CmoCh15G007330 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G007330
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmitogen-activated protein kinase kinase kinase 1-like
LocationCmo_Chr15: 3583896 .. 3588366 (-)
RNA-Seq ExpressionCmoCh15G007330
SyntenyCmoCh15G007330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCTACTCTCTCTTCCACGTAGCACCGCGTTTTTTTCTCTCTCCCGGCGTTTACCAGTCCAATTCAGTCCATCGTTCAACCCTTTTCTATCTCTCTCTCTCTGCGCCCTTTTTTCCCAGCTTCCCTTCCTCAAACTTGGAGGCTAATCCCATTGCTAAAATCGAGGTAGATTTTCCCTTCTGAACTTGTTATTTTCGTTGTGGGGGTGATTTGATCGGGGAATTCTTCTTGCCGTTGCCGGTGGGATTGTTTAATGGGCTTAAGTCTCGATGCATCACCTGCCTCAGTTTTTCTCTCCCAGGAAGCGAAGCAAAGCCATGGATCCGAGGAAAATTGCAGGGAAACCCAAGCTTTACCGACTTAATGCGGCCAAGAACATCGACTATGACGCTGCTTACACGTCTTCTTCGTTCGATGATTCCTCTGGGTCTCTCTACACTCGCTCCATGGAGACCTTCAATCCCTCGAGCTTCCGGATTAAAGGGCTTGACGGTGAAGTTGATCTCATATGTCGGACTTTAGGGCTAGATGGCCCTGACGACTTCGCTATTCCGATGGAGGCTTGGGAGGCCAGGAAGGTTCGTTCATCTTCTGATATTCTACCGAGGTCGAGGCTGTATCCCGTGGATAGTCCAAAGACAGAGGAGATTAGTAAGGAGAAGGAAGATGAAGAAAACGAAGACGAATTGTGCCGGAGGGTTAAGGATTCAGTACGAATTAGCGATGAGATGTCCACGCTGAAGACTGAACTTCCCGAGTTGAATGAACGCTGTATGGATACTGTTACTGGACGTAGCCCGAGAAGTGGAATCAATGGTGCTCGGCCTCCCTTACTCAAGCCGCCGCCGTGTATGCGGCTTCCAGATTTTGATAACGCTTTCTCGACTTGGGACATTTTGAAAGCTTTTGCTCCATTAGAGGATGATAAAAAACAGGATGAAGTAGAAGAACGAGTCGAACCTTTGGTTGAACGAGAAGTCGAGGAAAATATTGTCAGGCCCGAGGAGAGCGCAGCGCTCATAGAGTCCTGGGGTTCCTTCACGACCTCGAATGACGATGATTCTTCGAGCAGTACGACGGAGCCTGCAAATATTTCACCCAATATGAGAGGTAAACCCATTATCACATCCTGGGTGGAAACCGGTAGGCTTCTTGGACGCGGTTCATTTGGATCAGTTTCCGAAGCAATTTCAGAGTACGTTATGTTTCTCTTAGCTTATTTTCCCCTGCAACTTCTGTTTTCTCTTGCATCATTGAAGATTGCAGAGAATTAACTCGCACGTTTAGTATGACTCTAGCTTTTCTGATCTGGGAATTCGGAAGAACAATTGTTTTTATAACCCAGAATTTCCTGGTGGATGAAATGATACCCTTTTGTTGATAGAAAGCAGATTCATCCTAAACCCCGCCTCGATTTTCGTTTGGTCGGAAAATTCATCTGATTTTGTATCATTTGCAGGGATGGAATCTTTTTTGCTCTTAAAGAAGTTTCACTGCTCAATGAAGATAGTCAGGGAAGACAGAGCATCTTTCAACTTCAGCAGGTATTACACTTGTTCGTGATATATATATATATATATATATATATTGGTTATGATTTGAAATTCTGATCAGTCTTTTCCTTATGACGCCTTGACAATCTCTTATTCTTAACCTGCTTTTCTCACTTATTCATTTGATTTCTCTTCCAGGAGATATCTCTTCTAAGTGAATTTGAGCATGAGAACATAGTCCAGTACTACGGCACACATTCGGTATTGCCCTTCTCCGTACTTAAGAATTGAAGTTCTTAGGCATTTTAACTAATCTTTGGGTGGTTCGAGAAAAGATGTGCTTAATTATTTTAGTTAATTATTGCCCTTCCTTTCTCTTTTCATATTCTTTGGCCATAACATTAAGCTCAGGATCTCCTGTGCGTTTTATATGCTTCAGAAACCCTCATGGAATTCCATATAATTTATCTTCTCGTGCCCGAAGTTTAGTTTCTACAATTCTTTTCAAACTTGTCTAAAGTCAGTTGATTCTATACGATGTTTTTGGTTGACTTAAAAACATACCTTTTACTGTATGGCAACATATGCTTCTCTATGGACCGAGTATGCGCATACAACGTGTAAAGTCCAAATTCAAAAGTTACTGGCCTATCATGAACCAAACGTCAGCATACAAGTTTTACCTTGATGTCTACTTCATCATTTGATGCCTTTTTTTTGTCCTGCCAGAAGGCGGTTTGTTATTCTAACTTGACATGCCAATTGTCCAACCTTGTGCTATTTTAATTTTCCATCTTATTCAAAGTATGTTGGTAGGTGCACTGATGTGCCGAGTTTGGAGCACGAAATGAATTACATGTGCTCTCATGAGCTTTAATATCAGACCATGTCACGGATCCTTACATGGCTATCTAGTTTTCGTTCATCTATTTTAGCTAATGGTGCAATGAATGTACTGTAGTTTACTAATGGTTGTACTCTTTTGGCTACTTGAATATTAAAATTCTCATATATTCAGTACTTACTACTTTATTTGATTCTTGTTTGCAGGATGGGTCAAAACTCTATATCTTCCTGGAGCTTGTATCTCAAGGCTCCCTTCTGAATTTATATCAGAGAACAAACCTTCTGGATTCCATTGTTTCTGCCTATACAAGGCAGATTCTGTCTGGTTTAAAGTATCTTCATGAGCGAAATGTGGTTCACAGGTATACAAATGAATACTGGTCATTTATCTTTATAAGTTTGTGTACCGAGCGAAATGTTGGCTCGGTACACATATCATTAGATCTTTATAAGTCCGTAGCCTGAGACTTCTATGGGTCGGTCTGTATTCAACGTGTTCATGTTTAATACCGTTGCTGTGGATGGGAAAGAAGGGGAAAGAAGCTTCTCAGTTGTCAAGTCCATGCTACATTTTTTTTTTTGGTTTCGTAGTGAAAAAAAAAAAAAAAAAAGATCTCACTATTTCGTCAATCCTTCAACAATTTAAATTCCTTCTGGAATATGTTTAATGCCCCAGAATTCTTAATATCCCTGTCTTGCTACTAGTTTTAAAAATATTTTTGGAAATTTATAGTCTGTAAAACAGCATGACATAACCGTCCTTTATCGATTTCTTTGTCCAATAATGTTCTTATTTGTTTTTTTGTTTTTTTTTTTTTTTTTTAAAATTCTGCTCGATCATTTTCTTTAGGAAATTATATCTAGCATTCCTTGTTTCTGTTAAAAGTATACATCTAAAAAAGGTCTGGTCAACAATTTCAAATGAGTTTTTTTTCTTTTCCTTTTTCTCTCTTTGAGTATATATATATATTTTATTGAAGGAAAACATCTTATTTATTGCTCTAACTCTTACTTTTCTCCCCTTCCCCTTCCCCCTCCCGACATATAATCATTTTCTTGTTTTGATTTTTTTTTGAAAAGGGATATTAAATGTGCTAATATATTGGTGGATGTGAATGGATCTGTGAAGCTTGCAGATTTTGGTTTGGCGAAGGTTTCTCCCCAATCCATTTTATTATTTCTGTTTGTGGCCTTGTTTTTGTGAGTTTCTTCCCTTAAAATCATTGTTTGTCTACTTTTCAGGCAACAAAATTCAACGACGTTAAATCTTGCAAGGGAACTGCGTTCTGGATGGCACCTGAGGTTTGCCTCTTCCTTGTCTATGTCAAGAATGTTTGTATTTGACTATGAATATCTAACCAAAGAAGTAGCTTTTTGAGAGGGGTGGCTCTCCCACAATCAACCAACTTTGGTTGACTTAATATGACCTCCCCTCACAAACCTTCTCCCTCTATTTATAACCAAGACCCCTGCTAGTATCCACCTAATGTTTTCAATATATCTTCTATTAGTACTCTCACACATTTCTCACTGTCATTACTGTGATTTGTGCAGGTGGTTAATGGGAAGGGTCAAGGATACGGGCTTCCGGCCGATATTTGGAGCCTTGGGTGTACAGTATTGGAAATGTTAACTCGAAAGCTTCCGTACTCAGAAGTCGAAAGTGTACGTACCACTTTATCCTCTACAAACTTTGTTTTTGTTGTTTGTTGTTTGTTGGATTTCTAAGTTCACAGCTTGAACTGTAACTGAGATAATACAAGATTAATGGTTTGAAGGTGTTTAGAAGATTGCCGCTAAGCTCTCTATTTGTACATCAGTGATTTTCAAACTATTTTAGTTTGTTGTGATAGGAGACATCATAAATAACCATTACTTTTAAGGTTCTAAAACTGCCATCCTTTGAATTCAGCCTATGCAGGCACTATTTAGGATTGGAAAAGGCAAAGCGCCTGTAGTTCCCGAGTCTCTCCCAAAAGATGCACAGGATTTCATTTTGCAGTGCCTACAAGTTAATCCACACGACCGTCCTATCGCTGCTGATCTAATAAATCATCCGTTTGTGAAACGACCAGTTTCCAGTTTATCAGTGTCGGCATCTCCTTACAACAAGCCAGGCAGAAGAATTTAA

mRNA sequence

CTGCTACTCTCTCTTCCACGTAGCACCGCGTTTTTTTCTCTCTCCCGGCGTTTACCAGTCCAATTCAGTCCATCGTTCAACCCTTTTCTATCTCTCTCTCTCTGCGCCCTTTTTTCCCAGCTTCCCTTCCTCAAACTTGGAGGCTAATCCCATTGCTAAAATCGAGGTAGATTTTCCCTTCTGAACTTGTTATTTTCGTTGTGGGGGTGATTTGATCGGGGAATTCTTCTTGCCGTTGCCGGTGGGATTGTTTAATGGGCTTAAGTCTCGATGCATCACCTGCCTCAGTTTTTCTCTCCCAGGAAGCGAAGCAAAGCCATGGATCCGAGGAAAATTGCAGGGAAACCCAAGCTTTACCGACTTAATGCGGCCAAGAACATCGACTATGACGCTGCTTACACGTCTTCTTCGTTCGATGATTCCTCTGGGTCTCTCTACACTCGCTCCATGGAGACCTTCAATCCCTCGAGCTTCCGGATTAAAGGGCTTGACGGTGAAGTTGATCTCATATGTCGGACTTTAGGGCTAGATGGCCCTGACGACTTCGCTATTCCGATGGAGGCTTGGGAGGCCAGGAAGGTTCGTTCATCTTCTGATATTCTACCGAGGTCGAGGCTGTATCCCGTGGATAGTCCAAAGACAGAGGAGATTAGTAAGGAGAAGGAAGATGAAGAAAACGAAGACGAATTGTGCCGGAGGGTTAAGGATTCAGTACGAATTAGCGATGAGATGTCCACGCTGAAGACTGAACTTCCCGAGTTGAATGAACGCTGTATGGATACTGTTACTGGACGTAGCCCGAGAAGTGGAATCAATGGTGCTCGGCCTCCCTTACTCAAGCCGCCGCCGTGTATGCGGCTTCCAGATTTTGATAACGCTTTCTCGACTTGGGACATTTTGAAAGCTTTTGCTCCATTAGAGGATGATAAAAAACAGGATGAAGTAGAAGAACGAGTCGAACCTTTGGTTGAACGAGAAGTCGAGGAAAATATTGTCAGGCCCGAGGAGAGCGCAGCGCTCATAGAGTCCTGGGGTTCCTTCACGACCTCGAATGACGATGATTCTTCGAGCAGTACGACGGAGCCTGCAAATATTTCACCCAATATGAGAGGTAAACCCATTATCACATCCTGGGTGGAAACCGGTAGGCTTCTTGGACGCGGTTCATTTGGATCAGTTTCCGAAGCAATTTCAGAGGATGGAATCTTTTTTGCTCTTAAAGAAGTTTCACTGCTCAATGAAGATAGTCAGGGAAGACAGAGCATCTTTCAACTTCAGCAGGTATTACACTTGTTCGAGATATCTCTTCTAAGTGAATTTGAGCATGAGAACATAGTCCAGTACTACGGCACACATTCGGTATTGCCCTTCTCCGATGGGTCAAAACTCTATATCTTCCTGGAGCTTGTATCTCAAGGCTCCCTTCTGAATTTATATCAGAGAACAAACCTTCTGGATTCCATTGTTTCTGCCTATACAAGGCAGATTCTGTCTGGTTTAAAGTATCTTCATGAGCGAAATGTGGTTCACAGGGATATTAAATGTGCTAATATATTGGTGGATGTGAATGGATCTGTGAAGCTTGCAGATTTTGGTTTGGCGAAGGCAACAAAATTCAACGACGTTAAATCTTGCAAGGGAACTGCGTTCTGGATGGCACCTGAGGTGGTTAATGGGAAGGGTCAAGGATACGGGCTTCCGGCCGATATTTGGAGCCTTGGGTGTACAGTATTGGAAATGTTAACTCGAAAGCTTCCGTACTCAGAAGTCGAAAGTCCTATGCAGGCACTATTTAGGATTGGAAAAGGCAAAGCGCCTGTAGTTCCCGAGTCTCTCCCAAAAGATGCACAGGATTTCATTTTGCAGTGCCTACAAGTTAATCCACACGACCGTCCTATCGCTGCTGATCTAATAAATCATCCGTTTGTGAAACGACCAGTTTCCAGTTTATCAGTGTCGGCATCTCCTTACAACAAGCCAGGCAGAAGAATTTAA

Coding sequence (CDS)

ATGCATCACCTGCCTCAGTTTTTCTCTCCCAGGAAGCGAAGCAAAGCCATGGATCCGAGGAAAATTGCAGGGAAACCCAAGCTTTACCGACTTAATGCGGCCAAGAACATCGACTATGACGCTGCTTACACGTCTTCTTCGTTCGATGATTCCTCTGGGTCTCTCTACACTCGCTCCATGGAGACCTTCAATCCCTCGAGCTTCCGGATTAAAGGGCTTGACGGTGAAGTTGATCTCATATGTCGGACTTTAGGGCTAGATGGCCCTGACGACTTCGCTATTCCGATGGAGGCTTGGGAGGCCAGGAAGGTTCGTTCATCTTCTGATATTCTACCGAGGTCGAGGCTGTATCCCGTGGATAGTCCAAAGACAGAGGAGATTAGTAAGGAGAAGGAAGATGAAGAAAACGAAGACGAATTGTGCCGGAGGGTTAAGGATTCAGTACGAATTAGCGATGAGATGTCCACGCTGAAGACTGAACTTCCCGAGTTGAATGAACGCTGTATGGATACTGTTACTGGACGTAGCCCGAGAAGTGGAATCAATGGTGCTCGGCCTCCCTTACTCAAGCCGCCGCCGTGTATGCGGCTTCCAGATTTTGATAACGCTTTCTCGACTTGGGACATTTTGAAAGCTTTTGCTCCATTAGAGGATGATAAAAAACAGGATGAAGTAGAAGAACGAGTCGAACCTTTGGTTGAACGAGAAGTCGAGGAAAATATTGTCAGGCCCGAGGAGAGCGCAGCGCTCATAGAGTCCTGGGGTTCCTTCACGACCTCGAATGACGATGATTCTTCGAGCAGTACGACGGAGCCTGCAAATATTTCACCCAATATGAGAGGTAAACCCATTATCACATCCTGGGTGGAAACCGGTAGGCTTCTTGGACGCGGTTCATTTGGATCAGTTTCCGAAGCAATTTCAGAGGATGGAATCTTTTTTGCTCTTAAAGAAGTTTCACTGCTCAATGAAGATAGTCAGGGAAGACAGAGCATCTTTCAACTTCAGCAGGTATTACACTTGTTCGAGATATCTCTTCTAAGTGAATTTGAGCATGAGAACATAGTCCAGTACTACGGCACACATTCGGTATTGCCCTTCTCCGATGGGTCAAAACTCTATATCTTCCTGGAGCTTGTATCTCAAGGCTCCCTTCTGAATTTATATCAGAGAACAAACCTTCTGGATTCCATTGTTTCTGCCTATACAAGGCAGATTCTGTCTGGTTTAAAGTATCTTCATGAGCGAAATGTGGTTCACAGGGATATTAAATGTGCTAATATATTGGTGGATGTGAATGGATCTGTGAAGCTTGCAGATTTTGGTTTGGCGAAGGCAACAAAATTCAACGACGTTAAATCTTGCAAGGGAACTGCGTTCTGGATGGCACCTGAGGTGGTTAATGGGAAGGGTCAAGGATACGGGCTTCCGGCCGATATTTGGAGCCTTGGGTGTACAGTATTGGAAATGTTAACTCGAAAGCTTCCGTACTCAGAAGTCGAAAGTCCTATGCAGGCACTATTTAGGATTGGAAAAGGCAAAGCGCCTGTAGTTCCCGAGTCTCTCCCAAAAGATGCACAGGATTTCATTTTGCAGTGCCTACAAGTTAATCCACACGACCGTCCTATCGCTGCTGATCTAATAAATCATCCGTTTGTGAAACGACCAGTTTCCAGTTTATCAGTGTCGGCATCTCCTTACAACAAGCCAGGCAGAAGAATTTAA

Protein sequence

MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSMETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVDSPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSGINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEENIVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHDRPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI
Homology
BLAST of CmoCh15G007330 vs. ExPASy Swiss-Prot
Match: Q39008 (Mitogen-activated protein kinase kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MEKK1 PE=1 SV=2)

HSP 1 Score: 475.7 bits (1223), Expect = 7.3e-133
Identity = 295/594 (49.66%), Postives = 377/594 (63.47%), Query Frame = 0

Query: 27  KLYRLNAAKNIDYDAAYTSSSFDD----SSGSLYTRSMETFNPSSFRIKGLDGEVDLICR 86
           +L R +AA+NI+YDAA  SSS  +    S+ SL TRS+E   P+SFRI G  GE+D I R
Sbjct: 26  RLERRDAARNINYDAASCSSSSAEDLSVSTSSLMTRSLEFPEPTSFRIGGGVGEMDRIYR 85

Query: 87  TLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVDSPKTEEISKEKED--EENEDEL 146
           +LG+ GPDD AI  +AWEA K RSSSD++ R + + +D  + +++S+E         D +
Sbjct: 86  SLGVSGPDDLAISFDAWEACKKRSSSDVVNRFKSFDLDKVRDQDLSEEGPSGVVVGSDSM 145

Query: 147 CRRVK-DSVRISDEMSTLKTELPEL-----------------NERCMD------------ 206
             +V+   +  +     + TEL E+                 N R M+            
Sbjct: 146 NHKVQGQDLSEAGPSGGIVTELSEIGNLITPVDRLVADGVVENRRVMERTPTIVKSKGYL 205

Query: 207 -----TVTGRSPRSGINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKK---- 266
                   G     GI G RPP+LKPPP M+ P  D+  S+WD L  FAP E  K+    
Sbjct: 206 VPNNVVAVGVGVGGGIKGLRPPVLKPPPAMKRPPIDHRGSSWDFLTHFAPSETVKRPSSS 265

Query: 267 ----QDEVEERVEPLVEREVEENIVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISP 326
               +D  +E      E E EE   R  +     +   SFTT N+ DSSS+ +  + I P
Sbjct: 266 SSSSEDGCDEEEGKEEEAEAEEMGARFIQLGDTADETCSFTT-NEGDSSSTVSNTSPIYP 325

Query: 327 NMRGKPIITSWVETGRLLGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ 386
           +  G  IITSW + G+LLGRGSFGSV E IS DG FFA+KEVSLL++ SQ ++ I QL+ 
Sbjct: 326 D--GGAIITSW-QKGQLLGRGSFGSVYEGISGDGDFFAVKEVSLLDQGSQAQECIQQLEG 385

Query: 387 VLHLFEISLLSEFEHENIVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDS 446
                EI LLS+ +H+NIV+Y GT       DGS LYIFLELV+QGSLL LYQR  L DS
Sbjct: 386 -----EIKLLSQLQHQNIVRYRGT-----AKDGSNLYIFLELVTQGSLLKLYQRYQLRDS 445

Query: 447 IVSAYTRQILSGLKYLHERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKG 506
           +VS YTRQIL GLKYLH++  +HRDIKCANILVD NG+VKLADFGLAK +KFND+KSCKG
Sbjct: 446 VVSLYTRQILDGLKYLHDKGFIHRDIKCANILVDANGAVKLADFGLAKVSKFNDIKSCKG 505

Query: 507 TAFWMAPEVVNGK-GQGYGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAP 566
           T FWMAPEV+N K   GYG PADIWSLGCTVLEM T ++PYS++E P+QALFRIG+G  P
Sbjct: 506 TPFWMAPEVINRKDSDGYGSPADIWSLGCTVLEMCTGQIPYSDLE-PVQALFRIGRGTLP 565

BLAST of CmoCh15G007330 vs. ExPASy Swiss-Prot
Match: O81472 (Mitogen-activated protein kinase kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MEKK2 PE=1 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.4e-107
Identity = 279/687 (40.61%), Postives = 374/687 (54.44%), Query Frame = 0

Query: 9   SPRKRSKAMDPRKIAGKPKLYRLNAA-KNIDYDAAYTSSSFDDSSGSLYTRSMETFNPSS 68
           SP +R     P +     +L R +A  +NIDYDA       +DSS  L TRS++  N +S
Sbjct: 110 SPGRRGDKTSPAR-----RLDRSDAVRRNIDYDAG------EDSSSLLITRSLDFPNRTS 169

Query: 69  FRIKGL-DGEVDLICRTLGLDGPDDFAIPMEAWEAR--KVRSSSDILPRSRLYPVD---- 128
           FR+ G+ DGE+D I + +G+ GP+DFAI  +AW+AR    RSSSD++ + +   +D    
Sbjct: 170 FRVDGVDDGEIDRIYQYIGVSGPEDFAISSDAWKARMEHERSSSDVVNKLKSLDLDSREA 229

Query: 129 ---------------------------------------SPKTEEISKEKEDE------- 188
                                                  S K E ++  ++ E       
Sbjct: 230 GPSGGVVASSSMNHKFQGHDLSEAGSIGVVVASNFTLSESNKIENLNSLRDKEIVDGDMV 289

Query: 189 ENEDELCRRV------------KDSVRISDEMSTLK---TELPELNERCMD--TVTGRS- 248
           EN   + R+              D V +   +  ++     +P  ++  +D  TV  +S 
Sbjct: 290 ENRCGIERKPTILVKSRGYLVHNDDVGVGGGIKGVRPPVLNVPRADKEVVDGGTVESKSG 349

Query: 249 ----------------------------------------------PRSGINGARPPLLK 308
                                                          R GI G RP +LK
Sbjct: 350 IEWKPTILVKSKGYLVSNDGGIKGVTSPVLNLRPTDKEVVDSGTVENRRGIKGVRPSVLK 409

Query: 309 PPPCMRLPDFDNAFSTWDILKAFAP-LEDDKKQDEVEERVEPLVEREVEENIVRPEESAA 368
           PPP M+LP  D   S+WDIL  FAP  E  ++            E E E++ V  EE+  
Sbjct: 410 PPPVMKLPPVDLPGSSWDILTHFAPDSEIVRRPSSSSSSENGCDEEEAEDDKVEKEETGD 469

Query: 369 LI-------ESWGSFTTSNDDDSSS-STTEPANISPNMRGKPIITSWVETGRLLGRGSFG 428
           +        +   SFTT+  D SS+ S T P  +S    G  I TSW + G+LL +GSFG
Sbjct: 470 MFIQLEDTTDEACSFTTNEGDSSSTVSNTSPICVS----GGSINTSW-QKGQLLRQGSFG 529

Query: 429 SVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYGT 488
           SV EAISEDG FFA+KEVSLL++ SQ ++ I QL+      EI+LLS+ EH+NI++Y GT
Sbjct: 530 SVYEAISEDGDFFAVKEVSLLDQGSQAQECIQQLEG-----EIALLSQLEHQNILRYRGT 589

Query: 489 HSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHR 548
                  DGS LYIFLELV+QGSLL LY+R  + DS++S YT+QIL GLKYLH +  +HR
Sbjct: 590 D-----KDGSNLYIFLELVTQGSLLELYRRYQIRDSLISLYTKQILDGLKYLHHKGFIHR 649

Query: 549 DIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGK-GQGYGLPADI 567
           DIKCA ILVD NG+VKLADFGLAK +K ND+KS K T FWMAPEV+N K   GY  PADI
Sbjct: 650 DIKCATILVDANGTVKLADFGLAKVSKLNDIKSRKETLFWMAPEVINRKDNDGYRSPADI 709

BLAST of CmoCh15G007330 vs. ExPASy Swiss-Prot
Match: Q9M0T3 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MEKK3 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.2e-87
Identity = 170/277 (61.37%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 278 NMRGKPIITSWVETGRLLGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ 337
           N R    ITSW++ G+LLGRGS+ SV EAISEDG FFA+KEVSLL++  Q ++ I QL+ 
Sbjct: 293 NFRKPEDITSWLK-GQLLGRGSYASVYEAISEDGDFFAVKEVSLLDKGIQAQECIQQLEG 352

Query: 338 VLHLFEISLLSEFEHENIVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDS 397
                EI+LLS+ +H+NIV+Y GT       D SKLYIFLELV+QGS+  LY+R  L  +
Sbjct: 353 -----EIALLSQLQHQNIVRYRGT-----AKDVSKLYIFLELVTQGSVQKLYERYQLSYT 412

Query: 398 IVSAYTRQILSGLKYLHERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKG 457
           +VS YTRQIL+GL YLH++  VHRDIKCAN+LVD NG+VKLADFGLA+A+KFND+ SCKG
Sbjct: 413 VVSLYTRQILAGLNYLHDKGFVHRDIKCANMLVDANGTVKLADFGLAEASKFNDIMSCKG 472

Query: 458 TAFWMAPEVVNGK-GQGYGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAP 517
           T FWMAPEV+N K   G G PADIWSLGCTVLEM T ++PYS+++ P+QA F+IG+G  P
Sbjct: 473 TLFWMAPEVINRKDSDGNGSPADIWSLGCTVLEMCTGQIPYSDLK-PIQAAFKIGRGTLP 532

Query: 518 VVPESLPKDAQDFILQCLQVNPHDRPIAADLINHPFV 554
            VP++L  DA+ FIL CL+VNP +RP AA+L++HPFV
Sbjct: 533 DVPDTLSLDARHFILTCLKVNPEERPTAAELLHHPFV 557


HSP 2 Score: 50.4 bits (119), Expect = 7.6e-05
Identity = 43/140 (30.71%), Postives = 66/140 (47.14%), Query Frame = 0

Query: 180 GINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEE 239
           GI   RPP+++PPP  +LP  D   S+WD L  FAP +  K+Q           +   ++
Sbjct: 30  GIKAVRPPIIQPPPGRKLPLIDFPGSSWDFLTYFAPSKTVKRQSSSSS------DNTSDK 89

Query: 240 NIVRPEESAALIESWGSFT------TSNDDDSSSSTTEPANISPNMRGK-PIITSWVETG 299
             V  EE+  +    G          +N+ DSSS+    + ISP+   +  I+ SW++  
Sbjct: 90  EEVETEETRGMFVQLGDTAHEACPFATNEADSSSTV---SIISPSYASRGSIVPSWLKR- 149

Query: 300 RLLGRGSFGSVSEAISEDGI 313
           + LGR S G V E  S   +
Sbjct: 150 KFLGRVSLGFVYEGSSGSSV 159

BLAST of CmoCh15G007330 vs. ExPASy Swiss-Prot
Match: Q9SZ67 (Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.7e-85
Identity = 199/389 (51.16%), Postives = 242/389 (62.21%), Query Frame = 0

Query: 181  INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
            I G RPP+L+  P M+L       STWD +  FAP          E    P    E  E 
Sbjct: 1527 IKGLRPPVLQLQPAMKLSHIPRG-STWDFVTHFAP---------PETVAPPSSSSEAREE 1586

Query: 241  IVRPEESAALIESWG-----SFTTSNDDDSSS-STTEPANISPNMRGKPIITSWVETGRL 300
             V  EE+ A+    G     SFT +  D S + S T P   S        IT W + G+L
Sbjct: 1587 EVETEETGAMFIPLGDKETCSFTVNKGDSSRTISNTSPIYASEG----SFITCW-QKGQL 1646

Query: 301  LGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHEN 360
            LGRGS GSV E IS DG FFA KEVSLL++ SQ  + I Q++       I+LLS+ +H+N
Sbjct: 1647 LGRGSLGSVYEGISADGDFFAFKEVSLLDQGSQAHEWIQQVEG-----GIALLSQLQHQN 1706

Query: 361  IVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLH 420
            IV+Y GT       D S LYIFLELV+QGSL  LYQR  L DS+VS YTRQIL GLKYLH
Sbjct: 1707 IVRYRGT-----TKDESNLYIFLELVTQGSLRKLYQRNQLGDSVVSLYTRQILDGLKYLH 1766

Query: 421  ERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAF--WMAPEVV-NGKG 480
            ++  +HR+IKCAN+LVD NG+VKLADFGLAK      V S   T +  WMAPEV+ N K 
Sbjct: 1767 DKGFIHRNIKCANVLVDANGTVKLADFGLAK------VMSLWRTPYWNWMAPEVILNPKD 1826

Query: 481  -QGYGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFI 540
              GYG PADIWSLGCTVLEMLT ++PYS++E    AL+ IG GK P +P+ L  DA+DFI
Sbjct: 1827 YDGYGTPADIWSLGCTVLEMLTGQIPYSDLEIG-TALYNIGTGKLPKIPDILSLDARDFI 1883

Query: 541  LQCLQVNPHDRPIAADLINHPFVKRPVSS 560
            L CL+VNP +RP AA+L+NHPFV  P+ S
Sbjct: 1887 LTCLKVNPEERPTAAELLNHPFVNMPLPS 1883

BLAST of CmoCh15G007330 vs. ExPASy Swiss-Prot
Match: A9SY39 (Mitogen-activated protein kinase kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MEKK1a PE=2 SV=2)

HSP 1 Score: 269.6 bits (688), Expect = 8.0e-71
Identity = 148/290 (51.03%), Postives = 195/290 (67.24%), Query Frame = 0

Query: 265 SSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSFGSVSEAISEDGIFFALKEVSLLNE 324
           SS ST    ++ P    +P   +W + G  LG G+FGSV E ++ +G FFA+KEV+L +E
Sbjct: 406 SSVSTESSPSVIPK---RPPWDTWAK-GEFLGSGTFGSVYEGVARNGTFFAVKEVNLADE 465

Query: 325 DSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYGTHSVLPFSDGSKLYIFLELVSQGS 384
              GRQ++ QL++     EI+LLS+ +H NIVQY GT          KLYIFLEL+++GS
Sbjct: 466 GKLGRQAVKQLER-----EIALLSDIQHPNIVQYLGTERT-----EDKLYIFLELLNKGS 525

Query: 385 LLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHRDIKCANILVDVNGSVKLADFGLA 444
           L NLY++  L    + AYT QIL+GLKYLH+R ++HRDIKCANILVD NG VKLADFG+A
Sbjct: 526 LANLYRKYGLFYEQIKAYTEQILTGLKYLHDRKIIHRDIKCANILVDTNGVVKLADFGMA 585

Query: 445 K-ATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADIWSLGCTVLEMLTRKLPYSEVESP 504
           K   KF   KS  G+A WMAPEVV+ K Q Y   ADIWSLGCTVLEM T   P+ E+E  
Sbjct: 586 KQVEKFGFAKSFVGSAHWMAPEVVDPK-QQYNFAADIWSLGCTVLEMATEGPPFGELEF- 645

Query: 505 MQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHDRPIAADLINHPFV 554
           +   ++IG+G+AP++P+ L  + +DFI QCLQV+   RP    L+ HPF+
Sbjct: 646 IAVFWKIGRGEAPLIPDDLEDELKDFIAQCLQVDASKRPTCDMLLAHPFI 679

BLAST of CmoCh15G007330 vs. ExPASy TrEMBL
Match: A0A6J1FHD2 (mitogen-activated protein kinase kinase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445390 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 564/574 (98.26%), Postives = 564/574 (98.26%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM
Sbjct: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD
Sbjct: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120

Query: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG 180
           SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG
Sbjct: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG 180

Query: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
           INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN
Sbjct: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240

Query: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300
           IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF
Sbjct: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300

Query: 301 GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYG 360
           GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ     EISLLSEFEHENIVQYYG
Sbjct: 301 GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ-----EISLLSEFEHENIVQYYG 360

Query: 361 THSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420
           TH     SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH
Sbjct: 361 TH-----SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420

Query: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480
           RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI
Sbjct: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480

Query: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540
           WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD
Sbjct: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540

Query: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI
Sbjct: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 564

BLAST of CmoCh15G007330 vs. ExPASy TrEMBL
Match: A0A6J1JRP4 (mitogen-activated protein kinase kinase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111489185 PE=4 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.0e-307
Identity = 550/574 (95.82%), Postives = 554/574 (96.52%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAY+ SSFDDSS SLYTRSM
Sbjct: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYSYSSFDDSSRSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD
Sbjct: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120

Query: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG 180
           SPKTEEISK KED+ENEDELCRRVKDSVRISDEMSTLKTELPELNER M TVTGRSPRSG
Sbjct: 121 SPKTEEISKGKEDKENEDELCRRVKDSVRISDEMSTLKTELPELNERRMVTVTGRSPRSG 180

Query: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
           INGARPPLLKPPP MRLP FDNAFSTWDILKAFAPL DDKKQDEVEERVEPLVEREVEEN
Sbjct: 181 INGARPPLLKPPPSMRLPVFDNAFSTWDILKAFAPLVDDKKQDEVEERVEPLVEREVEEN 240

Query: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300
           IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMR KPIITSWVETGRLLGRGSF
Sbjct: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRSKPIITSWVETGRLLGRGSF 300

Query: 301 GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYG 360
           GSVSEA+SEDGIFFALKEVSLL+EDSQGRQSI QLQQ     EISLLSEFEHENIVQYYG
Sbjct: 301 GSVSEALSEDGIFFALKEVSLLDEDSQGRQSILQLQQ-----EISLLSEFEHENIVQYYG 360

Query: 361 THSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420
           TH     SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH
Sbjct: 361 TH-----SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420

Query: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480
           RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI
Sbjct: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480

Query: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540
           WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD
Sbjct: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540

Query: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI
Sbjct: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 564

BLAST of CmoCh15G007330 vs. ExPASy TrEMBL
Match: A0A1S3CCL9 (mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC103499270 PE=4 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.6e-263
Identity = 484/579 (83.59%), Postives = 515/579 (88.95%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           M+ LPQFFSPR + K MDPRKI GKPKL RLNAAKNIDYDAA +SSS +DSSGSLYTRSM
Sbjct: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           E  +PSSFRIKGLDGEVDLICRTLGL GPDDFAIPMEAWEARKVRSSS++LPRSRLY +D
Sbjct: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMD 120

Query: 121 -SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRS 180
            SPKTEEISK+KED+E +DELCRRVKDSVRIS E+S  KTE  ELNER M T TG S RS
Sbjct: 121 TSPKTEEISKDKEDKEIQDELCRRVKDSVRISVELSKTKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLV----ER 240
           GINGARPPLLKPPP MRLP+FDNA STWDILK FAPL +D+ Q+EV ERVEPLV    E 
Sbjct: 181 GINGARPPLLKPPPSMRLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240

Query: 241 EVEENIVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLL 300
           E E N VRP E+A+LI SWGSFTTSNDDDSSSSTTEPANISPNMRG PIITSWV  GRLL
Sbjct: 241 EGEGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWV-LGRLL 300

Query: 301 GRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENI 360
           GRGSFGSV EAISEDG FFALKEVSLL+EDSQGRQSI+QLQQ     EI+LLSEFEHENI
Sbjct: 301 GRGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQ-----EIALLSEFEHENI 360

Query: 361 VQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHE 420
           VQYYGTH     SDGSKLYIFLELVSQGSL++LYQRT+L+DSIVSAYTRQILSGLKYLHE
Sbjct: 361 VQYYGTH-----SDGSKLYIFLELVSQGSLMSLYQRTSLMDSIVSAYTRQILSGLKYLHE 420

Query: 421 RNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYG 480
           RNV+HRDIKCANILVDVNGSVKLADFGLAKATK NDVKSCKGTA+WMAPEVVNGK QGYG
Sbjct: 421 RNVIHRDIKCANILVDVNGSVKLADFGLAKATKLNDVKSCKGTAYWMAPEVVNGKSQGYG 480

Query: 481 LPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQ 540
           LPADIWSLGCTVLEMLTRKLPYSE+ES MQAL+RIGKGK P VPESLPKDAQDFILQCLQ
Sbjct: 481 LPADIWSLGCTVLEMLTRKLPYSELESHMQALYRIGKGKPPAVPESLPKDAQDFILQCLQ 540

Query: 541 VNPHDRPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           VNP DRP AADL+NH FVKRPVSSLS SASPYN+PGRRI
Sbjct: 541 VNPKDRPTAADLLNHSFVKRPVSSLSGSASPYNRPGRRI 568

BLAST of CmoCh15G007330 vs. ExPASy TrEMBL
Match: A0A0A0KTG3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G385380 PE=4 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.7e-262
Identity = 482/577 (83.54%), Postives = 514/577 (89.08%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           M+ LPQFFSPR + K MDPRKI GKPKL RLNAAKNIDYDA  +SSS +DSSGSLYTRSM
Sbjct: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           E  +PSSFRIKGLDGEVDLICRTLGL GPDDFAIPMEAWEARKVRSSS++LPRSRLYP+D
Sbjct: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120

Query: 121 -SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRS 180
            SPKTEEIS++KED+E +DELCRRVKDSVRIS ++S  KTE  ELNER M T TG S RS
Sbjct: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPL--VEREV 240
           GINGARPPLLKPPP MRLP+FDNA+STWDILK FAPL +D+ Q+EV ERVEPL  VE E 
Sbjct: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240

Query: 241 EENIVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGR 300
           E N VRP E+A+LI SWGSFTTSNDDDSSSSTTEPANISPNMR  PIITSWV  GRLLGR
Sbjct: 241 EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWV-LGRLLGR 300

Query: 301 GSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQ 360
           GSFGSV EAISEDG FFALKEVSLL+EDSQGRQSI+QLQQ     EI+LLSEFEHENIVQ
Sbjct: 301 GSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQ-----EIALLSEFEHENIVQ 360

Query: 361 YYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERN 420
           YYGTH     SDGSKLYIFLELVSQGSL++LYQRT+L+DSIVSAYTRQILSGLKYLHERN
Sbjct: 361 YYGTH-----SDGSKLYIFLELVSQGSLMSLYQRTSLMDSIVSAYTRQILSGLKYLHERN 420

Query: 421 VVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLP 480
           V+HRDIKCANILVDVNGSVKLADFGLAKATK NDVKSCKGTA+WMAPEVVNGKGQGYGLP
Sbjct: 421 VIHRDIKCANILVDVNGSVKLADFGLAKATKLNDVKSCKGTAYWMAPEVVNGKGQGYGLP 480

Query: 481 ADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVN 540
           ADIWSLGCTVLEMLTRKLPYSE ES MQALFRIGKGK P VPESLPKDAQDFILQCLQVN
Sbjct: 481 ADIWSLGCTVLEMLTRKLPYSEFESHMQALFRIGKGKPPAVPESLPKDAQDFILQCLQVN 540

Query: 541 PHDRPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           P DRP AADL+NH FVKRPVSSLS  ASPYN+PGRRI
Sbjct: 541 PKDRPTAADLLNHSFVKRPVSSLSGLASPYNRPGRRI 566

BLAST of CmoCh15G007330 vs. ExPASy TrEMBL
Match: A0A5D3DRZ7 (Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G00830 PE=4 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 6.4e-257
Identity = 474/563 (84.19%), Postives = 503/563 (89.34%), Query Frame = 0

Query: 17  MDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSMETFNPSSFRIKGLDGE 76
           MDPRKI GKPKL RLNAAKNIDYDAA +SSS +DSSGSLYTRSME  +PSSFRIKGLDGE
Sbjct: 1   MDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 60

Query: 77  VDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD-SPKTEEISKEKEDEE 136
           VDLICRTLGL GPDDFAIPMEAWEARKVRSSS++LPRSRLY +D SPKTEEISK+KED+E
Sbjct: 61  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMDTSPKTEEISKDKEDKE 120

Query: 137 NEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSGINGARPPLLKPPPCM 196
            +DELCRRVKDSVRIS E+S  KTE  ELNER M T TG S RSGINGARPPLLKPPP M
Sbjct: 121 IQDELCRRVKDSVRISVELSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 180

Query: 197 RLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLV----EREVEENIVRPEESAALI 256
           RLP+FDNA STWDILK FAPL +D+ Q+EV ERVEPLV    E E E N VRP E+A+LI
Sbjct: 181 RLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEGEGNTVRPVENASLI 240

Query: 257 ESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSFGSVSEAISEDG 316
            SWGSFTTSNDDDSSSSTTEPANISPNMRG PIITSWV  GRLLGRGSFGSV EAISEDG
Sbjct: 241 GSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWV-LGRLLGRGSFGSVYEAISEDG 300

Query: 317 IFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYGTHSVLPFSDGS 376
            FFALKEVSLL+EDSQGRQSI+QLQQ     EI+LLSEFEHENIVQYYGTH     SDGS
Sbjct: 301 TFFALKEVSLLDEDSQGRQSIYQLQQ-----EIALLSEFEHENIVQYYGTH-----SDGS 360

Query: 377 KLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHRDIKCANILVD 436
           KLYIFLELVSQGSL++LYQRT+L+DSIVSAYTRQILSGLKYLHERNV+HRDIKCANILVD
Sbjct: 361 KLYIFLELVSQGSLMSLYQRTSLMDSIVSAYTRQILSGLKYLHERNVIHRDIKCANILVD 420

Query: 437 VNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADIWSLGCTVLEML 496
           VNGSVKLADFGLAKATK NDVKSCKGTA+WMAPEVVNGK QGYGLPADIWSLGCTVLEML
Sbjct: 421 VNGSVKLADFGLAKATKLNDVKSCKGTAYWMAPEVVNGKSQGYGLPADIWSLGCTVLEML 480

Query: 497 TRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHDRPIAADLINHP 556
           TRKLPYSE+ES MQAL+RIGKGK P VPESLPKDAQDFILQCLQVNP DRP AADL+NH 
Sbjct: 481 TRKLPYSELESHMQALYRIGKGKPPAVPESLPKDAQDFILQCLQVNPKDRPTAADLLNHS 540

Query: 557 FVKRPVSSLSVSASPYNKPGRRI 575
           FVKRPVSSLS SASPYN+PGRRI
Sbjct: 541 FVKRPVSSLSGSASPYNRPGRRI 552

BLAST of CmoCh15G007330 vs. NCBI nr
Match: XP_022939504.1 (mitogen-activated protein kinase kinase kinase 1-like [Cucurbita moschata] >KAG7016523.1 Mitogen-activated protein kinase kinase kinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 564/574 (98.26%), Postives = 564/574 (98.26%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM
Sbjct: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD
Sbjct: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120

Query: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG 180
           SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG
Sbjct: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG 180

Query: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
           INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN
Sbjct: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240

Query: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300
           IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF
Sbjct: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300

Query: 301 GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYG 360
           GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ     EISLLSEFEHENIVQYYG
Sbjct: 301 GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ-----EISLLSEFEHENIVQYYG 360

Query: 361 THSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420
           TH     SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH
Sbjct: 361 TH-----SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420

Query: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480
           RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI
Sbjct: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480

Query: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540
           WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD
Sbjct: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540

Query: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI
Sbjct: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 564

BLAST of CmoCh15G007330 vs. NCBI nr
Match: XP_023551070.1 (mitogen-activated protein kinase kinase kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 557/574 (97.04%), Postives = 562/574 (97.91%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAY+SSSFDDSSGSLYTRSM
Sbjct: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYSSSSFDDSSGSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSR+YPVD
Sbjct: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRMYPVD 120

Query: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG 180
           SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPEL+ER MDTVTGRSPRSG
Sbjct: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELSERRMDTVTGRSPRSG 180

Query: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
           INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEER+EPLVEREVEEN
Sbjct: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERLEPLVEREVEEN 240

Query: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300
           IVRP ESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF
Sbjct: 241 IVRPVESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300

Query: 301 GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYG 360
           GSVSEAISEDGIFFALKEVSLL+EDSQGRQSIFQLQQ     EISLLSEFEHENIVQYYG
Sbjct: 301 GSVSEAISEDGIFFALKEVSLLDEDSQGRQSIFQLQQ-----EISLLSEFEHENIVQYYG 360

Query: 361 THSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420
           TH     SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH
Sbjct: 361 TH-----SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420

Query: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480
           RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI
Sbjct: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480

Query: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540
           WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD
Sbjct: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540

Query: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI
Sbjct: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 564

BLAST of CmoCh15G007330 vs. NCBI nr
Match: XP_022993052.1 (mitogen-activated protein kinase kinase kinase 1-like [Cucurbita maxima])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.2e-307
Identity = 550/574 (95.82%), Postives = 554/574 (96.52%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAY+ SSFDDSS SLYTRSM
Sbjct: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYSYSSFDDSSRSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD
Sbjct: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120

Query: 121 SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSG 180
           SPKTEEISK KED+ENEDELCRRVKDSVRISDEMSTLKTELPELNER M TVTGRSPRSG
Sbjct: 121 SPKTEEISKGKEDKENEDELCRRVKDSVRISDEMSTLKTELPELNERRMVTVTGRSPRSG 180

Query: 181 INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
           INGARPPLLKPPP MRLP FDNAFSTWDILKAFAPL DDKKQDEVEERVEPLVEREVEEN
Sbjct: 181 INGARPPLLKPPPSMRLPVFDNAFSTWDILKAFAPLVDDKKQDEVEERVEPLVEREVEEN 240

Query: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSF 300
           IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMR KPIITSWVETGRLLGRGSF
Sbjct: 241 IVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRSKPIITSWVETGRLLGRGSF 300

Query: 301 GSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYG 360
           GSVSEA+SEDGIFFALKEVSLL+EDSQGRQSI QLQQ     EISLLSEFEHENIVQYYG
Sbjct: 301 GSVSEALSEDGIFFALKEVSLLDEDSQGRQSILQLQQ-----EISLLSEFEHENIVQYYG 360

Query: 361 THSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420
           TH     SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH
Sbjct: 361 TH-----SDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVH 420

Query: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480
           RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI
Sbjct: 421 RDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADI 480

Query: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540
           WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD
Sbjct: 481 WSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPHD 540

Query: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI
Sbjct: 541 RPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 564

BLAST of CmoCh15G007330 vs. NCBI nr
Match: KAG6579001.1 (Mitogen-activated protein kinase kinase kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 988.8 bits (2555), Expect = 2.0e-284
Identity = 510/521 (97.89%), Postives = 511/521 (98.08%), Query Frame = 0

Query: 11  RKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSMETFNPSSFRI 70
           RKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSMETFNPSSFRI
Sbjct: 68  RKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSMETFNPSSFRI 127

Query: 71  KGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVDSPKTEEISKE 130
           KGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVDSPKTEEISKE
Sbjct: 128 KGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVDSPKTEEISKE 187

Query: 131 KEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSGINGARPPLLK 190
           KEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSGINGARPPLLK
Sbjct: 188 KEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRSGINGARPPLLK 247

Query: 191 PPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEENIVRPEESAAL 250
           PPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEV+ERVEPLVEREVEENIVRPEESAAL
Sbjct: 248 PPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVKERVEPLVEREVEENIVRPEESAAL 307

Query: 251 IESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSFGSVSEAISED 310
           IESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSFGSVSEAISED
Sbjct: 308 IESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGSFGSVSEAISED 367

Query: 311 GIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYGTHSVLPFSDG 370
           GIFFALKEVSLLNEDSQGRQSIFQLQQ     EISLLSEFEHENIVQYYGTH     SDG
Sbjct: 368 GIFFALKEVSLLNEDSQGRQSIFQLQQ-----EISLLSEFEHENIVQYYGTH-----SDG 427

Query: 371 SKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHRDIKCANILV 430
           SKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHRDIKCANILV
Sbjct: 428 SKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHRDIKCANILV 487

Query: 431 DVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADIWSLGCTVLEM 490
           DVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADIWSLGCTVLEM
Sbjct: 488 DVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPADIWSLGCTVLEM 547

Query: 491 LTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFIL 532
           LTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFIL
Sbjct: 548 LTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFIL 578

BLAST of CmoCh15G007330 vs. NCBI nr
Match: XP_038874370.1 (mitogen-activated protein kinase kinase kinase 1-like [Benincasa hispida])

HSP 1 Score: 944.5 bits (2440), Expect = 4.3e-271
Identity = 493/575 (85.74%), Postives = 523/575 (90.96%), Query Frame = 0

Query: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYTSSSFDDSSGSLYTRSM 60
           MH LPQFFSPRK+ K MDPRKIAGKPKL RLNAAKNIDYDAA +SSS DDSSGSLYTRSM
Sbjct: 1   MHRLPQFFSPRKKGKPMDPRKIAGKPKLGRLNAAKNIDYDAASSSSSLDDSSGSLYTRSM 60

Query: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120
           ET +PSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSS++LPRSR+YP+D
Sbjct: 61  ETPDPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSELLPRSRMYPMD 120

Query: 121 -SPKTEEISKEKEDEENEDELCRRVKDSVRISDEMSTLKTELPELNERCMDTVTGRSPRS 180
            SPKTEEI+K+KED+E +DELCRRVKDSVRIS E+ST KTE  ELNER M T TG S RS
Sbjct: 121 SSPKTEEINKDKEDKEIQDELCRRVKDSVRISVELSTPKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEE 240
           GINGARPPLLKPPP MRLP+ DN  STWDILK+FAPL DD+ Q+EV ERVEPLV+RE E 
Sbjct: 181 GINGARPPLLKPPPSMRLPNLDNTCSTWDILKSFAPLVDDEHQEEVGERVEPLVDREGEG 240

Query: 241 NIVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRGKPIITSWVETGRLLGRGS 300
           NIVRP E+A LI SWGSFTTSNDDDSSSSTTEPANISPNMRG PIITSWV  GRLLGRGS
Sbjct: 241 NIVRPVENALLIGSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWV-VGRLLGRGS 300

Query: 301 FGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYY 360
           FGSV EAIS+DG FFALKEVSLL+EDSQGRQSI+QLQQ     EI+LLSEFEHENIVQYY
Sbjct: 301 FGSVYEAISQDGTFFALKEVSLLDEDSQGRQSIYQLQQ-----EIALLSEFEHENIVQYY 360

Query: 361 GTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVV 420
           GTH     SDGSKLYIFLELVSQGSL++LYQRT+L+DSIVSAYTRQILSGLKYLHERNVV
Sbjct: 361 GTH-----SDGSKLYIFLELVSQGSLMSLYQRTSLMDSIVSAYTRQILSGLKYLHERNVV 420

Query: 421 HRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKGQGYGLPAD 480
           HRDIKCANILVDVNGSVKLADFGLAKATK NDVKSCKGTA+WMAPEVVNGKGQGYGLPAD
Sbjct: 421 HRDIKCANILVDVNGSVKLADFGLAKATKLNDVKSCKGTAYWMAPEVVNGKGQGYGLPAD 480

Query: 481 IWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQCLQVNPH 540
           IWSLGCTVLEMLTRKLPYSE+ES MQALFRIGKGKAP VPESLPKDAQDFILQCLQVNP 
Sbjct: 481 IWSLGCTVLEMLTRKLPYSELESHMQALFRIGKGKAPAVPESLPKDAQDFILQCLQVNPK 540

Query: 541 DRPIAADLINHPFVKRPVSSLSVSASPYNKPGRRI 575
           DRP AADL+NH FVKRPVSSLS SASPYN+PGRRI
Sbjct: 541 DRPTAADLLNHSFVKRPVSSLSGSASPYNRPGRRI 564

BLAST of CmoCh15G007330 vs. TAIR 10
Match: AT4G08500.1 (MAPK/ERK kinase kinase 1 )

HSP 1 Score: 475.7 bits (1223), Expect = 5.2e-134
Identity = 295/594 (49.66%), Postives = 377/594 (63.47%), Query Frame = 0

Query: 27  KLYRLNAAKNIDYDAAYTSSSFDD----SSGSLYTRSMETFNPSSFRIKGLDGEVDLICR 86
           +L R +AA+NI+YDAA  SSS  +    S+ SL TRS+E   P+SFRI G  GE+D I R
Sbjct: 26  RLERRDAARNINYDAASCSSSSAEDLSVSTSSLMTRSLEFPEPTSFRIGGGVGEMDRIYR 85

Query: 87  TLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVDSPKTEEISKEKED--EENEDEL 146
           +LG+ GPDD AI  +AWEA K RSSSD++ R + + +D  + +++S+E         D +
Sbjct: 86  SLGVSGPDDLAISFDAWEACKKRSSSDVVNRFKSFDLDKVRDQDLSEEGPSGVVVGSDSM 145

Query: 147 CRRVK-DSVRISDEMSTLKTELPEL-----------------NERCMD------------ 206
             +V+   +  +     + TEL E+                 N R M+            
Sbjct: 146 NHKVQGQDLSEAGPSGGIVTELSEIGNLITPVDRLVADGVVENRRVMERTPTIVKSKGYL 205

Query: 207 -----TVTGRSPRSGINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKK---- 266
                   G     GI G RPP+LKPPP M+ P  D+  S+WD L  FAP E  K+    
Sbjct: 206 VPNNVVAVGVGVGGGIKGLRPPVLKPPPAMKRPPIDHRGSSWDFLTHFAPSETVKRPSSS 265

Query: 267 ----QDEVEERVEPLVEREVEENIVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISP 326
               +D  +E      E E EE   R  +     +   SFTT N+ DSSS+ +  + I P
Sbjct: 266 SSSSEDGCDEEEGKEEEAEAEEMGARFIQLGDTADETCSFTT-NEGDSSSTVSNTSPIYP 325

Query: 327 NMRGKPIITSWVETGRLLGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ 386
           +  G  IITSW + G+LLGRGSFGSV E IS DG FFA+KEVSLL++ SQ ++ I QL+ 
Sbjct: 326 D--GGAIITSW-QKGQLLGRGSFGSVYEGISGDGDFFAVKEVSLLDQGSQAQECIQQLEG 385

Query: 387 VLHLFEISLLSEFEHENIVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDS 446
                EI LLS+ +H+NIV+Y GT       DGS LYIFLELV+QGSLL LYQR  L DS
Sbjct: 386 -----EIKLLSQLQHQNIVRYRGT-----AKDGSNLYIFLELVTQGSLLKLYQRYQLRDS 445

Query: 447 IVSAYTRQILSGLKYLHERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKG 506
           +VS YTRQIL GLKYLH++  +HRDIKCANILVD NG+VKLADFGLAK +KFND+KSCKG
Sbjct: 446 VVSLYTRQILDGLKYLHDKGFIHRDIKCANILVDANGAVKLADFGLAKVSKFNDIKSCKG 505

Query: 507 TAFWMAPEVVNGK-GQGYGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAP 566
           T FWMAPEV+N K   GYG PADIWSLGCTVLEM T ++PYS++E P+QALFRIG+G  P
Sbjct: 506 TPFWMAPEVINRKDSDGYGSPADIWSLGCTVLEMCTGQIPYSDLE-PVQALFRIGRGTLP 565

BLAST of CmoCh15G007330 vs. TAIR 10
Match: AT4G08480.1 (mitogen-activated protein kinase kinase kinase 9 )

HSP 1 Score: 391.7 bits (1005), Expect = 9.9e-109
Identity = 279/687 (40.61%), Postives = 374/687 (54.44%), Query Frame = 0

Query: 9   SPRKRSKAMDPRKIAGKPKLYRLNAA-KNIDYDAAYTSSSFDDSSGSLYTRSMETFNPSS 68
           SP +R     P +     +L R +A  +NIDYDA       +DSS  L TRS++  N +S
Sbjct: 110 SPGRRGDKTSPAR-----RLDRSDAVRRNIDYDAG------EDSSSLLITRSLDFPNRTS 169

Query: 69  FRIKGL-DGEVDLICRTLGLDGPDDFAIPMEAWEAR--KVRSSSDILPRSRLYPVD---- 128
           FR+ G+ DGE+D I + +G+ GP+DFAI  +AW+AR    RSSSD++ + +   +D    
Sbjct: 170 FRVDGVDDGEIDRIYQYIGVSGPEDFAISSDAWKARMEHERSSSDVVNKLKSLDLDSREA 229

Query: 129 ---------------------------------------SPKTEEISKEKEDE------- 188
                                                  S K E ++  ++ E       
Sbjct: 230 GPSGGVVASSSMNHKFQGHDLSEAGSIGVVVASNFTLSESNKIENLNSLRDKEIVDGDMV 289

Query: 189 ENEDELCRRV------------KDSVRISDEMSTLK---TELPELNERCMD--TVTGRS- 248
           EN   + R+              D V +   +  ++     +P  ++  +D  TV  +S 
Sbjct: 290 ENRCGIERKPTILVKSRGYLVHNDDVGVGGGIKGVRPPVLNVPRADKEVVDGGTVESKSG 349

Query: 249 ----------------------------------------------PRSGINGARPPLLK 308
                                                          R GI G RP +LK
Sbjct: 350 IEWKPTILVKSKGYLVSNDGGIKGVTSPVLNLRPTDKEVVDSGTVENRRGIKGVRPSVLK 409

Query: 309 PPPCMRLPDFDNAFSTWDILKAFAP-LEDDKKQDEVEERVEPLVEREVEENIVRPEESAA 368
           PPP M+LP  D   S+WDIL  FAP  E  ++            E E E++ V  EE+  
Sbjct: 410 PPPVMKLPPVDLPGSSWDILTHFAPDSEIVRRPSSSSSSENGCDEEEAEDDKVEKEETGD 469

Query: 369 LI-------ESWGSFTTSNDDDSSS-STTEPANISPNMRGKPIITSWVETGRLLGRGSFG 428
           +        +   SFTT+  D SS+ S T P  +S    G  I TSW + G+LL +GSFG
Sbjct: 470 MFIQLEDTTDEACSFTTNEGDSSSTVSNTSPICVS----GGSINTSW-QKGQLLRQGSFG 529

Query: 429 SVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHENIVQYYGT 488
           SV EAISEDG FFA+KEVSLL++ SQ ++ I QL+      EI+LLS+ EH+NI++Y GT
Sbjct: 530 SVYEAISEDGDFFAVKEVSLLDQGSQAQECIQQLEG-----EIALLSQLEHQNILRYRGT 589

Query: 489 HSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLHERNVVHR 548
                  DGS LYIFLELV+QGSLL LY+R  + DS++S YT+QIL GLKYLH +  +HR
Sbjct: 590 D-----KDGSNLYIFLELVTQGSLLELYRRYQIRDSLISLYTKQILDGLKYLHHKGFIHR 649

Query: 549 DIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGK-GQGYGLPADI 567
           DIKCA ILVD NG+VKLADFGLAK +K ND+KS K T FWMAPEV+N K   GY  PADI
Sbjct: 650 DIKCATILVDANGTVKLADFGLAKVSKLNDIKSRKETLFWMAPEVINRKDNDGYRSPADI 709

BLAST of CmoCh15G007330 vs. TAIR 10
Match: AT4G08470.1 (MAPK/ERK kinase kinase 3 )

HSP 1 Score: 325.5 bits (833), Expect = 8.7e-89
Identity = 170/277 (61.37%), Postives = 215/277 (77.62%), Query Frame = 0

Query: 278 NMRGKPIITSWVETGRLLGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQ 337
           N R    ITSW++ G+LLGRGS+ SV EAISEDG FFA+KEVSLL++  Q ++ I QL+ 
Sbjct: 293 NFRKPEDITSWLK-GQLLGRGSYASVYEAISEDGDFFAVKEVSLLDKGIQAQECIQQLEG 352

Query: 338 VLHLFEISLLSEFEHENIVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDS 397
                EI+LLS+ +H+NIV+Y GT       D SKLYIFLELV+QGS+  LY+R  L  +
Sbjct: 353 -----EIALLSQLQHQNIVRYRGT-----AKDVSKLYIFLELVTQGSVQKLYERYQLSYT 412

Query: 398 IVSAYTRQILSGLKYLHERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKG 457
           +VS YTRQIL+GL YLH++  VHRDIKCAN+LVD NG+VKLADFGLA+A+KFND+ SCKG
Sbjct: 413 VVSLYTRQILAGLNYLHDKGFVHRDIKCANMLVDANGTVKLADFGLAEASKFNDIMSCKG 472

Query: 458 TAFWMAPEVVNGK-GQGYGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAP 517
           T FWMAPEV+N K   G G PADIWSLGCTVLEM T ++PYS+++ P+QA F+IG+G  P
Sbjct: 473 TLFWMAPEVINRKDSDGNGSPADIWSLGCTVLEMCTGQIPYSDLK-PIQAAFKIGRGTLP 532

Query: 518 VVPESLPKDAQDFILQCLQVNPHDRPIAADLINHPFV 554
            VP++L  DA+ FIL CL+VNP +RP AA+L++HPFV
Sbjct: 533 DVPDTLSLDARHFILTCLKVNPEERPTAAELLHHPFV 557


HSP 2 Score: 50.4 bits (119), Expect = 5.4e-06
Identity = 43/140 (30.71%), Postives = 66/140 (47.14%), Query Frame = 0

Query: 180 GINGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEE 239
           GI   RPP+++PPP  +LP  D   S+WD L  FAP +  K+Q           +   ++
Sbjct: 30  GIKAVRPPIIQPPPGRKLPLIDFPGSSWDFLTYFAPSKTVKRQSSSSS------DNTSDK 89

Query: 240 NIVRPEESAALIESWGSFT------TSNDDDSSSSTTEPANISPNMRGK-PIITSWVETG 299
             V  EE+  +    G          +N+ DSSS+    + ISP+   +  I+ SW++  
Sbjct: 90  EEVETEETRGMFVQLGDTAHEACPFATNEADSSSTV---SIISPSYASRGSIVPSWLKR- 149

Query: 300 RLLGRGSFGSVSEAISEDGI 313
           + LGR S G V E  S   +
Sbjct: 150 KFLGRVSLGFVYEGSSGSSV 159

BLAST of CmoCh15G007330 vs. TAIR 10
Match: AT4G12020.2 (protein kinase family protein )

HSP 1 Score: 316.6 bits (810), Expect = 4.1e-86
Identity = 199/389 (51.16%), Postives = 242/389 (62.21%), Query Frame = 0

Query: 181  INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
            I G RPP+L+  P M+L       STWD +  FAP          E    P    E  E 
Sbjct: 1527 IKGLRPPVLQLQPAMKLSHIPRG-STWDFVTHFAP---------PETVAPPSSSSEAREE 1586

Query: 241  IVRPEESAALIESWG-----SFTTSNDDDSSS-STTEPANISPNMRGKPIITSWVETGRL 300
             V  EE+ A+    G     SFT +  D S + S T P   S        IT W + G+L
Sbjct: 1587 EVETEETGAMFIPLGDKETCSFTVNKGDSSRTISNTSPIYASEG----SFITCW-QKGQL 1646

Query: 301  LGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHEN 360
            LGRGS GSV E IS DG FFA KEVSLL++ SQ  + I Q++       I+LLS+ +H+N
Sbjct: 1647 LGRGSLGSVYEGISADGDFFAFKEVSLLDQGSQAHEWIQQVEG-----GIALLSQLQHQN 1706

Query: 361  IVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLH 420
            IV+Y GT       D S LYIFLELV+QGSL  LYQR  L DS+VS YTRQIL GLKYLH
Sbjct: 1707 IVRYRGT-----TKDESNLYIFLELVTQGSLRKLYQRNQLGDSVVSLYTRQILDGLKYLH 1766

Query: 421  ERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAF--WMAPEVV-NGKG 480
            ++  +HR+IKCAN+LVD NG+VKLADFGLAK      V S   T +  WMAPEV+ N K 
Sbjct: 1767 DKGFIHRNIKCANVLVDANGTVKLADFGLAK------VMSLWRTPYWNWMAPEVILNPKD 1826

Query: 481  -QGYGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFI 540
              GYG PADIWSLGCTVLEMLT ++PYS++E    AL+ IG GK P +P+ L  DA+DFI
Sbjct: 1827 YDGYGTPADIWSLGCTVLEMLTGQIPYSDLEIG-TALYNIGTGKLPKIPDILSLDARDFI 1883

Query: 541  LQCLQVNPHDRPIAADLINHPFVKRPVSS 560
            L CL+VNP +RP AA+L+NHPFV  P+ S
Sbjct: 1887 LTCLKVNPEERPTAAELLNHPFVNMPLPS 1883

BLAST of CmoCh15G007330 vs. TAIR 10
Match: AT4G12020.3 (protein kinase family protein )

HSP 1 Score: 304.3 bits (778), Expect = 2.1e-82
Identity = 190/386 (49.22%), Postives = 233/386 (60.36%), Query Frame = 0

Query: 181  INGARPPLLKPPPCMRLPDFDNAFSTWDILKAFAPLEDDKKQDEVEERVEPLVEREVEEN 240
            I G RPP+L+  P M+L       STWD +  FAP          E    P    E  E 
Sbjct: 1527 IKGLRPPVLQLQPAMKLSHIPRG-STWDFVTHFAP---------PETVAPPSSSSEAREE 1586

Query: 241  IVRPEESAALIESWG-----SFTTSNDDDSSS-STTEPANISPNMRGKPIITSWVETGRL 300
             V  EE+ A+    G     SFT +  D S + S T P   S        IT W + G+L
Sbjct: 1587 EVETEETGAMFIPLGDKETCSFTVNKGDSSRTISNTSPIYASEG----SFITCW-QKGQL 1646

Query: 301  LGRGSFGSVSEAISEDGIFFALKEVSLLNEDSQGRQSIFQLQQVLHLFEISLLSEFEHEN 360
            LGRGS GSV E IS DG FFA KEVSLL++ SQ  + I Q++       I+LLS+ +H+N
Sbjct: 1647 LGRGSLGSVYEGISADGDFFAFKEVSLLDQGSQAHEWIQQVEG-----GIALLSQLQHQN 1706

Query: 361  IVQYYGTHSVLPFSDGSKLYIFLELVSQGSLLNLYQRTNLLDSIVSAYTRQILSGLKYLH 420
            IV+Y GT       D S LYIFLELV+QGSL  LYQR  L DS+VS YTRQIL GLKYLH
Sbjct: 1707 IVRYRGT-----TKDESNLYIFLELVTQGSLRKLYQRNQLGDSVVSLYTRQILDGLKYLH 1766

Query: 421  ERNVVHRDIKCANILVDVNGSVKLADFGLAKATKFNDVKSCKGTAFWMAPEVVNGKG-QG 480
            ++  +HR+IKCAN+LVD NG+VKLADFGLAK                    ++N K   G
Sbjct: 1767 DKGFIHRNIKCANVLVDANGTVKLADFGLAKV-------------------ILNPKDYDG 1826

Query: 481  YGLPADIWSLGCTVLEMLTRKLPYSEVESPMQALFRIGKGKAPVVPESLPKDAQDFILQC 540
            YG PADIWSLGCTVLEMLT ++PYS++E    AL+ IG GK P +P+ L  DA+DFIL C
Sbjct: 1827 YGTPADIWSLGCTVLEMLTGQIPYSDLEIG-TALYNIGTGKLPKIPDILSLDARDFILTC 1867

Query: 541  LQVNPHDRPIAADLINHPFVKRPVSS 560
            L+VNP +RP AA+L+NHPFV  P+ S
Sbjct: 1887 LKVNPEERPTAAELLNHPFVNMPLPS 1867

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q390087.3e-13349.66Mitogen-activated protein kinase kinase kinase 1 OS=Arabidopsis thaliana OX=3702... [more]
O814721.4e-10740.61Mitogen-activated protein kinase kinase kinase 9 OS=Arabidopsis thaliana OX=3702... [more]
Q9M0T31.2e-8761.37Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Q9SZ675.7e-8551.16Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 ... [more]
A9SY398.0e-7151.03Mitogen-activated protein kinase kinase kinase 1a OS=Physcomitrium patens OX=321... [more]
Match NameE-valueIdentityDescription
A0A6J1FHD20.0e+0098.26mitogen-activated protein kinase kinase kinase 1-like OS=Cucurbita moschata OX=3... [more]
A0A6J1JRP41.0e-30795.82mitogen-activated protein kinase kinase kinase 1-like OS=Cucurbita maxima OX=366... [more]
A0A1S3CCL94.6e-26383.59mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo OX=3656 GN... [more]
A0A0A0KTG31.7e-26283.54Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G385... [more]
A0A5D3DRZ76.4e-25784.19Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
XP_022939504.10.0e+0098.26mitogen-activated protein kinase kinase kinase 1-like [Cucurbita moschata] >KAG7... [more]
XP_023551070.10.0e+0097.04mitogen-activated protein kinase kinase kinase 1-like [Cucurbita pepo subsp. pep... [more]
XP_022993052.12.2e-30795.82mitogen-activated protein kinase kinase kinase 1-like [Cucurbita maxima][more]
KAG6579001.12.0e-28497.89Mitogen-activated protein kinase kinase kinase 1, partial [Cucurbita argyrosperm... [more]
XP_038874370.14.3e-27185.74mitogen-activated protein kinase kinase kinase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G08500.15.2e-13449.66MAPK/ERK kinase kinase 1 [more]
AT4G08480.19.9e-10940.61mitogen-activated protein kinase kinase kinase 9 [more]
AT4G08470.18.7e-8961.37MAPK/ERK kinase kinase 3 [more]
AT4G12020.24.1e-8651.16protein kinase family protein [more]
AT4G12020.32.1e-8249.22protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 412..430
score: 48.67
coord: 377..390
score: 31.71
coord: 478..500
score: 25.21
coord: 522..544
score: 25.03
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 289..553
e-value: 7.2E-82
score: 288.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 290..553
e-value: 1.8E-61
score: 207.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 289..553
score: 47.96162
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 288..569
e-value: 1.3E-73
score: 249.6
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 291..550
e-value: 3.1E-16
score: 57.1
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 286..547
e-value: 1.1E-21
score: 74.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..278
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..139
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..192
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 113..139
NoneNo IPR availablePANTHERPTHR48016:SF33SERINE/THREONINE/DUAL SPECIFICITY PROTEIN KINASE, CATALYTIC DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 24..153
NoneNo IPR availablePANTHERPTHR48016MAP KINASE KINASE KINASE SSK2-RELATED-RELATEDcoord: 24..153
NoneNo IPR availablePANTHERPTHR48016:SF33SERINE/THREONINE/DUAL SPECIFICITY PROTEIN KINASE, CATALYTIC DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 178..563
NoneNo IPR availablePANTHERPTHR48016MAP KINASE KINASE KINASE SSK2-RELATED-RELATEDcoord: 178..563
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 418..430
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 295..317
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 263..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G007330.1CmoCh15G007330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity