CmoCh16G000200 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G000200
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRibosome-binding factor PSRP1
LocationCmo_Chr16: 109316 .. 114736 (+)
RNA-Seq ExpressionCmoCh16G000200
SyntenyCmoCh16G000200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCTCTAACCTACAAAATTGAAGTTTAAATTACATTTATTTAAGAAGATTGATGCTGCTGCAGTGGCACGGGATTCGTCCTGCGTCCACAAAAAGGCCTCGCTTTCTTTATCCATCTGTTTCCCTTCTCTCCTTGTTTCTTCAGTTTTCAAATTCCTGTTCCCATTCCCATGGCCGCTCTCACCTCTTCTATGCAAACGAATCTCCTCTCCGCCCATTCCTTAACGCCTTCTTCTTCTTCTTCTTCTTCTTCTACAGTTTCTACTTTTCCCACCTCCTTCTCTCTTCCCACCTCTACCAACTCCGCTGTTCTCAAAACTCACGCTCTCTCTTCCCATGCTTCTGCCTTCTTCAACTCCTGCAACCACTGCTTTCGGACCATTGTGGTAGCACCACGCAAACGTCATGCCACAAGATCTTTCAGTATTCGGATGTCCTGGGACGGCCCTCTCTCCTCCGTCAAATTAATTGTTCAAGGCAAAAATTTGGAGGTATTTTAGCTTCATCTTCGCTGTTGTTTCTCAGGCAATGCAAGCTTCTGTTCCAATTCTTGTTTTCAGTTTTTTTTCACGTGTTTCCTACTGCGCTGAATTGTTGGAGTCTTAGGTCTGTCCAGGACTTCTTTACTAGTGAGTGTCTCGACTAGTCGTTCTCTAATGATTTGGTTCCTTAATCGGATCGTGCTCCTTATTGGGAGTAAGATCTAGAGAGAAATCTCGTGCTCCTTATTCGTCTTCCTTAATCGACCTGCTAACTCACTACCCTTAATAATTGAAGCGTGCTTCCAAAACTATCCACTAGTTACAAACCCAATAATTTCTCCCCCCGAACTGAACATTTCAAACACTAATTAGAAACCTATTCCACTGAGTTTCTCCCTTTACTCAAAGCCTTCTCCATTACTTTTATTTGCTCCAGCTAAAACTGCTAATTTCTATGCGTCATTTCTCATGCGTTTTCCATATCAAAACTCATGTCCCTTCTCCATTTTTTACTCTACACAGCCTCTCTGAAATGATCAGTATCAACAATAGTAAGCATGGCATCAGGAATCCACTACAATTTTTTTTTTTTCTCTAAAAAGGGTCCTCACCCTATTTCCTAACCTTCATCCAAATTGCTAGTCTTTTATATCTTCGTTTATTGCCGCTAGGGGATGGTTTTTAGGTGCATTCATTTTCTTCTATATCAGCATCTAAACCAAATTTTCTTCTCATCAAACACAACCATTTCCCTTCCATGATTATTTCATTTGGGGTAACACGCAATGGCAACTTCGTATTTCCCAATTTCAATTGTTTTCTTCTTCAGATACAACATATCTGCTTGAGGGATTCCTTTGTAAAATTGGATCACGAAATCTATAGGCCCTTGGCTTTTCACTAAACCAAGCCGCACAACTCGAGCTTTATCATCCTGCTTAGTTCTTCAGCATGATTCACACATCCAAAATTAGTTAAGTACTCAACAGGCCAACTCCACTCCCATTTGGAGTTCTTATTTTAGTGGGTCCTATCTGGAGACATACTGAAAACTTCTTCCAAATCAAAAGAAACAAAAGAAAAAATGGGTTCATTTGGAAGAAAGATGGAGTCCGACCTGTAGAGCACATGTAAGGCACAGTCGGTTGATCGGATCTCTATCTCTCTCTAGATATCTAGAGATGGAGAGAGATGTGAAAGTAATAGGCTTATAAAAGGTTTGGAACTCGAATTCCACTTGACGAGGGTATGGGATTGCTTGACTACATGCAAAAACTTTTCCTTCGACTGGATTGAATAATGATCTTTATTTCCAAGCTTAAGCTTAATGCTTGCGAGGCCAAGGTGGAACTGATTAAGCTTAAGCTTGGTTGATTTTGTCAAGGTGAAGAACTGGTTAAGCTTAAGCTTGGTTGATTTTGTCAAGGTGAAAGAACTGATTAAGCTTAGTTGATTTTGTCAGAAGAAAGTAGGTTACGCTGGTTCATTGATTAGTAAACCTCCCATGCTGGTAAAGCTTAGCTAAAGCCATACCCGCGAGATCCCATAGTGACTTGGCTTTGGGGTGGTTCTCCGTGGACAATGCCACCTCAAATCATTTTTCTTTTTCTCTTGCATCAAGTTGACCCACTCCCCACTATGTAGTGATTATAATCGATCATGTACGTGTCATCCCCTCCATTCCGATTGGTATATCATGAAAAAATCAAGCCCTTTGCACTACTGCTTTCTCTTGCCCAATGTTAGCCTTGTCAAAAGGTAGGTTTCCGCTCGTTGGTTAATTCATCCTGCAAGTCTATTTCTTTGAATTAGTACTGGTTTCTAGGAAAGTTGTTAATGATATTTTTAGTCAGTTAGCTTAAGTTTCTATACTTGGAGATACCGGCTAGAGTTGGGTAAGTGTAGTTTGTTGCTGATTTTATGGAGTTAACTAGGCTATTTTTAGCATTTGGAATTTGTATTTATAGTGACAAGTGATTGAATAAAACTGTTCACTTCCCAATTCTTCTCTATCTATTAAGAACAGCACCACTGTGAACAGCCTAATTTGCTGGTTCTGGCCAGAAGAAATTTGGGCGTTCTTTAAGCATAGTAATAGTCATTAAGATTATGTTTTTTAAAGCATGCGAATCTCCAAATTGGTGGTCATATCATTCTTTTATACAACTACATTTAGTTATCGTGTTTATGCAACCGTCAGTTGTTGAATGCTATTTGAACTCAAAAATTGGTGAACGCATGTGAAACTCTTTTCAATCAGTACATAGGGGCATATGGCCTTTCCTACTGATCAAAACAAGTTTGACAAGTCCACACTTTAGAGTGTACTCTTTGAAGCATATGTGAAACTCTCTAGATCCTTTCACGCCATTGATTTCCTTTGTCCATTACGAAGTAAACATTGCATAAATTTGGAAGAGACTTAAGCTAAGCTCTAGCTATGTTGAAGTTTCATTAAGCCCTTCAACTCAGCTATCCATGTCTTCAATGCTAAAGCTGCACAATATCTTCACCCGTAGATACTATAGGTTAAGGTATAACTTTGAGCATGAACTTATTCACCGCCATTATTAGACTCTTCCAAGATGGAAATAATTTGCACTTTCTTTCCTAAAATAGGAGACAGAGAATTTAAAATTTGCATTCAAAATCTATATGAAAGTAGTTTAAAGATAATATCTGTAGAGTCAAATCAAAAGGGCTAGCTTCAAATTCATTGAAGAGAAGGTTTAGAAACTAACCATTGACTGTAGGTCATCTTCTCCAACTTGCGTTCGTGCCATTCTGCTTCTTTTTTTATTTGTTTTCTTAAAATTCCCGGTGTTACAATAGCACTACTCTGATCGGCAAATTATTATTCAGCCCTTTACAGCACTACTAACACGTGTAAAAAAATTACCTTGTTGGTAGAAACTCCAATGAGAATGACAAGACCACCAACTAGCTATCGGAATTTCAATTACCCAATTTATCATATCTCAATGTTTCAAATGTCGGACCTTGAGTGGAGTTATTTTTTTTACTGTGCAAAATACTAGTTGACTGAGGCAGTTAAGAAGCACGTGGAAGAGAAGGTTGGCAAGGCAGTCCAGAAGCACAGTCACTTAGTGAGAGAAGTAGATGTCAGACTATCAGTTAGAGGTGGTGAGTTTGGGAAGGGCCCAAGAATTAGAAGATGCGAGGTATTGTATTGTTCCTTTATTTTAAATTTATCCATATGAATTGATTCTTTTTCTTTTCTAAAAACATGTGTGTTTGTGTAGGTTACTTTATTCACGAAGAAGCATGGAGTTGTACGAGCAGAGGAAGATGCCGAGACCGTGTATGCAAGCATTGATTTGGTATCTTCCATCATGCAGAGAAAGCTGAGAAAGATTAAAGAGAAAGATTCAGATCATGGCCGGCACATGAAAGGATTTGACAGATCGAAAATAAGAGAACCTGCGCCAATAGTAGTTGAAGATGAGGAAGAAGAGGTTTCTCAGGAAGATGGTGGAGAAGCTATTGATGAGGTTATTCCTTCAACCTTTGGTTTTTTTTTTTTTACCTTGAGTTCATTTTGCTGCATATATTAATCATCTAAGGTGATATTAGCTTGTACAAACTCATGGGTTCCATATTTACTATTTTCCACATGTTGAAGCTAATATGAGTGCTAGAGTTACTAAACCACAACTGTTATTGCTTAGTTAGAAATTGTTGTTTTCTCTAGTTTCTTATAAGGAAAAACAATCATCGATTTCGTAGTTGATTTTCTTTTTCCTATCTAGTGGTTGGTCTGAGGAAATGCTAGATGCAGAACTCAGCATCAGTTGAACCAGGTGCAATAAGTTACTCCTTGTGTAGGGGGGAAGAATGGAAAGCAGTGCATTCTCAATCTTGATTTCCCCTTCTGCTGTGCTTTTTTCAACTAAATTCTGCCCAACTCATCGTAGTTATATTTACTGTGAGATCTCACATCAATTGGGAAGGAAACGAAGCATTCTTTATAAGATTGTGGAAACCTTTCCCTAGCAAACATGTTTTAATAACCTCGAGGGGAAACCCAAAAGAAAAAGTTCAAAAAGAACAATATCTGCTAGCGGTATAAGGTTAATCAGTCAGGTCTTTTCTTTCACAACATGCTATTTTTGGTGAACATGAAAGCATCGGAAATTGGATCTTGTAGATGTAAGATAGAGGGCCTCCATAGTTGTAGAAGCCTAATATTTGTTATTTCTTGCGGAGCCAGCGTTGTGCTCAACTGTTATTGATTAGCTTCTAACTGTCGGACAGTACTTCATTGTCTACAAGGGACTGTTACATTAGAATTTCCTTTCAACTTTATCCCTTTAACCTGCTTAGAGTTACCAAGTTGGTTCATCACAGCAATTGAGACATCCTTCAAAGCTTGCATAGACCCTTTGCTCTTAACGAGTGTTTTATGGCCCCTTCCAACGTACTTGATCGTTATAGCTTTTGATTCATTTGCCTATTTTGTTGACATTCACCCCTAACCTGGGTATCATTTTTAGATGACTAAATCATGCATCATATATTGGAAGCTTGTGAATAATATATCGAAATGGTTAAAATGTTGTTTTTCCTTTTTCATGTTTATCTTGATTTCAGATTGTTCGGACAAAATACTTCGATATGCCGCCGTTGACAGTTGAAGAAGCAATCGAACAACTAAAGAATATTGACCATGATTTCTATGGTTTCCGGAATGAAGAAACTGGTGCGTTGATTTGATTATTAAAAAAAAAAAACTTGCTTTGTTGGGATTCTGTGCTGTTTATATCGTGGCTTGTTTCATCTTTGGCTCAGGTGAGATTAACATCTTATATAAAAGAAAGGATGGAGGATATGGACTTATCATCCCAAAAGAAGATGGCAATGCTGAGAAAATAGAAACTGTGACGGTTGAAGTAGCCAGAGAAGCCTCCTTGGCGGAATGA

mRNA sequence

TCCTCTAACCTACAAAATTGAAGTTTAAATTACATTTATTTAAGAAGATTGATGCTGCTGCAGTGGCACGGGATTCGTCCTGCGTCCACAAAAAGGCCTCGCTTTCTTTATCCATCTGTTTCCCTTCTCTCCTTGTTTCTTCAGTTTTCAAATTCCTGTTCCCATTCCCATGGCCGCTCTCACCTCTTCTATGCAAACGAATCTCCTCTCCGCCCATTCCTTAACGCCTTCTTCTTCTTCTTCTTCTTCTTCTACAGTTTCTACTTTTCCCACCTCCTTCTCTCTTCCCACCTCTACCAACTCCGCTGTTCTCAAAACTCACGCTCTCTCTTCCCATGCTTCTGCCTTCTTCAACTCCTGCAACCACTGCTTTCGGACCATTGTGGTAGCACCACGCAAACGTCATGCCACAAGATCTTTCAGTATTCGGATGTCCTGGGACGGCCCTCTCTCCTCCGTCAAATTAATTGTTCAAGGCAAAAATTTGGAGTTGACTGAGGCAGTTAAGAAGCACGTGGAAGAGAAGGTTGGCAAGGCAGTCCAGAAGCACAGTCACTTAGTGAGAGAAGTAGATGTCAGACTATCAGTTAGAGGTGGTGAGTTTGGGAAGGGCCCAAGAATTAGAAGATGCGAGGTTACTTTATTCACGAAGAAGCATGGAGTTGTACGAGCAGAGGAAGATGCCGAGACCGTGTATGCAAGCATTGATTTGGTATCTTCCATCATGCAGAGAAAGCTGAGAAAGATTAAAGAGAAAGATTCAGATCATGGCCGGCACATGAAAGGATTTGACAGATCGAAAATAAGAGAACCTGCGCCAATAGTAGTTGAAGATGAGGAAGAAGAGGTTTCTCAGGAAGATGGTGGAGAAGCTATTGATGAGATTGTTCGGACAAAATACTTCGATATGCCGCCGTTGACAGTTGAAGAAGCAATCGAACAACTAAAGAATATTGACCATGATTTCTATGGTTTCCGGAATGAAGAAACTGGTGAGATTAACATCTTATATAAAAGAAAGGATGGAGGATATGGACTTATCATCCCAAAAGAAGATGGCAATGCTGAGAAAATAGAAACTGTGACGGTTGAAGTAGCCAGAGAAGCCTCCTTGGCGGAATGA

Coding sequence (CDS)

ATGGCCGCTCTCACCTCTTCTATGCAAACGAATCTCCTCTCCGCCCATTCCTTAACGCCTTCTTCTTCTTCTTCTTCTTCTTCTACAGTTTCTACTTTTCCCACCTCCTTCTCTCTTCCCACCTCTACCAACTCCGCTGTTCTCAAAACTCACGCTCTCTCTTCCCATGCTTCTGCCTTCTTCAACTCCTGCAACCACTGCTTTCGGACCATTGTGGTAGCACCACGCAAACGTCATGCCACAAGATCTTTCAGTATTCGGATGTCCTGGGACGGCCCTCTCTCCTCCGTCAAATTAATTGTTCAAGGCAAAAATTTGGAGTTGACTGAGGCAGTTAAGAAGCACGTGGAAGAGAAGGTTGGCAAGGCAGTCCAGAAGCACAGTCACTTAGTGAGAGAAGTAGATGTCAGACTATCAGTTAGAGGTGGTGAGTTTGGGAAGGGCCCAAGAATTAGAAGATGCGAGGTTACTTTATTCACGAAGAAGCATGGAGTTGTACGAGCAGAGGAAGATGCCGAGACCGTGTATGCAAGCATTGATTTGGTATCTTCCATCATGCAGAGAAAGCTGAGAAAGATTAAAGAGAAAGATTCAGATCATGGCCGGCACATGAAAGGATTTGACAGATCGAAAATAAGAGAACCTGCGCCAATAGTAGTTGAAGATGAGGAAGAAGAGGTTTCTCAGGAAGATGGTGGAGAAGCTATTGATGAGATTGTTCGGACAAAATACTTCGATATGCCGCCGTTGACAGTTGAAGAAGCAATCGAACAACTAAAGAATATTGACCATGATTTCTATGGTTTCCGGAATGAAGAAACTGGTGAGATTAACATCTTATATAAAAGAAAGGATGGAGGATATGGACTTATCATCCCAAAAGAAGATGGCAATGCTGAGAAAATAGAAACTGTGACGGTTGAAGTAGCCAGAGAAGCCTCCTTGGCGGAATGA

Protein sequence

MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAFFNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAEKIETVTVEVAREASLAE
Homology
BLAST of CmoCh16G000200 vs. ExPASy Swiss-Prot
Match: P19954 (Ribosome-binding factor PSRP1, chloroplastic OS=Spinacia oleracea OX=3562 GN=PSRP1 PE=1 SV=2)

HSP 1 Score: 330.9 bits (847), Expect = 1.6e-89
Identity = 184/322 (57.14%), Postives = 240/322 (74.53%), Query Frame = 0

Query: 8   MQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAFFNSC--- 67
           M T   SA ++ P+ ++S S++++   T    PT+          LSS  S F NSC   
Sbjct: 1   MATLCTSAINMNPNLTNSLSNSINLSST----PTN----------LSSLRSTFTNSCSLG 60

Query: 68  -NHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKA 127
            N   +++ ++  K +      + MSWDGPLSSVKLI+QG+NLE+++ V+ HVE+KVGK+
Sbjct: 61  LNVAVKSVQISRNKPNV-----VCMSWDGPLSSVKLILQGRNLEVSDNVRSHVEDKVGKS 120

Query: 128 VQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVS 187
           V KHSHLVREVDVRLS RGG+  KGP++RRCEVTLFTK+HGV+RAEEDAE++Y+SIDLVS
Sbjct: 121 VAKHSHLVREVDVRLSARGGDLSKGPKLRRCEVTLFTKRHGVIRAEEDAESLYSSIDLVS 180

Query: 188 SIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEE--EEVSQE------DGGEA 247
           SI+QRKLRKIK+K SDHGRHMKGF+RSK+R+P P+ +  EE  EEV         +  + 
Sbjct: 181 SIIQRKLRKIKDKVSDHGRHMKGFNRSKVRDPEPVRITREEVLEEVESAPAPVSVEDDDF 240

Query: 248 IDEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKE 307
           I+E+VRTKYFDMPPLT+ EA+EQL+N+DHDFY FRNEETG+INILYKRK+GGYGLIIPK 
Sbjct: 241 IEEVVRTKYFDMPPLTITEAVEQLENVDHDFYAFRNEETGDINILYKRKEGGYGLIIPK- 300

Query: 308 DGNAEKIETVTVEVAREASLAE 318
           DG  EK+E++ V+  ++ S AE
Sbjct: 301 DGKTEKLESLPVQTDKQPSFAE 302

BLAST of CmoCh16G000200 vs. ExPASy Swiss-Prot
Match: P74518 (Ribosome hibernation promotion factor OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=hpf PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 88/204 (43.14%), Postives = 129/204 (63.24%), Query Frame = 0

Query: 97  VKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRI---RR 156
           +KL++QG N+ +TEA+  +VEEKV +AV+   +L  +VDV LSV      +  RI    +
Sbjct: 1   MKLLIQGNNITVTEAIHDYVEEKVERAVKHFQNLTTKVDVHLSV-----ARNARITNKHK 60

Query: 157 CEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEK--DSDHGRHMKGFDRSK 216
            EVT++     V+RA+E +E +YASIDLV+  + R+LRK KE+  D  HG        S+
Sbjct: 61  AEVTVYA-NGTVIRAQEGSENLYASIDLVADKIARQLRKYKERIQDKQHG----NVKTSE 120

Query: 217 IREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRN 276
           I E  P+     EE +  +   E   E++R KYF MPP+ +E+A+EQL+ +DHDFY FRN
Sbjct: 121 IVEDKPV-----EENLIGDRAPELPSEVLRMKYFAMPPMAIEDALEQLQLVDHDFYMFRN 180

Query: 277 EETGEINILYKRKDGGYGLIIPKE 296
           ++T EIN++Y R  GGYG+I P +
Sbjct: 181 KDTDEINVIYIRNHGGYGVIQPHQ 189

BLAST of CmoCh16G000200 vs. ExPASy Swiss-Prot
Match: P47908 (Ribosome hibernation promotion factor OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=hpf PE=2 SV=2)

HSP 1 Score: 134.4 bits (337), Expect = 2.2e-30
Identity = 78/199 (39.20%), Postives = 122/199 (61.31%), Query Frame = 0

Query: 97  VKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEV 156
           +KL++QG N+ +TE++  +VE K+ KA +       +VDV LSV      +     + EV
Sbjct: 1   MKLLIQGNNIAVTESIHDYVESKLEKATKHFQTFATKVDVHLSVANN--ARITDKHKAEV 60

Query: 157 TLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPA 216
           T++     V+RA+E +E +YASIDLVS  + R+LRK KEK+     H++        + +
Sbjct: 61  TVYA-NGTVIRAQEGSENLYASIDLVSDKIARQLRKYKEKNFGKKTHVQ-------EKTS 120

Query: 217 PIVVEDE-EEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETG 276
            ++ ED   + +  +   E   E+VR KYF MPP+T++EA+EQL+ +DHDFY F N++T 
Sbjct: 121 EVLPEDPVPDNLIGDRAPELPSEVVRMKYFAMPPMTIDEALEQLQLVDHDFYMFLNKDTN 180

Query: 277 EINILYKRKDGGYGLIIPK 295
            IN++Y R  GGYG+I P+
Sbjct: 181 AINVIYIRNHGGYGVIQPR 189

BLAST of CmoCh16G000200 vs. ExPASy Swiss-Prot
Match: Q9RVE7 (Ribosome hibernation promotion factor OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=hpf PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.7e-20
Identity = 69/198 (34.85%), Postives = 117/198 (59.09%), Query Frame = 0

Query: 101 VQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFT 160
           + G+N+E+TE ++++VEEK+ + + +++  + +  V L+VR  +     R  R EV L  
Sbjct: 6   LSGRNVEVTEPMREYVEEKLSR-LDRYTDQITDARVTLTVR--DVRNNERRNRVEVQL-N 65

Query: 161 KKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVV 220
              G++RAEE    +YA+ID  S +++R+LRK K       R+MK     +  EP P   
Sbjct: 66  VPGGIIRAEEHHADMYAAIDKASDVLERQLRKFKT------RYMKQRQEGR-PEPLP--- 125

Query: 221 EDEEEEVSQEDGGEAID-------EIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEE 280
              E EV+ +  G A+D       EIVR K F++ P++ E+A+ Q++ + HDFY F++ +
Sbjct: 126 GPAEAEVNAQGSGAAMDDVSEFHPEIVRQKRFELRPMSAEDAVVQMEALGHDFYVFQDLQ 185

Query: 281 TGEINILYKRKDGGYGLI 292
            G+  ++Y+R+DG YGLI
Sbjct: 186 -GQTGVVYRRRDGHYGLI 188

BLAST of CmoCh16G000200 vs. ExPASy Swiss-Prot
Match: Q4L4H7 (Ribosome hibernation promotion factor OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=hpf PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 4.6e-20
Identity = 67/202 (33.17%), Postives = 116/202 (57.43%), Query Frame = 0

Query: 97  VKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEV 156
           ++  + G NL +T+A++ ++E+K+GK ++++ + V      + V+  +        + EV
Sbjct: 2   IRFEIHGDNLTITDAIRNYIEDKIGK-LERYFNDVPNAVAHVKVKTYQ----NSTTKIEV 61

Query: 157 TLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEK-DSDHGRHMKGFDRSKIREP 216
           T+   K+  +RAEE  + +YA IDLV+S ++R++RK K + +  H  H          EP
Sbjct: 62  TI-PLKNVTLRAEERHDDLYAGIDLVTSKLERQVRKYKTRVNRKHKTH---------GEP 121

Query: 217 APIVVEDEE------EEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGF 276
              V E +E      ++V+ E   ++  EI+R+K F + P+  EEA+ Q++ + HDFY F
Sbjct: 122 EAFVAEVQEAPPENVDDVNAEPTNDSEIEIIRSKQFSLKPMDSEEAVLQMELLGHDFYIF 181

Query: 277 RNEETGEINILYKRKDGGYGLI 292
            + ET   +I+YKRKDG YGLI
Sbjct: 182 TDRETDGTSIVYKRKDGKYGLI 188

BLAST of CmoCh16G000200 vs. ExPASy TrEMBL
Match: A0A6J1E3S3 (ribosome-binding factor PSRP1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430564 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.3e-168
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60
           MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF
Sbjct: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60

Query: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120
           FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV
Sbjct: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120

Query: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180
           GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID
Sbjct: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180

Query: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240
           LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV
Sbjct: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240

Query: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300
           RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE
Sbjct: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300

Query: 301 KIETVTVEVAREASLAE 318
           KIETVTVEVAREASLAE
Sbjct: 301 KIETVTVEVAREASLAE 317

BLAST of CmoCh16G000200 vs. ExPASy TrEMBL
Match: A0A6J1J523 (ribosome-binding factor PSRP1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111482691 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.2e-157
Identity = 294/297 (98.99%), Postives = 296/297 (99.66%), Query Frame = 0

Query: 21  SSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAFFNSCNHCFRTIVVAPRKRHA 80
           SSSSSSSSTVSTFPTSFSLPTSTNSAVLK HALSSHASAFFNSCNHCFRTIV+APRKRHA
Sbjct: 27  SSSSSSSSTVSTFPTSFSLPTSTNSAVLKIHALSSHASAFFNSCNHCFRTIVIAPRKRHA 86

Query: 81  TRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSV 140
           TRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSV
Sbjct: 87  TRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSV 146

Query: 141 RGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDH 200
           RGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDH
Sbjct: 147 RGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDH 206

Query: 201 GRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLK 260
           GRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLK
Sbjct: 207 GRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLK 266

Query: 261 NIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAEKIETVTVEVAREASLAE 318
           NIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKE+GNAEKIETVTVEVAREASLAE
Sbjct: 267 NIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKENGNAEKIETVTVEVAREASLAE 323

BLAST of CmoCh16G000200 vs. ExPASy TrEMBL
Match: A0A6J1CJG5 (ribosome-binding factor PSRP1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111011561 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.8e-139
Identity = 275/319 (86.21%), Postives = 282/319 (88.40%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60
           MA LTSSM TNLLSA         SSSST STFP SFSL TSTNS+ LK H LSSH S F
Sbjct: 1   MATLTSSMHTNLLSA--------PSSSSTFSTFPASFSLLTSTNSSALKIHPLSSHTSPF 60

Query: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120
           FNSCN CF TI +APR RHA  SF+IRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV
Sbjct: 61  FNSCN-CFTTIQIAPRNRHAANSFTIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120

Query: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180
           GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVR EEDAETVYASID
Sbjct: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRGEEDAETVYASID 180

Query: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVED--EEEEVSQEDGGEAIDE 240
           LVSSI+QRKLRKIKEKDSD GRHMKGFDR K+REPAPIVVED  EEEEVS ED GE IDE
Sbjct: 181 LVSSIIQRKLRKIKEKDSDRGRHMKGFDRLKVREPAPIVVEDEEEEEEVSLEDSGEVIDE 240

Query: 241 IVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGN 300
           IVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKE+G 
Sbjct: 241 IVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKENGR 300

Query: 301 AEKIETVTVEVAREASLAE 318
           AEK+E VTVE ARE SLAE
Sbjct: 301 AEKLEPVTVEPAREPSLAE 310

BLAST of CmoCh16G000200 vs. ExPASy TrEMBL
Match: A0A5D3BQR1 (Ribosome-binding factor PSRP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00050 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.9e-135
Identity = 269/317 (84.86%), Postives = 281/317 (88.64%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60
           MA LTSSMQ NLLS HSL P     SSSTVS FP  FSL    + + LK HALSS+ASAF
Sbjct: 1   MATLTSSMQANLLSDHSLPP--PHPSSSTVSYFPAPFSL----HFSALKVHALSSNASAF 60

Query: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120
           FNSC + F TI  A R RHA+ SFSIRM+WDG LSSVKLIVQGKNLELTEAVKKHVEEKV
Sbjct: 61  FNSCKN-FGTIETASRNRHASNSFSIRMTWDGHLSSVKLIVQGKNLELTEAVKKHVEEKV 120

Query: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180
           GKAVQKHSHLVREVDVRLSVRGGEFGKG RIRRCEVTLFTKKHGVVRAEEDAETV+ASID
Sbjct: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGQRIRRCEVTLFTKKHGVVRAEEDAETVFASID 180

Query: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240
           LVSSI+QRKLRKIKEKDSD GRH+KGFDRSK+REPAPIVVEDEEEEV QEDGGE IDEIV
Sbjct: 181 LVSSIIQRKLRKIKEKDSDRGRHVKGFDRSKVREPAPIVVEDEEEEVFQEDGGEVIDEIV 240

Query: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300
           RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKE+G AE
Sbjct: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKENGTAE 300

Query: 301 KIETVTVEVAREASLAE 318
           K+E VTVE ARE SLAE
Sbjct: 301 KLEPVTVEAAREPSLAE 310

BLAST of CmoCh16G000200 vs. ExPASy TrEMBL
Match: A0A1S3CCT7 (ribosome-binding factor PSRP1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499579 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.9e-135
Identity = 269/317 (84.86%), Postives = 281/317 (88.64%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60
           MA LTSSMQ NLLS HSL P     SSSTVS FP  FSL    + + LK HALSS+ASAF
Sbjct: 1   MATLTSSMQANLLSDHSLPP--PHPSSSTVSYFPAPFSL----HFSALKVHALSSNASAF 60

Query: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120
           FNSC + F TI  A R RHA+ SFSIRM+WDG LSSVKLIVQGKNLELTEAVKKHVEEKV
Sbjct: 61  FNSCKN-FGTIETASRNRHASNSFSIRMTWDGHLSSVKLIVQGKNLELTEAVKKHVEEKV 120

Query: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180
           GKAVQKHSHLVREVDVRLSVRGGEFGKG RIRRCEVTLFTKKHGVVRAEEDAETV+ASID
Sbjct: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGQRIRRCEVTLFTKKHGVVRAEEDAETVFASID 180

Query: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240
           LVSSI+QRKLRKIKEKDSD GRH+KGFDRSK+REPAPIVVEDEEEEV QEDGGE IDEIV
Sbjct: 181 LVSSIIQRKLRKIKEKDSDRGRHVKGFDRSKVREPAPIVVEDEEEEVFQEDGGEVIDEIV 240

Query: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300
           RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKE+G AE
Sbjct: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKENGTAE 300

Query: 301 KIETVTVEVAREASLAE 318
           K+E VTVE ARE SLAE
Sbjct: 301 KLEPVTVEAAREPSLAE 310

BLAST of CmoCh16G000200 vs. NCBI nr
Match: XP_022922607.1 (ribosome-binding factor PSRP1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60
           MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF
Sbjct: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60

Query: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120
           FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV
Sbjct: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120

Query: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180
           GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID
Sbjct: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180

Query: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240
           LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV
Sbjct: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240

Query: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300
           RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE
Sbjct: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300

Query: 301 KIETVTVEVAREASLAE 318
           KIETVTVEVAREASLAE
Sbjct: 301 KIETVTVEVAREASLAE 317

BLAST of CmoCh16G000200 vs. NCBI nr
Match: KAG7014676.1 (Ribosome-binding factor PSRP1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 316/321 (98.44%), Postives = 317/321 (98.75%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTP----SSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSH 60
           MAALTSSMQTNLLSAHSLTP    SSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSH
Sbjct: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSH 60

Query: 61  ASAFFNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHV 120
           ASAFFNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHV
Sbjct: 61  ASAFFNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHV 120

Query: 121 EEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVY 180
           EEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVY
Sbjct: 121 EEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVY 180

Query: 181 ASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAI 240
           ASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIR+PAPIVVEDEEEEVSQEDGGEAI
Sbjct: 181 ASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIRDPAPIVVEDEEEEVSQEDGGEAI 240

Query: 241 DEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKED 300
           DEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKED
Sbjct: 241 DEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKED 300

Query: 301 GNAEKIETVTVEVAREASLAE 318
           GNAEKIETVTVEVAREASLAE
Sbjct: 301 GNAEKIETVTVEVAREASLAE 321

BLAST of CmoCh16G000200 vs. NCBI nr
Match: XP_023551717.1 (ribosome-binding factor PSRP1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589.3 bits (1518), Expect = 1.9e-164
Identity = 312/317 (98.42%), Postives = 314/317 (99.05%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTPSSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAF 60
           MAALTSSMQTNLLSAHSLTP  SSSSS+TVSTFPTSFSLPTSTNSAVLK HALSSHASAF
Sbjct: 1   MAALTSSMQTNLLSAHSLTP--SSSSSTTVSTFPTSFSLPTSTNSAVLKIHALSSHASAF 60

Query: 61  FNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120
           FNSCNHCFRTIV+APRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV
Sbjct: 61  FNSCNHCFRTIVIAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKV 120

Query: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180
           GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID
Sbjct: 121 GKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASID 180

Query: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240
           LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV
Sbjct: 181 LVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIV 240

Query: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300
           RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE
Sbjct: 241 RTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAE 300

Query: 301 KIETVTVEVAREASLAE 318
           KIETVTVEVAREASLAE
Sbjct: 301 KIETVTVEVAREASLAE 315

BLAST of CmoCh16G000200 vs. NCBI nr
Match: KAG6576623.1 (Ribosome-binding factor PSRP1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.8 bits (1514), Expect = 5.6e-164
Identity = 314/321 (97.82%), Postives = 314/321 (97.82%), Query Frame = 0

Query: 1   MAALTSSMQTNLLSAHSLTP----SSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSH 60
           MAALTSSMQTNLLS HSLTP    SSSSSSSSTVSTFPTSF LPTSTNSAVLKTHALSSH
Sbjct: 1   MAALTSSMQTNLLSTHSLTPSSSSSSSSSSSSTVSTFPTSFFLPTSTNSAVLKTHALSSH 60

Query: 61  ASAFFNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHV 120
           ASAFFNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHV
Sbjct: 61  ASAFFNSCNHCFRTIVVAPRKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHV 120

Query: 121 EEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVY 180
           EEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVY
Sbjct: 121 EEKVGKAVQKHSHLVREVDVRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVY 180

Query: 181 ASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAI 240
           ASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAI
Sbjct: 181 ASIDLVSSIMQRKLRKIKEKDSDHGRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAI 240

Query: 241 DEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKED 300
           DEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKED
Sbjct: 241 DEIVRTKYFDMPPLTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKED 300

Query: 301 GNAEKIETVTVEVAREASLAE 318
           GNAEKIETVTV VAREASLAE
Sbjct: 301 GNAEKIETVTVGVAREASLAE 321

BLAST of CmoCh16G000200 vs. NCBI nr
Match: XP_022984371.1 (ribosome-binding factor PSRP1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 563.9 bits (1452), Expect = 8.6e-157
Identity = 294/297 (98.99%), Postives = 296/297 (99.66%), Query Frame = 0

Query: 21  SSSSSSSSTVSTFPTSFSLPTSTNSAVLKTHALSSHASAFFNSCNHCFRTIVVAPRKRHA 80
           SSSSSSSSTVSTFPTSFSLPTSTNSAVLK HALSSHASAFFNSCNHCFRTIV+APRKRHA
Sbjct: 27  SSSSSSSSTVSTFPTSFSLPTSTNSAVLKIHALSSHASAFFNSCNHCFRTIVIAPRKRHA 86

Query: 81  TRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSV 140
           TRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSV
Sbjct: 87  TRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVDVRLSV 146

Query: 141 RGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDH 200
           RGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDH
Sbjct: 147 RGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKEKDSDH 206

Query: 201 GRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLK 260
           GRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLK
Sbjct: 207 GRHMKGFDRSKIREPAPIVVEDEEEEVSQEDGGEAIDEIVRTKYFDMPPLTVEEAIEQLK 266

Query: 261 NIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAEKIETVTVEVAREASLAE 318
           NIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKE+GNAEKIETVTVEVAREASLAE
Sbjct: 267 NIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKENGNAEKIETVTVEVAREASLAE 323

BLAST of CmoCh16G000200 vs. TAIR 10
Match: AT5G24490.1 (30S ribosomal protein, putative )

HSP 1 Score: 355.9 bits (912), Expect = 3.3e-98
Identity = 181/248 (72.98%), Postives = 209/248 (84.27%), Query Frame = 0

Query: 76  RKRHATRSFSIRMSWDGPLSSVKLIVQGKNLELTEAVKKHVEEKVGKAVQKHSHLVREVD 135
           R  +   S +++MSWDGPL+SVKLI+QGKNLEL+E +K+HVEEKVGK+VQKHSHLVREVD
Sbjct: 61  RHSYCRSSLAVKMSWDGPLASVKLIIQGKNLELSEPIKQHVEEKVGKSVQKHSHLVREVD 120

Query: 136 VRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYASIDLVSSIMQRKLRKIKE 195
           VRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYA IDLVS+I+QRKLRKIKE
Sbjct: 121 VRLSVRGGEFGKGPRIRRCEVTLFTKKHGVVRAEEDAETVYACIDLVSTIIQRKLRKIKE 180

Query: 196 KDSDHGRHMKGFDRSKIREPA-PIVVEDEEEEV-----SQEDGGEAIDEIVRTKYFDMPP 255
           KDSDHGRHMKGF+R K+REP    VVED E+        +E+  + I EIVRTK F+MPP
Sbjct: 181 KDSDHGRHMKGFNRLKVREPVIEPVVEDVEDSTDSSVGEEEEEDDLIKEIVRTKTFEMPP 240

Query: 256 LTVEEAIEQLKNIDHDFYGFRNEETGEINILYKRKDGGYGLIIPKEDGNAEKIETVTVEV 315
           LTV EA+EQL+ + HDFYGF+NEETGEINI+YKRK+GGYGLIIPK+DG AEK+E +  E 
Sbjct: 241 LTVAEAVEQLELVSHDFYGFQNEETGEINIVYKRKEGGYGLIIPKKDGKAEKVEPLPTEQ 300

Query: 316 AREASLAE 318
             E S AE
Sbjct: 301 LNEHSFAE 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P199541.6e-8957.14Ribosome-binding factor PSRP1, chloroplastic OS=Spinacia oleracea OX=3562 GN=PSR... [more]
P745181.5e-3443.14Ribosome hibernation promotion factor OS=Synechocystis sp. (strain PCC 6803 / Ka... [more]
P479082.2e-3039.20Ribosome hibernation promotion factor OS=Synechococcus sp. (strain ATCC 27264 / ... [more]
Q9RVE72.7e-2034.85Ribosome hibernation promotion factor OS=Deinococcus radiodurans (strain ATCC 13... [more]
Q4L4H74.6e-2033.17Ribosome hibernation promotion factor OS=Staphylococcus haemolyticus (strain JCS... [more]
Match NameE-valueIdentityDescription
A0A6J1E3S35.3e-168100.00ribosome-binding factor PSRP1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1J5234.2e-15798.99ribosome-binding factor PSRP1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1CJG51.8e-13986.21ribosome-binding factor PSRP1, chloroplastic OS=Momordica charantia OX=3673 GN=L... [more]
A0A5D3BQR15.9e-13584.86Ribosome-binding factor PSRP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CCT75.9e-13584.86ribosome-binding factor PSRP1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10349... [more]
Match NameE-valueIdentityDescription
XP_022922607.11.1e-167100.00ribosome-binding factor PSRP1, chloroplastic [Cucurbita moschata][more]
KAG7014676.11.3e-16598.44Ribosome-binding factor PSRP1, chloroplastic, partial [Cucurbita argyrosperma su... [more]
XP_023551717.11.9e-16498.42ribosome-binding factor PSRP1, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG6576623.15.6e-16497.82Ribosome-binding factor PSRP1, chloroplastic, partial [Cucurbita argyrosperma su... [more]
XP_022984371.18.6e-15798.99ribosome-binding factor PSRP1, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G24490.13.3e-9872.9830S ribosomal protein, putative [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003489Ribosome hibernation promoting factor/RaiATIGRFAMTIGR00741TIGR00741coord: 98..201
e-value: 7.4E-19
score: 66.1
IPR003489Ribosome hibernation promoting factor/RaiAPFAMPF02482Ribosomal_S30AEcoord: 100..196
e-value: 1.1E-22
score: 80.5
IPR003489Ribosome hibernation promoting factor/RaiACDDcd00552RaiAcoord: 98..196
e-value: 1.51836E-24
score: 93.3485
IPR032528Sigma 54 modulation/S30EA ribosomal protein, C-terminalPFAMPF16321Ribosom_S30AE_Ccoord: 237..290
e-value: 4.0E-26
score: 90.5
IPR038416Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamilyGENE3D3.30.505.50coord: 249..301
e-value: 1.9E-21
score: 77.4
IPR036567Ribosome hibernation promotion factor-likeGENE3D3.30.160.100coord: 98..201
e-value: 2.8E-22
score: 80.8
IPR036567Ribosome hibernation promotion factor-likeSUPERFAMILY69754Ribosome binding protein Y (YfiA homologue)coord: 98..203
NoneNo IPR availablePANTHERPTHR33231:SF130S RIBOSOMAL PROTEINcoord: 83..297
NoneNo IPR availablePANTHERPTHR3323130S RIBOSOMAL PROTEINcoord: 83..297
IPR034694Ribosome hibernation promoting factor, long/plastidHAMAPMF_00839HPFcoord: 97..294
score: 20.434986

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G000200.1CmoCh16G000200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045900 negative regulation of translational elongation
biological_process GO:0044238 primary metabolic process
cellular_component GO:0022627 cytosolic small ribosomal subunit
molecular_function GO:0043024 ribosomal small subunit binding
molecular_function GO:0043022 ribosome binding