CmoCh14G013890 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh14G013890
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDelta 9 desaturase
LocationCmo_Chr14: 11448393 .. 11450445 (-)
RNA-Seq ExpressionCmoCh14G013890
SyntenyCmoCh14G013890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGACTGTTTCTTATCCATTTGATTATGGAAGCAACAAGGAAAGAGGTGGAAGATAAATATGATAAGAAGAAGCCATTTTCAGTGGTGAACCCTAAACGCCCATTTCTAAACAGAAAATGGACAACTTTAGACCGACGTAATGCTGCTGGAATTTTGTTCTTGCATGTTCTTGGTCTCTTCGCCCCATTTCACTACAATTGGCCAGCCTTTTGGGTTGCCTTTACTCTATATTTTACTACAGGCGTGCTGGGGATAACGCTCTCATATCATAGAAATCTCGCTCATAGAAGTTTTAAACTTCCCAAATGGCTCGAGTACTCGTTTGCTTATCTTGGAGTGCAAGCCTTTCAGGTAATTTATCGTCTTAGTATCTTAGGTTCAAAGTATCTCGATTTAGATTTAGCTATAAATATTTTTTTTTTGTTTTTGTTATAGGGGGATCCAATTGATTGGGTTAGTACGCATAGATATCACCATCAGTTTGTTGATACAGAACGAGATCCACATAGTCCTTTAGAAGGGTTTTGGTTTAGTCACGTAACTTGGATGTTTGACTCGTATGGTTTGACTAATAAGATTTACCCAAAATACCTCGAAGATTTTGAGGAACGAGATGCTAAGAGAACACTTTCTCGCAATTGGTTCGTGGGGTTTCTAAAGTATGGAAGACCAAAGAATGTAGAGGATTTGCAGAAAGAAGGTTTTTATAGGTTCCTACGAAAAACATACCTTCTTCATCCTATAGCTCTTGCAATGGTCCTGTATGGATTTGGAGGACTTCCATTTGTTGTTTGGGGAATGGTAAGTATTAATAAACCATTGCTTTCAATTGTTGGTTATTACAATTAGACATTCAAAATGGATATGCTTTGGCGATGGCAGGGAGTGAGGCTTATTTGTGTGCTGCACGCTGCTTTCTTCGTAAACTCAGCATGCCACATCTGGGGCTATCGGAAATGGAACACCAATGATTTGTCAAAGAACAATTGGTATTGTCAATCCTTCTTCTTAATTTTTTTTTTTTTCATGTGGGTTTTAAATGTGTAAATTTGTTTTGAATTATAGTGTCTAATTCATCTCTAATTTATTATGTGATACTCTAATCTAATAAAAGAGAGGGTATATGCAACCATACAAGCTGGATTTTCCTTTCGGGCTTCAGCTCAAGGTTTTAAAACGTGTTTGCTTCCCTTCTCCAATCGAGGTGGGATCTCACAATTCACCCCCCTTTCCCTTTCAGAGCCTAGCGTCCTCTCTAACACTCTTTAGCCAAATTCAATTGATGTGGAATCTCACAATACACCATCTTTTGGGTACCAGCGTCTTCGCTCGCACACTGTCCAGAAGTTGGCTATGATACCATTTGTAACAGCAAGTCCTCCCCTAGCAGATTTGTCCTCTTTGGACTTCCTCTCAAAAATTTTAAAACGCTTATACTAAATAGAGGTTTCCACACCCTTATAAAGAATGTTTCGTTCTCTTCTCCAACAAATGTGAGATCTCACAGTATATGAATGAACCAAGATCACATCCAAATGAGAGAAATCTTGAGAATAATATAAAAAAATTTAAAAGAATGAGAGTTACTTCTTATACTAACATGGTAACATGGTGTACATTTATTATCAGTAGCTCAATCATAGAAACTCCAAAATTAAATGGGTTATGTATTAAACATTTCTAAACTAATTTCTTATTTTATATCTTGTTTAATTAACATTGTGTGTTTATACGTTGACACAGGGTGATTGGGTTGGTGTCGTGTGGGGAAGGTTGGCATAATAACCACCATGCTTTTGAATACTCAGCTCGACATGGACTTCAATGGTGGGAATTTGACTTCACGTGGTGGGTCATATTATTACTTCAAGCTCTTGGTTTGGCAACTGATGTAAAATTACCCTCTCAACGTCATATGCAAAAGTTGGCCATCCAAGACAAAATCACTTCACCCTGATTTCTTTCGAGTATTATTGACTTATGCACTAATTAGTATTCAGCTGTTTACCTAAAAATAAATCCATTTGTCCCTTTGAGACCAGTGTTTGTCTG

mRNA sequence

TTGACTGTTTCTTATCCATTTGATTATGGAAGCAACAAGGAAAGAGGTGGAAGATAAATATGATAAGAAGAAGCCATTTTCAGTGGTGAACCCTAAACGCCCATTTCTAAACAGAAAATGGACAACTTTAGACCGACGTAATGCTGCTGGAATTTTGTTCTTGCATGTTCTTGGTCTCTTCGCCCCATTTCACTACAATTGGCCAGCCTTTTGGGTTGCCTTTACTCTATATTTTACTACAGGCGTGCTGGGGATAACGCTCTCATATCATAGAAATCTCGCTCATAGAAGTTTTAAACTTCCCAAATGGCTCGAGTACTCGTTTGCTTATCTTGGAGTGCAAGCCTTTCAGGGGGATCCAATTGATTGGGTTAGTACGCATAGATATCACCATCAGTTTGTTGATACAGAACGAGATCCACATAGTCCTTTAGAAGGGTTTTGGTTTAGTCACGTAACTTGGATGTTTGACTCGTATGGTTTGACTAATAAGATTTACCCAAAATACCTCGAAGATTTTGAGGAACGAGATGCTAAGAGAACACTTTCTCGCAATTGGTTCGTGGGGTTTCTAAAGTATGGAAGACCAAAGAATGTAGAGGATTTGCAGAAAGAAGGTTTTTATAGGTTCCTACGAAAAACATACCTTCTTCATCCTATAGCTCTTGCAATGGTCCTGTATGGATTTGGAGGACTTCCATTTGTTGTTTGGGGAATGGGAGTGAGGCTTATTTGTGTGCTGCACGCTGCTTTCTTCGTAAACTCAGCATGCCACATCTGGGGCTATCGGAAATGGAACACCAATGATTTGTCAAAGAACAATTGGGTGATTGGGTTGGTGTCGTGTGGGGAAGGTTGGCATAATAACCACCATGCTTTTGAATACTCAGCTCGACATGGACTTCAATGGTGGGAATTTGACTTCACGTGGTGGGTCATATTATTACTTCAAGCTCTTGGTTTGGCAACTGATGTAAAATTACCCTCTCAACGTCATATGCAAAAGTTGGCCATCCAAGACAAAATCACTTCACCCTGATTTCTTTCGAGTATTATTGACTTATGCACTAATTAGTATTCAGCTGTTTACCTAAAAATAAATCCATTTGTCCCTTTGAGACCAGTGTTTGTCTG

Coding sequence (CDS)

ATGGAAGCAACAAGGAAAGAGGTGGAAGATAAATATGATAAGAAGAAGCCATTTTCAGTGGTGAACCCTAAACGCCCATTTCTAAACAGAAAATGGACAACTTTAGACCGACGTAATGCTGCTGGAATTTTGTTCTTGCATGTTCTTGGTCTCTTCGCCCCATTTCACTACAATTGGCCAGCCTTTTGGGTTGCCTTTACTCTATATTTTACTACAGGCGTGCTGGGGATAACGCTCTCATATCATAGAAATCTCGCTCATAGAAGTTTTAAACTTCCCAAATGGCTCGAGTACTCGTTTGCTTATCTTGGAGTGCAAGCCTTTCAGGGGGATCCAATTGATTGGGTTAGTACGCATAGATATCACCATCAGTTTGTTGATACAGAACGAGATCCACATAGTCCTTTAGAAGGGTTTTGGTTTAGTCACGTAACTTGGATGTTTGACTCGTATGGTTTGACTAATAAGATTTACCCAAAATACCTCGAAGATTTTGAGGAACGAGATGCTAAGAGAACACTTTCTCGCAATTGGTTCGTGGGGTTTCTAAAGTATGGAAGACCAAAGAATGTAGAGGATTTGCAGAAAGAAGGTTTTTATAGGTTCCTACGAAAAACATACCTTCTTCATCCTATAGCTCTTGCAATGGTCCTGTATGGATTTGGAGGACTTCCATTTGTTGTTTGGGGAATGGGAGTGAGGCTTATTTGTGTGCTGCACGCTGCTTTCTTCGTAAACTCAGCATGCCACATCTGGGGCTATCGGAAATGGAACACCAATGATTTGTCAAAGAACAATTGGGTGATTGGGTTGGTGTCGTGTGGGGAAGGTTGGCATAATAACCACCATGCTTTTGAATACTCAGCTCGACATGGACTTCAATGGTGGGAATTTGACTTCACGTGGTGGGTCATATTATTACTTCAAGCTCTTGGTTTGGCAACTGATGTAAAATTACCCTCTCAACGTCATATGCAAAAGTTGGCCATCCAAGACAAAATCACTTCACCCTGA

Protein sequence

MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP
Homology
BLAST of CmoCh14G013890 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 396.4 bits (1017), Expect = 3.3e-109
Identity = 175/300 (58.33%), Postives = 217/300 (72.33%), Query Frame = 0

Query: 30  RKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRS 89
           R+W  +D    A +L +H+L L APF +NW A  VAF LY  TG+LGITLS+HRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 90  FKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHVTWMFD 149
           FKLPKWLEY FAY G QA QG+PIDWVSTHRYHHQF D++RDPHSPL+GFWFSH+ WMFD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 150 SYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLL 209
           +  +T +                             G P NV DL+K+ FYRFLR TY+L
Sbjct: 216 TNTITQRC----------------------------GEPNNVGDLEKQPFYRFLRTTYIL 275

Query: 210 HPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVI 269
           HP+ALA+ LY  GG PF+VWGMGVR++ V H  + VNSACH+WG + WNT DLSKNNW +
Sbjct: 276 HPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWV 335

Query: 270 GLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKLPSQRHMQKLA 329
             ++ GEGWHNNHHAFE+SARHGL+WW+ D TW+V+  LQA+GLATDVKLPS+   Q++A
Sbjct: 336 AALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of CmoCh14G013890 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.4e-96
Identity = 162/303 (53.47%), Postives = 206/303 (67.99%), Query Frame = 0

Query: 30  RKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRS 89
           RKW + D  +   I  LHVL LFAP  ++W +FWV F LY   GV G TLS+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 90  FKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHVTWMFD 149
           FKLPK+LEY FAY+G+ A QGDP+ WVSTHRYHH++ DT  DPHSP+EGFWF H+ W+FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 150 SYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLL 209
           S         KY+ +                   K GR +N  DL K+ +YRFL +T++ 
Sbjct: 158 S---------KYIIE-------------------KCGRYENAGDLMKQSYYRFLERTFVY 217

Query: 210 HPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVI 269
           H    A +LY FGG PF+VWGM VR I   H ++ VNS CH WG R WNT DLS NNW I
Sbjct: 218 HVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFI 277

Query: 270 GLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKLPSQRHMQKLA 329
            +++ GEGWHNNHHAFEYSARHG++WW+ D TW++I LL+ LGLATD+K+PS+ H +K++
Sbjct: 278 AMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMS 312

Query: 330 IQD 333
            ++
Sbjct: 338 FKN 312

BLAST of CmoCh14G013890 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 9.4e-96
Identity = 164/308 (53.25%), Postives = 211/308 (68.51%), Query Frame = 0

Query: 30  RKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRS 89
           R W + D  +   +  LHVL L APF ++W AFW++ TLY   GV G TLSYHRNL HRS
Sbjct: 35  RVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRS 94

Query: 90  FKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHVTWMFD 149
           FKLPK+LEY FAY+G+ A QGDP+ WVSTHRYHH+F DT  DPHSP+EGFWFSH+ W+FD
Sbjct: 95  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFD 154

Query: 150 SYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLL 209
           S         KY+ +                   + GR +N  DL K+ +YRFL +T++ 
Sbjct: 155 S---------KYILE-------------------ECGRYENAGDLLKQSYYRFLERTFVF 214

Query: 210 HPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVI 269
           H    A +LY FGG PF+VWGM VR +   HA++ VNS CH +G++ W+T DLS NNW I
Sbjct: 215 HVYLQAALLYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFI 274

Query: 270 GLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKLPSQRHMQKLA 329
            +++ GEGWHNNHHAFEYSARHG++WW+ D TW+VI LL+ LGLATDVK+PS+ H +K++
Sbjct: 275 AMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHKRKMS 314

Query: 330 ----IQDK 334
               +QDK
Sbjct: 335 FKNCVQDK 314

BLAST of CmoCh14G013890 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.7e-95
Identity = 164/303 (54.13%), Postives = 202/303 (66.67%), Query Frame = 0

Query: 27  FLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLY-FTTGVLGITLSYHRNL 86
           F  RKW   D +NA  +L LH L   APF+++W AFW++F L  F +GVLGITL +HR L
Sbjct: 76  FWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLCFHRCL 135

Query: 87  AHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHVT 146
            H  FKLPK +EY FAY G  A QGDP++WVS HRYHHQFVDTERD HSP +GFWF H+ 
Sbjct: 136 THGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWFCHIG 195

Query: 147 WMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGFYRFLRK 206
           W+ D             + F E+               + GR  NV DL+K+ FYRFL+K
Sbjct: 196 WVLDK------------DLFVEK---------------RGGRRNNVNDLKKQAFYRFLQK 255

Query: 207 TYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNTNDLSKN 266
           TY+ H +AL  +LY  GG P++VWGMG RL+ + H+ F +NS CH WG R WNT DLS N
Sbjct: 256 TYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTN 315

Query: 267 NWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKLPSQRHM 326
           N  + L + GEGWHNNHHAFE SARHGL+WWE D TW+VI  LQA+GLAT+VKLP++   
Sbjct: 316 NMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPTEAQK 351

Query: 327 QKL 329
           QKL
Sbjct: 376 QKL 351

BLAST of CmoCh14G013890 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 347.8 bits (891), Expect = 1.4e-94
Identity = 159/311 (51.13%), Postives = 206/311 (66.24%), Query Frame = 0

Query: 20  VVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITL 79
           VV  KR F  R WT+ D       +  H+L L APF+++W AFWV   L F  G+  ITL
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 80  SYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGF 139
           SYHRNL+HRSF LPKWLEY FAY GV AFQGDPI+WVS HRYHH+  +T+RDPHSP +GF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 140 WFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGF 199
           WFSH+ W+FD+  +                                G  +NV+DL ++ F
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPF 258

Query: 200 YRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNT 259
           YRFL++T LLH +A + + Y  GG+P +VWG+G+ +   LH  F VNS CHIWG R WNT
Sbjct: 259 YRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNT 318

Query: 260 NDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKL 319
           +D SKNNW + +++ GEGWHNNHHAFE+SARHGL+WW+ D TW +I  L+A+GLAT+VKL
Sbjct: 319 SDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKL 360

Query: 320 PSQRHMQKLAI 331
           P++  M+  A+
Sbjct: 379 PTETQMKGKAL 360

BLAST of CmoCh14G013890 vs. ExPASy TrEMBL
Match: A0A6J1GVF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457787 PE=3 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.4e-206
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP
Sbjct: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
           AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 338
           TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP
Sbjct: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 337

BLAST of CmoCh14G013890 vs. ExPASy TrEMBL
Match: A0A0A0KWV4 (Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.5e-184
Identity = 300/337 (89.02%), Postives = 315/337 (93.47%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           MEA RKEVEDK+DKKK FS+VNPKR F NRKWTTLDRR AAGILFLH+L L APFH+NW 
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPFHFNWA 60

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAF LY  TG LGITLSYHRNLAHRSFKLPKWLEY FAYLGVQ FQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHHQFVDT+RDPHSP++GFWFSH+TWMFDSYGLT KIYPK LEDF+ER+AKRTLSRNWFV
Sbjct: 121 YHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQK+GFYRFLRKTYLLHPIALA+VLY FGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
           AA FVNSACHIWGYRKWNTNDLSKNNW+IGL+S GEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AALFVNSACHIWGYRKWNTNDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 338
           TWWVILLLQALGLATDVKLP+Q H QKLAIQ KITSP
Sbjct: 301 TWWVILLLQALGLATDVKLPTQLHKQKLAIQYKITSP 337

BLAST of CmoCh14G013890 vs. ExPASy TrEMBL
Match: A0A6J1DIZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020970 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.1e-160
Identity = 262/332 (78.92%), Postives = 290/332 (87.35%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           MEATRKEVED+ DKK  FSVV P+RP L RKWT LDRR+A  ILFLH+L L APFH NW 
Sbjct: 1   MEATRKEVEDQKDKKVAFSVVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWA 60

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAF LYF TG+ GITLSYHRNLAH+SFKLPKWLEY+FAYLGV AFQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHH+FVDTERDPHSPLEGFWFSH+ WMFD+Y LT K+ PK ++DF+ERDAKR    N FV
Sbjct: 121 YHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFV 180

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
           GF+KYGRP NVEDLQK+ FYRFLRKTY+LHP ALA+VLY  GGLPF+VWGMGVRL+ VLH
Sbjct: 181 GFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLH 240

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
             FFVNSACHIWGYR+W+TNDLSKNNW IGL++ GEGWHNNHHAFEYSARHGLQWWEFD 
Sbjct: 241 ITFFVNSACHIWGYRRWDTNDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQWWEFDL 300

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQD 333
           TW V+L+LQALGLATDVKLPSQ H QKLA+ +
Sbjct: 301 TWCVVLVLQALGLATDVKLPSQIHKQKLALHN 332

BLAST of CmoCh14G013890 vs. ExPASy TrEMBL
Match: A0A6J1DIX0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020949 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.4e-155
Identity = 257/337 (76.26%), Postives = 285/337 (84.57%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKK-PFS---VVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFH 60
           MEATRKE+EDK + KK  FS   V+ P+R FL RKWT LDRR+A GILFLH+L L APFH
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIVLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFH 60

Query: 61  YNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWV 120
            NW AFWVAF LYF TG+ GITLSYHRNLAH+SFKLPKWLEY FAYLG+ AFQGDPIDWV
Sbjct: 61  CNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWV 120

Query: 121 STHRYHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSR 180
           STHRYHHQFVDTERDPHSPLEGFWFSH+TWMFD+Y LT K+ PK +EDFEE+DAKR    
Sbjct: 121 STHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFS 180

Query: 181 NWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLI 240
           N FV  +KYGRP NV+DLQK+ FYRFLR+TY+LHP ALA+VLY  GGLPFVVWGMGVRL+
Sbjct: 181 NIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLV 240

Query: 241 CVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWW 300
             LH  FF+NSACHIWGYR+WNT DLSKNNWV GL++ GEGWHNNHHAFEYSARHGLQWW
Sbjct: 241 WFLHITFFINSACHIWGYRRWNTTDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWW 300

Query: 301 EFDFTWWVILLLQALGLATDVKLPSQRHMQKLAIQDK 334
           EFD TWWV+L LQ +GLATDVKLPSQ   QKLA+  K
Sbjct: 301 EFDLTWWVVLFLQTIGLATDVKLPSQGQKQKLAMDHK 337

BLAST of CmoCh14G013890 vs. ExPASy TrEMBL
Match: A0A6J1GCE1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452892 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.6e-153
Identity = 249/320 (77.81%), Postives = 273/320 (85.31%), Query Frame = 0

Query: 14  KKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTG 73
           KK   S+V  K   LNRKWT LDRR+A G+LFLH L L APFH NWPAFWVAF LYF TG
Sbjct: 5   KKVALSMVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAFALYFITG 64

Query: 74  VLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPH 133
           + G+TLSYHRNLAH+SFKLPKWLEY FAYLGVQAFQGDPIDWVSTHRYHHQFVDT+RDPH
Sbjct: 65  LFGVTLSYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFVDTDRDPH 124

Query: 134 SPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVED 193
           SPL+GFWFSH+ WMFD+Y LT K+YPK ++DFEER  KR   RN  VG++KYGRP NVED
Sbjct: 125 SPLDGFWFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYGRPNNVED 184

Query: 194 LQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWG 253
           LQK+GFYRFLRKTY+LHP A A+VLY  GGLPFVVWGMGVRL+ VLH  FFVNSACHIWG
Sbjct: 185 LQKQGFYRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVNSACHIWG 244

Query: 254 YRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGL 313
           YR W+TNDLSKNNWVIGL++ GEGWHNNHHAFEYSARHG +WWEFD TW VIL LQALGL
Sbjct: 245 YRLWDTNDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTWCVILFLQALGL 304

Query: 314 ATDVKLPSQRHMQKLAIQDK 334
           ATDVKLPS RH QKLA+  K
Sbjct: 305 ATDVKLPSARHQQKLALNPK 324

BLAST of CmoCh14G013890 vs. NCBI nr
Match: XP_022955953.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata] >KAG6581939.1 Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 728.4 bits (1879), Expect = 2.8e-206
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP
Sbjct: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
           AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 338
           TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP
Sbjct: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 337

BLAST of CmoCh14G013890 vs. NCBI nr
Match: XP_023528301.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.2 bits (1868), Expect = 5.3e-205
Identity = 333/337 (98.81%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           MEATRKEVEDKYDKKKPFS+VNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP
Sbjct: 1   MEATRKEVEDKYDKKKPFSMVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR
Sbjct: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKY+EDFEERDAKRTLSRNWFV
Sbjct: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYVEDFEERDAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQK+GFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
           AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHG+QWWEFDF
Sbjct: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGIQWWEFDF 300

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 338
           TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP
Sbjct: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 337

BLAST of CmoCh14G013890 vs. NCBI nr
Match: XP_004152341.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KGN52907.1 hypothetical protein Csa_014904 [Cucumis sativus])

HSP 1 Score: 655.2 bits (1689), Expect = 3.0e-184
Identity = 300/337 (89.02%), Postives = 315/337 (93.47%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           MEA RKEVEDK+DKKK FS+VNPKR F NRKWTTLDRR AAGILFLH+L L APFH+NW 
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPFHFNWA 60

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAF LY  TG LGITLSYHRNLAHRSFKLPKWLEY FAYLGVQ FQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHHQFVDT+RDPHSP++GFWFSH+TWMFDSYGLT KIYPK LEDF+ER+AKRTLSRNWFV
Sbjct: 121 YHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLSRNWFV 180

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
           GFLKYGRPKNVEDLQK+GFYRFLRKTYLLHPIALA+VLY FGGLPFVVWGMGVRLICVLH
Sbjct: 181 GFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRLICVLH 240

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
           AA FVNSACHIWGYRKWNTNDLSKNNW+IGL+S GEGWHNNHHAFEYSARHGLQWWEFDF
Sbjct: 241 AALFVNSACHIWGYRKWNTNDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQWWEFDF 300

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQDKITSP 338
           TWWVILLLQALGLATDVKLP+Q H QKLAIQ KITSP
Sbjct: 301 TWWVILLLQALGLATDVKLPTQLHKQKLAIQYKITSP 337

BLAST of CmoCh14G013890 vs. NCBI nr
Match: XP_022153467.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 575.9 bits (1483), Expect = 2.3e-160
Identity = 262/332 (78.92%), Postives = 290/332 (87.35%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           MEATRKEVED+ DKK  FSVV P+RP L RKWT LDRR+A  ILFLH+L L APFH NW 
Sbjct: 1   MEATRKEVEDQKDKKVAFSVVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPFHCNWA 60

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           AFWVAF LYF TG+ GITLSYHRNLAH+SFKLPKWLEY+FAYLGV AFQGDPIDWVSTHR
Sbjct: 61  AFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDWVSTHR 120

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHH+FVDTERDPHSPLEGFWFSH+ WMFD+Y LT K+ PK ++DF+ERDAKR    N FV
Sbjct: 121 YHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRFSNIFV 180

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
           GF+KYGRP NVEDLQK+ FYRFLRKTY+LHP ALA+VLY  GGLPF+VWGMGVRL+ VLH
Sbjct: 181 GFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRLVWVLH 240

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
             FFVNSACHIWGYR+W+TNDLSKNNW IGL++ GEGWHNNHHAFEYSARHGLQWWEFD 
Sbjct: 241 ITFFVNSACHIWGYRRWDTNDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQWWEFDL 300

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAIQD 333
           TW V+L+LQALGLATDVKLPSQ H QKLA+ +
Sbjct: 301 TWCVVLVLQALGLATDVKLPSQIHKQKLALHN 332

BLAST of CmoCh14G013890 vs. NCBI nr
Match: XP_022153442.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 558.9 bits (1439), Expect = 3.0e-155
Identity = 257/337 (76.26%), Postives = 285/337 (84.57%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKK-PFS---VVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFH 60
           MEATRKE+EDK + KK  FS   V+ P+R FL RKWT LDRR+A GILFLH+L L APFH
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIVLKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPFH 60

Query: 61  YNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWV 120
            NW AFWVAF LYF TG+ GITLSYHRNLAH+SFKLPKWLEY FAYLG+ AFQGDPIDWV
Sbjct: 61  CNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWV 120

Query: 121 STHRYHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSR 180
           STHRYHHQFVDTERDPHSPLEGFWFSH+TWMFD+Y LT K+ PK +EDFEE+DAKR    
Sbjct: 121 STHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFS 180

Query: 181 NWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLI 240
           N FV  +KYGRP NV+DLQK+ FYRFLR+TY+LHP ALA+VLY  GGLPFVVWGMGVRL+
Sbjct: 181 NIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRLV 240

Query: 241 CVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWW 300
             LH  FF+NSACHIWGYR+WNT DLSKNNWV GL++ GEGWHNNHHAFEYSARHGLQWW
Sbjct: 241 WFLHITFFINSACHIWGYRRWNTTDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWW 300

Query: 301 EFDFTWWVILLLQALGLATDVKLPSQRHMQKLAIQDK 334
           EFD TWWV+L LQ +GLATDVKLPSQ   QKLA+  K
Sbjct: 301 EFDLTWWVVLFLQTIGLATDVKLPSQGQKQKLAMDHK 337

BLAST of CmoCh14G013890 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 396.4 bits (1017), Expect = 2.4e-110
Identity = 175/300 (58.33%), Postives = 217/300 (72.33%), Query Frame = 0

Query: 30  RKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRS 89
           R+W  +D    A +L +H+L L APF +NW A  VAF LY  TG+LGITLS+HRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 90  FKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHVTWMFD 149
           FKLPKWLEY FAY G QA QG+PIDWVSTHRYHHQF D++RDPHSPL+GFWFSH+ WMFD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 150 SYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLL 209
           +  +T +                             G P NV DL+K+ FYRFLR TY+L
Sbjct: 216 TNTITQRC----------------------------GEPNNVGDLEKQPFYRFLRTTYIL 275

Query: 210 HPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVI 269
           HP+ALA+ LY  GG PF+VWGMGVR++ V H  + VNSACH+WG + WNT DLSKNNW +
Sbjct: 276 HPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWV 335

Query: 270 GLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKLPSQRHMQKLA 329
             ++ GEGWHNNHHAFE+SARHGL+WW+ D TW+V+  LQA+GLATDVKLPS+   Q++A
Sbjct: 336 AALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of CmoCh14G013890 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 347.8 bits (891), Expect = 9.6e-96
Identity = 159/311 (51.13%), Postives = 206/311 (66.24%), Query Frame = 0

Query: 20  VVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGITL 79
           VV  KR F  R WT+ D       +  H+L L APF+++W AFWV   L F  G+  ITL
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 80  SYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGF 139
           SYHRNL+HRSF LPKWLEY FAY GV AFQGDPI+WVS HRYHH+  +T+RDPHSP +GF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 140 WFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEGF 199
           WFSH+ W+FD+  +                                G  +NV+DL ++ F
Sbjct: 199 WFSHMAWIFDTSSILENC----------------------------GGEENVDDLVRQPF 258

Query: 200 YRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWNT 259
           YRFL++T LLH +A + + Y  GG+P +VWG+G+ +   LH  F VNS CHIWG R WNT
Sbjct: 259 YRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNT 318

Query: 260 NDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVKL 319
           +D SKNNW + +++ GEGWHNNHHAFE+SARHGL+WW+ D TW +I  L+A+GLAT+VKL
Sbjct: 319 SDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKL 360

Query: 320 PSQRHMQKLAI 331
           P++  M+  A+
Sbjct: 379 PTETQMKGKAL 360

BLAST of CmoCh14G013890 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 345.9 bits (886), Expect = 3.7e-95
Identity = 163/330 (49.39%), Postives = 209/330 (63.33%), Query Frame = 0

Query: 1   MEATRKEVEDKYDKKKPFSVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWP 60
           + A+ KE  +K        +   KR    RKW  LD   A   LF+H L L APF++ WP
Sbjct: 3   LSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWP 62

Query: 61  AFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHR 120
           A  VA  +Y T G LGIT+SYHRNLAHRSFK+PKWLEY FAY G+ A QGDPIDWVSTHR
Sbjct: 63  ALRVALIVY-TVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHR 122

Query: 121 YHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFV 180
           YHHQF D++RDPHSP EGFWFSH+ W+FD+  L  K                        
Sbjct: 123 YHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------- 182

Query: 181 GFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLH 240
                GR  NVEDL+++ +Y+FL++T L H +    +LY FGGL F+ WGMG+ +    H
Sbjct: 183 -----GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHH 242

Query: 241 AAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDF 300
               +NS CH+WG R W TND S+N W + + S GE WHNNHHAFE SAR GL+WW+ D 
Sbjct: 243 VTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDI 302

Query: 301 TWWVILLLQALGLATDVKLPSQRHMQKLAI 331
           +W+++  L+ +GLATDVKLPS+   +++A+
Sbjct: 303 SWYIVRFLEIIGLATDVKLPSESQRRRMAM 303

BLAST of CmoCh14G013890 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 339.0 bits (868), Expect = 4.5e-93
Identity = 161/328 (49.09%), Postives = 207/328 (63.11%), Query Frame = 0

Query: 8   VEDKYDK--KKPFSVVNPKR---PFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAF 67
           VE+ + K    P +V   K+    F +R+W  LD    +    +H L L APF++ W A 
Sbjct: 7   VEENHQKNPSTPAAVEEKKKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSAL 66

Query: 68  WVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYH 127
           WV F L++T G LGIT+SYHRNLAHRSFK+PKWLEY  AY  + A QGDPIDWVSTHRYH
Sbjct: 67  WVTF-LFYTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYH 126

Query: 128 HQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGF 187
           HQF D+ERDPHSP EGFWFSH+ W++DS  L +K                          
Sbjct: 127 HQFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC------------------------- 186

Query: 188 LKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAA 247
              GR  NVEDL+++ FYRFL+KT L H + L   L+  GG+ FV WGMGV     +H  
Sbjct: 187 ---GRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVT 246

Query: 248 FFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTW 307
             +NS CHIWG R W TND S+N W + + S GE WHNNHHAFE SAR GL+WW+ D +W
Sbjct: 247 CLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISW 305

Query: 308 WVILLLQALGLATDVKLPSQRHMQKLAI 331
           +++   + +GLATDVK+P++   +++AI
Sbjct: 307 YIVRFFEIIGLATDVKVPTEAQRRRMAI 305

BLAST of CmoCh14G013890 vs. TAIR 10
Match: AT1G06360.1 (Fatty acid desaturase family protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.1e-88
Identity = 160/312 (51.28%), Postives = 196/312 (62.82%), Query Frame = 0

Query: 19  SVVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPFHYNWPAFWVAFTLYFTTGVLGIT 78
           +V   KRP+++R+W+  D   A  ++ +H L L APF+Y W A    F LY  T  L IT
Sbjct: 15  AVSKEKRPYIHREWSWADIIRALTVINVHFLCLLAPFNYKWEALRFGFVLYALTS-LSIT 74

Query: 79  LSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEG 138
            SYHRNLAHRSFKLPKWLEY  AY  V A QGDP+DWVS HR+HHQF D++RDPHSP+EG
Sbjct: 75  FSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEG 134

Query: 139 FWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLSRNWFVGFLKYGRPKNVEDLQKEG 198
           FWFSHV W+ D+       Y KY                      K G   NV DL+++ 
Sbjct: 135 FWFSHVWWICDTR------YIKY----------------------KCGGRNNVMDLKQQW 194

Query: 199 FYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRLICVLHAAFFVNSACHIWGYRKWN 258
           FY FLR T   H +    VLY +GGLP++  G GV  +   H  + VNSACHIWG R W 
Sbjct: 195 FYWFLRMTIGFHVLMFWTVLYLYGGLPYLTCGGGVGGVIGYHVTWLVNSACHIWGSRSWK 254

Query: 259 TNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQWWEFDFTWWVILLLQALGLATDVK 318
           T D S+N W + L + GE WHNNHHAFE SAR GL+WW+ D TW++I L + LGLATDVK
Sbjct: 255 TKDTSRNVWWLSLFTMGESWHNNHHAFESSARQGLEWWQIDITWYLIRLFEVLGLATDVK 297

Query: 319 LPSQRHMQKLAI 331
           LPS+   QKLA+
Sbjct: 315 LPSEIQKQKLAL 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X03.3e-10958.33Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
P0DOW21.4e-9653.47Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
P0DOW39.4e-9653.25Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Q9FV682.7e-9554.13Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
Q9LVZ31.4e-9451.13Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GVF31.4e-206100.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A0A0KWV41.5e-18489.02Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1[more]
A0A6J1DIZ71.1e-16078.92palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIX01.4e-15576.26palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GCE16.6e-15377.81palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
XP_022955953.12.8e-206100.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_023528301.15.3e-20598.81palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_004152341.23.0e-18489.02palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_022153467.12.3e-16078.92palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022153442.13.0e-15576.26palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
Match NameE-valueIdentityDescription
AT3G15850.12.4e-11058.33fatty acid desaturase 5 [more]
AT3G15870.19.6e-9651.13Fatty acid desaturase family protein [more]
AT1G06080.13.7e-9549.39delta 9 desaturase 1 [more]
AT2G31360.14.5e-9349.0916:0delta9 desaturase 2 [more]
AT1G06360.11.1e-8851.28Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 119..148
score: 51.05
coord: 59..81
score: 24.71
coord: 82..102
score: 33.33
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 21..330
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 59..318
e-value: 2.21198E-75
score: 227.824
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 58..304
e-value: 2.1E-17
score: 63.8
NoneNo IPR availablePANTHERPTHR11351:SF87FATTY ACID DESATURASE, TYPE 1, CORE-RELATEDcoord: 21..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh14G013890.1CmoCh14G013890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042149 cellular response to glucose starvation
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031588 nucleotide-activated protein kinase complex
cellular_component GO:0005634 nucleus
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016208 AMP binding
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function GO:0019901 protein kinase binding
molecular_function GO:0019887 protein kinase regulator activity