CmoCh14G005180 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh14G005180
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	ribosomal RNA small subunit methyltransferase NEP1
Location	Cmo_Chr14: 2608372 .. 2610580 (-)
RNA-Seq Expression	CmoCh14G005180
Synteny	CmoCh14G005180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCGGCTACAACCCTCTCTTCGCGCCTACTGTTAAGCGAGTTCGAGCTCAAAGAAGAACAATGGTTCGACCGTTTGCGGCGAAAGGGAAGAAGAGAAAGAAGGCGGAAAGATACGACAGAGACGATGATGGCGAAGAATCGACCGCTTCGGCCAAGAAGGCTATGGTGGAGAACGAACCTGATGAACAACCAGAGAATGAAGCAGCAGAAGAAAACTTCCACGAGCTTGAAGGTATTCCAATCGTTCCGAAAGACCCCAAGAGTGATAGTAAGGCTGGTGTCATCTTCGTCCTCGAACGAGCTTCTCTGGAGGTCGCCAAAGTTGGGAAGGTAGGTATTCTCTTTTTTATGCTTCGTTTTTTGGTTTGCTGCTTTGGAGTTTTAGGATTTAATTCGATTATGGCGTTTAACTGAGCTTTTTTATGCGTTTTTGAAGTTTGAACTTACTATTTACTTTCCATTCTCTGTTTTTATAAACTGGATTTCAATTTTTTTTTGTTTTAGAATTACCAGCTGTTGAATTCCGACGACCACTCCAATTTTTTGCGGAGAAACAATAGAAATCCTGGGGATTACAGACCTGACATCCTGCATCAAGTAAGCAGCTGTAGCTCACTGTTGTTTTTCCAATTGGATAGGAGAGAGCGAATTGTTTACGTTGATATTTGAGTACCATAAGTGATATTAAATATGAATGATTCAGGCTCTCCTGGCGATTTTCGATAGCCGCATTGCAAAGGCTGGGAGGTTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATCAAACCATATGTTCGTCTTCCAAGGACGCAGAAGCGGTTTTATGGTGTTATGTGTAAGTTGATTTTGTTGTTCTAATTTGGCTGAACATCTATCGAATTGGAGTCTGTTTTGAATCCTTTTCATTGGCGTTTTGCCAAATACTTTGCAGTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGCAAGCGTGAGAAGCTTTTCAGGGTGATAAAAAACCCTGTAACACAGTATTTACCTTCCAATTCTCGTAAAATGGGTAAGCCTATATAATGCTGTTTCCCCATTGTGAGTTTTGGGTTTTGGTAGAAACTTTTCATACTGTTTTCTTGCATATCAGGCTTCTCGCATAGTTCAGACAAGTTGGTCAAGGTGCGAACTTATCTGGATGCTGTCGAAGATGATGTAGACCTTGTTTTTGTGGTATCTTTAAACTCCCTACCGGAAATTTCACTTTTGCATGCTTCATTGTTATGTTTATACATATGACAACTCCCCCTTACTTCCCTTTACTGCAATGTGTATTAGGTGGGTGCAATGGCTCATGGGAAAATTGAGACCGGTTACACCGATGATCTTTTAGCAAGTAAGTTCGTTTTCTTTCGTGTTTATTTCAAGTCATTGGATAGGAGGAAATGGCTACATTAGTACATTTACTTTTAACTTCAGAAGATTTTTGAAAGATGAAGTTTTAATATCATTTACTTTGAAGGACGGCTTCTAAATTGTAGCCAAACATAAGCATTGAGGTAGAAGGGCTTCTTAAGTTTTGACCATTTGAAAGCACTTCCAAAGATATCATAGAGTGGTTTAAGCATTATATTTGTAAAAAATTGACTAGTTGGCAAATTGCTCGAACTTTTACTGTTATTACTGGGCATTATTACCTTGACTCTGTTTCTTATCCGAAAAGAAAAGAGAAGAAAAGTCGATTATCTTGGCCGTGCAATCCTGAATTGTTTGACGAATTTTCGTGTTATATTTGTGCAGTTTCTGAATATCCTCTCAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGATAAATGGAATGTAGGGTGAACCGTACCTACGACTCTGCATCAGTTAAGTTTGATAATCAGTGTCTCAACTCTGTTGTTTCATATTTATTAAGTTATTATTTCAGAGAATCTGCCTGCACAGATGTCTTAGGCGTTTATTTTTTCCTGCTTAAAAAATGAGCTGTAGAACGAATTAAACCTTACCATAGCTATTTCTGTGTAGCGAATTCTTAGTCGATTATGTTACAAATGCATCTTCTTCTAAATATAATTGTAAGCAGTTGATTTCTAGTTCTTAAAAGTTCGATCAGAAGTTATTGTACTGGAATTTTATTGCATAGCGTTTGAGTTCTTTGGTGTGCGTTAGAATATCAATTGTGAAA

mRNA sequence

TTCGGCTACAACCCTCTCTTCGCGCCTACTGTTAAGCGAGTTCGAGCTCAAAGAAGAACAATGGTTCGACCGTTTGCGGCGAAAGGGAAGAAGAGAAAGAAGGCGGAAAGATACGACAGAGACGATGATGGCGAAGAATCGACCGCTTCGGCCAAGAAGGCTATGGTGGAGAACGAACCTGATGAACAACCAGAGAATGAAGCAGCAGAAGAAAACTTCCACGAGCTTGAAGGTATTCCAATCGTTCCGAAAGACCCCAAGAGTGATAGTAAGGCTGGTGTCATCTTCGTCCTCGAACGAGCTTCTCTGGAGGTCGCCAAAGTTGGGAAGGATTTAATTCGATTATGGCGTTTAACTGAGCTTTTTTATGCGTTTTTGAAGTTTGAACTTACTATTTACTTTCCATTCTCTAATTACCAGCTGTTGAATTCCGACGACCACTCCAATTTTTTGCGGAGAAACAATAGAAATCCTGGGGATTACAGACCTGACATCCTGCATCAAGCTCTCCTGGCGATTTTCGATAGCCGCATTGCAAAGGCTGGGAGGTTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATCAAACCATATGTTCGTCTTCCAAGGACGCAGAAGCGGTTTTATGTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGCAAGCGTGAGAAGCTTTTCAGGGTGATAAAAAACCCTGTAACACAGTATTTACCTTCCAATTCTCGTAAAATGGGCTTCTCGCATAGTTCAGACAAGTTGGTCAAGGTGCGAACTTATCTGGATGCTGTCGAAGATGATGTAGACCTTGTTTTTGTGGTGGGTGCAATGGCTCATGGGAAAATTGAGACCGGTTACACCGATGATCTTTTAGCAATTTCTGAATATCCTCTCAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGATAAATGGAATGTAGGGTGAACCGTACCTACGACTCTGCATCAGTTAAGTTTGATAATCAGTGTCTCAACTCTGTTGTTTCATATTTATTAAGTTATTATTTCAGAGAATCTGCCTGCACAGATGTCTTAGGCGTTTATTTTTTCCTGCTTAAAAAATGAGCTGTAGAACGAATTAAACCTTACCATAGCTATTTCTGTGTAGCGAATTCTTAGTCGATTATGTTACAAATGCATCTTCTTCTAAATATAATTGTAAGCAGTTGATTTCTAGTTCTTAAAAGTTCGATCAGAAGTTATTGTACTGGAATTTTATTGCATAGCGTTTGAGTTCTTTGGTGTGCGTTAGAATATCAATTGTGAAA

Coding sequence (CDS)

ATGGTTCGACCGTTTGCGGCGAAAGGGAAGAAGAGAAAGAAGGCGGAAAGATACGACAGAGACGATGATGGCGAAGAATCGACCGCTTCGGCCAAGAAGGCTATGGTGGAGAACGAACCTGATGAACAACCAGAGAATGAAGCAGCAGAAGAAAACTTCCACGAGCTTGAAGGTATTCCAATCGTTCCGAAAGACCCCAAGAGTGATAGTAAGGCTGGTGTCATCTTCGTCCTCGAACGAGCTTCTCTGGAGGTCGCCAAAGTTGGGAAGGATTTAATTCGATTATGGCGTTTAACTGAGCTTTTTTATGCGTTTTTGAAGTTTGAACTTACTATTTACTTTCCATTCTCTAATTACCAGCTGTTGAATTCCGACGACCACTCCAATTTTTTGCGGAGAAACAATAGAAATCCTGGGGATTACAGACCTGACATCCTGCATCAAGCTCTCCTGGCGATTTTCGATAGCCGCATTGCAAAGGCTGGGAGGTTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATCAAACCATATGTTCGTCTTCCAAGGACGCAGAAGCGGTTTTATGTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGCAAGCGTGAGAAGCTTTTCAGGGTGATAAAAAACCCTGTAACACAGTATTTACCTTCCAATTCTCGTAAAATGGGCTTCTCGCATAGTTCAGACAAGTTGGTCAAGGTGCGAACTTATCTGGATGCTGTCGAAGATGATGTAGACCTTGTTTTTGTGGTGGGTGCAATGGCTCATGGGAAAATTGAGACCGGTTACACCGATGATCTTTTAGCAATTTCTGAATATCCTCTCAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGATAAATGGAATGTAGGGTGA

Protein sequence

MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIPIVPKDPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQLLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYVQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG

Homology

[Close]

BLAST of CmoCh14G005180 vs. ExPASy Swiss-Prot
Match: Q06287 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EMG1 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 9.5e-39
Identity = 86/189 (45.50%), Postives = 120/189 (63.49%), Query Frame = 0


Query: 119 YQLLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIE 178
           Y LLN DDH   L++  R+  + RPDI HQ LL + DS I KAG+L+ VY++T +G+LIE
Sbjct: 65  YVLLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSPINKAGKLQ-VYIQTSRGILIE 124

Query: 179 IKPYVRLPRTQKRF---YVQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHS 238
           + P VR+PRT KRF    VQLL KLSI +    EKL +VIKNP+T +LP+  RK+  S  
Sbjct: 125 VNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTLSFD 184

Query: 239 SDKLVKVRTYLDAVEDDVDLVFVVGAMAHGK--IETGYTDDLLAISEYPLSASCCIADIC 298
           +  +++V+ Y++ ++DD  +   VGAMA GK      Y D+ + +S YPLSAS   +  C
Sbjct: 185 A-PVIRVQDYIEKLDDDESICVFVGAMARGKDNFADEYVDEKVGLSNYPLSASVACSKFC 244

Query: 299 KDLADKWNV 303
               D WN+
Sbjct: 245 HGAEDAWNI 251

[Close]

BLAST of CmoCh14G005180 vs. ExPASy Swiss-Prot
Match: O35130 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=Emg1 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.6e-38
Identity = 99/239 (41.42%), Postives = 134/239 (56.07%), Query Frame = 0


Query: 70  SKAG---VIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQLLNSDD 129
           SK G   +I VLE ASLE  KVGK                            Y+LLN D 
Sbjct: 36  SKCGGRRLIVVLEGASLETVKVGK---------------------------TYELLNCDR 95

Query: 130 HSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLP 189
           H + L +N R+PG+ RPDI HQ+LL + DS + +AG L+ VY+ T+K +LIE+ P  R+P
Sbjct: 96  HKSMLLKNGRDPGEVRPDITHQSLLMLMDSPLNRAGLLQ-VYIHTQKNVLIEVNPQTRIP 155

Query: 190 RTQKRF---YVQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVR 249
           RT  RF    VQLL KLS+ AA   +KL +VIKNPV+ + P    K+G S S + +  +R
Sbjct: 156 RTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKIGTSFSVEDISDIR 215

Query: 250 TYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNV 303
              + V     +VFVVGA AHGK+   YT+ +++IS YPLSA+   A +     + W V
Sbjct: 216 ---ELVPSSDPVVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKVTTAFEEVWGV 243

[Close]

BLAST of CmoCh14G005180 vs. ExPASy Swiss-Prot
Match: Q92979 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EMG1 PE=1 SV=4)

HSP 1 Score: 161.0 bits (406), Expect = 2.1e-38
Identity = 105/268 (39.18%), Postives = 146/268 (54.48%), Query Frame = 0


Query: 43  QPENEAAEENFHELEGIPIVPKDPK--SDSKAG---VIFVLERASLEVAKVGKDLIRLWR 102
           +P   +  E   + + +P  PK P+  + +K G   +I VLE ASLE  KVGK       
Sbjct: 9   KPRERSGGEQAQDWDALP--PKRPRLGAGNKIGGRRLIVVLEGASLETVKVGK------- 68

Query: 103 LTELFYAFLKFELTIYFPFSNYQLLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSR 162
                                Y+LLN D H + L +N R+PG+ RPDI HQ+LL + DS 
Sbjct: 69  --------------------TYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSP 128

Query: 163 IAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRF---YVQLLQKLSITAAGKREKLFRV 222
           + +AG L+ VY+ T+K +LIE+ P  R+PRT  RF    VQLL KLS+ AA   +KL +V
Sbjct: 129 LNRAGLLQ-VYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKV 188

Query: 223 IKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDD 282
           IKNPV+ + P    K+G S S   +  VR   + V     +VFVVGA AHGK+   YT+ 
Sbjct: 189 IKNPVSDHFPVGCMKVGTSFSIPVVSDVR---ELVPSSDPIVFVVGAFAHGKVSVEYTEK 243

Query: 283 LLAISEYPLSASCCIADICKDLADKWNV 303
           +++IS YPLSA+   A +     + W V
Sbjct: 249 MVSISNYPLSAALTCAKLTTAFEEVWGV 243

[Close]

BLAST of CmoCh14G005180 vs. ExPASy Swiss-Prot
Match: Q10107 (Ribosomal RNA small subunit methyltransferase mra1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mra1 PE=1 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 8.0e-38
Identity = 99/230 (43.04%), Postives = 129/230 (56.09%), Query Frame = 0


Query: 74  VIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQLLNSDDHSNFLRR 133
           +I VL++A LE+ KVGK                          + YQLLN DDH   L++
Sbjct: 150 LIVVLDQACLEIYKVGK-----------------------AKDAKYQLLNCDDHQGILKK 209

Query: 134 NNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRF- 193
            NRN    RPDI HQ LL + DS + KAGRL+ VY+ T K +LIE+ P VR+PRT KRF 
Sbjct: 210 LNRNIAQARPDITHQCLLTLLDSPLNKAGRLQ-VYIHTAKKVLIEVNPSVRIPRTFKRFS 269

Query: 194 --YVQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVE 253
              VQLL KLSI +    EKL +VIKNPVT YLP N RK   S  +   V  R YL+ ++
Sbjct: 270 GLMVQLLHKLSIRSVNGNEKLLKVIKNPVTDYLPPNCRKATLSFDA-PTVPPRKYLETLQ 329

Query: 254 DDVDLVFVVGAMAHG--KIETGYTDDLLAISEYPLSASCCIADICKDLAD 299
            +  +   +GAMAHG      G+ D+ ++IS+YPLSAS   +     + D
Sbjct: 330 PNQSVCIAIGAMAHGPDDFSDGWVDEKISISDYPLSASIACSKFLHSMED 354

[Close]

BLAST of CmoCh14G005180 vs. ExPASy Swiss-Prot
Match: Q9XX15 (Ribosomal RNA small subunit methyltransferase nep-1 OS=Caenorhabditis elegans OX=6239 GN=Y39A1A.14 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.0e-37
Identity = 97/241 (40.25%), Postives = 132/241 (54.77%), Query Frame = 0


Query: 65  DPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQLLNS 124
           D + + K  +  VLE  SLE AKVG                             Y +L+S
Sbjct: 19  DNQLEDKKILYVVLEGCSLETAKVG---------------------------GEYAILSS 78

Query: 125 DDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVR 184
           D H+NFLR+  ++P DYRPDILHQ LL + DS + +AG+L+ V+ +T K +L+++ P  R
Sbjct: 79  DKHANFLRKQKKDPADYRPDILHQCLLNLLDSPLNRAGKLR-VFFRTSKNVLVDVSPQCR 138

Query: 185 LPRTQKRF---YVQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVK 244
           +PRT  RF    VQLL KLSI AA   +KL  V+KNPV+ +LP  SRKM  S +  +L  
Sbjct: 139 IPRTFDRFCGLMVQLLHKLSIRAAETTQKLMSVVKNPVSNHLPVGSRKMLMSFNVPELT- 198

Query: 245 VRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLADKWN 303
           +   L A E D  LV ++G +A GKI   Y D    IS YPLSA+   A +   L + W 
Sbjct: 199 MANKLVAPETDEPLVLIIGGIARGKIVVDYNDSETKISNYPLSAALTCAKVTSGLEEIWG 230

[Close]

BLAST of CmoCh14G005180 vs. ExPASy TrEMBL
Match: A0A6J1F634 (ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita moschata OX=3662 GN=LOC111442430 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.6e-143
Identity = 275/306 (89.87%), Postives = 276/306 (90.20%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60
           MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQ 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGK                           NYQ
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGK---------------------------NYQ 120

Query: 121 LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180
           LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK
Sbjct: 121 LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180

Query: 181 PYVRLPRTQKRFY---VQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD 240
           PYVRLPRTQKRFY   +QLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD
Sbjct: 181 PYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD 240

Query: 241 KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA 300
           KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA
Sbjct: 241 KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA 279

Query: 301 DKWNVG 304
           DKWNVG
Sbjct: 301 DKWNVG 279

[Close]

BLAST of CmoCh14G005180 vs. ExPASy TrEMBL
Match: A0A6J1J0G6 (ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita maxima OX=3661 GN=LOC111482341 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 8.1e-142
Identity = 273/306 (89.22%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60
           MVRPFAAKGKKRKK ERYDRDDDGEES ASAKKAMVENEPDEQPENEAAEENFHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKTERYDRDDDGEESIASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQ 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGK                           NYQ
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGK---------------------------NYQ 120

Query: 121 LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180
           LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK
Sbjct: 121 LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180

Query: 181 PYVRLPRTQKRFY---VQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD 240
           PYVRLPRTQKRFY   +QLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD
Sbjct: 181 PYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD 240

Query: 241 KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA 300
           KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA
Sbjct: 241 KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA 279

Query: 301 DKWNVG 304
           DKWNVG
Sbjct: 301 DKWNVG 279

[Close]

BLAST of CmoCh14G005180 vs. ExPASy TrEMBL
Match: A0A5D3DPQ6 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006800 PE=3 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.7e-129
Identity = 249/306 (81.37%), Postives = 265/306 (86.60%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60
           MVRPFAAKGKKRKKAE+YDRD+DGEES++ AKK MVENEPDE+P     +E+FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEP----PKEDFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQ 120
           IVPKDPK+D+ AGVIF+LERASLEVAKVGK                           NYQ
Sbjct: 61  IVPKDPKTDNNAGVIFILERASLEVAKVGK---------------------------NYQ 120

Query: 121 LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180
           LLNSDDHSN+LRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK
Sbjct: 121 LLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180

Query: 181 PYVRLPRTQKRFY---VQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD 240
           PYVRLPRTQKRFY   +QLLQKLSITAAGKREKLFRVIKNPVTQYLP+N RKMGFSHSSD
Sbjct: 181 PYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSSD 240

Query: 241 KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA 300
           KLVK+R YLDAV+DDVDLVFVVGAMAHGKIET YTDDLLAISEYPLSASCCIADICKDLA
Sbjct: 241 KLVKMRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDLA 275

Query: 301 DKWNVG 304
           +KWNVG
Sbjct: 301 EKWNVG 275

[Close]

BLAST of CmoCh14G005180 vs. ExPASy TrEMBL
Match: A0A1S3B7L0 (ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo OX=3656 GN=LOC103486891 PE=3 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.7e-129
Identity = 249/306 (81.37%), Postives = 265/306 (86.60%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60
           MVRPFAAKGKKRKKAE+YDRD+DGEES++ AKK MVENEPDE+P     +E+FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEP----PKEDFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQ 120
           IVPKDPK+D+ AGVIF+LERASLEVAKVGK                           NYQ
Sbjct: 61  IVPKDPKTDNNAGVIFILERASLEVAKVGK---------------------------NYQ 120

Query: 121 LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180
           LLNSDDHSN+LRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK
Sbjct: 121 LLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180

Query: 181 PYVRLPRTQKRFY---VQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD 240
           PYVRLPRTQKRFY   +QLLQKLSITAAGKREKLFRVIKNPVTQYLP+N RKMGFSHSSD
Sbjct: 181 PYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSSD 240

Query: 241 KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA 300
           KLVK+R YLDAV+DDVDLVFVVGAMAHGKIET YTDDLLAISEYPLSASCCIADICKDLA
Sbjct: 241 KLVKMRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDLA 275

Query: 301 DKWNVG 304
           +KWNVG
Sbjct: 301 EKWNVG 275

[Close]

BLAST of CmoCh14G005180 vs. ExPASy TrEMBL
Match: A0A0A0LFI3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G821550 PE=3 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 7.9e-129
Identity = 248/306 (81.05%), Postives = 264/306 (86.27%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60
           MVRPFAAKGKKRKK+E+YDRD+D EEST+ +KK M+ENEPDE+P    A+E+FHELEGIP
Sbjct: 33  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEP----AKEDFHELEGIP 92

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELFYAFLKFELTIYFPFSNYQ 120
           I PKDPK+DS AGVIF+LERASLEVAKVGK                           NYQ
Sbjct: 93  IAPKDPKNDSNAGVIFILERASLEVAKVGK---------------------------NYQ 152

Query: 121 LLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 180
           LLNSDDHSN+LRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK
Sbjct: 153 LLNSDDHSNYLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIK 212

Query: 181 PYVRLPRTQKRFY---VQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSD 240
           PYVRLPRTQKRFY   +QLLQKLSITAAGKREKLFRVIKNPVTQYLP+N RKMGFSHSSD
Sbjct: 213 PYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPANCRKMGFSHSSD 272

Query: 241 KLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLA 300
           KLVKVR YLDAV+DDVDLVFVVGAMAHGKIET YTDDLLAISEYPLSASCCIADICKDLA
Sbjct: 273 KLVKVRNYLDAVKDDVDLVFVVGAMAHGKIETDYTDDLLAISEYPLSASCCIADICKDLA 307

Query: 301 DKWNVG 304
           +KWNVG
Sbjct: 333 EKWNVG 307

[Close]

BLAST of CmoCh14G005180 vs. TAIR 10
Match: AT3G57000.1 (nucleolar essential protein-related )

HSP 1 Score: 278.9 bits (712), Expect = 4.9e-75
Identity = 165/325 (50.77%), Postives = 208/325 (64.00%), Query Frame = 0


Query: 1   MVRPFAAKGKKRK-KAERYDRDDD-------------GEESTASAKKAMVENEPDEQPEN 60
           MVRP+  K  KRK + ERYD+++D               ES+  AKK       ++  E 
Sbjct: 1   MVRPYGIKVNKRKEREERYDKEEDEVEEQPKFEQKQKARESSKKAKKESTSRAEEDNDEE 60

Query: 61  EAAEENFHELE----GIPIVPKDPKSDSKAGVIFVLERASLEVAKVGKDLIRLWRLTELF 120
           E   E     E    GIPIV   P  + K+G++FVLE+ASLEVAKVGK            
Sbjct: 61  EVTVEATAAAEDIVGGIPIVLNAPNKE-KSGIVFVLEKASLEVAKVGK------------ 120

Query: 121 YAFLKFELTIYFPFSNYQLLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAG 180
                           YQLLNSDDH+NFL++NNRNP DYRPDI HQALL I DS + KAG
Sbjct: 121 ---------------TYQLLNSDDHANFLKKNNRNPADYRPDITHQALLMILDSPVNKAG 180

Query: 181 RLKVVYVKTEKGLLIEIKPYVRLPRTQKRF---YVQLLQKLSITAAGKREKLFRVIKNPV 240
           RLK VYV+TEKG+L E+KP+VR+PRT KRF    +QLLQKLSITA   REKL R +KNP+
Sbjct: 181 RLKAVYVRTEKGVLFEVKPHVRIPRTFKRFAGIMLQLLQKLSITAVNSREKLLRCVKNPI 240

Query: 241 TQ-YLPSNSRKMGFSHSSDKLVKVRTYLDAV-EDDVDLVFVVGAMAHGKIETGYTDDLLA 300
            + +LP NS ++GFSHSS+KLV ++ +L  V +DD D VFVVGAMAHGKI+  Y D+ ++
Sbjct: 241 EEHHLPVNSHRIGFSHSSEKLVNMQKHLATVCDDDRDTVFVVGAMAHGKIDCNYIDEFVS 297

Query: 301 ISEYPLSASCCIADICKDLADKWNV 303
           +SEYPLSA+ CI+ IC+ LA  WN+
Sbjct: 301 VSEYPLSAAYCISRICEALATNWNI 297

The following BLAST results are available for this feature:

BLAST of CmoCh14G005180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q06287	9.5e-39	45.50	Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (...	[more]
O35130	1.6e-38	41.42	Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=E...	[more]
Q92979	2.1e-38	39.18	Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EM...	[more]
Q10107	8.0e-38	43.04	Ribosomal RNA small subunit methyltransferase mra1 OS=Schizosaccharomyces pombe ...	[more]
Q9XX15	1.0e-37	40.25	Ribosomal RNA small subunit methyltransferase nep-1 OS=Caenorhabditis elegans OX...	[more]

BLAST of CmoCh14G005180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F634	5.6e-143	89.87	ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita moschata OX=366...	[more]
A0A6J1J0G6	8.1e-142	89.22	ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita maxima OX=3661 ...	[more]
A0A5D3DPQ6	2.7e-129	81.37	Ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3B7L0	2.7e-129	81.37	ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LFI3	7.9e-129	81.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G821550 PE=3 SV=1	[more]

BLAST of CmoCh14G005180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57000.1	4.9e-75	50.77	nucleolar essential protein-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 74..302 e-value: 5.1E-73 score: 246.8
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	PFAM	PF03587	EMG1	coord: 76..295 e-value: 1.2E-57 score: 194.8
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	PANTHER	PTHR12636	NEP1/MRA1	coord: 10..302
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	CDD	cd18088	Nep1-like	coord: 74..293 e-value: 3.11841E-87 score: 257.844
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 12..40
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..51
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 67..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh14G005180.1	CmoCh14G005180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0032040	small-subunit processome
molecular_function	GO:0019843	rRNA binding
molecular_function	GO:0070037	rRNA (pseudouridine) methyltransferase activity