CmoCh15G010730 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh15G010730
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	cytochrome b5-like
Location	Cmo_Chr15: 6968038 .. 6970578 (-)
RNA-Seq Expression	CmoCh15G010730
Synteny	CmoCh15G010730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACTAAATTAAAAGAAAAATAAATGGATTCATTTCGTATTTAGGCGATAATCAAAGAAAAAGAAAGGAAGAGATTATAATTTTTTTCTTTTGGTCCAGAGAGACAATCAAATTAGATACGTCAAGGAACAAAACACGATCACTGATCGTTAGGGCAGAAAAGTTTGAATTACATTACCCACTTGTTTTGAAGTTTCCGAGGGATTTTTCCATTGGGAAATCGCCCATGGATTCAAAATCCGCACTACCTTTTCACCTTTTGACTTCGTAATTTACTTCTTGTAATTCGAGGGGATTTTTGCCGCACTTTCCGTTGTTCTTTCATTGTTATTATAAAATGATTTTTGATGCCGAATCAGGAACTAATCTGCACTCGGGCTTTGCCGCAAACAACAGGGATCCGAACAATTCGTTGCCTCGTCAACCCTTTTCCCTCTGTAAGTGTGCTTGTTTCTTAGCGCCGACCCACTTCGGTTTCTTATCTAGACTTGGTTTCTGTATTTTCGTTTCGTTTTTTTATATGAAGTTTCAAGTTCTTGGCGTCTGCTCTTTGTAGGGTTTGTGAATCTCGATTGTTTCTTGATTTTTGTAGTGTTAAATGGCTTCGGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTGGATTGGATCTCTCTTAGTTGATTGTTCGTATCATTTTTATTTAGCACAGATTATCAGTTCTGCTATAATTTCTGGATATTTCTCTTTTGATTGGTAAGAAAAGCATATGGATATGGCTAATGTTCTTAACTCTTGCTAACTCAGTGTGCCCATTGGATATCTGCTACAAGAGTCTGATATAGATGCCTTAGCTTTGGGGTTTCATTGTTTTGTATGCACTTTCACACATTTCTGTTTCAGATTCTCAAATTCAAATATCTTCTCCCGTTTTCTTCATTGCTTATGTTCATACGCGAGATTCCATATATGCTTTTAATATAGAAGATAATTTAGTCTCATTTCTTTCTGTTCTGTTTCTATTATCTGGATTTGTTTGTATGACTGAATTACATGTTTATGTAGTGTCTTAATGTTTCTGTGCCATAGTCATCTCAAATGGGGAAATAATGGTGGAGAGCTGTTAGGATGTTTATATTATAGTGTGTGAAAGGCTATATAAAGGACTGAAATTGAGCAAATGATTTATTGTGAGATTCCTCGTTGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTACCTAATAGATGCGTTTTGAAATCGCGAGGCTGACGGCGATACGTAATGGGACGAAGTGGACAATATCAGTTAGCGGTGGACTTAGGCTGTTACAAATGGTATCAAAGCCAGACACCGAGCGGTGTGCCAACAAGGACGTTGGGCTCTCAAGAGGGTGGATTGTGAGATCCCATGTTGGTTAGAGAGGAGAACGAAAGATTTCTTATAAGAGTGTGGAAACCTCTCCCTAACAGACGCGTTTTAAAATCTTGAGGTTGACAGTGATCCATAATGAGCCAAAGCGGACAATATGAGTTAGTGGTGGGCTTGGACTATTACAAATGATATCAAAGCCAGACACTGAGGAGTGTGCTAGCGAGGACACTGGCCCCCAAAGGGGGTGGAATTTGAGATCCCACATTGGTTGGAGAGGAGGACGAAACATTCCTTATAAGGGTGTGAAAACCTCTCCCTAACAGACACGTTTTAAAATCGTGAGGCCAAAACAGACAATATCAGTTAGCGAGTTAGCGGTGGGCAGATAAGATATCTTTTCTGATGTTTGATTGCCTGTGGCCTTGGCTTTAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGGTAATCCTCTGAGTTCCCTTACAACAAACACTAGATTTCGTTTCCTTGAGTTCTCGTGCAAATGACGATTGCGATGATACGATAACAGTTACTGATTCGAATGTTTTGCAGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATTGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGAATAAGTGAATGAATAAAGCCTCTAGAGGGTAAACTGCTTATTCATAATGATTATGTTTGGTTTGTGTTTTCATGTGGAGGGTTGGTCGTAGACTAGAGTTGTCTGGATCATATCTTATATTCTACTTAATAATAAGTTAGCTAAGTGCTATGTTCTTGTTTTCTTTTTCAATTTTGCATCTCGAAGTTGTATCAAAATGCGGATAACTATTTGTAACCTTAAAACGATAAGGAATCACTTCAAGGGAAGGTGAGATTTTGATTAC

mRNA sequence

AAACTAAATTAAAAGAAAAATAAATGGATTCATTTCGTATTTAGGCGATAATCAAAGAAAAAGAAAGGAAGAGATTATAATTTTTTTCTTTTGGTCCAGAGAGACAATCAAATTAGATACGTCAAGGAACAAAACACGATCACTGATCGTTAGGGCAGAAAAGTTTGAATTACATTACCCACTTGTTTTGAAGTTTCCGAGGGATTTTTCCATTGGGAAATCGCCCATGGATTCAAAATCCGCACTACCTTTTCACCTTTTGACTTCGTAATTTACTTCTTGTAATTCGAGGGGATTTTTGCCGCACTTTCCGTTGTTCTTTCATTGTTATTATAAAATGATTTTTGATGCCGAATCAGGAACTAATCTGCACTCGGGCTTTGCCGCAAACAACAGGGATCCGAACAATTCGTTGCCTCGTCAACCCTTTTCCCTCTTGTTAAATGGCTTCGGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATTGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGAATAAGTGAATGAATAAAGCCTCTAGAGGGTAAACTGCTTATTCATAATGATTATGTTTGGTTTGTGTTTTCATGTGGAGGGTTGGTCGTAGACTAGAGTTGTCTGGATCATATCTTATATTCTACTTAATAATAAGTTAGCTAAGTGCTATGTTCTTGTTTTCTTTTTCAATTTTGCATCTCGAAGTTGTATCAAAATGCGGATAACTATTTGTAACCTTAAAACGATAAGGAATCACTTCAAGGGAAGGTGAGATTTTGATTAC

Coding sequence (CDS)

ATGGCTTCGGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATTGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGA

Protein sequence

MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFILGLAFAVRHYTKNE

Homology

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BLAST of CmoCh15G010730 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 7.6e-57
Identity = 102/134 (76.12%), Postives = 116/134 (86.57%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKT EF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of CmoCh15G010730 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 5.5e-55
Identity = 101/134 (75.37%), Postives = 114/134 (85.07%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MAS+ KV  FEEV++HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++TGKDATNDFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AR+MM+KYYIGEID STVP  + Y+   Q  YN DKT EF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VR YTK E
Sbjct: 121 LGLALVVRQYTKKE 134

[Close]

BLAST of CmoCh15G010730 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-48
Identity = 86/132 (65.15%), Postives = 106/132 (80.30%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  + KV    EV++HN  KDCWLVISGKVYDVT F++DHPGGDEVLL+ATGKDAT+DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHS SAR M+D+YY+G+ID +T+P +  Y P  Q  YN DKT+EFV+K+LQ LVP+ I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAFAVRHYTK 133
           LG+AF +R YTK
Sbjct: 121 LGVAFGIRFYTK 132

[Close]

BLAST of CmoCh15G010730 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 1.7e-48
Identity = 87/129 (67.44%), Postives = 107/129 (82.95%), Query Frame = 0


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHS 65
           KV+  EEVAKHN   DCWL+I GKVY+V+ F+EDHPGGD+VLL++TGKDAT+DFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  DSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFILGLAF 125
            +AR MMD+YY+G+ID ST+P R  Y+P +Q  YN DKT EF+IKILQ LVP+ ILGLA 
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 AVRHYTKNE 135
           A+R YTK+E
Sbjct: 127 AIRIYTKSE 135

[Close]

BLAST of CmoCh15G010730 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 8.4e-48
Identity = 83/133 (62.41%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLL++TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P +  Y P +Q  YN DKT+EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of CmoCh15G010730 vs. ExPASy TrEMBL
Match: A0A6J1E3B3 (cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 6.7e-72
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. ExPASy TrEMBL
Match: A0A6J1IA07 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111472970 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.2e-70
Identity = 130/134 (97.01%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVH FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE
Sbjct: 1   MASDPKVHPFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEI+PSTVPLRK+YIP QQTQYNPDKTAEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIEPSTVPLRKVYIPPQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. ExPASy TrEMBL
Match: A0A6J1CBE4 (cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-68
Identity = 127/134 (94.78%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+++YIP+QQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. ExPASy TrEMBL
Match: A0A6J1K8E3 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111491071 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.5e-68
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP +KIYIPSQQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. ExPASy TrEMBL
Match: A0A0A0KN46 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 5.8e-68
Identity = 126/134 (94.03%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+KI+IPSQQ+Q+NPDKT EFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. NCBI nr
Match: XP_022922141.1 (cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_023549751.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG6579306.1 Cytochrome b5 isoform E, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016804.1 Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 279.6 bits (714), Expect = 1.4e-71
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. NCBI nr
Match: XP_022974337.1 (cytochrome b5-like [Cucurbita maxima] >XP_022974338.1 cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 130/134 (97.01%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVH FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE
Sbjct: 1   MASDPKVHPFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEI+PSTVPLRK+YIP QQTQYNPDKTAEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIEPSTVPLRKVYIPPQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. NCBI nr
Match: XP_022137808.1 (cytochrome b5 [Momordica charantia])

HSP 1 Score: 268.9 bits (686), Expect = 2.4e-68
Identity = 127/134 (94.78%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+++YIP+QQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. NCBI nr
Match: XP_022995583.1 (cytochrome b5-like [Cucurbita maxima] >XP_023532432.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG6605804.1 Cytochrome b5 isoform E, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035767.1 Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 268.5 bits (685), Expect = 3.2e-68
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP +KIYIPSQQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. NCBI nr
Match: XP_011654591.1 (cytochrome b5 [Cucumis sativus] >KGN49837.1 hypothetical protein Csa_000069 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.2e-67
Identity = 126/134 (94.03%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+KI+IPSQQ+Q+NPDKT EFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmoCh15G010730 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 221.1 bits (562), Expect = 5.4e-58
Identity = 102/134 (76.12%), Postives = 116/134 (86.57%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKT EF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of CmoCh15G010730 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 191.0 bits (484), Expect = 6.0e-49
Identity = 83/133 (62.41%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLL++TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P +  Y P +Q  YN DKT+EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of CmoCh15G010730 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 178.3 bits (451), Expect = 4.0e-45
Identity = 78/136 (57.35%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  D KV    EV++H+  KDCW+VI GKVYDVT F++DHPGGDEV+L +TGKDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQ--YNPDKTAEFVIKILQILVPI 120
           DVGHS +A+ M+D+YY+G+ID +TVP++  ++P   T+     DK+++FVIK+LQ LVP+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 FILGLAFAVRHYTKNE 135
            ILGLAF +R+YTK +
Sbjct: 121 LILGLAFGIRYYTKTK 136

[Close]

BLAST of CmoCh15G010730 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 138.7 bits (348), Expect = 3.5e-33
Identity = 66/123 (53.66%), Postives = 88/123 (71.54%), Query Frame = 0


Query: 10  FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHSDSAR 69
           F +VAKH    DCW++I GKVYD++ FM++HPGGD VLLA TGKDA+ DFEDV HS  A+
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTA--EFVIKILQILVPIFILGLAFAV 129
           E+M KY IG++D STVP+ + YIP  + +    +T   E   K+L  L+P+ ILG+AFA+
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFAL 126

Query: 130 RHY 131
           R Y
Sbjct: 127 RFY 129

[Close]

BLAST of CmoCh15G010730 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 127.5 bits (319), Expect = 8.1e-30
Identity = 63/127 (49.61%), Postives = 88/127 (69.29%), Query Frame = 0


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHS 65
           K++  EE A HNK  DCW+VI GKVYDV+ +M++HPGGD+VLLA  GKDAT+DFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQI-----LVPIFI 125
             ARE+M+KY+IGE+D S++P     IP  +  Y  D+  + V K+  +     +VP+ I
Sbjct: 66  KDARELMEKYFIGELDESSLP----EIPELKI-YKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAFAV 128
           + ++ AV
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:

BLAST of CmoCh15G010730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	7.6e-57	76.12	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	5.5e-55	75.37	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	1.3e-48	65.15	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	1.7e-48	67.44	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
O48845	8.4e-48	62.41	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of CmoCh15G010730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E3B3	6.7e-72	100.00	cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3...	[more]
A0A6J1IA07	6.2e-70	97.01	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111472970 PE=3 SV=1	[more]
A0A6J1CBE4	1.2e-68	94.78	cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1	[more]
A0A6J1K8E3	1.5e-68	95.52	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111491071 PE=3 SV=1	[more]
A0A0A0KN46	5.8e-68	94.03	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]

BLAST of CmoCh15G010730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922141.1	1.4e-71	100.00	cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5...	[more]
XP_022974337.1	1.3e-69	97.01	cytochrome b5-like [Cucurbita maxima] >XP_022974338.1 cytochrome b5-like [Cucurb...	[more]
XP_022137808.1	2.4e-68	94.78	cytochrome b5 [Momordica charantia]	[more]
XP_022995583.1	3.2e-68	95.52	cytochrome b5-like [Cucurbita maxima] >XP_023532432.1 cytochrome b5-like [Cucurb...	[more]
XP_011654591.1	1.2e-67	94.03	cytochrome b5 [Cucumis sativus] >KGN49837.1 hypothetical protein Csa_000069 [Cuc...	[more]

BLAST of CmoCh15G010730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	5.4e-58	76.12	cytochrome B5 isoform E	[more]
AT2G32720.1	6.0e-49	62.41	cytochrome B5 isoform B	[more]
AT5G48810.1	4.0e-45	57.35	cytochrome B5 isoform D	[more]
AT2G46650.1	3.5e-33	53.66	cytochrome B5 isoform C	[more]
AT1G26340.1	8.1e-30	49.61	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 60.74 coord: 40..54 score: 53.23 coord: 55..62 score: 45.27 coord: 68..80 score: 44.99
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 1.5E-30 score: 117.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 3.4E-27 score: 94.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29.402718
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..86 e-value: 1.1E-36 score: 126.6
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..87
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..132
None	No IPR available	PANTHER	PTHR19359:SF103	CYTOCHROME B5-LIKE	coord: 1..132
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh15G010730.1	CmoCh15G010730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035672	oligopeptide transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0035673	oligopeptide transmembrane transporter activity