CmoCh14G005110 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh14G005110
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Werner Syndrome-like exonuclease
Location	Cmo_Chr14: 2529015 .. 2529644 (-)
RNA-Seq Expression	CmoCh14G005110
Synteny	CmoCh14G005110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTCACCATCGTTGACCGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGAATCCGATGAGCCGATTCTCACTTTGCTCACCATTTCACCACTCATGGTAGACGATTGGATTTCAGAAACCCTCAACACTCAAACTTCCCCTCTCATCGTCGGCCTCGACATCGAGTGGCGCCCTAACATTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCCGCCGATGCCTGATTGTGCAACTGATTTACGCACCTGAATTCCCTAAATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGGGGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTGGATCTGAGGAATTTGGCTGAGAGCGTTACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGTTGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCAAGCATCATCCAGTTGA

mRNA sequence

ATGGCGCTCACCATCGTTGACCGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGAATCCGATGAGCCGATTCTCACTTTGCTCACCATTTCACCACTCATGGTAGACGATTGGATTTCAGAAACCCTCAACACTCAAACTTCCCCTCTCATCGTCGGCCTCGACATCGAGTGGCGCCCTAACATTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCCGCCGATGCCTGATTGTGCAACTGATTTACGCACCTGAATTCCCTAAATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGGGGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTGGATCTGAGGAATTTGGCTGAGAGCGTTACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGTTGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCAAGCATCATCCAGTTGA

Coding sequence (CDS)

ATGGCGCTCACCATCGTTGACCGTGAAGATCCCTCCGGTTCCCACAATTACTTCGACGTTACTTTCGAATCCGATGAGCCGATTCTCACTTTGCTCACCATTTCACCACTCATGGTAGACGATTGGATTTCAGAAACCCTCAACACTCAAACTTCCCCTCTCATCGTCGGCCTCGACATCGAGTGGCGCCCTAACATTCGCTCCTACGAGAACCCCGTGGCCACGTTACAACTCTGCACCGGCCGCCGATGCCTGATTGTGCAACTGATTTACGCACCTGAATTCCCTAAATCTCTGTATGAGTTTCTGGAAAACGAATCCATCAGTTTTGTAGGAGTCGGAATCGAGGGGGACGCTGAAAAACTGAACTGTGACTACGGATTGAAAGTGAGGAAGACGGTGGATCTGAGGAATTTGGCTGAGAGCGTTACGGGAAGAAGAGAATTGAAGAATGCGGGATTGAAGAGTCTGGCGAAAGAGATTTTGGGGAAAGAGATCCGAAAGCCGAGGGGCGTGACGTTGAGTAGGTGGGATCAAGAATGGCTTACTCATAATCAGATTAAGTATGCTTGTTTTGATGCGTTTGTGTCGTATGAGATTGGAAGGCATTTGCAAGCATCATCCAGTTGA

Protein sequence

MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDIEWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHLQASSS

Homology

[Close]

BLAST of CmoCh14G005110 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-21
Identity = 58/165 (35.15%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 43  ISETLNTQTSPLIVGLDIEWRPNIRSYENP--VATLQLCTGRRCLIVQLIYAPEFPKSLY 102
           + +T   ++    VGLDIEWRP+ R    P  VAT+Q+C       V  I+    P+SL 
Sbjct: 117 VLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQ 176

Query: 103 EFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKE 162
             +E+ ++  VG+GI+GD+ KL  DYG+ ++   DL +LA    G    K  GL SL + 
Sbjct: 177 HLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTET 236

Query: 163 ILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHLQ 206
           ++ KE+ KP  + L  W+   L+  Q++YA  DA+ S+ + + L+
Sbjct: 237 LVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]

BLAST of CmoCh14G005110 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 8.3e-18
Identity = 63/191 (32.98%), Postives = 95/191 (49.74%), Query Frame = 0


Query: 22  FESDEPILTLLTISPLMVD----DWISETLNTQTSP-LIVGLDIEWRPNI-RSYENPVAT 81
           FE D P L          D     ++SE ++   S   +VG D+EW P   R     VA 
Sbjct: 40  FEDDLPFLEFTGSIVYSYDASDCSFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVAL 99

Query: 82  LQLCTGR-RCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAEKLNCDYGLKVRKTV 141
           +QLC    +C +  +     FP+ L   LEN+++   GVGIEGD  KL  D+ +K++  V
Sbjct: 100 IQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFV 159

Query: 142 DLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDA 201
           +L ++A       E     L SL K +LGK++ K + +  S W +  LT +Q  YA  DA
Sbjct: 160 ELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDA 219

Query: 202 FVSYEIGRHLQ 206
           +  + I R+L+
Sbjct: 220 YAGFIIYRNLE 228

[Close]

BLAST of CmoCh14G005110 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 87.0 bits (214), Expect = 2.7e-16
Identity = 55/155 (35.48%), Postives = 80/155 (51.61%), Query Frame = 0


Query: 55  IVGLDIEWRPNIR-SYENPVATLQLCTGR-RCLIVQLIYAPEFPKSLYEFLENESISFVG 114
           +VG D+EW P  +    + VA +QLC    +C +  +     FP+ L   LEN+SI   G
Sbjct: 72  VVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAG 131

Query: 115 VGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNA---GLKSLAKEILGKEIRKP 174
           VGIEGD  KL  D+ +K+   V+L ++A       +LK A    L  L K +LGK++ K 
Sbjct: 132 VGIEGDQWKLLRDFDVKLESFVELTDVA-----NEKLKCAETWSLNGLVKHVLGKQLLKD 191

Query: 175 RGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
           + +  S W    LT +Q  YA  DA+    I + L
Sbjct: 192 KSIRCSNWSNFPLTEDQKLYAATDAYAGLIIYQKL 221

[Close]

BLAST of CmoCh14G005110 vs. ExPASy Swiss-Prot
Match: Q9NVH0 (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE=1 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-15
Identity = 60/180 (33.33%), Postives = 91/180 (50.56%), Query Frame = 0


Query: 31  LLTISPLMVDDWISETLNTQTSPL-IVGLDIEWRPNIRSYENPVATLQLCT-GRRCLIVQ 90
           ++T+S     D I   L ++     ++G+D EW  N+    +P++ LQ+ +    C++V+
Sbjct: 79  VVTVSQEAEWDQIEPLLRSELEDFPVLGIDCEW-VNLEGKASPLSLLQMASPSGLCVLVR 138

Query: 91  L----IYAPEFPKSLYEFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVT 150
           L          P++L + L + +I  VGVG   DA KL  DYGL VR  +DLR LA    
Sbjct: 139 LPKLICGGKTLPRTLLDILADGTILKVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQR 198

Query: 151 GRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
                    LKSLA+ +L   + K   +  S WD E LT +Q+ YA  DA +S  +  HL
Sbjct: 199 NNLLCNGLSLKSLAETVLNFPLDKSLLLRCSNWDAETLTEDQVIYAARDAQISVALFLHL 257

[Close]

BLAST of CmoCh14G005110 vs. ExPASy Swiss-Prot
Match: Q8VEG4 (Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus OX=10090 GN=Exd2 PE=1 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 58/155 (37.42%), Postives = 82/155 (52.90%), Query Frame = 0


Query: 55  IVGLDIEWRPNIRSYENPVATLQLCT-GRRCLIV---QLIYAPE-FPKSLYEFLENESIS 114
           ++G+D EW  N+    +P++ LQ+ +    C +V   +LIY     P++L + L + +I 
Sbjct: 133 VLGIDCEW-VNLEGKASPLSLLQMASPSGFCALVRLPRLIYGGRTLPRTLLDILADGAIL 192

Query: 115 FVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKP 174
            VGVG   DA KL  DYGL VR  +DLR LA             LKSLA+ IL   + K 
Sbjct: 193 KVGVGCSEDANKLLQDYGLIVRGCLDLRYLAMKQGNNILCNGLSLKSLAETILNFPLDKS 252

Query: 175 RGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
             +  S WD E LT +Q+ YA  DA +S  +  HL
Sbjct: 253 LLLRCSNWDAENLTEDQVTYAARDAQISVALFLHL 286

[Close]

BLAST of CmoCh14G005110 vs. ExPASy TrEMBL
Match: A0A6J1F975 (Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111441964 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 9.0e-116
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE
Sbjct: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE
Sbjct: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           WLTHNQIKYACFDAFVSYEIGRHLQASSS
Sbjct: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 209

[Close]

BLAST of CmoCh14G005110 vs. ExPASy TrEMBL
Match: A0A6J1J8Y2 (Werner Syndrome-like exonuclease OS=Cucurbita maxima OX=3661 GN=LOC111482356 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 9.9e-107
Identity = 191/209 (91.39%), Postives = 200/209 (95.69%), Query Frame = 0


Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           MALTIV+REDPSGSHNYFDVTF+SD+PILTLLT SP MV+DWI+ETLNTQTSPL+VGLDI
Sbjct: 1   MALTIVEREDPSGSHNYFDVTFDSDQPILTLLTTSPFMVEDWIAETLNTQTSPLVVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSYENPVATLQLCTG RCLI+QLIY PEFP SLYEFLENESISFVGVGIE DAE
Sbjct: 61  EWRPNSRSYENPVATLQLCTGSRCLIMQLIYTPEFPISLYEFLENESISFVGVGIEDDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKVRKTVDLRNLAE VTGRRELKNAGLKSLAKEILGKEIRKPRGVT+SRWDQE
Sbjct: 121 KLNCDYGLKVRKTVDLRNLAERVTGRRELKNAGLKSLAKEILGKEIRKPRGVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           WLTHNQIKYACFDAFVSYEIGRHL +SSS
Sbjct: 181 WLTHNQIKYACFDAFVSYEIGRHLLSSSS 209

[Close]

BLAST of CmoCh14G005110 vs. ExPASy TrEMBL
Match: A0A5A7UI20 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006600 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 5.0e-90
Identity = 161/209 (77.03%), Postives = 183/209 (87.56%), Query Frame = 0


Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           M++TIVD E PS SHNYFD+TF++DEPILTLLT SP MVDDWIS+TL  QT PL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           WLT NQ+KYAC DAF S+EIGR LQ+ SS
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]

BLAST of CmoCh14G005110 vs. ExPASy TrEMBL
Match: E5GBP0 (3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 5.0e-90
Identity = 161/209 (77.03%), Postives = 183/209 (87.56%), Query Frame = 0


Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           M++TIVD E PS SHNYFD+TF++DEPILTLLT SP MVDDWIS+TL  QT PL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           WLT NQ+KYAC DAF S+EIGR LQ+ SS
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]

BLAST of CmoCh14G005110 vs. ExPASy TrEMBL
Match: A0A1S3B738 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 5.0e-90
Identity = 161/209 (77.03%), Postives = 183/209 (87.56%), Query Frame = 0


Query: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTSPLIVGLDI 60
           M++TIVD E PS SHNYFD+TF++DEPILTLLT SP MVDDWIS+TL  QT PL+VGLDI
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120
           EWRPN RSY+NPVATLQLC GRRCLI+QL++ PE PKSL+EFLENES +FVGVGI+ DAE
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180
           KLNCDYGLKV K +DLR+LAESVTGR ELKNAGLK L KE+LGKEI KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLTHNQIKYACFDAFVSYEIGRHLQASSS 210
           WLT NQ+KYAC DAF S+EIGR LQ+ SS
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQSESS 209

[Close]

BLAST of CmoCh14G005110 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 104.8 bits (260), Expect = 8.8e-23
Identity = 58/164 (35.37%), Postives = 91/164 (55.49%), Query Frame = 0


Query: 43  ISETLNTQTSPLIVGLDIEWRPNIRSYENP--VATLQLCTGRRCLIVQLIYAPEFPKSLY 102
           + +T   ++    VGLDIEWRP+ R    P  VAT+Q+C       V  I+    P+SL 
Sbjct: 117 VLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQ 176

Query: 103 EFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKE 162
             +E+ ++  VG+GI+GD+ KL  DYG+ ++   DL +LA    G    K  GL SL + 
Sbjct: 177 HLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTET 236

Query: 163 ILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHL 205
           ++ KE+ KP  + L  W+   L+  Q++YA  DA+ S+ + + L
Sbjct: 237 LVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

[Close]

BLAST of CmoCh14G005110 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 104.4 bits (259), Expect = 1.2e-22
Identity = 58/165 (35.15%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 43  ISETLNTQTSPLIVGLDIEWRPNIRSYENP--VATLQLCTGRRCLIVQLIYAPEFPKSLY 102
           + +T   ++    VGLDIEWRP+ R    P  VAT+Q+C       V  I+    P+SL 
Sbjct: 117 VLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQ 176

Query: 103 EFLENESISFVGVGIEGDAEKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKE 162
             +E+ ++  VG+GI+GD+ KL  DYG+ ++   DL +LA    G    K  GL SL + 
Sbjct: 177 HLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTET 236

Query: 163 ILGKEIRKPRGVTLSRWDQEWLTHNQIKYACFDAFVSYEIGRHLQ 206
           ++ KE+ KP  + L  W+   L+  Q++YA  DA+ S+ + + L+
Sbjct: 237 LVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]

BLAST of CmoCh14G005110 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.2e-21
Identity = 55/151 (36.42%), Postives = 86/151 (56.95%), Query Frame = 0


Query: 53  PLIVGLDIEWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVG 112
           PL+VGLD++W P     + P   LQLC G RCLI+QL +    P+ L  FLE+E+I+FVG
Sbjct: 55  PLVVGLDVQWTPG--GSDPPPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVG 114

Query: 113 VGIEGDAEKL-NCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILG-KEIRKPR 172
           V    D  KL    + L++ + +D+R+   +      L N+  + + +E LG K +RK +
Sbjct: 115 VWNSQDQGKLERFRHQLEIWRLLDIRHYLPT-----RLLNSSFEKIVEECLGYKGVRKDK 174

Query: 173 GVTLSRWDQEWLTHNQIKYACFDAFVSYEIG 202
            + +S W    L+H+QI  A  D +V  ++G
Sbjct: 175 EICMSNWGARSLSHDQIVQASDDVYVCCKLG 198

[Close]

BLAST of CmoCh14G005110 vs. TAIR 10
Match: AT3G12470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 2.4e-20
Identity = 62/193 (32.12%), Postives = 99/193 (51.30%), Query Frame = 0


Query: 14  SHNYFDVTFESDEPILTLLTISPLMVDDWI----SETLNTQTSPLIVGLDIEWRPNIRSY 73
           +H    V F  D+ I+T +T +  ++  WI    S   N    PL+VG+ ++WRP+    
Sbjct: 13  THQKHFVDFFGDDLIVT-VTPTASVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSSDP 72

Query: 74  ENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAEKL-NCDYGL 133
           + P  TLQLC G RCLI+QL Y    PK L  FL +   +FVGV    D +KL  C + +
Sbjct: 73  DPPPKTLQLCVGSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRV 132

Query: 134 KVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGK-EIRKPRGVTLSRWDQEWLTHNQI 193
           ++ K +D+R       G R +     + + KE LG+  +R    + +S W    L H Q+
Sbjct: 133 EIGKLLDIRMFVRDSRGAR-MCFCSFEQIVKERLGRVGVRLDPAICMSDWGVYNLNHYQV 192

Query: 194 KYACFDAFVSYEI 201
             A  +++V +++
Sbjct: 193 LQASIESYVCFKL 203

[Close]

BLAST of CmoCh14G005110 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 9.1e-20
Identity = 66/211 (31.28%), Postives = 106/211 (50.24%), Query Frame = 0


Query: 14  SHNYFDVTFESDEPILTLLTISPLMVDDWISETL---NTQTSPLIVGLDIEW-------- 73
           +H  + V F  DE I+T +T    ++  WI   L      + PL+VG+ ++W        
Sbjct: 14  THLEYSVDFFGDEFIVT-VTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYSD 73

Query: 74  -RPN--------IRSY-ENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVG 133
            RPN        IR Y +NP   LQLC G RCLI+QL Y  + P +L  FL +   +FVG
Sbjct: 74  PRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFVG 133

Query: 134 VGIEGDAEKL-NCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKE-IRKPR 193
           V    DA KL  C + L++ + +D+R       G R ++ +  + + +E +G + +    
Sbjct: 134 VWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWG-RSMRRSSFEEIVEECMGYQGVMLDP 193

Query: 194 GVTLSRWDQEWLTHNQIKYACFDAFVSYEIG 202
            +++S W    L  +QI  A  DA+V +++G
Sbjct: 194 EISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

The following BLAST results are available for this feature:

BLAST of CmoCh14G005110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	1.6e-21	35.15	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	8.3e-18	32.98	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	2.7e-16	35.48	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
Q9NVH0	1.0e-15	33.33	Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens OX=9606 GN=EXD2 PE...	[more]
Q8VEG4	1.3e-15	37.42	Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus OX=10090 GN=Exd2 P...	[more]

BLAST of CmoCh14G005110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F975	9.0e-116	100.00	Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111441964 P...	[more]
A0A6J1J8Y2	9.9e-107	91.39	Werner Syndrome-like exonuclease OS=Cucurbita maxima OX=3661 GN=LOC111482356 PE=...	[more]
A0A5A7UI20	5.0e-90	77.03	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
E5GBP0	5.0e-90	77.03	3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A1S3B738	5.0e-90	77.03	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV...	[more]

BLAST of CmoCh14G005110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13870.1	8.8e-23	35.37	Werner syndrome-like exonuclease	[more]
AT4G13870.2	1.2e-22	35.15	Werner syndrome-like exonuclease	[more]
AT2G36110.1	8.2e-21	36.42	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12470.1	2.4e-20	32.12	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12410.1	9.1e-20	31.28	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 36..209 e-value: 7.1E-8 score: 42.2
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 40..205 e-value: 8.0E-19 score: 68.1
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 20..206 e-value: 1.1E-59 score: 203.3
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 12..207
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 12..207
None	No IPR available	CDD	cd06141	WRN_exo	coord: 33..206 e-value: 3.50798E-66 score: 199.342
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 46..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh14G005110.1	CmoCh14G005110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding