CmoCh14G012300 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh14G012300
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	(S)-2-hydroxy-acid oxidase
Location	Cmo_Chr14: 10442377 .. 10447535 (-)
RNA-Seq Expression	CmoCh14G012300
Synteny	CmoCh14G012300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

NNNNNNNNNTACAAAAAAAAAAAAAAAAAAAAAAACACAGAAAGAGGTCTCTACACATCTGGCCTCATCTCTTTCTTCCCCTCTGTGAATTAGGTTTTCTGTCTCTTTATTTTTTTTTGGCGTAATCTAGGTATGTCAAGTTTTCGCTCCCAATCATATTCAAATTGTTTCTTGTTATGAGACAATTTGACTAACTTGCTGAATGGTGTTTCAAATTATATCAAAGTTATGTAGGTGTTAAATCAAAGATGGTGAAGCTGATAATGATTGCCTATGTTCTGACGGTCTTCCTCATGCTGATGGACTAGATGATGGTCGTGATTTGAGAGATGCTGAGCAGCAACCAAAGCGTTGTTTAAGAAATTATCAAGAGGACAAAATGAGCCTTCGAGGATGTCTATTGAAACTGGCTCTTATGTCCTTCAGTATCCTCGCATCACGCAAGTTTGCATTCCATGCGCAATGAAATCACATAAGTTATATTCTTGTCATGCATCATTAGGCCACCATTTAGGCAATACCAAGATCATAGTCTAAAGCAGATTGTGGACTTGACCATTAGGCCACCATTTAGGCAATACCAAGATCATAGTCTAAAGCAGATTGTGGACTTGACACCAAACCATAATGTATGGTTGGAACTGAAACATTCTTGAACTATTGCAGTTGGTTCCATTTCTTCCCATATTTATTGGCAGTGTTCATACTATTGTCTTAGAAGTGTCTGGATTTTATGTTTGCTTGACAGCATTCAGTTATATAATTGAAGGGCGTGTTTGCTAATTGACAATTTGTGACCATGCTTACCCAAAAAAACTTGCTTTCCAGTACCTTGAAGACCTGAAGAATGAATTTGAGCGTGTTAATAGGGTTCAAATTGAAACAGCTGCTAGACCCAGAATCTACTCATTTGCCTCCTTTTTATGAATTGGTTATGGAAGAATTAATCTTGCCACATCTCTGGAAAGCAACAGAATTGATTTGTGGTGCGATTGATAAATGATAACGGGCCTTAATGTTTGGACTACACATCTGTTTTGTTTGGCCCATACTTGCACTGCTCTATTTGATAACGAGGAATAGCACACTGATCTGTCTGATAATGAGGAATAGCTGGCCTCCACATTGGGATCATCAACAAAGTTGTCAACATTAGAATATTAGCCACATCACCCATCGGCATCGAACGTTACTATCGGTACTGATTATGAATTTAAGATAAACCAGAGTAGATAGGAGGTACCGACAAGTTTACAATGCATCGGTTTAAATTATTTGATAGATATTTTCTATGCTACACGCATTCATTAGCAAAATTTCTGTCGTCAACAACTTGAACTTCTTGACCCATTCTCCACTGAAGGCTGCACCTTCGAGCTTGGGTCAAAATTACAACATCAAGGTAAAATCTGATTCCAGTCTTTTAAATTGTTTGCGGCAATCATCATATTTTTCTGTTTTCCATGAAAGTGACGAAGTGTTATTCCTTAGATTCCATCTTTAAGCTTTACTTTCTGCTGATCTACTGCTTTCTTATGTAAGTTTTCGTTAAGATTACTGAGAAAAAAGGATCGTGGGACATCTTGCTTTTCCCTGGTTGTGATGCCTTTGTAATGTTTATTGCTTCAGTTGTACTTGTCAAGAAAGTCACCCTCTAAGCTAATTATAAATTAATATTTCTGTGTGTGTGTACTGCTAATGTGTCATGTTCCACACATGAAGTTTGGCTAACCAGTATGTTGGTACTGTATGTTCCAGTGGGGAAACGGGGAAGCTTCGCATCTCCCCCTCCTCAATAAGTAGATTAAATCGTAGTAGAGAAAATAAAGTTGCATTATCATATAAGATATTCCTTCCATTTTTTTTAGTTTTTGTTCCAAAAGAAGATTAAAGAACACAATTCAATGGTCGAACTTTTGGGCAAGACCTTCCCATATTCTTCAACTGTTCGAACAACATATTGATGCATCTTCAGTCCTTACTAGAGGATTACAGACCAAATCTTAAATCAACCATCGTCGCTATGCCTTTTGTTCTGATTCACTTTACTCTTGCTTGTTTACTGGGTGTTGTGGGGAACTCGCTCTTGTCTCGTTTATTTGGATGCATCATCCTAAAGTCATGGTACCATAAGTCTCTTATTATAAATTTTAAGCGAGTTGAGACTCTAATTTCAATCTACTGTGCCTCAAGACCTTTCATGGCTACTGGTTCTTTGGGTGGCATCTGTTACTCTTTTCCTAACAAATATAAGTATCAATCACTCAAGGATGTTCTGGTCTCTTTTTTGTCAGGACAAAATGTCTTCTGAACCAGTGAACGTGAATGACTTCAAAGAATTGGCTAGGTTGAAACTTCCAAAAATGTACTATGATTTCTACGCAGGAGGGGCTGAGGACGAACACACACTCAGAGAGAACATACAAGCTTTCTGTAGAATCATGTAATTTTGAAGCTAACTAACGATTGCTTTGAAAAAGTCATGCTATAAGTTATTCATGGTTTCTATGGTTTTCAGGATTCAACCTCGAGTTCTCGTGGACGTGAGCCAAATTGATATGTCAACAACCATATTGGGCCATCGCATCTCCGCACCCATCCTGGTTGCCCCCACTGCTGCACATAAGTTGGCATTCCATGAAGGTAAACCTCTTGGTATATTGCTTAGTTGAGAATTGATTTTCATTCTACTTTTTCTTGATGATATCTTTGATCTTTCAGGAGAACAAGCCACGGCCAGGGCAGCTGCTGCTGCAAAAACTATAATGGTACTAATGTGACTACAAGCTATAAGTTATTTTACTTGCTGAATTTGCTAATTGAGTTAAGTTGCTTTTGTTTGTAGATTCTGTCTTACTCTTCGACCTGTTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTCCGTTTCTTTCAATTATATGTAAATGACGCTTCTTTTGCACATTTTTCTATCCCTTTTTCAATTCTTTCCTATAGCAGCAGTCCAGTTATTCGCAAGTCTTAATCTTAGTTTTTTTATTCTGTAATCACCAGATTTTCAAACGGCGCGATATCTCGGCTCTGCTAGTTCAGAGAGCTGAGAGATTTGGATACAAGGCAATTGTTCTGACTGCCGATACTCCTCGACTTGGTAGAAGGGAGGCTGACATAAAGAACAAGTAGCAATATTTCATTTCAGTTTTTTTCCATAGAACCTTCTTAATACCTTTATCTTGCATCCCAATTGTAAGCTATGCCTTGGTTTTTGTCTCATCTGGAAAAACACTTCGTTAAATTTGTAGGATGATTGCACCACCAGTGAAGAATCTTGAAGGCCTCATATCTATTGACGTTAACTCTGTGAGTATTATTCTGATAAAATCCGGTTTAGAATATTTGAATTACCACTTACAAATACAATGGAGGAATTTTTGAATCTTGCTTGATCAGTAATCTGGGATGTGCATACATCCAGGTTTCCTTTTACTTTCCCTCAAGCATACTTCCATGTATCTTAATTCATATCAAATTATAAAACCTCATTTCTATGCCCTAGGCTTACTTGCGACTCATGATCTTATGTAGGATCAAGGTTCAAAGTTAGAAACTTATGCCAACGAGATGTTGGATGCATCTCTCCGCTGGGAAGTATGTCCAATAATCTGCTCAGATTCTTTGTATGTCTATTTTCTTTCCAGTTTAGTTATAAAATTTTACCATCTACAGGATATAAGCTGGTTAAGATCAATCACTAGTCTGCCAATTCTGATAAAGGGGATTCTCACTCATGAAGATGGTAAGGTTCAGTTATGTCTTAACGATATTAGAATCAAGAAATGAGCCTTCTTTTTATCTCCTGGGTTTATGAGTTGTGTTACTGTCAATTGATTAGTTCTCGAACATGTTTGATGAATGGCAGCAACTAAAGCTGTGGAAGCTGGTGTAGATGGAATTATTGTCTCCAATCATGGAGCCCGCCAACTAGACTTTGCTCCTGCCACTATTTCTGTCCTTGAAGAGGTAAGTTTCGTTGTGTACTACAATATTAGAATTAATTTCACATGACTTGAACTAGATTAGAATGAGAGTCAAGCTTATCAGATATCAAGTGTAGTTGAAAAGTAATATCCATCATCTATACGGTTGCTTTACTTATAACTGTAATAATCTTTCATCAGTTGGGTTTTTTAACAGGAAGTTTGATGCAGGTTGTTCATGCTGTCAAGGGTAAAATTCCTGTGCTCTTAGATGGAGGTGTGCGGCGGGGTACAGATGTGTTTAAGGCATTGGCCCTCGGGGCCCAGGCAGTCCTTGTAAGTTCTATACCACCAACTAAAACATTACATATTTTTTGGTTAAATCCCTAAACTTTAAGATTTGTATCTGATTAATCCATGAACTTGTATATGTATGATAAAGTTCTTGATGTATTCAACATTGTTGTAAAATTCACGAACGTACTATTAAACACGAACTTAAAAATTTAAGGCTCATTCGAACATAACATTCAATTTTTATATTTATTAGATAAGTTAATCTTTATTAATTTGAATATATTAGGGACATGTTAAATACTTAATTGAAAGTTGGGACCTATTTGACATTTTTTTTAAAGTTGAAGAACATATTAGACATAGAATTAAAATTTGGGTGATCTATTAGATACTTTTTAAGTTAGAGACTGTATTTTATCTGTAGTTTCATTCCAAGTTACAACAGGATTACACTTGTATTCACACATAAAAGGTCTCCAATGTTAGATCTGTGCTAAATGATGTTGGATAAAATCCTGGGGTTCTTGGTTTCAACTTATAATTGAATTATGTTTCTTGTTTCTTGTCTTTGATTACGGGCATGGCTCTCCCCTCTCATGTTAGAAATGGTTGTGATTTGATTCAGATAGGGAGACCAGTTTTATTTGGACTGGCAGCAAAGGGAGAAGGAGGAGTAAGAACAGTGCTGGAGATGCTGAAGAATGAGTTAGAGATTAGTATGGCACTTTCTGGGTGCCCATGCATCAAGGACATTACTAGAAGCCATGTTAGGACACCTTACGATAAGCTGCCTTCAATGCTTTAGTGCCTCTGTATACCATTTATATATATAATTTAAGCATAGCTGAGTTAGTCTTACAAATAAGAGGAAGCTGGGAACTCTACAAAAAAAAAAANNNNNNN

mRNA sequence

NNNNNNNNNTACAAAAAAAAAAAAAAAAAAAAAAACACAGAAAGAGGTCTCTACACATCTGGCCTCATCTCTTTCTTCCCCTCTGTGAATTAGGTTTTCTGTCTCTTTATTTTTTTTTGGCGTAATCTAGGTATGTCAAGTTTTCGCTCCCAATCATATTCAAATTGTTTCTTGTTATGAGACAATTTGACTAACTTGCTGAATGGTGTTTCAAATTATATCAAAGTTATGTAGGTGTTAAATCAAAGATGGTGAAGCTGATAATGATTGCCTATGTTCTGACGGTCTTCCTCATGCTGATGGACTAGATGATGGTCGTGATTTGAGAGATGCTGAGCAGCAACCAAAGCGTTGTTTAAGAAATTATCAAGAGGACAAAATGAGCCTTCGAGGATGTCTATTGAAACTGGCTCTTATGTCCTTCAGTATCCTCGCATCACGCAAGTTTGCATTCCATGCGCAATGAAATCACATAAGTTATATTCTTGTCATGCATCATTAGGCCACCATTTAGGCAATACCAAGATCATAGTCTAAAGCAGATTGTGGACTTGACCATTAGGCCACCATTTAGGCAATACCAAGATCATAGTCTAAAGCAGATTGTGGACTTGACACCAAACCATAATGTATGGTTGGAACTGAAACATTCTTGAACTATTGCAGTTGGTTCCATTTCTTCCCATATTTATTGGCAGTGTTCATACTATTGTCTTAGAAGTGTCTGGATTTTATGTTTGCTTGACAGCATTCAGTTATATAATTGAAGGGCGTGTTTGCTAATTGACAATTTGTGACCATGCTTACCCAAAAAAACTTGCTTTCCAGTACCTTGAAGACCTGAAGAATGAATTTGAGCGTGTTAATAGGGTTCAAATTGAAACAGCTGCTAGACCCAGAATCTACTCATTTGCCTCCTTTTTATGAATTGGTTATGGAAGAATTAATCTTGCCACATCTCTGGAAAGCAACAGAATTGATTTGTGGTGCGATTGATAAATGATAACGGGCCTTAATGTTTGGACTACACATCTGTTTTGTTTGGCCCATACTTGCACTGCTCTATTTGATAACGAGGAATAGCACACTGATCTGTCTGATAATGAGGAATAGCTGGCCTCCACATTGGGATCATCAACAAAGTTGTCAACATTAGAATATTAGCCACATCACCCATCGGCATCGAACGTTACTATCGGTACTGATTATGAATTTAAGATAAACCAGAGTAGATAGGAGGTACCGACAAGTTTACAATGCATCGGTTTAAATTATTTGATAGATATTTTCTATGCTACACGCATTCATTAGCAAAATTTCTGTCGTCAACAACTTGAACTTCTTGACCCATTCTCCACTGAAGGCTGCACCTTCGAGCTTGGGTCAAAATTACAACATCAAGGACAAAATGTCTTCTGAACCAGTGAACGTGAATGACTTCAAAGAATTGGCTAGGTTGAAACTTCCAAAAATGTACTATGATTTCTACGCAGGAGGGGCTGAGGACGAACACACACTCAGAGAGAACATACAAGCTTTCTGTAGAATCATGATTCAACCTCGAGTTCTCGTGGACGTGAGCCAAATTGATATGTCAACAACCATATTGGGCCATCGCATCTCCGCACCCATCCTGGTTGCCCCCACTGCTGCACATAAGTTGGCATTCCATGAAGGAGAACAAGCCACGGCCAGGGCAGCTGCTGCTGCAAAAACTATAATGATTCTGTCTTACTCTTCGACCTGTTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTCCGTTTCTTTCAATTATATATTTTCAAACGGCGCGATATCTCGGCTCTGCTAGTTCAGAGAGCTGAGAGATTTGGATACAAGGCAATTGTTCTGACTGCCGATACTCCTCGACTTGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTGCACCACCAGTGAAGAATCTTGAAGGCCTCATATCTATTGACGTTAACTCTGATCAAGGTTCAAAGTTAGAAACTTATGCCAACGAGATGTTGGATGCATCTCTCCGCTGGGAAGATATAAGCTGGTTAAGATCAATCACTAGTCTGCCAATTCTGATAAAGGGGATTCTCACTCATGAAGATGCAACTAAAGCTGTGGAAGCTGGTGTAGATGGAATTATTGTCTCCAATCATGGAGCCCGCCAACTAGACTTTGCTCCTGCCACTATTTCTGTCCTTGAAGAGGTTGTTCATGCTGTCAAGGGTAAAATTCCTGTGCTCTTAGATGGAGGTGTGCGGCGGGGTACAGATGTGTTTAAGGCATTGGCCCTCGGGGCCCAGGCAGTCCTTATAGGGAGACCAGTTTTATTTGGACTGGCAGCAAAGGGAGAAGGAGGAGTAAGAACAGTGCTGGAGATGCTGAAGAATGAGTTAGAGATTAGTATGGCACTTTCTGGGTGCCCATGCATCAAGGACATTACTAGAAGCCATGTTAGGACACCTTACGATAAGCTGCCTTCAATGCTTTAGTGCCTCTGTATACCATTTATATATATAATTTAAGCATAGCTGAGTTAGTCTTACAAATAAGAGGAAGCTGGGAACTCTACAAAAAAAAAAANNNNNNN

Coding sequence (CDS)

ATGCTACACGCATTCATTAGCAAAATTTCTGTCGTCAACAACTTGAACTTCTTGACCCATTCTCCACTGAAGGCTGCACCTTCGAGCTTGGGTCAAAATTACAACATCAAGGACAAAATGTCTTCTGAACCAGTGAACGTGAATGACTTCAAAGAATTGGCTAGGTTGAAACTTCCAAAAATGTACTATGATTTCTACGCAGGAGGGGCTGAGGACGAACACACACTCAGAGAGAACATACAAGCTTTCTGTAGAATCATGATTCAACCTCGAGTTCTCGTGGACGTGAGCCAAATTGATATGTCAACAACCATATTGGGCCATCGCATCTCCGCACCCATCCTGGTTGCCCCCACTGCTGCACATAAGTTGGCATTCCATGAAGGAGAACAAGCCACGGCCAGGGCAGCTGCTGCTGCAAAAACTATAATGATTCTGTCTTACTCTTCGACCTGTTCAATAGAGGAAGTTGCCTCTAGTTGCAATGCTGTCCGTTTCTTTCAATTATATATTTTCAAACGGCGCGATATCTCGGCTCTGCTAGTTCAGAGAGCTGAGAGATTTGGATACAAGGCAATTGTTCTGACTGCCGATACTCCTCGACTTGGTAGAAGGGAGGCTGACATAAAGAACAAGATGATTGCACCACCAGTGAAGAATCTTGAAGGCCTCATATCTATTGACGTTAACTCTGATCAAGGTTCAAAGTTAGAAACTTATGCCAACGAGATGTTGGATGCATCTCTCCGCTGGGAAGATATAAGCTGGTTAAGATCAATCACTAGTCTGCCAATTCTGATAAAGGGGATTCTCACTCATGAAGATGCAACTAAAGCTGTGGAAGCTGGTGTAGATGGAATTATTGTCTCCAATCATGGAGCCCGCCAACTAGACTTTGCTCCTGCCACTATTTCTGTCCTTGAAGAGGTTGTTCATGCTGTCAAGGGTAAAATTCCTGTGCTCTTAGATGGAGGTGTGCGGCGGGGTACAGATGTGTTTAAGGCATTGGCCCTCGGGGCCCAGGCAGTCCTTATAGGGAGACCAGTTTTATTTGGACTGGCAGCAAAGGGAGAAGGAGGAGTAAGAACAGTGCTGGAGATGCTGAAGAATGAGTTAGAGATTAGTATGGCACTTTCTGGGTGCCCATGCATCAAGGACATTACTAGAAGCCATGTTAGGACACCTTACGATAAGCTGCCTTCAATGCTTTAG

Protein sequence

MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML

Homology

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BLAST of CmoCh14G012300 vs. ExPASy Swiss-Prot
Match: Q9LJH5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=GLO4 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.0e-149
Identity = 261/359 (72.70%), Postives = 316/359 (88.02%), Query Frame = 0


Query: 45  VNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTT 104
           VNV++F+ELA+  LPKMYYDFY GGAED+HTL EN+QAF RIM +PRVLVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 105 ILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAV 164
           +LG+ ISAPI++APTA HKLA  +GE ATA+AAAA  TIMI+S+ STC+IEEVASSCNAV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 165 RFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGL 224
           RF Q+Y++KRRD++A +V+RAE+ G+KAIVLT D PRLGRREADIKNKMI+P +KN EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 225 ISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGV 284
           +S +V  ++GS +E +A+   DASL W+DI WLRSIT LPIL+KG+LT EDA KAVEAGV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 285 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 344
           DGI+VSNHGARQLD++PATI+VLEEVVHAVKG+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 345 IGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           IGRP+++GLAAKGE GV+ V++MLKNE EI+MALSGCP I D+TR+HVRT  +++ SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTENERIKSML 363

[Close]

BLAST of CmoCh14G012300 vs. ExPASy Swiss-Prot
Match: Q24JJ8 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=GLO3 PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.4e-146
Identity = 259/359 (72.14%), Postives = 308/359 (85.79%), Query Frame = 0


Query: 45  VNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTT 104
           VNV++F+ELA+  LPKMYYDFY GGAED+HTL EN+QAF RIM +PRVLVDVS+IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 105 ILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAV 164
           ILG+ ISAPI++APT  HKLA  EGE ATA+AAAA  TIMI+SY S+C+ EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 165 RFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGL 224
           RF Q+Y++KRRDI+A +V+RAE+ G+KAIVLT D PRLGRREADIKNKMI+P +KN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 225 ISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGV 284
            S +V   +GS ++ +A+   DAS  W+DI WLRSIT LPIL+KGILT EDA KAVEAGV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 285 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 344
           DGIIVSNHG RQLD++PATI+VLEEVV  V+G+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 345 IGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           IGRP+++GLAAKGE GV+ V++MLKNE EI+MALSGCP I DITR+HVRT  ++L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTENERLHSML 363

[Close]

BLAST of CmoCh14G012300 vs. ExPASy Swiss-Prot
Match: Q8H3I4 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO4 PE=1 SV=2)

HSP 1 Score: 476.5 bits (1225), Expect = 3.0e-133
Identity = 236/361 (65.37%), Postives = 299/361 (82.83%), Query Frame = 0


Query: 44  PVNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMST 103
           PVNV +++ELA+  LPKM YD+  GGAEDEHTLRENI A+ RI+++PRVLVDVS+IDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 104 TILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNA 163
           T+LG+ + +PI+VAPT  HKLA  EGE+ATARAAA+   IM+LS+SS+C IE+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 164 VRFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEG 223
           +RF+QLY++K R++SA LV+RAE  G+KA++LT DTP LGRREADI+NKM+ P   NLEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 224 LISI-DVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEA 283
           L++  D ++  GS+LE +A   LD SL W+DI WL+SITS+PI +KGI+T EDA +AVEA
Sbjct: 186 LMTTDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 284 GVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQA 343
           GV G+IVSNHGARQLD+APATI+ LEEVV AV G +PVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 344 VLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSM 403
           V++GRPV FGLAA+GE G R V+EML  ELE++MAL GC  + +ITRSHV T  D++ S+
Sbjct: 306 VMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGDRIRSL 365

[Close]

BLAST of CmoCh14G012300 vs. ExPASy Swiss-Prot
Match: B8B8K5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=39946 GN=GLO4 PE=3 SV=2)

HSP 1 Score: 471.9 bits (1213), Expect = 7.4e-132
Identity = 235/361 (65.10%), Postives = 297/361 (82.27%), Query Frame = 0


Query: 44  PVNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMST 103
           PVNV +++ELA+  LPKM YD+  GGAEDEHTLRENI A+ RI+++PRVLVDVS+IDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 104 TILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNA 163
           T+LG+ + +PI+VAPT  HKLA  EGE+ATARAAA+   IM+LS+SS+C IE+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 164 VRFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEG 223
           +RF+QLY++K R++SA LV+RAE  G+KA++LT DTP LGRREADI+NKM+ P   NLEG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 224 LISI-DVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEA 283
           L++I D ++  GS+LE +A   LD SL W+DI WL+SITS+PI +KGI+T EDA +AVEA
Sbjct: 186 LMTIDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEA 245

Query: 284 GVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQA 343
           GV G+IVSNHGARQLD+APATI+ LEEVV AV G +PVL+DGG+RRGTDVFKALALGA+A
Sbjct: 246 GVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARA 305

Query: 344 VLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSM 403
           V+   PV FGLAA+GE G R V+EML  ELE++MAL GC  + +ITRSHV T  D++ S+
Sbjct: 306 VMXXXPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVMTEGDRIRSL 365

[Close]

BLAST of CmoCh14G012300 vs. ExPASy Swiss-Prot
Match: B8AKX6 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 OS=Oryza sativa subsp. indica OX=39946 GN=GLO1 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 4.2e-119
Identity = 218/360 (60.56%), Postives = 277/360 (76.94%), Query Frame = 0


Query: 43  EPVNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMS 102
           E  NV +++ +A+ KLPKM YD+YA GAEDE TL+EN +AF RI+ +PR+L+DVS+IDMS
Sbjct: 3   EITNVMEYQAIAKQKLPKMIYDYYASGAEDEWTLKENREAFSRILFRPRILIDVSKIDMS 62

Query: 103 TTILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCN 162
            T+LG +IS PI++AP+A  K+A  +GE ATARAA+AA TIM LS  +T S+EEVAS+  
Sbjct: 63  ATVLGFKISMPIMIAPSAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 122

Query: 163 AVRFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPP---VK 222
            +RFFQLY++K R++   LV+RAER G+KAI LT DTPRLGRREADIKN+ + PP   +K
Sbjct: 123 GIRFFQLYVYKDRNVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPYLTLK 182

Query: 223 NLEGLISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKA 282
           N EGL   +++    S L +Y    +D +L W+D+ WL+SITSLPIL+KG++T EDA  A
Sbjct: 183 NFEGLDLAEMDKSNDSGLASYVAGQIDRTLSWKDVKWLQSITSLPILVKGVITAEDARLA 242

Query: 283 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 342
           V +G  GIIVSNHGARQLD+ PATIS LEEVV A  G+IPV LDGGVRRGTDVFKALALG
Sbjct: 243 VHSGAAGIIVSNHGARQLDYVPATISALEEVVTAAAGRIPVYLDGGVRRGTDVFKALALG 302

Query: 343 AQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKL 400
           A  V IGRPV+F LAA+GE GVR VL M++ E E++MALSGC  + DITR+H+ T  D+L
Sbjct: 303 AAGVFIGRPVVFALAAEGEAGVRNVLRMMREEFELTMALSGCTSLADITRAHIYTDADRL 362

[Close]

BLAST of CmoCh14G012300 vs. ExPASy TrEMBL
Match: A0A6J1GUB4 ((S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 6.2e-222
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
           AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL
Sbjct: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
           ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA
Sbjct: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 403

[Close]

BLAST of CmoCh14G012300 vs. ExPASy TrEMBL
Match: A0A6J1IRU2 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479940 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.5e-212
Identity = 388/403 (96.28%), Postives = 392/403 (97.27%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVN LNF THSPLKAAPSSLGQNYNIK KMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MLHAFISKISVVNYLNFSTHSPLKAAPSSLGQNYNIKGKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMI+PRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIRPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
            HKLAFHEGE ATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL
Sbjct: 121 GHKLAFHEGELATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPP+KNLEGL+SIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPMKNLEGLMSIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
           ANE LDASL WEDI WLRS TSLPILIKGILTHEDATKAVE GVDGIIVSNHGARQLDFA
Sbjct: 241 ANETLDASLSWEDIMWLRSTTSLPILIKGILTHEDATKAVEGGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRT +DKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTQHDKLPSML 403

[Close]

BLAST of CmoCh14G012300 vs. ExPASy TrEMBL
Match: A0A6J1IU35 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479940 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.0e-208
Identity = 388/429 (90.44%), Postives = 392/429 (91.38%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVN LNF THSPLKAAPSSLGQNYNIK KMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MLHAFISKISVVNYLNFSTHSPLKAAPSSLGQNYNIKGKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMI+PRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIRPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
            HKLAFHEGE ATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL
Sbjct: 121 GHKLAFHEGELATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPP+KNLEGL+SIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPMKNLEGLMSIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWE--------------------------DISWLRSITSLPILIKGILTHE 300
           ANE LDASL WE                          DI WLRS TSLPILIKGILTHE
Sbjct: 241 ANETLDASLSWEVCPISCSDSFYFYFLSSLGIIFYHPQDIMWLRSTTSLPILIKGILTHE 300

Query: 301 DATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFK 360
           DATKAVE GVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFK
Sbjct: 301 DATKAVEGGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFK 360

Query: 361 ALALGAQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRT 404
           ALALGAQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRT
Sbjct: 361 ALALGAQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRT 420

[Close]

BLAST of CmoCh14G012300 vs. ExPASy TrEMBL
Match: A0A6J1GX02 ((S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.4e-203
Identity = 377/403 (93.55%), Postives = 377/403 (93.55%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
           AHKLAFHEGEQATARAAAAAKTIM                          IFKRRDISAL
Sbjct: 121 AHKLAFHEGEQATARAAAAAKTIM--------------------------IFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
           ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA
Sbjct: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 377

[Close]

BLAST of CmoCh14G012300 vs. ExPASy TrEMBL
Match: A0A6J1GUN9 ((S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.3e-200
Identity = 368/403 (91.32%), Postives = 384/403 (95.29%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIK KMSSEPVNVNDFKELAR  LPK
Sbjct: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKGKMSSEPVNVNDFKELARNALPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           +YYD+YAGGAEDEHTLRENIQAF RI I+PRVLVDVSQIDMSTTILG+ ISAPIL+APT+
Sbjct: 61  IYYDYYAGGAEDEHTLRENIQAFHRITIRPRVLVDVSQIDMSTTILGYPISAPILLAPTS 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
           AHKLA  EGE ATARAAAAA TIMILSYSSTCS+EEVASSCNAVRFFQL+I KRRDISAL
Sbjct: 121 AHKLAIPEGELATARAAAAANTIMILSYSSTCSMEEVASSCNAVRFFQLHISKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADI+NKMI  PVKNLEGL+SIDV SDQGSKLE Y
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIRNKMIGFPVKNLEGLMSIDVESDQGSKLEAY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
            NE+LDAS+RWEDI WLRSIT+LPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA
Sbjct: 241 YNELLDASIRWEDIGWLRSITNLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PAT+SVLEEVV AVKGK+ VLLDGGVRRGTDVFKA+ALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATVSVLEEVVDAVKGKVAVLLDGGVRRGTDVFKAVALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 403

[Close]

BLAST of CmoCh14G012300 vs. NCBI nr
Match: XP_022955687.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 779.6 bits (2012), Expect = 1.3e-221
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
           AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL
Sbjct: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
           ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA
Sbjct: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 403

[Close]

BLAST of CmoCh14G012300 vs. NCBI nr
Match: XP_023527007.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.3 bits (1980), Expect = 6.5e-218
Identity = 397/403 (98.51%), Postives = 399/403 (99.01%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           M+H FISKISVVN LNF T SPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MVHVFISKISVVNYLNFSTRSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQID+STTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDLSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
           AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL
Sbjct: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
           ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA
Sbjct: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 403

[Close]

BLAST of CmoCh14G012300 vs. NCBI nr
Match: XP_022980627.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 748.4 bits (1931), Expect = 3.1e-212
Identity = 388/403 (96.28%), Postives = 392/403 (97.27%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVN LNF THSPLKAAPSSLGQNYNIK KMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MLHAFISKISVVNYLNFSTHSPLKAAPSSLGQNYNIKGKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMI+PRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIRPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
            HKLAFHEGE ATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL
Sbjct: 121 GHKLAFHEGELATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPP+KNLEGL+SIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPMKNLEGLMSIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
           ANE LDASL WEDI WLRS TSLPILIKGILTHEDATKAVE GVDGIIVSNHGARQLDFA
Sbjct: 241 ANETLDASLSWEDIMWLRSTTSLPILIKGILTHEDATKAVEGGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRT +DKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTQHDKLPSML 403

[Close]

BLAST of CmoCh14G012300 vs. NCBI nr
Match: XP_023527000.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 737.3 bits (1902), Expect = 7.2e-209
Identity = 378/403 (93.80%), Postives = 388/403 (96.28%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           M+H FISKISV+N  NF THSP KAAPSSLGQNYNIK KMSSEPVNVNDFKE+ARLKLPK
Sbjct: 1   MVHVFISKISVLNYFNFSTHSPRKAAPSSLGQNYNIKGKMSSEPVNVNDFKEMARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMI+PRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIRPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
           AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYI+KRRDISAL
Sbjct: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIYKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           L QRAERFGYKAIVLTAD PRLGRREADIKNKMI PP+KNL+GL+S DVN+D+GSK ETY
Sbjct: 181 LAQRAERFGYKAIVLTADAPRLGRREADIKNKMIRPPMKNLDGLMSFDVNADEGSKFETY 240

Query: 241 ANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300
           AN  LDASLRWEDI WLRS TSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA
Sbjct: 241 ANMTLDASLRWEDIMWLRSTTSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFA 300

Query: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360
           PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG
Sbjct: 301 PATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGG 360

Query: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML
Sbjct: 361 VRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 403

[Close]

BLAST of CmoCh14G012300 vs. NCBI nr
Match: XP_022980626.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 734.2 bits (1894), Expect = 6.1e-208
Identity = 388/429 (90.44%), Postives = 392/429 (91.38%), Query Frame = 0


Query: 1   MLHAFISKISVVNNLNFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPK 60
           MLHAFISKISVVN LNF THSPLKAAPSSLGQNYNIK KMSSEPVNVNDFKELARLKLPK
Sbjct: 1   MLHAFISKISVVNYLNFSTHSPLKAAPSSLGQNYNIKGKMSSEPVNVNDFKELARLKLPK 60

Query: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120
           MYYDFYAGGAEDEHTLRENIQAFCRIMI+PRVLVDVSQIDMSTTILGHRISAPILVAPTA
Sbjct: 61  MYYDFYAGGAEDEHTLRENIQAFCRIMIRPRVLVDVSQIDMSTTILGHRISAPILVAPTA 120

Query: 121 AHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180
            HKLAFHEGE ATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL
Sbjct: 121 GHKLAFHEGELATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISAL 180

Query: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGLISIDVNSDQGSKLETY 240
           LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPP+KNLEGL+SIDVNSDQGSKLETY
Sbjct: 181 LVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPMKNLEGLMSIDVNSDQGSKLETY 240

Query: 241 ANEMLDASLRWE--------------------------DISWLRSITSLPILIKGILTHE 300
           ANE LDASL WE                          DI WLRS TSLPILIKGILTHE
Sbjct: 241 ANETLDASLSWEVCPISCSDSFYFYFLSSLGIIFYHPQDIMWLRSTTSLPILIKGILTHE 300

Query: 301 DATKAVEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFK 360
           DATKAVE GVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFK
Sbjct: 301 DATKAVEGGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFK 360

Query: 361 ALALGAQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRT 404
           ALALGAQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRT
Sbjct: 361 ALALGAQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRT 420

[Close]

BLAST of CmoCh14G012300 vs. TAIR 10
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 531.2 bits (1367), Expect = 7.3e-151
Identity = 261/359 (72.70%), Postives = 316/359 (88.02%), Query Frame = 0


Query: 45  VNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTT 104
           VNV++F+ELA+  LPKMYYDFY GGAED+HTL EN+QAF RIM +PRVLVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 105 ILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAV 164
           +LG+ ISAPI++APTA HKLA  +GE ATA+AAAA  TIMI+S+ STC+IEEVASSCNAV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 165 RFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGL 224
           RF Q+Y++KRRD++A +V+RAE+ G+KAIVLT D PRLGRREADIKNKMI+P +KN EGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 225 ISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGV 284
           +S +V  ++GS +E +A+   DASL W+DI WLRSIT LPIL+KG+LT EDA KAVEAGV
Sbjct: 185 VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGV 244

Query: 285 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 344
           DGI+VSNHGARQLD++PATI+VLEEVVHAVKG+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 345 IGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           IGRP+++GLAAKGE GV+ V++MLKNE EI+MALSGCP I D+TR+HVRT  +++ SML
Sbjct: 305 IGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTENERIKSML 363

[Close]

BLAST of CmoCh14G012300 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 9.9e-148
Identity = 259/359 (72.14%), Postives = 308/359 (85.79%), Query Frame = 0


Query: 45  VNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTT 104
           VNV++F+ELA+  LPKMYYDFY GGAED+HTL EN+QAF RIM +PRVLVDVS+IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 105 ILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAV 164
           ILG+ ISAPI++APT  HKLA  EGE ATA+AAAA  TIMI+SY S+C+ EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 165 RFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGL 224
           RF Q+Y++KRRDI+A +V+RAE+ G+KAIVLT D PRLGRREADIKNKMI+P +KN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 225 ISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGV 284
            S +V   +GS ++ +A+   DAS  W+DI WLRSIT LPIL+KGILT EDA KAVEAGV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 285 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 344
           DGIIVSNHG RQLD++PATI+VLEEVV  V+G+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 345 IGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           IGRP+++GLAAKGE GV+ V++MLKNE EI+MALSGCP I DITR+HVRT  ++L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTENERLHSML 363

[Close]

BLAST of CmoCh14G012300 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 9.9e-148
Identity = 259/359 (72.14%), Postives = 308/359 (85.79%), Query Frame = 0


Query: 45  VNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMSTT 104
           VNV++F+ELA+  LPKMYYDFY GGAED+HTL EN+QAF RIM +PRVLVDVS+IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 105 ILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAV 164
           ILG+ ISAPI++APT  HKLA  EGE ATA+AAAA  TIMI+SY S+C+ EE+ASSCNAV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 165 RFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPVKNLEGL 224
           RF Q+Y++KRRDI+A +V+RAE+ G+KAIVLT D PRLGRREADIKNKMI+P +KN EGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 225 ISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGV 284
            S +V   +GS ++ +A+   DAS  W+DI WLRSIT LPIL+KGILT EDA KAVEAGV
Sbjct: 185 FSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGV 244

Query: 285 DGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVL 344
           DGIIVSNHG RQLD++PATI+VLEEVV  V+G+IPVLLDGGVRRGTDVFKALALGAQAVL
Sbjct: 245 DGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVL 304

Query: 345 IGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 404
           IGRP+++GLAAKGE GV+ V++MLKNE EI+MALSGCP I DITR+HVRT  ++L SML
Sbjct: 305 IGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTENERLHSML 363

[Close]

BLAST of CmoCh14G012300 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 426.0 bits (1094), Expect = 3.3e-119
Identity = 215/358 (60.06%), Postives = 277/358 (77.37%), Query Frame = 0


Query: 43  EPVNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMS 102
           E  NV ++  +A+ KLPKM YD+YA GAED+ TL+EN  AF RI+ +PR+L+DVS+IDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 103 TTILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCN 162
           TT+LG +IS PI+VAPTA  K+A  +GE ATARAA+AA TIM LS  +T S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 163 AVRFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPP---VK 222
            +RFFQLY++K R++   LV+RAER G+KAI LT DTPRLGRRE+DIKN+   PP   +K
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 223 NLEGLISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKA 282
           N EGL    ++    S L +Y    +D +L W+D+ WL++IT LPIL+KG+LT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 283 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 342
           ++AG  GIIVSNHGARQLD+ PATIS LEEVV A +G+IPV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 343 AQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYD 398
           A  + IGRPV+F LAA+GE GVR VL+ML++E E++MALSGC  +K+I+R+H+ T +D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

[Close]

BLAST of CmoCh14G012300 vs. TAIR 10
Match: AT3G14420.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 426.0 bits (1094), Expect = 3.3e-119
Identity = 215/358 (60.06%), Postives = 277/358 (77.37%), Query Frame = 0


Query: 43  EPVNVNDFKELARLKLPKMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDMS 102
           E  NV ++  +A+ KLPKM YD+YA GAED+ TL+EN  AF RI+ +PR+L+DVS+IDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 103 TTILGHRISAPILVAPTAAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCN 162
           TT+LG +IS PI+VAPTA  K+A  +GE ATARAA+AA TIM LS  +T S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 163 AVRFFQLYIFKRRDISALLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPP---VK 222
            +RFFQLY++K R++   LV+RAER G+KAI LT DTPRLGRRE+DIKN+   PP   +K
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 223 NLEGLISIDVNSDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKA 282
           N EGL    ++    S L +Y    +D +L W+D+ WL++IT LPIL+KG+LT EDA  A
Sbjct: 182 NFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIA 241

Query: 283 VEAGVDGIIVSNHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALG 342
           ++AG  GIIVSNHGARQLD+ PATIS LEEVV A +G+IPV LDGGVRRGTDVFKALALG
Sbjct: 242 IQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALG 301

Query: 343 AQAVLIGRPVLFGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYD 398
           A  + IGRPV+F LAA+GE GVR VL+ML++E E++MALSGC  +K+I+R+H+ T +D
Sbjct: 302 ASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWD 359

The following BLAST results are available for this feature:

BLAST of CmoCh14G012300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJH5	1.0e-149	72.70	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q24JJ8	1.4e-146	72.14	Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8H3I4	3.0e-133	65.37	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=3...	[more]
B8B8K5	7.4e-132	65.10	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=399...	[more]
B8AKX6	4.2e-119	60.56	Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of CmoCh14G012300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GUB4	6.2e-222	100.00	(S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV...	[more]
A0A6J1IRU2	1.5e-212	96.28	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479940 PE=3 SV=1	[more]
A0A6J1IU35	3.0e-208	90.44	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479940 PE=3 SV=1	[more]
A0A6J1GX02	6.4e-203	93.55	(S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV...	[more]
A0A6J1GUN9	1.3e-200	91.32	(S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV...	[more]

BLAST of CmoCh14G012300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022955687.1	1.3e-221	100.00	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita moschata]	[more]
XP_023527007.1	6.5e-218	98.51	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita pepo subs...	[more]
XP_022980627.1	3.1e-212	96.28	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima]	[more]
XP_023527000.1	7.2e-209	93.80	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita pepo subs...	[more]
XP_022980626.1	6.1e-208	90.44	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima]	[more]

BLAST of CmoCh14G012300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14130.1	7.3e-151	72.70	Aldolase-type TIM barrel family protein	[more]
AT3G14150.1	9.9e-148	72.14	Aldolase-type TIM barrel family protein	[more]
AT3G14150.2	9.9e-148	72.14	Aldolase-type TIM barrel family protein	[more]
AT3G14420.1	3.3e-119	60.06	Aldolase-type TIM barrel family protein	[more]
AT3G14420.2	3.3e-119	60.06	Aldolase-type TIM barrel family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 42..402 e-value: 5.5E-158 score: 527.5
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	PIRSF	PIRSF000138	Al-hdrx_acd_dh	coord: 26..396 e-value: 8.3E-137 score: 454.1
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	CDD	cd02809	alpha_hydroxyacid_oxid_FMN	coord: 48..389 e-value: 6.09208E-157 score: 442.272
IPR000262	FMN-dependent dehydrogenase	PFAM	PF01070	FMN_dh	coord: 54..393 e-value: 3.1E-132 score: 440.8
None	No IPR available	PIRSR	PIRSR000138-2	PIRSR000138-2	coord: 39..394 e-value: 5.6E-138 score: 457.7
None	No IPR available	PANTHER	PTHR10578	S -2-HYDROXY-ACID OXIDASE-RELATED	coord: 40..393
None	No IPR available	PANTHER	PTHR10578:SF116	PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO4-LIKE	coord: 40..393
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 44..392
IPR008259	FMN-dependent alpha-hydroxy acid dehydrogenase, active site	PROSITE	PS00557	FMN_HYDROXY_ACID_DH_1	coord: 290..296
IPR037396	FMN hydroxy acid dehydrogenase domain	PROSITE	PS51349	FMN_HYDROXY_ACID_DH_2	coord: 39..397 score: 110.513824

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh14G012300.1	CmoCh14G012300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009854	oxidative photosynthetic carbon pathway
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0003973	(S)-2-hydroxy-acid oxidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity