CmoCh14G010720 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh14G010720
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationCmo_Chr14: 5903459 .. 5904766 (+)
RNA-Seq ExpressionCmoCh14G010720
SyntenyCmoCh14G010720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGTAAGGCGGCGCCGGCGAGGAATGTTCTGTTTGGAAAATACGAAATGGGTCGGCTATTGGGGAAAGGAACCTTTGCGAAGGTCTATCATGGAACAAACTTGATGACCCACGACAGTGTCGCCATTAAAGTGATCCTCAAGGACAGGGTAAAGAAAGAGGGACTTCTTGAGCAAGTCCGGCGAGAAATTTCTGTCATGGGGTTGGTTCGTCATCCCCATATAGTGGAATTGAAAGAAGTAATGGCCACGAAATCCAAGGTGTATTTTGTGATGGAATACGTTAATGGAGGCGAACTGTTTGTTAAATTGTCTCAACAAGGAAAATTGACAGAAGATGGAGGAAGAAAGTACTTTCAACAGCTTCTCTCCGCCGTGGACTTTTGCCACAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAAAATCTCCTTCTCGACGTGAATGGGAATTTGAAGGTATCTGATTTTGGCCTCTCCGCCCTGCCGGAGCAGCTCCGGATCGACGGATTGCTTCACACACGGTGTGGAACACCGGCGTATGTAGCGCCTGAGGTGCTCAGAAAGAGGGGATACGAGGGCGCTAAGTCGGATCTTTGGTCTTGTGGAGTGATTTTGTTTGTGTTGCTTGCTGGGTCTCTGCCATTTAAAGCTGATAATGTGATGAAGTTGTATAGGAAGATTTTCAAGGCGGAGTTTGAGTTTCCTCCTTGGTTTTCTTCAGAAGCTAAGGAAATGGTGTCAAAGCTTCTGGTTGTTGATCCTGAGAAGAGAGCTTCAATGGATGAGATAATGCAGAGTTGTTGGTTTCAAAAGGGCTTCACAAAGCCACTTTCATTCAATGACAAAGACGAACTCAACAGTGAAATCTCTGACTTGACAAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTCTACAACGCCTTCGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACTTGACTAGTTTGTTTGAAAGCAAGAAGAAACAAGCCTCAATTTTCACTTCAAAATGCTCTGCTTCAGCCATTATAGCCAAGCTTCAGTCTCTAGCGCAGAAGCTGAACTTCAAGGCTACTAATCAGAGGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGCTGGCGGTGACGGTGGAGGTTTACGAGGTAGCGCCGGAGCTGGCGGTGGTCGAGTTCTCGAAGTCGGCAGGGGACACATTGGAGTACAATAAGTTTTGTGAGAAGGATGTGAGGCCTGCATTGAAGGACATCGTTTGGAGCTGGCAAGGAGAGAATGAGTGCCTATAA

mRNA sequence

ATGGCGGGTAAGGCGGCGCCGGCGAGGAATGTTCTGTTTGGAAAATACGAAATGGGTCGGCTATTGGGGAAAGGAACCTTTGCGAAGGTCTATCATGGAACAAACTTGATGACCCACGACAGTGTCGCCATTAAAGTGATCCTCAAGGACAGGGTAAAGAAAGAGGGACTTCTTGAGCAAGTCCGGCGAGAAATTTCTGTCATGGGGTTGGTTCGTCATCCCCATATAGTGGAATTGAAAGAAGTAATGGCCACGAAATCCAAGGTGTATTTTGTGATGGAATACGTTAATGGAGGCGAACTGTTTGTTAAATTGTCTCAACAAGGAAAATTGACAGAAGATGGAGGAAGAAAGTACTTTCAACAGCTTCTCTCCGCCGTGGACTTTTGCCACAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAAAATCTCCTTCTCGACGTGAATGGGAATTTGAAGGTATCTGATTTTGGCCTCTCCGCCCTGCCGGAGCAGCTCCGGATCGACGGATTGCTTCACACACGGTGTGGAACACCGGCGTATGTAGCGCCTGAGGTGCTCAGAAAGAGGGGATACGAGGGCGCTAAGTCGGATCTTTGGTCTTGTGGAGTGATTTTGTTTGTGTTGCTTGCTGGGTCTCTGCCATTTAAAGCTGATAATGTGATGAAGTTGTATAGGAAGATTTTCAAGGCGGAGTTTGAGTTTCCTCCTTGGTTTTCTTCAGAAGCTAAGGAAATGGTGTCAAAGCTTCTGGTTGTTGATCCTGAGAAGAGAGCTTCAATGGATGAGATAATGCAGAGTTGTTGGTTTCAAAAGGGCTTCACAAAGCCACTTTCATTCAATGACAAAGACGAACTCAACAGTGAAATCTCTGACTTGACAAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTCTACAACGCCTTCGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACTTGACTAGTTTGTTTGAAAGCAAGAAGAAACAAGCCTCAATTTTCACTTCAAAATGCTCTGCTTCAGCCATTATAGCCAAGCTTCAGTCTCTAGCGCAGAAGCTGAACTTCAAGGCTACTAATCAGAGGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGCTGGCGGTGACGGTGGAGGTTTACGAGGTAGCGCCGGAGCTGGCGGTGGTCGAGTTCTCGAAGTCGGCAGGGGACACATTGGAGTACAATAAGTTTTGTGAGAAGGATGTGAGGCCTGCATTGAAGGACATCGTTTGGAGCTGGCAAGGAGAGAATGAGTGCCTATAA

Coding sequence (CDS)

ATGGCGGGTAAGGCGGCGCCGGCGAGGAATGTTCTGTTTGGAAAATACGAAATGGGTCGGCTATTGGGGAAAGGAACCTTTGCGAAGGTCTATCATGGAACAAACTTGATGACCCACGACAGTGTCGCCATTAAAGTGATCCTCAAGGACAGGGTAAAGAAAGAGGGACTTCTTGAGCAAGTCCGGCGAGAAATTTCTGTCATGGGGTTGGTTCGTCATCCCCATATAGTGGAATTGAAAGAAGTAATGGCCACGAAATCCAAGGTGTATTTTGTGATGGAATACGTTAATGGAGGCGAACTGTTTGTTAAATTGTCTCAACAAGGAAAATTGACAGAAGATGGAGGAAGAAAGTACTTTCAACAGCTTCTCTCCGCCGTGGACTTTTGCCACAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAAAATCTCCTTCTCGACGTGAATGGGAATTTGAAGGTATCTGATTTTGGCCTCTCCGCCCTGCCGGAGCAGCTCCGGATCGACGGATTGCTTCACACACGGTGTGGAACACCGGCGTATGTAGCGCCTGAGGTGCTCAGAAAGAGGGGATACGAGGGCGCTAAGTCGGATCTTTGGTCTTGTGGAGTGATTTTGTTTGTGTTGCTTGCTGGGTCTCTGCCATTTAAAGCTGATAATGTGATGAAGTTGTATAGGAAGATTTTCAAGGCGGAGTTTGAGTTTCCTCCTTGGTTTTCTTCAGAAGCTAAGGAAATGGTGTCAAAGCTTCTGGTTGTTGATCCTGAGAAGAGAGCTTCAATGGATGAGATAATGCAGAGTTGTTGGTTTCAAAAGGGCTTCACAAAGCCACTTTCATTCAATGACAAAGACGAACTCAACAGTGAAATCTCTGACTTGACAAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTCTACAACGCCTTCGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACTTGACTAGTTTGTTTGAAAGCAAGAAGAAACAAGCCTCAATTTTCACTTCAAAATGCTCTGCTTCAGCCATTATAGCCAAGCTTCAGTCTCTAGCGCAGAAGCTGAACTTCAAGGCTACTAATCAGAGGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGCTGGCGGTGACGGTGGAGGTTTACGAGGTAGCGCCGGAGCTGGCGGTGGTCGAGTTCTCGAAGTCGGCAGGGGACACATTGGAGTACAATAAGTTTTGTGAGAAGGATGTGAGGCCTGCATTGAAGGACATCGTTTGGAGCTGGCAAGGAGAGAATGAGTGCCTATAA

Protein sequence

MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQVRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLLSSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKATNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIVWSWQGENECL
Homology
BLAST of CmoCh14G010720 vs. ExPASy Swiss-Prot
Match: Q8W1D5 (CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CIPK25 PE=2 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 4.7e-164
Identity = 283/430 (65.81%), Postives = 351/430 (81.63%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQVRREISVMGL 70
           VLF KYEMGRLLGKGTF KVY+G  + T +SVAIK+I KD+VK+EG++EQ++REIS+M L
Sbjct: 38  VLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREISIMRL 97

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDFC 130
           VRHP+IVELKEVMATK+K++F+MEYV GGELF K+  +GKL ED  RKYFQQL+SAVDFC
Sbjct: 98  VRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKI-VKGKLKEDSARKYFQQLISAVDFC 157

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGVSHRDLKPENLL+D NG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPEVLRK
Sbjct: 158 HSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPEVLRK 217

Query: 191 RGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GY+GAK D+WSCG+IL+VLLAG LPF+ +N+MK+YRKIFK+EFE+PPWFS E+K ++SK
Sbjct: 218 KGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKRLISK 277

Query: 251 LLVVDPEKRASMDEIMQSCWFQKGFTKPLSF--------NDKDELNSEISDLTNPKLLSS 310
           LLVVDP KR S+  IM++ WF+K    P+ F        N +DE  +  +          
Sbjct: 278 LLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTTTTPV 337

Query: 311 SPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKATN 370
           SP F+NAFEFISSMSSGFDL+SLFESK+K  S+FTS+ SAS I+ KL+ + +++N K   
Sbjct: 338 SPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNMKVKR 397

Query: 371 QREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPALK 430
            ++FKV+L  K EGRKG++AVT EV+EVAPE+AVVE  KSAGDTLEYN+  E+ VRPAL+
Sbjct: 398 TKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVRPALE 457

Query: 431 DIVWSWQGEN 433
           +IVWSW G+N
Sbjct: 458 EIVWSWHGDN 466

BLAST of CmoCh14G010720 vs. ExPASy Swiss-Prot
Match: Q9LEU7 (CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CIPK5 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 6.7e-163
Identity = 278/438 (63.47%), Postives = 363/438 (82.88%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRV-KKEGLLEQVRREISV 68
           R VLFGKYEMGRLLGKGTFAKVY+G  ++  + VAIKVI KD+V K+ G++EQ++REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S +GKL ED  R+YFQQL+SAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKIS-KGKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLD NG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEM 248
           L+K+GY+GAK+D+WSCGV+L+VLLAG LPF+ +N+M +YRKIF+A+FEFPPWFS EA+ +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSF-------------NDKDELNSEISDL 308
           +SKLLVVDP++R S+  IM++ W +K FT PL+F             N+++E + +  + 
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 TNPKLLSSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQ 368
           T P     SP F+NAFEFISSMSSGFDL+SLFESK+K  S+FTS+ SA+ ++ K++++ +
Sbjct: 305 TEP----ISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTK 364

Query: 369 KLNFKATNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCE 428
           ++N K    ++FKV+++ K EGRKG+L++T EV+EVAPE++VVEF KSAGDTLEY++  E
Sbjct: 365 EMNMKVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYE 424

Query: 429 KDVRPALKDIVWSWQGEN 433
           ++VRPAL DIVWSW G+N
Sbjct: 425 EEVRPALNDIVWSWHGDN 437

BLAST of CmoCh14G010720 vs. ExPASy Swiss-Prot
Match: Q9SEZ7 (CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=3702 GN=CIPK16 PE=1 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 8.0e-148
Identity = 265/439 (60.36%), Postives = 339/439 (77.22%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRV-KKEGLLEQVRREISVMG 70
           VLF KY +GRLLG G FAKVYHGT + T D VAIKVI KD V K+ G++EQ+ REI+VM 
Sbjct: 10  VLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMR 69

Query: 71  LVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDF 130
           L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED  RKYFQQL+SAVDF
Sbjct: 70  LLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDF 129

Query: 131 CHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSAL--PEQL-----RIDGLLHTRCGTPAY 190
           CHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHTRCGTPAY
Sbjct: 130 CHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAY 189

Query: 191 VAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSS 250
           VAPEVLR +GY+GA +D+WSCG++L+ LLAG LPF  +NVM LY KIFKAE EFPPWFS 
Sbjct: 190 VAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSL 249

Query: 251 EAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNS--------EISDL 310
           E+KE++S+LLV DPE+R SM EI    WF+K FT  ++F+  + + S        +  DL
Sbjct: 250 ESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDL 309

Query: 311 TNPKLLSSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQ 370
              +   +SP  +NAF+FI+SMSSGFDL++LFE K+K   +FTSK  A ++  +L++ A+
Sbjct: 310 NEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAR 369

Query: 371 KLNFKATNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCE 430
           +++ +  + ++ K++LQ + EGRKG+L+VT EV+EVAPE++VVEF K++GDTLEY  FCE
Sbjct: 370 EMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCE 429

Query: 431 KDVRPALKDIVWSWQGENE 434
            DVRPALKDIVWSWQG+++
Sbjct: 430 DDVRPALKDIVWSWQGDDD 448

BLAST of CmoCh14G010720 vs. ExPASy Swiss-Prot
Match: Q6ERS4 (CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK16 PE=2 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-142
Identity = 247/426 (57.98%), Postives = 336/426 (78.87%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKK-EGLLEQVRREISV 68
           R ++ G+YE+GRLLG+GTFAKVY+G +L + +SVAIKVI K R+++ EG++EQ+RREIS+
Sbjct: 15  RKLVLGRYELGRLLGQGTFAKVYYGRDLRSGESVAIKVIDKARLRRTEGMVEQLRREISI 74

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAV 128
           M +VRHP++V ++EV+A++++V+ VMEY  GGELF K++ +G+LTE+  R+YFQQL++AV
Sbjct: 75  MRMVRHPNVVGIREVLASRARVFVVMEYARGGELFAKVA-RGRLTEEHARRYFQQLVAAV 134

Query: 129 DFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
            FCH RGV+HRDLKPENLLLD  G LKV+DFGL+ALPEQLR DGLLHT+CGTPAYVAPEV
Sbjct: 135 GFCHGRGVAHRDLKPENLLLDEEGRLKVTDFGLAALPEQLRQDGLLHTQCGTPAYVAPEV 194

Query: 189 LRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEM 248
           LRKRGY+GA++DLWSCGV+L+VLL G LPF+ +N  K+Y+KIFKAE++ PPW S +A+ +
Sbjct: 195 LRKRGYDGARADLWSCGVVLYVLLCGFLPFQHENYAKMYQKIFKAEYQVPPWVSGDARRL 254

Query: 249 VSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISD--LTNPKLLSSSPP 308
           + +LLVVDP KR S+ EIM++ WF+KGF  P+  +       E  D  L      + SP 
Sbjct: 255 IVRLLVVDPAKRISIPEIMRTPWFKKGFVPPVPTSPVSPKKWEEDDVLLDGGDSGAMSPR 314

Query: 309 FYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKATNQRE 368
             NAF+ ISSMSSGFDL+ +FES++K A++FTS+  A+ +I KL+++ + L + AT  + 
Sbjct: 315 TCNAFQLISSMSSGFDLSGMFESEQKAATVFTSRAPAATVIQKLEAVGRSLGYSATRGKG 374

Query: 369 FKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIV 428
           +K+RL+A  +G  G+LAVTVE  EVA ++AVVEF+  AGD LE+NKFC  DVRP L DIV
Sbjct: 375 WKLRLEATADGANGRLAVTVEALEVAADVAVVEFAHDAGDELEFNKFCAVDVRPGLADIV 434

Query: 429 WSWQGE 432
           W+WQG+
Sbjct: 435 WAWQGD 439

BLAST of CmoCh14G010720 vs. ExPASy Swiss-Prot
Match: Q9FJ54 (CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CIPK20 PE=1 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 3.2e-136
Identity = 242/425 (56.94%), Postives = 319/425 (75.06%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQVRREISVMGL 70
           VL  KYE+GRLLG+GTFAKVYH  N+ T +SVAIKVI K +V K GL++Q++REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S +GKL E+  RKYFQQL+ A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVS-KGKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLD NG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 RGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GY+GAK+D+WSCGV+L+VLLAG LPF   N++++YRKI K EF+ P WF  E K+++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPEKRASMDEIMQSCWFQKGFTK---PLSFNDKDELNSEISDLTNPKLLSSSPPFY 310
           +L  +P  R  +++IM++ WFQKGF K   P S  +  +++S ISD+      S  P  Y
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV--HAAFSVKPMSY 306

Query: 311 NAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKATNQREFK 370
           NAF+ ISS+S GFDL+ LFE +++  S FT+K  A  I++K + +A         + +  
Sbjct: 307 NAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVG 366

Query: 371 VRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIVWS 430
           V+++ K EGRKG LA+ VE++EV     +VEF KS GDT+EY +FC++++RP+LKDIVW 
Sbjct: 367 VKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWK 426

Query: 431 WQGEN 433
           WQG N
Sbjct: 427 WQGNN 427

BLAST of CmoCh14G010720 vs. ExPASy TrEMBL
Match: A0A6J1F2J4 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441609 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.0e-247
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60
           MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120
           VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300
           SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENECL 436
           LKDIVWSWQGENECL
Sbjct: 421 LKDIVWSWQGENECL 435

BLAST of CmoCh14G010720 vs. ExPASy TrEMBL
Match: A0A6J1J0B3 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111481539 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 7.1e-240
Identity = 424/435 (97.47%), Postives = 427/435 (98.16%), Query Frame = 0

Query: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60
           MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHG NLMTHDSVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120
           VRREISVMGLVRHPHIVELKEVMATKSKVYFVME VNGGELFVKLSQQGKLTEDGGRKYF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMECVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLLSAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300
           SSEAKEMVSKLLVVDPEKRASMDEIMQS WFQKGFTKPLSFNDKDE  SEIS+L NPK+L
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDEFKSEISELANPKML 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TNQREFKVRLQA EEGRKGKLAVT+EVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNQREFKVRLQAMEEGRKGKLAVTMEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENECL 436
           LKDIVWSWQGENECL
Sbjct: 421 LKDIVWSWQGENECL 435

BLAST of CmoCh14G010720 vs. ExPASy TrEMBL
Match: A0A5A7T8B4 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00290 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.2e-224
Identity = 394/433 (90.99%), Postives = 413/433 (95.38%), Query Frame = 0

Query: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60
           MAG+AA  RNVLFGKYEMGRLLGKGTFAKVY+G N+MTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDG R YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQ+GFTKP+SF +KDE N+E S+L NPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAIIAKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TN REFKVRLQAKEEGRKGKL VT EV+EVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 434
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of CmoCh14G010720 vs. ExPASy TrEMBL
Match: A0A1S3CN52 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103502821 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.2e-224
Identity = 394/433 (90.99%), Postives = 413/433 (95.38%), Query Frame = 0

Query: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60
           MAG+AA  RNVLFGKYEMGRLLGKGTFAKVY+G N+MTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDG R YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQ+GFTKP+SF +KDE N+E S+L NPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAIIAKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TN REFKVRLQAKEEGRKGKL VT EV+EVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 434
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of CmoCh14G010720 vs. ExPASy TrEMBL
Match: A0A6J1DJA1 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020996 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.1e-215
Identity = 375/435 (86.21%), Postives = 412/435 (94.71%), Query Frame = 0

Query: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60
           MAG+AAP+RNVLFGKYEMGRLLGKGTFAKVY+G NL+TH+SVAIKVILK+RVKKEGLL+Q
Sbjct: 1   MAGEAAPSRNVLFGKYEMGRLLGKGTFAKVYYGRNLVTHESVAIKVILKERVKKEGLLDQ 60

Query: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120
           ++REISVMGLVRHPHIVELKEVMATKSKVYFVMEYV+GGELFVKLS+QGKLTED  RKYF
Sbjct: 61  IQREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVSGGELFVKLSKQGKLTEDAARKYF 120

Query: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           +QL+SAVDFCHSRGVSHRDLKPENLL+D NGNLKVSDFGLSALPEQLR+DGLLHTRCGTP
Sbjct: 121 RQLVSAVDFCHSRGVSHRDLKPENLLVDENGNLKVSDFGLSALPEQLRVDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGY+GAK D+WSCGVIL+VLLAG+LPF+ADNVMKLYRKIFKA+FEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKVDIWSCGVILYVLLAGALPFRADNVMKLYRKIFKADFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300
           SSE+ EM+SK LVVDPE R SM EIMQS WF+ GF+KP+SF++KDE N+EIS+L NPKL 
Sbjct: 241 SSESMEMISKFLVVDPENRVSMPEIMQSPWFRNGFSKPISFSEKDEFNNEISELGNPKL- 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360
            SSPPFYNAFEFIS+MSSGFDL+SLFESKKK  SIFTSKCSASAI+AKLQSLAQK+NFKA
Sbjct: 301 -SSPPFYNAFEFISTMSSGFDLSSLFESKKKSGSIFTSKCSASAIVAKLQSLAQKVNFKA 360

Query: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TN +E KVRLQAKEEGRKGKL VT EV+EVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNLKECKVRLQAKEEGRKGKLTVTAEVFEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENECL 436
           LKDIVWSWQGE+E L
Sbjct: 421 LKDIVWSWQGESEGL 433

BLAST of CmoCh14G010720 vs. TAIR 10
Match: AT5G25110.1 (CBL-interacting protein kinase 25 )

HSP 1 Score: 578.9 bits (1491), Expect = 3.3e-165
Identity = 283/430 (65.81%), Postives = 351/430 (81.63%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQVRREISVMGL 70
           VLF KYEMGRLLGKGTF KVY+G  + T +SVAIK+I KD+VK+EG++EQ++REIS+M L
Sbjct: 38  VLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREISIMRL 97

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDFC 130
           VRHP+IVELKEVMATK+K++F+MEYV GGELF K+  +GKL ED  RKYFQQL+SAVDFC
Sbjct: 98  VRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKI-VKGKLKEDSARKYFQQLISAVDFC 157

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGVSHRDLKPENLL+D NG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPEVLRK
Sbjct: 158 HSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPEVLRK 217

Query: 191 RGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GY+GAK D+WSCG+IL+VLLAG LPF+ +N+MK+YRKIFK+EFE+PPWFS E+K ++SK
Sbjct: 218 KGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKRLISK 277

Query: 251 LLVVDPEKRASMDEIMQSCWFQKGFTKPLSF--------NDKDELNSEISDLTNPKLLSS 310
           LLVVDP KR S+  IM++ WF+K    P+ F        N +DE  +  +          
Sbjct: 278 LLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTTTTPV 337

Query: 311 SPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKATN 370
           SP F+NAFEFISSMSSGFDL+SLFESK+K  S+FTS+ SAS I+ KL+ + +++N K   
Sbjct: 338 SPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNMKVKR 397

Query: 371 QREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPALK 430
            ++FKV+L  K EGRKG++AVT EV+EVAPE+AVVE  KSAGDTLEYN+  E+ VRPAL+
Sbjct: 398 TKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVRPALE 457

Query: 431 DIVWSWQGEN 433
           +IVWSW G+N
Sbjct: 458 EIVWSWHGDN 466

BLAST of CmoCh14G010720 vs. TAIR 10
Match: AT5G10930.1 (CBL-interacting protein kinase 5 )

HSP 1 Score: 575.1 bits (1481), Expect = 4.8e-164
Identity = 278/438 (63.47%), Postives = 363/438 (82.88%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRV-KKEGLLEQVRREISV 68
           R VLFGKYEMGRLLGKGTFAKVY+G  ++  + VAIKVI KD+V K+ G++EQ++REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S +GKL ED  R+YFQQL+SAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKIS-KGKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLD NG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEM 248
           L+K+GY+GAK+D+WSCGV+L+VLLAG LPF+ +N+M +YRKIF+A+FEFPPWFS EA+ +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSF-------------NDKDELNSEISDL 308
           +SKLLVVDP++R S+  IM++ W +K FT PL+F             N+++E + +  + 
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 TNPKLLSSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQ 368
           T P     SP F+NAFEFISSMSSGFDL+SLFESK+K  S+FTS+ SA+ ++ K++++ +
Sbjct: 305 TEP----ISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTK 364

Query: 369 KLNFKATNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCE 428
           ++N K    ++FKV+++ K EGRKG+L++T EV+EVAPE++VVEF KSAGDTLEY++  E
Sbjct: 365 EMNMKVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYE 424

Query: 429 KDVRPALKDIVWSWQGEN 433
           ++VRPAL DIVWSW G+N
Sbjct: 425 EEVRPALNDIVWSWHGDN 437

BLAST of CmoCh14G010720 vs. TAIR 10
Match: AT2G25090.1 (CBL-interacting protein kinase 16 )

HSP 1 Score: 525.0 bits (1351), Expect = 5.7e-149
Identity = 265/439 (60.36%), Postives = 339/439 (77.22%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRV-KKEGLLEQVRREISVMG 70
           VLF KY +GRLLG G FAKVYHGT + T D VAIKVI KD V K+ G++EQ+ REI+VM 
Sbjct: 10  VLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMR 69

Query: 71  LVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDF 130
           L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED  RKYFQQL+SAVDF
Sbjct: 70  LLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDF 129

Query: 131 CHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSAL--PEQL-----RIDGLLHTRCGTPAY 190
           CHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHTRCGTPAY
Sbjct: 130 CHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAY 189

Query: 191 VAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSS 250
           VAPEVLR +GY+GA +D+WSCG++L+ LLAG LPF  +NVM LY KIFKAE EFPPWFS 
Sbjct: 190 VAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSL 249

Query: 251 EAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNS--------EISDL 310
           E+KE++S+LLV DPE+R SM EI    WF+K FT  ++F+  + + S        +  DL
Sbjct: 250 ESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDL 309

Query: 311 TNPKLLSSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQ 370
              +   +SP  +NAF+FI+SMSSGFDL++LFE K+K   +FTSK  A ++  +L++ A+
Sbjct: 310 NEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAR 369

Query: 371 KLNFKATNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCE 430
           +++ +  + ++ K++LQ + EGRKG+L+VT EV+EVAPE++VVEF K++GDTLEY  FCE
Sbjct: 370 EMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCE 429

Query: 431 KDVRPALKDIVWSWQGENE 434
            DVRPALKDIVWSWQG+++
Sbjct: 430 DDVRPALKDIVWSWQGDDD 448

BLAST of CmoCh14G010720 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 486.5 bits (1251), Expect = 2.2e-137
Identity = 242/425 (56.94%), Postives = 319/425 (75.06%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQVRREISVMGL 70
           VL  KYE+GRLLG+GTFAKVYH  N+ T +SVAIKVI K +V K GL++Q++REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S +GKL E+  RKYFQQL+ A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVS-KGKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLD NG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 RGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GY+GAK+D+WSCGV+L+VLLAG LPF   N++++YRKI K EF+ P WF  E K+++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPEKRASMDEIMQSCWFQKGFTK---PLSFNDKDELNSEISDLTNPKLLSSSPPFY 310
           +L  +P  R  +++IM++ WFQKGF K   P S  +  +++S ISD+      S  P  Y
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV--HAAFSVKPMSY 306

Query: 311 NAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKATNQREFK 370
           NAF+ ISS+S GFDL+ LFE +++  S FT+K  A  I++K + +A         + +  
Sbjct: 307 NAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVG 366

Query: 371 VRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIVWS 430
           V+++ K EGRKG LA+ VE++EV     +VEF KS GDT+EY +FC++++RP+LKDIVW 
Sbjct: 367 VKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWK 426

Query: 431 WQGEN 433
           WQG N
Sbjct: 427 WQGNN 427

BLAST of CmoCh14G010720 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 449.1 bits (1154), Expect = 4.0e-126
Identity = 234/423 (55.32%), Postives = 306/423 (72.34%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQVRREISVMGL 70
           +L G+YE+GRLLG GTFAKVYH  N+ T  SVA+KV+ K++V K G+++Q++REISVM +
Sbjct: 19  LLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRM 78

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYFQQLLSAVDFC 130
           V+HP+IVEL EVMA+KSK+YF ME V GGELF K++ +G+L ED  R YFQQL+SAVDFC
Sbjct: 79  VKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVA-KGRLREDVARVYFQQLISAVDFC 138

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLD  GNLKV+DFGLSA  E L+ DGLLHT CGTPAYVAPEV+ K
Sbjct: 139 HSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILK 198

Query: 191 RGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GY+GAK+DLWSCGVILFVLLAG LPF+ DN++ +YRKI++ +F+ P W SS+A+ +V+K
Sbjct: 199 KGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTK 258

Query: 251 LLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLLSSS---PPFY 310
           LL  +P  R +++++M S WF+K  T+  S N+        ++     L+  S       
Sbjct: 259 LLDPNPNTRITIEKVMDSPWFKKQATR--SRNEPVAATITTTEEDVDFLVHKSKEETETL 318

Query: 311 NAFEFISSMSSGFDLTSLFESKKKQAS---IFTSKCSASAIIAKLQSLAQKLNFKATNQR 370
           NAF  I ++S GFDL+ LFE KKK+      F +   AS++I+ L+  A+  N     + 
Sbjct: 319 NAFHII-ALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVRKS 378

Query: 371 EFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDI 428
           E +VR++ K+ GRKGKLAV  E++ VAP   VVE  K  GDTLEYN FC   +RPALKDI
Sbjct: 379 ESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALKDI 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W1D54.7e-16465.81CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=37... [more]
Q9LEU76.7e-16363.47CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=370... [more]
Q9SEZ78.0e-14860.36CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=37... [more]
Q6ERS41.7e-14257.98CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q9FJ543.2e-13656.94CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A6J1F2J46.0e-247100.00Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1J0B37.1e-24097.47Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5A7T8B43.2e-22490.99Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CN523.2e-22490.99Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1DJA12.1e-21586.21Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G25110.13.3e-16565.81CBL-interacting protein kinase 25 [more]
AT5G10930.14.8e-16463.47CBL-interacting protein kinase 5 [more]
AT2G25090.15.7e-14960.36CBL-interacting protein kinase 16 [more]
AT5G45820.12.2e-13756.94CBL-interacting protein kinase 20 [more]
AT4G30960.14.0e-12655.32SOS3-interacting protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 16..271
e-value: 6.1E-99
score: 344.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 16..271
e-value: 8.7E-75
score: 251.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 16..271
score: 50.604679
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 8..310
e-value: 7.2E-101
score: 339.6
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 311..427
e-value: 1.0E-37
score: 130.8
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 7..222
e-value: 1.8E-25
score: 87.4
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 10..274
e-value: 5.1E-44
score: 148.3
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 15..271
e-value: 1.0E-35
score: 120.4
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 9..434
NoneNo IPR availablePANTHERPTHR43895:SF119CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 25-RELATEDcoord: 9..434
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 308..422
e-value: 8.87442E-44
score: 147.334
IPR004041NAF domainPFAMPF03822NAFcoord: 304..360
e-value: 2.0E-16
score: 59.9
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 135..147
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 22..54
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 302..327
score: 12.177206
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 12..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh14G010720.1CmoCh14G010720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity