CmoCh15G014930 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh15G014930
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Cmo_Chr15: 10117701 .. 10119068 (-)
RNA-Seq Expression	CmoCh15G014930
Synteny	CmoCh15G014930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCTCCGTTTCCCCTTTCTTCCATCTCATTCTTCTCCTCTTCTCTCTCGCCGATCTCCTCAACGCCGCCGCCGCCGCCGACTACCTGAAGCTTCCATTGCTCCACAAGAACCCCTTCTCCTCCCCTTCCCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTGCCCTCCGCCGCCGCCCTAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAGTACTTCGTCGACCTCCGCATTGGCACTCCTCCCCAGAGCCTCCTCCTCGTCGCCGATACTGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCTTTCCTCCCCCGTCACTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTCCCCCACGCTCCTCCCCACCTCTGTAACCACACGCGCCTCCACTCCCCCTGCCGCTTCCTCTACACTTACGCCGACGGCTCCACCTCCTCCGGCTTCTTCTCCAAGGAAACCACGACGTTGAAGACCCTCACTGGCTCCGAGACCCGTCTTAAAGATCTGTCGTTCGGGTGCGGCTTCCGGATCTCGGGTCCGAGCGTTTCGGGCGCCCAATTTAATGGAGCACGTGGTGTCATGGGATTGGGCAGAGGTCCGATCTCCTTCTCCACTCAACTCGGCCGCCGATTTGGCAATAAATTTTCCTATTGTCTTATGGATTACACACTCTCTCCGCCGCCCACCAGCTACCTAATGATCGGCGGCGGCCTCCGTAGACTCCCCGTGACCAATGCCACCAAAATCAGCTACACCCCATTGCTAATTAACCCTCTGTCACCAACATTCTACTACATTGCCGTCAAGAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCGACCTTGTGGGCCATCGACGAGCAAGGAAACGGCGGGACGGTGGTGGATTCGGGAACGACGCTGACGTATCTGGCAGAGGAGGCGTACAAGGAGGTGCTGAAGGCGATGAGACAGCGAGTGAAGCTACCGGCTGCAGCCGAGTTGACTCCGGGATTCGATCTATGCGTGAACGTATCAAACGAATCGCAGCGGCCGAGTCTACCACGAGTGAGATTCCAGCTGGGGAATGGTGCAGTGTTTCCGCCGCCGGCGAGGAACTACTTTCTGGAGACGGAGGAGGGGGTGATGTGCTTGGCGATCCGAGCAGTGGAGGGCGGAAATGGGTTCTCGGTGATTGGGAATCTGATGCAACAAGGATTCCTGCTGGAGTTCGACAAGGAGGCGTCGAGGCTGGGCTTCTCAAGGCGGGGCTGCGGGCTTCCTTGA

mRNA sequence

ATGCTCTCCGTTTCCCCTTTCTTCCATCTCATTCTTCTCCTCTTCTCTCTCGCCGATCTCCTCAACGCCGCCGCCGCCGCCGACTACCTGAAGCTTCCATTGCTCCACAAGAACCCCTTCTCCTCCCCTTCCCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTGCCCTCCGCCGCCGCCCTAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAGTACTTCGTCGACCTCCGCATTGGCACTCCTCCCCAGAGCCTCCTCCTCGTCGCCGATACTGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCTTTCCTCCCCCGTCACTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTCCCCCACGCTCCTCCCCACCTCTGTAACCACACGCGCCTCCACTCCCCCTGCCGCTTCCTCTACACTTACGCCGACGGCTCCACCTCCTCCGGCTTCTTCTCCAAGGAAACCACGACGTTGAAGACCCTCACTGGCTCCGAGACCCGTCTTAAAGATCTGTCGTTCGGGTGCGGCTTCCGGATCTCGGGTCCGAGCGTTTCGGGCGCCCAATTTAATGGAGCACGTGGTGTCATGGGATTGGGCAGAGGTCCGATCTCCTTCTCCACTCAACTCGGCCGCCGATTTGGCAATAAATTTTCCTATTGTCTTATGGATTACACACTCTCTCCGCCGCCCACCAGCTACCTAATGATCGGCGGCGGCCTCCGTAGACTCCCCGTGACCAATGCCACCAAAATCAGCTACACCCCATTGCTAATTAACCCTCTGTCACCAACATTCTACTACATTGCCGTCAAGAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCGACCTTGTGGGCCATCGACGAGCAAGGAAACGGCGGGACGGTGGTGGATTCGGGAACGACGCTGACGTATCTGGCAGAGGAGGCGTACAAGGAGGTGCTGAAGGCGATGAGACAGCGAGTGAAGCTACCGGCTGCAGCCGAGTTGACTCCGGGATTCGATCTATGCGTGAACGTATCAAACGAATCGCAGCGGCCGAGTCTACCACGAGTGAGATTCCAGCTGGGGAATGGTGCAGTGTTTCCGCCGCCGGCGAGGAACTACTTTCTGGAGACGGAGGAGGGGGTGATGTGCTTGGCGATCCGAGCAGTGGAGGGCGGAAATGGGTTCTCGGTGATTGGGAATCTGATGCAACAAGGATTCCTGCTGGAGTTCGACAAGGAGGCGTCGAGGCTGGGCTTCTCAAGGCGGGGCTGCGGGCTTCCTTGA

Coding sequence (CDS)

ATGCTCTCCGTTTCCCCTTTCTTCCATCTCATTCTTCTCCTCTTCTCTCTCGCCGATCTCCTCAACGCCGCCGCCGCCGCCGACTACCTGAAGCTTCCATTGCTCCACAAGAACCCCTTCTCCTCCCCTTCCCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTGCCCTCCGCCGCCGCCCTAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAGTACTTCGTCGACCTCCGCATTGGCACTCCTCCCCAGAGCCTCCTCCTCGTCGCCGATACTGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCTTTCCTCCCCCGTCACTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTCCCCCACGCTCCTCCCCACCTCTGTAACCACACGCGCCTCCACTCCCCCTGCCGCTTCCTCTACACTTACGCCGACGGCTCCACCTCCTCCGGCTTCTTCTCCAAGGAAACCACGACGTTGAAGACCCTCACTGGCTCCGAGACCCGTCTTAAAGATCTGTCGTTCGGGTGCGGCTTCCGGATCTCGGGTCCGAGCGTTTCGGGCGCCCAATTTAATGGAGCACGTGGTGTCATGGGATTGGGCAGAGGTCCGATCTCCTTCTCCACTCAACTCGGCCGCCGATTTGGCAATAAATTTTCCTATTGTCTTATGGATTACACACTCTCTCCGCCGCCCACCAGCTACCTAATGATCGGCGGCGGCCTCCGTAGACTCCCCGTGACCAATGCCACCAAAATCAGCTACACCCCATTGCTAATTAACCCTCTGTCACCAACATTCTACTACATTGCCGTCAAGAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCGACCTTGTGGGCCATCGACGAGCAAGGAAACGGCGGGACGGTGGTGGATTCGGGAACGACGCTGACGTATCTGGCAGAGGAGGCGTACAAGGAGGTGCTGAAGGCGATGAGACAGCGAGTGAAGCTACCGGCTGCAGCCGAGTTGACTCCGGGATTCGATCTATGCGTGAACGTATCAAACGAATCGCAGCGGCCGAGTCTACCACGAGTGAGATTCCAGCTGGGGAATGGTGCAGTGTTTCCGCCGCCGGCGAGGAACTACTTTCTGGAGACGGAGGAGGGGGTGATGTGCTTGGCGATCCGAGCAGTGGAGGGCGGAAATGGGTTCTCGGTGATTGGGAATCTGATGCAACAAGGATTCCTGCTGGAGTTCGACAAGGAGGCGTCGAGGCTGGGCTTCTCAAGGCGGGGCTGCGGGCTTCCTTGA

Protein sequence

MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP

Homology

[Close]

BLAST of CmoCh15G014930 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 4.9e-63
Identity = 154/436 (35.32%), Postives = 225/436 (51.61%), Query Frame = 0


Query: 28  DYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTL---------KSPLISGAS 87
           D++     +K P    S  L  D+ R+  + +   + P   +          S ++SG S
Sbjct: 77  DHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLS 136

Query: 88  TGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPF 147
            GSG+YF  L +GTP + + +V DTGSD+VW++C+ CR C +      F PR S +++  
Sbjct: 137 QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPRKSKTYATI 196

Query: 148 HCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLTGSETRL 207
            C  PHCR L  A    CN  R    C +  +Y DGS + G FS ET T +       R+
Sbjct: 197 PCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRV 256

Query: 208 KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSP 267
           K ++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S 
Sbjct: 257 KGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 316

Query: 268 PPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLP-INPTLW 327
            P+S +     + R+         +TPLL NP   TFYY+ +  I+V G ++P +  +L+
Sbjct: 317 KPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLF 376

Query: 328 AIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQ 387
            +D+ GNGG ++DSGT++T L   AY  +  A R   K    A     FD C ++SN ++
Sbjct: 377 KLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNE 436

Query: 388 RPSLPRVRFQLGNGAVFPPPARNYFLETE-EGVMCLAIRAVEGGNGFSVIGNLMQQGFLL 447
              +P V      GA    PA NY +  +  G  C A     G  G S+IGN+ QQGF +
Sbjct: 437 -VKVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRV 484

Query: 448 EFDKEASRLGFSRRGC 453
            +D  +SR+GF+  GC
Sbjct: 497 VYDLASSRVGFAPGGC 484

[Close]

BLAST of CmoCh15G014930 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.9e-54
Identity = 129/374 (34.49%), Postives = 198/374 (52.94%), Query Frame = 0


Query: 80  GSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 139
           G G+Y +++ IGTP  S   + DTGSDL+W +C  C  C    P+  F P+ SSSFS   
Sbjct: 92  GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQC-FSQPTPIFNPQDSSSFSTLP 151

Query: 140 CFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLTGSETRLK 199
           C   +C+ L   P   CN    ++ C++ Y Y DGST+ G+ + ET T +T     + + 
Sbjct: 152 CESQYCQDL---PSETCN----NNECQYTYGYGDGSTTQGYMATETFTFET-----SSVP 211

Query: 200 DLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPP 259
           +++FGCG    G      Q NGA G++G+G GP+S  +QLG     +FSYC+  Y  S P
Sbjct: 212 NIAFGCGEDNQG----FGQGNGA-GLIGMGWGPLSLPSQLG---VGQFSYCMTSYGSSSP 271

Query: 260 PTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPTLWAI 319
            T  L +G     +P  + +    T L+ + L+PT+YYI ++ ITV G  L I  + + +
Sbjct: 272 ST--LALGSAASGVPEGSPS----TTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQL 331

Query: 320 DEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQRP 379
            + G GG ++DSGTTLTYL ++AY  V +A   ++ LP   E + G   C    ++    
Sbjct: 332 QDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTV 391

Query: 380 SLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEFD 439
            +P +  Q  +G V     +N  +   EGV+CLA+ +     G S+ GN+ QQ   + +D
Sbjct: 392 QVPEISMQF-DGGVLNLGEQNILISPAEGVICLAMGS-SSQLGISIFGNIQQQETQVLYD 436

Query: 440 KEASRLGFSRRGCG 454
            +   + F    CG
Sbjct: 452 LQNLAVSFVPTQCG 436

[Close]

BLAST of CmoCh15G014930 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.9e-51
Identity = 148/450 (32.89%), Postives = 219/450 (48.67%), Query Frame = 0


Query: 12  LLLFSLADLLNAAAAADYLKLPLLHKN-----PFSSPSQALSSDTHRLSLLFSALRRRPN 71
           LLL ++AD +   +    ++L L H++     P S     + +D  R SL+  + +R   
Sbjct: 35  LLLITVADSMKDTS----VRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLI--SRKRNST 94

Query: 72  PTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSA 131
             +K  L SG   G+ QYF ++R+GTP +   +V DTGS+L WV C              
Sbjct: 95  VGVKMDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCR--YRARGKDNRRV 154

Query: 132 FLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETT 191
           F    S SF    C    C++       L       +PC + Y YADGS + G F+KET 
Sbjct: 155 FRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETI 214

Query: 192 TLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNK 251
           T+    G   RL     GC       S +G  F GA GV+GL     SF++     +G K
Sbjct: 215 TVGLTNGRMARLPGHLIGC-----SSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAK 274

Query: 252 FSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISY---TPLLINPLSPTFYYIAVKSI 311
           FSYCL+D+  +   ++YL+ G        + +TK ++   TPL +  + P FY I V  I
Sbjct: 275 FSYCLVDHLSNKNVSNYLIFGS-------SRSTKTAFRRTTPLDLTRI-PPFYAINVIGI 334

Query: 312 TVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAM-RQRVKLPAAAEL 371
           ++    L I   +W  D    GGT++DSGT+LT LA+ AYK+V+  + R  V+L      
Sbjct: 335 SLGYDMLDIPSQVW--DATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPE 394

Query: 372 TPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGGNG 431
               + C + ++      LP++ F L  GA F P  ++Y ++   GV CL   +  G   
Sbjct: 395 GVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSA-GTPA 454

Query: 432 FSVIGNLMQQGFLLEFDKEASRLGFSRRGC 453
            +VIGN+MQQ +L EFD  AS L F+   C
Sbjct: 455 TNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of CmoCh15G014930 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.9e-51
Identity = 127/375 (33.87%), Postives = 190/375 (50.67%), Query Frame = 0


Query: 80  GSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 139
           G G+Y ++L IGTP Q    + DTGSDL+W +C  C  C  +  +  F P+ SSSFS   
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQC-FNQSTPIFNPQGSSSFSTLP 150

Query: 140 CFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLTGSETRLK 199
           C    C+ L  + P   N+      C++ Y Y DGS + G    E     TLT     + 
Sbjct: 151 CSSQLCQAL--SSPTCSNNF-----CQYTYGYGDGSETQGSMGTE-----TLTFGSVSIP 210

Query: 200 DLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPP 259
           +++FGCG    G      Q NGA G++G+GRGP+S  +QL      KFSYC+     S P
Sbjct: 211 NITFGCGENNQG----FGQGNGA-GLVGMGRGPLSLPSQLD---VTKFSYCMTPIGSSTP 270

Query: 260 PTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPTLWAI 319
             S L++G     +   +      T L+ +   PTFYYI +  ++V   +LPI+P+ +A+
Sbjct: 271 --SNLLLGSLANSVTAGSPN----TTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFAL 330

Query: 320 D-EQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQR 379
           +   G GG ++DSGTTLTY    AY+ V +    ++ LP     + GFDLC    ++   
Sbjct: 331 NSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSN 390

Query: 380 PSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEF 439
             +P       +G     P+ NYF+    G++CLA+ +     G S+ GN+ QQ  L+ +
Sbjct: 391 LQIPTFVMHF-DGGDLELPSENYFISPSNGLICLAMGS--SSQGMSIFGNIQQQNMLVVY 435

Query: 440 DKEASRLGFSRRGCG 454
           D   S + F+   CG
Sbjct: 451 DTGNSVVSFASAQCG 435

[Close]

BLAST of CmoCh15G014930 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.6e-50
Identity = 140/441 (31.75%), Postives = 219/441 (49.66%), Query Frame = 0


Query: 32  LPLLHKNPFSSPS---------QALSSDTHRLSLLFSALRRRPNPT---------LKSPL 91
           L LLH++ F S +           +  DT R+S +   +  +  P+           S +
Sbjct: 61  LRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDI 120

Query: 92  ISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSS 151
           +SG   GSG+YFV + +G+PP+   +V D+GSD+VWV+C  C+ C +      F P  S 
Sbjct: 121 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC-YKQSDPVFDPAKSG 180

Query: 152 SFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLTG 211
           S++   C    C  + ++  H          CR+   Y DGS     ++K T  L+TLT 
Sbjct: 181 SYTGVSCGSSVCDRIENSGCH-------SGGCRYEVMYGDGS-----YTKGTLALETLTF 240

Query: 212 SETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMD 271
           ++T +++++ GCG R  G       F GA G++G+G G +SF  QL  + G  F YCL+ 
Sbjct: 241 AKTVVRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLV- 300

Query: 272 YTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPIN 331
            +     T  L+   G   LPV      S+ PL+ NP +P+FYY+ +K + V GV++P+ 
Sbjct: 301 -SRGTDSTGSLVF--GREALPV----GASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLP 360

Query: 332 PTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMR-QRVKLPAAAELTPGFDLCVNV 391
             ++ + E G+GG V+D+GT +T L   AY       + Q   LP A+ ++  FD C ++
Sbjct: 361 DGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FDTCYDL 420

Query: 392 SNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEE-GVMCLAIRAVEGGNGFSVIGNLMQ 451
           S       +P V F    G V   PARN+ +  ++ G  C A  A     G S+IGN+ Q
Sbjct: 421 SG-FVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAA--SPTGLSIIGNIQQ 470

Query: 452 QGFLLEFDKEASRLGFSRRGC 453
           +G  + FD     +GF    C
Sbjct: 481 EGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of CmoCh15G014930 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 1.9e-264
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0


Query: 1   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60
           MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 5   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 64

Query: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 65  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 124

Query: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 125 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 184

Query: 181 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240
           FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 185 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 244

Query: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 300
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 245 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 304

Query: 301 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360
           KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA
Sbjct: 305 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 364

Query: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 420
           ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG
Sbjct: 365 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 424

Query: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 425 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 2.7e-258
Identity = 445/455 (97.80%), Postives = 450/455 (98.90%), Query Frame = 0


Query: 1   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60
           MLSVS FFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 5   MLSVSTFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 64

Query: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 65  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 124

Query: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 125 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 184

Query: 181 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240
           FSKETTTLKTL+GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 185 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 244

Query: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 300
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLR LPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 245 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 304

Query: 301 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360
           KSITVDGVKLPINPT+WAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKA+RQRVKLPAAA
Sbjct: 305 KSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAA 364

Query: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 420
           ELTPGFDLCVNVS ESQRPSLPRVRF++GNGAVF PPARNYFLET EGVMCLAIRAVEGG
Sbjct: 365 ELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVEGG 424

Query: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 425 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.1e-226
Identity = 395/461 (85.68%), Postives = 419/461 (90.89%), Query Frame = 0


Query: 1   MLSVSPFFHLILLL-FSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 60
           +LS+SPFF LILL  F L  L N      AA AD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 3   VLSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRL 62

Query: 61  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 120
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122

Query: 121 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 180
           CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADG
Sbjct: 123 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADG 182

Query: 181 STSSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 240
           S SSGFFSKETTTLK+L+GSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 183 SLSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242

Query: 241 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPT 300
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL  LP+TNATKISYTPL INPLSPT
Sbjct: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPT 302

Query: 301 FYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV 360
           FYYI + SIT+DGVKLPINP +W IDEQGNGGTVVDSGTTLTYL + AY+EVLK++R+RV
Sbjct: 303 FYYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362

Query: 361 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAI 420
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVF PP RNYFLETEEGVMCLAI
Sbjct: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422

Query: 421 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.1e-226
Identity = 395/461 (85.68%), Postives = 416/461 (90.24%), Query Frame = 0


Query: 1   MLSVSPFFHLI-LLLFSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 60
           MLS+SPFF LI L  F L  L N      AAAAD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62

Query: 61  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 120
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122

Query: 121 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 180
           CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAPPH CNHT LHSPCRFLY+YADG
Sbjct: 123 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADG 182

Query: 181 STSSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 240
           S SSGFFSKETTTLKTL+GSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 183 SLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242

Query: 241 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPT 300
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL  LPV NATKISYTPL INPLSPT
Sbjct: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPT 302

Query: 301 FYYIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV 360
           FYYI + SIT+DGVKLPINP +W IDEQGNGGTVVDSGTTLTYL + AY+EVLK++R+RV
Sbjct: 303 FYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362

Query: 361 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAI 420
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVF PP RNYFLETEEGVMCLAI
Sbjct: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422

Query: 421 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.2e-213
Identity = 371/448 (82.81%), Postives = 399/448 (89.06%), Query Frame = 0


Query: 10  LILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTL 69
           L+LLL SLADL N A  + YLK PLLH NPFSSPSQALSSDTHRLSLLFSA   R +PTL
Sbjct: 11  LLLLLSSLADLSN-AIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSA--HRHSPTL 70

Query: 70  KSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLP 129
           KSPLISGASTGSGQYFV+L +GTPPQSLLLV DTGSDLVWVKCS CRNCSHHPPSSAF P
Sbjct: 71  KSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCSHHPPSSAFFP 130

Query: 130 RHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLK 189
           RHSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLY+YAD S SSGFFSK+ TT  
Sbjct: 131 RHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSGFFSKDVTTFN 190

Query: 190 TLTG--SETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKF 249
           T +G  ++TRL DLSFGCGFRISGPSVSGA+F GARGVMGLGRGPISFS+QLG RFGN F
Sbjct: 191 TFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSSQLGHRFGNTF 250

Query: 250 SYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDG 309
           SYCLMDYTLSPPPTSYLMIGGGLR LPVTNA+KISYTPL INPLSPTFYYI VKSITVDG
Sbjct: 251 SYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDG 310

Query: 310 VKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFD 369
           VKLPINP +WAIDEQGNGGTVVDSGTTLTYLAE AY+EVLKAMR+RVKLP A +L+PGFD
Sbjct: 311 VKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFD 370

Query: 370 LCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGGNGFSVIG 429
           LCVN S+ES+  SLP++RF++G G VF PPARNYF+ETEEGVMCLAIR V+ GNGFSVIG
Sbjct: 371 LCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIG 430

Query: 430 NLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NLMQQGFLLEFD+E SR+GFSRRGCGLP
Sbjct: 431 NLMQQGFLLEFDREKSRMGFSRRGCGLP 455

[Close]

BLAST of CmoCh15G014930 vs. NCBI nr
Match: XP_022928946.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 921.0 bits (2379), Expect = 4.0e-264
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0


Query: 1   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60
           MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 5   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 64

Query: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 65  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 124

Query: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 125 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 184

Query: 181 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240
           FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 185 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 244

Query: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 300
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 245 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 304

Query: 301 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360
           KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA
Sbjct: 305 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 364

Query: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 420
           ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG
Sbjct: 365 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 424

Query: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 425 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 908.7 bits (2347), Expect = 2.1e-260
Identity = 448/455 (98.46%), Postives = 452/455 (99.34%), Query Frame = 0


Query: 1   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60
           MLSVSPFFH ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 5   MLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 64

Query: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 65  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 124

Query: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 125 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 184

Query: 181 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240
           FSKETTTLKTL+GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 185 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 244

Query: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 300
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLR LPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 245 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 304

Query: 301 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360
           KSITVDGVKLPINPT+WAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKA+RQRVKLPAAA
Sbjct: 305 KSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAA 364

Query: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 420
           ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVF PPARNYFLETEEGVMCL+IRAVEGG
Sbjct: 365 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLETEEGVMCLSIRAVEGG 424

Query: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 425 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. NCBI nr
Match: KAG7017139.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 906.0 bits (2340), Expect = 1.3e-259
Identity = 447/455 (98.24%), Postives = 451/455 (99.12%), Query Frame = 0


Query: 1   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60
           MLSVSPFFH ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 1   MLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60

Query: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120

Query: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180

Query: 181 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240
           FSKETTTLKTL+GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 181 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240

Query: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 300
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLR LPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 300

Query: 301 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360
           KSITVDGVKLPINP +WAI+EQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA
Sbjct: 301 KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360

Query: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 420
           ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVF PPARNYFL+TEEGVMCLAIRAVEGG
Sbjct: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG 420

Query: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 455

[Close]

BLAST of CmoCh15G014930 vs. NCBI nr
Match: KAG6579697.1 (Aspartic proteinase NANA, chloroplast, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 906.0 bits (2340), Expect = 1.3e-259
Identity = 447/455 (98.24%), Postives = 451/455 (99.12%), Query Frame = 0


Query: 1   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60
           MLSVSPFFH ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 5   MLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 64

Query: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 65  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 124

Query: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 125 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 184

Query: 181 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240
           FSKETTTLKTL+GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 185 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 244

Query: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 300
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLR LPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 245 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 304

Query: 301 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360
           KSITVDGVKLPINP +WAI+EQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA
Sbjct: 305 KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 364

Query: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 420
           ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVF PPARNYFL+TEEGVMCLAIRAVEGG
Sbjct: 365 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG 424

Query: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 425 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. NCBI nr
Match: XP_022969957.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 900.6 bits (2326), Expect = 5.6e-258
Identity = 445/455 (97.80%), Postives = 450/455 (98.90%), Query Frame = 0


Query: 1   MLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60
           MLSVS FFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 5   MLSVSTFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 64

Query: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 65  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 124

Query: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 125 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 184

Query: 181 FSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240
           FSKETTTLKTL+GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 185 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 244

Query: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAV 300
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLR LPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 245 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 304

Query: 301 KSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360
           KSITVDGVKLPINPT+WAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKA+RQRVKLPAAA
Sbjct: 305 KSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAA 364

Query: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 420
           ELTPGFDLCVNVS ESQRPSLPRVRF++GNGAVF PPARNYFLET EGVMCLAIRAVEGG
Sbjct: 365 ELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVEGG 424

Query: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 425 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]

BLAST of CmoCh15G014930 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 602.4 bits (1552), Expect = 2.9e-172
Identity = 303/460 (65.87%), Postives = 362/460 (78.70%), Query Frame = 0


Query: 8   FHLILLLFSLADLL-----NAAAAAD---YLKLPLLHKNPFSSPSQALSSDTHRLSLLFS 67
           F LI  L S   L      N AA ++   YLKLPLL K+PF SP+QAL+ DT RL   F 
Sbjct: 2   FPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLH--FL 61

Query: 68  ALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCS 127
           +LRR+P P +KSP++SGA++GSGQYFVDLRIG PPQSLLL+ADTGSDLVWVKCSACRNCS
Sbjct: 62  SLRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCS 121

Query: 128 HHPPSSAFLPRHSSSFSPFHCFDPHCRLLP---HAPPHLCNHTRLHSPCRFLYTYADGST 187
           HH P++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGS 
Sbjct: 122 HHSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSL 181

Query: 188 SSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 247
           +SG F++ETT+LKT +G E RLK ++FGCGFRISG SVSG  FNGA GVMGLGRGPISF+
Sbjct: 182 TSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFA 241

Query: 248 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFY 307
           +QLGRRFGNKFSYCLMDYTLSPPPTSYL+IG G         +K+ +TPLL NPLSPTFY
Sbjct: 242 SQLGRRFGNKFSYCLMDYTLSPPPTSYLIIGNG-----GDGISKLFFTPLLTNPLSPTFY 301

Query: 308 YIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 367
           Y+ +KS+ V+G KL I+P++W ID+ GNGGTVVDSGTTL +LAE AY+ V+ A+R+RVKL
Sbjct: 302 YVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKL 361

Query: 368 PAAAELTPGFDLCVNVSNESQ-RPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIR 427
           P A  LTPGFDLCVNVS  ++    LPR++F+   GAVF PP RNYF+ETEE + CLAI+
Sbjct: 362 PIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQ 421

Query: 428 AVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           +V+   GFSVIGNLMQQGFL EFD++ SRLGFSRRGC LP
Sbjct: 422 SVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

[Close]

BLAST of CmoCh15G014930 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 407.5 bits (1046), Expect = 1.4e-113
Identity = 231/460 (50.22%), Postives = 276/460 (60.00%), Query Frame = 0


Query: 8   FHLILLLFSLADLL-----NAAAAAD---YLKLPLLHKNPFSSPSQALSSDTHRLSLLFS 67
           F LI  L S   L      N AA ++   YLKLPLL K+PF SP+QAL+ DT RL   F 
Sbjct: 2   FPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLH--FL 61

Query: 68  ALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCS 127
           +LRR+P P +KSP++SGA++GSGQYFVDLRIG PPQSLLL+ADTGSDLVWVKCSACRNCS
Sbjct: 62  SLRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCS 121

Query: 128 HHPPSSAFLPRHSSSFSPFHCFDPHCRLLP---HAPPHLCNHTRLHSPCRFLYTYADGST 187
           HH P++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGS 
Sbjct: 122 HHSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSL 181

Query: 188 SSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 247
           +SG F++ETT+LKT +G E RLK ++FGCGFRISG SVS                     
Sbjct: 182 TSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVS--------------------- 241

Query: 248 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFY 307
                                                                       
Sbjct: 242 ------------------------------------------------------------ 301

Query: 308 YIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 367
                                     GNGGTVVDSGTTL +LAE AY+ V+ A+R+RVKL
Sbjct: 302 --------------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKL 350

Query: 368 PAAAELTPGFDLCVNVSNESQ-RPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIR 427
           P A  LTPGFDLCVNVS  ++    LPR++F+   GAVF PP RNYF+ETEE + CLAI+
Sbjct: 362 PIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQ 350

Query: 428 AVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 456
           +V+   GFSVIGNLMQQGFL EFD++ SRLGFSRRGC LP
Sbjct: 422 SVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

[Close]

BLAST of CmoCh15G014930 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-64
Identity = 152/392 (38.78%), Postives = 212/392 (54.08%), Query Frame = 0


Query: 69  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 128
           L + L SG + GSG+YF+D+ +GTPP+   L+ DTGSDL W++C  C +C  H     + 
Sbjct: 145 LIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDC-FHQNGMFYD 204

Query: 129 PRHSSSFSPFHCFDPHCRLLPHA-PPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTT 188
           P+ S+SF    C DP C L+    PP  C     +  C + Y Y D S ++G F+ ET T
Sbjct: 205 PKTSASFKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYWYGDRSNTTGDFAVETFT 264

Query: 189 LKTLT----GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRF 248
           +   T     SE ++ ++ FGCG    G       F+GA G++GLGRGP+SFS+QL   +
Sbjct: 265 VNLTTTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSLY 324

Query: 249 GNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSI 308
           G+ FSYCL+D   +   +S L+ G     L  TN    S+     N +  TFYYI +KSI
Sbjct: 325 GHSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVE-TFYYIQIKSI 384

Query: 309 TVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVK--LPAAAE 368
            V G  L I    W I   G+GGT++DSGTTL+Y AE AY+ +     +++K   P   +
Sbjct: 385 LVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRD 444

Query: 369 LTPGFDLCVNVSN-ESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 428
             P  D C NVS  E     LP +     +G V+  PA N F+   E ++CLAI      
Sbjct: 445 F-PVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKS 504

Query: 429 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGC 453
             FS+IGN  QQ F + +D + SRLGF+   C
Sbjct: 505 T-FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

[Close]

BLAST of CmoCh15G014930 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 243.4 bits (620), Expect = 3.5e-64
Identity = 154/436 (35.32%), Postives = 225/436 (51.61%), Query Frame = 0


Query: 28  DYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTL---------KSPLISGAS 87
           D++     +K P    S  L  D+ R+  + +   + P   +          S ++SG S
Sbjct: 77  DHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLS 136

Query: 88  TGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPF 147
            GSG+YF  L +GTP + + +V DTGSD+VW++C+ CR C +      F PR S +++  
Sbjct: 137 QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPRKSKTYATI 196

Query: 148 HCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLTGSETRL 207
            C  PHCR L  A    CN  R    C +  +Y DGS + G FS ET T +       R+
Sbjct: 197 PCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRV 256

Query: 208 KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSP 267
           K ++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S 
Sbjct: 257 KGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 316

Query: 268 PPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLP-INPTLW 327
            P+S +     + R+         +TPLL NP   TFYY+ +  I+V G ++P +  +L+
Sbjct: 317 KPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLF 376

Query: 328 AIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQ 387
            +D+ GNGG ++DSGT++T L   AY  +  A R   K    A     FD C ++SN ++
Sbjct: 377 KLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNE 436

Query: 388 RPSLPRVRFQLGNGAVFPPPARNYFLETE-EGVMCLAIRAVEGGNGFSVIGNLMQQGFLL 447
              +P V      GA    PA NY +  +  G  C A     G  G S+IGN+ QQGF +
Sbjct: 437 -VKVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRV 484

Query: 448 EFDKEASRLGFSRRGC 453
            +D  +SR+GF+  GC
Sbjct: 497 VYDLASSRVGFAPGGC 484

[Close]

BLAST of CmoCh15G014930 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 228.0 bits (580), Expect = 1.5e-59
Identity = 146/392 (37.24%), Postives = 209/392 (53.32%), Query Frame = 0


Query: 69  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 128
           L + L SG + GSG+YF+D+ +G+PP+   L+ DTGSDL W++C  C +C     + AF 
Sbjct: 155 LVATLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQ--NGAFY 214

Query: 129 -PRHSSSFSPFHCFDPHCRLLPHA-PPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETT 188
            P+ S+S+    C D  C L+    PP  C     +  C + Y Y D S ++G F+ ET 
Sbjct: 215 DPKASASYKNITCNDQRCNLVSSPDPPMPCKSD--NQSCPYYYWYGDSSNTTGDFAVETF 274

Query: 189 TLK-TLTGSETRL---KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRR 248
           T+  T  G  + L   +++ FGCG    G       F+GA G++GLGRGP+SFS+QL   
Sbjct: 275 TVNLTTNGGSSELYNVENMMFGCGHWNRG------LFHGAAGLLGLGRGPLSFSSQLQSL 334

Query: 249 FGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFYYIAVKS 308
           +G+ FSYCL+D       +S L+ G     L   N    S+     N L  TFYY+ +KS
Sbjct: 335 YGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKEN-LVDTFYYVQIKS 394

Query: 309 ITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV--KLPAAA 368
           I V G  L I    W I   G GGT++DSGTTL+Y AE AY+ +   + ++   K P   
Sbjct: 395 ILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYR 454

Query: 369 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGG 428
           +  P  D C NVS       LP +     +GAV+  P  N F+   E ++CLA+      
Sbjct: 455 DF-PILDPCFNVSG-IHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTP-K 514

Query: 429 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGC 453
           + FS+IGN  QQ F + +D + SRLG++   C
Sbjct: 515 SAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

The following BLAST results are available for this feature:

BLAST of CmoCh15G014930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNJ3	4.9e-63	35.32	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	1.9e-54	34.49	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LTW4	1.9e-51	32.89	Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE...	[more]
Q766C3	1.9e-51	33.87	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LHE3	1.6e-50	31.75	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of CmoCh15G014930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ELQ4	1.9e-264	100.00	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HXS2	2.7e-258	97.80	aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 P...	[more]
A0A0A0KNH6	2.1e-226	85.68	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G17465...	[more]
A0A1S3CSZ8	2.1e-226	85.68	aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 ...	[more]
A0A6J1EPZ3	1.2e-213	82.81	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of CmoCh15G014930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022928946.1	4.0e-264	100.00	aspartyl protease family protein 2-like [Cucurbita moschata]	[more]
XP_023549997.1	2.1e-260	98.46	aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo]	[more]
KAG7017139.1	1.3e-259	98.24	Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6579697.1	1.3e-259	98.24	Aspartic proteinase NANA, chloroplast, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022969957.1	5.6e-258	97.80	aspartyl protease family protein 2 [Cucurbita maxima]	[more]

BLAST of CmoCh15G014930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25700.1	2.9e-172	65.87	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.2	1.4e-113	50.22	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	2.6e-64	38.78	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	3.5e-64	35.32	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	1.5e-59	37.24	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 327..338 score: 41.24 coord: 90..110 score: 51.9 coord: 424..439 score: 18.99
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 84..268 e-value: 6.8E-49 score: 166.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 62..267 e-value: 1.2E-46 score: 161.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 273..453 e-value: 8.6E-53 score: 180.8
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 78..452
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 295..448 e-value: 1.8E-37 score: 128.6
None	No IPR available	PANTHER	PTHR47967:SF72	BNAA02G27600D PROTEIN	coord: 12..453
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 12..453
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 84..448 score: 41.398293
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 83..452 e-value: 1.55584E-83 score: 256.036

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh15G014930.1	CmoCh15G014930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity