CmoCh15G002800 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G002800
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionE3 ubiquitin-protein ligase RNF181-like
LocationCmo_Chr15: 1337886 .. 1338572 (-)
RNA-Seq ExpressionCmoCh15G002800
SyntenyCmoCh15G002800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCGATTCCGACTCTTCTTCCTCTCCTCCGCCGTCGCTGCCCCCGCCTCGCAGCCTCTCTATGCTTCTCCCCTTTCTGCTTGGCCTTGCCCGAACCACCCCTGGATCGCACCTCAACCAGATCGTTGTTCTCGATCATGCTGCCCGAACGGTCGTCGTTTTTGAAGGTCGTGTTCTTGAGTCTTTTCTCCGCGACTTGTCGGAGAAATCCGGGCCGTTGCCTGCATCCAAGGCTTCGATCGATGCGATGCCGAGAGTCGGGATTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGACGGATTTGAAGTCGGTGCGGAGGCGAGAGAGATGCCTTGCAAACATCTGTATCATTCGGATTGTATTGAGAAATGGTTGAGGGTTCGTGGAACGTGTCCGATTTGTCGGTTCACGATGCCGGTGGACGATCAGGAAGGGAAGAAGGACGATGATGAAGATATGGAAGGAGACGAAGACCGAGTGGTTGTAGTGAATCTATGGGTTAACGACGGTGAACCAATGGATGTAGAATCGGTGGAATCGGGCTTGGGGCATGGATCGGGGGACTCCGATTCGGAATCTCACGATGAAATTACGGTCGAAGTTGACGCCTCATCAACGCAAGAACAAGATCCCAGTTCTGAAGATTCTTCGTCATTTTCATTTTCGTCCTTCTTCAACTAG

mRNA sequence

ATGTCCGATTCCGACTCTTCTTCCTCTCCTCCGCCGTCGCTGCCCCCGCCTCGCAGCCTCTCTATGCTTCTCCCCTTTCTGCTTGGCCTTGCCCGAACCACCCCTGGATCGCACCTCAACCAGATCGTTGTTCTCGATCATGCTGCCCGAACGGTCGTCGTTTTTGAAGGTCGTGTTCTTGAGTCTTTTCTCCGCGACTTGTCGGAGAAATCCGGGCCGTTGCCTGCATCCAAGGCTTCGATCGATGCGATGCCGAGAGTCGGGATTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGACGGATTTGAAGTCGGTGCGGAGGCGAGAGAGATGCCTTGCAAACATCTGTATCATTCGGATTGTATTGAGAAATGGTTGAGGGTTCGTGGAACGTGTCCGATTTGTCGGTTCACGATGCCGGTGGACGATCAGGAAGGGAAGAAGGACGATGATGAAGATATGGAAGGAGACGAAGACCGAGTGGTTGTAGTGAATCTATGGGTTAACGACGGTGAACCAATGGATGTAGAATCGGTGGAATCGGGCTTGGGGCATGGATCGGGGGACTCCGATTCGGAATCTCACGATGAAATTACGGTCGAAGTTGACGCCTCATCAACGCAAGAACAAGATCCCAGTTCTGAAGATTCTTCGTCATTTTCATTTTCGTCCTTCTTCAACTAG

Coding sequence (CDS)

ATGTCCGATTCCGACTCTTCTTCCTCTCCTCCGCCGTCGCTGCCCCCGCCTCGCAGCCTCTCTATGCTTCTCCCCTTTCTGCTTGGCCTTGCCCGAACCACCCCTGGATCGCACCTCAACCAGATCGTTGTTCTCGATCATGCTGCCCGAACGGTCGTCGTTTTTGAAGGTCGTGTTCTTGAGTCTTTTCTCCGCGACTTGTCGGAGAAATCCGGGCCGTTGCCTGCATCCAAGGCTTCGATCGATGCGATGCCGAGAGTCGGGATTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGACGGATTTGAAGTCGGTGCGGAGGCGAGAGAGATGCCTTGCAAACATCTGTATCATTCGGATTGTATTGAGAAATGGTTGAGGGTTCGTGGAACGTGTCCGATTTGTCGGTTCACGATGCCGGTGGACGATCAGGAAGGGAAGAAGGACGATGATGAAGATATGGAAGGAGACGAAGACCGAGTGGTTGTAGTGAATCTATGGGTTAACGACGGTGAACCAATGGATGTAGAATCGGTGGAATCGGGCTTGGGGCATGGATCGGGGGACTCCGATTCGGAATCTCACGATGAAATTACGGTCGAAGTTGACGCCTCATCAACGCAAGAACAAGATCCCAGTTCTGAAGATTCTTCGTCATTTTCATTTTCGTCCTTCTTCAACTAG

Protein sequence

MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVLESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Homology
BLAST of CmoCh15G002800 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 7.2e-23
Identity = 73/187 (39.04%), Postives = 101/187 (54.01%), Query Frame = 0

Query: 18  RSLSMLLPFLLGLARTTP--------GSHLNQIVVLDHAARTVVVFEGRV-LESFLRDL- 77
           R L + LPFLLG +             S   +I++++   + ++V EG   +   LR L 
Sbjct: 24  RDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRSLL 83

Query: 78  -SEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEKW 137
            S + G  PASKASIDAMP V I     +C ICL+ ++     +EMPCKH +H  CIEKW
Sbjct: 84  ESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIEKW 143

Query: 138 LRVRGTCPICRFTMPVD-DQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESVESGLG 193
           L   G+CP+CR+ MPVD D+ GKK +D    G+E   + V    NDG  +   S + G  
Sbjct: 144 LGFHGSCPVCRYEMPVDGDEIGKKRND----GNE---IWVRFSFNDGRRIRDFSAQDG-- 198

BLAST of CmoCh15G002800 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.1e-21
Identity = 45/101 (44.55%), Postives = 66/101 (65.35%), Query Frame = 0

Query: 55  FEGRVLESFLRDLSE----KSGPLPASKASIDAMPRVGITELGMD-----CAICLDGFEV 114
           F G  LE  ++ L+E    + G  PASK++I+A+P V IT+  ++     CA+C+D FE 
Sbjct: 174 FIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEE 233

Query: 115 GAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQE 147
           G EA++MPCKHLYH DC+  WL +  +CP+CR  +P DD +
Sbjct: 234 GTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPD 274

BLAST of CmoCh15G002800 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-19
Identity = 52/147 (35.37%), Postives = 76/147 (51.70%), Query Frame = 0

Query: 54  VFEGRVLESFLRDLSE---------KSGPLPASKASIDAMPRVGI----TELGMDCAICL 113
           +  G   E  L  LS+         +SG  PASK++I+++PRV I    T+   +CA+C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 114 DGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDED 173
           + FE G E REMPCKH++H DCI  WL +R +CP+CRF +P         D      +E+
Sbjct: 220 EVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP--------SDPIQRSNEEE 279

Query: 174 RVVVVNLWVNDGEPMDVESVESGLGHG 188
             V + +W   G    V    +G+  G
Sbjct: 280 HAVGMTIWRLPGGGFAVGRFNAGVREG 298

BLAST of CmoCh15G002800 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.8e-19
Identity = 46/123 (37.40%), Postives = 72/123 (58.54%), Query Frame = 0

Query: 55  FEGRVLESFLRDLSE----KSGPLPASKASIDAMPRVGITELGM-----DCAICLDGFEV 114
           F G  LE  ++ L+E    + G  PASK++IDA+P V +T+  +      CA+C+D FE 
Sbjct: 166 FFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED 225

Query: 115 GAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDD---------QEGKKDDDEDME 160
           G++ ++MPCKH++H DC+  WL +  +CP+CRF +P DD          +G  D    +E
Sbjct: 226 GSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVE 285

BLAST of CmoCh15G002800 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.3e-16
Identity = 63/194 (32.47%), Postives = 96/194 (49.48%), Query Frame = 0

Query: 54  VFEGRVLESFLRDLSE---------KSGPLPASKASIDAMPRVGITELGM----DCAICL 113
           +  G   E  L  LS+         +SG  PASK++I+++PRV I++  +    +CA+C 
Sbjct: 144 ILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCT 203

Query: 114 DGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDED------ 173
           + FE   EAREMPCKHL+H DCI  WL +R +CP+CRF +P +      +++ED      
Sbjct: 204 EIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNAVGMT 263

Query: 174 ---MEGDEDRVVVVNLWVNDGE---PMDVESVESGLGHGSGDSDSESHDEITVEVDASST 223
              + G    V   N  + DGE   P+ +  ++     G G  +S+        V +  T
Sbjct: 264 IWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMD-----GGGIGNSQDGPRRISWVRSHGT 323

BLAST of CmoCh15G002800 vs. ExPASy TrEMBL
Match: A0A6J1FLT0 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC111445129 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.1e-122
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

BLAST of CmoCh15G002800 vs. ExPASy TrEMBL
Match: A0A6J1JYB9 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489408 PE=4 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.7e-120
Identity = 225/228 (98.68%), Postives = 226/228 (99.12%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKH+YHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDE RVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

BLAST of CmoCh15G002800 vs. ExPASy TrEMBL
Match: A0A5A7T7P7 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00210 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 4.3e-95
Identity = 194/238 (81.51%), Postives = 203/238 (85.29%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELG DCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE-------GKKDDDEDMEGDED---RVVVVNLWVN 180
           DCIEKWL VRGTCP+CRF MPVDD+        G  ++DEDMEG E    RVVVVNLWVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVN 180

Query: 181 DGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           DGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 DGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 234

BLAST of CmoCh15G002800 vs. ExPASy TrEMBL
Match: A0A0A0KS87 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.8e-93
Identity = 194/244 (79.51%), Postives = 205/244 (84.02%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELGMDCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE--------GKKDDDEDM------EGDEDRVVVVN 180
           DCIEKWL VRGTCP+CRF MPVDD+         G ++++EDM      EG E RVVVVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFAMPVDDRRKDGGGGGGGDEEEEEDMEGGEGGEGGEGRVVVVN 180

Query: 181 LWVNDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASST--QEQDPSSEDSSSFSFS 229
           LWVNDGEPMD   VESGL HGS DSDSES DEI VEVD SST  QEQDPSSEDSSSFSFS
Sbjct: 181 LWVNDGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQEQDPSSEDSSSFSFS 240

BLAST of CmoCh15G002800 vs. ExPASy TrEMBL
Match: A0A1S3CC03 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.3e-88
Identity = 180/224 (80.36%), Postives = 189/224 (84.38%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELG DCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE-------GKKDDDEDMEGDED---RVVVVNLWVN 180
           DCIEKWL VRGTCP+CRF MPVDD+        G  ++DEDMEG E    RVVVVNLWVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVN 180

Query: 181 DGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPS 215
           DGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPS
Sbjct: 181 DGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS 220

BLAST of CmoCh15G002800 vs. NCBI nr
Match: XP_022939130.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata])

HSP 1 Score: 447.2 bits (1149), Expect = 8.5e-122
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

BLAST of CmoCh15G002800 vs. NCBI nr
Match: KAG6578580.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016134.1 E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 444.5 bits (1142), Expect = 5.5e-121
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDE RVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

BLAST of CmoCh15G002800 vs. NCBI nr
Match: XP_023551536.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 442.6 bits (1137), Expect = 2.1e-120
Identity = 226/228 (99.12%), Postives = 226/228 (99.12%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDE RVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

BLAST of CmoCh15G002800 vs. NCBI nr
Match: XP_022993365.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima])

HSP 1 Score: 441.8 bits (1135), Expect = 3.6e-120
Identity = 225/228 (98.68%), Postives = 226/228 (99.12%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKH+YHS
Sbjct: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESV 180
           DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDE RVVVVNLWVNDGEPMDVESV
Sbjct: 121 DCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEGRVVVVNLWVNDGEPMDVESV 180

Query: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 ESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

BLAST of CmoCh15G002800 vs. NCBI nr
Match: KAA0039492.1 (E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 357.5 bits (916), Expect = 8.8e-95
Identity = 194/238 (81.51%), Postives = 203/238 (85.29%), Query Frame = 0

Query: 1   MSDSDSSSSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEGRVL 60
           MSDSDSS SP P  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG VL
Sbjct: 1   MSDSDSSPSPSPP-PPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVL 60

Query: 61  ESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHS 120
           +S LRDLSEKSGPLPASKASIDAMPRVG+TELG DCAICLDGFEV  EAREMPCKH+YHS
Sbjct: 61  DSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHMYHS 120

Query: 121 DCIEKWLRVRGTCPICRFTMPVDDQE-------GKKDDDEDMEGDED---RVVVVNLWVN 180
           DCIEKWL VRGTCP+CRF MPVDD+        G  ++DEDMEG E    RVVVVNLWVN
Sbjct: 121 DCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNLWVN 180

Query: 181 DGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 229
           DGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 DGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 234

BLAST of CmoCh15G002800 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 5.1e-24
Identity = 73/187 (39.04%), Postives = 101/187 (54.01%), Query Frame = 0

Query: 18  RSLSMLLPFLLGLARTTP--------GSHLNQIVVLDHAARTVVVFEGRV-LESFLRDL- 77
           R L + LPFLLG +             S   +I++++   + ++V EG   +   LR L 
Sbjct: 24  RDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRSLL 83

Query: 78  -SEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEKW 137
            S + G  PASKASIDAMP V I     +C ICL+ ++     +EMPCKH +H  CIEKW
Sbjct: 84  ESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIEKW 143

Query: 138 LRVRGTCPICRFTMPVD-DQEGKKDDDEDMEGDEDRVVVVNLWVNDGEPMDVESVESGLG 193
           L   G+CP+CR+ MPVD D+ GKK +D    G+E   + V    NDG  +   S + G  
Sbjct: 144 LGFHGSCPVCRYEMPVDGDEIGKKRND----GNE---IWVRFSFNDGRRIRDFSAQDG-- 198

BLAST of CmoCh15G002800 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.7e-22
Identity = 45/120 (37.50%), Postives = 72/120 (60.00%), Query Frame = 0

Query: 66  DLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEK 125
           DL+ + G  PA+K +++A+  V I +  + C++CLD FE+G EA+EMPCKH +HSDC+  
Sbjct: 195 DLNNRYGTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLP 254

Query: 126 WLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNL-WVNDGEPMDVESVESGL 185
           WL +  +CP+CR+ +P  D +  K D E    D++   + N    ++G   D  S  S +
Sbjct: 255 WLELHSSCPVCRYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSSV 314

BLAST of CmoCh15G002800 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.7e-22
Identity = 45/120 (37.50%), Postives = 72/120 (60.00%), Query Frame = 0

Query: 66  DLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEK 125
           DL+ + G  PA+K +++A+  V I +  + C++CLD FE+G EA+EMPCKH +HSDC+  
Sbjct: 195 DLNNRYGTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLP 254

Query: 126 WLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNL-WVNDGEPMDVESVESGL 185
           WL +  +CP+CR+ +P  D +  K D E    D++   + N    ++G   D  S  S +
Sbjct: 255 WLELHSSCPVCRYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSSV 314

BLAST of CmoCh15G002800 vs. TAIR 10
Match: AT3G13430.3 (RING/U-box superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.7e-22
Identity = 45/120 (37.50%), Postives = 72/120 (60.00%), Query Frame = 0

Query: 66  DLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHLYHSDCIEK 125
           DL+ + G  PA+K +++A+  V I +  + C++CLD FE+G EA+EMPCKH +HSDC+  
Sbjct: 195 DLNNRYGTPPATKEAVEALAMVKIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLP 254

Query: 126 WLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDEDRVVVVNL-WVNDGEPMDVESVESGL 185
           WL +  +CP+CR+ +P  D +  K D E    D++   + N    ++G   D  S  S +
Sbjct: 255 WLELHSSCPVCRYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSSV 314

BLAST of CmoCh15G002800 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 98.2 bits (243), Expect = 9.0e-21
Identity = 52/147 (35.37%), Postives = 76/147 (51.70%), Query Frame = 0

Query: 54  VFEGRVLESFLRDLSE---------KSGPLPASKASIDAMPRVGI----TELGMDCAICL 113
           +  G   E  L  LS+         +SG  PASK++I+++PRV I    T+   +CA+C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 114 DGFEVGAEAREMPCKHLYHSDCIEKWLRVRGTCPICRFTMPVDDQEGKKDDDEDMEGDED 173
           + FE G E REMPCKH++H DCI  WL +R +CP+CRF +P         D      +E+
Sbjct: 220 EVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP--------SDPIQRSNEEE 279

Query: 174 RVVVVNLWVNDGEPMDVESVESGLGHG 188
             V + +W   G    V    +G+  G
Sbjct: 280 HAVGMTIWRLPGGGFAVGRFNAGVREG 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQX27.2e-2339.04E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ... [more]
P0CH305.1e-2144.55E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q9SNB61.3e-1935.37E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ... [more]
Q8LPN74.8e-1937.40E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q940T51.3e-1632.47E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FLT04.1e-122100.00E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JYB91.7e-12098.68E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489... [more]
A0A5A7T7P74.3e-9581.51E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0KS871.8e-9379.51RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 P... [more]
A0A1S3CC031.3e-8880.36E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 P... [more]
Match NameE-valueIdentityDescription
XP_022939130.18.5e-122100.00E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata][more]
KAG6578580.15.5e-12199.56E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023551536.12.1e-12099.12E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo][more]
XP_022993365.13.6e-12098.68E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima][more]
KAA0039492.18.8e-9581.51E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3... [more]
Match NameE-valueIdentityDescription
AT1G26800.15.1e-2439.04RING/U-box superfamily protein [more]
AT3G13430.13.7e-2237.50RING/U-box superfamily protein [more]
AT3G13430.23.7e-2237.50RING/U-box superfamily protein [more]
AT3G13430.33.7e-2237.50RING/U-box superfamily protein [more]
AT3G46620.19.0e-2135.37zinc finger (C3HC4-type RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 96..136
e-value: 1.2E-8
score: 44.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 95..137
e-value: 3.3E-14
score: 53.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 96..137
score: 12.415513
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 95..137
e-value: 0.0021
score: 13.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 63..143
e-value: 9.6E-21
score: 75.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 170..228
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 42..152
NoneNo IPR availablePANTHERPTHR15710:SF132E3 UBIQUITIN-PROTEIN LIGASE MPSR1coord: 42..152
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 95..137
e-value: 6.22691E-21
score: 80.4237
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 95..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G002800.1CmoCh15G002800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding