CmoCh15G013760 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G013760
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationCmo_Chr15: 9374276 .. 9378361 (+)
RNA-Seq ExpressionCmoCh15G013760
SyntenyCmoCh15G013760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTGCGGTTCGGATTTGATGTCTGGGAACTCCGAGGCTGATGATCGTGAGATTGTAGAGAAGGATCCAACAGGACGATACATTCGGGTATTTCCTTTTAATTCTCATCTTCGATTTCGTTTGGATATTCTTATTGCCCTAGCGACTGGGATAGAACAGTTCTTTTTTAGAAAAACCCCATTCCTTATTTTCTATTTTTGACATTGTTTCCTTCACTGTTTTGGTTACTTAATTTTAATTTTAATATACGAATCATCTAATTTATCGATTGAATTGCTTTGAATTTGCGCATAATTACCAAATCGCCAATAGTTCATGATCGACTAATGTTTCAGATGTGTTCTTTACCTTTTGTAGTTCATATGTTTGATTCTATTTGACTTTATGATTCAATCCTCATTTAGACTTGTTTTATTTTTCAGTACGATGAGATTTTGGGTAAAGGGGCATTCAAGACAGTGTATGTTTCTGATGTCTATCAATCTCTGTTCTTTTATTACCTTTTCTTTTCTAGTTTCTTCATTATTGTGAGATGGTTTTTTTGGGTGTAATGAATGTTTGGGTGTGTTATTTGACTTGAGGGCTCTTTTTGTTGTCATCAGATACAGAGCATTCGACGAAGTCGGTGGAATCGAAGTTGCTTGGAGCCAAGTTGACATTGAAGATGTTTTGCAGTCACCAGAACAGCTTCAGAGATTATATTCTGAGGTTCATCTGCTGAAGTCTCTGAAACATGAAAATATAATCAAATTCTATTGCTATTGGGTTGATGATAAGAACAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCAGTAAGTTCCTAAGAGTAGTCTTCTTTTTGTTTTCGGAATCATTTTACTGCATTCAACGAGTTGTTTATTCGTTTATGGTATTGTTTGCAATTTAGATATCGAAGGAAGCATAGGAAAGTTGACTTGAAAGCCTTCAAGAATTGGGCGAGGCAGATACTGCGAGGCTTGACGTATTTACATGGTCACAACCCTCCAATTATTCATAGGGATTTGAAATGTGATAATATATTTGTCAATGGAAATACGGGAGATGTTAAAATTGGAGATCTCGGTTTGGCGATTGTCATGCAGCAGCCTACTGCTCGAAGTGTTATTGGTAATAATTTCGTTTTCTAGCTCGATATCTGTTTGTTGTGGGGATAGAACTCTAGGATTCATCTGATATTTGAGATGTGATTATTATTCAGGCACTCCAGAGTTCATGGCTCCAGAACTTTATGATGAGGAGTATAATGAGCTTGTTGATATCTATTCTTTTGGCATGTGCATGTTGGAAATTGTGACTTGTGAATACCCATATAGTGAGTGCAAAAATTCTGCCCAAATATTCAAGAAGGTTACTTCGGTAAGTAAATGATTTACTTCTACCAGATTATGTCCGTTAAATGTTTCTATTTGTGAGATCTTACATCGGTTGGAGAGGGAAACGAAGCATTCCTTATAAGGGTGTGAAACCTCTCCTTAGCAGACGCGTTTTAAAAACCTTGAGGGAAAGCCTAAAGAGAACAATATATGCTTTCGGTGGGCTTGTGCTGTTACAAATGGTATCAGAGCCAGACATCGAGCGGTGTGCCAACGAGGACATTGGGCCCTCAAGGGGGTGGATTGTGAGATCCACATCGGTTGGAAAGGGGACCAAAGCATTCCTTATAAGGGTATGGAAACCTCTCCCTAGTATATGCGTTTTAAAAACCTTGAGAGGAAGCTTGAAAAGGAAAGTTCAAAGAGGACAATATTTGCTTGCGGTGGGTTTGGGCTGTTACAAATGGTATCAGAGCCAAACATTGGACGGTGTGCCAACGAGGATGCTATGTCCTCAAGGGGGGTGGATTGTGAGATCTCATATCGGTTGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGAAAACTTCTCCCTAGCAGACCCGTTTTAAAAACTTTGAGGGGAAGCCCGAAAGGGAAATCCCGAAGAGAACAATATCAAATATCGGCTTGCGGTGGGCTGAAATCATTTCTTCTTTCTTGCAGGGTATAAAGCCTGCTTCCCTCGAAAAAGTGCTGGATCCCCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCCTTCCTTGCAACTGAAAGTCCCAAGGAGCATAGTTTTGAACCTTTAAGGTTATCGAATGAGCCGTTCAAATCTGAAAATCCTCCTGTGCTAGCTTCTCATTCAATGGACACAGACCATAACTGCAGCAAATTCTCAGGCTCAGTGGTCTCATCTGTCAAAAGCGATAACGACATTTCTCAGTTTTCAACTCTCGAACTTCAGAGGCTCACCGAGACTAATGAATTGACGCTAAAAGGGGACATGACTGATCATAATACAATTTCGTTCCATTTGCGCATTGCTGAACTAAGCGGTAACTTTCGGTCGATTCTTTCTTAATCTCCTTAAGTTGTTTATTCTATAGTGAATGTGTTTATATTCTTCATGATAACTCGAACATCTCTTTTCATATAGGTAAATCGAAGAATATCCATTTTGCCTTCTATCTCGACTCTGATACTTCATTGGCTATTGTTTTGGAGATGGTTGAACAACTCGAACTGTCAAATGAAGACGCGACTGTCATAGCGAAATTAATCGATGAATTAATTGCCAAGTTTGTTCCCGACTCGAAACCATGTCCAAACTATTATCACGGACAAAACACTACACACAGTTCTTCTGAAAGTGAAACTCAAGAGGACAAGACCTTCATTCCTCCTTTCTTCTCTGAGATGGTACTATCATCTCCCATGGTGGCTGGTGCAGGGAACAACCCGACGCATTTCACCAAGGTGGAAGATCAAGAAAACCTACAGTCGATCATTTCGTGTGCATCGCTCGACTTCAATCACTCAACTATCTCAGATTACAGCACAGGAAAAGGAACAATGGATTTCGATGCTGTTGGATCTATTTCGACGATGATCGATTTCGCAAACCCATCTCTAATCTCCTCCTGCTCAGGAATGTCGAAAGAAATGAGTTCGTCGAACTTTTCGAGCCTTTCTCTGGAAGAAAGAGATCACCAAGATGAACTGAAGATGGAGATTGATGCCATTGACTTACAGTACCATCAGTGCCTTTGTGAACTGTCAAGAATGAGAGAGGAAGCAATAGAAAGTGCGAAGAAGAGGTGGATGTCAAAGAAGAAAGCTCCTGCTTTTTGAAGCCTTCCTTTTTCCTTTGGATTTGCAAATTCTCTTTCTTTCTTGGCAAACTCTTGCTTGCAGAATGGTAACTTTTCTTTTCATTTTTTGTTTCTAAACTACTGACTGACTGCTATTCTTTGCATTCTATAACCAACTAAGTCCTTTTAAGTCCTAACGTCTGACTCTCCGATTATCGACATTCAATTCGAAAAGATCCTTATATAACGTTATTGAATCTCTAGCCAACAACACGCTTTGCATCCTGTTGTTGGGCTATGTATGTACCTCATGAATGTAGTAGAAATATCGACCGAATAAATGATCATGAATCGATGGAATTCGAGCCTTCAACAGGTAGAAGGAGAGACAGAGATGACGGTCTGCCGAAATTCGAATGAAAAAAAACAAAACATGAATCATATGATGAACCGTGTATCAGAATTTTAGAACATTAACTAAAGGTTTGAAAATGATTTGATCCACAGGAAGGTGTCTGGGAAACTGGTGGAGAGGTTATGATCTACACGAGAAAGGAGAATGAACCAATTTTGAACTTGATTCTGCTGCTCACGAGAGATATGAGGGAAGTTTTTAATACTACTTTTGTTCAACTATGGTGTTGCCTTTGCTGCTCTTTCTTTCTGCTATCGTCGTGTTATTGAGGGTTGGAACGATGATTTAGTCTCCGAACCATATTAGTATTTATTTCGTTTTATCTTTAAACTTTAAAGATTTTTATGTCGATAATACATAAATTAATAAATATTGGTTGATTTGATATGACGTGACAGATAGATGATATAACGAACAAATAAATTGGTTGATTTGATATGGGGC

mRNA sequence

ATGAGTTGCGGTTCGGATTTGATGTCTGGGAACTCCGAGGCTGATGATCGTGAGATTGTAGAGAAGGATCCAACAGGACGATACATTCGGTACGATGAGATTTTGGGTAAAGGGGCATTCAAGACAGTATACAGAGCATTCGACGAAGTCGGTGGAATCGAAGTTGCTTGGAGCCAAGTTGACATTGAAGATGTTTTGCAGTCACCAGAACAGCTTCAGAGATTATATTCTGAGGTTCATCTGCTGAAGTCTCTGAAACATGAAAATATAATCAAATTCTATTGCTATTGGGTTGATGATAAGAACAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAGGAAGCATAGGAAAGTTGACTTGAAAGCCTTCAAGAATTGGGCGAGGCAGATACTGCGAGGCTTGACGTATTTACATGGTCACAACCCTCCAATTATTCATAGGGATTTGAAATGTGATAATATATTTGTCAATGGAAATACGGGAGATGTTAAAATTGGAGATCTCGGTTTGGCGATTGTCATGCAGCAGCCTACTGCTCGAAGTGTTATTGGCACTCCAGAGTTCATGGCTCCAGAACTTTATGATGAGGAGTATAATGAGCTTGTTGATATCTATTCTTTTGGCATGTGCATGTTGGAAATTGTGACTTGTGAATACCCATATAGTGAGTGCAAAAATTCTGCCCAAATATTCAAGAAGGTTACTTCGGGTATAAAGCCTGCTTCCCTCGAAAAAGTGCTGGATCCCCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCCTTCCTTGCAACTGAAAGTCCCAAGGAGCATAGTTTTGAACCTTTAAGGTTATCGAATGAGCCGTTCAAATCTGAAAATCCTCCTGTGCTAGCTTCTCATTCAATGGACACAGACCATAACTGCAGCAAATTCTCAGGCTCAGTGGTCTCATCTGTCAAAAGCGATAACGACATTTCTCAGTTTTCAACTCTCGAACTTCAGAGGCTCACCGAGACTAATGAATTGACGCTAAAAGGGGACATGACTGATCATAATACAATTTCGTTCCATTTGCGCATTGCTGAACTAAGCGGTAAATCGAAGAATATCCATTTTGCCTTCTATCTCGACTCTGATACTTCATTGGCTATTGTTTTGGAGATGGTTGAACAACTCGAACTGTCAAATGAAGACGCGACTGTCATAGCGAAATTAATCGATGAATTAATTGCCAAGTTTGTTCCCGACTCGAAACCATGTCCAAACTATTATCACGGACAAAACACTACACACAGTTCTTCTGAAAGTGAAACTCAAGAGGACAAGACCTTCATTCCTCCTTTCTTCTCTGAGATGGTACTATCATCTCCCATGGTGGCTGGTGCAGGGAACAACCCGACGCATTTCACCAAGGTGGAAGATCAAGAAAACCTACAGTCGATCATTTCGTGTGCATCGCTCGACTTCAATCACTCAACTATCTCAGATTACAGCACAGGAAAAGGAACAATGGATTTCGATGCTGTTGGATCTATTTCGACGATGATCGATTTCGCAAACCCATCTCTAATCTCCTCCTGCTCAGGAATGTCGAAAGAAATGAGTTCGTCGAACTTTTCGAGCCTTTCTCTGGAAGAAAGAGATCACCAAGATGAACTGAAGATGGAGATTGATGCCATTGACTTACAGTACCATCAGTGCCTTTGTGAACTGTCAAGAATGAGAGAGGAAGCAATAGAAAGTGCGAAGAAGAGGTGGATGTCAAAGAAGAAAGCTCCTGCTTTTTGAAGCCTTCCTTTTTCCTTTGGATTTGCAAATTCTCTTTCTTTCTTGGCAAACTCTTGCTTGCAGAATGGAAGGTGTCTGGGAAACTGGTGGAGAGGTTATGATCTACACGAGAAAGGAGAATGAACCAATTTTGAACTTGATTCTGCTGCTCACGAGAGATATGAGGGAAGTTTTTAATACTACTTTTGTTCAACTATGGTGTTGCCTTTGCTGCTCTTTCTTTCTGCTATCGTCGTGTTATTGAGGGTTGGAACGATGATTTAGTCTCCGAACCATATTAGTATTTATTTCGTTTTATCTTTAAACTTTAAAGATTTTTATGTCGATAATACATAAATTAATAAATATTGGTTGATTTGATATGACGTGACAGATAGATGATATAACGAACAAATAAATTGGTTGATTTGATATGGGGC

Coding sequence (CDS)

ATGAGTTGCGGTTCGGATTTGATGTCTGGGAACTCCGAGGCTGATGATCGTGAGATTGTAGAGAAGGATCCAACAGGACGATACATTCGGTACGATGAGATTTTGGGTAAAGGGGCATTCAAGACAGTATACAGAGCATTCGACGAAGTCGGTGGAATCGAAGTTGCTTGGAGCCAAGTTGACATTGAAGATGTTTTGCAGTCACCAGAACAGCTTCAGAGATTATATTCTGAGGTTCATCTGCTGAAGTCTCTGAAACATGAAAATATAATCAAATTCTATTGCTATTGGGTTGATGATAAGAACAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAGGAAGCATAGGAAAGTTGACTTGAAAGCCTTCAAGAATTGGGCGAGGCAGATACTGCGAGGCTTGACGTATTTACATGGTCACAACCCTCCAATTATTCATAGGGATTTGAAATGTGATAATATATTTGTCAATGGAAATACGGGAGATGTTAAAATTGGAGATCTCGGTTTGGCGATTGTCATGCAGCAGCCTACTGCTCGAAGTGTTATTGGCACTCCAGAGTTCATGGCTCCAGAACTTTATGATGAGGAGTATAATGAGCTTGTTGATATCTATTCTTTTGGCATGTGCATGTTGGAAATTGTGACTTGTGAATACCCATATAGTGAGTGCAAAAATTCTGCCCAAATATTCAAGAAGGTTACTTCGGGTATAAAGCCTGCTTCCCTCGAAAAAGTGCTGGATCCCCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCCTTCCTTGCAACTGAAAGTCCCAAGGAGCATAGTTTTGAACCTTTAAGGTTATCGAATGAGCCGTTCAAATCTGAAAATCCTCCTGTGCTAGCTTCTCATTCAATGGACACAGACCATAACTGCAGCAAATTCTCAGGCTCAGTGGTCTCATCTGTCAAAAGCGATAACGACATTTCTCAGTTTTCAACTCTCGAACTTCAGAGGCTCACCGAGACTAATGAATTGACGCTAAAAGGGGACATGACTGATCATAATACAATTTCGTTCCATTTGCGCATTGCTGAACTAAGCGGTAAATCGAAGAATATCCATTTTGCCTTCTATCTCGACTCTGATACTTCATTGGCTATTGTTTTGGAGATGGTTGAACAACTCGAACTGTCAAATGAAGACGCGACTGTCATAGCGAAATTAATCGATGAATTAATTGCCAAGTTTGTTCCCGACTCGAAACCATGTCCAAACTATTATCACGGACAAAACACTACACACAGTTCTTCTGAAAGTGAAACTCAAGAGGACAAGACCTTCATTCCTCCTTTCTTCTCTGAGATGGTACTATCATCTCCCATGGTGGCTGGTGCAGGGAACAACCCGACGCATTTCACCAAGGTGGAAGATCAAGAAAACCTACAGTCGATCATTTCGTGTGCATCGCTCGACTTCAATCACTCAACTATCTCAGATTACAGCACAGGAAAAGGAACAATGGATTTCGATGCTGTTGGATCTATTTCGACGATGATCGATTTCGCAAACCCATCTCTAATCTCCTCCTGCTCAGGAATGTCGAAAGAAATGAGTTCGTCGAACTTTTCGAGCCTTTCTCTGGAAGAAAGAGATCACCAAGATGAACTGAAGATGGAGATTGATGCCATTGACTTACAGTACCATCAGTGCCTTTGTGAACTGTCAAGAATGAGAGAGGAAGCAATAGAAAGTGCGAAGAAGAGGTGGATGTCAAAGAAGAAAGCTCCTGCTTTTTGA

Protein sequence

MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLRLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELTLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIAKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAGNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAKKRWMSKKKAPAF
Homology
BLAST of CmoCh15G013760 vs. ExPASy Swiss-Prot
Match: Q944Q0 (Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=1 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 4.1e-166
Identity = 330/608 (54.28%), Postives = 414/608 (68.09%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           M+ GS  +   S  ++ +  EKDP+GRYIRYD++LG+GAFKTVY+AFDEV GIEVAW+ V
Sbjct: 1   MASGSGFLGQISSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
            IEDV+Q P QL+RLYSEVHLLK+LKHENIIK +  WVD+KNKTINMITELFTSGSLR Y
Sbjct: 61  SIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           R+KHRKVD KA KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTG+VKIGDLGLA
Sbjct: 121 RKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
            V+QQPTARSVIGTPEFMAPELY+EEYNELVDIYSFGMCMLE+VTCEYPY+EC+N AQI+
Sbjct: 181 TVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIY 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTS IKP SL KV DPQV+QFIEKCL+PAS R  A EL KDPFLA +  K+ +   L 
Sbjct: 241 KKVTSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSAL--LA 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
            S+   K   PP L    MD DHN +K       SV S+ D     T+ELQR+ E  E  
Sbjct: 301 SSSTSSKYVRPPQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFR 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           L+G+ +D  T S  LRIA+ SGK + +HFAFYL+SDT+ AI  EMVE+L L++++  VIA
Sbjct: 361 LRGERSDDVTASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
            +ID+ I + + D     + +H QN     S   T ED                    A 
Sbjct: 421 DMIDDFIMQLLSDR---TSSHHNQN-----SPRLTHEDHE------------------AA 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           N  T  +K E+    QS+ S  S D+       YS   G    +A     +M      S 
Sbjct: 481 NQQTVNSKDEEAAG-QSMKSDISADY----YFPYSANDGNAAMEAGRDAESM-----SSY 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           + SCS M      S   +LS+ + D+ ++LK E++ I+ Q++Q   +L +++E+AIE+AK
Sbjct: 541 LDSCSMM------STIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKLKEDAIENAK 557

Query: 601 KRWMSKKK 609
           ++W++KK+
Sbjct: 601 RKWITKKQ 557

BLAST of CmoCh15G013760 vs. ExPASy Swiss-Prot
Match: Q8RXE5 (Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 GN=WNK10 PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 2.0e-152
Identity = 310/590 (52.54%), Postives = 385/590 (65.25%), Query Frame = 0

Query: 15  DDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIEDVLQSPEQLQR 74
           ++ + V+KDPTGRYIRY+++LG+GAFKTVY+AFDEV GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 75  LYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRKHRKVDLKAFKN 134
           LYSEVHLL SLKH+NIIK +  WVDD NK+INMITELFTSGSL  YR+KHRKVD KA  N
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 135 WARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVMQQPTARSVIGT 194
           WARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG VKIGDLGLA VMQQPTARSVIGT
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 195 PEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKVTSGIKPASLEK 254
           PEFMAPELY+EEYNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSGIKP SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 255 VLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLRLSNEPFKSENPPVL 314
           V DPQVKQFIEKCL+PA  R  A ELLKD  LA +  K+ +      SN  FK   PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTL--TASSNTTFKPAMPPQC 301

Query: 315 ASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELTLKGDMTDHNTISFH 374
               MD ++     S S+ SS KS  + +   T+E+QR+ E+ E  L G+  D    S  
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 375 LRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIAKLIDELIAKFVPDS 434
           LRIA  SG+++ + F F L +DT+ A+  EMVE+L+LS+ + TVIA++IDELI K +  +
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMK-LKAN 421

Query: 435 KPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAGNNPTHFTKVEDQEN 494
           +  PN     N+ + S                                       +D+E 
Sbjct: 422 RSLPN----ANSVYQS---------------------------------------KDEEA 481

Query: 495 LQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSLISSCSGMSKEMSSS 554
            +S+ S  S D+ H   S+  +  G    +AV S+ +       S + SCS +S + S  
Sbjct: 482 GESMKSEISADYYHRVSSNEGSRLGCC-CEAVESLLS-------SFLDSCSMVSNKQS-- 521

Query: 555 NFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAKKRWM 605
                        ++LK E++ I+ QY+Q    L RM+EEAIE AK++WM
Sbjct: 542 -------------EDLKTELNVIESQYNQSCQRLLRMKEEAIEKAKRKWM 521

BLAST of CmoCh15G013760 vs. ExPASy Swiss-Prot
Match: Q6EU49 (Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK4 PE=2 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 7.5e-152
Identity = 315/620 (50.81%), Postives = 396/620 (63.87%), Query Frame = 0

Query: 13  EADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIEDVLQSPEQL 72
           + DD E VE DPT RYIRY+E+LG+GA KTVY+AFDEV GIEVAWSQV+I++V+QSP+ L
Sbjct: 9   KVDDAECVEVDPTRRYIRYNEVLGRGAMKTVYKAFDEVEGIEVAWSQVEIDEVMQSPDNL 68

Query: 73  QRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRKHRKVDLKAF 132
           +RLYSEVHLLKSLKHEN++KFY YWVDD+ KTIN+ITELFTSGSLRQYR+KH +VDLKA 
Sbjct: 69  ERLYSEVHLLKSLKHENVMKFYNYWVDDQKKTINVITELFTSGSLRQYRQKHPRVDLKAI 128

Query: 133 KNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVMQQPTARSVI 192
           KNWARQ+LRGL YLH H PPIIHRDLKCDNIFVNGN G+VKIGDLGLA VM  P A+SVI
Sbjct: 129 KNWARQVLRGLDYLHTHQPPIIHRDLKCDNIFVNGNHGEVKIGDLGLATVMLTPRAKSVI 188

Query: 193 GTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKVTSGIKPASL 252
           GTPEFMAPELYDE Y+ELVDIYSFGMCMLE+ T EYPYSEC N+AQIFKKV+ G+KPA+L
Sbjct: 189 GTPEFMAPELYDENYDELVDIYSFGMCMLEMFTLEYPYSECTNAAQIFKKVSKGVKPAAL 248

Query: 253 EKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLRLSNEPFKSENPP 312
            K+ + Q KQFI+KCLVPAS RL A ELL+DPFL +++         +  +   KS +  
Sbjct: 249 AKITNIQAKQFIDKCLVPASERLSAKELLQDPFLCSDN--SSVLVGTKFPSSLPKSVDVS 308

Query: 313 VLASHSMDTDHNCSKFSGSVVSSVKSDNDIS--QFSTLELQRLTETNELTLKGDMTDHNT 372
           + A H MD D N      S+ +S    ND+     S LE  R  +  EL L G+  D N+
Sbjct: 309 LEALH-MDVDTN-----ESMCTSTCKRNDLGGPHRSVLEFTRTNKNTELKLTGEKLDDNS 368

Query: 373 ISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIAKLIDELIAKF 432
           +S  LRIA+L G ++NIHF FYLDSDT++++  EMVEQLEL++ D T IA  ID LI   
Sbjct: 369 VSLVLRIADLCGHARNIHFLFYLDSDTAMSVAAEMVEQLELADCDVTFIADFIDLLIVNL 428

Query: 433 VPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAGNNPTHFTKVE 492
           VP  +             +SSES+  E +  I        L+   V   G   +      
Sbjct: 429 VPGQQ-----LMNDAVMSTSSESKMGESEHVITSQQHPSELTHDYVLVEGMMHSKEANAS 488

Query: 493 DQENLQSIISCASLDF-NHSTISDYSTGKGTMDFDAVGSISTMIDFANPSLISSCSGMSK 552
             + + S+++  +L   N S  SD S     +D  +       +D        +  G  K
Sbjct: 489 PSDYIDSLLNATNLGGPNSSEGSDISV---QLDGSSKSLSEYGVDEYRTLECGAYKGTDK 548

Query: 553 -------EMSSSNFSSLSLEERDHQDELKM---------------EIDAIDLQYHQCLCE 608
                     SSNF+   +++  H  EL +               E+  I+ QY Q   E
Sbjct: 549 LGCRHPLSNGSSNFAIFQMDQASHHSELVIGASVSITENRDVLNGELGLIEAQYEQWFRE 608

BLAST of CmoCh15G013760 vs. ExPASy Swiss-Prot
Match: Q8S8Y8 (Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN=WNK6 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.9e-142
Identity = 292/613 (47.63%), Postives = 372/613 (60.69%), Query Frame = 0

Query: 4   GSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIE 63
           G+D  S   E  D E++E DPT RYIRY E++GKGAFKTVY+AFDEV GIEVAW+QV I+
Sbjct: 3   GTDDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRID 62

Query: 64  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRK 123
           DVLQSP  L+RLYSEV LLKSLKH NII+FY  W+DDKNKT+N+ITELFTSGSLR YR+K
Sbjct: 63  DVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKK 122

Query: 124 HRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVM 183
           HRKV++KA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN G+VKIGDLGLA VM
Sbjct: 123 HRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 184 QQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKV 243
           +Q  A+SVIGTPEFMAPELYDE YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKV 242

Query: 244 TSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES------------- 303
           +SGIKPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL                 
Sbjct: 243 SSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIV 302

Query: 304 -PKEHSFEPLRLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLE 363
            PKE +F    L +E   +  P    S  +D D N           + + +D S    +E
Sbjct: 303 MPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNL---------PIVTFSDNSGSRCIE 362

Query: 364 LQRLTETNELTLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQL 423
           ++R    N   LKG+  D  ++S  LRI + +G+ +NIHF FY + DT+  +  EMVEQL
Sbjct: 363 VRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQL 422

Query: 424 ELSNEDATVIAKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEM 483
           EL++++ T IA+LID L+   +P  K      H     HS                    
Sbjct: 423 ELTDQNVTFIAELIDILLVNMIPTWKTDVTVDH---LIHSQLNQ---------------- 482

Query: 484 VLSSPMVAGAGNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSI 543
                      N+ +H  + + Q+  +++        +HS  SD                
Sbjct: 483 -----------NSRSHHNEAKPQKQEETVFHDTCELVSHSCNSDCPRS------------ 542

Query: 544 STMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELS 603
               D  +   + +  G  K        +      + ++ L+ E++ I+ +Y + + E++
Sbjct: 543 ----DEEDKQCVDATKGEDKSSIQEVEEATEPVSLEEEERLRQELEEIEAKYQEDMKEIA 560

BLAST of CmoCh15G013760 vs. ExPASy Swiss-Prot
Match: Q8LST2 (Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN=WNK7 PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 4.3e-139
Identity = 297/619 (47.98%), Postives = 381/619 (61.55%), Query Frame = 0

Query: 4   GSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIE 63
           GS+  S   E  D E++E DPT RYIRY E++GKGA KTV++ FDEV GIEVAW+QV I+
Sbjct: 3   GSEDASAIVEPPDPEVLEIDPTCRYIRYKEVIGKGASKTVFKGFDEVDGIEVAWNQVRID 62

Query: 64  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRK 123
           D+LQSP+ L+RLYSEV LLKSLKH+NII+FY  W+DDKNKT+N+ITELFTSGSLRQYR+K
Sbjct: 63  DLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYRKK 122

Query: 124 HRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVM 183
           HRKV++KA K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN G+VKIGDLGLA VM
Sbjct: 123 HRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 184 QQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKV 243
           +Q  A+SVIGTPEFMAPELYDE YNEL DIYSFGMCMLE+VT EYPY EC+NSAQI+KKV
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYKKV 242

Query: 244 TSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES------------- 303
           +SGIKPASL KV DP+V +FIEKCL+PAS RL A ELL D FL                 
Sbjct: 243 SSGIKPASLSKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPDIV 302

Query: 304 -PKEHSF-EPLRLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTL 363
            PKE SF E   +S  P  + N  +  S ++D D+N       V+SS  S       + +
Sbjct: 303 MPKEGSFGERCLMSEGPPNARNRTM--SMNLDEDNNLP----IVISSNNSGT-----NCI 362

Query: 364 ELQRLTETNELTLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQ 423
           E++R    N   LKG+  D N++S  LRI + +G+ +NIHF F+ + DT+  +  EMVEQ
Sbjct: 363 EVRRAKRGNFFVLKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQ 422

Query: 424 LELSNEDATVIAKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSE 483
           LEL++++   IA+LID L+   +      PN+       H     + Q  K         
Sbjct: 423 LELTDKNVKFIAELIDVLLVNLI------PNWKTDVAVDHLIHPQQNQSSK--------- 482

Query: 484 MVLSSPMVAGAGNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGS 543
                                ++ +N  S  S A    +HS  SDY              
Sbjct: 483 ---------------------DNHQNGAS--SQAGESISHSLSSDYCPRSD--------- 542

Query: 544 ISTMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCEL 603
                D ANP++ ++      E         SLEE +  + LK E++ I+ ++ + + E+
Sbjct: 543 -----DEANPTVAATTEDQEAEKPG------SLEEEEEDERLKEELEKIEERFREEMKEI 552

Query: 604 SRMREEAIESAKKRWMSKK 608
           +R REEA    K R+  KK
Sbjct: 603 TRKREEATMETKNRFFEKK 552

BLAST of CmoCh15G013760 vs. ExPASy TrEMBL
Match: A0A6J1EQ95 (serine/threonine-protein kinase WNK8-like OS=Cucurbita moschata OX=3662 GN=LOC111435638 PE=4 SV=1)

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 612/612 (100.00%), Postives = 612/612 (100.00%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV
Sbjct: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
           DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY
Sbjct: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA
Sbjct: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
           IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF
Sbjct: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR
Sbjct: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
           LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT
Sbjct: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA
Sbjct: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
           KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG
Sbjct: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL
Sbjct: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK
Sbjct: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600

Query: 601 KRWMSKKKAPAF 613
           KRWMSKKKAPAF
Sbjct: 601 KRWMSKKKAPAF 612

BLAST of CmoCh15G013760 vs. ExPASy TrEMBL
Match: A0A6J1I4B0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111469036 PE=4 SV=1)

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 605/612 (98.86%), Postives = 606/612 (99.02%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV
Sbjct: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
           DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY
Sbjct: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           R+KHRKVDLKAFKNWARQILRGLTYLH HNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA
Sbjct: 121 RKKHRKVDLKAFKNWARQILRGLTYLHTHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
           IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF
Sbjct: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR
Sbjct: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
           LSNE FKSENPP LASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT
Sbjct: 301 LSNEQFKSENPPGLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           LKGDMTDHNTISFHLRIAEL GKSKNIHFAFYLDSDTSLAI LEMVEQLELSNEDATVIA
Sbjct: 361 LKGDMTDHNTISFHLRIAELCGKSKNIHFAFYLDSDTSLAIALEMVEQLELSNEDATVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
           KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG
Sbjct: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           NNP HFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL
Sbjct: 481 NNPMHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK
Sbjct: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600

Query: 601 KRWMSKKKAPAF 613
           KRWMSKKKAPAF
Sbjct: 601 KRWMSKKKAPAF 612

BLAST of CmoCh15G013760 vs. ExPASy TrEMBL
Match: A0A5A7TLP6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001600 PE=4 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.4e-294
Identity = 530/632 (83.86%), Postives = 560/632 (88.61%), Query Frame = 0

Query: 1   MSCGSDL-MSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQ 60
           MSCGSDL MS NSEADDREIVEKDP+GRYIRYDEILGKGAFKTVY+AFDEVGGIEVAWSQ
Sbjct: 1   MSCGSDLIMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQ 60

Query: 61  VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQ 120
           VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFY YWVDDK+KTINMITELFTSGSLRQ
Sbjct: 61  VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQ 120

Query: 121 YRRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGL 180
           YR+KHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTG+VKIGDLGL
Sbjct: 121 YRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGL 180

Query: 181 AIVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQI 240
           AIVMQQPTARSVIGTPEFMAPELYDE+YNELVDIYSFGMCMLEIVTCEYPY+ECKNSAQI
Sbjct: 181 AIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQI 240

Query: 241 FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPL 300
           FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  ESPKEHS E  
Sbjct: 241 FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELS 300

Query: 301 RLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNEL 360
           R  NE FKS NPP+L SH MDTDH C+K SGSV SSVKS+N IS FST ELQRLTE NEL
Sbjct: 301 RSLNEQFKSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNEL 360

Query: 361 TLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVI 420
           TLKG+MTDHNT+SFHLRIAEL GKS+NIHFAFYLDSDTSLAI LEMVEQLELSNEDAT+I
Sbjct: 361 TLKGEMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATII 420

Query: 421 AKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGA 480
           AKLIDELIAKFVP  KPCPNY   +      S SE QE+K+FI PFFSE+VLSSPMV  A
Sbjct: 421 AKLIDELIAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAA 480

Query: 481 GNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGT------------------- 540
            NN T   KVEDQEN QSIISCAS+++ +ST+SDYS GKG+                   
Sbjct: 481 RNNLTGLAKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGT 540

Query: 541 --MDFDAVGS-ISTMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAI 600
             +D +AVGS ISTMIDFA PSLISSCSGMSKE+S S+FS+LS+EERDHQDELKMEIDAI
Sbjct: 541 IDLDAEAVGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAI 600

Query: 601 DLQYHQCLCELSRMREEAIESAKKRWMSKKKA 610
           DLQYHQCLCELSRMREEAIESAKKRWMSKKKA
Sbjct: 601 DLQYHQCLCELSRMREEAIESAKKRWMSKKKA 632

BLAST of CmoCh15G013760 vs. ExPASy TrEMBL
Match: A0A1S3AUL5 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103482852 PE=4 SV=1)

HSP 1 Score: 1020.0 bits (2636), Expect = 4.1e-294
Identity = 530/632 (83.86%), Postives = 559/632 (88.45%), Query Frame = 0

Query: 1   MSCGSDL-MSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQ 60
           MSCGSDL MS NSEADDREIVEKDP+GRYIRYDEILGKGAFKTVY+AFDEVGGIEVAWSQ
Sbjct: 1   MSCGSDLIMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQ 60

Query: 61  VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQ 120
           VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFY YWVDDK+KTINMITELFTSGSLRQ
Sbjct: 61  VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQ 120

Query: 121 YRRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGL 180
           YR+KHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTG+VKIGDLGL
Sbjct: 121 YRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGL 180

Query: 181 AIVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQI 240
           AIVMQQPTARSVIGTPEFMAPELYDE+YNELVDIYSFGMCMLEIVTCEYPY+ECKNSAQI
Sbjct: 181 AIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQI 240

Query: 241 FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPL 300
           FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  ESPKEHS E  
Sbjct: 241 FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELS 300

Query: 301 RLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNEL 360
           R  NE FKS NPP+L SH MDTDH C+K SGSV SSVKS+N IS FST ELQRLTE NEL
Sbjct: 301 RSLNEQFKSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNEL 360

Query: 361 TLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVI 420
           TLKG+MTDHNT+SFHLRIAEL GKS+NIHFAFYLDSDTSLAI LEMVEQLELSNEDAT+I
Sbjct: 361 TLKGEMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATII 420

Query: 421 AKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGA 480
           AKLIDELIAKFVP  KPCPNY   +      S SE QE+K+FI PFFSE+VLSSPMV  A
Sbjct: 421 AKLIDELIAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAA 480

Query: 481 GNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGT------------------- 540
            NN T   KVEDQEN QSIISCAS ++ +ST+SDYS GKG+                   
Sbjct: 481 RNNLTGLAKVEDQENQQSIISCASAEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGT 540

Query: 541 --MDFDAVGS-ISTMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAI 600
             +D +AVGS ISTMIDFA PSLISSCSGMSKE+S S+FS+LS+EERDHQDELKMEIDAI
Sbjct: 541 IDLDAEAVGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAI 600

Query: 601 DLQYHQCLCELSRMREEAIESAKKRWMSKKKA 610
           DLQYHQCLCELSRMREEAIESAKKRWMSKKKA
Sbjct: 601 DLQYHQCLCELSRMREEAIESAKKRWMSKKKA 632

BLAST of CmoCh15G013760 vs. ExPASy TrEMBL
Match: A0A0A0KQP1 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5G148620 PE=4 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.2e-293
Identity = 531/633 (83.89%), Postives = 562/633 (88.78%), Query Frame = 0

Query: 1   MSCGSDL-MSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQ 60
           +SCGSDL MSGNSEADDR IVEKDP+GRYIRYDEILGKGAFKTVY+AFDEVGGIEVAWSQ
Sbjct: 47  LSCGSDLIMSGNSEADDRGIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQ 106

Query: 61  VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQ 120
           VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFY YWVDDK+KTINMITELFTSGSLRQ
Sbjct: 107 VDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQ 166

Query: 121 YRRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGL 180
           YR+KHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTG+VKIGDLGL
Sbjct: 167 YRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGL 226

Query: 181 AIVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQI 240
           AIVMQQPTARSVIGTPEFMAPELYDE+YNELVDIYSFGMCMLEIVTCEYPY+ECKNSAQI
Sbjct: 227 AIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQI 286

Query: 241 FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPL 300
           FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPAS RLPASELLKDPFLA ESPK++S E  
Sbjct: 287 FKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSELS 346

Query: 301 RLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNEL 360
           R  NE FKS NPP+L SH M+TDHNC+K SGSV SSVKS+N IS FST ELQRLTE NEL
Sbjct: 347 RSLNEHFKSVNPPLLGSHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNEL 406

Query: 361 TLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVI 420
           TLKGDMTDHNT+SFHLRIAEL GKS+NIHFAFYLDSDTSLAI LEMVEQLELSNEDAT+I
Sbjct: 407 TLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATII 466

Query: 421 AKLIDELIAKFVPDSKPCPNY--YHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVA 480
           AKLIDELIAKFVP  KPCPNY     QNT HS    E QEDKTFI PFFSE+VLSSPMVA
Sbjct: 467 AKLIDELIAKFVPSWKPCPNYCEEQQQNTPHS---PEAQEDKTFISPFFSELVLSSPMVA 526

Query: 481 GAGNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGT----------------- 540
            A NN T   KVEDQEN QSIISCAS+++ +ST+SDYS GKG+                 
Sbjct: 527 AARNNLTGLAKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISS 586

Query: 541 ----MDFDAVGSISTMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDA 600
               +D +AVGS+ST IDFA PSLISSCS MSKE+S S+FS+LS+EERDHQDELKMEIDA
Sbjct: 587 GTIDLDAEAVGSLSTTIDFAKPSLISSCSEMSKELSLSSFSTLSMEERDHQDELKMEIDA 646

Query: 601 IDLQYHQCLCELSRMREEAIESAKKRWMSKKKA 610
           IDLQYHQCLCELSRMREEAIESAKKRWMSKKKA
Sbjct: 647 IDLQYHQCLCELSRMREEAIESAKKRWMSKKKA 676

BLAST of CmoCh15G013760 vs. NCBI nr
Match: XP_022928848.1 (serine/threonine-protein kinase WNK8-like [Cucurbita moschata])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 612/612 (100.00%), Postives = 612/612 (100.00%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV
Sbjct: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
           DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY
Sbjct: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA
Sbjct: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
           IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF
Sbjct: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR
Sbjct: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
           LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT
Sbjct: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA
Sbjct: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
           KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG
Sbjct: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL
Sbjct: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK
Sbjct: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600

Query: 601 KRWMSKKKAPAF 613
           KRWMSKKKAPAF
Sbjct: 601 KRWMSKKKAPAF 612

BLAST of CmoCh15G013760 vs. NCBI nr
Match: KAG6579581.1 (Serine/threonine-protein kinase WNK8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 607/612 (99.18%), Postives = 609/612 (99.51%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV
Sbjct: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
           DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY
Sbjct: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           R+KHRKVDLKAFKNWARQILRGLTYLH HNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA
Sbjct: 121 RKKHRKVDLKAFKNWARQILRGLTYLHTHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
           IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF
Sbjct: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR
Sbjct: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
           LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT
Sbjct: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           LKGDMTDHNTISFHLRIAEL GKSKNIHFAFYLDSDTSLAI LEMVEQLELSNEDATVIA
Sbjct: 361 LKGDMTDHNTISFHLRIAELCGKSKNIHFAFYLDSDTSLAIALEMVEQLELSNEDATVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
           KLI+ELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG
Sbjct: 421 KLINELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL
Sbjct: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK
Sbjct: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600

Query: 601 KRWMSKKKAPAF 613
           KRWMSKKKAPAF
Sbjct: 601 KRWMSKKKAPAF 612

BLAST of CmoCh15G013760 vs. NCBI nr
Match: XP_023520531.1 (serine/threonine-protein kinase WNK8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 606/612 (99.02%), Postives = 606/612 (99.02%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV
Sbjct: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
           DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY
Sbjct: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA
Sbjct: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
           IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF
Sbjct: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTSGIKPASLEKVLDPQVKQFIEKCLVPA MRLPASELLKDPFLATESPKEHSFEPLR
Sbjct: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPAPMRLPASELLKDPFLATESPKEHSFEPLR 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
           LSNE FKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT
Sbjct: 301 LSNEQFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           LKGDMTDHNTISFHLRIAEL GKSKNIHFAFYLDSDTSLAI LEMVEQLELSNEDATVIA
Sbjct: 361 LKGDMTDHNTISFHLRIAELCGKSKNIHFAFYLDSDTSLAIALEMVEQLELSNEDATVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
           KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMV GAG
Sbjct: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVGGAG 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           NN THFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL
Sbjct: 481 NNRTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK
Sbjct: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600

Query: 601 KRWMSKKKAPAF 613
           KRWMSKKKAPAF
Sbjct: 601 KRWMSKKKAPAF 612

BLAST of CmoCh15G013760 vs. NCBI nr
Match: XP_022970019.1 (serine/threonine-protein kinase WNK8-like [Cucurbita maxima])

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 605/612 (98.86%), Postives = 606/612 (99.02%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV
Sbjct: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
           DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY
Sbjct: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           R+KHRKVDLKAFKNWARQILRGLTYLH HNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA
Sbjct: 121 RKKHRKVDLKAFKNWARQILRGLTYLHTHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
           IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF
Sbjct: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR
Sbjct: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
           LSNE FKSENPP LASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT
Sbjct: 301 LSNEQFKSENPPGLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           LKGDMTDHNTISFHLRIAEL GKSKNIHFAFYLDSDTSLAI LEMVEQLELSNEDATVIA
Sbjct: 361 LKGDMTDHNTISFHLRIAELCGKSKNIHFAFYLDSDTSLAIALEMVEQLELSNEDATVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
           KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG
Sbjct: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           NNP HFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL
Sbjct: 481 NNPMHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK
Sbjct: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600

Query: 601 KRWMSKKKAPAF 613
           KRWMSKKKAPAF
Sbjct: 601 KRWMSKKKAPAF 612

BLAST of CmoCh15G013760 vs. NCBI nr
Match: KAG7017040.1 (Serine/threonine-protein kinase WNK8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 566/612 (92.48%), Postives = 569/612 (92.97%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV
Sbjct: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
           DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY
Sbjct: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           R+KHRKVDLKAFKNWARQILRGLTYLH HNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA
Sbjct: 121 RKKHRKVDLKAFKNWARQILRGLTYLHTHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
           IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPY+         
Sbjct: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYT--------- 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
                                          SMRLPASELLKDPFLATESPKEHSFEPLR
Sbjct: 241 -------------------------------SMRLPASELLKDPFLATESPKEHSFEPLR 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
           LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT
Sbjct: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           LKGDMTDHNTISFHLRIAEL GKSKNIHFAFYLDSDTSLAI LEMVEQLELSNEDATVIA
Sbjct: 361 LKGDMTDHNTISFHLRIAELCGKSKNIHFAFYLDSDTSLAIALEMVEQLELSNEDATVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
           KLI+ELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG
Sbjct: 421 KLINELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL
Sbjct: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK
Sbjct: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 572

Query: 601 KRWMSKKKAPAF 613
           KRWMSKKKAPAF
Sbjct: 601 KRWMSKKKAPAF 572

BLAST of CmoCh15G013760 vs. TAIR 10
Match: AT5G41990.1 (with no lysine (K) kinase 8 )

HSP 1 Score: 586.3 bits (1510), Expect = 2.9e-167
Identity = 330/608 (54.28%), Postives = 414/608 (68.09%), Query Frame = 0

Query: 1   MSCGSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQV 60
           M+ GS  +   S  ++ +  EKDP+GRYIRYD++LG+GAFKTVY+AFDEV GIEVAW+ V
Sbjct: 1   MASGSGFLGQISSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLV 60

Query: 61  DIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQY 120
            IEDV+Q P QL+RLYSEVHLLK+LKHENIIK +  WVD+KNKTINMITELFTSGSLR Y
Sbjct: 61  SIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVY 120

Query: 121 RRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLA 180
           R+KHRKVD KA KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTG+VKIGDLGLA
Sbjct: 121 RKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLA 180

Query: 181 IVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIF 240
            V+QQPTARSVIGTPEFMAPELY+EEYNELVDIYSFGMCMLE+VTCEYPY+EC+N AQI+
Sbjct: 181 TVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIY 240

Query: 241 KKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLR 300
           KKVTS IKP SL KV DPQV+QFIEKCL+PAS R  A EL KDPFLA +  K+ +   L 
Sbjct: 241 KKVTSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSAL--LA 300

Query: 301 LSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELT 360
            S+   K   PP L    MD DHN +K       SV S+ D     T+ELQR+ E  E  
Sbjct: 301 SSSTSSKYVRPPQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFR 360

Query: 361 LKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIA 420
           L+G+ +D  T S  LRIA+ SGK + +HFAFYL+SDT+ AI  EMVE+L L++++  VIA
Sbjct: 361 LRGERSDDVTASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIA 420

Query: 421 KLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAG 480
            +ID+ I + + D     + +H QN     S   T ED                    A 
Sbjct: 421 DMIDDFIMQLLSDR---TSSHHNQN-----SPRLTHEDHE------------------AA 480

Query: 481 NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSL 540
           N  T  +K E+    QS+ S  S D+       YS   G    +A     +M      S 
Sbjct: 481 NQQTVNSKDEEAAG-QSMKSDISADY----YFPYSANDGNAAMEAGRDAESM-----SSY 540

Query: 541 ISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAK 600
           + SCS M      S   +LS+ + D+ ++LK E++ I+ Q++Q   +L +++E+AIE+AK
Sbjct: 541 LDSCSMM------STIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKLKEDAIENAK 557

Query: 601 KRWMSKKK 609
           ++W++KK+
Sbjct: 601 RKWITKKQ 557

BLAST of CmoCh15G013760 vs. TAIR 10
Match: AT1G64630.1 (with no lysine (K) kinase 10 )

HSP 1 Score: 540.8 bits (1392), Expect = 1.4e-153
Identity = 310/590 (52.54%), Postives = 385/590 (65.25%), Query Frame = 0

Query: 15  DDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIEDVLQSPEQLQR 74
           ++ + V+KDPTGRYIRY+++LG+GAFKTVY+AFDEV GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 75  LYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRKHRKVDLKAFKN 134
           LYSEVHLL SLKH+NIIK +  WVDD NK+INMITELFTSGSL  YR+KHRKVD KA  N
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 135 WARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVMQQPTARSVIGT 194
           WARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG VKIGDLGLA VMQQPTARSVIGT
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 195 PEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKVTSGIKPASLEK 254
           PEFMAPELY+EEYNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSGIKP SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 255 VLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEPLRLSNEPFKSENPPVL 314
           V DPQVKQFIEKCL+PA  R  A ELLKD  LA +  K+ +      SN  FK   PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTL--TASSNTTFKPAMPPQC 301

Query: 315 ASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNELTLKGDMTDHNTISFH 374
               MD ++     S S+ SS KS  + +   T+E+QR+ E+ E  L G+  D    S  
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 375 LRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATVIAKLIDELIAKFVPDS 434
           LRIA  SG+++ + F F L +DT+ A+  EMVE+L+LS+ + TVIA++IDELI K +  +
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMK-LKAN 421

Query: 435 KPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAGAGNNPTHFTKVEDQEN 494
           +  PN     N+ + S                                       +D+E 
Sbjct: 422 RSLPN----ANSVYQS---------------------------------------KDEEA 481

Query: 495 LQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSISTMIDFANPSLISSCSGMSKEMSSS 554
            +S+ S  S D+ H   S+  +  G    +AV S+ +       S + SCS +S + S  
Sbjct: 482 GESMKSEISADYYHRVSSNEGSRLGCC-CEAVESLLS-------SFLDSCSMVSNKQS-- 521

Query: 555 NFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAKKRWM 605
                        ++LK E++ I+ QY+Q    L RM+EEAIE AK++WM
Sbjct: 542 -------------EDLKTELNVIESQYNQSCQRLLRMKEEAIEKAKRKWM 521

BLAST of CmoCh15G013760 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-143
Identity = 292/613 (47.63%), Postives = 372/613 (60.69%), Query Frame = 0

Query: 4   GSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIE 63
           G+D  S   E  D E++E DPT RYIRY E++GKGAFKTVY+AFDEV GIEVAW+QV I+
Sbjct: 3   GTDDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRID 62

Query: 64  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRK 123
           DVLQSP  L+RLYSEV LLKSLKH NII+FY  W+DDKNKT+N+ITELFTSGSLR YR+K
Sbjct: 63  DVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKK 122

Query: 124 HRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVM 183
           HRKV++KA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN G+VKIGDLGLA VM
Sbjct: 123 HRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 184 QQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKV 243
           +Q  A+SVIGTPEFMAPELYDE YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKV 242

Query: 244 TSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES------------- 303
           +SGIKPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL                 
Sbjct: 243 SSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIV 302

Query: 304 -PKEHSFEPLRLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLE 363
            PKE +F    L +E   +  P    S  +D D N           + + +D S    +E
Sbjct: 303 MPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNL---------PIVTFSDNSGSRCIE 362

Query: 364 LQRLTETNELTLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQL 423
           ++R    N   LKG+  D  ++S  LRI + +G+ +NIHF FY + DT+  +  EMVEQL
Sbjct: 363 VRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQL 422

Query: 424 ELSNEDATVIAKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEM 483
           EL++++ T IA+LID L+   +P  K      H     HS                    
Sbjct: 423 ELTDQNVTFIAELIDILLVNMIPTWKTDVTVDH---LIHSQLNQ---------------- 482

Query: 484 VLSSPMVAGAGNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSI 543
                      N+ +H  + + Q+  +++        +HS  SD                
Sbjct: 483 -----------NSRSHHNEAKPQKQEETVFHDTCELVSHSCNSDCPRS------------ 542

Query: 544 STMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELS 603
               D  +   + +  G  K        +      + ++ L+ E++ I+ +Y + + E++
Sbjct: 543 ----DEEDKQCVDATKGEDKSSIQEVEEATEPVSLEEEERLRQELEEIEAKYQEDMKEIA 560

BLAST of CmoCh15G013760 vs. TAIR 10
Match: AT3G18750.3 (with no lysine (K) kinase 6 )

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-143
Identity = 292/613 (47.63%), Postives = 372/613 (60.69%), Query Frame = 0

Query: 4   GSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIE 63
           G+D  S   E  D E++E DPT RYIRY E++GKGAFKTVY+AFDEV GIEVAW+QV I+
Sbjct: 3   GTDDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRID 62

Query: 64  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRK 123
           DVLQSP  L+RLYSEV LLKSLKH NII+FY  W+DDKNKT+N+ITELFTSGSLR YR+K
Sbjct: 63  DVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKK 122

Query: 124 HRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVM 183
           HRKV++KA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN G+VKIGDLGLA VM
Sbjct: 123 HRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 184 QQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKV 243
           +Q  A+SVIGTPEFMAPELYDE YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKV 242

Query: 244 TSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES------------- 303
           +SGIKPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL                 
Sbjct: 243 SSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIV 302

Query: 304 -PKEHSFEPLRLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLE 363
            PKE +F    L +E   +  P    S  +D D N           + + +D S    +E
Sbjct: 303 MPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNL---------PIVTFSDNSGSRCIE 362

Query: 364 LQRLTETNELTLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQL 423
           ++R    N   LKG+  D  ++S  LRI + +G+ +NIHF FY + DT+  +  EMVEQL
Sbjct: 363 VRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQL 422

Query: 424 ELSNEDATVIAKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEM 483
           EL++++ T IA+LID L+   +P  K      H     HS                    
Sbjct: 423 ELTDQNVTFIAELIDILLVNMIPTWKTDVTVDH---LIHSQLNQ---------------- 482

Query: 484 VLSSPMVAGAGNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGSI 543
                      N+ +H  + + Q+  +++        +HS  SD                
Sbjct: 483 -----------NSRSHHNEAKPQKQEETVFHDTCELVSHSCNSDCPRS------------ 542

Query: 544 STMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCELS 603
               D  +   + +  G  K        +      + ++ L+ E++ I+ +Y + + E++
Sbjct: 543 ----DEEDKQCVDATKGEDKSSIQEVEEATEPVSLEEEERLRQELEEIEAKYQEDMKEIA 560

BLAST of CmoCh15G013760 vs. TAIR 10
Match: AT1G49160.2 (Protein kinase superfamily protein )

HSP 1 Score: 496.5 bits (1277), Expect = 3.0e-140
Identity = 297/619 (47.98%), Postives = 381/619 (61.55%), Query Frame = 0

Query: 4   GSDLMSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTVYRAFDEVGGIEVAWSQVDIE 63
           GS+  S   E  D E++E DPT RYIRY E++GKGA KTV++ FDEV GIEVAW+QV I+
Sbjct: 3   GSEDASAIVEPPDPEVLEIDPTCRYIRYKEVIGKGASKTVFKGFDEVDGIEVAWNQVRID 62

Query: 64  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLRQYRRK 123
           D+LQSP+ L+RLYSEV LLKSLKH+NII+FY  W+DDKNKT+N+ITELFTSGSLRQYR+K
Sbjct: 63  DLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYRKK 122

Query: 124 HRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLGLAIVM 183
           HRKV++KA K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN G+VKIGDLGLA VM
Sbjct: 123 HRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 184 QQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQIFKKV 243
           +Q  A+SVIGTPEFMAPELYDE YNEL DIYSFGMCMLE+VT EYPY EC+NSAQI+KKV
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYKKV 242

Query: 244 TSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES------------- 303
           +SGIKPASL KV DP+V +FIEKCL+PAS RL A ELL D FL                 
Sbjct: 243 SSGIKPASLSKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPDIV 302

Query: 304 -PKEHSF-EPLRLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTL 363
            PKE SF E   +S  P  + N  +  S ++D D+N       V+SS  S       + +
Sbjct: 303 MPKEGSFGERCLMSEGPPNARNRTM--SMNLDEDNNLP----IVISSNNSGT-----NCI 362

Query: 364 ELQRLTETNELTLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQ 423
           E++R    N   LKG+  D N++S  LRI + +G+ +NIHF F+ + DT+  +  EMVEQ
Sbjct: 363 EVRRAKRGNFFVLKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQ 422

Query: 424 LELSNEDATVIAKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSE 483
           LEL++++   IA+LID L+   +      PN+       H     + Q  K         
Sbjct: 423 LELTDKNVKFIAELIDVLLVNLI------PNWKTDVAVDHLIHPQQNQSSK--------- 482

Query: 484 MVLSSPMVAGAGNNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGTMDFDAVGS 543
                                ++ +N  S  S A    +HS  SDY              
Sbjct: 483 ---------------------DNHQNGAS--SQAGESISHSLSSDYCPRSD--------- 542

Query: 544 ISTMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDAIDLQYHQCLCEL 603
                D ANP++ ++      E         SLEE +  + LK E++ I+ ++ + + E+
Sbjct: 543 -----DEANPTVAATTEDQEAEKPG------SLEEEEEDERLKEELEKIEERFREEMKEI 552

Query: 604 SRMREEAIESAKKRWMSKK 608
           +R REEA    K R+  KK
Sbjct: 603 TRKREEATMETKNRFFEKK 552

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944Q04.1e-16654.28Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=... [more]
Q8RXE52.0e-15252.54Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q6EU497.5e-15250.81Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX... [more]
Q8S8Y81.9e-14247.63Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8LST24.3e-13947.98Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1EQ950.0e+00100.00serine/threonine-protein kinase WNK8-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1I4B00.0e+0098.86Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5A7TLP62.4e-29483.86Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AUL54.1e-29483.86Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KQP11.2e-29383.89Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5... [more]
Match NameE-valueIdentityDescription
XP_022928848.10.0e+00100.00serine/threonine-protein kinase WNK8-like [Cucurbita moschata][more]
KAG6579581.10.0e+0099.18Serine/threonine-protein kinase WNK8, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023520531.10.0e+0099.02serine/threonine-protein kinase WNK8-like [Cucurbita pepo subsp. pepo][more]
XP_022970019.10.0e+0098.86serine/threonine-protein kinase WNK8-like [Cucurbita maxima][more]
KAG7017040.10.0e+0092.48Serine/threonine-protein kinase WNK8 [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
AT5G41990.12.9e-16754.28with no lysine (K) kinase 8 [more]
AT1G64630.11.4e-15352.54with no lysine (K) kinase 10 [more]
AT3G18750.11.3e-14347.63with no lysine (K) kinase 6 [more]
AT3G18750.31.3e-14347.63with no lysine (K) kinase 6 [more]
AT1G49160.23.0e-14047.98Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 29..286
e-value: 1.2E-60
score: 217.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 32..286
e-value: 2.5E-50
score: 171.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 29..286
score: 41.120766
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 112..292
e-value: 1.5E-51
score: 176.5
NoneNo IPR availableGENE3D3.10.20.90coord: 369..463
e-value: 2.0E-5
score: 26.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 12..110
e-value: 1.1E-36
score: 126.7
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 27..235
e-value: 7.8E-26
score: 88.5
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 33..224
e-value: 3.5E-12
score: 44.0
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 29..268
e-value: 1.1E-11
score: 42.1
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 16..288
e-value: 1.3E-25
score: 87.8
NoneNo IPR availablePANTHERPTHR13902:SF139WNK KINASEcoord: 11..607
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 11..607
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 27..286
e-value: 6.2799E-170
score: 481.725
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 153..165
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 30..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G013760.1CmoCh15G013760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity