CmoCh14G020620 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh14G020620
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionCRS2-associated factor 1, mitochondrial
LocationCmo_Chr14: 15052207 .. 15054744 (-)
RNA-Seq ExpressionCmoCh14G020620
SyntenyCmoCh14G020620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAACCGATTTCGCCGATAAACCCTAAACCATTTCCGGTCGCGCGACATTGTTCATCCATGCGCAATCTACCTCATGTTCGTTATCAGACTCTTCCGCCAGAATCCAATTCCGTTTGCTCATCCTCATACACCTTCCAATCGATGTTTCTGCTACTCCAAACTCCGGGATCACTACGCTTTTGAATCTCCACCTTCTCTCTCCCCTAAACCCCCACCGCCGGAGCTCAAACCGCGGCAAAACAAACCACTGAAACCGCGGTACAAACCACCATCGTCGCTCGATCTCGCTGACAAGAAGCCAAAGCATTCAGACTTGCCATTTGATTTCCAGTACAGTTACACGGAGACTAGCCTCTCGGTGAGGCCTATCGGGCTTCGAGAACCGAAGTTTTCACCGTTTGGGCCTGGGCGGCTTGACCGGAAATGGACTGGGGTGTGTGCCCCGGCGGTGGACCCGAAGGTGAGCTCTCTAGAGAGCACGGAAGATCCAAAACTGGAAGAGAAGAGAAGGGTCATGTGGGAGAATATTCAAGGGGAGCCACTGACAGGAGCCGAGAGAAAGACTTTGGTGGAAACTTGTCAAAAGAAGAAAACAAAGAGGCAGATTAATTTGGGTATGTGATACCAAGTTCTATTAATCTATTGTTTGCACATATAAAATTGTTGATCTTGGACGCTGTTTTCTCATCATTTCTAATTGATAGATGCTTCATGGATATTGAGATAATCTTATTTTCTGTGCTTAAATTACTGAGGCATCTGATAATACGATCTCTATATTGAATGGCTCCGTTGGGATAATTGCATTTTCTTCTAACTACATAACTTCTATGCGTTCGTAGGAAGAGATGGTTTAACTCACAATATGTTAAATGATATCTATAATCACTGGAAACATGCTGAGGCAGTGAGGATCAAATGTTTAGGTGTTCCCACTGTTGATATGAAAAATGTGTGCACGCAGCTTGAGGTATGGCTTTCATTTCTTTGGGTGGAGTTTTCTTGTTATAGGCAAATGTTTTAGTTCTTCATTTCTTAACCTATTGATATTGTTCCACAAATTACATTCAACATATTGAAAATGTATTTTGAGTCACACCCACCTGTTCGTGTACGTTTAATTCCGAGGTTTGTCCATGCCTGCAGGAGAAAACCTTTGGAAAGATCATCCATAGGCATGGTGGTTTTCTTGTTCTATACAGAGGGAGGAATTATAATCCCAAGAAAAGGCCATTCATTCCCTTAATGTTATGGAGACCCCATGAACCTGTATATCCCAGGCTGATTAAAACTACAATTGATGGACTGAGCATTAATGAAACAAAAGAAATGAGAAAGAAAGGACTAGCTGTTCCTGCTTTGACAAGACTTGGTATGTGTTTGAGAACTTTTTTTTAAAGCTTGTTTCGAGTAATATTACCGTCTCATCATAACGTCATTGACTAATGCATCATGCCTGTGGATTTTAGCAAAAAATGGTTACTATGGTAGTTTGGTACCCATGGTTCGAGATGCTTTTCTCTCCTGCGAGTTGGTTCGGATTGATTGTCAAGGCCTTGAGAAGAGTGATTACAAGAAAATTGGCTGCAAGCTTCGGGTAATGGTCGTTTCCCTCATGCCTTTATGTAACTATTCTTAAATTCTTAAATTCTTAAATTCTTAAATCCTTAAATTCTTAAATTCTTAAATCATAGAAAGAAAGGAAGAAGGACCCTTACCATGCCATGAGTGTTGTTCTTCATGTGTACCTAGATTTTTGTTTCGTTGCATCATGCATTGGCGTTAAAAATCGGTTCTACTTGACTAAGTCCCGTGCTCATCTCCTAAGGAGGAGGGAGTTGTTTATTGTGCCAAAATTTGAATTCTTTTCATTTTTTATCAACTTTCCTTCTTAAATCAGGACCTTGTGCCTTGTATCTTGGTGACGTTTGAGAAGGAGCAGATTGTAGTGTGGAGAGGCAAGGATTACCAGCCTTCAGAATCTGAATGTTTTCTTACAGTCAGAGAGTCATTTGATGATGTTGATGGCAATACGAATTGTGTCGACAATGACGACGACAATCAAAATTTTGACGATGAATGCTCCGATTCGGGCTTTCAATCTGGTAATAACGAGTAGTCCCTTGGTTACTTCACATCCTATGCGAATCATTGTACCAAAACACCATGATCTTTTGAGCTTGTGATTTTGTTTAAGGCTACATATTAATATTGCAGCTGCAAAGCAAATTCCCAAAGACCGATGATTCGAATGCACTATGCTGTAGATTGACCCATATTGTTTGTTCATATCATTTATTGCCTCATTCTTCTGTTCTCCCATGAGGGAGTCAAAATTCTGCAGGGCATTCTTTTTTCGGGTTCAACAATACCGTAGAGCGTAGAGGTTTGAACTTTTAACTTTCTTTTTGACAATGGTGTATACTTTAGCTAGCGGAACTATGTTCATGTTGAGTTGTTCCACGCCTATATTGGCTTATCAAATTTTTGTTATCGCAACCCACGAGTCTTAACTTCCCTCTAACGACTAATTTTCT

mRNA sequence

TAAACCGATTTCGCCGATAAACCCTAAACCATTTCCGGTCGCGCGACATTGTTCATCCATGCGCAATCTACCTCATGTTCGTTATCAGACTCTTCCGCCAGAATCCAATTCCGTTTGCTCATCCTCATACACCTTCCAATCGATGTTTCTGCTACTCCAAACTCCGGGATCACTACGCTTTTGAATCTCCACCTTCTCTCTCCCCTAAACCCCCACCGCCGGAGCTCAAACCGCGGCAAAACAAACCACTGAAACCGCGGTACAAACCACCATCGTCGCTCGATCTCGCTGACAAGAAGCCAAAGCATTCAGACTTGCCATTTGATTTCCAGTACAGTTACACGGAGACTAGCCTCTCGGTGAGGCCTATCGGGCTTCGAGAACCGAAGTTTTCACCGTTTGGGCCTGGGCGGCTTGACCGGAAATGGACTGGGGTGTGTGCCCCGGCGGTGGACCCGAAGGTGAGCTCTCTAGAGAGCACGGAAGATCCAAAACTGGAAGAGAAGAGAAGGGTCATGTGGGAGAATATTCAAGGGGAGCCACTGACAGGAGCCGAGAGAAAGACTTTGGTGGAAACTTGTCAAAAGAAGAAAACAAAGAGGCAGATTAATTTGGGAAGAGATGGTTTAACTCACAATATGTTAAATGATATCTATAATCACTGGAAACATGCTGAGGCAGTGAGGATCAAATGTTTAGGTGTTCCCACTGTTGATATGAAAAATGTGTGCACGCAGCTTGAGGAGAAAACCTTTGGAAAGATCATCCATAGGCATGGTGGTTTTCTTGTTCTATACAGAGGGAGGAATTATAATCCCAAGAAAAGGCCATTCATTCCCTTAATGTTATGGAGACCCCATGAACCTGTATATCCCAGGCTGATTAAAACTACAATTGATGGACTGAGCATTAATGAAACAAAAGAAATGAGAAAGAAAGGACTAGCTGTTCCTGCTTTGACAAGACTTGCAAAAAATGGTTACTATGGTAGTTTGGTACCCATGGTTCGAGATGCTTTTCTCTCCTGCGAGTTGGTTCGGATTGATTGTCAAGGCCTTGAGAAGAGTGATTACAAGAAAATTGGCTGCAAGCTTCGGGACCTTGTGCCTTGTATCTTGGTGACGTTTGAGAAGGAGCAGATTGTAGTGTGGAGAGGCAAGGATTACCAGCCTTCAGAATCTGAATGTTTTCTTACAGTCAGAGAGTCATTTGATGATGTTGATGGCAATACGAATTGTGTCGACAATGACGACGACAATCAAAATTTTGACGATGAATGCTCCGATTCGGGCTTTCAATCTGGTAATAACGAGTAGTCCCTTGGTTACTTCACATCCTATGCGAATCATTGTACCAAAACACCATGATCTTTTGAGCTTGTGATTTTGTTTAAGGCTACATATTAATATTGCAGCTGCAAAGCAAATTCCCAAAGACCGATGATTCGAATGCACTATGCTGTAGATTGACCCATATTGTTTGTTCATATCATTTATTGCCTCATTCTTCTGTTCTCCCATGAGGGAGTCAAAATTCTGCAGGGCATTCTTTTTTCGGGTTCAACAATACCGTAGAGCGTAGAGGTTTGAACTTTTAACTTTCTTTTTGACAATGGTGTATACTTTAGCTAGCGGAACTATGTTCATGTTGAGTTGTTCCACGCCTATATTGGCTTATCAAATTTTTGTTATCGCAACCCACGAGTCTTAACTTCCCTCTAACGACTAATTTTCT

Coding sequence (CDS)

ATGTTCGTTATCAGACTCTTCCGCCAGAATCCAATTCCGTTTGCTCATCCTCATACACCTTCCAATCGATGTTTCTGCTACTCCAAACTCCGGGATCACTACGCTTTTGAATCTCCACCTTCTCTCTCCCCTAAACCCCCACCGCCGGAGCTCAAACCGCGGCAAAACAAACCACTGAAACCGCGGTACAAACCACCATCGTCGCTCGATCTCGCTGACAAGAAGCCAAAGCATTCAGACTTGCCATTTGATTTCCAGTACAGTTACACGGAGACTAGCCTCTCGGTGAGGCCTATCGGGCTTCGAGAACCGAAGTTTTCACCGTTTGGGCCTGGGCGGCTTGACCGGAAATGGACTGGGGTGTGTGCCCCGGCGGTGGACCCGAAGGTGAGCTCTCTAGAGAGCACGGAAGATCCAAAACTGGAAGAGAAGAGAAGGGTCATGTGGGAGAATATTCAAGGGGAGCCACTGACAGGAGCCGAGAGAAAGACTTTGGTGGAAACTTGTCAAAAGAAGAAAACAAAGAGGCAGATTAATTTGGGAAGAGATGGTTTAACTCACAATATGTTAAATGATATCTATAATCACTGGAAACATGCTGAGGCAGTGAGGATCAAATGTTTAGGTGTTCCCACTGTTGATATGAAAAATGTGTGCACGCAGCTTGAGGAGAAAACCTTTGGAAAGATCATCCATAGGCATGGTGGTTTTCTTGTTCTATACAGAGGGAGGAATTATAATCCCAAGAAAAGGCCATTCATTCCCTTAATGTTATGGAGACCCCATGAACCTGTATATCCCAGGCTGATTAAAACTACAATTGATGGACTGAGCATTAATGAAACAAAAGAAATGAGAAAGAAAGGACTAGCTGTTCCTGCTTTGACAAGACTTGCAAAAAATGGTTACTATGGTAGTTTGGTACCCATGGTTCGAGATGCTTTTCTCTCCTGCGAGTTGGTTCGGATTGATTGTCAAGGCCTTGAGAAGAGTGATTACAAGAAAATTGGCTGCAAGCTTCGGGACCTTGTGCCTTGTATCTTGGTGACGTTTGAGAAGGAGCAGATTGTAGTGTGGAGAGGCAAGGATTACCAGCCTTCAGAATCTGAATGTTTTCTTACAGTCAGAGAGTCATTTGATGATGTTGATGGCAATACGAATTGTGTCGACAATGACGACGACAATCAAAATTTTGACGATGAATGCTCCGATTCGGGCTTTCAATCTGGTAATAACGAGTAG

Protein sequence

MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLKPRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTGVCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWRGKDYQPSESECFLTVRESFDDVDGNTNCVDNDDDNQNFDDECSDSGFQSGNNE
Homology
BLAST of CmoCh14G020620 vs. ExPASy Swiss-Prot
Match: Q8VYD9 (CRS2-associated factor 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g31010 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 3.6e-150
Identity = 274/407 (67.32%), Postives = 316/407 (77.64%), Query Frame = 0

Query: 1   MFVIRLFRQNPIPFA------HPHTPSNRCFCYSKLRDHYAFESPPSLS---PKPPPPEL 60
           MF+IRL R NP  F       H  T S+     S+LRD Y F+SPP LS    + P    
Sbjct: 1   MFLIRLSRHNPSSFTLLTRRLHDQTISS-----SRLRDLYNFQSPPPLSSSASENPDFNQ 60

Query: 61  KPRQNKPLKPRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGP 120
           K    K  KP+Y+PPSSL+    K  HSDLPFDF++SYTE+  +VRPIGLREPK+SPFGP
Sbjct: 61  KNNNKKKPKPQYRPPSSLE--GVKTVHSDLPFDFRFSYTESCSNVRPIGLREPKYSPFGP 120

Query: 121 GRLDRKWTGVCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQK 180
            RLDR+WTGVCAPAV+PKV S++  EDPKLEEKRR + E IQG  LT AERK LVE CQ+
Sbjct: 121 DRLDREWTGVCAPAVNPKVESVDGVEDPKLEEKRRKVREKIQGASLTEAERKFLVELCQR 180

Query: 181 KKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKII 240
            KTKRQ+NLGRDGLTHNMLND+YNHWKHAEAVR+KCLGVPT+DMKNV   LE+KTFG+++
Sbjct: 181 NKTKRQVNLGRDGLTHNMLNDVYNHWKHAEAVRVKCLGVPTLDMKNVIFHLEDKTFGQVV 240

Query: 241 HRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLA 300
            +H G LVLYRGRNY+PKKRP IPLMLW+PHEPVYPRLIKTTIDGLSI+ETK MRKKGLA
Sbjct: 241 SKHSGTLVLYRGRNYDPKKRPKIPLMLWKPHEPVYPRLIKTTIDGLSIDETKAMRKKGLA 300

Query: 301 VPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTF 360
           VPALT+LAKNGYYGSLVPMVRDAFL  ELVRIDC GLE+ DYKKIG KLRDLVPCILVTF
Sbjct: 301 VPALTKLAKNGYYGSLVPMVRDAFLVSELVRIDCLGLERKDYKKIGAKLRDLVPCILVTF 360

Query: 361 EKEQIVVWRGKDYQPSESE---CFLTVRESFDDVDGNTNCVDNDDDN 396
           +KEQ+V+WRGKDY+P + +        RES  D D + +C     D+
Sbjct: 361 DKEQVVIWRGKDYKPPKEDDEYSSFIHRESSIDSDVDLSCSRGAQDS 400

BLAST of CmoCh14G020620 vs. ExPASy Swiss-Prot
Match: Q6Z4U2 (CRS2-associated factor 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0174900 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 3.8e-131
Identity = 247/403 (61.29%), Postives = 294/403 (72.95%), Query Frame = 0

Query: 17  PHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLKPRYKPPSSLDLADKKP 76
           P  PS R    S L D Y F  P SL+P     +   ++ +P KP Y+PPSSLD   +  
Sbjct: 14  PRRPSVRRL--SGLLDRYGFVPPASLTPHSASDDGGAKKRRPKKPPYRPPSSLDRGGRPA 73

Query: 77  KHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTGVCAPAVDPKVSSLEST 136
             SDLPFDF++SYTE+S   +PIGLREPK+SPFGPGRLDR WTG+CAPAVD  +    + 
Sbjct: 74  ARSDLPFDFRFSYTESSPGDKPIGLREPKYSPFGPGRLDRPWTGLCAPAVDTTLRDAHA- 133

Query: 137 EDP------KLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINLGRDGLTHNML 196
           +DP      +LEE RR   E + GEPLT AER  LV  CQK +TK+QINLGRDGLTHNML
Sbjct: 134 DDPAPAAERELEEARRRERERVLGEPLTPAERAFLVSKCQKSRTKKQINLGRDGLTHNML 193

Query: 197 NDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVLYRGRNYNPKK 256
           NDI+NHWK+ EAVR+KCLGVPTVDM+NVC QLE+KT G IIHRHGG L+LYRGR+YNPKK
Sbjct: 194 NDIHNHWKNDEAVRVKCLGVPTVDMQNVCHQLEDKTGGLIIHRHGGQLILYRGRHYNPKK 253

Query: 257 RPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAKNGYYGSLVPM 316
           RP IPLMLW+P EPVYPRLIKTTI+GL++ ETKEMRKKGL VP LT+LAKNGYY SLVPM
Sbjct: 254 RPVIPLMLWKPAEPVYPRLIKTTIEGLTVEETKEMRKKGLYVPVLTKLAKNGYYASLVPM 313

Query: 317 VRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWRGKDYQPSESE 376
           VRDAFL+ ELVRID +GL KSDY+KIG KLRDLVPCI+V+F+KEQI+VWRGKDY  +  +
Sbjct: 314 VRDAFLTDELVRIDSKGLPKSDYRKIGVKLRDLVPCIIVSFDKEQIIVWRGKDYNGTIQD 373

Query: 377 CFLTVRESFDDVDGNTNCVDNDDDNQNFDDECSDSGFQSGNNE 414
                  S  + +      +N D  Q   D  SD   Q  +++
Sbjct: 374 NTQKTSVSVLEEESAGAESENGDQEQASSDWASDECSQLSSSD 413

BLAST of CmoCh14G020620 vs. ExPASy Swiss-Prot
Match: Q9FFU1 (CRS2-associated factor 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g54890 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 5.9e-92
Identity = 176/334 (52.69%), Postives = 226/334 (67.66%), Query Frame = 0

Query: 32  DHYAFESPPSLSPKPPPPELKPRQNKPLKPRYKPPSSLDLAD--KKPKHSDLPFDFQYSY 91
           D Y     P   P  PP E K ++ K      K   S +L +  K P  SDLPFDF+YSY
Sbjct: 32  DDYDPPFSPLSKPTKPPKEKKKQKTK------KQDQSSELVNDLKIPVISDLPFDFRYSY 91

Query: 92  TETSLSVRPIGLREPK-FSPFGPGRLDRKWTGVCAPAVDPKVSSLESTEDPKLEEKRRVM 151
           +ET+  + PIG REPK FSPFGPGRLDRKWTG  A      ++S E  +   +EE+ RV+
Sbjct: 92  SETNPEIEPIGFREPKRFSPFGPGRLDRKWTGTTA------LASPEIDQSQWVEERARVL 151

Query: 152 WENIQGEPLTGAERKTLVETCQKKKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCL 211
                GE LT  E   L+E  +     RQINLG+ G+THNM++DI+NHWK AEAVRIKCL
Sbjct: 152 -----GETLTEDEVTELIERYRHSDCTRQINLGKGGVTHNMIDDIHNHWKKAEAVRIKCL 211

Query: 212 GVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPR 271
           GVPT+DM N+C  LEEK+ GKI++R+   LVLYRGRNY+PK RP IPLMLW+PH P+YPR
Sbjct: 212 GVPTLDMDNICFHLEEKSGGKIVYRNINILVLYRGRNYDPKSRPIIPLMLWKPHPPIYPR 271

Query: 272 LIKTTIDGLSINETKEMRKKGLAVPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGL 331
           L+K   DGL   ETKEMR +GL  PAL +L +NG Y ++V  VR+ F + E+VR+DC  +
Sbjct: 272 LVKNVADGLEFEETKEMRNRGLHSPALMKLTRNGVYVNVVGRVREEFETEEIVRLDCTHV 331

Query: 332 EKSDYKKIGCKLRDLVPCILVTFEKEQIVVWRGK 363
             SD K+IG KL+++VPC+ + F+ EQI++WRGK
Sbjct: 332 GMSDCKRIGVKLKEMVPCVPILFKDEQIILWRGK 348

BLAST of CmoCh14G020620 vs. ExPASy Swiss-Prot
Match: Q0J7J7 (CRS2-associated factor 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0188000 PE=2 SV=2)

HSP 1 Score: 325.9 bits (834), Expect = 6.8e-88
Identity = 167/331 (50.45%), Postives = 216/331 (65.26%), Query Frame = 0

Query: 38  SPPSLSPKPPPP-----ELKPRQNKPLKPRYKPPSSLDLADKKPKHSDLPFDFQYSYTET 97
           +PP   P   PP     E  PR     K + +      +   +P  SDLPFDF+YSY+ET
Sbjct: 31  APPFSDPDDDPPFTRLAERPPRAPSKKKKKEEEDQGGRIRPPEPASSDLPFDFRYSYSET 90

Query: 98  SLSVRPIGLREP-KFSPFGPGRLDRKWTGVCAPAVDPKVSSLESTEDPKLEEKRRVMWEN 157
             + RPIG REP +FSPFGPGRLDR W GV A A   + +   +T             E 
Sbjct: 91  DPAWRPIGFREPTRFSPFGPGRLDRPWDGVAAAAARGEGAGAAATSR-----------EE 150

Query: 158 IQGEPLTGAERKTLVETCQKKKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVP 217
           + GEPL   E   LVE  +     RQINLG+ G+THNM++DI+NHWK AEAVRIKCLGVP
Sbjct: 151 VLGEPLAEEEVAQLVERYRHSDCSRQINLGKGGVTHNMIDDIHNHWKRAEAVRIKCLGVP 210

Query: 218 TVDMKNVCTQLEEKTFGKIIHRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIK 277
           T+DM N+C  LE+KT GK+I+R+   L+LYRGRNY+PK+RP IPLMLW+P  P+YPRL++
Sbjct: 211 TLDMDNICFHLEDKTGGKVIYRNINILILYRGRNYDPKQRPQIPLMLWKPLAPIYPRLVQ 270

Query: 278 TTIDGLSINETKEMRKKGLAVPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGLEKS 337
              DGL+  +TKE+R  GL    L +L +NG Y ++V  VR+AF + E+VR+DC  +  S
Sbjct: 271 NVADGLTFEKTKELRNTGLNSSPLMKLTRNGVYVNVVDRVREAFKTVEVVRLDCSHVGSS 330

Query: 338 DYKKIGCKLRDLVPCILVTFEKEQIVVWRGK 363
           D KKIG KLRDLVPC+ + F+ EQI++WRGK
Sbjct: 331 DCKKIGVKLRDLVPCVPLLFKDEQIILWRGK 350

BLAST of CmoCh14G020620 vs. ExPASy Swiss-Prot
Match: Q5VMQ5 (CRS2-associated factor 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0495900 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 2.3e-80
Identity = 154/309 (49.84%), Postives = 197/309 (63.75%), Query Frame = 0

Query: 82  PFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG-----------------VCAP 141
           PF+FQYSYTET    RP+ LRE  F PFGP    R WTG                 +  P
Sbjct: 91  PFEFQYSYTETP-RARPVALREAPFLPFGPEVTPRPWTGRKPLPKSRKELPEFDSFMLPP 150

Query: 142 AVDPKVSSLEST------EDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQI 201
                V  ++S        +P+ +   R   E + GEPLT  E   LV+     KTKRQ+
Sbjct: 151 PGKKGVKPVQSPGPFLAGTEPRYQAASR---EEVLGEPLTKEEVDELVKA--TLKTKRQL 210

Query: 202 NLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFL 261
           N+GRDGLTHNML +I++HWK     +IKC GV TVDM NVC QLEEK  GK+IH  GG +
Sbjct: 211 NIGRDGLTHNMLENIHSHWKRKRVCKIKCKGVCTVDMDNVCQQLEEKVGGKVIHHQGGVI 270

Query: 262 VLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRL 321
            L+RGRNYN + RP  PLMLW+P  PVYPRL+K   DGL+ +E ++MRK+G  +P + +L
Sbjct: 271 FLFRGRNYNYRTRPIYPLMLWKPAAPVYPRLVKKIPDGLTPDEAEDMRKRGRQLPPICKL 330

Query: 322 AKNGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVV 368
            KNG Y +LV  VR+AF +C+LVR+DC GL KSD +KIG KL+DLVPC L++FE E I++
Sbjct: 331 GKNGVYLNLVKQVREAFEACDLVRVDCSGLNKSDCRKIGAKLKDLVPCTLLSFEFEHILM 390

BLAST of CmoCh14G020620 vs. ExPASy TrEMBL
Match: A0A6J1E8W9 (CRS2-associated factor 1, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431873 PE=4 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.1e-245
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK 60
           MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK
Sbjct: 1   MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK 60

Query: 61  PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG 120
           PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG
Sbjct: 61  PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG 120

Query: 121 VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL 180
           VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL
Sbjct: 121 VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL 180

Query: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL 240
           GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL
Sbjct: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL 240

Query: 241 YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK 300
           YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK
Sbjct: 241 YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK 300

Query: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR 360
           NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR
Sbjct: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR 360

Query: 361 GKDYQPSESECFLTVRESFDDVDGNTNCVDNDDDNQNFDDECSDSGFQSGNNE 414
           GKDYQPSESECFLTVRESFDDVDGNTNCVDNDDDNQNFDDECSDSGFQSGNNE
Sbjct: 361 GKDYQPSESECFLTVRESFDDVDGNTNCVDNDDDNQNFDDECSDSGFQSGNNE 413

BLAST of CmoCh14G020620 vs. ExPASy TrEMBL
Match: A0A6J1IVX0 (CRS2-associated factor 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111479719 PE=4 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 5.2e-232
Identity = 390/411 (94.89%), Postives = 400/411 (97.32%), Query Frame = 0

Query: 1   MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK 60
           MFVIRLFRQ+PIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK
Sbjct: 1   MFVIRLFRQDPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK 60

Query: 61  PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG 120
           PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETS  VRPIGLREPKFSPFGPGRLDRKWTG
Sbjct: 61  PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSRLVRPIGLREPKFSPFGPGRLDRKWTG 120

Query: 121 VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL 180
           VCAPAVDPK SSLES EDPKLEEKRRVMWENIQGEPLTGAERK LVETCQKKKTKRQINL
Sbjct: 121 VCAPAVDPKASSLESMEDPKLEEKRRVMWENIQGEPLTGAERKALVETCQKKKTKRQINL 180

Query: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL 240
           GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL
Sbjct: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL 240

Query: 241 YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK 300
           YRGRNYNPKKRPFIPLMLWRPHEP+YPRLIKTTIDGLSI+ETKEMRKKGLAVPALTRL+K
Sbjct: 241 YRGRNYNPKKRPFIPLMLWRPHEPIYPRLIKTTIDGLSIDETKEMRKKGLAVPALTRLSK 300

Query: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR 360
           NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR
Sbjct: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR 360

Query: 361 GKDYQPSESECFLTVRESFDDVDGNTNCVDNDD--DNQNFDDECSDSGFQS 410
           GKDYQPSESECFL+VRESFDD DGNT CVD+DD  D++NFD+ C D+GFQS
Sbjct: 361 GKDYQPSESECFLSVRESFDDFDGNTKCVDSDDDEDDENFDNNCCDAGFQS 411

BLAST of CmoCh14G020620 vs. ExPASy TrEMBL
Match: A0A6J1D1H4 (CRS2-associated factor 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016410 PE=4 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.3e-206
Identity = 358/406 (88.18%), Postives = 371/406 (91.38%), Query Frame = 0

Query: 1   MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK 60
           MFVIRL RQ PI FA P T  NRCFCYSKLRDHYAFESPPSLSPKPP PE KPR NKP K
Sbjct: 1   MFVIRLLRQKPILFAPPLTSFNRCFCYSKLRDHYAFESPPSLSPKPPAPEPKPRPNKPPK 60

Query: 61  PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG 120
            RYKPPSSLDLADKKP+HSDLPFDFQYSYTETS SVRPIGLREPK+SPFGPGRLDRKW+G
Sbjct: 61  QRYKPPSSLDLADKKPRHSDLPFDFQYSYTETSPSVRPIGLREPKYSPFGPGRLDRKWSG 120

Query: 121 VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL 180
           VCAPAVDPK SS+E  EDPKLEEKRR M ENIQGEPLTGAERK LVE CQKKKTKRQINL
Sbjct: 121 VCAPAVDPKASSVEGMEDPKLEEKRRFMRENIQGEPLTGAERKALVEKCQKKKTKRQINL 180

Query: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL 240
           GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLE+KTFGKIIHRHGGFLVL
Sbjct: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEDKTFGKIIHRHGGFLVL 240

Query: 241 YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK 300
           YRGRNY  KKRPFIPLMLWRPHEP+YPRLIKTTIDGLSI ETKEMRKKGLAVPALT+LAK
Sbjct: 241 YRGRNYITKKRPFIPLMLWRPHEPIYPRLIKTTIDGLSIEETKEMRKKGLAVPALTKLAK 300

Query: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR 360
           NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR
Sbjct: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVWR 360

Query: 361 GKDYQPSESECFLTVRESFDD--VDGNTNCVDNDDDNQNFDDECSD 405
           G DY+P +S CFLTVR SFDD   +GNTN V  DDD+ +FDD+ SD
Sbjct: 361 GNDYKPLDSGCFLTVRGSFDDDEANGNTNRVGEDDDDDDFDDDYSD 406

BLAST of CmoCh14G020620 vs. ExPASy TrEMBL
Match: A0A6J1EES7 (CRS2-associated factor 1, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431873 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.6e-203
Identity = 342/348 (98.28%), Postives = 345/348 (99.14%), Query Frame = 0

Query: 1   MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK 60
           MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK
Sbjct: 1   MFVIRLFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPLK 60

Query: 61  PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG 120
           PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG
Sbjct: 61  PRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG 120

Query: 121 VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL 180
           VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL
Sbjct: 121 VCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQINL 180

Query: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL 240
           GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL
Sbjct: 181 GRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVL 240

Query: 241 YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK 300
           YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK
Sbjct: 241 YRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLAK 300

Query: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCIL 349
           NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLR +V  ++
Sbjct: 301 NGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRVMVVSLM 348

BLAST of CmoCh14G020620 vs. ExPASy TrEMBL
Match: A0A5D3D3D7 (CRS2-associated factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004260 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.3e-190
Identity = 331/392 (84.44%), Postives = 354/392 (90.31%), Query Frame = 0

Query: 1   MFVIR-LFRQNPIPFAHPHTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNKPL 60
           MFVIR + R+  IP   PHT  N C  YSKLR+ YAFE P S+SP PP P  KPR NKP 
Sbjct: 1   MFVIRTISREKSIP--RPHTSFNPCSFYSKLRELYAFEFPSSVSPSPPEP--KPRSNKPP 60

Query: 61  KPRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWT 120
           KPRYKPPSSLDL DKKP+ S+LPFDFQYSYTETS SVRPIGLREPK+SPFGPGRLDRKWT
Sbjct: 61  KPRYKPPSSLDLGDKKPRRSNLPFDFQYSYTETSPSVRPIGLREPKYSPFGPGRLDRKWT 120

Query: 121 GVCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQKKKTKRQIN 180
           GVCAPAVDPK +S+E  EDP+LEEKRRVM EN+QGEPLTGAERK LVE CQK KTKRQIN
Sbjct: 121 GVCAPAVDPKATSVEGMEDPRLEEKRRVMRENLQGEPLTGAERKALVEKCQKNKTKRQIN 180

Query: 181 LGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKIIHRHGGFLV 240
           LGRDGLTHNMLNDI+NHWKH EAVRIKCLGVPTVDMKNVCTQLE+KTFGKIIHRHGG LV
Sbjct: 181 LGRDGLTHNMLNDIHNHWKHGEAVRIKCLGVPTVDMKNVCTQLEDKTFGKIIHRHGGLLV 240

Query: 241 LYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLAVPALTRLA 300
           LYRGRNYNPKKRPFIPLMLWRPHEP+YPRLIKTTIDGLSI+ETKEMRKKGLAVPALT+LA
Sbjct: 241 LYRGRNYNPKKRPFIPLMLWRPHEPIYPRLIKTTIDGLSIDETKEMRKKGLAVPALTKLA 300

Query: 301 KNGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTFEKEQIVVW 360
           KNGYYGSLVPMVRDAFLSCELVRIDC+GLE+SDYKKIGCKLRDLVPCILVTF+KEQIVVW
Sbjct: 301 KNGYYGSLVPMVRDAFLSCELVRIDCKGLERSDYKKIGCKLRDLVPCILVTFDKEQIVVW 360

Query: 361 RGKDYQPSESECFLTVRESFDDVDGNTNCVDN 392
           RGKDYQP +   +LTVRE+FDDVDGNT CVD+
Sbjct: 361 RGKDYQPLDIG-YLTVRETFDDVDGNTGCVDD 387

BLAST of CmoCh14G020620 vs. TAIR 10
Match: AT4G31010.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 532.7 bits (1371), Expect = 2.6e-151
Identity = 274/407 (67.32%), Postives = 316/407 (77.64%), Query Frame = 0

Query: 1   MFVIRLFRQNPIPFA------HPHTPSNRCFCYSKLRDHYAFESPPSLS---PKPPPPEL 60
           MF+IRL R NP  F       H  T S+     S+LRD Y F+SPP LS    + P    
Sbjct: 1   MFLIRLSRHNPSSFTLLTRRLHDQTISS-----SRLRDLYNFQSPPPLSSSASENPDFNQ 60

Query: 61  KPRQNKPLKPRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGP 120
           K    K  KP+Y+PPSSL+    K  HSDLPFDF++SYTE+  +VRPIGLREPK+SPFGP
Sbjct: 61  KNNNKKKPKPQYRPPSSLE--GVKTVHSDLPFDFRFSYTESCSNVRPIGLREPKYSPFGP 120

Query: 121 GRLDRKWTGVCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQK 180
            RLDR+WTGVCAPAV+PKV S++  EDPKLEEKRR + E IQG  LT AERK LVE CQ+
Sbjct: 121 DRLDREWTGVCAPAVNPKVESVDGVEDPKLEEKRRKVREKIQGASLTEAERKFLVELCQR 180

Query: 181 KKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKII 240
            KTKRQ+NLGRDGLTHNMLND+YNHWKHAEAVR+KCLGVPT+DMKNV   LE+KTFG+++
Sbjct: 181 NKTKRQVNLGRDGLTHNMLNDVYNHWKHAEAVRVKCLGVPTLDMKNVIFHLEDKTFGQVV 240

Query: 241 HRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLA 300
            +H G LVLYRGRNY+PKKRP IPLMLW+PHEPVYPRLIKTTIDGLSI+ETK MRKKGLA
Sbjct: 241 SKHSGTLVLYRGRNYDPKKRPKIPLMLWKPHEPVYPRLIKTTIDGLSIDETKAMRKKGLA 300

Query: 301 VPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLVPCILVTF 360
           VPALT+LAKNGYYGSLVPMVRDAFL  ELVRIDC GLE+ DYKKIG KLRDLVPCILVTF
Sbjct: 301 VPALTKLAKNGYYGSLVPMVRDAFLVSELVRIDCLGLERKDYKKIGAKLRDLVPCILVTF 360

Query: 361 EKEQIVVWRGKDYQPSESE---CFLTVRESFDDVDGNTNCVDNDDDN 396
           +KEQ+V+WRGKDY+P + +        RES  D D + +C     D+
Sbjct: 361 DKEQVVIWRGKDYKPPKEDDEYSSFIHRESSIDSDVDLSCSRGAQDS 400

BLAST of CmoCh14G020620 vs. TAIR 10
Match: AT4G31010.2 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 408.3 bits (1048), Expect = 7.4e-114
Identity = 209/307 (68.08%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 1   MFVIRLFRQNPIPFA------HPHTPSNRCFCYSKLRDHYAFESPPSLS---PKPPPPEL 60
           MF+IRL R NP  F       H  T S+     S+LRD Y F+SPP LS    + P    
Sbjct: 1   MFLIRLSRHNPSSFTLLTRRLHDQTISS-----SRLRDLYNFQSPPPLSSSASENPDFNQ 60

Query: 61  KPRQNKPLKPRYKPPSSLDLADKKPKHSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGP 120
           K    K  KP+Y+PPSSL+    K  HSDLPFDF++SYTE+  +VRPIGLREPK+SPFGP
Sbjct: 61  KNNNKKKPKPQYRPPSSLE--GVKTVHSDLPFDFRFSYTESCSNVRPIGLREPKYSPFGP 120

Query: 121 GRLDRKWTGVCAPAVDPKVSSLESTEDPKLEEKRRVMWENIQGEPLTGAERKTLVETCQK 180
            RLDR+WTGVCAPAV+PKV S++  EDPKLEEKRR + E IQG  LT AERK LVE CQ+
Sbjct: 121 DRLDREWTGVCAPAVNPKVESVDGVEDPKLEEKRRKVREKIQGASLTEAERKFLVELCQR 180

Query: 181 KKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEEKTFGKII 240
            KTKRQ+NLGRDGLTHNMLND+YNHWKHAEAVR+KCLGVPT+DMKNV   LE+KTFG+++
Sbjct: 181 NKTKRQVNLGRDGLTHNMLNDVYNHWKHAEAVRVKCLGVPTLDMKNVIFHLEDKTFGQVV 240

Query: 241 HRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKEMRKKGLA 299
            +H G LVLYRGRNY+PKKRP IPLMLW+PHEPVYPRLIKTTIDGLSI+ETK MRKKGLA
Sbjct: 241 SKHSGTLVLYRGRNYDPKKRPKIPLMLWKPHEPVYPRLIKTTIDGLSIDETKAMRKKGLA 300

BLAST of CmoCh14G020620 vs. TAIR 10
Match: AT5G54890.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 339.3 bits (869), Expect = 4.2e-93
Identity = 176/334 (52.69%), Postives = 226/334 (67.66%), Query Frame = 0

Query: 32  DHYAFESPPSLSPKPPPPELKPRQNKPLKPRYKPPSSLDLAD--KKPKHSDLPFDFQYSY 91
           D Y     P   P  PP E K ++ K      K   S +L +  K P  SDLPFDF+YSY
Sbjct: 32  DDYDPPFSPLSKPTKPPKEKKKQKTK------KQDQSSELVNDLKIPVISDLPFDFRYSY 91

Query: 92  TETSLSVRPIGLREPK-FSPFGPGRLDRKWTGVCAPAVDPKVSSLESTEDPKLEEKRRVM 151
           +ET+  + PIG REPK FSPFGPGRLDRKWTG  A      ++S E  +   +EE+ RV+
Sbjct: 92  SETNPEIEPIGFREPKRFSPFGPGRLDRKWTGTTA------LASPEIDQSQWVEERARVL 151

Query: 152 WENIQGEPLTGAERKTLVETCQKKKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCL 211
                GE LT  E   L+E  +     RQINLG+ G+THNM++DI+NHWK AEAVRIKCL
Sbjct: 152 -----GETLTEDEVTELIERYRHSDCTRQINLGKGGVTHNMIDDIHNHWKKAEAVRIKCL 211

Query: 212 GVPTVDMKNVCTQLEEKTFGKIIHRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPR 271
           GVPT+DM N+C  LEEK+ GKI++R+   LVLYRGRNY+PK RP IPLMLW+PH P+YPR
Sbjct: 212 GVPTLDMDNICFHLEEKSGGKIVYRNINILVLYRGRNYDPKSRPIIPLMLWKPHPPIYPR 271

Query: 272 LIKTTIDGLSINETKEMRKKGLAVPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGL 331
           L+K   DGL   ETKEMR +GL  PAL +L +NG Y ++V  VR+ F + E+VR+DC  +
Sbjct: 272 LVKNVADGLEFEETKEMRNRGLHSPALMKLTRNGVYVNVVGRVREEFETEEIVRLDCTHV 331

Query: 332 EKSDYKKIGCKLRDLVPCILVTFEKEQIVVWRGK 363
             SD K+IG KL+++VPC+ + F+ EQI++WRGK
Sbjct: 332 GMSDCKRIGVKLKEMVPCVPILFKDEQIILWRGK 348

BLAST of CmoCh14G020620 vs. TAIR 10
Match: AT2G20020.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.4e-80
Identity = 170/386 (44.04%), Postives = 226/386 (58.55%), Query Frame = 0

Query: 18  HTPSNRCFCYSKLRDHYAFESPPSLSPKPPPPELKPRQNK----PLKPRYKP--PSSLDL 77
           HT  +           +     PSL      P  + + +K    P +P+ KP  P S   
Sbjct: 69  HTHDSEAAAAEPKTSPFRSRGTPSLPSARSIPGRRSKYSKPDSGPNRPKNKPRVPDSPPQ 128

Query: 78  ADKKPK-----------HSDLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTG 137
            D KP+            +  PF+F+YSYTET   V+P+ LREP ++PFGP  + R WTG
Sbjct: 129 LDAKPEVKLSEDGLTYVINGAPFEFKYSYTETP-KVKPLKLREPAYAPFGPTTMGRPWTG 188

Query: 138 -VCAPAVDPKVSSLESTEDPKLEEK----------------RRVMW--ENIQGEPLTGAE 197
               P         +S   P + +K                 R ++  E I GEPLT  E
Sbjct: 189 RAPLPQSQKTPREFDSFRLPPVGKKGLKPVQKPGPFRPGVGPRYVYSKEEILGEPLTKEE 248

Query: 198 RKTLVETCQKKKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQ 257
            + LV +C   KT RQ+N+GRDGLTHNMLN+I++ WK     +IKC GV TVDM NVC Q
Sbjct: 249 VRELVTSC--LKTTRQLNMGRDGLTHNMLNNIHDLWKRRRVCKIKCKGVCTVDMDNVCEQ 308

Query: 258 LEEKTFGKIIHRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINE 317
           LEEK  GK+I+R GG L L+RGRNYN + RP  PLMLW+P  PVYPRLI+   +GL+  E
Sbjct: 309 LEEKIGGKVIYRRGGVLFLFRGRNYNHRTRPRFPLMLWKPVAPVYPRLIQQVPEGLTRQE 368

Query: 318 TKEMRKKGLAVPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLR 368
              MR+KG  +  + +L KNG Y  LV  V++AF  CELVRIDCQG++ SD++KIG KL+
Sbjct: 369 ATNMRRKGRELMPICKLGKNGVYCDLVKNVKEAFEVCELVRIDCQGMKGSDFRKIGAKLK 428

BLAST of CmoCh14G020620 vs. TAIR 10
Match: AT1G23400.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-74
Identity = 160/381 (41.99%), Postives = 220/381 (57.74%), Query Frame = 0

Query: 11  PIPFAHPHTPSNRCFCYSKLRDHYAFESP--PSLSPKPPPPELKPRQNKPLKP---RYKP 70
           PIP    + PSNR    ++  +H    +P  P  +P    P  + R  KP+K        
Sbjct: 35  PIPIP-KYAPSNR--NRNQKTNHQTDTNPKKPQSNPALKLPHHRTRYYKPVKEGVISSDG 94

Query: 71  PSSLDLADKKPKHS--DLPFDFQYSYTETSLSVRPIGLREPKFSPFGPGRLDRKWTGVCA 130
             ++ + D    +     PF+FQ+SY+ET   V+P+G+REP F PF P  + R WTG   
Sbjct: 95  DRTILIGDSGVSYQLPGAPFEFQFSYSETP-KVKPVGIREPAFMPFAPPTMPRPWTGKAP 154

Query: 131 PAVDPKVSSLESTEDPKLEEKRRVMW-------------------ENIQGEPLTGAERKT 190
                K   L  + +P    K  V +                   E + GEPL   E+  
Sbjct: 155 LKKSKKKIPLFDSFNPPPAGKSGVKYVEMPGPLPFGRYPKEGMNREEVLGEPLKRWEKGM 214

Query: 191 LVETCQKKKTKRQINLGRDGLTHNMLNDIYNHWKHAEAVRIKCLGVPTVDMKNVCTQLEE 250
           L++        RQ+NLGRDG THNML  I++HWK     +++C GVPTVDM NVC  LEE
Sbjct: 215 LIK--PHMHDNRQVNLGRDGFTHNMLELIHSHWKRRRVCKVRCKGVPTVDMNNVCRVLEE 274

Query: 251 KTFGKIIHRHGGFLVLYRGRNYNPKKRPFIPLMLWRPHEPVYPRLIKTTIDGLSINETKE 310
           KT G+IIHR GG + L+RGRNYN + RP  PLMLW+P  PVYP+LI+   +GL+  E  E
Sbjct: 275 KTGGEIIHRVGGVVYLFRGRNYNYRTRPQYPLMLWKPAAPVYPKLIQEVPEGLTKEEAHE 334

Query: 311 MRKKGLAVPALTRLAKNGYYGSLVPMVRDAFLSCELVRIDCQGLEKSDYKKIGCKLRDLV 366
            R KG ++  + +L+KNG Y SLV  VRDAF    LV++DC GLE SDYKKIG KL++LV
Sbjct: 335 FRVKGKSLRPICKLSKNGVYVSLVKDVRDAFELSSLVKVDCPGLEPSDYKKIGAKLKELV 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYD93.6e-15067.32CRS2-associated factor 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g3... [more]
Q6Z4U23.8e-13161.29CRS2-associated factor 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947... [more]
Q9FFU15.9e-9252.69CRS2-associated factor 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g5... [more]
Q0J7J76.8e-8850.45CRS2-associated factor 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947... [more]
Q5VMQ52.3e-8049.84CRS2-associated factor 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947... [more]
Match NameE-valueIdentityDescription
A0A6J1E8W91.1e-245100.00CRS2-associated factor 1, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1IVX05.2e-23294.89CRS2-associated factor 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111479... [more]
A0A6J1D1H41.3e-20688.18CRS2-associated factor 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1EES76.6e-20398.28CRS2-associated factor 1, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662... [more]
A0A5D3D3D71.3e-19084.44CRS2-associated factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
Match NameE-valueIdentityDescription
AT4G31010.12.6e-15167.32RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT4G31010.27.4e-11468.08RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT5G54890.14.2e-9352.69RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT2G20020.12.4e-8044.04RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT1G23400.11.5e-7441.99RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001890RNA-binding, CRM domainSMARTSM01103CRS1_YhbY_2coord: 277..360
e-value: 9.1E-11
score: 51.8
coord: 157..242
e-value: 1.1E-18
score: 78.1
IPR001890RNA-binding, CRM domainPFAMPF01985CRS1_YhbYcoord: 157..242
e-value: 7.3E-20
score: 70.9
coord: 280..360
e-value: 4.4E-9
score: 36.4
IPR001890RNA-binding, CRM domainPROSITEPS51295CRMcoord: 155..253
score: 19.06831
IPR001890RNA-binding, CRM domainPROSITEPS51295CRMcoord: 275..371
score: 16.926235
IPR035920YhbY-like superfamilyGENE3D3.30.110.60coord: 156..253
e-value: 5.1E-23
score: 83.0
IPR035920YhbY-like superfamilyGENE3D3.30.110.60coord: 277..371
e-value: 1.9E-12
score: 49.0
IPR035920YhbY-like superfamilySUPERFAMILY75471YhbY-likecoord: 156..252
IPR035920YhbY-like superfamilySUPERFAMILY75471YhbY-likecoord: 277..366
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 390..413
NoneNo IPR availablePANTHERPTHR46247:SF2CRS2-ASSOCIATED FACTOR 1, MITOCHONDRIALcoord: 22..396
IPR044599CRS2-associated factor, plantPANTHERPTHR46247CRS2-ASSOCIATED FACTOR 1, CHLOROPLASTICcoord: 22..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh14G020620.1CmoCh14G020620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000373 Group II intron splicing
biological_process GO:0006397 mRNA processing
molecular_function GO:0003723 RNA binding