CmoCh15G010260 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh15G010260
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Polcalcin Jun o
Location	Cmo_Chr15: 6071915 .. 6072487 (-)
RNA-Seq Expression	CmoCh15G010260
Synteny	CmoCh15G010260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATTCAAATCTCTCTTCTCCCTAAAGATCAAGAAATCCGATGCCTCCACCGACTCACCGCTTCCATCTACGCCGGTTCCCGTTCTCGGATCTCGGACGCCGTCTTTCAATTCCCGAGCGCAACTCGCGGACCTCCAGCAAGTATTCAAGAAATTCGATGTCAACGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGCGGAGCCTCGGCCAGCCGGCCACCGAGGAGGAGATTCAGAGCATGATCGAGGAGGTGGATGCTGACGGCGACGGCTACATCGACCTCCACGAGTTCATCGAGCTCAACACCAAGGACGTGGATTCCGACGAGGTGCTCGCGAATCTGAAGGACGCGTTCTCTGTTTACGACATCGACGGAAACGGATCCATTACGGCGGAGGAATTGCAGGAGGTTTTGAAGAGCTTGGGCGACGAATGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAAAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTGATGATGATGGTGAGCGGCTCTCGATCCGGCTTCAATGCTTAA

mRNA sequence

ATGGGATTCAAATCTCTCTTCTCCCTAAAGATCAAGAAATCCGATGCCTCCACCGACTCACCGCTTCCATCTACGCCGGTTCCCGTTCTCGGATCTCGGACGCCGTCTTTCAATTCCCGAGCGCAACTCGCGGACCTCCAGCAAGTATTCAAGAAATTCGATGTCAACGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGCGGAGCCTCGGCCAGCCGGCCACCGAGGAGGAGATTCAGAGCATGATCGAGGAGGTGGATGCTGACGGCGACGGCTACATCGACCTCCACGAGTTCATCGAGCTCAACACCAAGGACGTGGATTCCGACGAGGTGCTCGCGAATCTGAAGGACGCGTTCTCTGTTTACGACATCGACGGAAACGGATCCATTACGGCGGAGGAATTGCAGGAGGTTTTGAAGAGCTTGGGCGACGAATGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAAAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTGATGATGATGGTGAGCGGCTCTCGATCCGGCTTCAATGCTTAA

Coding sequence (CDS)

ATGGGATTCAAATCTCTCTTCTCCCTAAAGATCAAGAAATCCGATGCCTCCACCGACTCACCGCTTCCATCTACGCCGGTTCCCGTTCTCGGATCTCGGACGCCGTCTTTCAATTCCCGAGCGCAACTCGCGGACCTCCAGCAAGTATTCAAGAAATTCGATGTCAACGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGCGGAGCCTCGGCCAGCCGGCCACCGAGGAGGAGATTCAGAGCATGATCGAGGAGGTGGATGCTGACGGCGACGGCTACATCGACCTCCACGAGTTCATCGAGCTCAACACCAAGGACGTGGATTCCGACGAGGTGCTCGCGAATCTGAAGGACGCGTTCTCTGTTTACGACATCGACGGAAACGGATCCATTACGGCGGAGGAATTGCAGGAGGTTTTGAAGAGCTTGGGCGACGAATGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAAAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTGATGATGATGGTGAGCGGCTCTCGATCCGGCTTCAATGCTTAA

Protein sequence

MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMMVSGSRSGFNA

Homology

[Close]

BLAST of CmoCh15G010260 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.9e-51
Identity = 96/148 (64.86%), Postives = 119/148 (80.41%), Query Frame = 0


Query: 38  NSRAQLADLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYI 97
           + + ++ +L+ VFKKFDVNGDGKI S ELG+IM SLG    EEE++  I E+D  GDGYI
Sbjct: 30  SGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYI 89

Query: 98  DLHEFIELNTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRK 157
           +  EF+ELNTK +D ++VL NLKDAFSVYDIDGNGSI+AEEL EVL+SLGDECS+A+CRK
Sbjct: 90  NFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRK 149

Query: 158 MITGVDKNGDGMISFDEFKVMMMVSGSR 186
           MI GVDK+GDG I F+EFK+MM +   R
Sbjct: 150 MIGGVDKDGDGTIDFEEFKIMMTMGSRR 177

[Close]

BLAST of CmoCh15G010260 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.1e-36
Identity = 83/174 (47.70%), Postives = 114/174 (65.52%), Query Frame = 0


Query: 12  KKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGKICSSELGSIMR 71
           ++   S   PLP    P  G  +P+        ++++VF+KFD NGDG+I  SELG++  
Sbjct: 11  RRRSGSKSPPLPQAD-PASGGGSPAPTPE---EEMERVFRKFDANGDGRISRSELGALFE 70

Query: 72  SLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELN-TKDVDSDEVLANLKDAFSVYDIDG 131
           SLG  AT++E+  M+ E DADGDG+I L EF  LN T   D+  V  +L+ AF V+D DG
Sbjct: 71  SLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADG 130

Query: 132 NGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMMVSGS 185
           NG+I+A EL  VL  LG++ ++  CR+MI GVD+NGDG+ISF+EFKVMM   GS
Sbjct: 131 NGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGGS 180

[Close]

BLAST of CmoCh15G010260 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 5.8e-34
Identity = 71/135 (52.59%), Postives = 98/135 (72.59%), Query Frame = 0


Query: 45  DLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIE 104
           +L++VFKKFD NGDGKI  SEL  I+RSLG    E E+++M+EE DADGDGY+ L EF++
Sbjct: 26  ELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEFVD 85

Query: 105 LNTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDK 164
           LN K       + +LK+AF V+D D NGSI+A EL   L+S+G+ C++ + + +I  VDK
Sbjct: 86  LNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNVDK 145

Query: 165 NGDGMISFDEFKVMM 180
           NGDG+IS +EF+ MM
Sbjct: 146 NGDGLISVEEFQTMM 156

[Close]

BLAST of CmoCh15G010260 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.7e-33
Identity = 68/135 (50.37%), Postives = 97/135 (71.85%), Query Frame = 0


Query: 45  DLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIE 104
           +L++VFKKFD NGDGKI  SEL  I+RS+G    E E+++M+EE D DGDGY+ L EF++
Sbjct: 26  ELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEFVD 85

Query: 105 LNTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDK 164
           LN K       + +LK+AF V+D D NG+I+  EL E LKS+G+ C++ + + +I  VDK
Sbjct: 86  LNIKGA----TVKDLKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHNVDK 145

Query: 165 NGDGMISFDEFKVMM 180
           NGDG+I+ +EF+ MM
Sbjct: 146 NGDGLINVEEFQTMM 156

[Close]

BLAST of CmoCh15G010260 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.4e-33
Identity = 68/155 (43.87%), Postives = 108/155 (69.68%), Query Frame = 0


Query: 32  SRTPSFNSRAQLADLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDA 91
           S+  S N    + D+++VF++FD N DGKI   EL  ++ +L   A++EE ++M++E D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 92  DGDGYIDLHEFIEL--NTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDE 151
           DG+G+IDL EF+ L   +    ++  + +LK+AF +YD+D NG I+A EL  V+K+LG++
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 152 CSLADCRKMITGVDKNGDGMISFDEFKVMMMVSGS 185
           CS+ DC++MI  VD +GDG + F+EFK MMM++GS
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMMINGS 156

[Close]

BLAST of CmoCh15G010260 vs. ExPASy TrEMBL
Match: A0A6J1FFM7 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111445291 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.1e-99
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180

Query: 181 VSGSRSGFNA 191
           VSGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

[Close]

BLAST of CmoCh15G010260 vs. ExPASy TrEMBL
Match: A0A6J1IDH5 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111472087 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 8.2e-92
Identity = 180/191 (94.24%), Postives = 184/191 (96.34%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSF--NSRAQLADLQQVFKKFDVNGD 60
           MGFKSLFSLKIKKSDASTDSPL ST VPVLGSRTPSF  NSR QLA+LQQVFKKFDVNGD
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPVLGSRTPSFNSNSREQLAELQQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLAN 120
           GKICSSELGSIMRSLGQPATEEE+QSMIEEVDADGDGYIDLHEFIELN KDVDSDEVLAN
Sbjct: 61  GKICSSELGSIMRSLGQPATEEELQSMIEEVDADGDGYIDLHEFIELNIKDVDSDEVLAN 120

Query: 121 LKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM 180
           LKDAFSVYDIDGNGSITAEEL EVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM 180

Query: 181 MMVSGSRSGFN 190
           MM+SGSRSGF+
Sbjct: 181 MMMSGSRSGFS 191

[Close]

BLAST of CmoCh15G010260 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.5e-82
Identity = 162/186 (87.10%), Postives = 175/186 (94.09%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFS K KKSD+S+DSPL ST VP+LGSRTPSFNSRAQ+A+L+QVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSRK-KKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           ICSSELGSIM SLGQPATEEE+Q+MI+EVDADGDGYIDLHEFIELNTK VDSDEVL NLK
Sbjct: 61  ICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYD+DGNGSITAEEL EVLKSLGD+CSLADCR MI+GVD+NGDGMISFDEFK MMM
Sbjct: 121 DAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMM 180

Query: 181 VSGSRS 187
            SGSRS
Sbjct: 181 -SGSRS 184

[Close]

BLAST of CmoCh15G010260 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.5e-82
Identity = 162/186 (87.10%), Postives = 175/186 (94.09%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFS K KKSD+S+DSPL ST VP+LGSRTPSFNSRAQ+A+L+QVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSRK-KKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           ICSSELGSIM SLGQPATEEE+Q+MI+EVDADGDGYIDLHEFIELNTK VDSDEVL NLK
Sbjct: 61  ICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYD+DGNGSITAEEL EVLKSLGD+CSLADCR MI+GVD+NGDGMISFDEFK MMM
Sbjct: 121 DAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMM 180

Query: 181 VSGSRS 187
            SGSRS
Sbjct: 181 -SGSRS 184

[Close]

BLAST of CmoCh15G010260 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 7.2e-80
Identity = 163/186 (87.63%), Postives = 172/186 (92.47%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFS K KKSDAS+DSP  ST VPVLGSRTPSFN R Q+A+L+QVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSRK-KKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           I SSELGSIM SLGQPATEEEIQ+MI+EVDADGDG+IDL EFIELNTK VDS+EVL NLK
Sbjct: 61  ISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYDIDGNGSITAEEL EVLKSLGDECSLADCRKMITGVD+NGDGMISFDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMM 180

Query: 181 VSGSRS 187
            SGSRS
Sbjct: 181 -SGSRS 184

[Close]

BLAST of CmoCh15G010260 vs. NCBI nr
Match: XP_022939351.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 6.4e-99
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180

Query: 181 VSGSRSGFNA 191
           VSGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

[Close]

BLAST of CmoCh15G010260 vs. NCBI nr
Match: KAG7029021.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.0 bits (933), Expect = 7.9e-97
Identity = 187/190 (98.42%), Postives = 188/190 (98.95%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPLPST VPVLGSRTPSFNSRAQLA+LQQVFKKFDVNGD K
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTAVPVLGSRTPSFNSRAQLAELQQVFKKFDVNGDAK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180

Query: 181 VSGSRSGFNA 191
           VSGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

[Close]

BLAST of CmoCh15G010260 vs. NCBI nr
Match: KAG6579273.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 360.9 bits (925), Expect = 6.7e-96
Identity = 185/190 (97.37%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPLPST VPVLGSRTPSFNSRAQLA+LQQVFKKFDVNGD K
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLPSTAVPVLGSRTPSFNSRAQLAELQQVFKKFDVNGDAK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK
Sbjct: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYDIDGNGSITAEELQEVLKS+GDECSLADCRKMITGVDKNGDGMISFDEFKVMM 
Sbjct: 121 DAFSVYDIDGNGSITAEELQEVLKSMGDECSLADCRKMITGVDKNGDGMISFDEFKVMMT 180

Query: 181 VSGSRSGFNA 191
           VSGSRSGFNA
Sbjct: 181 VSGSRSGFNA 190

[Close]

BLAST of CmoCh15G010260 vs. NCBI nr
Match: XP_023551072.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349.4 bits (895), Expect = 2.0e-92
Identity = 179/190 (94.21%), Postives = 185/190 (97.37%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGK 60
           MGFKSLFSLKIKKSDASTDSPL ST VP+LGSRTPSFNS AQLA+LQQVFKKFDVNGDGK
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPLLGSRTPSFNSPAQLAELQQVFKKFDVNGDGK 60

Query: 61  ICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLANLK 120
           ICSSELGSIMRSLGQ ATEEE+QSMIEEVDADGDGYIDLHEFIELNTKDVDS+EVLANLK
Sbjct: 61  ICSSELGSIMRSLGQAATEEELQSMIEEVDADGDGYIDLHEFIELNTKDVDSEEVLANLK 120

Query: 121 DAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVMMM 180
           DAFSVYD+DGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMI FDEFKVMMM
Sbjct: 121 DAFSVYDMDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMICFDEFKVMMM 180

Query: 181 VSGSRSGFNA 191
           +SGSRSGFNA
Sbjct: 181 MSGSRSGFNA 190

[Close]

BLAST of CmoCh15G010260 vs. NCBI nr
Match: XP_022973553.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 346.3 bits (887), Expect = 1.7e-91
Identity = 180/191 (94.24%), Postives = 184/191 (96.34%), Query Frame = 0


Query: 1   MGFKSLFSLKIKKSDASTDSPLPSTPVPVLGSRTPSF--NSRAQLADLQQVFKKFDVNGD 60
           MGFKSLFSLKIKKSDASTDSPL ST VPVLGSRTPSF  NSR QLA+LQQVFKKFDVNGD
Sbjct: 1   MGFKSLFSLKIKKSDASTDSPLRSTAVPVLGSRTPSFNSNSREQLAELQQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIELNTKDVDSDEVLAN 120
           GKICSSELGSIMRSLGQPATEEE+QSMIEEVDADGDGYIDLHEFIELN KDVDSDEVLAN
Sbjct: 61  GKICSSELGSIMRSLGQPATEEELQSMIEEVDADGDGYIDLHEFIELNIKDVDSDEVLAN 120

Query: 121 LKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM 180
           LKDAFSVYDIDGNGSITAEEL EVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDKNGDGMISFDEFKVM 180

Query: 181 MMVSGSRSGFN 190
           MM+SGSRSGF+
Sbjct: 181 MMMSGSRSGFS 191

[Close]

BLAST of CmoCh15G010260 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 201.4 bits (511), Expect = 6.3e-52
Identity = 96/148 (64.86%), Postives = 119/148 (80.41%), Query Frame = 0


Query: 38  NSRAQLADLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYI 97
           + + ++ +L+ VFKKFDVNGDGKI S ELG+IM SLG    EEE++  I E+D  GDGYI
Sbjct: 30  SGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYI 89

Query: 98  DLHEFIELNTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRK 157
           +  EF+ELNTK +D ++VL NLKDAFSVYDIDGNGSI+AEEL EVL+SLGDECS+A+CRK
Sbjct: 90  NFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRK 149

Query: 158 MITGVDKNGDGMISFDEFKVMMMVSGSR 186
           MI GVDK+GDG I F+EFK+MM +   R
Sbjct: 150 MIGGVDKDGDGTIDFEEFKIMMTMGSRR 177

[Close]

BLAST of CmoCh15G010260 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 142.1 bits (357), Expect = 4.5e-34
Identity = 68/155 (43.87%), Postives = 108/155 (69.68%), Query Frame = 0


Query: 32  SRTPSFNSRAQLADLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDA 91
           S+  S N    + D+++VF++FD N DGKI   EL  ++ +L   A++EE ++M++E D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 92  DGDGYIDLHEFIEL--NTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDE 151
           DG+G+IDL EF+ L   +    ++  + +LK+AF +YD+D NG I+A EL  V+K+LG++
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 152 CSLADCRKMITGVDKNGDGMISFDEFKVMMMVSGS 185
           CS+ DC++MI  VD +GDG + F+EFK MMM++GS
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMMINGS 156

[Close]

BLAST of CmoCh15G010260 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 137.9 bits (346), Expect = 8.5e-33
Identity = 67/143 (46.85%), Postives = 104/143 (72.73%), Query Frame = 0


Query: 45  DLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIEEVDADGDGYIDLHEFIE 104
           D+++VF++FD NGDGKI   EL  ++R+L   A+ EE  +M+++ D DG+G+IDL EF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 105 LNTKDV----DSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLGDECSLADCRKMIT 164
           L    +    ++   +++LK+AF +YD+DGNG I+A+EL  V+K+LG++CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 165 GVDKNGDGMISFDEFKVMMMVSG 184
            VD +GDG ++FDEFK MM   G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNGG 159

[Close]

BLAST of CmoCh15G010260 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.0e-30
Identity = 70/155 (45.16%), Postives = 95/155 (61.29%), Query Frame = 0


Query: 28  PVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIE 87
           P     TP+    A   +L++VF +FD NGDGKI   ELG + +++G   TE E+  ++E
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 88  EVDADGDGYIDLHEFIELNTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLG 147
           EVD D DGYI+L EF  L      S    A ++DAF +YD D NG I+A EL +VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTL----CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 148 DECSLADCRKMITGVDKNGDGMISFDEFKVMMMVS 183
             CS+ DC +MI  VD +GDG ++F+EF+ MM  S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CmoCh15G010260 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.0e-30
Identity = 70/155 (45.16%), Postives = 95/155 (61.29%), Query Frame = 0


Query: 28  PVLGSRTPSFNSRAQLADLQQVFKKFDVNGDGKICSSELGSIMRSLGQPATEEEIQSMIE 87
           P     TP+    A   +L++VF +FD NGDGKI   ELG + +++G   TE E+  ++E
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 88  EVDADGDGYIDLHEFIELNTKDVDSDEVLANLKDAFSVYDIDGNGSITAEELQEVLKSLG 147
           EVD D DGYI+L EF  L      S    A ++DAF +YD D NG I+A EL +VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTL----CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 148 DECSLADCRKMITGVDKNGDGMISFDEFKVMMMVS 183
             CS+ DC +MI  VD +GDG ++F+EF+ MM  S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

The following BLAST results are available for this feature:

BLAST of CmoCh15G010260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	8.9e-51	64.86	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	2.1e-36	47.70	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O64943	5.8e-34	52.59	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
A0T2M3	3.7e-33	50.37	Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1	[more]
Q9C8Y1	6.4e-33	43.87	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of CmoCh15G010260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FFM7	3.1e-99	100.00	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1IDH5	8.2e-92	94.24	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114720...	[more]
A0A6J1JTS9	4.5e-82	87.10	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874...	[more]
A0A6J1HID9	4.5e-82	87.10	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1CZ20	7.2e-80	87.63	probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of CmoCh15G010260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939351.1	6.4e-99	100.00	probable calcium-binding protein CML25 [Cucurbita moschata]	[more]
KAG7029021.1	7.9e-97	98.42	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. a...	[more]
KAG6579273.1	6.7e-96	97.37	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023551072.1	2.0e-92	94.21	probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo]	[more]
XP_022973553.1	1.7e-91	94.24	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]

BLAST of CmoCh15G010260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	6.3e-52	64.86	EF hand calcium-binding protein family	[more]
AT1G66400.1	4.5e-34	43.87	calmodulin like 23	[more]
AT5G37770.1	8.5e-33	46.85	EF hand calcium-binding protein family	[more]
AT1G18210.1	3.0e-30	45.16	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	3.0e-30	45.16	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 81..109 e-value: 7.4E-5 score: 32.1 coord: 154..182 e-value: 6.6E-5 score: 32.3 coord: 118..146 e-value: 9.5E-6 score: 35.1 coord: 45..73 e-value: 3.5E-6 score: 36.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 45..104 e-value: 8.3E-15 score: 55.0 coord: 118..179 e-value: 1.4E-12 score: 47.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 114..149 score: 15.294476
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 41..76 score: 15.322372
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..185 score: 13.453381
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 77..112 score: 12.839682
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 45..105 e-value: 3.32797E-20 score: 77.9733
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 119..180 e-value: 7.44605E-20 score: 77.2029
None	No IPR available	GENE3D	1.10.238.10		coord: 32..107 e-value: 2.7E-25 score: 90.5 coord: 108..185 e-value: 1.2E-24 score: 88.4
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 29..185
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 29..185
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 90..102
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 127..139
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 54..66
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 33..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh15G010260.1	CmoCh15G010260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding