CmoCh15G010150 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh15G010150
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cmo_Chr15: 5922374 .. 5923000 (-)
RNA-Seq Expression	CmoCh15G010150
Synteny	CmoCh15G010150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCCTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGTGTCATTACTCTCTATCCTGACGTCTGCTACACTTCTCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCCGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTTTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

mRNA sequence

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCCTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGTGTCATTACTCTCTATCCTGACGTCTGCTACACTTCTCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCCGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTTTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Coding sequence (CDS)

ATGAAACCCCAACCGTCTCCTATCGTCTCTCTCCTGTTCGCAGCCGGTCTCTTCCTCCTCCGCCTTGTTCCATCCGCTGCCGATGCCGGAACCTTTCCCAATGCCACCGCCGATTGTTCTGAGTTCATCCGCACTAGCTGTGTCATTACTCTCTATCCTGACGTCTGCTACACTTCTCTCTCTCGCTACGCCAATGCCATTCAGCAGGACCCGGCCTCGCTCACTCGCGTCGCCATTACCATCAGCCTTGCCAACGCCCGTAGAATGGCTGCTTATGTCTCCAACCTGTCTCACGTCGGTGACTACGGCGCCGACCACAGAGTCTCCTCCGCGCTTCACGACTGCTTCACGAACTTCGACGATGCCGTGGACGAGATTCGCGGCTCACTCAAGCAGATGCGTCAGATCAACGATGTCGACGCGCCGTCGTTTAGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCGGCGTTAACGGATCAAGAAACGTGTACGGACGGATTCGATGATGTGGTGGACGGACCGATTAAGAAGGATGTTTGCGCAAAGGCGGAGAAGGTGAAGAAACACACCAGTAATGCCTTAGCGTTGGTGAACAGCTTCGTGGAGAAAAGGATTCCGTGA

Protein sequence

MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRIP

Homology

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BLAST of CmoCh15G010150 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 9.4e-30
Identity = 79/197 (40.10%), Postives = 115/197 (58.38%), Query Frame = 0


Query: 10  SLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQ 69
           +L+F+A L +L    +AA A       A  ++FI+TSC +T YP VC  SLS YA  IQ 
Sbjct: 7   TLVFSALLVIL----AAASA-------APANQFIKTSCTLTTYPAVCEQSLSAYAKTIQN 66

Query: 70  DPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGS 129
           +P  L   A+ +SL   ++   ++  L+     G   R  +A+HDC    +D++D +  S
Sbjct: 67  NPQELASTALQVSLTRTQQAQTFMKRLNKF--KGLKARQYAAIHDCLEEVEDSLDRVSRS 126

Query: 130 LKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKV 189
             +M+ ++      F F+MSNV+TW+SAALTD+ TC DGF    +DG IK+ V A+   V
Sbjct: 127 CDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAV 186

Query: 190 KKHTSNALALVNSFVEK 206
            + TSNALALVN+F  K
Sbjct: 187 ARVTSNALALVNNFAAK 190

[Close]

BLAST of CmoCh15G010150 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.7e-26
Identity = 75/193 (38.86%), Postives = 107/193 (55.44%), Query Frame = 0


Query: 17  LFLLRLVPSA-ADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLT 76
           LF+L L  +A   A      ++  +EFI+ SC  T YPD C+ SLS YA+ I++ P  L 
Sbjct: 11  LFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLA 70

Query: 77  RVAITISLANARRMAAYVSNLSHVGDY-GADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
             A+ +S+A A+    YVS ++   DY G   R   A+ DC     D VD +  SLK+++
Sbjct: 71  ETALAVSIARAKSAKTYVSEMT---DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELK 130

Query: 137 QINDVDA-PSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKVKKHT 196
            + + D+   F F +SNV+TW SAALTD+  C DGF    + G +K  +      V + T
Sbjct: 131 HLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEET 190

Query: 197 SNALALVNSFVEK 206
           SNALAL+N F  K
Sbjct: 191 SNALALINDFASK 200

[Close]

BLAST of CmoCh15G010150 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.7e-26
Identity = 71/184 (38.59%), Postives = 112/184 (60.87%), Query Frame = 0


Query: 19  LLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVA 78
           L  L PS++ + T+ N T    ++I+TSC ITLY  +CY SLS YA+ I+ +P  L  +A
Sbjct: 127 LSSLSPSSS-SSTYSNQTN--LDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIA 186

Query: 79  ITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQIND 138
           + ++L++A+  + +V N+SH G  G       A+ DC     D+V  ++ S++++  IN 
Sbjct: 187 LNLTLSSAKSASKFVKNISHGG--GLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINY 246

Query: 139 VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALAL 198
            D+  F   MS+V+TW+SAALT+ +TC DGF  +V   +K  V     +V + TSNALAL
Sbjct: 247 KDSAKFEMVMSDVETWVSAALTNDDTCMDGF-SLVKTAVKDLVRRHVVEVARLTSNALAL 304

Query: 199 VNSF 203
           +N +
Sbjct: 307 INMY 304

[Close]

BLAST of CmoCh15G010150 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 70/199 (35.18%), Postives = 103/199 (51.76%), Query Frame = 0


Query: 11  LLFAAGLFLL--RLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQ 70
           + +   LFLL   ++ +++ A      +     FI+ SC  T YP VC  SL+ YAN+IQ
Sbjct: 5   IFYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQ 64

Query: 71  QDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRG 130
             P  L   A+ +++  A+    +V  L          R   A+ DC     DAVD +  
Sbjct: 65  TSPRRLAETALNVTVTQAQSTKVFVWRLGRF--TSLKKREIQAVKDCIEEIHDAVDRLTM 124

Query: 131 SLKQMRQINDVDA-PSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAE 190
           S+ +++          F F MSN QTW SAALT+  TC+DGF   V+DG +K  V A+  
Sbjct: 125 SIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARIL 184

Query: 191 KVKKHTSNALALVNSFVEK 206
            + + TSNALAL+N+F +K
Sbjct: 185 NLGRGTSNALALINAFAKK 201

[Close]

BLAST of CmoCh15G010150 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 72/193 (37.31%), Postives = 109/193 (56.48%), Query Frame = 0


Query: 17  LFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQ-QDPASLT 76
           L +L  +     +   PN  ++  +FI +SC  T YP +C  +LS YA  I+  +   L 
Sbjct: 9   LVILLSITILQSSSATPN-RSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLA 68

Query: 77  RVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQM-R 136
           + A+ ISLA A+ +  +V+ L+         R   A+ DC     ++VD +  S+K++ R
Sbjct: 69  QTALIISLARAKSVTIFVAKLTKETP-KFKRREYLAIKDCIEVLGNSVDRLAQSVKELAR 128

Query: 137 QINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDD-VVDGPIKKDVCAKAEKVKKHTS 196
             + V +  F ++MSNVQTW+SAALTD+ TC DGF +  + G +K+ +  K   V + TS
Sbjct: 129 AGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTS 188

Query: 197 NALALVNSFVEKR 207
           NALALVN F EKR
Sbjct: 189 NALALVNQFAEKR 199

[Close]

BLAST of CmoCh15G010150 vs. ExPASy TrEMBL
Match: A0A6J1FD40 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444672 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.3e-111
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL
Sbjct: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmoCh15G010150 vs. ExPASy TrEMBL
Match: A0A6J1I7T1 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111472077 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.1e-109
Identity = 205/208 (98.56%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHV DYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDV+DGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmoCh15G010150 vs. ExPASy TrEMBL
Match: A0A6J1JSR9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111487516 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 8.1e-93
Identity = 181/212 (85.38%), Postives = 192/212 (90.57%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVP----SAADAGTFPNATADCSEFIRTSCVITLYPDVC 60
           MKPQ   I+SLLFAA L LLR VP    +AADAGT  NATA+ +EFIR+SCVITLYPDVC
Sbjct: 1   MKPQSPLILSLLFAATLCLLRQVPAAAAAAADAGTSVNATANGTEFIRSSCVITLYPDVC 60

Query: 61  YTSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCF 120
           Y SLSRYAN IQQDPASLTRVAITISLAN+RRMA+YVSNLSHVGDYGADHR +SALHDCF
Sbjct: 61  YASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCF 120

Query: 121 TNFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGP 180
           TNFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP
Sbjct: 121 TNFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGP 180

Query: 181 IKKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           +K DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 MKADVCAKAEKVKKHTSNALALVNSFVQKRIP 212

[Close]

BLAST of CmoCh15G010150 vs. ExPASy TrEMBL
Match: A0A6J1HFF2 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463076 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.1e-92
Identity = 180/211 (85.31%), Postives = 191/211 (90.52%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADA---GTFPNATADCSEFIRTSCVITLYPDVCY 60
           MKPQ   I+SLLF A L LLR VP+AADA   GT  NATA+ +EFIR+SCVITLYPDVCY
Sbjct: 1   MKPQSPLILSLLFVATLCLLRQVPAAADAADSGTSVNATANSTEFIRSSCVITLYPDVCY 60

Query: 61  TSLSRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFT 120
            SLSRYAN IQQDPASLTRVAITISLAN+RRMA+YVSNLSHVGDYGADHR +SALHDCFT
Sbjct: 61  ASLSRYANDIQQDPASLTRVAITISLANSRRMASYVSNLSHVGDYGADHRAASALHDCFT 120

Query: 121 NFDDAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPI 180
           NFDDAVDEIRGSL QMRQINDVD PSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+
Sbjct: 121 NFDDAVDEIRGSLNQMRQINDVDVPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPM 180

Query: 181 KKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           K DVCAKAEKVKKHTSNALALVNSFV+KRIP
Sbjct: 181 KADVCAKAEKVKKHTSNALALVNSFVQKRIP 211

[Close]

BLAST of CmoCh15G010150 vs. ExPASy TrEMBL
Match: A0A0A0KPD9 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.8e-92
Identity = 178/208 (85.58%), Postives = 188/208 (90.38%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQ   I+SLLFAA LF LR V +  D    PNATA+C EFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQRPLILSLLFAATLFYLRPVSADEDTPNSPNATANCMEFIRTSCGITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYAN IQQDPASLTR+AITISLAN+RRMAAYVSNLSHVGD GAD R +SALHDCFTNFD
Sbjct: 61  SRYANDIQQDPASLTRIAITISLANSRRMAAYVSNLSHVGDNGADRRAASALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGF+DV DGP+K+D
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFEDVADGPMKED 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEK+IP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKKIP 208

[Close]

BLAST of CmoCh15G010150 vs. NCBI nr
Match: KAG6579262.1 (hypothetical protein SDJN03_23710, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 411.4 bits (1056), Expect = 4.7e-111
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL
Sbjct: 33  MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 92

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 93  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 152

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 153 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 212

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 213 VCAKAEKVKKHTSNALALVNSFVEKRIP 240

[Close]

BLAST of CmoCh15G010150 vs. NCBI nr
Match: XP_022938421.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 411.4 bits (1056), Expect = 4.7e-111
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL
Sbjct: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmoCh15G010150 vs. NCBI nr
Match: KAG7016778.1 (hypothetical protein SDJN02_21888, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 408.3 bits (1048), Expect = 4.0e-110
Identity = 206/208 (99.04%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQPSPIVSLLFAAGLF+LRLVPSAADAGTFPNATADCSEFIRTSC ITLYPDVCYTSL
Sbjct: 1   MKPQPSPIVSLLFAAGLFVLRLVPSAADAGTFPNATADCSEFIRTSCFITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmoCh15G010150 vs. NCBI nr
Match: XP_023549992.1 (21 kDa protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 408.3 bits (1048), Expect = 4.0e-110
Identity = 207/208 (99.52%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmoCh15G010150 vs. NCBI nr
Match: XP_022973542.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 404.8 bits (1039), Expect = 4.4e-109
Identity = 205/208 (98.56%), Postives = 206/208 (99.04%), Query Frame = 0


Query: 1   MKPQPSPIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60
           MKPQPS IVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL
Sbjct: 1   MKPQPSSIVSLLFAAGLFLLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSL 60

Query: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFD 120
           SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHV DYGADHRVSSALHDCFTNFD
Sbjct: 61  SRYANAIQQDPASLTRVAITISLANARRMAAYVSNLSHVADYGADHRVSSALHDCFTNFD 120

Query: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKD 180
           DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDV+DGPIKKD
Sbjct: 121 DAVDEIRGSLKQMRQINDVDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVMDGPIKKD 180

Query: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 209
           VCAKAEKVKKHTSNALALVNSFVEKRIP
Sbjct: 181 VCAKAEKVKKHTSNALALVNSFVEKRIP 208

[Close]

BLAST of CmoCh15G010150 vs. TAIR 10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 3.9e-47
Identity = 94/180 (52.22%), Postives = 134/180 (74.44%), Query Frame = 0


Query: 34  NATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAYV 93
           ++ +D S+FIRTSC  TLYPD+C++SLS +++++  DPA L R AI+++L     +A+Y+
Sbjct: 25  HSQSDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLASYL 84

Query: 94  SNLSHV----GDYGADHRVSSAL-HDCFTNFDDAVDEIRGSLKQMRQ-INDVDAPSFRFQ 153
           +N++ +     + GA H  ++A+ HDCF N  DAV+E++GS+KQMR+ ++     SFRFQ
Sbjct: 85  ANITTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFRFQ 144

Query: 154 MSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           MSNVQTW+SAALTD+ETCTDGF D+ D P K D+CA+ + VKK TSNALALVN  V+K I
Sbjct: 145 MSNVQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALVNRCVDKAI 204

[Close]

BLAST of CmoCh15G010150 vs. TAIR 10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 3.7e-45
Identity = 100/191 (52.36%), Postives = 132/191 (69.11%), Query Frame = 0


Query: 17  LFLLRLVPSAADAGTFP--NATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASL 76
           LF+ R +     A  FP    T D  +FIRTSC  TLYPDVCYTSL+ YA+A+Q +PA L
Sbjct: 35  LFICRTI----SAVRFPPEQPTTDDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARL 94

Query: 77  TRVAITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMR 136
            ++AI +SL+ A+  AAY+S LS           S+A+HDC +N  DAVD++RGSL+Q+R
Sbjct: 95  AKLAIGVSLSRAKYTAAYLSKLSR-------RAASAAVHDCVSNVGDAVDQMRGSLRQLR 154

Query: 137 QIND--VDAPSFRFQMSNVQTWMSAALTDQETCTDGF-DDVVDGPIKKDVCAKAEKVKKH 196
           ++N      P+FRFQMSNVQTWMSAALTD+ETCTDG  +++ DG  K  +C +   VK+ 
Sbjct: 155 EMNHRRPGDPAFRFQMSNVQTWMSAALTDEETCTDGVTEEMEDGETKTAICDRVADVKRF 214

Query: 197 TSNALALVNSF 203
           TSNALALVN++
Sbjct: 215 TSNALALVNTY 214

[Close]

BLAST of CmoCh15G010150 vs. TAIR 10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 177.9 bits (450), Expect = 8.2e-45
Identity = 105/211 (49.76%), Postives = 140/211 (66.35%), Query Frame = 0


Query: 11  LLFAAGLFLLRLVPSAADAGTFPN-ATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQ 70
           LL    +  +RL+P        PN  T +  +FIRTSC  TLYPDVC+TSLS YA+A+Q 
Sbjct: 19  LLIFGSISAVRLLPR-------PNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQD 78

Query: 71  DPASLTRVAITISLANARRMAAYVSNLSH-VGDYGAD-HRVSSA-LHDCFTNFDDAVDEI 130
            PA L ++AI +SL+ A+  AA++S LS     Y  D H+ +SA + DC +N +DAVDE+
Sbjct: 79  SPARLAKLAIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEM 138

Query: 131 RGSLKQMRQIND--------VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGP-I 190
           RGSL+Q+R +N             +FRFQMSNVQTWMSAALTD++TCTDGF+D+ +G  I
Sbjct: 139 RGSLRQLRDMNGRGGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLI 198

Query: 191 KKDVCAKAEKVKKHTSNALALVNSFVEKRIP 209
           K  VC + E+VK+ TSNALALVN++     P
Sbjct: 199 KTTVCDRLEEVKRLTSNALALVNTYANNGAP 222

[Close]

BLAST of CmoCh15G010150 vs. TAIR 10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 164.9 bits (416), Expect = 7.1e-41
Identity = 90/178 (50.56%), Postives = 121/178 (67.98%), Query Frame = 0


Query: 33  PNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVAITISLANARRMAAY 92
           P   ++ S+FIR +C  TLYPD+C+++LS +AN+IQ D   L RVAI+++L N   + +Y
Sbjct: 27  PLLPSNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLHNTLHLLSY 86

Query: 93  VSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQINDVDA--PSFRFQMSN 152
           + N      Y  DH  +  L DCF N  DAVD +RGS+KQM+++        SFRFQMSN
Sbjct: 87  LQNA-----YNRDH-PTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESFRFQMSN 146

Query: 153 VQTWMSAALTDQETCTDGFDDV-VDGPIKKDVCAKAEKVKKHTSNALALVNSFVEKRI 208
           V+TW+SAALTD+ TCTDGF DV  D  IK DVC++ + VKK TSNALALVN + ++ I
Sbjct: 147 VKTWLSAALTDEYTCTDGFKDVHEDDSIKDDVCSRVDDVKKLTSNALALVNRYADESI 198

[Close]

BLAST of CmoCh15G010150 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.2e-27
Identity = 71/184 (38.59%), Postives = 112/184 (60.87%), Query Frame = 0


Query: 19  LLRLVPSAADAGTFPNATADCSEFIRTSCVITLYPDVCYTSLSRYANAIQQDPASLTRVA 78
           L  L PS++ + T+ N T    ++I+TSC ITLY  +CY SLS YA+ I+ +P  L  +A
Sbjct: 127 LSSLSPSSS-SSTYSNQTN--LDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIA 186

Query: 79  ITISLANARRMAAYVSNLSHVGDYGADHRVSSALHDCFTNFDDAVDEIRGSLKQMRQIND 138
           + ++L++A+  + +V N+SH G  G       A+ DC     D+V  ++ S++++  IN 
Sbjct: 187 LNLTLSSAKSASKFVKNISHGG--GLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINY 246

Query: 139 VDAPSFRFQMSNVQTWMSAALTDQETCTDGFDDVVDGPIKKDVCAKAEKVKKHTSNALAL 198
            D+  F   MS+V+TW+SAALT+ +TC DGF  +V   +K  V     +V + TSNALAL
Sbjct: 247 KDSAKFEMVMSDVETWVSAALTNDDTCMDGF-SLVKTAVKDLVRRHVVEVARLTSNALAL 304

Query: 199 VNSF 203
           +N +
Sbjct: 307 INMY 304

The following BLAST results are available for this feature:

BLAST of CmoCh15G010150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	9.4e-30	40.10	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	1.7e-26	38.86	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	1.7e-26	38.59	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9STY5	2.9e-23	35.18	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI72	2.5e-22	37.31	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]

BLAST of CmoCh15G010150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FD40	2.3e-111	100.00	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444672 PE=4 SV=1	[more]
A0A6J1I7T1	2.1e-109	98.56	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111472077 PE=4 SV=1	[more]
A0A6J1JSR9	8.1e-93	85.38	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111487516 PE=4 SV=1	[more]
A0A6J1HFF2	1.1e-92	85.31	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463076 PE=4 SV=1	[more]
A0A0A0KPD9	1.8e-92	85.58	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G567800 PE=4 S...	[more]

BLAST of CmoCh15G010150 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6579262.1	4.7e-111	100.00	hypothetical protein SDJN03_23710, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022938421.1	4.7e-111	100.00	21 kDa protein-like [Cucurbita moschata]	[more]
KAG7016778.1	4.0e-110	99.04	hypothetical protein SDJN02_21888, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023549992.1	4.0e-110	99.52	21 kDa protein [Cucurbita pepo subsp. pepo]	[more]
XP_022973542.1	4.4e-109	98.56	21 kDa protein-like [Cucurbita maxima]	[more]

BLAST of CmoCh15G010150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23205.1	3.9e-47	52.22	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G14890.1	3.7e-45	52.36	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G01610.1	8.2e-45	49.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70720.1	7.1e-41	50.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	1.2e-27	38.59	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 38..198 e-value: 1.3E-43 score: 160.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 15..200 e-value: 7.7E-31 score: 104.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 42..198 e-value: 2.1E-39 score: 135.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 38..202 e-value: 8.2E-40 score: 138.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 40..202
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 16..205
None	No IPR available	PANTHER	PTHR31080:SF64	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 16..205
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 45..202 e-value: 1.31959E-47 score: 151.822

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh15G010150.1	CmoCh15G010150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity