CmoCh15G000140 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G000140
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionTubulin-folding cofactor E
LocationCmo_Chr15: 102404 .. 106738 (+)
RNA-Seq ExpressionCmoCh15G000140
SyntenyCmoCh15G000140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAACTAATACTTCGAATGATCCAATTCTACCATACATTCTTCCATCTCCATGCGGAGGAGAAAAGATGCAAGGCTCAATTCAGCTTCAGCTGGAGTTCAGGCTGGGCCAGCGAGTCCACTTTGTCGGTGATCCCCGACGTGTCGGAACAGTGGCTTTCGTAGGAACCCTGGAAGGATATTCAGGAACCTGGATCGGAGTGGATTGGGACGACAGCAATGGCAAGCACGACGGCTCCATCAACGGGGTCCGGTATTTCCAGGCAAAGTCAGAACGATCTGGCTCTTTTGTTCGGGCTCAGAATCTGAGTTTCGGCGTTTCGCTTCTCCAAGCGTTGGAGCTCAGATATAGAGGCGACTCTACCAAAGAAGAAGAGGGTATGTATCTGCGCTATCAATATCTGTCTAGTTGCTCCTAACATGGCTGCCTAATTGTGGTATATCGTACACTTAATCTTACTGCCTAAACTCAAATTTTATTTTCAACTTGTTTAATGTAATTCAACGGTTGCGCTGAATTTGTTGGTAATGGGTTGTCCTGCGTTGCCATTTGCTCTCTTGCAGATGAAATGTACGTACTCTCAGCCAGTGACAAGCGTGTATCTGTTCAATTCGTGGGTAAAGACCTTATTAAAGACAAGCTTAGTCGTTTTGAGGAGTTAACTAGTGTATCGCTGTCTTATATGGGTGTTAGCTCTCCTGGAAATCCATGTCAGATTGGCTCTGTCTTGCCAAGTAAGTTTCTTGACCTCATTGAAGTATACATTTGTCTTCTTAACTTCAAATACGTTGTTGATTTTCATGATGCGGTTTATATGAGAAACATTGTATTAAACCAGCGTAGCAAAAGACCACACTCAGACATTTGGAGCTGAAGCATTTAATCATTGATGATCACCCTTTTAAAGTTGATGCCAAGCTTCTTTTGATGAATTTTACGAGATCTTTCTTTGGAATGTTTGTGTAAAGAAACAATAAAAATTTTGGGAGAGAAAAGATATAGGATCACTGTTTTATGGGATAATCTTTTGTCCTGTCGCTATTTATAATTCTAATGTGATTATATATGTTTCATCTGTAATGTCTCCATTTCAGACGTAAAGCAGCTCGACCTTACTGGAAATTTGCTATCAGATTGGAAGGTACGTGTTGAAGAAAACCATTATTGTTCACCCTCTTAGCAACCCACACTGAAAATGTACAGTCATAACTTGCAATTAACTGTGGTTTTAAACACCAGTCTTCGACTCCACCATAGAGTTTTTGTTGTTTTTTCAGAAAATACTTGGGTTTATGTTACGTTGATCAGTTGTTTACCTCTTCAGGGTGAAAACTTGGTAGAACACATTGTGTTCCTTTTTTCGCTTGAAAGATGAGGGTTGTCCTTTGCTTTTTAATATGTGGTTGTATAAATTCAATCAGTTCGACGTATATGTGCCTTTTTTCAATGGCCAAACCAGTGAATTCTTAACGTAAATGTCTTTGAGGTTTGTTCTGTATCGTGCCCTGTGGGAAGGTCTGATACCCTGCTGAACAATTTTTTCGATCTAGTCCCATCAAAGAAATAAAAAAGATGGAAAGAAAGAAAGAACGAGCATCTTTCTTTTGTTTTCCAAATTGTAATTTCTTTGTTTGTTGGCAGTTTAGTAGTTCTGAACATTATTGTCTTATTGTCAACTTCATATATTCATGCAGGATATTAGCATCATTTGTGACCAATTACAAGCTCTTGTAGCCCTCATTTTATCGAACAACTTATTGTCACGTGATATCTCAGGGCCTGTTCAATTGAATCACATTCGTATTTTAGTTCTAAACAATACTGGAATAACTTGGATGCAGGTTAGTTGTTAGTATGGTTGAGATATATATATTTTTTTTCTTCTCCTTATCCTTTATATATTCATATTATATGTGGAGTTAATTTTATATGATAGGTTGAAACACTTGAACATTCTCTGCCAACAATCGAAGAACTACATCTGATGGGAAATAAAATAAGCACAGTAACGGTACTGATTGAGATCCTTAAACTTTGTAATTGTTGGATGAATACATAATAATTTAGTCTCTTGTTGCAGTTGCATTGCATTGCATTGCATTGATTATTGATTTCATCTGATGGGAAATAATTTAGTCTTTCAATGCTTGTGAGTTGCAGAAATGAATCGAATAATAGAAGGGTTTATAAAGTCGATTAATCCGTTTACAATTGTGTCACTTAGTTCTATAATACGAACAACTAACCTGTATTTCTGTTGAGATCTCGTTTTCAGCCAGAATCATCATCCCCTATGGTCCAAGGATTTAATCTTCTGCGACTTCTGAATCTGGAAAATAACTGTATAGCTGAGTGGAGCGAAATATTGAAACTGGGTCAATTAAGAAGGTATTGTTGTTTTATTATTATTTTGTTCTTCATTGGGACAATTGGCTGTTGTCGAACCCTCGCTTATTTCACTGTATTTCCTTTGAATCTTTGACTATGCTATCAGCTTGAAGCAGATTCAGTTGAATAACAACAAATTGAGTCGTATATTTTATCCTAGCTTGGATGAATTACACAAGTTATTTGGAGATGTTGAACCACAAGGAGACTGTTTTCCATTCCGAAATTTGCGAAACCTCTTTCTTGGTACATTATCAAGGTTCTTTAACAATTGTGATCTCTTTATAGATTCTTTTAATTACTTCTCTGACTTGTTCTATTTCTGTATGTACTTCAGGAGGCAACAACATCGATGATTTGGCCTCTATCGACTTTTTAAACTCGTTTCCTCAGTTGATTGTAAGATTTTAATATATATTATTTCAATACGAGGGATAGTTCTTGAATTGAAAAACCTAATAACATTGGCAGATACATTATCGACTCGAACTTCAAGTTCTTTATCGTTTTATTTATTCATTTCTTTTTCCTTCAAGGACATCAGGCTATCAGAGAATCCAATAGCTGATCCCATGAGGGGTGGGATTCCCAGATATGTTTTAATTGCGCGATTGTCTAAAGTTCAAATAATCAATGGGAGTGAGGTTGTTTGGAAGCATTTGCTGTCCTTTTCTTTTTCTCATTATTATATTATCGTTAACCCGAAAACTTTTTTCTCATATATTTTTTAGGTAACTCCTCGTGAAAGAAGAGACTCTGAAATACGGTGCGGAAATCAATATTTTAGATGCATTATTAGTTTCCTCCCCTTCTTTGTACTAACAAGGATATATTGGTAATATGAGTAGTCTTGATATAACCAGAGGGAGTAAGGGTGCGAGTCTATAGTCTATGTTTAAGATGTGAAGTTATTATGAAGAACATTTGAATCCTTTCAGGAAACTGTGGTTCATTCGAAGTTATAAATTTCGAACATTTGTATCCGTTTATTCAGTGATTTCAATTCTTCTTGCAGCTACGTACGCATGGTTATGTCAAAGTTGGATAGCAATCCTGAGGAAACATTAAGGCTACATCCTAGGTAACTGCATTCAACTAAAACTGAACTTACCCCGTGCTGCGCCCCGGGGAACGTTATTTTTGTTGATATATTTATGCATCAGGTTTGAAGAGCTTAAGAGGTTTTATGGGATCGAAGATAATAGGGTATCGGTCGGAGCTGGTGGACCTCAGAAACTGGCATCTGGGCTCATATGTAGGAATGAATCTTGAATTTGAGATATTTGACATTTTAGAACATTTCCTTATCGTCGCTGAATAGTTGTTTGATAAATTTATCCAGCAATCACCCTGAATTGCGTGGGAGCATCAATCGGGGAGAAGCCACCATTGACAAAGAAATTACCACTCACTACTACAGTTACTGTCCTTGCTTTTTTATTTTAACATGCTGTTTCACTGCTTCACTCTAAATGACTCTTTCCATCTTTAGGTTGGCAAGTTAAAGATGCTTTGTGAAAGCTTTTTCAAATTAAAATGTATGAGGCTAAAGTTGTATCTTCAAGAACAGGTTTGGTCTCCTAATTACTCAATACCCAACTCATTAACCTGCTGCAACTGAACACACCTTACATTCTCAGGATTCCCCCGTGCCGATACCGCTCGAGGACGATATGGCATCTCTTATGGATCTTGGGGTTGCTAATGGATCCAACATTCTTGTGGATGAAGAGAGTTGAAGGAAATAAACCCCCCATAGGTTCTTCAATTTTTTTCCTTTTTCCTTCTTTCTGGCATTCCCATGATTCCTGCTCCCCAGATTTGGAAGATGTTTTTAATTGTTGGTTCTCTACCGGATGTTCTTATATCATTTGACCAAGATTCACAGCTTAAACAAATCGTGAAATCACACTGAATTGCCTGATCTTCTATTCTATTTATCTGCCTC

mRNA sequence

ATAACTAATACTTCGAATGATCCAATTCTACCATACATTCTTCCATCTCCATGCGGAGGAGAAAAGATGCAAGGCTCAATTCAGCTTCAGCTGGAGTTCAGGCTGGGCCAGCGAGTCCACTTTGTCGGTGATCCCCGACGTGTCGGAACAGTGGCTTTCGTAGGAACCCTGGAAGGATATTCAGGAACCTGGATCGGAGTGGATTGGGACGACAGCAATGGCAAGCACGACGGCTCCATCAACGGGGTCCGGTATTTCCAGGCAAAGTCAGAACGATCTGGCTCTTTTGTTCGGGCTCAGAATCTGAGTTTCGGCGTTTCGCTTCTCCAAGCGTTGGAGCTCAGATATAGAGGCGACTCTACCAAAGAAGAAGAGGATGAAATGTACGTACTCTCAGCCAGTGACAAGCGTGTATCTGTTCAATTCGTGGGTAAAGACCTTATTAAAGACAAGCTTAGTCGTTTTGAGGAGTTAACTAGTGTATCGCTGTCTTATATGGGTGTTAGCTCTCCTGGAAATCCATGTCAGATTGGCTCTGTCTTGCCAAACGTAAAGCAGCTCGACCTTACTGGAAATTTGCTATCAGATTGGAAGGATATTAGCATCATTTGTGACCAATTACAAGCTCTTGTAGCCCTCATTTTATCGAACAACTTATTGTCACGTGATATCTCAGGGCCTGTTCAATTGAATCACATTCGTATTTTAGTTCTAAACAATACTGGAATAACTTGGATGCAGGTTGAAACACTTGAACATTCTCTGCCAACAATCGAAGAACTACATCTGATGGGAAATAAAATAAGCACAGTAACGCCAGAATCATCATCCCCTATGGTCCAAGGATTTAATCTTCTGCGACTTCTGAATCTGGAAAATAACTGTATAGCTGAGTGGAGCGAAATATTGAAACTGGGTCAATTAAGAAGCTTGAAGCAGATTCAGTTGAATAACAACAAATTGAGTCGTATATTTTATCCTAGCTTGGATGAATTACACAAGTTATTTGGAGATGTTGAACCACAAGGAGACTGTTTTCCATTCCGAAATTTGCGAAACCTCTTTCTTGGAGGCAACAACATCGATGATTTGGCCTCTATCGACTTTTTAAACTCGTTTCCTCAGTTGATTGACATCAGGCTATCAGAGAATCCAATAGCTGATCCCATGAGGGGTGGGATTCCCAGATATGTTTTAATTGCGCGATTGTCTAAAGTTCAAATAATCAATGGGAGTGAGGTAACTCCTCGTGAAAGAAGAGACTCTGAAATACGCTACGTACGCATGGTTATGTCAAAGTTGGATAGCAATCCTGAGGAAACATTAAGGCTACATCCTAGGTTTGAAGAGCTTAAGAGGTTTTATGGGATCGAAGATAATAGGGTATCGGTCGGAGCTGGTGGACCTCAGAAACTGGCATCTGGGCTCATATCAATCACCCTGAATTGCGTGGGAGCATCAATCGGGGAGAAGCCACCATTGACAAAGAAATTACCACTCACTACTACAGTTGGCAAGTTAAAGATGCTTTGTGAAAGCTTTTTCAAATTAAAATGTATGAGGCTAAAGTTGTATCTTCAAGAACAGGATTCCCCCGTGCCGATACCGCTCGAGGACGATATGGCATCTCTTATGGATCTTGGGGTTGCTAATGGATCCAACATTCTTGTGGATGAAGAGAGTTGAAGGAAATAAACCCCCCATAGGTTCTTCAATTTTTTTCCTTTTTCCTTCTTTCTGGCATTCCCATGATTCCTGCTCCCCAGATTTGGAAGATGTTTTTAATTGTTGGTTCTCTACCGGATGTTCTTATATCATTTGACCAAGATTCACAGCTTAAACAAATCGTGAAATCACACTGAATTGCCTGATCTTCTATTCTATTTATCTGCCTC

Coding sequence (CDS)

ATGCAAGGCTCAATTCAGCTTCAGCTGGAGTTCAGGCTGGGCCAGCGAGTCCACTTTGTCGGTGATCCCCGACGTGTCGGAACAGTGGCTTTCGTAGGAACCCTGGAAGGATATTCAGGAACCTGGATCGGAGTGGATTGGGACGACAGCAATGGCAAGCACGACGGCTCCATCAACGGGGTCCGGTATTTCCAGGCAAAGTCAGAACGATCTGGCTCTTTTGTTCGGGCTCAGAATCTGAGTTTCGGCGTTTCGCTTCTCCAAGCGTTGGAGCTCAGATATAGAGGCGACTCTACCAAAGAAGAAGAGGATGAAATGTACGTACTCTCAGCCAGTGACAAGCGTGTATCTGTTCAATTCGTGGGTAAAGACCTTATTAAAGACAAGCTTAGTCGTTTTGAGGAGTTAACTAGTGTATCGCTGTCTTATATGGGTGTTAGCTCTCCTGGAAATCCATGTCAGATTGGCTCTGTCTTGCCAAACGTAAAGCAGCTCGACCTTACTGGAAATTTGCTATCAGATTGGAAGGATATTAGCATCATTTGTGACCAATTACAAGCTCTTGTAGCCCTCATTTTATCGAACAACTTATTGTCACGTGATATCTCAGGGCCTGTTCAATTGAATCACATTCGTATTTTAGTTCTAAACAATACTGGAATAACTTGGATGCAGGTTGAAACACTTGAACATTCTCTGCCAACAATCGAAGAACTACATCTGATGGGAAATAAAATAAGCACAGTAACGCCAGAATCATCATCCCCTATGGTCCAAGGATTTAATCTTCTGCGACTTCTGAATCTGGAAAATAACTGTATAGCTGAGTGGAGCGAAATATTGAAACTGGGTCAATTAAGAAGCTTGAAGCAGATTCAGTTGAATAACAACAAATTGAGTCGTATATTTTATCCTAGCTTGGATGAATTACACAAGTTATTTGGAGATGTTGAACCACAAGGAGACTGTTTTCCATTCCGAAATTTGCGAAACCTCTTTCTTGGAGGCAACAACATCGATGATTTGGCCTCTATCGACTTTTTAAACTCGTTTCCTCAGTTGATTGACATCAGGCTATCAGAGAATCCAATAGCTGATCCCATGAGGGGTGGGATTCCCAGATATGTTTTAATTGCGCGATTGTCTAAAGTTCAAATAATCAATGGGAGTGAGGTAACTCCTCGTGAAAGAAGAGACTCTGAAATACGCTACGTACGCATGGTTATGTCAAAGTTGGATAGCAATCCTGAGGAAACATTAAGGCTACATCCTAGGTTTGAAGAGCTTAAGAGGTTTTATGGGATCGAAGATAATAGGGTATCGGTCGGAGCTGGTGGACCTCAGAAACTGGCATCTGGGCTCATATCAATCACCCTGAATTGCGTGGGAGCATCAATCGGGGAGAAGCCACCATTGACAAAGAAATTACCACTCACTACTACAGTTGGCAAGTTAAAGATGCTTTGTGAAAGCTTTTTCAAATTAAAATGTATGAGGCTAAAGTTGTATCTTCAAGAACAGGATTCCCCCGTGCCGATACCGCTCGAGGACGATATGGCATCTCTTATGGATCTTGGGGTTGCTAATGGATCCAACATTCTTGTGGATGAAGAGAGTTGA

Protein sequence

MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSINGVRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELHLMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLSRIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Homology
BLAST of CmoCh15G000140 vs. ExPASy Swiss-Prot
Match: Q8GRL7 (Tubulin-folding cofactor E OS=Arabidopsis thaliana OX=3702 GN=TFCE PE=2 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 3.5e-185
Identity = 335/530 (63.21%), Postives = 412/530 (77.74%), Query Frame = 0

Query: 11  FRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWD-DSNGKHDGSINGVRYFQAKSE 70
           F +GQRVH + D RRVGTV +VG +EGYSGTWIGVDWD D +GKH+GS+NGV YF  +S+
Sbjct: 10  FIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYFNGRSQ 69

Query: 71  RSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDK 130
            S SFVR+QNLS G++LLQALELRYR  STK+EEDEMYVLSA ++RVS+Q +G D I+DK
Sbjct: 70  SSASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGGDKIQDK 129

Query: 131 LSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALV 190
           LSRFEELTS SLSY+GVSS G    +GS+LPN+K LDLTGNL+SDW++I  +C+QL AL 
Sbjct: 130 LSRFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCEQLPALT 189

Query: 191 ALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELHLMGNKISTV 250
            L LS N LS DI    QL +IR+LVLNN+G++W QVE L  SLP IEELHLMGN IST+
Sbjct: 190 TLNLSCNSLSSDIKSLPQLKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMGNMISTI 249

Query: 251 TPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLSRIFYPSLDE 310
           T  SSS   Q FN LRLLNL++NCI++WSE+LKL QL  L+Q+ LN NKLSRIF      
Sbjct: 250 TSTSSSD-DQAFNSLRLLNLDDNCISDWSEVLKLSQLPCLEQLYLNKNKLSRIF------ 309

Query: 311 LHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPMR 370
              + G    +    PF +L  L LG NNI DLAS+D LN FPQL+DIRLSENPI+DP+R
Sbjct: 310 -QSVNGTESSEKGSDPFPSLSCLLLGANNIGDLASVDALNGFPQLVDIRLSENPISDPVR 369

Query: 371 GGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETLRLHPRFEEL 430
           GG+PR+VL+ARL+KVQ++NGSEV  RE++DSEIRYVRMVMSKL+    E   LHPRF EL
Sbjct: 370 GGVPRFVLVARLTKVQVLNGSEVRAREKKDSEIRYVRMVMSKLNDKSGEIELLHPRFYEL 429

Query: 431 KRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLC 490
           K+ +GIED R S    GP+ +ASGLISITL CVG S+GEKP LTKKLP + TVGKLK+L 
Sbjct: 430 KKLHGIEDERASAENSGPKNIASGLISITLKCVGPSMGEKPHLTKKLPGSITVGKLKILS 489

Query: 491 ESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           E+FFKLK ++ +L+LQE+ SP P  L+D+ A+L+D+G+ +GS +LVDEES
Sbjct: 490 ENFFKLKSIKPRLFLQEEGSPFPTALDDETATLLDVGICDGSTLLVDEES 531

BLAST of CmoCh15G000140 vs. ExPASy Swiss-Prot
Match: Q5U378 (Tubulin-specific chaperone E OS=Danio rerio OX=7955 GN=tbce PE=2 SV=2)

HSP 1 Score: 223.4 bits (568), Expect = 6.2e-57
Identity = 179/548 (32.66%), Postives = 275/548 (50.18%), Query Frame = 0

Query: 7   LQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWD-DSNGKHDGSINGVRYFQ 66
           + L+  +G+RV   G+    GTV +VG +   +G W+GV+WD    GKHDGS +GVRYF 
Sbjct: 1   MMLDEAVGRRVCCDGER---GTVRYVGPVPPTAGVWLGVEWDHPERGKHDGSHDGVRYFT 60

Query: 67  AKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDL 126
            +    GSFVR Q  SFGV  + AL+ RY  +  +   +EM + S      +V  VG + 
Sbjct: 61  CRHPTGGSFVRPQKASFGVDYVTALKQRYEVEIEEVTAEEMKISSK-----TVVMVGFEN 120

Query: 127 IKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQL 186
           +K K S  + LT V L    VS+PG   +I +  P V+ LDL+GNLLS W+ ++ I +QL
Sbjct: 121 VKKKQS-VKNLTEVGLRRCEVSAPGPENEIRNTTPFVQSLDLSGNLLSSWEVLAAITEQL 180

Query: 187 QALVALILSNNLLSRDISGPVQL----NHIRILVLNNTGITWMQVETLEHSLPTIEELHL 246
            +L  L LS+N LS   S P  L    +H+R+L +N+  +TW QV         +EEL+L
Sbjct: 181 DSLQELHLSHNRLSIS-SAPSSLSSAFSHLRVLSINSCALTWTQVLHCAPMWQQVEELYL 240

Query: 247 MGNKIS-TVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 306
             N I+  + PE           L +L+L NN IA+   +L++  L  L+++ L++  LS
Sbjct: 241 ADNNITELLRPE------HVLQALTVLDLSNNQIAQ-ETVLEISHLPRLERLNLSSTSLS 300

Query: 307 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 366
            I           F DV        F  L+ L L  NNI +   ++ L   P L+ +   
Sbjct: 301 EI----------KFSDVPAGKKTTLFPALKELLLDDNNISEWRVVNELEKLPSLVYLSCR 360

Query: 367 ENPIADPMRG-GIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKL------- 426
            NP+    +     R ++IARL ++++++  ++   ERR +E+ Y +M  S         
Sbjct: 361 RNPLLHKEKNLETARQIMIARLGQLELLDMRQILSDERRGAELDYCKMFGSAWLRAGGHR 420

Query: 427 ---DSNPE-ETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGE 486
               +NP  + +  HPR+  L + YG  D    +    P  L + L++IT  C      E
Sbjct: 421 EAEKNNPNTDFMTEHPRYLTLIQKYGAPDEG-ELREQKPFALKNQLLTITFLC--PEDLE 480

Query: 487 KPPLTKKLPLTTTVGKLKMLCESFFKLKCMRLKL-YLQEQDSPVPIPLEDDMASLMDLGV 536
           + P+ KKLP +  V K+K L     KL  + LKL Y   + +   I +++D+  L    V
Sbjct: 481 RKPIEKKLPGSMIVQKVKGLLHRLLKLPGVELKLTYTCAKMADREIEIDNDLKPLQFYSV 518

BLAST of CmoCh15G000140 vs. ExPASy Swiss-Prot
Match: Q5U508 (Tubulin-specific chaperone E OS=Xenopus laevis OX=8355 GN=tbce PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 6.2e-57
Identity = 162/525 (30.86%), Postives = 258/525 (49.14%), Query Frame = 0

Query: 28  TVAFVGTLEGYSGTWIGVDWDDS-NGKHDGSINGVRYFQAKSERSGSFVRAQNLSFGVSL 87
           TV +VG +    G W+GV+WD+   GKH+G+  G +YF       GSF+R +  +FGV  
Sbjct: 22  TVRYVGNVPPTPGLWLGVEWDNHLRGKHNGTHEGTKYFTCSHPTGGSFIRLKKANFGVDF 81

Query: 88  LQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLSRFEELTSVSLSYMGV 147
           L AL  RY     K E++E  V+     + +V+ VG + I+++ S+  +L  VSL    V
Sbjct: 82  LAALRKRY---GLKSEQNEELVIG----KKTVELVGFESIQEEQSKLNKLKDVSLRECAV 141

Query: 148 SSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVALILSNNLL---SRDIS 207
           S+ G   QI    PN+   DL+ NL S W+ ++ I  QL+ L +L LS N L   S   S
Sbjct: 142 SNAGEKGQICHSCPNIMTADLSKNLFSSWESLAHISSQLENLTSLDLSENKLNPSSNPSS 201

Query: 208 GPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELHLMGNKISTVTPESSSPMVQGFNL 267
                 ++++L LN TG+ W ++       P +EELHL+ N IS +        V     
Sbjct: 202 LATSFCNLKVLSLNRTGMKWNEILQCASMWPALEELHLVSNDISLL-----EQPVNNLQN 261

Query: 268 LRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLSRIFYPSLDELHKLFGDVEPQGDC 327
           L +L++ NN I + +++  +  L  LKQ+ ++NN +S I +P +D  H            
Sbjct: 262 LTILDISNNKIVDGNQLHTIAFLPRLKQVIVSNNIISSISFPDVDFGHTAM--------- 321

Query: 328 FPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIAD-PMRGGIPRYVLIARLS 387
             F +L +L + GNNI +   I+ L+    L  +    NP+ D        R ++IA++ 
Sbjct: 322 --FISLTSLAVNGNNISEWCVINELHKLLHLESLNCHGNPLMDLDKNPETVRQLIIAKIE 381

Query: 388 KVQIINGSEVTPRERRDSEIRYVRMV-----------MSKLDSNPEETLRLHPRFEELKR 447
            ++ +N +E+ P ERR +E+ Y +M              + +    + L+ HPR+  L +
Sbjct: 382 NLKFLNKTEIFPTERRGAELDYRKMFGNEWLKAGGSQNEEFNKPSRDFLQDHPRYSALIK 441

Query: 448 FYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLCES 507
            YG  D    +    P  L + L+++T+ C      +K P+ KKLP + TV K+K L   
Sbjct: 442 KYGAPDEG-ELKQQQPFALKNQLLTLTIQC--PEKPDKKPIQKKLPDSMTVQKVKGLLYR 501

Query: 508 FFKLKCMRLKL-YLQEQDSPVPIPLEDDMASLMDLGVANGSNILV 536
             K+    LKL Y   +     I LE+D+  L    V NG  +LV
Sbjct: 502 LLKVPGSDLKLSYQSSKMEGKEIELENDLKPLQFYSVENGDCLLV 520

BLAST of CmoCh15G000140 vs. ExPASy Swiss-Prot
Match: Q5FVQ9 (Tubulin-specific chaperone E OS=Rattus norvegicus OX=10116 GN=Tbce PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 3.2e-53
Identity = 163/539 (30.24%), Postives = 260/539 (48.24%), Query Frame = 0

Query: 13  LGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDD-SNGKHDGSINGVRYFQAKSERS 72
           +G+RV   G+     TV F G +   +G W+GV+WD+   GKHDGS  G  YF+ +    
Sbjct: 10  IGRRVEVNGE---YATVRFCGAVPPVAGLWLGVEWDNPERGKHDGSHEGTMYFKCRHPTG 69

Query: 73  GSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLS 132
           GSFVR   ++FG   L AL+ RY      +++++   L    K+  VQ +G + I  K S
Sbjct: 70  GSFVRPNIVNFGEDFLTALKKRYVLTDGPDDDEKSCSLKVGSKQ--VQTIGFEHITKKQS 129

Query: 133 RFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVAL 192
           +   L  +SL    VS  G   +I    PN++ +DL+ NLLS W ++ +I +QL+ L AL
Sbjct: 130 QLRSLQDISLWKCAVSCAGERGRIAEACPNIRVVDLSKNLLSTWDEVILIAEQLKDLEAL 189

Query: 193 ILSNNLLSRDISGPV---QLNHIRILVLNNTGITWMQVETLEHSLPTIEELHLMGNKIST 252
            LS N L      P      + ++ LVLN TGITW +V     S P ++EL+L  N IS 
Sbjct: 190 DLSENKLQFPSDSPTLTRTFSTLKTLVLNKTGITWTEVLHCAPSWPVLQELYLKSNGISI 249

Query: 253 VTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLSRIFYPSLD 312
                S   V     LRLL+L +N   + S++  +  L  L+ + L++  LS I +P   
Sbjct: 250 -----SERPVNALQNLRLLDLSSNPSIDESQLCLIAYLPRLEHLLLSDIGLSSIHFP--- 309

Query: 313 ELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPM 372
                  D E       F  L  L +  N I + + I+ L+    L  +  + NP+    
Sbjct: 310 -------DAEIGCKTSMFPALTYLIVNDNQISEWSFINELDKLQSLQALSCARNPLT--- 369

Query: 373 RGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMV---MSKLDSNPE-------- 432
           +G     ++IA++ +++ +N  ++ P ERR +E+ Y +       K   +P+        
Sbjct: 370 KGDKAEEIIIAKIGQLKTLNRCQILPEERRGAELDYRKAFGKEWRKAGGHPDPDRNRPSA 429

Query: 433 ETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLP 492
           E L  HPR++ L   YG  ++   +    P  L + L+++ + C  ++  E+  L K+LP
Sbjct: 430 EFLSAHPRYQLLCCKYGAPEDE-ELKTQQPFMLKNQLLTLKIKC--SNQPEQQILEKQLP 489

Query: 493 LTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSP-VPIPLEDDMASLMDLGVANGSNILV 536
            + T+ K+K L     K+    L L  +    P   I LE+D+  L    V NG  +LV
Sbjct: 490 DSMTIQKVKGLLSRLLKVPVSELLLSYESSKMPGREIELENDLQPLQFYSVENGDCLLV 522

BLAST of CmoCh15G000140 vs. ExPASy Swiss-Prot
Match: Q8CIV8 (Tubulin-specific chaperone E OS=Mus musculus OX=10090 GN=Tbce PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.2e-52
Identity = 161/539 (29.87%), Postives = 260/539 (48.24%), Query Frame = 0

Query: 13  LGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDD-SNGKHDGSINGVRYFQAKSERS 72
           +G+RV   G+     TV F G +   +G W+GV+WD+   GKHDGS  G  YF+ +    
Sbjct: 10  IGRRVEVNGE---YATVRFCGAVPPVAGLWLGVEWDNPERGKHDGSHEGTMYFKCRHPTG 69

Query: 73  GSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDKLS 132
           GSFVR   ++FG   L AL+ RY  +   ++++    L    K+  VQ +G + I  K S
Sbjct: 70  GSFVRPSKVNFGDDFLTALKKRYVLEDGPDDDENSCSLKVGSKQ--VQTIGFEHITKKQS 129

Query: 133 RFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALVAL 192
           +   L  +SL    VS  G   +I    PN++ ++L+ NLLS W ++ +I +QL+ L AL
Sbjct: 130 QLRALQDISLWNCAVSHAGEQGRIAEACPNIRVVNLSKNLLSTWDEVVLIAEQLKDLEAL 189

Query: 193 ILSNNLLSRDISGPV---QLNHIRILVLNNTGITWMQVETLEHSLPTIEELHLMGNKIST 252
            LS N L      P      + ++ LVLN TGITW +V     S P +EEL+L  N IS 
Sbjct: 190 DLSENKLQFPSDSPTLTRTFSTLKTLVLNKTGITWTEVLHCAPSWPVLEELYLKSNNISI 249

Query: 253 VTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLSRIFYPSLD 312
                S   V     +RLL+L +N   + S++  +  L  L+ + L++  LS I +P   
Sbjct: 250 -----SERPVNVLQKMRLLDLSSNPSIDESQLSLIADLPRLEHLVLSDIGLSSIHFP--- 309

Query: 313 ELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPM 372
                  D E       F  L+ L +  N I + + I+ L+    L  +  + NP++   
Sbjct: 310 -------DAEIGCKTSMFPALKYLIVNDNQISEWSFINELDKLQSLQALSCTRNPLS--- 369

Query: 373 RGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMV---MSKLDSNPEET------ 432
           +      ++IA++++++ +N  ++ P ERR +E+ Y +       K   +P+        
Sbjct: 370 KADKAEEIIIAKIAQLRTLNRCQILPEERRGAELDYRKAFGNEWRKAGGHPDPDKNRPNA 429

Query: 433 --LRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLP 492
             L  HPR++ L   YG  ++   +    P  L   L+++ + C  ++  E+  L K+LP
Sbjct: 430 AFLSAHPRYQLLCCKYGAPEDE-ELKTQQPFMLKKQLLTLKIKC--SNQPERQILEKQLP 489

Query: 493 LTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSP-VPIPLEDDMASLMDLGVANGSNILV 536
            + TV K+K L     K+    L L  +    P   I LE+D+  L    V NG  +LV
Sbjct: 490 DSMTVQKVKGLLSRLLKVPVSELLLSYESSKMPGREIELENDLQPLQFYSVENGDCLLV 522

BLAST of CmoCh15G000140 vs. ExPASy TrEMBL
Match: A0A6J1FNN5 (Tubulin-folding cofactor E OS=Cucurbita moschata OX=3662 GN=LOC111445549 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 7.5e-308
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
           RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 539

BLAST of CmoCh15G000140 vs. ExPASy TrEMBL
Match: A0A6J1K072 (Tubulin-folding cofactor E OS=Cucurbita maxima OX=3661 GN=LOC111489449 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.2e-301
Identity = 527/539 (97.77%), Postives = 534/539 (99.07%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQGSIQLQLEFR GQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRPGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVRAQNLSFG+SLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGN LSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNFLSDWKDISI 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           ICDQLQALVALILSNNLLSRDISGPVQL+HIRILVLNNTGITWMQVETL+HSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLSHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGNKISTVTPESSSP+VQGFNLLRLLNLENNCIAEWSEILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPIVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLEQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
           RIFYPSLDELHKLFGDVE QGDC PFRNL+NLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVESQGDCLPFRNLQNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           TVGKLKMLCESFFKLKC+RLKLYLQEQDSPVPI LEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCVRLKLYLQEQDSPVPIQLEDDMASLMDLGVANGSNILVDEES 539

BLAST of CmoCh15G000140 vs. ExPASy TrEMBL
Match: A0A1S3BEU4 (Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.9e-269
Identity = 473/539 (87.76%), Postives = 501/539 (92.95%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQ S Q Q EFRLGQRVHFVGDPRR GTVAF+GTLEGYSGTW+GVDWDD+NGKHDGSING
Sbjct: 1   MQDSFQPQSEFRLGQRVHFVGDPRRTGTVAFIGTLEGYSGTWVGVDWDDNNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVR QNLS G+SLLQAL+LRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRVQNLSIGISLLQALDLRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSS G+P QIGSVLPN+KQLDLTGNLLSDWKDIS 
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSLGDPGQIGSVLPNLKQLDLTGNLLSDWKDIST 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           +CDQLQALVA+ILSNNLLS +ISGP+QL HIRILVLNNTGITWMQVE L+HSLP IEELH
Sbjct: 181 LCDQLQALVAIILSNNLLSCEISGPLQLKHIRILVLNNTGITWMQVEILKHSLPAIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGN IS V PESSS MV+GFNLLRLLNLENNCIAEW+EILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNNISEVKPESSS-MVEGFNLLRLLNLENNCIAEWNEILKLGQLRSLEQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
            IFYP+LDELH+LFGDVE QGDCFPF+NLR LFLGGNNIDDLASID LNSFP LIDIRLS
Sbjct: 301 HIFYPNLDELHELFGDVELQGDCFPFQNLRCLFLGGNNIDDLASIDVLNSFPNLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSK+QIINGSEVTPRERRDSEIRY+RMVMS LD N E+T 
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKIQIINGSEVTPRERRDSEIRYIRMVMSNLDGNREKTF 420

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNR SVG  GPQKL+SGLISITL CVGASIGEKPP+TKKLP  T
Sbjct: 421 RLHPRFEELKRFYGIEDNRASVGPAGPQKLSSGLISITLKCVGASIGEKPPVTKKLPPAT 480

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           +VGKLKMLCESFFKLK ++LKLYLQE+DSP+PI LEDDM SLMDLGV N SNILVDEES
Sbjct: 481 SVGKLKMLCESFFKLKSIKLKLYLQEEDSPMPILLEDDMTSLMDLGVGNESNILVDEES 538

BLAST of CmoCh15G000140 vs. ExPASy TrEMBL
Match: A0A5A7SYG1 (Tubulin-folding cofactor E OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002640 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.9e-269
Identity = 473/539 (87.76%), Postives = 501/539 (92.95%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQ S Q Q EFRLGQRVHFVGDPRR GTVAF+GTLEGYSGTW+GVDWDD+NGKHDGSING
Sbjct: 1   MQDSFQPQSEFRLGQRVHFVGDPRRTGTVAFIGTLEGYSGTWVGVDWDDNNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVR QNLS G+SLLQAL+LRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRVQNLSIGISLLQALDLRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSS G+P QIGSVLPN+KQLDLTGNLLSDWKDIS 
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSLGDPGQIGSVLPNLKQLDLTGNLLSDWKDIST 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           +CDQLQALVA+ILSNNLLS +ISGP+QL HIRILVLNNTGITWMQVE L+HSLP IEELH
Sbjct: 181 LCDQLQALVAIILSNNLLSCEISGPLQLKHIRILVLNNTGITWMQVEILKHSLPAIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGN IS V PESSS MV+GFNLLRLLNLENNCIAEW+EILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNNISEVKPESSS-MVEGFNLLRLLNLENNCIAEWNEILKLGQLRSLEQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
            IFYP+LDELH+LFGDVE QGDCFPF+NLR LFLGGNNIDDLASID LNSFP LIDIRLS
Sbjct: 301 HIFYPNLDELHELFGDVELQGDCFPFQNLRCLFLGGNNIDDLASIDVLNSFPNLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSK+QIINGSEVTPRERRDSEIRY+RMVMS LD N E+T 
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKIQIINGSEVTPRERRDSEIRYIRMVMSNLDGNREKTF 420

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNR SVG  GPQKL+SGLISITL CVGASIGEKPP+TKKLP  T
Sbjct: 421 RLHPRFEELKRFYGIEDNRASVGPAGPQKLSSGLISITLKCVGASIGEKPPVTKKLPPAT 480

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           +VGKLKMLCESFFKLK ++LKLYLQE+DSP+PI LEDDM SLMDLGV N SNILVDEES
Sbjct: 481 SVGKLKMLCESFFKLKSIKLKLYLQEEDSPMPILLEDDMTSLMDLGVGNESNILVDEES 538

BLAST of CmoCh15G000140 vs. ExPASy TrEMBL
Match: A0A1S3BEN9 (Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.9e-269
Identity = 473/539 (87.76%), Postives = 501/539 (92.95%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQ S Q Q EFRLGQRVHFVGDPRR GTVAF+GTLEGYSGTW+GVDWDD+NGKHDGSING
Sbjct: 10  MQDSFQPQSEFRLGQRVHFVGDPRRTGTVAFIGTLEGYSGTWVGVDWDDNNGKHDGSING 69

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVR QNLS G+SLLQAL+LRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 70  VRYFQAKSERSGSFVRVQNLSIGISLLQALDLRYRGDSTKEEEDEMYVLSASDKRVSVQF 129

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSS G+P QIGSVLPN+KQLDLTGNLLSDWKDIS 
Sbjct: 130 VGKDLIKDKLSRFEELTSVSLSYMGVSSLGDPGQIGSVLPNLKQLDLTGNLLSDWKDIST 189

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           +CDQLQALVA+ILSNNLLS +ISGP+QL HIRILVLNNTGITWMQVE L+HSLP IEELH
Sbjct: 190 LCDQLQALVAIILSNNLLSCEISGPLQLKHIRILVLNNTGITWMQVEILKHSLPAIEELH 249

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGN IS V PESSS MV+GFNLLRLLNLENNCIAEW+EILKLGQLRSL+QIQLNNNKLS
Sbjct: 250 LMGNNISEVKPESSS-MVEGFNLLRLLNLENNCIAEWNEILKLGQLRSLEQIQLNNNKLS 309

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
            IFYP+LDELH+LFGDVE QGDCFPF+NLR LFLGGNNIDDLASID LNSFP LIDIRLS
Sbjct: 310 HIFYPNLDELHELFGDVELQGDCFPFQNLRCLFLGGNNIDDLASIDVLNSFPNLIDIRLS 369

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSK+QIINGSEVTPRERRDSEIRY+RMVMS LD N E+T 
Sbjct: 370 ENPIADPMRGGIPRYVLIARLSKIQIINGSEVTPRERRDSEIRYIRMVMSNLDGNREKTF 429

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNR SVG  GPQKL+SGLISITL CVGASIGEKPP+TKKLP  T
Sbjct: 430 RLHPRFEELKRFYGIEDNRASVGPAGPQKLSSGLISITLKCVGASIGEKPPVTKKLPPAT 489

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           +VGKLKMLCESFFKLK ++LKLYLQE+DSP+PI LEDDM SLMDLGV N SNILVDEES
Sbjct: 490 SVGKLKMLCESFFKLKSIKLKLYLQEEDSPMPILLEDDMTSLMDLGVGNESNILVDEES 547

BLAST of CmoCh15G000140 vs. NCBI nr
Match: XP_022939765.1 (tubulin-folding cofactor E [Cucurbita moschata])

HSP 1 Score: 1065.4 bits (2754), Expect = 1.6e-307
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
           RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 539

BLAST of CmoCh15G000140 vs. NCBI nr
Match: KAG7015887.1 (Tubulin-folding cofactor E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1057.0 bits (2732), Expect = 5.5e-305
Identity = 538/546 (98.53%), Postives = 539/546 (98.72%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETL+HSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
           RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIR-------YVRMVMSKLD 420
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIR       YVRMVMSKLD
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRDFNSSCSYVRMVMSKLD 420

Query: 421 SNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLT 480
           SNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLT
Sbjct: 421 SNPEETLRLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLT 480

Query: 481 KKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNI 540
           KKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNI
Sbjct: 481 KKLPLTTTVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNI 540

BLAST of CmoCh15G000140 vs. NCBI nr
Match: XP_023551442.1 (tubulin-folding cofactor E [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.0e-303
Identity = 531/539 (98.52%), Postives = 534/539 (99.07%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQGSIQLQLEFRLGQR HFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 14  MQGSIQLQLEFRLGQRAHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 73

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVRAQNLSFG+SLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 74  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 133

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 134 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 193

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETL+HSLPTIEELH
Sbjct: 194 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 253

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCI EWSEILKLGQLRSL+QIQLNNNKLS
Sbjct: 254 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 313

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
           RIFYPS DELHKLFGDVE QGDC PFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 314 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 373

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 374 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 433

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 434 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 493

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Sbjct: 494 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 552

BLAST of CmoCh15G000140 vs. NCBI nr
Match: KAG6578311.1 (Tubulin-folding cofactor E, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.5e-302
Identity = 530/539 (98.33%), Postives = 534/539 (99.07%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQGSIQLQLEFRL QRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRLDQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVRAQNLSFG+SLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETL+HSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGNK+STVTPESSSPMVQGFNLLRLLNLENNCI EWSEILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNKMSTVTPESSSPMVQGFNLLRLLNLENNCIVEWSEILKLGQLRSLEQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
           RIFYPS DELHKLFGDVE QGDC PFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSFDELHKLFGDVESQGDCSPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 539

BLAST of CmoCh15G000140 vs. NCBI nr
Match: XP_022993434.1 (tubulin-folding cofactor E [Cucurbita maxima])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.1e-300
Identity = 527/539 (97.77%), Postives = 534/539 (99.07%), Query Frame = 0

Query: 1   MQGSIQLQLEFRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60
           MQGSIQLQLEFR GQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING
Sbjct: 1   MQGSIQLQLEFRPGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWDDSNGKHDGSING 60

Query: 61  VRYFQAKSERSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120
           VRYFQAKSERSGSFVRAQNLSFG+SLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF
Sbjct: 61  VRYFQAKSERSGSFVRAQNLSFGISLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQF 120

Query: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISI 180
           VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGN LSDWKDISI
Sbjct: 121 VGKDLIKDKLSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNFLSDWKDISI 180

Query: 181 ICDQLQALVALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELH 240
           ICDQLQALVALILSNNLLSRDISGPVQL+HIRILVLNNTGITWMQVETL+HSLPTIEELH
Sbjct: 181 ICDQLQALVALILSNNLLSRDISGPVQLSHIRILVLNNTGITWMQVETLKHSLPTIEELH 240

Query: 241 LMGNKISTVTPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLS 300
           LMGNKISTVTPESSSP+VQGFNLLRLLNLENNCIAEWSEILKLGQLRSL+QIQLNNNKLS
Sbjct: 241 LMGNKISTVTPESSSPIVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLEQIQLNNNKLS 300

Query: 301 RIFYPSLDELHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360
           RIFYPSLDELHKLFGDVE QGDC PFRNL+NLFLGGNNIDDLASIDFLNSFPQLIDIRLS
Sbjct: 301 RIFYPSLDELHKLFGDVESQGDCLPFRNLQNLFLGGNNIDDLASIDFLNSFPQLIDIRLS 360

Query: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420
           ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL
Sbjct: 361 ENPIADPMRGGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETL 420

Query: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480
           RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT
Sbjct: 421 RLHPRFEELKRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTT 480

Query: 481 TVGKLKMLCESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           TVGKLKMLCESFFKLKC+RLKLYLQEQDSPVPI LEDDMASLMDLGVANGSNILVDEES
Sbjct: 481 TVGKLKMLCESFFKLKCVRLKLYLQEQDSPVPIQLEDDMASLMDLGVANGSNILVDEES 539

BLAST of CmoCh15G000140 vs. TAIR 10
Match: AT1G71440.1 (tubulin folding cofactor E / Pfifferling (PFI) )

HSP 1 Score: 649.4 bits (1674), Expect = 2.5e-186
Identity = 335/530 (63.21%), Postives = 412/530 (77.74%), Query Frame = 0

Query: 11  FRLGQRVHFVGDPRRVGTVAFVGTLEGYSGTWIGVDWD-DSNGKHDGSINGVRYFQAKSE 70
           F +GQRVH + D RRVGTV +VG +EGYSGTWIGVDWD D +GKH+GS+NGV YF  +S+
Sbjct: 10  FIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYFNGRSQ 69

Query: 71  RSGSFVRAQNLSFGVSLLQALELRYRGDSTKEEEDEMYVLSASDKRVSVQFVGKDLIKDK 130
            S SFVR+QNLS G++LLQALELRYR  STK+EEDEMYVLSA ++RVS+Q +G D I+DK
Sbjct: 70  SSASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGGDKIQDK 129

Query: 131 LSRFEELTSVSLSYMGVSSPGNPCQIGSVLPNVKQLDLTGNLLSDWKDISIICDQLQALV 190
           LSRFEELTS SLSY+GVSS G    +GS+LPN+K LDLTGNL+SDW++I  +C+QL AL 
Sbjct: 130 LSRFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCEQLPALT 189

Query: 191 ALILSNNLLSRDISGPVQLNHIRILVLNNTGITWMQVETLEHSLPTIEELHLMGNKISTV 250
            L LS N LS DI    QL +IR+LVLNN+G++W QVE L  SLP IEELHLMGN IST+
Sbjct: 190 TLNLSCNSLSSDIKSLPQLKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMGNMISTI 249

Query: 251 TPESSSPMVQGFNLLRLLNLENNCIAEWSEILKLGQLRSLKQIQLNNNKLSRIFYPSLDE 310
           T  SSS   Q FN LRLLNL++NCI++WSE+LKL QL  L+Q+ LN NKLSRIF      
Sbjct: 250 TSTSSSD-DQAFNSLRLLNLDDNCISDWSEVLKLSQLPCLEQLYLNKNKLSRIF------ 309

Query: 311 LHKLFGDVEPQGDCFPFRNLRNLFLGGNNIDDLASIDFLNSFPQLIDIRLSENPIADPMR 370
              + G    +    PF +L  L LG NNI DLAS+D LN FPQL+DIRLSENPI+DP+R
Sbjct: 310 -QSVNGTESSEKGSDPFPSLSCLLLGANNIGDLASVDALNGFPQLVDIRLSENPISDPVR 369

Query: 371 GGIPRYVLIARLSKVQIINGSEVTPRERRDSEIRYVRMVMSKLDSNPEETLRLHPRFEEL 430
           GG+PR+VL+ARL+KVQ++NGSEV  RE++DSEIRYVRMVMSKL+    E   LHPRF EL
Sbjct: 370 GGVPRFVLVARLTKVQVLNGSEVRAREKKDSEIRYVRMVMSKLNDKSGEIELLHPRFYEL 429

Query: 431 KRFYGIEDNRVSVGAGGPQKLASGLISITLNCVGASIGEKPPLTKKLPLTTTVGKLKMLC 490
           K+ +GIED R S    GP+ +ASGLISITL CVG S+GEKP LTKKLP + TVGKLK+L 
Sbjct: 430 KKLHGIEDERASAENSGPKNIASGLISITLKCVGPSMGEKPHLTKKLPGSITVGKLKILS 489

Query: 491 ESFFKLKCMRLKLYLQEQDSPVPIPLEDDMASLMDLGVANGSNILVDEES 540
           E+FFKLK ++ +L+LQE+ SP P  L+D+ A+L+D+G+ +GS +LVDEES
Sbjct: 490 ENFFKLKSIKPRLFLQEEGSPFPTALDDETATLLDVGICDGSTLLVDEES 531

BLAST of CmoCh15G000140 vs. TAIR 10
Match: AT3G10220.1 (tubulin folding cofactor B )

HSP 1 Score: 44.7 bits (104), Expect = 2.8e-04
Identity = 24/73 (32.88%), Postives = 36/73 (49.32%), Query Frame = 0

Query: 12  RLGQRVHFVGDPRRVGTVAFVGTLEGYS-GTWIGVDWDDSNGKHDGSINGVRYFQAKSER 71
           ++G R       +R G V +VG  E    G W+G+ +D+  GKHDG + G R+F+     
Sbjct: 160 KVGDRCQVEPGEKR-GMVKYVGRAESLGPGYWVGIQYDEPLGKHDGMVKGTRFFEC-PRL 219

Query: 72  SGSFVRAQNLSFG 84
            G  VR   +  G
Sbjct: 220 QGGMVRPDKVKVG 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GRL73.5e-18563.21Tubulin-folding cofactor E OS=Arabidopsis thaliana OX=3702 GN=TFCE PE=2 SV=1[more]
Q5U3786.2e-5732.66Tubulin-specific chaperone E OS=Danio rerio OX=7955 GN=tbce PE=2 SV=2[more]
Q5U5086.2e-5730.86Tubulin-specific chaperone E OS=Xenopus laevis OX=8355 GN=tbce PE=2 SV=1[more]
Q5FVQ93.2e-5330.24Tubulin-specific chaperone E OS=Rattus norvegicus OX=10116 GN=Tbce PE=2 SV=1[more]
Q8CIV81.2e-5229.87Tubulin-specific chaperone E OS=Mus musculus OX=10090 GN=Tbce PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FNN57.5e-308100.00Tubulin-folding cofactor E OS=Cucurbita moschata OX=3662 GN=LOC111445549 PE=3 SV... [more]
A0A6J1K0725.2e-30197.77Tubulin-folding cofactor E OS=Cucurbita maxima OX=3661 GN=LOC111489449 PE=3 SV=1[more]
A0A1S3BEU46.9e-26987.76Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1[more]
A0A5A7SYG16.9e-26987.76Tubulin-folding cofactor E OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BEN96.9e-26987.76Tubulin-folding cofactor E OS=Cucumis melo OX=3656 GN=LOC103488852 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022939765.11.6e-307100.00tubulin-folding cofactor E [Cucurbita moschata][more]
KAG7015887.15.5e-30598.53Tubulin-folding cofactor E [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023551442.13.0e-30398.52tubulin-folding cofactor E [Cucurbita pepo subsp. pepo][more]
KAG6578311.11.5e-30298.33Tubulin-folding cofactor E, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022993434.11.1e-30097.77tubulin-folding cofactor E [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G71440.12.5e-18663.21tubulin folding cofactor E / Pfifferling (PFI) [more]
AT3G10220.12.8e-0432.88tubulin folding cofactor B [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000938CAP Gly-rich domainSMARTSM01052CAP_GLY_2coord: 13..81
e-value: 4.5E-28
score: 109.3
IPR000938CAP Gly-rich domainPFAMPF01302CAP_GLYcoord: 13..79
e-value: 4.3E-20
score: 71.4
IPR000938CAP Gly-rich domainPROSITEPS00845CAP_GLY_1coord: 33..64
IPR000938CAP Gly-rich domainPROSITEPS50245CAP_GLY_2coord: 33..76
score: 16.923649
IPR003591Leucine-rich repeat, typical subtypeSMARTSM00369LRR_typ_2coord: 208..231
e-value: 320.0
score: 0.9
coord: 159..182
e-value: 180.0
score: 3.0
coord: 286..305
e-value: 90.0
score: 5.4
coord: 233..256
e-value: 51.0
score: 7.5
coord: 326..349
e-value: 38.0
score: 8.5
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 202..312
e-value: 2.6E-12
score: 48.1
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 313..413
e-value: 1.7E-12
score: 48.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 89..201
e-value: 8.5E-10
score: 40.1
NoneNo IPR availableGENE3D3.10.20.90coord: 446..539
e-value: 3.8E-18
score: 67.3
NoneNo IPR availablePANTHERPTHR15140:SF6TUBULIN-SPECIFIC CHAPERONE Ecoord: 11..537
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 127..388
IPR036859CAP Gly-rich domain superfamilyGENE3D2.30.30.190coord: 5..88
e-value: 3.8E-22
score: 79.7
IPR036859CAP Gly-rich domain superfamilySUPERFAMILY74924Cap-Gly domaincoord: 7..147
IPR044994Tubulin-specific chaperone EPANTHERPTHR15140TUBULIN-SPECIFIC CHAPERONE Ecoord: 11..537
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 263..284
score: 7.21911
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 328..349
score: 8.674542
IPR044079TBCE, ubiquitin-like (Ubl) domainCDDcd17044Ubl_TBCEcoord: 454..536
e-value: 1.39424E-22
score: 89.9467
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 450..536

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G000140.1CmoCh15G000140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
biological_process GO:0007023 post-chaperonin tubulin folding pathway
biological_process GO:0007021 tubulin complex assembly
biological_process GO:0006457 protein folding
cellular_component GO:0005737 cytoplasm
molecular_function GO:0043014 alpha-tubulin binding
molecular_function GO:0005515 protein binding