CmoCh15G000340 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh15G000340
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	subtilisin-like protease SBT3.4
Location	Cmo_Chr15: 228372 .. 230047 (+)
RNA-Seq Expression	CmoCh15G000340
Synteny	CmoCh15G000340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGACGCATGGCGGAGTACGTTACCAGCGGCAAGGGCCTTATTGTTCCTCGTCGTGTCTTTCTTTACCATTTCCATATCCTCCGCCATGGCTGAGACCGATGAAACTTCTTCAACCGTTTCCATCGCTTCTTCGGCGCCGTCTTCACGGCCTGCAGTTCACATCGTGTACACCGAAAGTCCTCGGGATGAGGACCCCAAGGATTTCCATATCCGAACCCTAGCCTCTGCTCTCGGCAGGTTCGTCGCTTGTTTCTCTGTGCTCTTCTCATTAGTTTCAGTTCATGTTTTCGAAATGCAAAAGAAGTTAATCGGACAAATGCTAGCTCGTTTGCGTTTGCAGTCAGGAAGCTGCGAAGGATGCGCTTATTTACAGTTACAAAACGGCTGTTAGTGGATTCTCTGCAAAGCTCACTCCCGACCAGGTTTCTCGAGTTTCCCGTAAGTCTAGCTTCAGTAGTCGCGTTCTTGATTAGATAATTAAGTTATAAACATAGGAGACTGTTGCTACTGATTGTACTTCCTTCATCGTTTTCAGTGGATCGATTGACTAGTATACGAAATCGATTGTTTAAATTGGATCGAGGTTGATGTCTAGGGATTTATCTTCTTCACAACTCACATCATGCGTTTTAGTAAGATAGATCAAAGGATGACTCAAGCTTTGTGTAGCTTGCATCTTCTGCATTCATCGTTGTATCATTGTTTGTACTTTCCGTAGCAAAGGGCATCCCAAGTACATCCCATGTTTGGATTTAGGCTTCCAAAACCCACACCCATGCCCACCCATGGGTTACACCTCATTTCCATACGTTTGGGTTTCCTTCCTCTCTCCTTTATGTCATGTGTTTCATCTTCGTCCCTCCTGTGCTTACAGAAACTTCCCGTATTGAATGTTCCATGTATTTACAGAAATTTCTCTTTCAATTATTTTTTTTCTTCCTCATTCAATTTTCTTCTTATTTATGTGATAGCTACGAAATAATTCAAGATAAATAATATTTCAATTTTTTTTTTCAATAATCAATAAAAGAGAATTTGACAATTAGTTTCTTCATATAAATTACTCTTTAAAACCTGTGAATGTAGCGTTCACATTTAGCTTGTAAGTTATGGTAGGTAAAAAGGACAAAATCAATAGAGGTTACCCCCAGCAAGTTATCATCTTCCATTTAGCATTGTTCTGCTAATAAGTATCAGATTTCTCTGTAATTTTGGGTTTATATGTTTATAACTTTAGTAGTAAATTCAAATCTGGCTTCTTATCAACGAATACATCTGATTTATTAATTTAACATATACTTGAATAATTGCCTTCAAGATGGTGAAAGTCCTGTGTCAATTGTTGTCTAGTAGTGGCAATAATTGCCTTGATGTTGTTTTAGATTGATGTTCACTCATTAGCTGATAACAACCAAACAAATTTATCTGCTTTACTTGGCAGAACAACCAGGCGTTCTACAGGTTGTTCCAGACAGCCAGGTTGGGTTGCACCGTTAGAGGATGCTGTAGCCTCCCTTGAAGTTGGGTGAAGATGGCGTCATAATGAGTGAGTTTCATCTTCTGTAAACTCAGTTAAAGTTTTTTAGATTGCAACACTTGAATTGTTGTTTGTCAAGGCTGAATATTATCTCCATGTTGATACCCAACACTCAACTCTGTAAAAAGATCTAG

mRNA sequence

ATGATGGACGCATGGCGGAGTACGTTACCAGCGGCAAGGGCCTTATTGTTCCTCGTCGTGTCTTTCTTTACCATTTCCATATCCTCCGCCATGGCTGAGACCGATGAAACTTCTTCAACCGTTTCCATCGCTTCTTCGGCGCCGTCTTCACGGCCTGCAGTTCACATCGTGTACACCGAAAGTCCTCGGGATGAGGACCCCAAGGATTTCCATATCCGAACCCTAGCCTCTGCTCTCGGCAGTCAGGAAGCTGCGAAGGATGCGCTTATTTACAGTTACAAAACGGCTGTTAGTGGATTCTCTGCAAAGCTCACTCCCGACCAGGTTTCTCGAGTTTCCCAACAACCAGGCGTTCTACAGGTTGTTCCAGACAGCCAGTTAAAGTTTTTTAGATTGCAACACTTGAATTGTTGTTTGTCAAGGCTGAATATTATCTCCATGTTGATACCCAACACTCAACTCTGTAAAAAGATCTAG

Coding sequence (CDS)

ATGATGGACGCATGGCGGAGTACGTTACCAGCGGCAAGGGCCTTATTGTTCCTCGTCGTGTCTTTCTTTACCATTTCCATATCCTCCGCCATGGCTGAGACCGATGAAACTTCTTCAACCGTTTCCATCGCTTCTTCGGCGCCGTCTTCACGGCCTGCAGTTCACATCGTGTACACCGAAAGTCCTCGGGATGAGGACCCCAAGGATTTCCATATCCGAACCCTAGCCTCTGCTCTCGGCAGTCAGGAAGCTGCGAAGGATGCGCTTATTTACAGTTACAAAACGGCTGTTAGTGGATTCTCTGCAAAGCTCACTCCCGACCAGGTTTCTCGAGTTTCCCAACAACCAGGCGTTCTACAGGTTGTTCCAGACAGCCAGTTAAAGTTTTTTAGATTGCAACACTTGAATTGTTGTTTGTCAAGGCTGAATATTATCTCCATGTTGATACCCAACACTCAACTCTGTAAAAAGATCTAG

Protein sequence

MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTESPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVVPDSQLKFFRLQHLNCCLSRLNIISMLIPNTQLCKKI

Homology

[Close]

BLAST of CmoCh15G000340 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 5.7e-11
Identity = 36/83 (43.37%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 49  SSRPAVHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLT 108
           S++  VHIVY    + +DP+   + H R L S LGS+E A D+++YSY+   SGF+AKLT
Sbjct: 29  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 88

Query: 109 PDQVSRVSQQPGVLQVVPDSQLK 129
             Q  +++  P V+ V+PDS  K
Sbjct: 89  ESQAKKIADLPDVVHVIPDSFYK 111

[Close]

BLAST of CmoCh15G000340 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.4e-11
Identity = 33/80 (41.25%), Postives = 53/80 (66.25%), Query Frame = 0


Query: 49  SSRPAVHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLT 108
           S++  VHIVY    + +DPK   + H + L+S LGS++ A ++++YSY+   SGF+AKLT
Sbjct: 24  SAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLT 83

Query: 109 PDQVSRVSQQPGVLQVVPDS 126
             Q  +++  P V+ V+PDS
Sbjct: 84  KSQAKKIADSPEVIHVIPDS 103

[Close]

BLAST of CmoCh15G000340 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.4e-09
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 54  VHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVS 113
           VHIVY       DP+     H+R L S LGS++ A +++++SY+   SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

Query: 114 RVSQQPGVLQVVPDSQLKFFRLQ 134
           ++S+ P V+QV P++   F+ LQ
Sbjct: 97  QISEHPDVVQVTPNT---FYELQ 116

[Close]

BLAST of CmoCh15G000340 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.3e-09
Identity = 37/89 (41.57%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 40  TVSIASSAPS-SRPAVHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKT 99
           TV  A+ A S +   VHIVY    +  DP+   + H + LAS LGS++ A D+++YSY+ 
Sbjct: 17  TVLNATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRH 76

Query: 100 AVSGFSAKLTPDQVSRVSQQPGVLQVVPD 125
             SGF+AKLT  Q  +++  P V+ V+PD
Sbjct: 77  GFSGFAAKLTKSQAKKIADLPEVVHVIPD 105

[Close]

BLAST of CmoCh15G000340 vs. ExPASy Swiss-Prot
Match: Q9C7U8 (Subtilisin-like protease SBT3.17 OS=Arabidopsis thaliana OX=3702 GN=SBT3.17 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.2e-08
Identity = 32/78 (41.03%), Postives = 49/78 (62.82%), Query Frame = 0


Query: 54  VHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVS 113
           +HIV+  + + + P+     H + L   LGS+EAAK++L+Y+YK   SGF+AKLT  Q  
Sbjct: 37  IHIVHLGAKQHDTPELVTKSHYQILEPLLGSKEAAKNSLVYNYKHGFSGFAAKLTASQAK 96

Query: 114 RVSQQPGVLQVVPDSQLK 129
            +S  P VL+VVP   ++
Sbjct: 97  NLSAHPEVLRVVPSRVMR 114

[Close]

BLAST of CmoCh15G000340 vs. ExPASy TrEMBL
Match: A0A6J1JST0 (subtilisin-like protease SBT3.4 OS=Cucurbita maxima OX=3661 GN=LOC111489448 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.5e-54
Identity = 121/131 (92.37%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTE 60
           MMDAWR+ LPAARALLFL VSFFTISISSAMAETDETSSTVSIASSA SSR AVHIVYTE
Sbjct: 1   MMDAWRNKLPAARALLFLFVSFFTISISSAMAETDETSSTVSIASSAASSRSAVHIVYTE 60

Query: 61  SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120
           SPRDEDPKDFHIRTLASALGS+EAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ
Sbjct: 61  SPRDEDPKDFHIRTLASALGSEEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120

Query: 121 VVPDSQLKFFR 132
           VVPDSQ+   R
Sbjct: 121 VVPDSQVGLHR 131

[Close]

BLAST of CmoCh15G000340 vs. ExPASy TrEMBL
Match: A0A1S3B2X4 (subtilisin-like protease SBT3.6 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103485421 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 7.2e-41
Identity = 94/117 (80.34%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 15  LLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTESPRDEDPKDFHIRT 74
           LLF++VSFF +SIS+AMA++ E SST SIASS PSS PAVHIVYTE+P DE+PKDFHIRT
Sbjct: 9   LLFVLVSFFAVSISTAMADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHIRT 68

Query: 75  LASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVVPDSQLKFFR 132
           LASALGS+EAAKDAL+YSYKTAV GFSAKLTPDQVSRVSQQPGVLQV+PDSQ+   R
Sbjct: 69  LASALGSEEAAKDALVYSYKTAVGGFSAKLTPDQVSRVSQQPGVLQVIPDSQVGLHR 125

[Close]

BLAST of CmoCh15G000340 vs. ExPASy TrEMBL
Match: A0A6J1DGF7 (subtilisin-like protease SBT3.3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020217 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 7.9e-40
Identity = 92/118 (77.97%), Postives = 104/118 (88.14%), Query Frame = 0


Query: 14  ALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTESPRDEDPKDFHIR 73
           +LL L+VSFF ISIS+AMAE DETS+T S  SS+ SSRPAVHIVYTE+P DE+P+ FHIR
Sbjct: 9   SLLLLLVSFFAISISAAMAEADETSATASTVSSSASSRPAVHIVYTETPTDEEPEHFHIR 68

Query: 74  TLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVVPDSQLKFFR 132
           TLAS LGS+EAAKDALIYSYK+A  GFSAKLTPDQVSR+SQQPGVLQVVPDSQL+  R
Sbjct: 69  TLASVLGSEEAAKDALIYSYKSAARGFSAKLTPDQVSRISQQPGVLQVVPDSQLELHR 126

[Close]

BLAST of CmoCh15G000340 vs. ExPASy TrEMBL
Match: A0A1S3B2Y7 (subtilisin-like protease SBT3.6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485421 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 3.9e-39
Identity = 93/118 (78.81%), Postives = 106/118 (89.83%), Query Frame = 0


Query: 15  LLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTESPRDEDPKDFHIRT 74
           LLF++VSFF +SIS+AMA++ E SST SIASS PSS PAVHIVYTE+P DE+PKDFHIRT
Sbjct: 9   LLFVLVSFFAVSISTAMADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHIRT 68

Query: 75  LASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVS-QQPGVLQVVPDSQLKFFR 132
           LASALGS+EAAKDAL+YSYKTAV GFSAKLTPDQVSRVS +QPGVLQV+PDSQ+   R
Sbjct: 69  LASALGSEEAAKDALVYSYKTAVGGFSAKLTPDQVSRVSPEQPGVLQVIPDSQVGLHR 126

[Close]

BLAST of CmoCh15G000340 vs. ExPASy TrEMBL
Match: A0A0A0LMH0 (Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402130 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 3.4e-35
Identity = 85/103 (82.52%), Postives = 95/103 (92.23%), Query Frame = 0


Query: 13  RALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTESPRDEDPKDFHI 72
           + LLF++VSFF ISIS AMA++DE SST SIASS PSSRPAVHIVYTE+P DEDPK FHI
Sbjct: 7   KKLLFVLVSFFAISISIAMADSDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHI 66

Query: 73  RTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQ 116
           RTLASALGS+EAAKDAL+YSYKTAVSGFSAKLTPDQVSRVS++
Sbjct: 67  RTLASALGSEEAAKDALVYSYKTAVSGFSAKLTPDQVSRVSRE 109

[Close]

BLAST of CmoCh15G000340 vs. NCBI nr
Match: KAG7015906.1 (Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235.0 bits (598), Expect = 4.6e-58
Identity = 127/131 (96.95%), Postives = 128/131 (97.71%), Query Frame = 0


Query: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTE 60
           MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTE
Sbjct: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTE 60

Query: 61  SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120
           SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ
Sbjct: 61  SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120

Query: 121 VVPDSQLKFFR 132
           VVPDSQ+   R
Sbjct: 121 VVPDSQVGLHR 131

[Close]

BLAST of CmoCh15G000340 vs. NCBI nr
Match: KAG6578330.1 (Subtilisin-like protease 3.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 231.9 bits (590), Expect = 3.9e-57
Identity = 126/131 (96.18%), Postives = 127/131 (96.95%), Query Frame = 0


Query: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTE 60
           MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSA SSRPAVHIVYTE
Sbjct: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAASSRPAVHIVYTE 60

Query: 61  SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120
           SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ
Sbjct: 61  SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120

Query: 121 VVPDSQLKFFR 132
           VVPDSQ+   R
Sbjct: 121 VVPDSQVGLHR 131

[Close]

BLAST of CmoCh15G000340 vs. NCBI nr
Match: XP_023550028.1 (subtilisin-like protease SBT3.4 [Cucurbita pepo subsp. pepo] >XP_023550029.1 subtilisin-like protease SBT3.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.9 bits (572), Expect = 4.7e-55
Identity = 121/131 (92.37%), Postives = 125/131 (95.42%), Query Frame = 0


Query: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTE 60
           M DAWRS LPAARALLFL VSFFTISISSAMAETDETSSTVSIASSA SSRPAVHIVYTE
Sbjct: 1   MKDAWRSKLPAARALLFLFVSFFTISISSAMAETDETSSTVSIASSAASSRPAVHIVYTE 60

Query: 61  SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120
           SPRDEDPKDFHIRTLASALGS+EAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ
Sbjct: 61  SPRDEDPKDFHIRTLASALGSEEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120

Query: 121 VVPDSQLKFFR 132
           V+PDSQ++  R
Sbjct: 121 VIPDSQVELHR 131

[Close]

BLAST of CmoCh15G000340 vs. NCBI nr
Match: XP_022993431.1 (subtilisin-like protease SBT3.4 [Cucurbita maxima] >XP_022993432.1 subtilisin-like protease SBT3.4 [Cucurbita maxima])

HSP 1 Score: 222.2 bits (565), Expect = 3.1e-54
Identity = 121/131 (92.37%), Postives = 124/131 (94.66%), Query Frame = 0


Query: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTE 60
           MMDAWR+ LPAARALLFL VSFFTISISSAMAETDETSSTVSIASSA SSR AVHIVYTE
Sbjct: 1   MMDAWRNKLPAARALLFLFVSFFTISISSAMAETDETSSTVSIASSAASSRSAVHIVYTE 60

Query: 61  SPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120
           SPRDEDPKDFHIRTLASALGS+EAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ
Sbjct: 61  SPRDEDPKDFHIRTLASALGSEEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQ 120

Query: 121 VVPDSQLKFFR 132
           VVPDSQ+   R
Sbjct: 121 VVPDSQVGLHR 131

[Close]

BLAST of CmoCh15G000340 vs. NCBI nr
Match: XP_038885185.1 (subtilisin-like protease SBT3.4 isoform X2 [Benincasa hispida])

HSP 1 Score: 184.9 bits (468), Expect = 5.4e-43
Identity = 105/133 (78.95%), Postives = 113/133 (84.96%), Query Frame = 0


Query: 1   MMDAWRSTLPAARALLFLVVSFFTISISSAMAETDETSSTV--SIASSAPSSRPAVHIVY 60
           MM+A R  L AA   LF+ VS F ISIS+AMA++DE SST   SIASS  SSRPAVHIVY
Sbjct: 2   MMNAKRKKLRAATTFLFVFVSSFAISISAAMADSDEISSTSTHSIASSTASSRPAVHIVY 61

Query: 61  TESPRDEDPKDFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGV 120
           TE+PRDEDPKDFHIRTLASALGS+EAAKDALIYSYKTA SGFSAKLTPDQVSRVSQQPGV
Sbjct: 62  TETPRDEDPKDFHIRTLASALGSEEAAKDALIYSYKTAASGFSAKLTPDQVSRVSQQPGV 121

Query: 121 LQVVPDSQLKFFR 132
           LQVVPDSQ+   R
Sbjct: 122 LQVVPDSQVGLHR 134

[Close]

BLAST of CmoCh15G000340 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 115.5 bits (288), Expect = 3.8e-26
Identity = 63/108 (58.33%), Postives = 78/108 (72.22%), Query Frame = 0


Query: 16  LFLVVSFFTISISSAMAETDETSSTVSIASSAPSSRPAVHIVYTESPRDEDPKDFHIRTL 75
           L ++V FF   +SS  A           A+   SS   VHI+YTE P DE+PK +H+RTL
Sbjct: 13  LIIIVFFFFFCLSSFFASIVMADEYTGEATG--SSEAKVHIIYTEKPTDEEPKTYHLRTL 72

Query: 76  ASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVSRVSQQPGVLQVVP 124
           +SALGS+EAAKDALIYSYK A SGFSAKLTP+QV+ +S+QPGV+QVVP
Sbjct: 73  SSALGSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVP 118

[Close]

BLAST of CmoCh15G000340 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 68.9 bits (167), Expect = 4.1e-12
Identity = 36/83 (43.37%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 49  SSRPAVHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLT 108
           S++  VHIVY    + +DP+   + H R L S LGS+E A D+++YSY+   SGF+AKLT
Sbjct: 29  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 88

Query: 109 PDQVSRVSQQPGVLQVVPDSQLK 129
             Q  +++  P V+ V+PDS  K
Sbjct: 89  ESQAKKIADLPDVVHVIPDSFYK 111

[Close]

BLAST of CmoCh15G000340 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 68.9 bits (167), Expect = 4.1e-12
Identity = 36/83 (43.37%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 49  SSRPAVHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLT 108
           S++  VHIVY    + +DP+   + H R L S LGS+E A D+++YSY+   SGF+AKLT
Sbjct: 45  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 104

Query: 109 PDQVSRVSQQPGVLQVVPDSQLK 129
             Q  +++  P V+ V+PDS  K
Sbjct: 105 ESQAKKIADLPDVVHVIPDSFYK 127

[Close]

BLAST of CmoCh15G000340 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 68.6 bits (166), Expect = 5.3e-12
Identity = 33/80 (41.25%), Postives = 53/80 (66.25%), Query Frame = 0


Query: 49  SSRPAVHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLT 108
           S++  VHIVY    + +DPK   + H + L+S LGS++ A ++++YSY+   SGF+AKLT
Sbjct: 24  SAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLT 83

Query: 109 PDQVSRVSQQPGVLQVVPDS 126
             Q  +++  P V+ V+PDS
Sbjct: 84  KSQAKKIADSPEVIHVIPDS 103

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BLAST of CmoCh15G000340 vs. TAIR 10
Match: AT5G11940.1 (Subtilase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 1.7e-10
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 54  VHIVYTESPRDEDPK---DFHIRTLASALGSQEAAKDALIYSYKTAVSGFSAKLTPDQVS 113
           VHIVY       DP+     H+R L S LGS++ A +++++SY+   SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

Query: 114 RVSQQPGVLQVVPDSQLKFFRLQ 134
           ++S+ P V+QV P++   F+ LQ
Sbjct: 97  QISEHPDVVQVTPNT---FYELQ 116

The following BLAST results are available for this feature:

BLAST of CmoCh15G000340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7I2	5.7e-11	43.37	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
F4HPF1	7.4e-11	41.25	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q1PDX5	2.4e-09	39.76	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]
Q9MAP5	5.3e-09	41.57	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
Q9C7U8	1.2e-08	41.03	Subtilisin-like protease SBT3.17 OS=Arabidopsis thaliana OX=3702 GN=SBT3.17 PE=3...	[more]

BLAST of CmoCh15G000340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JST0	1.5e-54	92.37	subtilisin-like protease SBT3.4 OS=Cucurbita maxima OX=3661 GN=LOC111489448 PE=4...	[more]
A0A1S3B2X4	7.2e-41	80.34	subtilisin-like protease SBT3.6 isoform X3 OS=Cucumis melo OX=3656 GN=LOC1034854...	[more]
A0A6J1DGF7	7.9e-40	77.97	subtilisin-like protease SBT3.3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A1S3B2Y7	3.9e-39	78.81	subtilisin-like protease SBT3.6 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034854...	[more]
A0A0A0LMH0	3.4e-35	82.52	Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40213...	[more]

BLAST of CmoCh15G000340 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015906.1	4.6e-58	96.95	Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6578330.1	3.9e-57	96.18	Subtilisin-like protease 3.6, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023550028.1	4.7e-55	92.37	subtilisin-like protease SBT3.4 [Cucurbita pepo subsp. pepo] >XP_023550029.1 sub...	[more]
XP_022993431.1	3.1e-54	92.37	subtilisin-like protease SBT3.4 [Cucurbita maxima] >XP_022993432.1 subtilisin-li...	[more]
XP_038885185.1	5.4e-43	78.95	subtilisin-like protease SBT3.4 isoform X2 [Benincasa hispida]	[more]

BLAST of CmoCh15G000340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	3.8e-26	58.33	Proteinase inhibitor, propeptide	[more]
AT4G10550.1	4.1e-12	43.37	Subtilase family protein	[more]
AT4G10550.3	4.1e-12	43.37	Subtilase family protein	[more]
AT1G32950.1	5.3e-12	41.25	Subtilase family protein	[more]
AT5G11940.1	1.7e-10	39.76	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 36..130 e-value: 1.4E-20 score: 75.5
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 67..128 e-value: 1.7E-12 score: 47.8
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 17..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh15G000340.1	CmoCh15G000340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity