CmoCh15G001900 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G001900
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCmo_Chr15: 871538 .. 876045 (+)
RNA-Seq ExpressionCmoCh15G001900
SyntenyCmoCh15G001900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATATATATATACCCGCACATACATTTGTAATCATGATTTCCAATTCGTAAATATTTGAAGAGCGCCATGGGAGAGAAAGAAAACCTGGCGCCAAGTGCAGTGGGCTCTGTTGCAGTGCAGAAACCGCCGCACTTCGTGCTCGTTCATGGCATAAGTGGAGGAGGATGGTGCTGGTACAAAATCAGGTGCTTAATGGAGAATTCTGGCTTCAAAGTCACCTGTATTGATCTTAAAGGCGCTGGAATTGATTTATCCGATCCGAATTCCGTCGTCAAATTCGACGATTACAATAAGCCTCTGCTCGATTTCGTCTCCAGTCTACCGGAAAACGAACAGGTAATATCTATTCGTCGTTTCTAATTTATTTCTTTGCCGGAAAGTCTCTGTTCCTCAATCGACTACGATCGAGTGGAGTTGATTGATTCAGAGATCGTCGAAAATTTTTACAGATCATTCTGGTTGGACATAGCGCCGGAGGATTAAGTGTTACTCAAGCAACGCTCAAATTCGCAAAGAAAATCCGTTTGGCGGTGTATGTGGCGGCGACGATGCTGAGATTTGGTTTCCAGACTGATCAAGATATCAAAAACGTAAACAAATTCTTCTTCTCACTACTTTTAATTCATATAAACTCATCCTTAATTTGTGTGTGTGTTTACAAATCCTAATCGAAGTAAAACTAGGCGTATTAGTGCAGCGTTCCTTTTTACGAGTTGGCCGGCCGATCGGCGAGGTTCTTGTGAAACTCGTCGCCGGCCGGTGGTGGTCATATGATAGTGAATCGTGTGAATAGTTTTGGCCATTAGTGGAATTGAAGGCTCTTGCTCTCATCGCAAATCGCATGAGGCAGGAAAAACACCGGCTGCTAAGTGCCAATTTATTCAAAGTCATCAATCAATTAAGTCTAACCGTTTTGTCTTCTAATTAGGAAAATGAATTAAGGACAACCACCAGCCAGCTGGTTGCTGAATTGAACATTTCATTTCGCCTCTAACTGTAGGACTATTGGCATCCTCTCACTCTGACTCATAATAGTCTGAGTAACGATTCCATTATATTGAATAATTCTGGTAGCAAAATTTATTTCAGGTGAGTTTTCATTGTGTCGGTTACTTCATTTTATTTATTGGAGGTTACATGAATTTGGAATTTTTTCTTAAAAAGAAAAAAATACACCACGTCAGTAGAAACTCTTTTTCAACCTTTTAAATACGAAAATGATGCATTAATAAAGAAGCTACGTTTAAGTTAATCCATAATTTAGGTCTAGTTTCCTTTAGATTATTACAATTCATTTAAAAATTAGCATTTATAGTTTCTATATAGTCAGTGATTAGTAATTCATAAGCAAAACGATAGTACCTAAATTGTGAGTTGAAAGGGAAAAAGGAAAAAAATTACATAATTGATAATTTGAAGTTGAGCTTGCTTGGATGTGATTTTTATCCTGAAGTAAATCGGTACGGTGGAACCGCAGAACCGCATAGGCAGCCAAGAACATGTCCCCCTTTGTGTGGTGATTTTTTTTTTGGTGGTGTAAATTTTCTGTGCTACAGCCACAGGAAAATACAAACTCTAGTTTAGAGTTAAATTCTGATTTTTAAATTTAATACTTCCGAGTTTAGAATTAAAATTCAATTGAGTTTCTTGGCTTAAAAATCTGACCATTTAGTTTTCATCTTTTGAAAAATAGTTTCATATATTTCTGAAAATTAAATCTTTGTTCGTGTCTAGGTTGTGTTTTTTTAGATGAGGTGGCAAGGTAGGATTGTAAAAATTATAAAATTGTCTGCTAATAATTTTAATAAGGTTGAATATTATAAAGAATTAATGAAAATCTTTTTGGCCGTAAAAAAAAAAAAAAAAGTCAATGTACTCTGTTTAAAGAAAGAATTAAGTAAGTGTAGTTAATTATAGCTTATAGGTAGATTTAATTTTTAATTATAGCTTATAGGTAGATTTAATTTTTAGTTATAGGTTATATCAATGTAATCCAAGATTACAAACATTCCATTAGAAGAATATTAAGGCTATGCATATAAAAATAAGGATGTAATTAAAGTATGTTCTGTATGCTGCAACAGTGTTTGGAATATTTTCCGTGGTTTTGTACGAAAGAAAGTCAAAATTAGGTTAAGGCCAGTTGGAGTTCTCAAAGTCCGGAAGGTAATAGTGTGCTGAAGACTCAAGAGGAGCCGACAGGTTTCACAAGTTCACAGTTCTCACGTGTGCACTCAAAATTTTCATTTATTAGCCTAATTTCTTTAATGATCAAGGAAAATACAATGCAATGCCATTCTATTTCCCCTTCTGATTCTGATTGGACATAGGACACAGGAATTCCTGCTGCTGCTGCCCATTTACCCCAATCCAAAATTGACATGTCTTATTTATTCAAGTTCTTTATTTTTTCTATATTCAAAGAAATAGTGCCCCCACCCTTGATTCCTTTCCCTGTCAATGTACCTCTCTCCCTACTCATTGAGCTTAACCTCTCTAGTCCTTCCCTCTCTCTCTCTCTAGTCTCGTCCCACACCTATCTCTTTCCTTTCATTTGCTACCAACCCCCTTGAAATAGTTTTGGTACCGAAAACACTCCCAAGGCATAGCAAAACCCTTGGAGGAGGCCTAATGAAGAAGGAAGGAGCGAAACAATAGAAGATCTTTTTTGGCTAAAAATTGCAATATATATACACGGGATTTCCCGATATATACGATTGATTGAACTTGAGCTGTTTAGGGGAGAGTGAGAAGGAAGGATTGGAGGCTACCCTCTAATGAAAAGGAAGGGGATAGGGTGGGATACTATTTTTTTGGATAAAAACCGCTGCAATTCTAGATTGGGCCACGCGCCCTAAGCAGTAGGGGAGTAAAATTTTCCCGTGTCCTACATAATATGTTGGGCCGGTCTCTACTTACTTTTTCTTATATTATATTATATAATATAAGATTAAGATGATTAGATCATTATTAACGAGTTAACCATTTCCGTTATGATTATTACCTTTTGTTTTTGTTTTGGTCAAATGTGCAGGGAGTGCCAGATTTATCCGAGTTTGGGGATGCTTACGACGTAGGATTTGGACAAGGACCCCAACAACCTCCCACCACTGCTGTCGTCAAAAGGGAACTCCAGCGAAAGATCATCTATCACATGAGTCCCCACGAGGTACCTAAACCCACAAAAATAAAAATAATAATAAACATAGTTTCTTAGTTTTGGAATCATCCCCAAATCCAACCACGAGATTCTTTCCTGCCTTGAGATTTTTAATGTAATTGCATTTGATCCTAAAAACATGCCTGAAAGTGTTCTAAAACGTTTAAAATCATTTGACCAGTCAAAATCTATTTAATATTTGATCTTACGCTGCCAAAATTGATTTGATTTTGAACATGCCATCCCAAACAAACATGCCATGAATTAAGAAGCAATTGTATCTAACCTCCCATCATGATACCCCGTGTTTTGATGTTATCAATGATTCTCAGGATTCGACACTGGCCTCCATGCTATTGAGGCCAGGGCCAATCCTCGCATTAAGGACCGCTCGGTTTAGGGAGGAGAACGGCGAGCTGGATAGGGTGAAACGAGTGTATATAAGAACAACGTACGATCGAGTAATAAAACCAGAGCAACAAGAGGCGATGATAAGGGAATGGCTGCCCGAAATCGTATATGAAATGGATACTGATCACAGCCCCTTTTTCTCCAACCCCTCTCTGCTCTTCGGCTTGCTCGTAAAAGCTGCTGCTGCTGCCGCCGCCTCCTCAACGGCGGCATGTAACTAAACAAGGGGAGGAAGAGGGCATTTTTCATTTTCACTCTCTTTCTCTCTCTTATGCTAAAATAAGAACATCTGAAAATTCTTGTGATTCAACAATAAAACTACAATTATATAGTGCTTCGTAATTGTTTCAAATTATGCATTTGACAGCAGTACATCGTGTTCTCTTGCAAGCAAGGAACCATAAAGAATGATGACTTATCCTCTGAGCATGGAACGAATTTCTCACTGAAATTGGGTAAGAAAGCACAAAAGAATTCCTCCCTCTAATGCACTTTTTTTTGTTTTTTTCTTTTTGTTTTCCTTGTTGTTTTGTTATGGGAATTTGTATTGTGCTTTCGGTGTTCTATTATGTATCAGTGAATGGGGAAGAAAAAGTATCAAGCCTCGTCCATGGCAGCTATCTTCTGTTGCAGATCTGACTTGTCAGCTCCTATTAATTTATCTACTTGGCGACCATCTTTAAAGAACACGAATGTTGGAGTGGCTTTGATGTCCCACGATTTACTGAGCTCCTGCAAAATAAATAAAATTAAAATTAAAAAAAAAACCCACCTCACCCGACCTCAGTCTAAGAAACTTCTAAAACTCACCGTACTATACAATAAACTATACCATTATCAATTTAAATTAGGATTTAGGACACTTTTTTTTTGTAATGTTTTCAATTCTTTGTTTCTCTTTTTAACTCAATAAATTAAAATATATGTCGTCCACCA

mRNA sequence

TATATATATATACCCGCACATACATTTGTAATCATGATTTCCAATTCGTAAATATTTGAAGAGCGCCATGGGAGAGAAAGAAAACCTGGCGCCAAGTGCAGTGGGCTCTGTTGCAGTGCAGAAACCGCCGCACTTCGTGCTCGTTCATGGCATAAGTGGAGGAGGATGGTGCTGGTACAAAATCAGGTGCTTAATGGAGAATTCTGGCTTCAAAGTCACCTGTATTGATCTTAAAGGCGCTGGAATTGATTTATCCGATCCGAATTCCGTCGTCAAATTCGACGATTACAATAAGCCTCTGCTCGATTTCGTCTCCAGTCTACCGGAAAACGAACAGATCATTCTGGTTGGACATAGCGCCGGAGGATTAAGTGTTACTCAAGCAACGCTCAAATTCGCAAAGAAAATCCGTTTGGCGGTGTATGTGGCGGCGACGATGCTGAGATTTGGTTTCCAGACTGATCAAGATATCAAAAACGGAGTGCCAGATTTATCCGAGTTTGGGGATGCTTACGACGTAGGATTTGGACAAGGACCCCAACAACCTCCCACCACTGCTGTCGTCAAAAGGGAACTCCAGCGAAAGATCATCTATCACATGAGTCCCCACGAGTTTCTTAGTTTTGGAATCATCCCCAAATCCAACCACGAGATTCTTTCCTGCCTTGAGATTTTTAATGATTCGACACTGGCCTCCATGCTATTGAGGCCAGGGCCAATCCTCGCATTAAGGACCGCTCGGTTTAGGGAGGAGAACGGCGAGCTGGATAGGGTGAAACGAGTGTATATAAGAACAACGTACGATCGAGTAATAAAACCAGAGCAACAAGAGGCGATGATAAGGGAATGGCTGCCCGAAATCGTATATGAAATGGATACTGATCACAGCCCCTTTTTCTCCAACCCCTCTCTGCTCTTCGGCTTGCTCGTAAAAGCTGCTGCTGCTGCCGCCGCCTCCTCAACGGCGGCATGTAACTAAACAAGGGGAGGAAGAGGGCATTTTTCATTTTCACTCTCTTTCTCTCTCTTATGCTAAAATAAGAACATCTGAAAATTCTTGTGATTCAACAATAAAACTACAATTATATAGTGCTTCGTAATTGTTTCAAATTATGCATTTGACAGCAGTACATCGTGTTCTCTTGCAAGCAAGGAACCATAAAGAATGATGACTTATCCTCTGAGCATGGAACGAATTTCTCACTGAAATTGGTGAATGGGGAAGAAAAAGTATCAAGCCTCGTCCATGGCAGCTATCTTCTGTTGCAGATCTGACTTGTCAGCTCCTATTAATTTATCTACTTGGCGACCATCTTTAAAGAACACGAATGTTGGAGTGGCTTTGATGTCCCACGATTTACTGAGCTCCTGCAAAATAAATAAAATTAAAATTAAAAAAAAAACCCACCTCACCCGACCTCAGTCTAAGAAACTTCTAAAACTCACCGTACTATACAATAAACTATACCATTATCAATTTAAATTAGGATTTAGGACACTTTTTTTTTGTAATGTTTTCAATTCTTTGTTTCTCTTTTTAACTCAATAAATTAAAATATATGTCGTCCACCA

Coding sequence (CDS)

ATGGGAGAGAAAGAAAACCTGGCGCCAAGTGCAGTGGGCTCTGTTGCAGTGCAGAAACCGCCGCACTTCGTGCTCGTTCATGGCATAAGTGGAGGAGGATGGTGCTGGTACAAAATCAGGTGCTTAATGGAGAATTCTGGCTTCAAAGTCACCTGTATTGATCTTAAAGGCGCTGGAATTGATTTATCCGATCCGAATTCCGTCGTCAAATTCGACGATTACAATAAGCCTCTGCTCGATTTCGTCTCCAGTCTACCGGAAAACGAACAGATCATTCTGGTTGGACATAGCGCCGGAGGATTAAGTGTTACTCAAGCAACGCTCAAATTCGCAAAGAAAATCCGTTTGGCGGTGTATGTGGCGGCGACGATGCTGAGATTTGGTTTCCAGACTGATCAAGATATCAAAAACGGAGTGCCAGATTTATCCGAGTTTGGGGATGCTTACGACGTAGGATTTGGACAAGGACCCCAACAACCTCCCACCACTGCTGTCGTCAAAAGGGAACTCCAGCGAAAGATCATCTATCACATGAGTCCCCACGAGTTTCTTAGTTTTGGAATCATCCCCAAATCCAACCACGAGATTCTTTCCTGCCTTGAGATTTTTAATGATTCGACACTGGCCTCCATGCTATTGAGGCCAGGGCCAATCCTCGCATTAAGGACCGCTCGGTTTAGGGAGGAGAACGGCGAGCTGGATAGGGTGAAACGAGTGTATATAAGAACAACGTACGATCGAGTAATAAAACCAGAGCAACAAGAGGCGATGATAAGGGAATGGCTGCCCGAAATCGTATATGAAATGGATACTGATCACAGCCCCTTTTTCTCCAACCCCTCTCTGCTCTTCGGCTTGCTCGTAAAAGCTGCTGCTGCTGCCGCCGCCTCCTCAACGGCGGCATGTAACTAA

Protein sequence

MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTAACN
Homology
BLAST of CmoCh15G001900 vs. ExPASy Swiss-Prot
Match: Q9SG92 (Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.3e-92
Identity = 161/282 (57.09%), Postives = 211/282 (74.82%), Query Frame = 0

Query: 18  QKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKP 77
           +KPPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YN+P
Sbjct: 16  RKPPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQP 75

Query: 78  LLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKN 137
           L+DF+SS PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G QTD+D+K+
Sbjct: 76  LIDFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKD 135

Query: 138 GVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEIL 197
           GVPDLSE GD Y++GFG GP+ PPT+A++K E +RK++YHMSP +               
Sbjct: 136 GVPDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQ--------------- 195

Query: 198 SCLEIFNDSTLASMLLRPGPILALRTARFRE---ENGELDRVKRVYIRTTYDRVIKPEQQ 257
                  + +LA++++RP PILAL TA+  E   E G+ ++V RVYI+T  DRV+KPEQQ
Sbjct: 196 -------ECSLAALMMRPAPILALTTAKLEEEEKEKGQEEQVPRVYIKTLLDRVMKPEQQ 255

Query: 258 EAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAA 297
           +AMIR W P  VYE+++DHSPFFSNP +LFGLL+KAA +  +
Sbjct: 256 DAMIRRWPPSQVYELESDHSPFFSNPFVLFGLLIKAAVSVGS 275

BLAST of CmoCh15G001900 vs. ExPASy Swiss-Prot
Match: Q2V0W1 (Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.8e-52
Identity = 110/272 (40.44%), Postives = 158/272 (58.09%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG S G WCWYK+  L+  +GFK T +DL GAGI+L+D N+V  FD YN+PL   
Sbjct: 13  HFVFVHGASHGAWCWYKLTTLLVAAGFKATSVDLTGAGINLTDSNTVFDFDHYNRPLFSL 72

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNGVPD 141
           +S LP + +I+LVGHS GG SVT+A  KF  KI + VY+AA M++ G  +     + +  
Sbjct: 73  LSDLPSHHKIVLVGHSIGGGSVTEALCKFTDKISMVVYLAADMVQPG--STSSTHDSIMT 132

Query: 142 LSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEILSCLE 201
           + E  D ++  +G+G  +PPT  ++K E +R   Y  SP E                   
Sbjct: 133 VGE-EDIWEYIYGEGADKPPTGVLMKEEFRRHYYYSQSPLE------------------- 192

Query: 202 IFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREW 261
              D +LAS LLRP P+ AL  A     N E ++V RVYI+T  D +  P +Q+ ++ +W
Sbjct: 193 ---DVSLASKLLRPAPVRALGGADKLSPNPEAEKVPRVYIKTAKDNLFDPLRQDRLVEKW 252

Query: 262 LPEIVYEM-DTDHSPFFSNPSLLFGLLVKAAA 293
            P  +Y + ++DHS FFS P+ LF  L++A +
Sbjct: 253 PPSQLYILEESDHSAFFSVPTTLFAYLLRAVS 259

BLAST of CmoCh15G001900 vs. ExPASy Swiss-Prot
Match: Q9LVL9 (Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.9e-51
Identity = 113/278 (40.65%), Postives = 157/278 (56.47%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V   DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNGVPD 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G    + +KN +  
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 142 LS-----EFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEI 201
            S     E    +D  FG GPQ  PT+ ++K E  R   Y+ SP E              
Sbjct: 125 CSGLIEEETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPME-------------- 184

Query: 202 LSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEA 261
                   D TLA+ LLRP P++A           E D++ RVY++T  D + +P  QE 
Sbjct: 185 --------DYTLATTLLRPAPVMAFIGIMDIPGAPETDKIPRVYVKTGKDHLFEPVLQEV 244

Query: 262 MIREWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAAAA 294
           M+  W P   + + D+DHS FFS P  L+  L++AA++
Sbjct: 245 MLALWPPAHTFLLPDSDHSAFFSQPQELYQFLLQAASS 260

BLAST of CmoCh15G001900 vs. ExPASy Swiss-Prot
Match: O23512 (Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.5e-49
Identity = 109/275 (39.64%), Postives = 152/275 (55.27%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI L D N V   D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKN---G 141
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G      + N   G
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSNIHVG 131

Query: 142 VPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEILS 201
             D+ E+       +G+G  +PPT  ++K E  R   Y  SP E                
Sbjct: 132 EEDIWEY------TYGEGTDKPPTGVLMKPEFIRHYYYSQSPLE---------------- 191

Query: 202 CLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMI 261
                 D TL+S LLRP P+ A +       N E ++V RVYI+T  D +    +Q+ ++
Sbjct: 192 ------DVTLSSKLLRPAPMRAFQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLV 251

Query: 262 REWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAAA 293
             W P  +Y + D+DHS FFS P+ LF  L++A +
Sbjct: 252 ENWPPSQLYVLEDSDHSAFFSVPTTLFAYLLRAVS 258

BLAST of CmoCh15G001900 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.0e-38
Identity = 93/287 (32.40%), Postives = 154/287 (53.66%), Query Frame = 0

Query: 9   PSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSV 68
           P    S+ V+K   FVLVHG   G WCWYK+   +E SG     +DL G G +++D N+V
Sbjct: 87  PDFSESLIVKK---FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTV 146

Query: 69  VKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG 128
              ++Y+KPL+D + +LPE E++ILVGHS GG S++ A  +F +KI  A++V ATM+  G
Sbjct: 147 STLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDG 206

Query: 129 FQTDQDIKNGVPDLSEF-GDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFG 188
            +        +     F  ++  + +G G  +PPT  + ++   + + ++ SP++     
Sbjct: 207 QRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNK----- 266

Query: 189 IIPKSNHEILSCLEIFNDSTLASMLLRPGPI--LALRTARFREENGELDRVKRVYIRTTY 248
                            D  LA + +RP P+  +  + +   E  G   + +R Y++T  
Sbjct: 267 -----------------DIALAMISMRPVPLGPMMEKVSLTAERYG---KGRRFYVQTLD 326

Query: 249 DRVIKPEQQEAMIREWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAA 292
           DR + P+ QE ++RE  PE V+++  +DH PFFS P  L  +L++ A
Sbjct: 327 DRALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIA 345

BLAST of CmoCh15G001900 vs. ExPASy TrEMBL
Match: A0A6J1FL47 (methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.1e-154
Identity = 281/303 (92.74%), Postives = 281/303 (92.74%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240
           HE                      DSTLASMLLRPGPILALRTARFREENGELDRVKRVY
Sbjct: 181 HE----------------------DSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240

Query: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA 300
           IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA
Sbjct: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA 281

Query: 301 ACN 304
           ACN
Sbjct: 301 ACN 281

BLAST of CmoCh15G001900 vs. ExPASy TrEMBL
Match: A0A6J1JV68 (methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.8e-150
Identity = 276/303 (91.09%), Postives = 277/303 (91.42%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTDQDIKNGVPDLSEFGD YDVGFG GPQQPPTTAV+KRELQRKIIYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240
           HE                      DSTLASMLLRPGPILALRTARFREENGELD VKRVY
Sbjct: 181 HE----------------------DSTLASMLLRPGPILALRTARFREENGELDTVKRVY 240

Query: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA 300
           IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK AAAAAASSTA
Sbjct: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK-AAAAAASSTA 280

Query: 301 ACN 304
           ACN
Sbjct: 301 ACN 280

BLAST of CmoCh15G001900 vs. ExPASy TrEMBL
Match: A0A5A7TBF5 (Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001670 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.1e-128
Identity = 229/294 (77.89%), Postives = 251/294 (85.37%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV++  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSV+ FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTDQDIK+GVPDLSE+GD Y++ FG GPQQPPTTAV+KRE QRK+ YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENG--ELDRVKR 240
           HE                      DSTLA+MLLRPGPI AL +A+F+EE    E D+VKR
Sbjct: 181 HE----------------------DSTLAAMLLRPGPIQALMSAQFKEEEDREEADKVKR 240

Query: 241 VYIRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAA 293
           VYIRT YDRVIKPEQQEAMI+ W PEIVYEMDTDHSPFFSNPSLLFGLL+K+ +
Sbjct: 241 VYIRTMYDRVIKPEQQEAMIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCS 272

BLAST of CmoCh15G001900 vs. ExPASy TrEMBL
Match: A0A1S3CAT2 (methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.1e-128
Identity = 229/294 (77.89%), Postives = 251/294 (85.37%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV++  PPHFVLVHGI GGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVNSVSLHNPPHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSV+ FDDYN+PLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTDQDIK+GVPDLSE+GD Y++ FG GPQQPPTTAV+KRE QRK+ YHMSP
Sbjct: 121 AATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENG--ELDRVKR 240
           HE                      DSTLA+MLLRPGPI AL +A+F+EE    E D+VKR
Sbjct: 181 HE----------------------DSTLAAMLLRPGPIQALMSAQFKEEEDREEADKVKR 240

Query: 241 VYIRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAA 293
           VYIRT YDRVIKPEQQEAMI+ W PEIVYEMDTDHSPFFSNPSLLFGLL+K+ +
Sbjct: 241 VYIRTMYDRVIKPEQQEAMIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCS 272

BLAST of CmoCh15G001900 vs. ExPASy TrEMBL
Match: A0A6J1BYB8 (methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.8e-126
Identity = 232/303 (76.57%), Postives = 255/303 (84.16%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60
           MGE++NLAPSAV SVAVQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEEDNLAPSAV-SVAVQKPLPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSV+KFDDYNKPLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIY 180
           AVYVAATML+ G+QTDQDIK+GVPDLSEFGD YD+ FG G QQPPTTA++KRE QRKI Y
Sbjct: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180

Query: 181 HMSPHEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRV 240
           HMSPHE                      DSTL +MLLRPGPILAL+TA+FREE    D+V
Sbjct: 181 HMSPHE----------------------DSTLGAMLLRPGPILALKTAQFREEEEFADKV 240

Query: 241 KRVYIRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAA 300
           KRV+IRT YDRVIK EQQ+AMI+ W PEIVY+MDTDHSPFFSNPSLLFGLL+KAAA+   
Sbjct: 241 KRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC 280

BLAST of CmoCh15G001900 vs. NCBI nr
Match: XP_022938905.1 (methylesterase 17 [Cucurbita moschata] >XP_023551105.1 methylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 553.9 bits (1426), Expect = 8.5e-154
Identity = 281/303 (92.74%), Postives = 281/303 (92.74%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240
           HE                      DSTLASMLLRPGPILALRTARFREENGELDRVKRVY
Sbjct: 181 HE----------------------DSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240

Query: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA 300
           IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA
Sbjct: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA 281

Query: 301 ACN 304
           ACN
Sbjct: 301 ACN 281

BLAST of CmoCh15G001900 vs. NCBI nr
Match: KAG6578491.1 (Methylesterase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 549.3 bits (1414), Expect = 2.1e-152
Identity = 281/304 (92.43%), Postives = 281/304 (92.43%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240
           HE                      DSTLASMLLRPGPILALRTARFREENGELDRVKRVY
Sbjct: 181 HE----------------------DSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240

Query: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK-AAAAAAASST 300
           IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK AAAAAAASST
Sbjct: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAAASST 282

Query: 301 AACN 304
           AACN
Sbjct: 301 AACN 282

BLAST of CmoCh15G001900 vs. NCBI nr
Match: XP_022993026.1 (methylesterase 17 [Cucurbita maxima])

HSP 1 Score: 541.2 bits (1393), Expect = 5.7e-150
Identity = 276/303 (91.09%), Postives = 277/303 (91.42%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTDQDIKNGVPDLSEFGD YDVGFG GPQQPPTTAV+KRELQRKIIYHMSP
Sbjct: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240
           HE                      DSTLASMLLRPGPILALRTARFREENGELD VKRVY
Sbjct: 181 HE----------------------DSTLASMLLRPGPILALRTARFREENGELDTVKRVY 240

Query: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAASSTA 300
           IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK AAAAAASSTA
Sbjct: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVK-AAAAAASSTA 280

Query: 301 ACN 304
           ACN
Sbjct: 301 ACN 280

BLAST of CmoCh15G001900 vs. NCBI nr
Match: KAG7016056.1 (Methylesterase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 515.8 bits (1327), Expect = 2.6e-142
Identity = 281/380 (73.95%), Postives = 281/380 (73.95%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV
Sbjct: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKN------------------------------------------- 180
           AATMLRFGFQTDQDIKN                                           
Sbjct: 121 AATMLRFGFQTDQDIKNVNKFFFSLLLIHINSSLICDYWHPLTLTHNSLSNDSIMLNNSG 180

Query: 181 ---------------------------------GVPDLSEFGDAYDVGFGQGPQQPPTTA 240
                                            GVPDLSEFGDAYDVGFGQGPQQPPTTA
Sbjct: 181 SKIYFSVWNIFRGFVRKKVKIRLRPVGVLKVRKGVPDLSEFGDAYDVGFGQGPQQPPTTA 240

Query: 241 VVKRELQRKIIYHMSPHEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTA 300
           VVKRELQRKIIYHMSPHE                      DSTLASMLLRPGPILALRTA
Sbjct: 241 VVKRELQRKIIYHMSPHE----------------------DSTLASMLLRPGPILALRTA 300

Query: 301 RFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLF 304
           RFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLF
Sbjct: 301 RFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLF 358

BLAST of CmoCh15G001900 vs. NCBI nr
Match: XP_038890470.1 (methylesterase 17 [Benincasa hispida])

HSP 1 Score: 481.1 bits (1237), Expect = 7.0e-132
Identity = 237/292 (81.16%), Postives = 254/292 (86.99%), Query Frame = 0

Query: 1   MGEKENLAPSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60
           MGEKENLAPS+V SV+VQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI
Sbjct: 1   MGEKENLAPSSVDSVSVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGI 60

Query: 61  DLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYV 120
           D SDPNSV+KFDDYNKPLLDF+SSLPENEQIILVGHSAGGLS+TQATLKFAKKIRLAVYV
Sbjct: 61  DRSDPNSVLKFDDYNKPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRLAVYV 120

Query: 121 AATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSP 180
           AATMLRFGFQTD+D K+GVPDLSEFGD YDV FG GPQQPPTTAV+KRE QRKIIYHMSP
Sbjct: 121 AATMLRFGFQTDRDFKDGVPDLSEFGDVYDVEFGLGPQQPPTTAVIKREFQRKIIYHMSP 180

Query: 181 HEFLSFGIIPKSNHEILSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVY 240
           HE                      DSTLA+MLLRPGPILAL+TA+F EE    D+VKRVY
Sbjct: 181 HE----------------------DSTLAAMLLRPGPILALKTAQFMEEQDGADKVKRVY 240

Query: 241 IRTTYDRVIKPEQQEAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAA 293
           IRT YDRVIKPEQQ+AMI+ W PEIVYEMDTDHSPFFS+PSLLFGLLVK+ +
Sbjct: 241 IRTMYDRVIKPEQQDAMIKGWPPEIVYEMDTDHSPFFSDPSLLFGLLVKSCS 270

BLAST of CmoCh15G001900 vs. TAIR 10
Match: AT3G10870.1 (methyl esterase 17 )

HSP 1 Score: 339.7 bits (870), Expect = 2.4e-93
Identity = 161/282 (57.09%), Postives = 211/282 (74.82%), Query Frame = 0

Query: 18  QKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKP 77
           +KPPHFVL+HG+S G WCWYKI+CLME SGF VTCIDLK +GID S  +S+  FD YN+P
Sbjct: 16  RKPPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQYNQP 75

Query: 78  LLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKN 137
           L+DF+SS PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+ML+ G QTD+D+K+
Sbjct: 76  LIDFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDEDMKD 135

Query: 138 GVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEIL 197
           GVPDLSE GD Y++GFG GP+ PPT+A++K E +RK++YHMSP +               
Sbjct: 136 GVPDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQ--------------- 195

Query: 198 SCLEIFNDSTLASMLLRPGPILALRTARFRE---ENGELDRVKRVYIRTTYDRVIKPEQQ 257
                  + +LA++++RP PILAL TA+  E   E G+ ++V RVYI+T  DRV+KPEQQ
Sbjct: 196 -------ECSLAALMMRPAPILALTTAKLEEEEKEKGQEEQVPRVYIKTLLDRVMKPEQQ 255

Query: 258 EAMIREWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAAAAA 297
           +AMIR W P  VYE+++DHSPFFSNP +LFGLL+KAA +  +
Sbjct: 256 DAMIRRWPPSQVYELESDHSPFFSNPFVLFGLLIKAAVSVGS 275

BLAST of CmoCh15G001900 vs. TAIR 10
Match: AT5G58310.1 (methyl esterase 18 )

HSP 1 Score: 203.0 bits (515), Expect = 3.4e-52
Identity = 113/278 (40.65%), Postives = 157/278 (56.47%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG   GGWCWYK+   + ++G K TCIDLKGAGI+ +DPN+V   DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKNGVPD 141
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M++ G    + +KN +  
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 142 LS-----EFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEI 201
            S     E    +D  FG GPQ  PT+ ++K E  R   Y+ SP E              
Sbjct: 125 CSGLIEEETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPME-------------- 184

Query: 202 LSCLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEA 261
                   D TLA+ LLRP P++A           E D++ RVY++T  D + +P  QE 
Sbjct: 185 --------DYTLATTLLRPAPVMAFIGIMDIPGAPETDKIPRVYVKTGKDHLFEPVLQEV 244

Query: 262 MIREWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAAAA 294
           M+  W P   + + D+DHS FFS P  L+  L++AA++
Sbjct: 245 MLALWPPAHTFLLPDSDHSAFFSQPQELYQFLLQAASS 260

BLAST of CmoCh15G001900 vs. TAIR 10
Match: AT4G16690.1 (methyl esterase 16 )

HSP 1 Score: 196.8 bits (499), Expect = 2.5e-50
Identity = 109/275 (39.64%), Postives = 152/275 (55.27%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFV VHG S G WCWYK+  L++ +GFK T +DL GAGI L D N V   D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQDIKN---G 141
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G      + N   G
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPSPHLSNIHVG 131

Query: 142 VPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEILS 201
             D+ E+       +G+G  +PPT  ++K E  R   Y  SP E                
Sbjct: 132 EEDIWEY------TYGEGTDKPPTGVLMKPEFIRHYYYSQSPLE---------------- 191

Query: 202 CLEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMI 261
                 D TL+S LLRP P+ A +       N E ++V RVYI+T  D +    +Q+ ++
Sbjct: 192 ------DVTLSSKLLRPAPMRAFQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLV 251

Query: 262 REWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAAA 293
             W P  +Y + D+DHS FFS P+ LF  L++A +
Sbjct: 252 ENWPPSQLYVLEDSDHSAFFSVPTTLFAYLLRAVS 258

BLAST of CmoCh15G001900 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 159.1 bits (401), Expect = 5.7e-39
Identity = 93/287 (32.40%), Postives = 154/287 (53.66%), Query Frame = 0

Query: 9   PSAVGSVAVQKPPHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSV 68
           P    S+ V+K   FVLVHG   G WCWYK+   +E SG     +DL G G +++D N+V
Sbjct: 87  PDFSESLIVKK---FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTV 146

Query: 69  VKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFG 128
              ++Y+KPL+D + +LPE E++ILVGHS GG S++ A  +F +KI  A++V ATM+  G
Sbjct: 147 STLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDG 206

Query: 129 FQTDQDIKNGVPDLSEF-GDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFG 188
            +        +     F  ++  + +G G  +PPT  + ++   + + ++ SP++     
Sbjct: 207 QRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNK----- 266

Query: 189 IIPKSNHEILSCLEIFNDSTLASMLLRPGPI--LALRTARFREENGELDRVKRVYIRTTY 248
                            D  LA + +RP P+  +  + +   E  G   + +R Y++T  
Sbjct: 267 -----------------DIALAMISMRPVPLGPMMEKVSLTAERYG---KGRRFYVQTLD 326

Query: 249 DRVIKPEQQEAMIREWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAA 292
           DR + P+ QE ++RE  PE V+++  +DH PFFS P  L  +L++ A
Sbjct: 327 DRALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKILLEIA 345

BLAST of CmoCh15G001900 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 152.5 bits (384), Expect = 5.3e-37
Identity = 99/278 (35.61%), Postives = 133/278 (47.84%), Query Frame = 0

Query: 22  HFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLKGAGIDLSDPNSVVKFDDYNKPLLDF 81
           HFVLVHG S G WCWYK   L+E  GFKVT IDL G GI+  + N +     Y KPL D 
Sbjct: 138 HFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPLTDI 197

Query: 82  VSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLRFGFQTDQ--DIKNGV 141
           +  LP  E++ILVGH  GG  ++ A   F  KI  AV++AA ML  G  T     +K G 
Sbjct: 198 LEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQSTLDMFSLKAGQ 257

Query: 142 PDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIYHMSPHEFLSFGIIPKSNHEILSC 201
            DL      +   +  G + PPT   + + L + ++++ SP +                 
Sbjct: 258 NDLMRKAQIFI--YTNGNENPPTAIDLDKSLLKDLLFNQSPSK----------------- 317

Query: 202 LEIFNDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMIR 261
                D  LAS+ +R  P   +   +    +     V+R YI T  D  I    QE MI 
Sbjct: 318 -----DVALASVSMRSIPFAPV-LEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMIN 377

Query: 262 EWLPEIVYEM-DTDHSPFFSNPSLLFGLLVKAAAAAAA 297
              PE VY +   DH+PFFS P  L  LL++ A  + A
Sbjct: 378 SSPPEKVYRLKGADHAPFFSKPQALHKLLLEIARISPA 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SG923.3e-9257.09Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1[more]
Q2V0W19.8e-5240.44Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1[more]
Q9LVL94.9e-5140.65Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1[more]
O235123.5e-4939.64Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1[more]
Q9FVW38.0e-3832.40Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Match NameE-valueIdentityDescription
A0A6J1FL474.1e-15492.74methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1[more]
A0A6J1JV682.8e-15091.09methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1[more]
A0A5A7TBF55.1e-12877.89Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001... [more]
A0A1S3CAT25.1e-12877.89methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1[more]
A0A6J1BYB84.8e-12676.57methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022938905.18.5e-15492.74methylesterase 17 [Cucurbita moschata] >XP_023551105.1 methylesterase 17 [Cucurb... [more]
KAG6578491.12.1e-15292.43Methylesterase 17, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022993026.15.7e-15091.09methylesterase 17 [Cucurbita maxima][more]
KAG7016056.12.6e-14273.95Methylesterase 17, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038890470.17.0e-13281.16methylesterase 17 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G10870.12.4e-9357.09methyl esterase 17 [more]
AT5G58310.13.4e-5240.65methyl esterase 18 [more]
AT4G16690.12.5e-5039.64methyl esterase 16 [more]
AT1G33990.15.7e-3932.40methyl esterase 14 [more]
AT3G29770.15.3e-3735.61methyl esterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 23..280
e-value: 8.0E-13
score: 49.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 15..293
e-value: 4.8E-51
score: 176.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 19..288
NoneNo IPR availablePANTHERPTHR10992:SF1043METHYLESTERASE 17-LIKEcoord: 18..293
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 18..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G001900.1CmoCh15G001900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033473 indoleacetic acid conjugate metabolic process
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
biological_process GO:0048367 shoot system development
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity