CmoCh14G005600 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh14G005600
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionHydroxymethylglutaryl-CoA lyase
LocationCmo_Chr14: 2838393 .. 2843435 (-)
RNA-Seq ExpressionCmoCh14G005600
SyntenyCmoCh14G005600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTGCTTTAGGACGATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCAGCTCCACTAACGGGCTTGGCATACAAGGAGAAGTTGATAATTGCTGGTATGGATGAAATGATTGTTCTTCACAAGTTCGTAATCCAGTATGTATGATCTGATTATAGGGAGAATTAATCATACCTTTTGATGCTTTCGTTTTTTTTATTAAATCGCTTATCGTTGCAATATCTTTTCGCTGAAAATGTGCGATAGAATTCTTTAAATCCTTATGGGGTGAGTCGTGGAATATGGATCGTGAAATTTGGGCGTTTTTCTTTTACGATTTTACGATGGGAAATCTACTTCGACGAATTTGGGGGAATCCGTTCAAGAGTGGAGCTAATAAGCTTTGGGTAAATGCAATCCAAACTATTATGTGGGTCTAGTTTGGAAGAAACAACATATTGTTCGGAGGGTACATGATTGGAGAGAATAATTTGATTAGTTTCAATCTTTGCTTAGCAATTAGGCCTTTGCAATTAATACAGATTAGGGCAGATTCTTGTCAGCTCTGGTGGGGAGTATGCTCTCTTGTATGATCACGGGTTGGTGTATTTATTTGTATAATGGGGCTTTGAAGTTCTAGTAACACGAAGAGGATTTAGACCACTTGTTTTGGGATTGTGATTTTATTACCTCTCTGTGGGATCAGTTTTTGCAAGACTTCTGGGGTTGCAAGCTCGTAACAGTGGGTGTTGTTCGATGTTTGAGGTGCTCTTGAACTCTCCTTTCCATGAAAAAGGAAGGGTTCTTCGGCTGCGGGGCATTTGGCTTGAGAGGAGCTCGAGTTTTTTGTGGGGTGGAGAGGTTTGGGGAGGATCTTTGGGATTTGATTAGATGTAATTCCTCTTTATGGGCAATATTTTTCTAATGCTGTTTACACAAATTAAGTTTGTACTTTGTAGTATTTATTGAAGATTTTCTTTAAGTCTCATGCTTAAGTTTGAATATAACACTGCATTTATGTCTCCACAGTCAACAAGATGGAGGAGATGTAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGGTGAAGTTCTAAAATGGCAGTTCCTAGTGAAATATACTATAATTGAAAAACTGTAGCTCCGATAACTATCGATCTGTCTACGAAAAAAAATCAACAAAGGTGTTTCAAATTTTGAAATGACTTTTTTCCTTTGTACTGCCATTGAGTTCTCTTTCCAGTCAAGATCTGTCTCATATGATTTGAAACAAGTTTTATTTTGGGTACTGTGTTCTTAATCTTAACTATTTGCAATGTTATTTTCAATTATAATGGTTATTTAAAATATTCAGCGGTTTCTGTCTCTTATGAAAAAAAGGAGAATAATAAATATATTGAATTTGTCTGATGAACGAATTTTTTCTGCCTTTGCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTGTTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGGTAACTTGGCTTGATAGTCAATGAACATGTTGGTTGGTATATTTTTGTGTTCCTAAAACAATTACTTCAACTTTTCATGCACTCTGCCTTCATGTTTGAGTTTCTTGTAGATATACTAATTAACCTCGTTTCCTCATATTTTCCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGTATGAACTTCAGTACAGAAATATAAAGGTAGAACTAATCTGGAATGCATTTTGTGATTATTGATGAGATTTTTTGACTGCTTTGTATAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGGTAGGTACTCTGAACCAGAAAACTGAGTTATTATATCATATCTGATATCTGGTTTGATTTCATATAAACATGTTAGATATGATTTGATACTCCCCTGGTTTGATATGATTTACTTTTTATATCTTGAAAATGTCTCCTATAAACATGTTAGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCCTACGTCGCTAAGGAGCTTTACGGCATGGGATGCTCTGAAATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGTAACCTCTTGGAATAGCTTTAGTTTAAAATCACAGTGGATATGAACAGAATCTATCTTCGGAAGGCATTTGGACAAACCGAAGAAAGATTATATCGCACGTTAAATTAGGTATCTGTATGGTCTATGCCTCTCATTCTAAATTGGTTAACTTATATCATTCATTGCTAGCATGTTGTAAGTACTCCATTATAGGATTTCCTTTGTATATCTTTTGTAATATTAGTTCTGTATTATGTGTATATTTCATATTTATACCGGTGTCTTCTTTATTTATTAATGGTTTTTCTTCTTATATAAGAGGACCTTGTATCTCCATAGTTTCTTAATTAGAAGAGTTCCCATGGTGAACTCAATTTTTCCTAAACAATTTTTTAACGATGATTTTTTTAAATATTTGTTTTAAGAATCCTTTTTTGACAGTAATTTTTCTAACTCTTCCAGGAGTCATTGGCAACATTCATGTTCTTGTTAACTTTAGTTTTCTCACCTCTGCAGGAACCGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACGTATGGGCAAGCTCTGTCCAATATCCTACTCTCACTCCAGGTAAACTCTGTTTTTTCGATCTTGGTTTTAATTGGTTGGAACCTACTAGTTTCAAAAAAATATATGCCAATGAGTTGGTATTTATTCGCGAGTATCAAAGTATAATTTAGGGAAATTAGAGACCTTATCTCTCCTGCTCTACCTTGCACATAGCGAGAATACTTGTTGTAATAGTCCAAGCCCATCGCTAGCAGATATTGTTCTTTTTGGGCTTTCCCTTTTGGGCTTCTCCTTAAGATTTTTAAAACGCGTCTACTAAGAAGAGATTTTCACACCCTTATAATGAATGTTTCGTTCTCCTCCCCAACCGACATGGGATCTCACTCTTATACCCAAACTCTTTCTGATGCCCTCTTTACTCCTAGGCAACCTGCTCACATAACTAACTTGCAGTGGCCCACTGTCCTATTATTCACGAAATTCAATTTTATGTTTCATAGATCTTTTGTTGAAAAGTTCCGGGACAAGATTTTCAATTTAACCAATAATATACTAGTAACTGATGTGTCAAATAATGAAGAGGAGTAGGTACTAACAACTAAAAATTTTCTCCTTTCACTTACTTCCTCTCTTGGCTTCTAAGACTATGTTGAATTTGATTAAGAGAAAAATATGTATGAAGTACTTAATTACAACATATATATATATACACGCGCTCATACATATATATCTAGTTTGTCAACTTAGAGAGCCCAAAAAGACAAAATGAAGACCGCGTTGTCCTAGAATTTTCAAACTTAGAAGAACTAGATTTTCTTAAAGAAGCATTATTCTAAGTTGAGGGCTACCGTGCTAAGATTTCATTTGATTTTGGTTTTTGAAAACCAAGCTTATGATTTTTTTCACTCTATATGATCAACTGTTGTAATGAAAACGAATCTGTTACATGCAATGGATATAATGATGTTGAAAACGATACAAAAGATGAAGAAGATGAAGAAAAACAGCTTATCTTATTAACAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGTCTAGGGGGCTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGACCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGAGGTACTTTACACTATATTCTTGCGTCTTCTCTGATATTGTTCCTCAAGACCCCTAGTAGCTTTCTTGTAAGTTTTAGGTGCAAAAGTATTTTATCATGTATGTTGCTCTCATATAACCCGAAACGAAAGCATTTATGAGTGAATAACCGAGCAACCTACAGAAAAAGGCCTCGAGTCGAGTAAAATAAGACCAAACACTTGAGCGGAATACGAGTCGAGGCCAAACCTCAAAGGGGTCTCTCAAACCCTCTGAATAATGAGCATGTTAGCCAATCCAATCATATTGACACAACTTATCATTCTGTTCAGGTGAAATGAACATTTCCTGTTCATCTTATCAAGGGCAATGTCTGTGAAGAAAACTCTTGCTTTCAATACTTAGTAGCATCGTGAATGCACAGAATGAAGCGTCGAAGAGATGCACGGTCTCGATGGCAGATGGGCATATGAAGAAATAATGGAGCACGGTCTCGTTAGTGTATTGATGAAAACCATTCATCTACACCGATCACCACCCTCTTTCATTAAACTTTCTCATGTTTCAATCTACAGCTTAACATTTCTCTATTTTTTTTTTTTTATCTCCTCTTCTTCAATTCTCCAATATTTAAAGCTACCTGTTCAATCAAAATATATAAATTATAGCAAGAAGAAAAAGAAAAGTATTGGAATGATTTAATACATTTGGATTTAATGCATGTGCATTACCAAAATAGTAGCTATGAGGATTTGTTGGCC

mRNA sequence

TATTGCTTTAGGACGATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCAGCTCCACTAACGGGCTTGGCATACAAGGAGAAGTTGATAATTGCTGTCAACAAGATGGAGGAGATGTAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTGTTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCCTACGTCGCTAAGGAGCTTTACGGCATGGGATGCTCTGAAATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAACCGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACGTATGGGCAAGCTCTGTCCAATATCCTACTCTCACTCCAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGTCTAGGGGGCTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGACCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGAGGTGAAATGAACATTTCCTGTTCATCTTATCAAGGGCAATGTCTGTGAAGAAAACTCTTGCTTTCAATACTTAGTAGCATCGTGAATGCACAGAATGAAGCGTCGAAGAGATGCACGGTCTCGATGGCAGATGGGCATATGAAGAAATAATGGAGCACGGTCTCGTTAGTGTATTGATGAAAACCATTCATCTACACCGATCACCACCCTCTTTCATTAAACTTTCTCATGTTTCAATCTACAGCTTAACATTTCTCTATTTTTTTTTTTTTATCTCCTCTTCTTCAATTCTCCAATATTTAAAGCTACCTGTTCAATCAAAATATATAAATTATAGCAAGAAGAAAAAGAAAAGTATTGGAATGATTTAATACATTTGGATTTAATGCATGTGCATTACCAAAATAGTAGCTATGAGGATTTGTTGGCC

Coding sequence (CDS)

ATGCAAGCTGCAAAGGTTTCTGCTAAATTGACTCGACGGGGTTTGTCTTGGCGCCATGGATATAATCCAACCATGAGAATTTCCAGCCGGTTTCTTAGTTCATCTTCGGCTTCGCATGTAGGCGTCAGCTCCACTAACGGGCTTGGCATACAAGGAGAAGTTGATAATTGCTGTCAACAAGATGGAGGAGATGTAGTTCTTTCAAGAGAAAATAAGAGGAGATTGTCCCAGTATTTACGCCATATTCCGCAGTTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCAACTGCTGTTAAGGTTGAATTAATAAAGATGCTAGTCTCTTCTGGGTTGCGTGTTGTTGAAGCCACGAGTTTTGTTTCTCCTAAATGGGTACCTCAGTTAGCAGATGCAAACGATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATATTAAGGGTTTTGAAGCGGCTGTTGCAGCTGGAGCAAAGGAAGTAGCTATCTTTGCTTCAGCATCCGAATCCTTCTCAAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTCCGTCGTTACCGTGAAGTTGCTGTTGCTGCAAGAAAGCACTCACTTCCAGTTCGCGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCCCCATCAAATGTAGCCTACGTCGCTAAGGAGCTTTACGGCATGGGATGCTCTGAAATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAACCGTGATTCCAATGCTTGAATCTGTAGTTGATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACGTATGGGCAAGCTCTGTCCAATATCCTACTCTCACTCCAGATGGGGATTGGCACGGTGGATTGTTCGGTTTCTGGTCTAGGGGGCTGCCCATATGCTAAAGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGAGTGAAGACCAACGTAGACCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTCCAAGCACCTGGGGCGTCCTTCCGCTTCAAAAGTAGCAATTGCATTGCAGAAAGTTGCCTCCAAGCCCAATAAATGCACAGCTACTGCATCCAAATTATGA

Protein sequence

MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQDGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIALQKVASKPNKCTATASKL
Homology
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BLAST of CmoCh14G005600 vs. ExPASy Swiss-Prot
Match: O81027 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HMGCL PE=1 SV=2)

HSP 1 Score: 475.7 bits (1223), Expect = 5.0e-133
Identity = 241/311 (77.49%), Postives = 274/311 (88.10%), Query Frame = 0 

Query: 82  IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 141
           IP+FVKIVEVGPRDGLQNEK+IVPT+VKVELI+ LVSSGL VVEATSFVSPKWVPQLADA
Sbjct: 164 IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSPKWVPQLADA 223

Query: 142 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 201
            DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAKEVAIFASASESFS SNINC+IEESL 
Sbjct: 224 KDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNINCTIEESLL 283

Query: 202 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 261
           RYR VA AA++HS+PVRGYVSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+
Sbjct: 284 RYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGI 343

Query: 262 GTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPY 321
           GTPG+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NIL+SLQMGI  VD S++GLGGCPY
Sbjct: 344 GTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSSIAGLGGCPY 403

Query: 322 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIALQKVASK 381
           AKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFISKHLGRP+ SK A+AL +    
Sbjct: 404 AKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAAVALNR---- 463

Query: 382 PNKCTATASKL 393
             + TA ASK+
Sbjct: 464 --RITADASKI 468

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BLAST of CmoCh14G005600 vs. ExPASy Swiss-Prot
Match: Q8JZS7 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hmgcll1 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.9e-108
Identity = 198/312 (63.46%), Postives = 242/312 (77.56%), Query Frame = 0 

Query: 63  GDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLR 122
           GD V    +  + +  L  +P++VKIVEVGPRDGLQNEK IVPT +K+ELI  L  +GL 
Sbjct: 25  GDSVAGALDAAQEASQLPGLPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLS 84

Query: 123 VVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFAS 182
           V+E TSFVS +WVPQ+AD  +VM GIR   G R+PVLTPN++GF+ AVAAGA E+A+F +
Sbjct: 85  VIEVTSFVSSRWVPQMADHAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGA 144

Query: 183 ASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVA 242
           ASESFSK NINCSIEES+ R++EV  +AR   +PVRGYVSC +GCP EG + P  V  V+
Sbjct: 145 ASESFSKKNINCSIEESMGRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVS 204

Query: 243 KELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSL 302
           K LYGMGC EISLGDTIGVGTPG++  MLESV+  +P   LAVH HDTYGQAL+NIL +L
Sbjct: 205 KRLYGMGCYEISLGDTIGVGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTAL 264

Query: 303 QMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKH 362
           QMGI  VD +VSGLGGCPYAKGASGNVATED++YMLNG+G+ T VDL K+M AG+FI K 
Sbjct: 265 QMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKA 324

Query: 363 LGRPSASKVAIA 375
           + + + SKVA A
Sbjct: 325 VNKTTNSKVAQA 336

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BLAST of CmoCh14G005600 vs. ExPASy Swiss-Prot
Match: P97519 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Hmgcl PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.6e-107
Identity = 196/297 (65.99%), Postives = 240/297 (80.81%), Query Frame = 0 

Query: 82  IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 141
           +P+ VKIVEVGPRDGLQNEK IVPT VK++LI ML  +GL V+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKRVKIVEVGPRDGLQNEKSIVPTPVKIKLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 142 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 201
           +DV++GI+   G  +PVLTPN+KGFE AVAAGAKEV+IF +ASE F++ N+NCSIEES +
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSIFGAASELFTRKNVNCSIEESFQ 148

Query: 202 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 261
           R+  V  AAR  S+ VRGYVSC +GCP EG V P+ VA VAK+LY MGC EISLGDTIGV
Sbjct: 149 RFDGVMQAARAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 262 GTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPY 321
           GTPG +  ML +V+  VP+  LAVH HDTYGQAL+N L++LQMG+  VD SV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVLHEVPVAALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 322 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIALQKV 379
           AKGASGN+ATED+VYML G+G+ T V+L+KL+ AGDFI + L R ++SKVA A  K+
Sbjct: 269 AKGASGNLATEDLVYMLTGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

[Close]
BLAST of CmoCh14G005600 vs. ExPASy Swiss-Prot
Match: P35915 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL PE=1 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 4.7e-107
Identity = 194/292 (66.44%), Postives = 236/292 (80.82%), Query Frame = 0 

Query: 83  PQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADAN 142
           PQ VK+VEVGPRDGLQNEK +VPT VK+ LI ML  +GL V+EATSFVSP+WVPQ+AD  
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 143 DVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLRR 202
           +VM+GI  + G  +PVLTPN+KGF+AAVAAGAKEV+IF +ASE F+K NINCSIEESL R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 203 YREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGVG 262
           + EV  AAR  S+PVRGYVSCV+GCP EG +  + VA V+K++Y MGC EISLGD IG+G
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 263 TPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPYA 322
           TPG++  ML +V+  VP+  LAVH HDTYGQAL+NIL++LQMG+  VD SV+GLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 323 KGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIA 375
           +GASGNVATED+VYMLNG+G+ T VDL+KLM  G FI   L R + SKV+ A
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQA 294

[Close]
BLAST of CmoCh14G005600 vs. ExPASy Swiss-Prot
Match: P38060 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl PE=1 SV=2)

HSP 1 Score: 387.1 bits (993), Expect = 2.3e-106
Identity = 194/297 (65.32%), Postives = 238/297 (80.13%), Query Frame = 0 

Query: 82  IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 141
           +P+ VKIVEVGPRDGLQNEK IVPT VK+ LI ML  +GL V+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 142 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 201
           +DV++GI+   G  +PVLTPN+KGFE AVAAGAKEV++F + SE F++ N NCSIEES +
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQ 148

Query: 202 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 261
           R+  V  AA+  S+ VRGYVSC +GCP EG V P+ VA VAK+LY MGC EISLGDTIGV
Sbjct: 149 RFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 262 GTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPY 321
           GTPG +  ML +V+  VP+  LAVH HDTYGQAL+N L++LQMG+  VD SV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 322 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIALQKV 379
           AKGASGN+ATED+VYMLNG+G+ T V+L+KL+ AGDFI + L R ++SKVA A  K+
Sbjct: 269 AKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

[Close]
BLAST of CmoCh14G005600 vs. ExPASy TrEMBL
Match: A0A6J1F7V0 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE=3 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 5.8e-217
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0 

Query: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 60
           MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ
Sbjct: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 60

Query: 61  DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120
           DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG
Sbjct: 61  DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 121 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180
           LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF
Sbjct: 121 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240
           ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240

Query: 241 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300
           VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL
Sbjct: 241 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300

Query: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360
           SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS
Sbjct: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360

Query: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 393
           KHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 392

[Close]
BLAST of CmoCh14G005600 vs. ExPASy TrEMBL
Match: A0A6J1J8Q9 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 6.0e-214
Identity = 387/392 (98.72%), Postives = 388/392 (98.98%), Query Frame = 0 

Query: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 60
           MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEV NCCQQ
Sbjct: 2   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVHNCCQQ 61

Query: 61  DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120
           DGGD VLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG
Sbjct: 62  DGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 121

Query: 121 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180
           LR+VEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF
Sbjct: 122 LRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 181

Query: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240
           ASASESFSKSNINCSIEESLRRYREVAVAARK SLPVRGYVSCVVGCPIEGMVPPSNVAY
Sbjct: 182 ASASESFSKSNINCSIEESLRRYREVAVAARKQSLPVRGYVSCVVGCPIEGMVPPSNVAY 241

Query: 241 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL
Sbjct: 242 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 301

Query: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360
           SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS
Sbjct: 302 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 361

Query: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 393
           KHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 362 KHLGRPSASKVAIALQKVASKPNKCTATASKL 393

[Close]
BLAST of CmoCh14G005600 vs. ExPASy TrEMBL
Match: A0A6J1F1Y8 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 7.2e-183
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0 

Query: 59  QQDGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 118
           QQDGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS
Sbjct: 14  QQDGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 73

Query: 119 SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 178
           SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA
Sbjct: 74  SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 133

Query: 179 IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV 238
           IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV
Sbjct: 134 IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV 193

Query: 239 AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNI 298
           AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNI
Sbjct: 194 AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNI 253

Query: 299 LLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF 358
           LLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF
Sbjct: 254 LLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF 313

Query: 359 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 393
           ISKHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 314 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 347

[Close]
BLAST of CmoCh14G005600 vs. ExPASy TrEMBL
Match: A0A6J1J6X5 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.1e-180
Identity = 330/334 (98.80%), Postives = 331/334 (99.10%), Query Frame = 0 

Query: 59  QQDGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 118
           QQDGGD VLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS
Sbjct: 14  QQDGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVS 73

Query: 119 SGLRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 178
           SGLR+VEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA
Sbjct: 74  SGLRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVA 133

Query: 179 IFASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNV 238
           IFASASESFSKSNINCSIEESLRRYREVAVAARK SLPVRGYVSCVVGCPIEGMVPPSNV
Sbjct: 134 IFASASESFSKSNINCSIEESLRRYREVAVAARKQSLPVRGYVSCVVGCPIEGMVPPSNV 193

Query: 239 AYVAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNI 298
           AYVAKELY MGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNI
Sbjct: 194 AYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNI 253

Query: 299 LLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF 358
           LLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF
Sbjct: 254 LLSLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDF 313

Query: 359 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 393
           ISKHLGRPSASKVAIALQKVASKPNKCTATASKL
Sbjct: 314 ISKHLGRPSASKVAIALQKVASKPNKCTATASKL 347

[Close]
BLAST of CmoCh14G005600 vs. ExPASy TrEMBL
Match: A0A0A0LHM6 (Hydroxymethylglutaryl-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 2.0e-180
Identity = 338/392 (86.22%), Postives = 350/392 (89.29%), Query Frame = 0 

Query: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 60
           MQAAK+SAK TR+  SWRH YN +M   + FL S SA+ VGVSS    GIQ E  NC QQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 61  DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120
           DG D VLSRENK  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 121 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180
           L VVEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240
           ASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESV +VVP ENLAVHFHDTYGQALSNIL+
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360
           SLQMGIGTVD SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFI 
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360

Query: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 393
           K LGRPSASKVAIAL+KVAS  NKCTATASKL
Sbjct: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392

[Close]
BLAST of CmoCh14G005600 vs. TAIR 10
Match: AT2G26800.1 (Aldolase superfamily protein )

HSP 1 Score: 475.7 bits (1223), Expect = 3.6e-134
Identity = 241/311 (77.49%), Postives = 274/311 (88.10%), Query Frame = 0 

Query: 82  IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 141
           IP+FVKIVEVGPRDGLQNEK+IVPT+VKVELI+ LVSSGL VVEATSFVSPKWVPQLADA
Sbjct: 129 IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSPKWVPQLADA 188

Query: 142 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 201
            DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAKEVAIFASASESFS SNINC+IEESL 
Sbjct: 189 KDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNINCTIEESLL 248

Query: 202 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 261
           RYR VA AA++HS+PVRGYVSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+
Sbjct: 249 RYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGI 308

Query: 262 GTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPY 321
           GTPG+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NIL+SLQMGI  VD S++GLGGCPY
Sbjct: 309 GTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSSIAGLGGCPY 368

Query: 322 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIALQKVASK 381
           AKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFISKHLGRP+ SK A+AL +    
Sbjct: 369 AKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAAVALNR---- 428

Query: 382 PNKCTATASKL 393
             + TA ASK+
Sbjct: 429 --RITADASKI 433

[Close]
BLAST of CmoCh14G005600 vs. TAIR 10
Match: AT2G26800.2 (Aldolase superfamily protein )

HSP 1 Score: 475.7 bits (1223), Expect = 3.6e-134
Identity = 241/311 (77.49%), Postives = 274/311 (88.10%), Query Frame = 0 

Query: 82  IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 141
           IP+FVKIVEVGPRDGLQNEK+IVPT+VKVELI+ LVSSGL VVEATSFVSPKWVPQLADA
Sbjct: 164 IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSPKWVPQLADA 223

Query: 142 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 201
            DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAKEVAIFASASESFS SNINC+IEESL 
Sbjct: 224 KDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNINCTIEESLL 283

Query: 202 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 261
           RYR VA AA++HS+PVRGYVSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+
Sbjct: 284 RYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGI 343

Query: 262 GTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILLSLQMGIGTVDCSVSGLGGCPY 321
           GTPG+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NIL+SLQMGI  VD S++GLGGCPY
Sbjct: 344 GTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSSIAGLGGCPY 403

Query: 322 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFISKHLGRPSASKVAIALQKVASK 381
           AKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFISKHLGRP+ SK A+AL +    
Sbjct: 404 AKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAAVALNR---- 463

Query: 382 PNKCTATASKL 393
             + TA ASK+
Sbjct: 464 --RITADASKI 468

[Close]
BLAST of CmoCh14G005600 vs. TAIR 10
Match: AT2G26800.3 (Aldolase superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.2e-81
Identity = 150/187 (80.21%), Postives = 168/187 (89.84%), Query Frame = 0 

Query: 82  IPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSGLRVVEATSFVSPKWVPQLADA 141
           IP+FVKIVEVGPRDGLQNEK+IVPT+VKVELI+ LVSSGL VVEATSFVSPKWVPQLADA
Sbjct: 164 IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSPKWVPQLADA 223

Query: 142 NDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLR 201
            DVM+ +  ++GAR PVLTPN+KGF+AAV+AGAKEVAIFASASESFS SNINC+IEESL 
Sbjct: 224 KDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNINCTIEESLL 283

Query: 202 RYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAYVAKELYGMGCSEISLGDTIGV 261
           RYR VA AA++HS+PVRGYVSCVVGCP+EG V PS VAYV KELY MGC EISLGDTIG+
Sbjct: 284 RYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFEISLGDTIGI 343

Query: 262 GTPGTVI 269
           GTPG  +
Sbjct: 344 GTPGNFV 350

The following BLAST results are available for this feature:
BLAST of CmoCh14G005600 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O810275.0e-13377.49Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 G... [more]
Q8JZS71.9e-10863.463-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hm... [more]
P975193.6e-10765.99Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=... [more]
P359154.7e-10766.44Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL... [more]
P380602.3e-10665.32Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl... [more]
BLAST of CmoCh14G005600 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1F7V05.8e-217100.00Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE... [more]
A0A6J1J8Q96.0e-21498.72Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3... [more]
A0A6J1F1Y87.2e-183100.00Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE... [more]
A0A6J1J6X51.1e-18098.80Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3... [more]
A0A0A0LHM62.0e-18086.22Hydroxymethylglutaryl-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=3 ... [more]
BLAST of CmoCh14G005600 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G26800.13.6e-13477.49Aldolase superfamily protein [more]
AT2G26800.23.6e-13477.49Aldolase superfamily protein [more]
AT2G26800.31.2e-8180.21Aldolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000891Pyruvate carboxyltransferasePFAMPF00682HMGL-likecoord: 86..358
e-value: 3.4E-56
score: 190.7
IPR000891Pyruvate carboxyltransferasePROSITEPS50991PYR_CTcoord: 86..353
score: 35.049644
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 79..378
e-value: 2.0E-114
score: 383.9
NoneNo IPR availablePANTHERPTHR42738:SF15HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 69..381
NoneNo IPR availableCDDcd07938DRE_TIM_HMGLcoord: 88..361
e-value: 2.03729E-174
score: 484.974
NoneNo IPR availableSUPERFAMILY51569Aldolasecoord: 81..375
IPR043594HMG-CoA lyasePANTHERPTHR42738HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 69..381

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh14G005600.1CmoCh14G005600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046951 ketone body biosynthetic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004419 hydroxymethylglutaryl-CoA lyase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016833 oxo-acid-lyase activity