/586 (94.20%), Postives = 552/586 (94.20%), Query Frame = 0

Query: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE 60
           MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKT AINHPESE
Sbjct: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTAAINHPESE 60

Query: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120
           GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV
Sbjct: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120

Query: 121 HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180
            RYLTSGKVSRLAYKSRSQ  SNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN
Sbjct: 121 RRYLTSGKVSRLAYKSRSQ--SNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180

Query: 181 SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIFEDSVSDVNLETTA 240
           SPEPSEI DDEAIFPKAASVGERMPFLKPNLTH                           
Sbjct: 181 SPEPSEIADDEAIFPKAASVGERMPFLKPNLTH--------------------------- 240

Query: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS 300
           GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPD SFQHKCTSENGATKIESRKRQRKS
Sbjct: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDISFQHKCTSENGATKIESRKRQRKS 300

Query: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360
           KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH
Sbjct: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360

Query: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYELQQPKCNSFLPSEDVL 420
           ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYE QQPKCNSFLPSEDVL
Sbjct: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYEQQQPKCNSFLPSEDVL 420

Query: 421 EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN 480
           EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN
Sbjct: 421 EKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPN 480

Query: 481 NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC 540
           NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC
Sbjct: 481 NIDRPFVAFTHLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPC 540

Query: 541 IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 587
           IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY
Sbjct: 541 IEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 557

BLAST of CmoCh16G001360 vs. NCBI nr
Match: XP_022923131.1 (methyl-CpG-binding domain-containing protein 13-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.1e-297
Identity = 531/559 (94.99%), Postives = 532/559 (95.17%), Query Frame = 0

Query: 28  KYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWI 87
           +YYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWI
Sbjct: 3   QYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGSRTVEQPENNVEVKKTIAKGLPPGWI 62

Query: 88  REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSN 147
           REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSN
Sbjct: 63  REIRVTRTANRIRRDSFYINPVNGNALRSLRDVHRYLTSGKVSRLAYKSRSQSNSNSNSN 122

Query: 148 IEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFL 207
           IEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFL
Sbjct: 123 IEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDNSPEPSEIVDDEAIFPKAASVGERMPFL 182

Query: 208 KPNLTHGINTCTVLRRVFIFEDSVSDVNLETTAGRIGAKLHCSTPEEAKEREQSEGKNNF 267
           KPNLTH                           GRIGAKLHCSTPEEAKEREQSEGKNNF
Sbjct: 183 KPNLTH---------------------------GRIGAKLHCSTPEEAKEREQSEGKNNF 242

Query: 268 SDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTG 327
           SDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTG
Sbjct: 243 SDFVPTPDKSFQHKCTSENGATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTG 302

Query: 328 HQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDL 387
           HQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDL
Sbjct: 303 HQACSIACEESDKQVASTTKWPSQSPENPDVKHETKSIIDPPKIDTNLDSSRKEHSAVDL 362

Query: 388 SILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPME 447
           SILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPME
Sbjct: 363 SILPEKVKKMKSEDAYELQQPKCNSFLPSEDVLEKHVGQVESDDKADVKKQGTLLKLPME 422

Query: 448 DLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPEL 507
           DLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPEL
Sbjct: 423 DLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSPEL 482

Query: 508 PVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQF 567
           PVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQF
Sbjct: 483 PVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQF 534

Query: 568 APSKTEGTNANASNHVGSY 587
           APSKTEGTNANASNHVGSY
Sbjct: 543 APSKTEGTNANASNHVGSY 534

BLAST of CmoCh16G001360 vs. NCBI nr
Match: XP_023552462.1 (methyl-CpG-binding domain-containing protein 13-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1022.7 bits (2643), Expect = 1.3e-294
Identity = 534/592 (90.20%), Postives = 543/592 (91.72%), Query Frame = 0

Query: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESE 60
           MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKT AI+HPESE
Sbjct: 1   MMNQNSENWLPPGWTVKVKVRKSGKKDKYYFEPSSQMRFNSRAEVFRYLKTAAISHPESE 60

Query: 61  GSRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120
            SRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV
Sbjct: 61  ESRTVEQPENNVEVKKTIAKGLPPGWIREIRVTRTANRIRRDSFYINPVNGNALRSLRDV 120

Query: 121 HRYLTSGKVSRLAYKSRSQSNSNSNSNIEFQHDDISSPSVSEKEMLAIGKAKRQIIWSDN 180
           HRYLTSGKVSRLAYKSRSQ  SNSNSNIEFQHDDISSP+VS+KEMLAIGKAKRQIIWSDN
Sbjct: 121 HRYLTSGKVSRLAYKSRSQ--SNSNSNIEFQHDDISSPAVSKKEMLAIGKAKRQIIWSDN 180

Query: 181 SPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIFEDSVSDVNLETTA 240
           SPEPSEI DDEAIFPKAASVGERM FLKPNLTH                           
Sbjct: 181 SPEPSEIADDEAIFPKAASVGERMAFLKPNLTH--------------------------- 240

Query: 241 GRIGAKLHCSTPEEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSENGATKIESRKRQRKS 300
           GRIGAKLHC+TPEEAK REQSEGKNNFS+FV TPDKS QHKCTSENGATKIESRKRQRKS
Sbjct: 241 GRIGAKLHCTTPEEAKGREQSEGKNNFSEFVSTPDKSIQHKCTSENGATKIESRKRQRKS 300

Query: 301 KDINLRCRASKRLAGLQAEPVLEAKTGHQACSIACEESDKQVASTTKWPSQSPENPDVKH 360
           KDINLRCRASKRLAGLQAEPVLEAKT  +ACS+ACEESDKQVASTTKWPSQ PE PDVKH
Sbjct: 301 KDINLRCRASKRLAGLQAEPVLEAKTDRRACSVACEESDKQVASTTKWPSQCPEKPDVKH 360

Query: 361 ETKSIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKSEDAYE-LQQPKCNSFLPSEDV 420
           ETKSIID PKIDTNLDSSRKEHSAVDLSILP+KVKKMKSEDAYE  QQPKCNSFLPSEDV
Sbjct: 361 ETKSIIDSPKIDTNLDSSRKEHSAVDLSILPQKVKKMKSEDAYEQQQQPKCNSFLPSEDV 420

Query: 421 LEKHVGQVESDDKADVKKQGTLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMP 480
           LEKHVGQVESDDKADVKKQG LLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMP
Sbjct: 421 LEKHVGQVESDDKADVKKQGPLLKLPMEDLLTDPCIAFAVKTLTGDVFDASISSEASLMP 480

Query: 481 NNIDRPFVAFT-----HLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGE 540
           NNIDRPFVAFT     +LESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGE
Sbjct: 481 NNIDRPFVAFTPNERRNLESENKLNEKLPSPELPVTRVGKVENNNIVEKLDSTLKLPVGE 540

Query: 541 IWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 587
           IWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY
Sbjct: 541 IWADPCIEFAIKTLTGEIPLVNNPDIEDYFHQFAPSKTEGTNANASNHVGSY 563

BLAST of CmoCh16G001360 vs. TAIR 10
Match: AT5G52230.1 (methyl-CPG-binding domain protein 13 )

HSP 1 Score: 66.6 bits (161), Expect = 7.5e-11
Identity = 44/115 (38.26%), Postives = 58/115 (50.43%), Query Frame = 0

Query: 446 MEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSP 505
           + DL  DPCIAFA+KTLTG+                ++ P ++   + +  K      +P
Sbjct: 602 LADLCKDPCIAFAIKTLTGESLCL------------LNTPAISSNPINNHTKQKGVSFTP 661

Query: 506 ELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPL-VNNPD 560
           E P    G V  N   EKL      P   IW DPCI+FAIKTLTG IP+ ++ PD
Sbjct: 662 ETP----GNV--NTCSEKLVFPSPPPGANIWQDPCIDFAIKTLTGAIPIGLDEPD 698


HSP 2 Score: 58.2 bits (139), Expect = 2.7e-08
Identity = 88/392 (22.45%), Postives = 161/392 (41.07%), Query Frame = 0

Query: 69  ENNVEVKKTIAKGLPPGWIREIRVT-RTANRIRRDSFYINPVNGNALRSLRDVHRYLTSG 128
           ++ V V+K+ A+GLP GWI+++ +T R+  + RRD F+I+P +    +S +D  RY+ +G
Sbjct: 26  KDKVIVEKSAAQGLPEGWIKKLEITNRSGRKTRRDPFFIDPKSEYIFQSFKDASRYVETG 85

Query: 129 KVSRLAYKSR-----SQSNSNSNSNIEFQH-DDISSPSVSEKEMLA--IGKAKRQII--- 188
            +   A K +        + N  + +  ++ D  S+  V EKE     + ++KR+ +   
Sbjct: 86  NIGHYARKLKESDIEDDDSGNGKTVLRLEYVDKRSADDVLEKEKTIDDVRRSKRRNLSSS 145

Query: 189 ---------WSDNSPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIF 248
                     SD S   S++++D     +     E+    K          T    V   
Sbjct: 146 DEHSKNCKMTSDLSIVTSQVLEDLGKKEEVKDPIEKQLIAKRVTRSQTKASTTEEVVVDL 205

Query: 249 EDSVSDVNLETTAGRIGAKLHCSTP-EEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSEN 308
           + ++S  N ++    + + +    P +EA  +E+ E                Q       
Sbjct: 206 KRNLSSSNAKSEKDSVNSSVRSQKPKKEAVMKEEEE----------------QDSSEKRI 265

Query: 309 GATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTGHQACSIA--CEESDKQVAS 368
             +K+E +K +  +   ++  R SKRLAG++ EP  E KT  +   I    +E   ++ +
Sbjct: 266 TRSKVEEKKNELSN---SVARRTSKRLAGIELEPTPELKTRAKVQRIVPLDDEPTPELKT 325

Query: 369 TTK----WPSQSPENPDVKHETK-SIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKS 428
            TK     P      P++K  TK   I PP  +  L+   +      L    E   ++KS
Sbjct: 326 RTKVQRVVPPDDEPTPELKTRTKIQRIVPPDDEPTLELKTRTKVQRILPPDDELTPELKS 385

Query: 429 EDAYELQQPKCNSFLPSEDVLEKHVGQVESDD 432
               +   P  +   P      K   ++  DD
Sbjct: 386 RTKVQRIVPPDDELTPEFKTRTKVQQRIPPDD 398

BLAST of CmoCh16G001360 vs. TAIR 10
Match: AT5G52230.2 (methyl-CPG-binding domain protein 13 )

HSP 1 Score: 66.6 bits (161), Expect = 7.5e-11
Identity = 44/115 (38.26%), Postives = 58/115 (50.43%), Query Frame = 0

Query: 446 MEDLLTDPCIAFAVKTLTGDVFDASISSEASLMPNNIDRPFVAFTHLESENKLNEKLPSP 505
           + DL  DPCIAFA+KTLTG+                ++ P ++   + +  K      +P
Sbjct: 602 LADLCKDPCIAFAIKTLTGESLCL------------LNTPAISSNPINNHTKQKGVSFTP 661

Query: 506 ELPVTRVGKVENNNIVEKLDSTLKLPVGEIWADPCIEFAIKTLTGEIPL-VNNPD 560
           E P    G V  N   EKL      P   IW DPCI+FAIKTLTG IP+ ++ PD
Sbjct: 662 ETP----GNV--NTCSEKLVFPSPPPGANIWQDPCIDFAIKTLTGAIPIGLDEPD 698


HSP 2 Score: 58.2 bits (139), Expect = 2.7e-08
Identity = 88/392 (22.45%), Postives = 161/392 (41.07%), Query Frame = 0

Query: 69  ENNVEVKKTIAKGLPPGWIREIRVT-RTANRIRRDSFYINPVNGNALRSLRDVHRYLTSG 128
           ++ V V+K+ A+GLP GWI+++ +T R+  + RRD F+I+P +    +S +D  RY+ +G
Sbjct: 26  KDKVIVEKSAAQGLPEGWIKKLEITNRSGRKTRRDPFFIDPKSEYIFQSFKDASRYVETG 85

Query: 129 KVSRLAYKSR-----SQSNSNSNSNIEFQH-DDISSPSVSEKEMLA--IGKAKRQII--- 188
            +   A K +        + N  + +  ++ D  S+  V EKE     + ++KR+ +   
Sbjct: 86  NIGHYARKLKESDIEDDDSGNGKTVLRLEYVDKRSADDVLEKEKTIDDVRRSKRRNLSSS 145

Query: 189 ---------WSDNSPEPSEIVDDEAIFPKAASVGERMPFLKPNLTHGINTCTVLRRVFIF 248
                     SD S   S++++D     +     E+    K          T    V   
Sbjct: 146 DEHSKNCKMTSDLSIVTSQVLEDLGKKEEVKDPIEKQLIAKRVTRSQTKASTTEEVVVDL 205

Query: 249 EDSVSDVNLETTAGRIGAKLHCSTP-EEAKEREQSEGKNNFSDFVPTPDKSFQHKCTSEN 308
           + ++S  N ++    + + +    P +EA  +E+ E                Q       
Sbjct: 206 KRNLSSSNAKSEKDSVNSSVRSQKPKKEAVMKEEEE----------------QDSSEKRI 265

Query: 309 GATKIESRKRQRKSKDINLRCRASKRLAGLQAEPVLEAKTGHQACSIA--CEESDKQVAS 368
             +K+E +K +  +   ++  R SKRLAG++ EP  E KT  +   I    +E   ++ +
Sbjct: 266 TRSKVEEKKNELSN---SVARRTSKRLAGIELEPTPELKTRAKVQRIVPLDDEPTPELKT 325

Query: 369 TTK----WPSQSPENPDVKHETK-SIIDPPKIDTNLDSSRKEHSAVDLSILPEKVKKMKS 428
            TK     P      P++K  TK   I PP  +  L+   +      L    E   ++KS
Sbjct: 326 RTKVQRVVPPDDEPTPELKTRTKIQRIVPPDDEPTLELKTRTKVQRILPPDDELTPELKS 385

Query: 429 EDAYELQQPKCNSFLPSEDVLEKHVGQVESDD 432
               +   P  +   P      K   ++  DD
Sbjct: 386 RTKVQRIVPPDDELTPEFKTRTKVQQRIPPDD 398

BLAST of CmoCh16G001360 vs. TAIR 10
Match: AT5G59380.1 (methyl-CPG-binding domain 6 )

HSP 1 Score: 57.8 bits (138), Expect = 3.5e-08
Identity = 39/130 (30.00%), Postives = 53/130 (40.77%), Query Frame = 0

Query: 7   ENWLPPGWTVKVKVRKS----GKKDKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGS 66
           +NWLPPGW V+ K+R S    G  DKYY+EP++  +F SR EV  YL+         +  
Sbjct: 78  DNWLPPGWRVEDKIRTSGATAGSVDKYYYEPNTGRKFRSRTEVLYYLEHGTSKRGTKKAE 137

Query: 67  RTVEQPE-------NNVEVKKTIAKGLPP-----------------------GWIREIRV 103
            T   P+       N V    T+    PP                       GWI  I  
Sbjct: 138 NTYFNPDHFEGQGSNRVTRTATVPPPPPPPLDFDFKNPPDKVSWSMANAGEEGWIPNIGD 197

BLAST of CmoCh16G001360 vs. TAIR 10
Match: AT3G46580.1 (methyl-CPG-binding domain protein 5 )

HSP 1 Score: 52.0 bits (123), Expect = 1.9e-06
Identity = 26/71 (36.62%), Postives = 42/71 (59.15%), Query Frame = 0

Query: 7  ENWLPPGWTVKVKVRKSGKK----DKYYFEPSSQMRFNSRAEVFRYLKTPAINHPESEGS 66
          +NWLPP W  +++VR SG K    DK+Y+EP +  +F S+ EV  YL+      P+ +  
Sbjct: 32 DNWLPPDWRTEIRVRTSGTKAGTVDKFYYEPITGRKFRSKNEVLYYLEH---GTPKKKSV 91

Query: 67 RTVEQPENNVE 74
          +T E  +++ E
Sbjct: 92 KTAENGDSHSE 99

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTJ81.0e-0938.26Methyl-CpG-binding domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LTJ14.9e-0730.00Methyl-CpG-binding domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9SNC02.7e-0536.62Methyl-CpG-binding domain-containing protein 5 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1EAU00.0e+0095.39methyl-CpG-binding domain-containing protein 13-like isoform X1 OS=Cucurbita mos... [more]
A0A6J1E5Z51.0e-29794.99methyl-CpG-binding domain-containing protein 13-like isoform X2 OS=Cucurbita mos... [more]
A0A6J1E5B71.7e-29295.09methyl-CpG-binding domain-containing protein 13-like isoform X3 OS=Cucurbita mos... [more]
A0A6J1J2L91.7e-28988.55methyl-CpG-binding domain-containing protein 13-like isoform X1 OS=Cucurbita max... [more]
A0A6J1JB168.5e-27388.01methyl-CpG-binding domain-containing protein 13-like isoform X2 OS=Cucurbita max... [more]
Match NameE-valueIdentityDescription
XP_022923130.10.0e+0095.39methyl-CpG-binding domain-containing protein 13-like isoform X1 [Cucurbita mosch... [more]
KAG7014774.10.0e+0096.21DNA mismatch repair protein MSH3, partial [Cucurbita argyrosperma subsp. argyros... [more]
KAG6576730.16.8e-30994.20Methyl-CpG-binding domain-containing protein 13, partial [Cucurbita argyrosperma... [more]
XP_022923131.12.1e-29794.99methyl-CpG-binding domain-containing protein 13-like isoform X2 [Cucurbita mosch... [more]
XP_023552462.11.3e-29490.20methyl-CpG-binding domain-containing protein 13-like isoform X2 [Cucurbita pepo ... [more]
Match NameE-valueIdentityDescription
AT5G52230.17.5e-1138.26methyl-CPG-binding domain protein 13 [more]
AT5G52230.27.5e-1138.26methyl-CPG-binding domain protein 13 [more]
AT5G59380.13.5e-0830.00methyl-CPG-binding domain 6 [more]
AT3G46580.11.9e-0636.62methyl-CPG-binding domain protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001739Methyl-CpG DNA bindingPFAMPF01429MBDcoord: 6..51
e-value: 2.9E-8
score: 33.3
coord: 76..128
e-value: 1.6E-7
score: 30.9
IPR001739Methyl-CpG DNA bindingPROSITEPS50982MBDcoord: 72..147
score: 15.109206
IPR001739Methyl-CpG DNA bindingPROSITEPS50982MBDcoord: 1..70
score: 13.375299
NoneNo IPR availableGENE3D3.30.890.10coord: 2..55
e-value: 2.5E-14
score: 54.8
NoneNo IPR availableGENE3D3.30.890.10coord: 56..174
e-value: 6.6E-14
score: 53.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 248..304
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..264
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..286
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 342..383
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..304
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..383
NoneNo IPR availablePANTHERPTHR34067:SF15METHYL-CPG-BINDING DOMAIN-CONTAINING PROTEIN 13coord: 1..579
NoneNo IPR availableCDDcd00122MBDcoord: 82..132
e-value: 8.34001E-11
score: 55.7933
NoneNo IPR availableCDDcd00122MBDcoord: 7..50
e-value: 1.19766E-10
score: 55.4081
IPR038945Methyl-CpG-binding domain-containing protein 13-likePANTHERPTHR34067OS04G0193200 PROTEINcoord: 1..579
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 78..130
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 7..52

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G001360.1CmoCh16G001360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding