CmoCh16G001080 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G001080
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Beta-carotene hydroxylase 2
Location	Cmo_Chr16: 494904 .. 497541 (+)
RNA-Seq Expression	CmoCh16G001080
Synteny	CmoCh16G001080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGTATATAAACGAAAGAAGGGCAGCAAACTTCCAGTTTGTCCGCTCAGAGTAATTTAGTCCCCCACTTCACTGACGAAAACTTGTGGTCTCTGTTCCTTCAACCTCTATTAAAGCCCTCTCTCCGATAAGCTTCTCCCTCTTCCACCGTCTTTTCACTTTTCACCTTCATCTCTCCCATGGCGGCCGCCATCTCCGGCGCCTTAGGACCCAGACCACTCCATCTCTTCCTTCCTTCCTCTCATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCTTCCGGAACAGTAGAATCCAGCAGAAGATAAGGAGAAAAACGCTGTTCACTGTCTGCGTGCTCGTTGAGGATCAAAATAGCTCTGGCGAGGTGGAGAGTCTTTCCGATGAAGAACCGCCGATTGTAGTCCCCCAGATCCCATCGCCTTATGTTGCACAAAGATTGGCAAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGTCCTCTCTTTCTCTATCGAGAAACTTTAATTGTTTTTGTTCTCATTGGCGCCGTATTCTTCTGATTGAATATTGAATTGCGATTGTTTAATTTGGCGGTGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTACGTTTTCGATTTCATCTTTGTATTCTCTACTTCTCGCGAAGTGTTCGATTTGCTATAAGCTAATCTTTCACAATCAATTTGGGAATATCAGGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACTCTTCCTTGTGGCATATGCACGAGGTTTCTAATCCTTCTTCTCTTTAGAACATTTGAATCCACTGTGAAGCTTAGGGAAAATAGATTCGTGACGCTTGGACTTGTAATTGCCGCAGTCGCACCATTTCTAGACTAATCCTTTCTTCATAGATTATTCTTTATTGGCTGCTGAGAAATTTAATTAACAATCTCCGTTTTCCATGTTCAATCTCGAAGCAAACAAACAACATTTAATTTATTGTTTAAAAAAGTCGTTGACGCTTGGACTTTGTTGATCTTGATTATTACAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAATGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCCGGTCTCTGCTTCGGCGCTGTAAATCTCTCTCTCTCTCTCTCTCTCTCATAATCTTTCAGAATCTCAGTTTATTCATATTCCAACTGTTCAATGGTTATTTAATCACTGGGGATTAGCGCTAATGATATGTTAAATGACAAAATTAGCCTCAGAAAATGTGGTATTGAGAAAGGTTTGACACGCTGGCGGGTTGTGGCAGGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGGTAAATTTATATTTCTATAATTCTATTTATTTATTTAAATCTTATTTTGATCTCAAATTTGATACTCTCTTTACTCCCTACTGTGTTGACTTTTCATTAATGATCTAACAAAATCTCTATTAGTAGATTTCTGCTAATTAATTATATGAAAAATGGGTTAAATAAAAAATTCAATGCTCCTTTTTTCTAAATTTATGAATATCGAGAAGCTAGACATCAGCGTAGAATAGACTTAGATACAAAAATATAATTTTAAGTCAGCTGATTAATGGGATCTCAGTAATAATTTAAATGACTATTCGAATAATAATAATTGTAATAAGAAAATAGCTATGGAATTTGGAACTATATTAGAATTTTTAGACTTTCCATTTTTTTTATTAATGTCACATTAAATTATGTTTTTTAGGGTTAAAATTAAAATATTATCTCTGATTTGAATATTGAAGCTTATGAGTTTTTTTTTTCTTGGCTTAAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCATATGGACTGTTTTTAGGTCCGAAGGTCAGTTTCGGGTCTATCTTTCTATCTATATACACACATACATGTATATACATACATACATACATACACATATATATATATATATATTAAAAGTTTAATAAATTAATTTTGTAAAATTTAAATGATGGGTGCAGGAATTAGAGGAAGTGGGAGGGCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATTGACGACAACAAAATCAATCGACGGCTCTTGATGAACCAATATTGTGTGAAAATGAGACGACGAAAAAAGGGGGGAAATACAGGCCTCCCTGAACAGTAACTTTTCTGTTTTTTAACATTGAAAAGTGAAATATTATGTAGAACAGGGGGTGGTATTTTTTTTTTTATCTTCTTTTACTGTATGAGGCAAAGCGGCAGGGAAATGAGAGTTGAGTGAATTGGGAGGAAGATTTTTACTGTGACTCGCTCTCTACCGTTCCGGTTTCTGTGACAGTAAAGAAAATTTGGTTGGGGATAG

mRNA sequence

TCGTATATAAACGAAAGAAGGGCAGCAAACTTCCAGTTTGTCCGCTCAGAGTAATTTAGTCCCCCACTTCACTGACGAAAACTTGTGGTCTCTGTTCCTTCAACCTCTATTAAAGCCCTCTCTCCGATAAGCTTCTCCCTCTTCCACCGTCTTTTCACTTTTCACCTTCATCTCTCCCATGGCGGCCGCCATCTCCGGCGCCTTAGGACCCAGACCACTCCATCTCTTCCTTCCTTCCTCTCATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCTTCCGGAACAGTAGAATCCAGCAGAAGATAAGGAGAAAAACGCTGTTCACTGTCTGCGTGCTCGTTGAGGATCAAAATAGCTCTGGCGAGGTGGAGAGTCTTTCCGATGAAGAACCGCCGATTGTAGTCCCCCAGATCCCATCGCCTTATGTTGCACAAAGATTGGCAAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACTCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAATGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCCGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCATATGGACTGTTTTTAGGTCCGAAGGAATTAGAGGAAGTGGGAGGGCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATTGACGACAACAAAATCAATCGACGGCTCTTGATGAACCAATATTGTGTGAAAATGAGACGACGAAAAAAGGGGGGAAATACAGGCCTCCCTGAACAGTAACTTTTCTGTTTTTTAACATTGAAAAGTGAAATATTATGTAGAACAGGGGGTGGTATTTTTTTTTTTATCTTCTTTTACTGTATGAGGCAAAGCGGCAGGGAAATGAGAGTTGAGTGAATTGGGAGGAAGATTTTTACTGTGACTCGCTCTCTACCGTTCCGGTTTCTGTGACAGTAAAGAAAATTTGGTTGGGGATAG

Coding sequence (CDS)

ATGGCGGCCGCCATCTCCGGCGCCTTAGGACCCAGACCACTCCATCTCTTCCTTCCTTCCTCTCATCTCTCCCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCTTCCGGAACAGTAGAATCCAGCAGAAGATAAGGAGAAAAACGCTGTTCACTGTCTGCGTGCTCGTTGAGGATCAAAATAGCTCTGGCGAGGTGGAGAGTCTTTCCGATGAAGAACCGCCGATTGTAGTCCCCCAGATCCCATCGCCTTATGTTGCACAAAGATTGGCAAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACTCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAATGCCGTGCCCGCCATAGCTCTCCTCTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCCGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCATATGGACTGTTTTTAGGTCCGAAGGAATTAGAGGAAGTGGGAGGGCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATTGACGACAACAAAATCAATCGACGGCTCTTGA

Protein sequence

MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVEDQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKLTTTKSIDGS

Homology

[Close]

BLAST of CmoCh16G001080 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 441.0 bits (1133), Expect = 1.1e-122
Identity = 217/287 (75.61%), Postives = 243/287 (84.67%), Query Frame = 0


Query: 25  PKPRTPFLFPPSVFR-NSRIQQKIRRKTLFTVCVLVEDQNSSGEVESLSDEEPPIVVPQI 84
           P P TP ++P + F  N     + RR+  FTVC ++ED     + E+  ++    +  QI
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 86

Query: 85  PSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  +A++LARK+SERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 87  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 146

Query: 145 SLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLS 204
           +LSVGAAVGMEFWARWAH+ALWH+SLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 
Sbjct: 147 ALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLD 206

Query: 205 YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLH 264
           YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANVPY RKVAAAH LH
Sbjct: 207 YGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSLH 266

Query: 265 HSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKLTTTKSIDGS 311
           HS+KFNGVPYGLFLGPKELEEVGG EELEKE+NRR     T+ I GS
Sbjct: 267 HSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRR-----TRYIKGS 308

[Close]

BLAST of CmoCh16G001080 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.7e-115
Identity = 212/289 (73.36%), Postives = 237/289 (82.01%), Query Frame = 0


Query: 20  SSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVEDQNSSGEVESLSDEEPPI- 79
           SS +S    T  +FPP  F+      + R K   TVC ++E++   G++   SD++    
Sbjct: 30  SSPVSTSTPTLVVFPP--FKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVASDDDDGAG 89

Query: 80  -VVPQ------IPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEG 139
            V  Q        +  +AQ+LARK+SERFTYLVAAVMSSFGITSMAV++VYYRF WQMEG
Sbjct: 90  EVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQMEG 149

Query: 140 GEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFA 199
           GEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWH+SLWHMHESHHKPREGPFELND+FA
Sbjct: 150 GEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPFELNDIFA 209

Query: 200 IINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP 259
           IINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIA+VP
Sbjct: 210 IINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIADVP 269

Query: 260 YFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 301
           YFR+VAAAH LHHSDKFNGVPYGLFLGPKELEEVGG + LE EINRR K
Sbjct: 270 YFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRTK 316

[Close]

BLAST of CmoCh16G001080 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 2.7e-113
Identity = 216/317 (68.14%), Postives = 244/317 (76.97%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFL---FPPSVFRNSRIQQKIRRKTLFTVCV 60
           MAA +S A+  +PLH    SS    + R P     F PS+           R   F+VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-----------RFKRFSVCY 60

Query: 61  LVEDQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLA----RKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +E+    E       I + Y+A RLA    RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEK 300
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 301 EINRRIKLTTTKSIDGS 311
           EI+RRIK     S  GS
Sbjct: 301 EISRRIKSYKKASGSGS 306

[Close]

BLAST of CmoCh16G001080 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 7.3e-111
Identity = 207/303 (68.32%), Postives = 242/303 (79.87%), Query Frame = 0


Query: 1   MAAAISG-ALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLV 60
           MAA +S  A+  +PL+    SS  +  P +  +FPPS+  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVESLSDEEPPIVVPQI-PSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAV 120
           E++  S  ++  +  E      +I  +  + ++  +K+SERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHK 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRR 300
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 295

Query: 301 IKL 302
           IKL
Sbjct: 301 IKL 295

[Close]

BLAST of CmoCh16G001080 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 399.1 bits (1024), Expect = 4.7e-110
Identity = 207/312 (66.35%), Postives = 241/312 (77.24%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHL----FLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVC 60
           MAA IS +   R + L    F    + +  P  P LF   +  N     + RRK     C
Sbjct: 1   MAAEISISASSRAICLQRNPFPAPKYFATAP--PLLFFSPLTCNLDAILRSRRKPRLAAC 60

Query: 61  VLVED-------QNSSGEVESLSDE-EPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVM 120
            +++D            + E++ DE E      +  +  +A++ ARK+SERFTYLVAAVM
Sbjct: 61  FVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEEKSLAVRLAEKFARKKSERFTYLVAAVM 120

Query: 121 SSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSS 180
           SS GITSMAV++VYYRF WQMEGGE+PFSEMF TF+L+ GAA+GME+WARWAHRALWH+S
Sbjct: 121 SSLGITSMAVISVYYRFSWQMEGGEMPFSEMFCTFALAFGAAIGMEYWARWAHRALWHAS 180

Query: 181 LWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGM 240
           LWHMHESHH+PREGPFELND+FAIINAVPAIALLS+GF HKGL+PGLCFGAGLGITVFGM
Sbjct: 181 LWHMHESHHRPREGPFELNDIFAIINAVPAIALLSFGFNHKGLIPGLCFGAGLGITVFGM 240

Query: 241 AYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQ 300
           AYMFVHDGLVHKRFPVGPIANVPYF++VAAAHQLHHSDKF+GVPYGLFLGPKELEEVG  
Sbjct: 241 AYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQLHHSDKFDGVPYGLFLGPKELEEVGVL 300

[Close]

BLAST of CmoCh16G001080 vs. ExPASy TrEMBL
Match: A0A6J1E4L5 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430578 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 7.9e-177
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
           LTTTKSIDGS
Sbjct: 301 LTTTKSIDGS 310

[Close]

BLAST of CmoCh16G001080 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.0e-171
Identity = 299/310 (96.45%), Postives = 306/310 (98.71%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAA+S ALGP+PLHLFL SSHLS KPRTPFLFPPSVFRNSRIQ+K+RRKTLFTVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVE+LSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWHSS+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
           LTTTKSI+GS
Sbjct: 301 LTTTKSINGS 310

[Close]

BLAST of CmoCh16G001080 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 281/310 (90.65%), Postives = 294/310 (94.84%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAA +S A  P+P HLFL SSHLSPKP +PFLFPPSVFRNSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
            QNSSG+V++LSDEE PIVVPQIPSP+V++RLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
            T  KSIDGS
Sbjct: 301 WTARKSIDGS 310

[Close]

BLAST of CmoCh16G001080 vs. ExPASy TrEMBL
Match: A0A1S3AZ37 (beta-carotene hydroxylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484113 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.7e-160
Identity = 283/310 (91.29%), Postives = 292/310 (94.19%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAA+S AL P+P HLFL SSHLS KP TPFLFPPSVF NSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEV SLSDEE PIVVPQIPSP+V++RLARK+SERFTYLVAA+MSSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
            T  KS  GS
Sbjct: 301 WTAPKSNYGS 310

[Close]

BLAST of CmoCh16G001080 vs. ExPASy TrEMBL
Match: A0A088CIY4 (Beta-carotene hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=CHXB PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.7e-160
Identity = 283/310 (91.29%), Postives = 292/310 (94.19%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAA+S AL P+P HLFL SSHLS KP TPFLFPPSVF NSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEV SLSDEE PIVVPQIPSP+V++RLARK+SERFTYLVAA+MSSFGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
            T  KS  GS
Sbjct: 301 WTAPKSNYGS 310

[Close]

BLAST of CmoCh16G001080 vs. NCBI nr
Match: XP_022922621.1 (beta-carotene hydroxylase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 629.4 bits (1622), Expect = 1.6e-176
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
           LTTTKSIDGS
Sbjct: 301 LTTTKSIDGS 310

[Close]

BLAST of CmoCh16G001080 vs. NCBI nr
Match: KAG7014750.1 (Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.5 bits (1591), Expect = 6.4e-173
Identity = 303/310 (97.74%), Postives = 308/310 (99.35%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAA+S ALGP+PLHLFL SSHLSPKPRTPFLFPPSVFRNSRIQ+KIRRKTLFTVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQN+SGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNNSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
           LTTTKSI+GS
Sbjct: 301 LTTTKSINGS 310

[Close]

BLAST of CmoCh16G001080 vs. NCBI nr
Match: KAG6576699.1 (Beta-carotene 3-hydroxylase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 613.2 bits (1580), Expect = 1.2e-171
Identity = 301/310 (97.10%), Postives = 307/310 (99.03%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAA+S ALGP+PLHLFL SSHLSPKP TPFLFPPSVFRNSRIQ+KIRRKTLFTVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSPKPGTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQN+SGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNNSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELE+EINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEEEINRRIK 300

Query: 301 LTTTKSIDGS 311
           LTTTKSI+GS
Sbjct: 301 LTTTKSINGS 310

[Close]

BLAST of CmoCh16G001080 vs. NCBI nr
Match: XP_022984277.1 (beta-carotene hydroxylase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 610.1 bits (1572), Expect = 1.0e-170
Identity = 299/310 (96.45%), Postives = 306/310 (98.71%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAA+S ALGP+PLHLFL SSHLS KPRTPFLFPPSVFRNSRIQ+K+RRKTLFTVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVE+LSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWHSS+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 LTTTKSIDGS 311
           LTTTKSI+GS
Sbjct: 301 LTTTKSINGS 310

[Close]

BLAST of CmoCh16G001080 vs. NCBI nr
Match: XP_023551817.1 (beta-carotene hydroxylase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.8 bits (1571), Expect = 1.3e-170
Identity = 302/311 (97.11%), Postives = 307/311 (98.71%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60
           MAAA+S ALGP+PLHLFL SSHLSPKPRTPFLFPPSVFRNSRIQ+KIRRKTLFTVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVESLSDEEP IVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVESLSDEEPKIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG+EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGEEELEKEINRRIK 300

Query: 301 LTTTK-SIDGS 311
           LTTTK SI+GS
Sbjct: 301 LTTTKSSINGS 311

[Close]

BLAST of CmoCh16G001080 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 409.8 bits (1052), Expect = 1.9e-114
Identity = 216/317 (68.14%), Postives = 244/317 (76.97%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFL---FPPSVFRNSRIQQKIRRKTLFTVCV 60
           MAA +S A+  +PLH    SS    + R P     F PS+           R   F+VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-----------RFKRFSVCY 60

Query: 61  LVEDQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLA----RKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +E+    E       I + Y+A RLA    RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEK 300
           HDGLVHKRFPVGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 301 EINRRIKLTTTKSIDGS 311
           EI+RRIK     S  GS
Sbjct: 301 EISRRIKSYKKASGSGS 306

[Close]

BLAST of CmoCh16G001080 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 401.7 bits (1031), Expect = 5.2e-112
Identity = 207/303 (68.32%), Postives = 242/303 (79.87%), Query Frame = 0


Query: 1   MAAAISG-ALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLV 60
           MAA +S  A+  +PL+    SS  +  P +  +FPPS+  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVESLSDEEPPIVVPQI-PSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAV 120
           E++  S  ++  +  E      +I  +  + ++  +K+SERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHK 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRR 300
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 295

Query: 301 IKL 302
           IKL
Sbjct: 301 IKL 295

[Close]

BLAST of CmoCh16G001080 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 253.4 bits (646), Expect = 2.3e-67
Identity = 139/226 (61.50%), Postives = 162/226 (71.68%), Query Frame = 0


Query: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFL---FPPSVFRNSRIQQKIRRKTLFTVCV 60
           MAA +S A+  +PLH    SS    + R P     F PS+           R   F+VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-----------RFKRFSVCY 60

Query: 61  LVEDQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLA----RKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +E+    E       I + Y+A RLA    RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMH 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWH+SLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of CmoCh16G001080 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 240.0 bits (611), Expect = 2.6e-63
Identity = 146/303 (48.18%), Postives = 179/303 (59.08%), Query Frame = 0


Query: 1   MAAAISG-ALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLV 60
           MAA +S  A+  +PL+    SS  +  P +  +FPPS+  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVESLSDEEPPIVVPQI-PSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAV 120
           E++  S  ++  +  E      +I  +  + ++  +K+SERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHK 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGA                             
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGA----------------------------- 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
                                                   AGLGIT+FGMAYMFVHDGLV
Sbjct: 181 ----------------------------------------AGLGITMFGMAYMFVHDGLV 226

Query: 241 HKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRR 300
           HKRFPVGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 226

Query: 301 IKL 302
           IKL
Sbjct: 301 IKL 226

The following BLAST results are available for this feature:

BLAST of CmoCh16G001080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	1.1e-122	75.61	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	1.7e-115	73.36	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	2.7e-113	68.14	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9LTG0	7.3e-111	68.32	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	4.7e-110	66.35	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]

BLAST of CmoCh16G001080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E4L5	7.9e-177	100.00	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1J1S8	5.0e-171	96.45	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0L606	1.4e-160	90.65	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A1S3AZ37	6.7e-160	91.29	beta-carotene hydroxylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034841...	[more]
A0A088CIY4	6.7e-160	91.29	Beta-carotene hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=CHXB PE=2 SV...	[more]

BLAST of CmoCh16G001080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922621.1	1.6e-176	100.00	beta-carotene hydroxylase 2, chloroplastic-like [Cucurbita moschata]	[more]
KAG7014750.1	6.4e-173	97.74	Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argy...	[more]
KAG6576699.1	1.2e-171	97.10	Beta-carotene 3-hydroxylase, chloroplastic, partial [Cucurbita argyrosperma subs...	[more]
XP_022984277.1	1.0e-170	96.45	beta-carotene hydroxylase 2, chloroplastic-like [Cucurbita maxima]	[more]
XP_023551817.1	1.3e-170	97.11	beta-carotene hydroxylase 2, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh16G001080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	1.9e-114	68.14	beta-hydroxylase 1	[more]
AT5G52570.1	5.2e-112	68.32	beta-carotene hydroxylase 2	[more]
AT4G25700.2	2.3e-67	61.50	beta-hydroxylase 1	[more]
AT5G52570.2	2.6e-63	48.18	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 149..276 e-value: 5.7E-14 score: 52.6
None	No IPR available	PANTHER	PTHR31899:SF10	BETA-CAROTENE HYDROXYLASE	coord: 15..302
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 15..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh16G001080.1	CmoCh16G001080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity