CmoCh15G004150 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G004150
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptiondolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A
LocationCmo_Chr15: 1878603 .. 1880790 (-)
RNA-Seq ExpressionCmoCh15G004150
SyntenyCmoCh15G004150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTACGAGATTTTTACCTTCGATTAGCCAAATCAAACTCTGAGGGCTTAAAATTTCCTTCCGTTTCGCCTTGGATTCGAAGGGTTTTAGCGCAAATTTATATAGAAACAGATATGGATTCACGATCCTATGATCGAAAATTGAGAACTGAGTTCAATTTGAATGAGTGTTACGTATCGAGATTAGCTTTTGTTTTTAGATTAATCATTTTTTTTTTATATATATAAAAATTAATTTCATTTCTTCTTTCTTTTTTTCGCCCGAAGAGCCACCTGTCTCCAAAAACAATTTATAATACAATTTGGCGCAGATTGTCAATACGTATTTAAAATATTAATTGTTTCTACTTAATTGTGCTAAATTGCAGTAAATACCCTTCAACTTTATGTTTATTCCAGAAAATGCCCATAATTTTTCTAAAATTTTAATAATATCCTTATCAATTTTTTAAAATTTCATCGTATCTAATAAGTTATTTAATTTAAAAGATTTAATATAATGTATTATATACATTTTAAATTTTATGGACCCACCAAAAATTTAAAACGAAAAAATTTATAGAGTAACCTTAAAATTACAAGTGTAATTATTTCGATAAAATTAGTATTTTTAATTATAGTAAGGTAGAAATGTGTATAGATCACGTGTCAAATGCGGACTGAATTTTTACATTATAAAAAGAAATTATGGCTGAATTTTGGTAGCTTAGCTGCCTAGTCACCGGCTGAGGACTACACGTGGGTCACATGCAAATTCCAGCTCAGCAATAGTAAATGGCAGGGGCAGGGGTTCTTAGTGTTTGCTGTTTTTTTCTCTTCAATTTAGTTCCAATCATTAGGGTTTGTATTTTGTTTTTTTTTTTTAAAAACCAGAATTTTGGATTGGGCTAAAATTTTAGAAAATTGTATTAAAATATAGATTTAAATATAGGAAAAATTAGAAAAAGGAAAAATTAATGAAATATTATTGGGCTGAATGAATTACTGAAGCCCAATAATACCAAAATTAATGGGCTAGGGCCCAAACGTCTGTTTGAGAAGGCTGCATCAAGTAGAAACGAGGTTCTTTTCCGTAGTCTACTGCATCTCGAAGAATTTCGTCGTCGGATCTTCTTTTTGACTTCGTGTTTCTCTCTCGACTGATTAGATCGGAAGGTAAAGTCGTCTTCCTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAGATCGGAAGGTAAAGTCGTCTTTTTTTTTTTTTTTTTTTTTCTCAATTTGATGCAATTGTTTTTTTGTTTTTTTGTTTTTTTGTTTTTTTATCGATCTTTCATGGTTTAGAAGTGGAATTTCGTTTTCTTGGCAAATCTCGATTCTTAAATCTCAAGACTTGCGAGTCGAGCTTCCAAACTGTGCGATTAGGTTATGATGTTTAGTTTTTGAGGAACTTGAAATTTGCGGTTTGCTGCGTTGAAATTTTGATTCATTGTAGGCGATCGAAGAAGCTAATTTCCGCTGTATTGTTTGTTGCCTAGTGCAGATGATTGACGATCAGGAATTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCCTTGGTGATTGCTTACCATTACGTTACGGCTGATCCGAAATATGAAGGCAACTGAGATCTCGTTGATTTAGATGCTTAGATTCATCGGCAAAATGCACTACTGTTAGATGTAGGCCGTTTTTTATATGTTAGGTTTTCCATTTTCTTCTATGAGAATTTTCTCCCGGCAACAACCAATGTAAGCAACTTTTTCATTATGCAATCGTAATTTATTCTCATAAATTTTCATGGTTCTTTGACTGATCTTTTATTAGTTACGTATTTGATCGAATTTCTTCGTACTGCCCATTTTGAGTTCCTTCAATGTTGTCTG

mRNA sequence

ATGTTACGAGATTTTTACCTTCGATTAGCCAAATCAAACTCTGAGGGCTTAAAATTTCCTTCCGTTTCGCCTTGGATTCGAAGGGTTTTAGCGCAAATTTATATAGAAACAGATATGGATTCACGATCCTATGATCGAAAATTGAGAACTGAGTTCAATTTGAATGAGTATCGGAAGATGATTGACGATCAGGAATTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCCTTGGTGATTGCTTACCATTACGTTACGGCTGATCCGAAATATGAAGGCAACTGAGATCTCGTTGATTTAGATGCTTAGATTCATCGGCAAAATGCACTACTGTTAGATGTAGGCCGTTTTTTATATGTTAGGTTTTCCATTTTCTTCTATGAGAATTTTCTCCCGGCAACAACCAATGTAAGCAACTTTTTCATTATGCAATCGTAATTTATTCTCATAAATTTTCATGGTTCTTTGACTGATCTTTTATTAGTTACGTATTTGATCGAATTTCTTCGTACTGCCCATTTTGAGTTCCTTCAATGTTGTCTG

Coding sequence (CDS)

ATGTTACGAGATTTTTACCTTCGATTAGCCAAATCAAACTCTGAGGGCTTAAAATTTCCTTCCGTTTCGCCTTGGATTCGAAGGGTTTTAGCGCAAATTTATATAGAAACAGATATGGATTCACGATCCTATGATCGAAAATTGAGAACTGAGTTCAATTTGAATGAGTATCGGAAGATGATTGACGATCAGGAATTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCCTTGGTGATTGCTTACCATTACGTTACGGCTGATCCGAAATATGAAGGCAACTGA

Protein sequence

MLRDFYLRLAKSNSEGLKFPSVSPWIRRVLAQIYIETDMDSRSYDRKLRTEFNLNEYRKMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Homology
[Close]
BLAST of CmoCh15G004150 vs. ExPASy Swiss-Prot
Match: Q9LHK3 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Arabidopsis thaliana OX=3702 GN=OST4A PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.4e-12
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 95
          MIDDQ+LGF+ANFLGIFIFALVIAYHYVTADPKYE
Sbjct: 1  MIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYE 35

[Close]
BLAST of CmoCh15G004150 vs. ExPASy Swiss-Prot
Match: C7J4U3 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Oryza sativa subsp. japonica OX=39947 GN=OST4A PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-11
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          M DDQ+LGF ANFLGIFIF LVIAYH+V ADPKYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 37

[Close]
BLAST of CmoCh15G004150 vs. ExPASy Swiss-Prot
Match: C7J0R5 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Oryza sativa subsp. japonica OX=39947 GN=OST4B PE=3 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 3.8e-10
Identity = 29/37 (78.38%), Postives = 32/37 (86.49%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          M DDQ+LGF ANFLGIFIF LV+AYH+V AD KYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVMAYHFVMADVKYEGN 37

[Close]
BLAST of CmoCh15G004150 vs. ExPASy Swiss-Prot
Match: Q9SF57 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Arabidopsis thaliana OX=3702 GN=OST4C PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.9e-09
Identity = 28/35 (80.00%), Postives = 31/35 (88.57%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 95
          M DDQ+LGF ANFLGIFIF LVIAYH+V ADPK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFILVIAYHFVMADPKFE 35

[Close]
BLAST of CmoCh15G004150 vs. ExPASy Swiss-Prot
Match: Q8L986 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Arabidopsis thaliana OX=3702 GN=OST4B PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 7.2e-09
Identity = 26/35 (74.29%), Postives = 31/35 (88.57%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 95
          M DDQ+LGF ANFLGIFIF +VIAYH+V A+PK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFIMVIAYHFVVAEPKFE 35

[Close]
BLAST of CmoCh15G004150 vs. ExPASy TrEMBL
Match: A0A5J4ZJS3 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_016341 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.2e-12
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 0 

Query: 55 NEYRKMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          N ++KMIDDQ+LGF ANFLGIFIFALVIAYHYV ADPKYEGN
Sbjct: 44 NRWQKMIDDQDLGFFANFLGIFIFALVIAYHYVVADPKYEGN 85

[Close]
BLAST of CmoCh15G004150 vs. ExPASy TrEMBL
Match: A0A1S3CJC4 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cucumis melo OX=3656 GN=LOC103501634 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 7.2e-12
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]
BLAST of CmoCh15G004150 vs. ExPASy TrEMBL
Match: A0A6J1JUF0 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cucurbita maxima OX=3661 GN=LOC111489813 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 7.2e-12
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]
BLAST of CmoCh15G004150 vs. ExPASy TrEMBL
Match: A0A6J1FGH7 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cucurbita moschata OX=3662 GN=LOC111445281 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 7.2e-12
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]
BLAST of CmoCh15G004150 vs. ExPASy TrEMBL
Match: A0A059A7I3 (Uncharacterized protein (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K03148 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 9.5e-12
Identity = 36/38 (94.74%), Postives = 38/38 (100.00%), Query Frame = 0 

Query: 59 KMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          KMIDD++LGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 32 KMIDDRDLGFLANFLGIFIFALVIAYHYVTADPKYEGN 69

[Close]
BLAST of CmoCh15G004150 vs. NCBI nr
Match: KAA8518885.1 (hypothetical protein F0562_016341 [Nyssa sinensis])

HSP 1 Score: 80.5 bits (197), Expect = 8.8e-12
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 0 

Query: 55 NEYRKMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          N ++KMIDDQ+LGF ANFLGIFIFALVIAYHYV ADPKYEGN
Sbjct: 44 NRWQKMIDDQDLGFFANFLGIFIFALVIAYHYVVADPKYEGN 85

[Close]
BLAST of CmoCh15G004150 vs. NCBI nr
Match: XP_008463467.1 (PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucumis melo] >XP_008463468.1 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucumis melo] >XP_022939332.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucurbita moschata] >XP_022993977.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucurbita maxima] >XP_023551013.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucurbita pepo subsp. pepo] >XP_038890715.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Benincasa hispida] >XP_038890716.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Benincasa hispida] >XP_038890717.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Benincasa hispida])

HSP 1 Score: 79.7 bits (195), Expect = 1.5e-11
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]
BLAST of CmoCh15G004150 vs. NCBI nr
Match: XP_004142744.1 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucumis sativus])

HSP 1 Score: 79.3 bits (194), Expect = 2.0e-11
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          MIDDQELGFLANFLGIF+FALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFVFALVIAYHYVTADPKYEGN 37

[Close]
BLAST of CmoCh15G004150 vs. NCBI nr
Match: KCW49626.1 (hypothetical protein EUGRSUZ_K03148, partial [Eucalyptus grandis])

HSP 1 Score: 79.3 bits (194), Expect = 2.0e-11
Identity = 36/38 (94.74%), Postives = 38/38 (100.00%), Query Frame = 0 

Query: 59 KMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
          KMIDD++LGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 32 KMIDDRDLGFLANFLGIFIFALVIAYHYVTADPKYEGN 69

[Close]
BLAST of CmoCh15G004150 vs. NCBI nr
Match: OMO96944.1 (Oligosaccaryltransferase [Corchorus olitorius])

HSP 1 Score: 79.0 bits (193), Expect = 2.6e-11
Identity = 36/38 (94.74%), Postives = 37/38 (97.37%), Query Frame = 0 

Query: 59  KMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 97
           KMIDDQ+LGFLANFLGIFIFALVIAYHYVTADP YEGN
Sbjct: 69  KMIDDQQLGFLANFLGIFIFALVIAYHYVTADPNYEGN 106

[Close]
BLAST of CmoCh15G004150 vs. TAIR 10
Match: AT3G12587.1 (Oligosaccaryltransferase )

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-13
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 95
          MIDDQ+LGF+ANFLGIFIFALVIAYHYVTADPKYE
Sbjct: 1  MIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYE 35

[Close]
BLAST of CmoCh15G004150 vs. TAIR 10
Match: AT5G02502.1 (Oligosaccaryltransferase )

HSP 1 Score: 61.2 bits (147), Expect = 5.1e-10
Identity = 26/35 (74.29%), Postives = 31/35 (88.57%), Query Frame = 0 

Query: 60 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 95
          M DDQ+LGF ANFLGIFIF +VIAYH+V A+PK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFIMVIAYHFVVAEPKFE 35

The following BLAST results are available for this feature:
BLAST of CmoCh15G004150 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LHK32.4e-1294.29Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ara... [more]
C7J4U31.6e-1183.78Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ory... [more]
C7J0R53.8e-1078.38Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ory... [more]
Q9SF571.9e-0980.00Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Ara... [more]
Q8L9867.2e-0974.29Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ara... [more]
BLAST of CmoCh15G004150 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5J4ZJS34.2e-1285.71Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_016341 PE=3 SV=1[more]
A0A1S3CJC47.2e-12100.00dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cuc... [more]
A0A6J1JUF07.2e-12100.00dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cuc... [more]
A0A6J1FGH77.2e-12100.00dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cuc... [more]
A0A059A7I39.5e-1294.74Uncharacterized protein (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K03... [more]
BLAST of CmoCh15G004150 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA8518885.18.8e-1285.71hypothetical protein F0562_016341 [Nyssa sinensis][more]
XP_008463467.11.5e-11100.00PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subuni... [more]
XP_004142744.12.0e-1197.30dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucum... [more]
KCW49626.12.0e-1194.74hypothetical protein EUGRSUZ_K03148, partial [Eucalyptus grandis][more]
OMO96944.12.6e-1194.74Oligosaccaryltransferase [Corchorus olitorius][more]
BLAST of CmoCh15G004150 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G12587.11.7e-1394.29Oligosaccaryltransferase [more]
AT5G02502.15.1e-1074.29Oligosaccaryltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018943OligosaccaryltransferasePFAMPF10215Ost4coord: 60..92
e-value: 4.0E-15
score: 55.3
IPR044165Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4, plantPANTHERPTHR28677DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 4A-RELATEDcoord: 40..96
IPR036330Oligosaccharyltransferase subunit ost4p superfamilySUPERFAMILY103464Oligosaccharyltransferase subunit ost4pcoord: 60..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G004150.1CmoCh15G004150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016740 transferase activity