CmoCh16G000580 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G000580
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr16: 242264 .. 245230 (-)
RNA-Seq ExpressionCmoCh16G000580
SyntenyCmoCh16G000580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGGAGTAGCCGCCACCTAATTCTCCCTTCAAAATCTCTCCCATTTCATATATATTTACTCTAAACAACCTCAACCCACCTCTGTCCTTTACCCTTTCCTCTCTCTCTGCTTCTCTATCCTCTCCTCTCCACAATGGGATACGGCAGGCTCAACGAAACCGACCCTGGCGGCACTTCCTCCGACGCACCTTCCCACCTCTCCCACCATGCCGCTTCTCCCAACCCACGGAGAAGCCGCCTTCTCCTTATCTCCCTCCTCTCTCTCATTCTCATCGTCGCTGCCGCTGTCTCTGCCACGTTTCTCGTCGGCCTCCGAACCGCCTCCTCCGTCCGATCCGACCCCAATCTCCCTCGAAAACCGACTCAGGCCATTTCCAAGGCCTGCAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGGATCTCTGAACGCCAATGAACAGGATCTCGTCCACATTTCCTTTAACATGACTCTCCAGCATCTCAACAAAGCCCTCTACTTCTCCTCTGGAATCTCCTCTCTGCAAATGGATCTTCGCGTACGCTCCGCCTACGATGCCTGTCTTGAACTCCTCGACGACTCTGTCGACGCCCTTACCCGCTCCTTGCAGTCCGTCGCGCCCTCTTCCTCCAACGGCGGTCCTCAACGCCTTGGCTCCTCCGATGACGTCATCACCTGGCTCAGCGCTGCCCTTACCAATCAAGACACGTGTACGGACGGATTCTCCCAACTCTCCGGCTCTGTCAAAGACCAAATGGCCGATAAACTCCACAATTTGTCCGAACTCGTCAGCAATTGCTTGGCACTGTTTTCCGGCAGTGTAACGAGCGATTTCTCCGGAGTGCCGATTCAGAACAAGCGCCGGTTGATGAACGCCGGCGAAAACGAGGATGCTTCCGGTAACTTCCCCGGCTGGATGAGTCGGAGAGAGAGGAGGTTACTGACGTTGCCGGTGGGGGCGTTACAGGCCGATATTGTGGTTTCTCAGAACGGAAGCGGAACGGTGAAGACGATCGCGGAAGCGATCAAAAAGGCGCCGCAGTACAGTAACAGAAGGACGATCATATACGTGATGGCAGGAAGGTAATTTATTAATTTCGGTGTTCTGATTTATTTATTTATTTTAACCGAACCGCGTTTCGTTTGACTTGTCACTTGCGTGTCAATATCGTGAACTGTTTTCACGTGATTTGGAAAAAGCATGCTCCATACGAATTTGGCTCACGGTGGATTTACGGAAATACCCTTGTTCGTCCTTTCTGGCAGGTACGAAGAGAAAAATCTGAAGGTGGGGAGGAAGAAATGGAACCTGATGTTTGTTGGGGATGGCAAGGGTAAAACTGTCATTTCTGGTGGTAAGAGCATCTTTGACAACGTCACAACCTTTCACACGGCGTCGTTTGGTAATAAATCTAACTTTCATTTATTTATTACACTCACTTTCTCTCTTTAATTTTTAATTTTTAATTTTTTGGCCATAATAATTTAAGAGGTCAGAAGAATTCCTCGTTCAAATTATAAAAAAAAAAAAAAACACCCCACGTGCTAATGCAGCCTTGATTGTACTCGCACGTGCCGTCTCGTAATCGTGTTCTTCCTTTTGCGAGATTTTCCCAAACTACCCTTCTATAATTGCCTCAATTAGTGTCTATATTTTGAAAAAGAAAATAAATTGTCTGTGGGCAACAAGTGGTGGTCAGAGTCATTTGCCACAATTTCCTAATTATTTGTCTAACTAATAATATATATATAGTGTTATAATTCCCACTTCAAATTAATCGTCTTTTTGTCCATTGTCAGTAATTTATTATTCTTCTCAAATATATGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTAAAAACAAATTCTTGTGATCACAGCGGCCACCGGCGCTGGTATAATCTTAAGGGACATGACATTTGAGAATTGGGCGGGGCCTGGCCGGCACCAGGCAGTTGCACTTCGAGTTGGCGGCGACCACGCCGTGGTGTACCGCTGCAACATAATTGGCTATCAAGACACTCTCTACGTCCATTCCAACCGCCAGTTCTACCGGGAGTGTGACATTTATGGGACGGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATCTACGCCCGAAAGGCAATGGACCTTCAAAAGAACACACTCACCGCCCAAAACCGGAAGGATCCCAATCAGAACACTGGAATCTCCATCCATGCGTGTCGGATTTTGGCCGCCTCCGATCTGGCGTCGTCCAATAGCAGCAACCCGACCTATTTGGGGCGTCCCTGGAAATTATACTCCAGAGTCGTGTTCATGCTATCGTACATAGGGAGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAATGCCACGTTTGCGCTGGACACGCTGTATTATGGGGAGTATATGAATTACGGACCGGGTGGGGCGGTGGGTCAAAGGGTGACATGGCCTGGGTATCGGGTCATAACTTCCACGGTGGAGGCGAGTAAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGTTGCCGTCCACGGGAGTTGCATTCCTGGGAGGGTTAAATGTGTAATTTGGGTAATTGTATTATATAAGGTGGTAATAAAGAAAGAAGAAAAAAAAAAGTGTGGGCATAAAATACGTATAATTAATAGTGGAGTTTTCTTTTTTCCATATATAATTAATAATATATGTTTCATTCTTTTTTCCTCTGAAATTGTATTGTAACTGAATATTATCTGATTTTTTATGATATTAATAAGGCATTAATATTTTGCAA

mRNA sequence

TGTGGAGTAGCCGCCACCTAATTCTCCCTTCAAAATCTCTCCCATTTCATATATATTTACTCTAAACAACCTCAACCCACCTCTGTCCTTTACCCTTTCCTCTCTCTCTGCTTCTCTATCCTCTCCTCTCCACAATGGGATACGGCAGGCTCAACGAAACCGACCCTGGCGGCACTTCCTCCGACGCACCTTCCCACCTCTCCCACCATGCCGCTTCTCCCAACCCACGGAGAAGCCGCCTTCTCCTTATCTCCCTCCTCTCTCTCATTCTCATCGTCGCTGCCGCTGTCTCTGCCACGTTTCTCGTCGGCCTCCGAACCGCCTCCTCCGTCCGATCCGACCCCAATCTCCCTCGAAAACCGACTCAGGCCATTTCCAAGGCCTGCAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGGATCTCTGAACGCCAATGAACAGGATCTCGTCCACATTTCCTTTAACATGACTCTCCAGCATCTCAACAAAGCCCTCTACTTCTCCTCTGGAATCTCCTCTCTGCAAATGGATCTTCGCGTACGCTCCGCCTACGATGCCTGTCTTGAACTCCTCGACGACTCTGTCGACGCCCTTACCCGCTCCTTGCAGTCCGTCGCGCCCTCTTCCTCCAACGGCGGTCCTCAACGCCTTGGCTCCTCCGATGACGTCATCACCTGGCTCAGCGCTGCCCTTACCAATCAAGACACGTGTACGGACGGATTCTCCCAACTCTCCGGCTCTGTCAAAGACCAAATGGCCGATAAACTCCACAATTTGTCCGAACTCGTCAGCAATTGCTTGGCACTGTTTTCCGGCAGTGTAACGAGCGATTTCTCCGGAGTGCCGATTCAGAACAAGCGCCGGTTGATGAACGCCGGCGAAAACGAGGATGCTTCCGGTAACTTCCCCGGCTGGATGAGTCGGAGAGAGAGGAGGTTACTGACGTTGCCGGTGGGGGCGTTACAGGCCGATATTGTGGTTTCTCAGAACGGAAGCGGAACGGTGAAGACGATCGCGGAAGCGATCAAAAAGGCGCCGCAGTACAGTAACAGAAGGACGATCATATACGTGATGGCAGGAAGGTACGAAGAGAAAAATCTGAAGGTGGGGAGGAAGAAATGGAACCTGATGTTTGTTGGGGATGGCAAGGGTAAAACTGTCATTTCTGGTGGTAAGAGCATCTTTGACAACGTCACAACCTTTCACACGGCGTCGTTTGCGGCCACCGGCGCTGGTATAATCTTAAGGGACATGACATTTGAGAATTGGGCGGGGCCTGGCCGGCACCAGGCAGTTGCACTTCGAGTTGGCGGCGACCACGCCGTGGTGTACCGCTGCAACATAATTGGCTATCAAGACACTCTCTACGTCCATTCCAACCGCCAGTTCTACCGGGAGTGTGACATTTATGGGACGGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATCTACGCCCGAAAGGCAATGGACCTTCAAAAGAACACACTCACCGCCCAAAACCGGAAGGATCCCAATCAGAACACTGGAATCTCCATCCATGCGTGTCGGATTTTGGCCGCCTCCGATCTGGCGTCGTCCAATAGCAGCAACCCGACCTATTTGGGGCGTCCCTGGAAATTATACTCCAGAGTCGTGTTCATGCTATCGTACATAGGGAGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAATGCCACGTTTGCGCTGGACACGCTGTATTATGGGGAGTATATGAATTACGGACCGGGTGGGGCGGTGGGTCAAAGGGTGACATGGCCTGGGTATCGGGTCATAACTTCCACGGTGGAGGCGAGTAAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGTTGCCGTCCACGGGAGTTGCATTCCTGGGAGGGTTAAATGTGTAATTTGGGTAATTGTATTATATAAGGTGGTAATAAAGAAAGAAGAAAAAAAAAAGTGTGGGCATAAAATACGTATAATTAATAGTGGAGTTTTCTTTTTTCCATATATAATTAATAATATATGTTTCATTCTTTTTTCCTCTGAAATTGTATTGTAACTGAATATTATCTGATTTTTTATGATATTAATAAGGCATTAATATTTTGCAA

Coding sequence (CDS)

ATGGGATACGGCAGGCTCAACGAAACCGACCCTGGCGGCACTTCCTCCGACGCACCTTCCCACCTCTCCCACCATGCCGCTTCTCCCAACCCACGGAGAAGCCGCCTTCTCCTTATCTCCCTCCTCTCTCTCATTCTCATCGTCGCTGCCGCTGTCTCTGCCACGTTTCTCGTCGGCCTCCGAACCGCCTCCTCCGTCCGATCCGACCCCAATCTCCCTCGAAAACCGACTCAGGCCATTTCCAAGGCCTGCAGCCGAACTCGCTTCCCGACTCTCTGTGTCAACTCGCTCCTCGATTTCCCTGGATCTCTGAACGCCAATGAACAGGATCTCGTCCACATTTCCTTTAACATGACTCTCCAGCATCTCAACAAAGCCCTCTACTTCTCCTCTGGAATCTCCTCTCTGCAAATGGATCTTCGCGTACGCTCCGCCTACGATGCCTGTCTTGAACTCCTCGACGACTCTGTCGACGCCCTTACCCGCTCCTTGCAGTCCGTCGCGCCCTCTTCCTCCAACGGCGGTCCTCAACGCCTTGGCTCCTCCGATGACGTCATCACCTGGCTCAGCGCTGCCCTTACCAATCAAGACACGTGTACGGACGGATTCTCCCAACTCTCCGGCTCTGTCAAAGACCAAATGGCCGATAAACTCCACAATTTGTCCGAACTCGTCAGCAATTGCTTGGCACTGTTTTCCGGCAGTGTAACGAGCGATTTCTCCGGAGTGCCGATTCAGAACAAGCGCCGGTTGATGAACGCCGGCGAAAACGAGGATGCTTCCGGTAACTTCCCCGGCTGGATGAGTCGGAGAGAGAGGAGGTTACTGACGTTGCCGGTGGGGGCGTTACAGGCCGATATTGTGGTTTCTCAGAACGGAAGCGGAACGGTGAAGACGATCGCGGAAGCGATCAAAAAGGCGCCGCAGTACAGTAACAGAAGGACGATCATATACGTGATGGCAGGAAGGTACGAAGAGAAAAATCTGAAGGTGGGGAGGAAGAAATGGAACCTGATGTTTGTTGGGGATGGCAAGGGTAAAACTGTCATTTCTGGTGGTAAGAGCATCTTTGACAACGTCACAACCTTTCACACGGCGTCGTTTGCGGCCACCGGCGCTGGTATAATCTTAAGGGACATGACATTTGAGAATTGGGCGGGGCCTGGCCGGCACCAGGCAGTTGCACTTCGAGTTGGCGGCGACCACGCCGTGGTGTACCGCTGCAACATAATTGGCTATCAAGACACTCTCTACGTCCATTCCAACCGCCAGTTCTACCGGGAGTGTGACATTTATGGGACGGTGGATTTCATCTTCGGCAATGCGGCGGTGGTGTTTCAGAACTGCAGCATCTACGCCCGAAAGGCAATGGACCTTCAAAAGAACACACTCACCGCCCAAAACCGGAAGGATCCCAATCAGAACACTGGAATCTCCATCCATGCGTGTCGGATTTTGGCCGCCTCCGATCTGGCGTCGTCCAATAGCAGCAACCCGACCTATTTGGGGCGTCCCTGGAAATTATACTCCAGAGTCGTGTTCATGCTATCGTACATAGGGAGCCACGTGCACCCACGCGGTTGGCTGGAGTGGAATGCCACGTTTGCGCTGGACACGCTGTATTATGGGGAGTATATGAATTACGGACCGGGTGGGGCGGTGGGTCAAAGGGTGACATGGCCTGGGTATCGGGTCATAACTTCCACGGTGGAGGCGAGTAAGTTCACGGTGGCGCAGTTCATTTACGGCTCGTCGTGGTTGCCGTCCACGGGAGTTGCATTCCTGGGAGGGTTAAATGTGTAA

Protein sequence

MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV
Homology
BLAST of CmoCh16G000580 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 1.0e-225
Identity = 408/611 (66.78%), Postives = 479/611 (78.40%), Query Frame = 0

Query: 1   MGYGRLNETDPGG---TSSDAPSHLSHHAASPNP----RRSRLLLISLLSLILIVAAAVS 60
           MGY RL  +   G   TS+     L+  + S  P    RRS+  L+ + S++L ++  ++
Sbjct: 1   MGYERLGPSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLV-VSSIVLAISLILA 60

Query: 61  ATFLVGLRTASSV-RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNA-NEQDL 120
           A    G+R+   + +S P L RKP+QAISKAC  TRFP LCV+SL+DFPGSL A + +DL
Sbjct: 61  AAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDL 120

Query: 121 VHISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSS 180
           +H++ NMTL H + ALY S+ +S + M  R RSAYD+C+ELLDDSVDAL+R+L SV  SS
Sbjct: 121 IHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSS 180

Query: 181 SNGGPQRLGSSDDVITWLSAALTNQDTCTDGFSQL-SGSVKDQMADKLHNLSELVSNCLA 240
           +   PQ      DV TWLSAALTN DTCT+GF  +  G VKD M   L NLSELVSNCLA
Sbjct: 181 AK--PQ------DVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLA 240

Query: 241 LFSGSVT-SDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVV 300
           +FS S    DF+GVPIQN RRL+   E E+    FP WM  +ER +L +PV  +QADI+V
Sbjct: 241 IFSASHDGDDFAGVPIQN-RRLLGVEEREE---KFPRWMRPKEREILEMPVSQIQADIIV 300

Query: 301 SQNGSGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTV 360
           S++G+GT KTI+EAIKKAPQ S RR IIYV AGRYEE NLKVGRKK NLMFVGDGKGKTV
Sbjct: 301 SKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTV 360

Query: 361 ISGGKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCN 420
           ISGGKSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+G DHAV+YRCN
Sbjct: 361 ISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCN 420

Query: 421 IIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNR 480
           IIGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNT+TAQNR
Sbjct: 421 IIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNR 480

Query: 481 KDPNQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWL 540
           KDPNQNTGISIHA R+LAASDL ++N S  TYLGRPWKL+SR V+M+SYIG HVH RGWL
Sbjct: 481 KDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWL 540

Query: 541 EWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPS 600
           EWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI ST EA++FTVA+FIYGSSWLPS
Sbjct: 541 EWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPS 598

BLAST of CmoCh16G000580 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 8.3e-220
Identity = 385/602 (63.95%), Postives = 464/602 (77.08%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           MGY RL  + P   +   P+      + P  ++     + L +L ++V   V      G+
Sbjct: 1   MGYDRLGPSGPSNPNQKDPA-----TSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGI 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           R   S +++P L RKPTQAIS+ CS++ +P LC+++LLDFPGSL A+E +L+HISFN TL
Sbjct: 61  RAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180
           Q  +KALY SS I+  QM  RVRSAYD+CLELLDDSVDALTR+L SV   S +       
Sbjct: 121 QKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGD------E 180

Query: 181 SSDDVITWLSAALTNQDTCTDGFSQL---SGSVKDQMADKLHNLSELVSNCLALFSGSVT 240
           S  DV+TWLS+A+TN DTCTDGF ++    G VKDQ+   + +LSE+VSNCLA+F+G V 
Sbjct: 181 SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKV- 240

Query: 241 SDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 300
            D SGVP+ N R+L+   E E+     P W+ R +R LL  P  A+QADI VS++GSGT 
Sbjct: 241 KDLSGVPVVNNRKLLGTEETEE----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTF 300

Query: 301 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIF 360
           KTIAEAIKKAP++S+RR +IYV AGRYEE+NLKVGRKK NLMF+GDGKGKTVI+GGKSI 
Sbjct: 301 KTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIA 360

Query: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 420
           D++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVGGDHAVVYRCNIIGYQD L
Sbjct: 361 DDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDAL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTG 480
           YVHSNRQF+REC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK T+TAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 540
           ISIHAC++LA  DL +S  S PTYLGRPWKLYSRVV+M+S +G H+ PRGWLEWN  FAL
Sbjct: 481 ISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFAL 540

Query: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           D+LYYGEYMN G G  +GQRV WPGY VITSTVEASKFTVAQFI GSSWLPSTGV+F  G
Sbjct: 541 DSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSG 586

BLAST of CmoCh16G000580 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 9.4e-123
Identity = 227/464 (48.92%), Postives = 296/464 (63.79%), Query Frame = 0

Query: 139 DLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLGS-SDDVITWLSAALTNQD 198
           D R+ +A   CL+LLD S D L  SL   A  +  G     G  S D+ TWLSAAL NQD
Sbjct: 83  DFRLANAISDCLDLLDFSADELNWSLS--ASQNQKGKNNSTGKLSSDLRTWLSAALVNQD 142

Query: 199 TCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDFSGVPIQNKRRLMNAGEN 258
           TC++GF   +  V+  ++  L  ++ LV   L                    ++      
Sbjct: 143 TCSNGFEGTNSIVQGLISAGLGQVTSLVQELLT-------------------QVHPNSNQ 202

Query: 259 EDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTIAEAIKKAPQYSNRRTII 318
           +  +G  P W+  ++R+L  L    +  D +V+Q+G+G    + +A+  AP YS RR +I
Sbjct: 203 QGPNGQIPSWVKTKDRKL--LQADGVSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVI 262

Query: 319 YVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAATGAGIIL 378
           Y+  G Y+E N+++ +KKWNLM +GDG   T+ISG +S  D  TTF +A+FA +G G I 
Sbjct: 263 YIKRGTYKE-NVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSATFAVSGRGFIA 322

Query: 379 RDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDF 438
           RD+TFEN AGP +HQAVALR   D +V YRCNI GYQDTLY H+ RQFYR+C I GTVDF
Sbjct: 323 RDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDF 382

Query: 439 IFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISIHACRILAASDL-ASSNS 498
           IFG+A VVFQNC I A+K +  QKN++TAQ RKDPN+ TGISI  C I A SDL A+S +
Sbjct: 383 IFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITADSDLEAASVN 442

Query: 499 SNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQ 558
           S PTYLGRPWKLYSR V M S++ + + P GWLEWN  FAL++L+YGEYMNYGPG  +G 
Sbjct: 443 STPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGS 502

Query: 559 RVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 601
           RV WPGY+V   + +A  +TVAQFI G+ WLPSTGV +     V
Sbjct: 503 RVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFGV 522

BLAST of CmoCh16G000580 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 7.5e-120
Identity = 246/577 (42.63%), Postives = 350/577 (60.66%), Query Frame = 0

Query: 31  PRRSRLLLISLLSLILIVAAAVSATFLVGLRTASSVRSDPNLPRKPTQAISK-ACSRTRF 90
           P++ + L ++L + +L+VAA +    + G+ +  +   + N   +P  AI K +CS TR+
Sbjct: 24  PKKKKKLFLALFATLLVVAAVIG--IVAGVNSRKNSGDNGN---EPHHAILKSSCSSTRY 83

Query: 91  PTLCVNSLLDFPGSLN--ANEQDLVHISFNMTLQHLNKALYFSSGISSL----QMDLRVR 150
           P LC +++   P +     +++D++ +S N+T   +    YF  GI  L     +  R +
Sbjct: 84  PDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHN-YF--GIQKLLKRTNLTKREK 143

Query: 151 SAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLGSSDDVITWLSAALTNQDTCTDGF 210
            A   CLE +D+++D L ++++ +    +     +   +DD+ T +SAA+TNQ TC DGF
Sbjct: 144 VALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQ--HADDLKTLMSAAMTNQGTCLDGF 203

Query: 211 S--QLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDFSGVPIQNKRRLMNAGENEDAS 270
           S    +  V+D ++D   ++ ++ SN LA+      +D   +   N R+L+   E     
Sbjct: 204 SHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLI---EETSTV 263

Query: 271 GNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTIAEAIKKAPQYSNRRTIIYVMA 330
             +P W+S  +RRL  L   ++  ++VV+ +GSG  KT+A ++  APQ   +R II + A
Sbjct: 264 DGWPAWLSTGDRRL--LQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKA 323

Query: 331 GRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAATGAGIILRDMT 390
           G Y E N++V +K  N+MF+GDG+ +T+I+G +++ D  TTF +A+ A  G G + RD+T
Sbjct: 324 GVYRE-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDIT 383

Query: 391 FENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGN 450
           F+N AGP +HQAVALRVG D +  Y C+++ YQDTLYVHSNRQF+  C I GTVDFIFGN
Sbjct: 384 FQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGN 443

Query: 451 AAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISIHACRILAASDLASSNSSNPTY 510
           AA V QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI A SDL     S PTY
Sbjct: 444 AAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTY 503

Query: 511 LGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWP 570
           LGRPWK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ N G G     RV W 
Sbjct: 504 LGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWK 563

Query: 571 GYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 599
           G+RVITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 564 GFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CmoCh16G000580 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.8e-119
Identity = 249/585 (42.56%), Postives = 351/585 (60.00%), Query Frame = 0

Query: 23  SHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGLRTASSVRSDPNLPRKPTQAISK 82
           S+   S  P++ + L ++L + +L+VAA +    + G+ +  +   + N   +P  AI K
Sbjct: 16  SNQNISNIPKKKKKLFLALFATLLVVAAVIG--IVAGVNSRKNSGDNGN---EPHHAILK 75

Query: 83  -ACSRTRFPTLCVNSLLDFPGSLN--ANEQDLVHISFNMTLQHLNKALYFSSGISSL--- 142
            +CS TR+P LC +++   P +     +++D++ +S N+T   +    YF  GI  L   
Sbjct: 76  SSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHN-YF--GIQKLLKR 135

Query: 143 -QMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLGSSDDVITWLSAALTN 202
             +  R + A   CLE +D+++D L ++++ +    +     +   +DD+ T +SAA+TN
Sbjct: 136 TNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQ--HADDLKTLMSAAMTN 195

Query: 203 QDTCTDGFS--QLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDFSGVPIQNKRRLMN 262
           Q TC DGFS    +  V+D ++D   ++ ++ SN LA+      +D   +   N R+L  
Sbjct: 196 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLT- 255

Query: 263 AGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTIAEAIKKAPQYSNR 322
             E       +P W+S  +RRL  L   ++  + VV+ +GSG  KT+A A+  APQ   +
Sbjct: 256 --EETSTVDGWPAWLSPGDRRL--LQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTK 315

Query: 323 RTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAATGA 382
           R II + AG Y E N++V +K  N+MF+GDG+ +T+I+G +++ D  TTF +A+ A  G 
Sbjct: 316 RYIIRIKAGVYRE-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGE 375

Query: 383 GIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYG 442
           G + RD+TF+N AGP +HQAVALRVG D +  Y C+++ YQDTLYVHSNRQF+  C I G
Sbjct: 376 GFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAG 435

Query: 443 TVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISIHACRILAASDLAS 502
           TVDFIFGNAA V QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI A SDL  
Sbjct: 436 TVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKP 495

Query: 503 SNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGA 562
              S PTYLGRPWK YSR V M S I   +HP GW EW+  FAL+TL+YGE+ N G G  
Sbjct: 496 VQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAG 555

Query: 563 VGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 599
              RV W G+RVITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 556 TSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CmoCh16G000580 vs. ExPASy TrEMBL
Match: A0A6J1E487 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1)

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180
           QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180

Query: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240
           SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF
Sbjct: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240

Query: 241 SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300
           SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI
Sbjct: 241 SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300

Query: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360
           AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV
Sbjct: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360

Query: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420
           TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH
Sbjct: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420

Query: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI 480
           SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI
Sbjct: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI 480

Query: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540
           HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL
Sbjct: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600
           YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV
Sbjct: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600

BLAST of CmoCh16G000580 vs. ExPASy TrEMBL
Match: A0A6J1J930 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1)

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 590/600 (98.33%), Postives = 594/600 (99.00%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           M YGRLN+TDPGGTSSDAPS LSHHA SPNPRR RL+LISLLSLILIVAAAVSATFLVGL
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAVSPNPRRRRLILISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180
           QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180

Query: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240
           SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF
Sbjct: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240

Query: 241 SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300
           SGVPIQNKRRLMNAGENED SG+FPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI
Sbjct: 241 SGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300

Query: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360
           AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISGGKSIFDNV
Sbjct: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGGKSIFDNV 360

Query: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420
           TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH
Sbjct: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420

Query: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI 480
           SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPNQNTGISI
Sbjct: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISI 480

Query: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540
           HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL
Sbjct: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600
           YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV
Sbjct: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600

BLAST of CmoCh16G000580 vs. ExPASy TrEMBL
Match: A0A0A0L6S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 546/605 (90.25%), Postives = 570/605 (94.21%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPS----HLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATF 60
           MGYGRLNETDPGG S  +P+     LS H  +PN R +RLLLIS LSL+LIVAAAVSATF
Sbjct: 1   MGYGRLNETDPGGPSHSSPASPPFQLSSHHPTPNRRTTRLLLISFLSLLLIVAAAVSATF 60

Query: 61  LVGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF 120
           L+GLRTAS  RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF
Sbjct: 61  LIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF 120

Query: 121 NMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGP 180
           N+TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAPSSS+  P
Sbjct: 121 NVTLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTP 180

Query: 181 QRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSV 240
           QRLGSS+D+ITWLSAALTNQDTCTDGFS+LSG+VK+QMADKLHNLSELVSNCLALFSGS 
Sbjct: 181 QRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSE 240

Query: 241 TSDFSGVPIQNKRRLM-NAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSG 300
           TSDF+GVPIQNKRRLM   GENED+SG FP WM+RRERRLLTLPVG LQADIVVSQNG+G
Sbjct: 241 TSDFAGVPIQNKRRLMEEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQNGNG 300

Query: 301 TVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKS 360
           TVKTIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KS
Sbjct: 301 TVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGSKS 360

Query: 361 IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQD 420
           IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQD
Sbjct: 361 IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQD 420

Query: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQN 480
           TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNT+TAQNRKDPNQN
Sbjct: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQN 480

Query: 481 TGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATF 540
           TGISIHACRILA SDL SSN+SNPTYLGRPWKLYSR V MLSYIG+HVHPRGWLEWNATF
Sbjct: 481 TGISIHACRILATSDLESSNTSNPTYLGRPWKLYSRTVVMLSYIGNHVHPRGWLEWNATF 540

Query: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600
           ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL
Sbjct: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600

BLAST of CmoCh16G000580 vs. ExPASy TrEMBL
Match: A0A5A7U3N7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001450 PE=3 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 547/605 (90.41%), Postives = 567/605 (93.72%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSD----APSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATF 60
           MGYGRLNETDPGG S      +   LS H  +PN   +RLLLIS LSL+LIVAAAVSATF
Sbjct: 1   MGYGRLNETDPGGPSDSSSVASAFQLSAHHPTPNRSTTRLLLISFLSLLLIVAAAVSATF 60

Query: 61  LVGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF 120
           L+GLRTAS  RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF
Sbjct: 61  LIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF 120

Query: 121 NMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGP 180
           N+TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAPSSS+  P
Sbjct: 121 NITLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTP 180

Query: 181 QRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSV 240
           QRLGSS+D+ITWLSAALTNQDTCTDGFS+LSG+VK+QMADKLHNLSELVSNCLALFSGS 
Sbjct: 181 QRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSE 240

Query: 241 TSDFSGVPIQNKRRLM-NAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSG 300
           TSDF+GVPIQNKRRLM   GENED+SGNFP WM  RERRLLTLPVG LQADIVVSQNG+G
Sbjct: 241 TSDFAGVPIQNKRRLMKEEGENEDSSGNFPRWMRGRERRLLTLPVGVLQADIVVSQNGNG 300

Query: 301 TVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKS 360
           TVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KS
Sbjct: 301 TVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGSKS 360

Query: 361 IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQD 420
           IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQD
Sbjct: 361 IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQD 420

Query: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQN 480
           TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNT+TAQNRKDPNQN
Sbjct: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQN 480

Query: 481 TGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATF 540
           TGISIHACRILA SDL SSN+SNPTYLGRPWKLYSRVVFMLSYIG HVHPRGWLEWNATF
Sbjct: 481 TGISIHACRILATSDLQSSNTSNPTYLGRPWKLYSRVVFMLSYIGGHVHPRGWLEWNATF 540

Query: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600
           ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL
Sbjct: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600

BLAST of CmoCh16G000580 vs. ExPASy TrEMBL
Match: A0A6J1G0F6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.8e-279
Identity = 497/602 (82.56%), Postives = 535/602 (88.87%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           MG  RLNET P                + NPRR  L ++SLLSLILI+  AVS T L+GL
Sbjct: 1   MGCRRLNETHP----------------AANPRRGPLSVLSLLSLILIL-PAVSPTSLLGL 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           RT+    SDP LPRKPT+AISKAC+R+R P LCV+SLLDFPGSL+ANE+DLVHISFN+TL
Sbjct: 61  RTS----SDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRL- 180
            HLN+ALY SS +SSL MDL +RSAYDACLELLDDSV ALTRS  S+AP SS+  P  L 
Sbjct: 121 HHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLA 180

Query: 181 GSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSD 240
            SSDDVITWLSAALTNQDTCTDGFSQLSG+VKDQM DKLHNLSELVSNCLALFSGSV +D
Sbjct: 181 SSSDDVITWLSAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRND 240

Query: 241 FSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKT 300
           FSGVPIQNKRRLMNA E ED SGNFPGWMSRRERRLLTLPVGALQADIVVS++G+GTVKT
Sbjct: 241 FSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKT 300

Query: 301 IAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDN 360
           IAEAIKKAP++S R+TIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISGGKSIFDN
Sbjct: 301 IAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDN 360

Query: 361 VTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYV 420
           VTTFHTASFA +GAGIILRDMTF NWAGP RHQAVALRV  DHAVVYRCN+IGYQDTLYV
Sbjct: 361 VTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYV 420

Query: 421 HSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGIS 480
           HSNRQF+RECDIYGTVDFIFGNAAVVFQNCSIYARKAM+LQKNT+TAQNRKDPNQNTGIS
Sbjct: 421 HSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGIS 480

Query: 481 IHACRILAASDLASSN-SSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540
           IHACRILA  DLASS+ ++NPTYLGRPWKLYSRVV+M SY+G HVHPRGWLEWNATFALD
Sbjct: 481 IHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 581

BLAST of CmoCh16G000580 vs. NCBI nr
Match: XP_022922767.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata])

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180
           QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180

Query: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240
           SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF
Sbjct: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240

Query: 241 SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300
           SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI
Sbjct: 241 SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300

Query: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360
           AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV
Sbjct: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360

Query: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420
           TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH
Sbjct: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420

Query: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI 480
           SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI
Sbjct: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI 480

Query: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540
           HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL
Sbjct: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600
           YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV
Sbjct: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600

BLAST of CmoCh16G000580 vs. NCBI nr
Match: XP_023553011.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 591/600 (98.50%), Postives = 596/600 (99.33%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRR RLLLISLLSLILIV AAVSATFLVGL
Sbjct: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRRRLLLISLLSLILIVTAAVSATFLVGL 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFN+TL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNITL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180
           QHLNKALYFSS ISSLQMDLRVRSAYDAC+ELLDDSVDALTRSLQSVAPSSSNGGPQRLG
Sbjct: 121 QHLNKALYFSSEISSLQMDLRVRSAYDACVELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180

Query: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240
           SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF
Sbjct: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240

Query: 241 SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300
           SGVPIQNKRRLMNAG++ED SGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI
Sbjct: 241 SGVPIQNKRRLMNAGDDEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300

Query: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360
           AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV
Sbjct: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360

Query: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420
           TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH
Sbjct: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420

Query: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI 480
           SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPNQNTGISI
Sbjct: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISI 480

Query: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540
           HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL
Sbjct: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600
           YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV
Sbjct: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600

BLAST of CmoCh16G000580 vs. NCBI nr
Match: XP_022984620.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 590/600 (98.33%), Postives = 594/600 (99.00%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           M YGRLN+TDPGGTSSDAPS LSHHA SPNPRR RL+LISLLSLILIVAAAVSATFLVGL
Sbjct: 1   MAYGRLNQTDPGGTSSDAPSQLSHHAVSPNPRRRRLILISLLSLILIVAAAVSATFLVGL 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL
Sbjct: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180
           QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG
Sbjct: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180

Query: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240
           SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF
Sbjct: 181 SSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDF 240

Query: 241 SGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300
           SGVPIQNKRRLMNAGENED SG+FPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI
Sbjct: 241 SGVPIQNKRRLMNAGENEDTSGSFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTI 300

Query: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNV 360
           AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISGGKSIFDNV
Sbjct: 301 AEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKCNLMFVGDGKGKTVISGGKSIFDNV 360

Query: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420
           TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH
Sbjct: 361 TTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVH 420

Query: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISI 480
           SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNT+TAQNRKDPNQNTGISI
Sbjct: 421 SNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTITAQNRKDPNQNTGISI 480

Query: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540
           HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL
Sbjct: 481 HACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600
           YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV
Sbjct: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 600

BLAST of CmoCh16G000580 vs. NCBI nr
Match: XP_038881267.1 (probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 558/602 (92.69%), Postives = 575/602 (95.51%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSR--LLLISLLSLILIVAAAVSATFLV 60
           MGYGRLNE+DPGGTSS A     H  + P P RSR  L LISLLSL+LIVAAAVSATFL+
Sbjct: 1   MGYGRLNESDPGGTSSAAFQLSDHPISFPTPSRSRAQLFLISLLSLLLIVAAAVSATFLI 60

Query: 61  GLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNM 120
           G+RTAS  RSD NLPRKPTQAISKACSRTRFP+LCVNSLLDFPGSLNANEQDLVHISFN+
Sbjct: 61  GIRTASPARSDRNLPRKPTQAISKACSRTRFPSLCVNSLLDFPGSLNANEQDLVHISFNI 120

Query: 121 TLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQR 180
           TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSS+G PQR
Sbjct: 121 TLQHLNKALYLSSEISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSSGTPQR 180

Query: 181 LGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTS 240
           LGSS+DVITWLSAALTNQDTCTDGFS+LSG+VK+QMADKLHNLSELVSNCLALFSGS TS
Sbjct: 181 LGSSEDVITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETS 240

Query: 241 DFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVK 300
           DFSGVPIQNKRRLMNAG+NED SGNFPGWMSRRERRLLTLPVGALQADIVVSQNG+GTVK
Sbjct: 241 DFSGVPIQNKRRLMNAGDNEDTSGNFPGWMSRRERRLLTLPVGALQADIVVSQNGNGTVK 300

Query: 301 TIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFD 360
           TIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KSIFD
Sbjct: 301 TIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGAKSIFD 360

Query: 361 NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLY 420
           NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQDTLY
Sbjct: 361 NVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLY 420

Query: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGI 480
           VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNT+TAQNRKDPNQNTGI
Sbjct: 421 VHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQNTGI 480

Query: 481 SIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALD 540
           SIHACRILA SDLASSNSSNPTYLGRPWKLYSRVV+MLSYIGSHVHPRGWLEWNATFALD
Sbjct: 481 SIHACRILATSDLASSNSSNPTYLGRPWKLYSRVVYMLSYIGSHVHPRGWLEWNATFALD 540

Query: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600
           TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL
Sbjct: 541 TLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 600

BLAST of CmoCh16G000580 vs. NCBI nr
Match: XP_004146219.1 (probable pectinesterase/pectinesterase inhibitor 34 [Cucumis sativus] >KGN57675.1 hypothetical protein Csa_009694 [Cucumis sativus])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 546/605 (90.25%), Postives = 570/605 (94.21%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPS----HLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATF 60
           MGYGRLNETDPGG S  +P+     LS H  +PN R +RLLLIS LSL+LIVAAAVSATF
Sbjct: 1   MGYGRLNETDPGGPSHSSPASPPFQLSSHHPTPNRRTTRLLLISFLSLLLIVAAAVSATF 60

Query: 61  LVGLRTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF 120
           L+GLRTAS  RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF
Sbjct: 61  LIGLRTASPARSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISF 120

Query: 121 NMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGP 180
           N+TLQHLNKALY SS ISSLQMDLRVRSAYDACLELLDDS+DA++RSLQSVAPSSS+  P
Sbjct: 121 NVTLQHLNKALYSSSEISSLQMDLRVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTP 180

Query: 181 QRLGSSDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSV 240
           QRLGSS+D+ITWLSAALTNQDTCTDGFS+LSG+VK+QMADKLHNLSELVSNCLALFSGS 
Sbjct: 181 QRLGSSEDIITWLSAALTNQDTCTDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSE 240

Query: 241 TSDFSGVPIQNKRRLM-NAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSG 300
           TSDF+GVPIQNKRRLM   GENED+SG FP WM+RRERRLLTLPVG LQADIVVSQNG+G
Sbjct: 241 TSDFAGVPIQNKRRLMEEEGENEDSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQNGNG 300

Query: 301 TVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKS 360
           TVKTIAEAIKKAPQYS+RRTIIYVMAGRYEEKNLKVGRKK NLMFVGDGKGKTVISG KS
Sbjct: 301 TVKTIAEAIKKAPQYSSRRTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGSKS 360

Query: 361 IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQD 420
           IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVG DHAVVYRCNIIGYQD
Sbjct: 361 IFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQD 420

Query: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQN 480
           TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAM LQKNT+TAQNRKDPNQN
Sbjct: 421 TLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQN 480

Query: 481 TGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATF 540
           TGISIHACRILA SDL SSN+SNPTYLGRPWKLYSR V MLSYIG+HVHPRGWLEWNATF
Sbjct: 481 TGISIHACRILATSDLESSNTSNPTYLGRPWKLYSRTVVMLSYIGNHVHPRGWLEWNATF 540

Query: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600
           ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL
Sbjct: 541 ALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFL 600

BLAST of CmoCh16G000580 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 784.3 bits (2024), Expect = 7.2e-227
Identity = 408/611 (66.78%), Postives = 479/611 (78.40%), Query Frame = 0

Query: 1   MGYGRLNETDPGG---TSSDAPSHLSHHAASPNP----RRSRLLLISLLSLILIVAAAVS 60
           MGY RL  +   G   TS+     L+  + S  P    RRS+  L+ + S++L ++  ++
Sbjct: 1   MGYERLGPSGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLV-VSSIVLAISLILA 60

Query: 61  ATFLVGLRTASSV-RSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNA-NEQDL 120
           A    G+R+   + +S P L RKP+QAISKAC  TRFP LCV+SL+DFPGSL A + +DL
Sbjct: 61  AAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDL 120

Query: 121 VHISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSS 180
           +H++ NMTL H + ALY S+ +S + M  R RSAYD+C+ELLDDSVDAL+R+L SV  SS
Sbjct: 121 IHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSS 180

Query: 181 SNGGPQRLGSSDDVITWLSAALTNQDTCTDGFSQL-SGSVKDQMADKLHNLSELVSNCLA 240
           +   PQ      DV TWLSAALTN DTCT+GF  +  G VKD M   L NLSELVSNCLA
Sbjct: 181 AK--PQ------DVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLA 240

Query: 241 LFSGSVT-SDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVV 300
           +FS S    DF+GVPIQN RRL+   E E+    FP WM  +ER +L +PV  +QADI+V
Sbjct: 241 IFSASHDGDDFAGVPIQN-RRLLGVEEREE---KFPRWMRPKEREILEMPVSQIQADIIV 300

Query: 301 SQNGSGTVKTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTV 360
           S++G+GT KTI+EAIKKAPQ S RR IIYV AGRYEE NLKVGRKK NLMFVGDGKGKTV
Sbjct: 301 SKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTV 360

Query: 361 ISGGKSIFDNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCN 420
           ISGGKSIFDN+TTFHTASFAATGAG I RD+TFENWAGP +HQAVALR+G DHAV+YRCN
Sbjct: 361 ISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCN 420

Query: 421 IIGYQDTLYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNR 480
           IIGYQDTLYVHSNRQF+RECDIYGTVDFIFGNAAVV QNCSIYARK MD QKNT+TAQNR
Sbjct: 421 IIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNR 480

Query: 481 KDPNQNTGISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWL 540
           KDPNQNTGISIHA R+LAASDL ++N S  TYLGRPWKL+SR V+M+SYIG HVH RGWL
Sbjct: 481 KDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWL 540

Query: 541 EWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPS 600
           EWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI ST EA++FTVA+FIYGSSWLPS
Sbjct: 541 EWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPS 598

BLAST of CmoCh16G000580 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 764.6 bits (1973), Expect = 5.9e-221
Identity = 385/602 (63.95%), Postives = 464/602 (77.08%), Query Frame = 0

Query: 1   MGYGRLNETDPGGTSSDAPSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGL 60
           MGY RL  + P   +   P+      + P  ++     + L +L ++V   V      G+
Sbjct: 1   MGYDRLGPSGPSNPNQKDPA-----TSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGI 60

Query: 61  RTASSVRSDPNLPRKPTQAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTL 120
           R   S +++P L RKPTQAIS+ CS++ +P LC+++LLDFPGSL A+E +L+HISFN TL
Sbjct: 61  RAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATL 120

Query: 121 QHLNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLG 180
           Q  +KALY SS I+  QM  RVRSAYD+CLELLDDSVDALTR+L SV   S +       
Sbjct: 121 QKFSKALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGD------E 180

Query: 181 SSDDVITWLSAALTNQDTCTDGFSQL---SGSVKDQMADKLHNLSELVSNCLALFSGSVT 240
           S  DV+TWLS+A+TN DTCTDGF ++    G VKDQ+   + +LSE+VSNCLA+F+G V 
Sbjct: 181 SHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKV- 240

Query: 241 SDFSGVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 300
            D SGVP+ N R+L+   E E+     P W+ R +R LL  P  A+QADI VS++GSGT 
Sbjct: 241 KDLSGVPVVNNRKLLGTEETEE----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTF 300

Query: 301 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIF 360
           KTIAEAIKKAP++S+RR +IYV AGRYEE+NLKVGRKK NLMF+GDGKGKTVI+GGKSI 
Sbjct: 301 KTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIA 360

Query: 361 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 420
           D++TTFHTA+FAATGAG I+RDMTFEN+AGP +HQAVALRVGGDHAVVYRCNIIGYQD L
Sbjct: 361 DDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDAL 420

Query: 421 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTG 480
           YVHSNRQF+REC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK T+TAQNRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTG 480

Query: 481 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 540
           ISIHAC++LA  DL +S  S PTYLGRPWKLYSRVV+M+S +G H+ PRGWLEWN  FAL
Sbjct: 481 ISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFAL 540

Query: 541 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 600
           D+LYYGEYMN G G  +GQRV WPGY VITSTVEASKFTVAQFI GSSWLPSTGV+F  G
Sbjct: 541 DSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSG 586

BLAST of CmoCh16G000580 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 424.1 bits (1089), Expect = 1.9e-118
Identity = 243/601 (40.43%), Postives = 345/601 (57.40%), Query Frame = 0

Query: 18  APSHLSHHAASPNPRRSRLLLISLLSLILIVAAAVSATFLVGLRTASSVRSDPNLPRKPT 77
           APS     +     +  +L+L+S    +L VAA    +       AS       L     
Sbjct: 2   APSMKEIFSKDNFKKNKKLVLLSAAVALLFVAAVAGIS-----AGASKANEKRTLSPSSH 61

Query: 78  QAISKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNMTLQHLNKALYFSSGISSLQ 137
             +  +CS TR+P LC+++++   G    +++D++  S N+T+  +    +    +   +
Sbjct: 62  AVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKR 121

Query: 138 MDL--RVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLGSSDDVITWLSAALTN 197
             L  R ++A   CLE +D+++D L  +++ +    +    +    + D+ T +S+A+TN
Sbjct: 122 KGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLRE--HAGDLKTLISSAITN 181

Query: 198 QDTCTDGFS--QLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDFSGVP-----IQNK 257
           Q+TC DGFS       V+  +     ++  + SN LA+      +D +          N 
Sbjct: 182 QETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNN 241

Query: 258 RRLMN-----------AGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTV 317
           R+L             AG  E  S  +P W+S  +RRL  L    ++AD  V+ +GSGT 
Sbjct: 242 RKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTF 301

Query: 318 KTIAEAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIF 377
           KT+A A+  AP+ SN+R +I++ AG Y E N++V +KK N+MF+GDG+ +T+I+G +++ 
Sbjct: 302 KTVAAAVAAAPENSNKRYVIHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVV 361

Query: 378 DNVTTFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTL 437
           D  TTFH+A+ AA G   + RD+TF+N AGP +HQAVALRVG D +  Y C+++ YQDTL
Sbjct: 362 DGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTL 421

Query: 438 YVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTG 497
           YVHSNRQF+ +C I GTVDFIFGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTG
Sbjct: 422 YVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTG 481

Query: 498 ISIHACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFAL 557
           I I  CRI A SDL S   S PTYLGRPWK YS+ V M S I   + P GW EW  TFAL
Sbjct: 482 IVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFAL 541

Query: 558 DTLYYGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGG 599
           +TL Y EY N G G     RV W G++VIT+  EA K+T  QFI G  WL STG  F  G
Sbjct: 542 NTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLG 592

BLAST of CmoCh16G000580 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-117
Identity = 220/477 (46.12%), Postives = 296/477 (62.05%), Query Frame = 0

Query: 123 LNKALYFSSGISSLQMDLRVRSAYDACLELLDDSVDALTRSLQSVAPSSSNGGPQRLGS- 182
           + KA+   S         RV +A   C++LLD + + L+  +   A  S NG     G  
Sbjct: 58  ITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELSWIIS--ASQSPNGKDNSTGDV 117

Query: 183 SDDVITWLSAALTNQDTCTDGFSQLSGSVKDQMADKLHNLSELVSNCLALFSGSVTSDFS 242
             D+ TW+SAAL+NQDTC DGF   +G +K  +A  L  +   V N L +     +    
Sbjct: 118 GSDLRTWISAALSNQDTCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSK--- 177

Query: 243 GVPIQNKRRLMNAGENEDASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTIA 302
             P     +     +       FP W+   +R+LL        AD VV+ +G+G   TI+
Sbjct: 178 --PKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTD-NITVADAVVAADGTGNFTTIS 237

Query: 303 EAIKKAPQYSNRRTIIYVMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNVT 362
           +A+  AP YS +R +I+V  G Y E N+++ +KKWN+M VGDG   TVI+G +S  D  T
Sbjct: 238 DAVLAAPDYSTKRYVIHVKRGVYVE-NVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWT 297

Query: 363 TFHTASFAATGAGIILRDMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVHS 422
           TF +A+FA +G G I RD+TF+N AGP +HQAVA+R   D  V YRC + GYQDTLY HS
Sbjct: 298 TFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHS 357

Query: 423 NRQFYRECDIYGTVDFIFGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISIH 482
            RQF+REC I GTVDFIFG+A  VFQ+C I A++ +  QKN++TAQ RKDPN+ TG +I 
Sbjct: 358 MRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQ 417

Query: 483 ACRILAASDLASSNSSNPTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTLY 542
              I A +DL  + ++  TYLGRPWKLYSR VFM +Y+   ++P GWLEWN  FALDTLY
Sbjct: 418 FSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLY 477

Query: 543 YGEYMNYGPGGAVGQRVTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGL 599
           YGEYMN GPG ++ +RV WPGY V+ ++ EA+ FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 478 YGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of CmoCh16G000580 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 418.7 bits (1075), Expect = 7.9e-117
Identity = 237/522 (45.40%), Postives = 310/522 (59.39%), Query Frame = 0

Query: 92  LCVNSLLDFP-GSLNANEQDLVHISFNMTLQHLNKALYFSSGISSLQMDLRVRSAYDACL 151
           LCV+S  +F    L     + V  S N  +  + +     S  +    D R+++A   CL
Sbjct: 15  LCVSSEENFQYDYLKVPASEFVS-SINTIVVVIRQVSSILSQFADFSGDRRLQNAVSDCL 74

Query: 152 ELLDDSVDALTRSLQ-SVAPSSSNGGPQRLGSSDDVITWLSAALTNQDTCTDGFSQLSGS 211
           +LLD S + LT S   S  P     G   +GS  D  TWLSAAL+NQ TC +GF   SG 
Sbjct: 75  DLLDFSSEELTWSASASENPKGKGNGTGDVGS--DTRTWLSAALSNQATCMEGFDGTSGL 134

Query: 212 VKDQMADKLHNLSELVSNCLALFS-----------GSVTSDFSGVPIQNKRRLMNAGENE 271
           VK  +A  L  L  ++   L L             G +       P +  R       +E
Sbjct: 135 VKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR-----DTDE 194

Query: 272 DASGNFPGWMSRRERRLLTLPVGALQADIVVSQNGSGTVKTIAEAIKKAPQYSNRRTIIY 331
           D S  FP W+   +R+L  L       D+ V+ +G+G    I +AIKKAP YS+ R +IY
Sbjct: 195 DESLQFPDWVRPDDRKL--LESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIY 254

Query: 332 VMAGRYEEKNLKVGRKKWNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAATGAGIILR 391
           +  G Y E N+++ +KKWN++ +GDG   TVISG +S  D  TTF +A+FA +G G + R
Sbjct: 255 IKKGLYLE-NVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLAR 314

Query: 392 DMTFENWAGPGRHQAVALRVGGDHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFI 451
           D+TF+N AGP +HQAVALR   D +V +RC + GYQDTLY H+ RQFYREC I GTVDFI
Sbjct: 315 DITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFI 374

Query: 452 FGNAAVVFQNCSIYARKAMDLQKNTLTAQNRKDPNQNTGISIHACRILAASDLASSNSSN 511
           FG+  VVFQNC I A++ +  QKNT+TAQ RKD NQ +G SI    I A +DL    ++ 
Sbjct: 375 FGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTT 434

Query: 512 PTYLGRPWKLYSRVVFMLSYIGSHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRV 571
            TYLGRPWKLYSR VF+ + +   V P GWLEWNA FALDTL+YGE+MNYGPG  +  RV
Sbjct: 435 RTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRV 494

Query: 572 TWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 601
            WPGY V  ++ +A+ FTV+QFI G+ WLPSTGV F  GL +
Sbjct: 495 KWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGLYI 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3B01.0e-22566.78Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
Q9FK058.3e-22063.95Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q430629.4e-12348.92Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
P839487.5e-12042.63Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O048862.8e-11942.56Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E4870.0e+00100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430666 PE=3 SV=1[more]
A0A6J1J9300.0e+0098.33Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111482853 PE=3 SV=1[more]
A0A0A0L6S00.0e+0090.25Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G239880 PE=3 SV=1[more]
A0A5A7U3N70.0e+0090.41Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00145... [more]
A0A6J1G0F62.8e-27982.56Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449561 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022922767.10.0e+00100.00probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita moschata][more]
XP_023553011.10.0e+0098.50probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita pepo subsp. pepo][more]
XP_022984620.10.0e+0098.33probable pectinesterase/pectinesterase inhibitor 34 [Cucurbita maxima][more]
XP_038881267.10.0e+0092.69probable pectinesterase/pectinesterase inhibitor 34 [Benincasa hispida][more]
XP_004146219.10.0e+0090.25probable pectinesterase/pectinesterase inhibitor 34 [Cucumis sativus] >KGN57675.... [more]
Match NameE-valueIdentityDescription
AT3G49220.17.2e-22766.78Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.15.9e-22163.95pectin methylesterase PCR fragment F [more]
AT3G14310.11.9e-11840.43pectin methylesterase 3 [more]
AT3G43270.11.6e-11746.12Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33220.17.9e-11745.40pectin methylesterase 44 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 75..231
e-value: 4.8E-46
score: 169.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 79..231
e-value: 4.0E-33
score: 114.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 54..232
e-value: 3.5E-39
score: 131.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 280..598
e-value: 6.6E-116
score: 389.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 286..583
e-value: 2.7E-140
score: 466.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 77..234
e-value: 1.7E-36
score: 127.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 78..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..23
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 26..598
NoneNo IPR availablePANTHERPTHR31707:SF222PECTINESTERASEcoord: 26..598
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 83..233
e-value: 9.60891E-46
score: 156.83
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 431..440
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 282..596

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G000580.1CmoCh16G000580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity