CmoCh14G009710 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh14G009710
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr14: 5186377 .. 5188787 (+)
RNA-Seq ExpressionCmoCh14G009710
SyntenyCmoCh14G009710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAATCAAGATCAAGAACAACAACATTAATGTTTGATCCAAGGCATAATCCATTTTGGGTTTGTGTAATTTCTCTTGCCATATTCATTCCATCAACAAGCTGCACCCCTACACCGCCAACTTTGATCAACTTCGGCGACTCGAACTCCGATACCGGCGGTGTTCTTGCTGGGACCGGACTCCCCATTGGCCTCCCCCACGGCATTACCTTCTTCCATCGTGGTACCGGGCGCTTCGGTGACGGTCGCCTCATAATTGACTTCTTCTGTGGGTTCCATACTATTTAGTAGGGTTTGTTTCAGATTTAGGCAGTCAAATGTTTGATGTTTGATGTTTTGGTGTTCAGGTGAAGACTTGAAACTGAGGTATTTGAGTCCATATTTGGAGTCCTTAGCTCCAAATTTTACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCGACTACACTTCCACAGTTTGTCCCTTTTGCGCTTGATGTTCAAATTCGCCAATTCATTCACTTCAAAAACCGTTCTCTTCACCTTCAATCATTAGGTATATGTTTTTCCTTTCTATTTTAATTTCTCCCTTTTTTGTGATCCATTTATGTCTAGTTTGATAACTATGAAATACCACATTGGTTAGAGAGAAGAATGAAATATTGTCTATGAGGGTGTGGAAATCTCTTCATAGCATACGCATTTTAAAAAGCTTGAGGAAAATCCCAAAAGGGAAAGCCAAAATAGAACAACATTTGCTAGCGGTAGACTTGGGTTATTACAAATGATATCATAGACAGACACTAGGGCGGTGTGCCAACAAGAATGCTGAGCCCCAAAGGGGGGTGGATTATGAGATTCCACATTGATTAGAGAGGAGAACGAGTGCTAGCGAAGACGCTGGGCCCTAAAGGAGAGTGGATTGTGGATCCCACGTCGATTCGAGAGGGGAATGGAACATTCTTTGTAAGGGTGTGGATTCGCTGGCCCCAAAGGAGGGTGGATTGTGAATCCCACGTCGATTGGAGAGGGGGAATGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTTAACAAACGCATTTTAAAAACCTTGAGCGAAACCATGGAAGGGAAAGCCCAAAGAGGACAGGATGCTGAGCCTCGAAGGGGGTGGATTGAGAGATCCCACATCAATTTTTTTTATAAGGGTGTGGGAAACCTCTCCCTAACAAACATGTTTTTAAAAATCGTGACAGAAAATCCAAAAATGAAAACTCAAAGAGAACAATATCTGTTAGTAGTGGGCTTAGACTGTTACAATAACAATTTCGACTCCCTACTTCTACTATCTATAAATTTCTTTGTTTCGTTATCAAGTATCTAATGTAGACGGGTTTGTGGCAGGTAAAACTAAGAACATAATGGATGAAGAAGGGTTTAAGAATGGAGTATACATGATTGACATTGGTCAGAATGACCTCTTAGTTGCCCTTTATGCATCAAATTTAACCTACAAACCAGTGGCCCAAAAGATCCCATCTTTTCTTGCAGAAATCAAGCTAGCCATTCAGGTGAACCCTTCTTCCACTTTTCCCTTCCAAATTCACCACTCTTAAAACCATTAACAATACACCAACTTGCACTCTTTTTTAATTATACACCACTTTATCACAAGGAATCTTTTGGATCATGTGCAAGCATTTTTAATATTTGAAAACACAACTTACAACATTGCGTACAGAATCTATATGCAAATGGTGGAAGAAAATTTTGGATACACAATACAGGACCATTAGGTTGCTCTCCAAAAGAGCTGGCATTGCATCCCCACACCCACAAGGATGTTGACCAAATTGGGTGCCTTAGGGTTCACAATCAAGTCGCCAAATCCTTCAATAAAGACCTTAAAAATGTGTGCAAAGACCTACGATCTCAATTTAAGGATGCCATCGTCGTCTACGTCGACGTATACACCATCAAATATAATCTCTTCGCCCATCCCGACGTATACGGTATGAAATCTCGAACAAAATTGTGAATAAATTTAAGGACTTTTTTGAGAGAATGTTTTGGTTTTTGTGAAAGGGTTTGAGAAGGCATTAATGGCATGTTGTGGGTATGGAGGGGCGCCAAATAATTACAACGTGAAGGCAACATGTGGGCAACCGGGGTACTCCATCTGCGCCAACCCTTCGAAAGCTATCGTTTGGGATGGAGTTCATTACAGTGAAGCTGCCAATCGTGTTGTGGCTTCCGCTATCTTATCTGCCCATTTCTCTACTCCTCAACTTACACTTCGCCAACTTTTCCTCCTTTAGCTTATCTTCTTCTTCTTCCATCTAAATTTCAAGTCTTTTCATCTCAATAATAACGTCGTGTTTATTATTTTCAAGTTTAGTCGATGAAAAATTTGTGCTTAACGTTGAATAACAAGTTTATCTCCCGAA

mRNA sequence

ATGACGAAATCAAGATCAAGAACAACAACATTAATGTTTGATCCAAGGCATAATCCATTTTGGGTTTGTGTAATTTCTCTTGCCATATTCATTCCATCAACAAGCTGCACCCCTACACCGCCAACTTTGATCAACTTCGGCGACTCGAACTCCGATACCGGCGGTGTTCTTGCTGGGACCGGACTCCCCATTGGCCTCCCCCACGGCATTACCTTCTTCCATCGTGGTACCGGGCGCTTCGGTGACGGTCGCCTCATAATTGACTTCTTCTGTGAAGACTTGAAACTGAGGTATTTGAGTCCATATTTGGAGTCCTTAGCTCCAAATTTTACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCGACTACACTTCCACAGTTTGTCCCTTTTGCGCTTGATGTTCAAATTCGCCAATTCATTCACTTCAAAAACCGTTCTCTTCACCTTCAATCATTAGGTAAAACTAAGAACATAATGGATGAAGAAGGGTTTAAGAATGGAGTATACATGATTGACATTGGTCAGAATGACCTCTTAGTTGCCCTTTATGCATCAAATTTAACCTACAAACCAGTGGCCCAAAAGATCCCATCTTTTCTTGCAGAAATCAAGCTAGCCATTCAGAATCTATATGCAAATGGTGGAAGAAAATTTTGGATACACAATACAGGACCATTAGGTTGCTCTCCAAAAGAGCTGGCATTGCATCCCCACACCCACAAGGATGTTGACCAAATTGGGTGCCTTAGGGTTCACAATCAAGTCGCCAAATCCTTCAATAAAGACCTTAAAAATGTGTGCAAAGACCTACGATCTCAATTTAAGGATGCCATCGTCGTCTACGTCGACGTATACACCATCAAATATAATCTCTTCGCCCATCCCGACGTATACGGGTTTGAGAAGGCATTAATGGCATGTTGTGGGTATGGAGGGGCGCCAAATAATTACAACGTGAAGGCAACATGTGGGCAACCGGGGTACTCCATCTGCGCCAACCCTTCGAAAGCTATCGTTTGGGATGGAGTTCATTACAGTGAAGCTGCCAATCGTGTTGTGGCTTCCGCTATCTTATCTGCCCATTTCTCTACTCCTCAACTTACACTTCGCCAACTTTTCCTCCTTTAGCTTATCTTCTTCTTCTTCCATCTAAATTTCAAGTCTTTTCATCTCAATAATAACGTCGTGTTTATTATTTTCAAGTTTAGTCGATGAAAAATTTGTGCTTAACGTTGAATAACAAGTTTATCTCCCGAA

Coding sequence (CDS)

ATGACGAAATCAAGATCAAGAACAACAACATTAATGTTTGATCCAAGGCATAATCCATTTTGGGTTTGTGTAATTTCTCTTGCCATATTCATTCCATCAACAAGCTGCACCCCTACACCGCCAACTTTGATCAACTTCGGCGACTCGAACTCCGATACCGGCGGTGTTCTTGCTGGGACCGGACTCCCCATTGGCCTCCCCCACGGCATTACCTTCTTCCATCGTGGTACCGGGCGCTTCGGTGACGGTCGCCTCATAATTGACTTCTTCTGTGAAGACTTGAAACTGAGGTATTTGAGTCCATATTTGGAGTCCTTAGCTCCAAATTTTACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCGACTACACTTCCACAGTTTGTCCCTTTTGCGCTTGATGTTCAAATTCGCCAATTCATTCACTTCAAAAACCGTTCTCTTCACCTTCAATCATTAGGTAAAACTAAGAACATAATGGATGAAGAAGGGTTTAAGAATGGAGTATACATGATTGACATTGGTCAGAATGACCTCTTAGTTGCCCTTTATGCATCAAATTTAACCTACAAACCAGTGGCCCAAAAGATCCCATCTTTTCTTGCAGAAATCAAGCTAGCCATTCAGAATCTATATGCAAATGGTGGAAGAAAATTTTGGATACACAATACAGGACCATTAGGTTGCTCTCCAAAAGAGCTGGCATTGCATCCCCACACCCACAAGGATGTTGACCAAATTGGGTGCCTTAGGGTTCACAATCAAGTCGCCAAATCCTTCAATAAAGACCTTAAAAATGTGTGCAAAGACCTACGATCTCAATTTAAGGATGCCATCGTCGTCTACGTCGACGTATACACCATCAAATATAATCTCTTCGCCCATCCCGACGTATACGGGTTTGAGAAGGCATTAATGGCATGTTGTGGGTATGGAGGGGCGCCAAATAATTACAACGTGAAGGCAACATGTGGGCAACCGGGGTACTCCATCTGCGCCAACCCTTCGAAAGCTATCGTTTGGGATGGAGTTCATTACAGTGAAGCTGCCAATCGTGTTGTGGCTTCCGCTATCTTATCTGCCCATTTCTCTACTCCTCAACTTACACTTCGCCAACTTTTCCTCCTTTAG

Protein sequence

MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSAHFSTPQLTLRQLFLL
Homology
BLAST of CmoCh14G009710 vs. ExPASy Swiss-Prot
Match: Q6NLP7 (GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.3e-135
Identity = 234/350 (66.86%), Postives = 279/350 (79.71%), Query Frame = 0

Query: 24  VISLAIFIPSTSCTP----TPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGR 83
           V+ L++ + S S T       P LINFGDSNSDTGGVLAG GLPIGLPHGITFFHRGTGR
Sbjct: 14  VLCLSLLVCSNSETSYKSNKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGR 73

Query: 84  FGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQF 143
            GDGRLI+DF+CE LK+ YLSPYL+SL+PNF  GVNFAVSGAT LP F  F L +QIRQF
Sbjct: 74  LGDGRLIVDFYCEHLKMTYLSPYLDSLSPNFKRGVNFAVSGATALPIF-SFPLAIQIRQF 133

Query: 144 IHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSF 203
           +HFKNRS  L S G+ ++++D+ GF+N +YMIDIGQNDLL+ALY SNLTY PV +KIPS 
Sbjct: 134 VHFKNRSQELISSGR-RDLIDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSM 193

Query: 204 LAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSF 263
           L EIK AIQ +Y  GGRKFW+HNTGPLGC+PKELA+H H   D+D IGC RVHN+VAK+F
Sbjct: 194 LLEIKKAIQTVYLYGGRKFWVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAF 253

Query: 264 NKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNV 323
           NK L ++C +LRSQFKDA +VYVD+Y+IKY L A   +YGF   LMACCGYGG PNNY+ 
Sbjct: 254 NKGLLSLCNELRSQFKDATLVYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDR 313

Query: 324 KATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSAHFSTPQLTL 370
           KATCGQPG +IC + +KAIVWDGVHY+EAANR V  A+L+  +S P+ +L
Sbjct: 314 KATCGQPGSTICRDVTKAIVWDGVHYTEAANRFVVDAVLTNRYSYPKNSL 361

BLAST of CmoCh14G009710 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 7.5e-102
Identity = 180/365 (49.32%), Postives = 247/365 (67.67%), Query Frame = 0

Query: 9   TTLMFDPRHNPFWVCVISLAIFI---PS--TSCTPTPPTLINFGDSNSDTGGVLAGTGLP 68
           T  ++    + F++ ++SLA+ I   PS   SCT   P + NFGDSNSDTGG++AG G P
Sbjct: 3   TLFLYSNTFSFFFITLVSLALLILRQPSRAASCT-ARPVIFNFGDSNSDTGGLVAGLGYP 62

Query: 69  IGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGAT 128
           IG P+G  FF R TGR  DGRL+IDF C+ L    L PYL+SL    F +G NFA++G+ 
Sbjct: 63  IGFPNGRLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSP 122

Query: 129 TLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKN--IMDEEGFKNGVYMIDIGQNDLLV 188
           TLP+ VPF+L++Q++QF HFK+RSL L S   +     +   GFKN +YMIDIGQND + 
Sbjct: 123 TLPKNVPFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQND-IA 182

Query: 189 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 248
             +A   +Y    + IP  + EIK +I+ LY  GGR+FWIHNTGPLGC P++L++     
Sbjct: 183 RSFARGNSYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKS 242

Query: 249 KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF 308
           KD+DQ GCL  +N  A  FN+ L ++C++LR++ +DA ++Y+D+Y IKY+L A+ + YGF
Sbjct: 243 KDLDQHGCLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGF 302

Query: 309 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSA 366
           +  LMACCGYGG P NYNVK TCG  G ++C   S+ I WDG+HY+E AN +VA  +LS 
Sbjct: 303 KSPLMACCGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVAMKVLSM 362

BLAST of CmoCh14G009710 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 9.8e-102
Identity = 176/329 (53.50%), Postives = 238/329 (72.34%), Query Frame = 0

Query: 40  PPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYL 99
           PP + NFGDSNSDTGG++AG G  IGLP+G +FF R TGR  DGRL+IDF C+ L    L
Sbjct: 35  PPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLSDGRLVIDFLCQSLNTSLL 94

Query: 100 SPYLESL-APNFTSGVNFAVSGATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGK--TK 159
           +PYL+SL    F +G NFA+ G++TLP++VPFAL++Q+ QF+HFK+R+L L S+     +
Sbjct: 95  NPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQLMQFLHFKSRALELASISDPLKE 154

Query: 160 NIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGR 219
            ++ E GF+N +YMIDIGQND+  + ++  L+Y  V + IP+ ++EIK AI+ LY  GGR
Sbjct: 155 MMIGESGFRNALYMIDIGQNDIADS-FSKGLSYSRVVKLIPNVISEIKSAIKILYDEGGR 214

Query: 220 KFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKD 279
           KFW+HNTGPLGC P++L++  H+ K  D+ GCL  +N  AK FN+ L ++C+DLR++ K+
Sbjct: 215 KFWVHNTGPLGCLPQKLSM-VHS-KGFDKHGCLATYNAAAKLFNEGLDHMCRDLRTELKE 274

Query: 280 AIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSK 339
           A +VYVD+Y IKY+L A+ + YGFEK LMACCGYGG P NYNV  TCG  G   C   S+
Sbjct: 275 ANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEGSR 334

Query: 340 AIVWDGVHYSEAANRVVASAILSAHFSTP 366
            I WDG+HY+E AN +VA  +LS   STP
Sbjct: 335 FISWDGIHYTETANAIVAMKVLSMQHSTP 360

BLAST of CmoCh14G009710 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.6e-75
Identity = 165/400 (41.25%), Postives = 225/400 (56.25%), Query Frame = 0

Query: 20  FWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGR 79
           F+V +  ++    S S     P+  NFGDSNSDTG ++AG G+ + LP+G   F   + R
Sbjct: 9   FYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 68

Query: 80  FGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPFALDV 139
           F DGRL+IDF  +++ L +L+PYL+SL  PNF  G NFA +G+T LP       PF+ D+
Sbjct: 69  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 128

Query: 140 QIRQFIHFKNRSLHLQSLGKT-----KNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 199
           QI QFI FK+R++ L  L KT     K +   + +  G+YMIDIGQND+  A Y+   T 
Sbjct: 129 QISQFIRFKSRAIEL--LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK--TL 188

Query: 200 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 259
             V   IPS L   +  ++ LY  GGR  WIHNTGPLGC  + +A        +D+ GC+
Sbjct: 189 DQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCV 248

Query: 260 RVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAH--------------- 319
             HNQ AK FN  L  +    ++Q+ DA V YVD+++IK NL A+               
Sbjct: 249 SSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDL 308

Query: 320 -----------PDVYGFEKALMACCGYGGAPNNYNVKATCGQ----PGYSI----CANPS 376
                       +V GFEK LMACCG GGAP NY+ + TCGQ     G S+    C + S
Sbjct: 309 NHLENVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSS 368

BLAST of CmoCh14G009710 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.1e-72
Identity = 147/336 (43.75%), Postives = 200/336 (59.52%), Query Frame = 0

Query: 41  PTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLS 100
           P + NFGDSNSDTGG+ A  G   G PHG +FF    GR+ DGRL+IDF  E L L YLS
Sbjct: 29  PAIFNFGDSNSDTGGLSAAFG-QAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPYLS 88

Query: 101 PYLESLAPNFTSGVNFAVSGA------TTLPQ--FVPFALDVQIRQFIHFKNRSLHLQSL 160
            +L+S+  NF+ G NFA +G+      +TL Q  F PF+LDVQ  QF +F NRS  ++S 
Sbjct: 89  AFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVRSR 148

Query: 161 GKTKNIM--DEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNL 220
           G     M  + + F   +Y  DIGQND L A Y +N T + V  ++P  +++   AI+N+
Sbjct: 149 GGVYKTMLPESDSFSKALYTFDIGQND-LTAGYFANKTVEQVETEVPEIISQFMNAIKNI 208

Query: 221 YANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKDL 280
           Y  GGR FWIHNTGP+GC    +   P+   D D  GC+   N +A+ FN  LK    +L
Sbjct: 209 YGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHALKQAVIEL 268

Query: 281 RSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNVKATCGQ----P 340
           RS   +A + YVDVY++K+ LF H   +GF+ +L++CCG+GG   NYN    CG      
Sbjct: 269 RSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGG-KYNYNKGIGCGMKKIVK 328

Query: 341 GYSI-----CANPSKAIVWDGVHYSEAANRVVASAI 358
           G  +     C  P KA+VWDGVH+++AAN+ +   I
Sbjct: 329 GKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKI 361

BLAST of CmoCh14G009710 vs. ExPASy TrEMBL
Match: A0A6J1FA19 (GDSL esterase/lipase At3g62280-like OS=Cucurbita moschata OX=3662 GN=LOC111442195 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 6.3e-221
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60
           MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT
Sbjct: 1   MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60

Query: 61  GLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120
           GLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG
Sbjct: 61  GLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120

Query: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180
           ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV
Sbjct: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180

Query: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240
           ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH
Sbjct: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240

Query: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF 300
           KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF
Sbjct: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF 300

Query: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSA 360
           EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSA
Sbjct: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSA 360

Query: 361 HFSTPQLTLRQLFLL 376
           HFSTPQLTLRQLFLL
Sbjct: 361 HFSTPQLTLRQLFLL 375

BLAST of CmoCh14G009710 vs. ExPASy TrEMBL
Match: A0A6J1J5S7 (GDSL esterase/lipase At3g62280-like OS=Cucurbita maxima OX=3661 GN=LOC111482013 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 1.9e-217
Identity = 368/375 (98.13%), Postives = 371/375 (98.93%), Query Frame = 0

Query: 1   MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60
           MTKSRSRTTTLMFDP+HNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT
Sbjct: 12  MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 71

Query: 61  GLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120
           GLPIGLPHGITFFH GTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG
Sbjct: 72  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 131

Query: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180
           ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV
Sbjct: 132 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 191

Query: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240
           ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH
Sbjct: 192 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 251

Query: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF 300
           KDVDQIGCLRVHNQVAKSFNKDLKNVCK LRSQFKDAIVVYVDVYTIKYNLF HPDVYGF
Sbjct: 252 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 311

Query: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSA 360
           EKALMACCGYGGAPNNYNVKATCGQPGYSICANP ++IVWDGVHYSEAANRVVASAILSA
Sbjct: 312 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIVWDGVHYSEAANRVVASAILSA 371

Query: 361 HFSTPQLTLRQLFLL 376
           HFSTPQLTLRQLFLL
Sbjct: 372 HFSTPQLTLRQLFLL 386

BLAST of CmoCh14G009710 vs. ExPASy TrEMBL
Match: A0A6J1DWR3 (GDSL esterase/lipase At3g62280 OS=Momordica charantia OX=3673 GN=LOC111023851 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 9.0e-183
Identity = 317/377 (84.08%), Postives = 341/377 (90.45%), Query Frame = 0

Query: 1   MTKSRSRTTTLMFDPRH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAG 60
           MT SRSR ++  FDP+  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAG
Sbjct: 7   MTISRSRNSS--FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAG 66

Query: 61  TGLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVS 120
           TGLPI LPHGITFFHRGTGR GDGRLIIDFFCE+LKL YLSPYLESLAPNFTSGVNFAVS
Sbjct: 67  TGLPISLPHGITFFHRGTGRLGDGRLIIDFFCEELKLTYLSPYLESLAPNFTSGVNFAVS 126

Query: 121 GATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLG-KTKN-IMDEEGFKNGVYMIDIGQND 180
           GATTLPQFVPFALD+Q+RQFIHFKNRSL LQSLG K +N +MDEEGF+NG YMIDIGQND
Sbjct: 127 GATTLPQFVPFALDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQND 186

Query: 181 LLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP 240
           LLVALYASNLTYKPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHP
Sbjct: 187 LLVALYASNLTYKPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHP 246

Query: 241 HTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDV 300
           H  +DVDQIGCLRVHNQVAKSFNK LKNVCK LRSQ KDA +VYVDVYTIKYNLFAHP  
Sbjct: 247 HNKRDVDQIGCLRVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKT 306

Query: 301 YGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAI 360
           YGFE  LM CCGYGG PNNYNVKATCGQPGYS+CANPSK+IVWDGVH+++AANR+VASAI
Sbjct: 307 YGFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVASAI 366

Query: 361 LSAHFSTPQLTLRQLFL 375
           LS++FSTP LTL Q +L
Sbjct: 367 LSSNFSTPNLTLHQFWL 381

BLAST of CmoCh14G009710 vs. ExPASy TrEMBL
Match: A0A2I4G2K2 (GDSL esterase/lipase At3g62280 OS=Juglans regia OX=51240 GN=LOC109004139 PE=3 SV=2)

HSP 1 Score: 548.1 bits (1411), Expect = 2.8e-152
Identity = 257/353 (72.80%), Postives = 301/353 (85.27%), Query Frame = 0

Query: 21  WVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRF 80
           WVCV  L +F  S   +   P LINFGDSNSDTGGVLAGTGLPIGLPHGITFFH GTGR 
Sbjct: 15  WVCVF-LGMFANSKCSSNIRPILINFGDSNSDTGGVLAGTGLPIGLPHGITFFHSGTGRL 74

Query: 81  GDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQFI 140
           GDGRL+IDF CE L L YLSPYL+SL+PNFTSGVNFAVSGAT LPQFVPFALDVQ+RQFI
Sbjct: 75  GDGRLVIDFVCEHLNLSYLSPYLDSLSPNFTSGVNFAVSGATLLPQFVPFALDVQVRQFI 134

Query: 141 HFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFL 200
            F+ RSL L SLG ++N++DE+G +N +YMIDIG+NDLL+ALYA+NLTY PV +K+PSFL
Sbjct: 135 RFRKRSLELLSLG-SRNLIDEDGIRNALYMIDIGENDLLLALYATNLTYAPVIEKVPSFL 194

Query: 201 AEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFN 260
           AEIKLAIQN+Y  GGR+FWIHNTGPLGC+PKELALHPH + D+D+IGCLR+HN VAK+FN
Sbjct: 195 AEIKLAIQNIYQYGGRRFWIHNTGPLGCAPKELALHPHNNTDLDRIGCLRIHNDVAKAFN 254

Query: 261 KDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNVK 320
           + + NVCK+LRS FKDA +VYVD+YTIKY LFA    YGFE   +ACCGYGG PNNYNVK
Sbjct: 255 EGVNNVCKELRSVFKDATIVYVDIYTIKYKLFAKYKKYGFEDPFVACCGYGGPPNNYNVK 314

Query: 321 ATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSAHFSTPQLTLRQLF 374
           ATCGQPGYSIC N S++IVWDGVHY+EAANRV+A++ILS H++TP++ L Q +
Sbjct: 315 ATCGQPGYSICRNVSRSIVWDGVHYTEAANRVIATSILSTHYTTPRVKLDQFW 365

BLAST of CmoCh14G009710 vs. ExPASy TrEMBL
Match: A0A6J1A1L8 (GDSL esterase/lipase At3g62280 OS=Herrania umbratica OX=108875 GN=LOC110414272 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 4.5e-150
Identity = 257/353 (72.80%), Postives = 301/353 (85.27%), Query Frame = 0

Query: 21  WVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRF 80
           W+ +  LA+   S S     P +INFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGR 
Sbjct: 15  WIWIF-LAMTDISESSPAGRPRIINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRL 74

Query: 81  GDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQFI 140
           GDGRLIIDFFCE L L YLSPYL+SLAPNFTSGVNFAVSGA TLPQFVPFALDVQ+RQFI
Sbjct: 75  GDGRLIIDFFCERLNLSYLSPYLDSLAPNFTSGVNFAVSGAMTLPQFVPFALDVQVRQFI 134

Query: 141 HFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFL 200
            FKNRS+ LQ+ G   + +DE+GF++ +YMIDIGQNDLL+ALYASNLTY+PVA++IPSFL
Sbjct: 135 RFKNRSIELQTKG-LGDFIDEKGFRDALYMIDIGQNDLLMALYASNLTYEPVAEQIPSFL 194

Query: 201 AEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFN 260
           AEIKLAIQN+Y+ GGRKFWIHNTGPLGC+PKELALH HT+KD+D+IGC RVHN +AK+FN
Sbjct: 195 AEIKLAIQNIYSYGGRKFWIHNTGPLGCAPKELALHSHTNKDLDRIGCFRVHNDLAKAFN 254

Query: 261 KDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNVK 320
           K L+N+CK++R+  KDA +VY+DVYTIKYNLF     YGFE   MACCGYGG PNNY+ K
Sbjct: 255 KGLRNICKEMRTVLKDATIVYIDVYTIKYNLFTKYKKYGFEYPFMACCGYGGPPNNYDSK 314

Query: 321 ATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSAHFSTPQLTLRQLF 374
           ATCGQPG SIC N S++IVWDGVHYSEA+NRVVA+++LS ++STPQ+ L   +
Sbjct: 315 ATCGQPGSSICNNVSRSIVWDGVHYSEASNRVVATSVLSGYYSTPQMKLENFW 365

BLAST of CmoCh14G009710 vs. TAIR 10
Match: AT3G62280.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 483.4 bits (1243), Expect = 1.6e-136
Identity = 234/350 (66.86%), Postives = 279/350 (79.71%), Query Frame = 0

Query: 24  VISLAIFIPSTSCTP----TPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGR 83
           V+ L++ + S S T       P LINFGDSNSDTGGVLAG GLPIGLPHGITFFHRGTGR
Sbjct: 14  VLCLSLLVCSNSETSYKSNKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGR 73

Query: 84  FGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQF 143
            GDGRLI+DF+CE LK+ YLSPYL+SL+PNF  GVNFAVSGAT LP F  F L +QIRQF
Sbjct: 74  LGDGRLIVDFYCEHLKMTYLSPYLDSLSPNFKRGVNFAVSGATALPIF-SFPLAIQIRQF 133

Query: 144 IHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSF 203
           +HFKNRS  L S G+ ++++D+ GF+N +YMIDIGQNDLL+ALY SNLTY PV +KIPS 
Sbjct: 134 VHFKNRSQELISSGR-RDLIDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSM 193

Query: 204 LAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSF 263
           L EIK AIQ +Y  GGRKFW+HNTGPLGC+PKELA+H H   D+D IGC RVHN+VAK+F
Sbjct: 194 LLEIKKAIQTVYLYGGRKFWVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAF 253

Query: 264 NKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNV 323
           NK L ++C +LRSQFKDA +VYVD+Y+IKY L A   +YGF   LMACCGYGG PNNY+ 
Sbjct: 254 NKGLLSLCNELRSQFKDATLVYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDR 313

Query: 324 KATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSAHFSTPQLTL 370
           KATCGQPG +IC + +KAIVWDGVHY+EAANR V  A+L+  +S P+ +L
Sbjct: 314 KATCGQPGSTICRDVTKAIVWDGVHYTEAANRFVVDAVLTNRYSYPKNSL 361

BLAST of CmoCh14G009710 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 5.3e-103
Identity = 180/365 (49.32%), Postives = 247/365 (67.67%), Query Frame = 0

Query: 9   TTLMFDPRHNPFWVCVISLAIFI---PS--TSCTPTPPTLINFGDSNSDTGGVLAGTGLP 68
           T  ++    + F++ ++SLA+ I   PS   SCT   P + NFGDSNSDTGG++AG G P
Sbjct: 3   TLFLYSNTFSFFFITLVSLALLILRQPSRAASCT-ARPVIFNFGDSNSDTGGLVAGLGYP 62

Query: 69  IGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGAT 128
           IG P+G  FF R TGR  DGRL+IDF C+ L    L PYL+SL    F +G NFA++G+ 
Sbjct: 63  IGFPNGRLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSP 122

Query: 129 TLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKN--IMDEEGFKNGVYMIDIGQNDLLV 188
           TLP+ VPF+L++Q++QF HFK+RSL L S   +     +   GFKN +YMIDIGQND + 
Sbjct: 123 TLPKNVPFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQND-IA 182

Query: 189 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 248
             +A   +Y    + IP  + EIK +I+ LY  GGR+FWIHNTGPLGC P++L++     
Sbjct: 183 RSFARGNSYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKS 242

Query: 249 KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF 308
           KD+DQ GCL  +N  A  FN+ L ++C++LR++ +DA ++Y+D+Y IKY+L A+ + YGF
Sbjct: 243 KDLDQHGCLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGF 302

Query: 309 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANRVVASAILSA 366
           +  LMACCGYGG P NYNVK TCG  G ++C   S+ I WDG+HY+E AN +VA  +LS 
Sbjct: 303 KSPLMACCGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVAMKVLSM 362

BLAST of CmoCh14G009710 vs. TAIR 10
Match: AT1G09390.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 6.9e-103
Identity = 176/329 (53.50%), Postives = 238/329 (72.34%), Query Frame = 0

Query: 40  PPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYL 99
           PP + NFGDSNSDTGG++AG G  IGLP+G +FF R TGR  DGRL+IDF C+ L    L
Sbjct: 35  PPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLSDGRLVIDFLCQSLNTSLL 94

Query: 100 SPYLESL-APNFTSGVNFAVSGATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGK--TK 159
           +PYL+SL    F +G NFA+ G++TLP++VPFAL++Q+ QF+HFK+R+L L S+     +
Sbjct: 95  NPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQLMQFLHFKSRALELASISDPLKE 154

Query: 160 NIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGR 219
            ++ E GF+N +YMIDIGQND+  + ++  L+Y  V + IP+ ++EIK AI+ LY  GGR
Sbjct: 155 MMIGESGFRNALYMIDIGQNDIADS-FSKGLSYSRVVKLIPNVISEIKSAIKILYDEGGR 214

Query: 220 KFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKD 279
           KFW+HNTGPLGC P++L++  H+ K  D+ GCL  +N  AK FN+ L ++C+DLR++ K+
Sbjct: 215 KFWVHNTGPLGCLPQKLSM-VHS-KGFDKHGCLATYNAAAKLFNEGLDHMCRDLRTELKE 274

Query: 280 AIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSK 339
           A +VYVD+Y IKY+L A+ + YGFEK LMACCGYGG P NYNV  TCG  G   C   S+
Sbjct: 275 ANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEGSR 334

Query: 340 AIVWDGVHYSEAANRVVASAILSAHFSTP 366
            I WDG+HY+E AN +VA  +LS   STP
Sbjct: 335 FISWDGIHYTETANAIVAMKVLSMQHSTP 360

BLAST of CmoCh14G009710 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.3e-80
Identity = 164/373 (43.97%), Postives = 228/373 (61.13%), Query Frame = 0

Query: 22  VCVISLAIFIPST-SCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRF 81
           V  I +++F+PS+ S     P +INFGDSNSDTG +++     +  P+G T+F+  +GR+
Sbjct: 11  VLFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRY 70

Query: 82  GDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPFALDVQ 141
            DGRLI+DF  +++ L +L+PYL+SL  PNF  G NFA +G+T LP       PF+ D+Q
Sbjct: 71  CDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQ 130

Query: 142 IRQFIHFKNRSLHLQSLGKT-----KNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYK 201
           I QFI FK+R++ L  L KT     K +   + +  G+YMIDIGQND+  A Y+   T  
Sbjct: 131 ISQFIRFKSRAIEL--LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK--TLD 190

Query: 202 PVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLR 261
            V   IPS L   +  ++ LY  GGR  WIHNTGPLGC  + +A        +D+ GC+ 
Sbjct: 191 QVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVS 250

Query: 262 VHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGY 321
            HNQ AK FN  L  +    ++Q+ DA V YVD+++IK NL A+   +GFEK LMACCG 
Sbjct: 251 SHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGV 310

Query: 322 GGAPNNYNVKATCGQ----PGYSI----CANPSKAIVWDGVHYSEAANRVVASAILSAHF 376
           GGAP NY+ + TCGQ     G S+    C + S+ I WDG+HY+EAAN  V+S IL+  +
Sbjct: 311 GGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKY 370

BLAST of CmoCh14G009710 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.7e-80
Identity = 164/374 (43.85%), Postives = 224/374 (59.89%), Query Frame = 0

Query: 20  FWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGR 79
           F+V +  ++    S S     P+  NFGDSNSDTG ++AG G+ + LP+G   F   + R
Sbjct: 9   FYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 68

Query: 80  FGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPFALDV 139
           F DGRL+IDF  +++ L +L+PYL+SL  PNF  G NFA +G+T LP       PF+ D+
Sbjct: 69  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 128

Query: 140 QIRQFIHFKNRSLHLQSLGKT-----KNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 199
           QI QFI FK+R++ L  L KT     K +   + +  G+YMIDIGQND+  A Y+   T 
Sbjct: 129 QISQFIRFKSRAIEL--LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK--TL 188

Query: 200 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 259
             V   IPS L   +  ++ LY  GGR  WIHNTGPLGC  + +A        +D+ GC+
Sbjct: 189 DQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCV 248

Query: 260 RVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCG 319
             HNQ AK FN  L  +    ++Q+ DA V YVD+++IK NL A+   +GFEK LMACCG
Sbjct: 249 SSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCG 308

Query: 320 YGGAPNNYNVKATCGQ----PGYSI----CANPSKAIVWDGVHYSEAANRVVASAILSAH 376
            GGAP NY+ + TCGQ     G S+    C + S+ I WDG+HY+EAAN  V+S IL+  
Sbjct: 309 VGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGK 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NLP72.3e-13566.86GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2... [more]
Q9FXB67.5e-10249.32GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1[more]
O805229.8e-10253.50GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2... [more]
Q3ECP61.6e-7541.25GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2... [more]
Q9FXE52.1e-7243.75Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FA196.3e-221100.00GDSL esterase/lipase At3g62280-like OS=Cucurbita moschata OX=3662 GN=LOC11144219... [more]
A0A6J1J5S71.9e-21798.13GDSL esterase/lipase At3g62280-like OS=Cucurbita maxima OX=3661 GN=LOC111482013 ... [more]
A0A6J1DWR39.0e-18384.08GDSL esterase/lipase At3g62280 OS=Momordica charantia OX=3673 GN=LOC111023851 PE... [more]
A0A2I4G2K22.8e-15272.80GDSL esterase/lipase At3g62280 OS=Juglans regia OX=51240 GN=LOC109004139 PE=3 SV... [more]
A0A6J1A1L84.5e-15072.80GDSL esterase/lipase At3g62280 OS=Herrania umbratica OX=108875 GN=LOC110414272 P... [more]
Match NameE-valueIdentityDescription
AT3G62280.11.6e-13666.86GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G56670.15.3e-10349.32GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G09390.16.9e-10353.50GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.31.3e-8043.97GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.11.7e-8043.85GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 44..357
e-value: 4.7E-40
score: 138.4
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 35..363
e-value: 1.2E-68
score: 233.9
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 20..371
NoneNo IPR availablePANTHERPTHR22835:SF507BNAC06G18430D PROTEINcoord: 20..371
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 41..360
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 41..360
e-value: 6.16564E-113
score: 329.961

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh14G009710.1CmoCh14G009710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050896 response to stimulus
cellular_component GO:0016020 membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0016301 kinase activity