CmoCh13G010750 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G010750
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein ASPARTIC PROTEASE IN GUARD CELL 1-like
LocationCmo_Chr13: 8912979 .. 8914463 (-)
RNA-Seq ExpressionCmoCh13G010750
SyntenyCmoCh13G010750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACTGCAAATCTGCTACCTCTGTTCTCCTTCGCCAGCTTCTTTTTCTCTCTCTTGCCCTTCACTCTGTGTCGCACTTCGCCGGAGCTTTCTCACTCTTCCTCCTCCTCCGCTTTTCTCGATGTTTCGGCTTCACTGAAACAAGCCAACCAGGTCCTCAAATTCGACCCCAAGGCTTTGCTATCCTTCCAGCAGCAGGATGAGGTTGTTCCGGTGAACTCTTCTTCCTCCTTTTCTCTGCAACTGCACTCTCGCGACGCTCTTCAAAATGCCGGACATCAGGACTACAAGAGCCTGGTCCTTTCCCGGCTGGACCGGGACTCTTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTTCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGCAAACGGAGATTCTGCCAGAGGATCTGAGCACTCCGATTGTCTCTGGCACCAGTCAAGGAAGCGGCGAGTATTTCTCCCGCATCGGCGTCGGACGACCGGCTAAGCCGTTCTATATGGTTCTTGACACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGACTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCGTCTTCTTTTGCTTCACTTCCCTGCGAATCTCCGCAGTGTCAAGCTCTAGAAACGTCTGGCTGTCGTGCTGCGAAGTGTCTTTACCAGGTCGCGTACGGCGATGGCTCCTTCACTGTCGGTGAGTTCGTTACTGAATCTCTGTCGTTTGGCAACTCCGGGACGATTAACAATGTCGCTCTTGGCTGCGGCCACGACAACGAAGGATTATTCGTCGGATCCGCCGGATTGCTCGGACTCGGCGGCGGATCACTATCCCTCACTTCTCAGATGAAAGCGTCGTCATTCTCATACTGTCTTGTGGACCGCGACTCTAGTTCGTCTTCAGCTCTCGAGTTCAATTCCGCTGCACCGAGTGACTCAGTGAATGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGGACTGGTCGGATTGAGCGTCGGCGGCCAGTTACTGTCAATTCCTCCTTCTCTGTTTCAAATGGACGATGCCGGCTACGGCGGTATCATCGTCGATTCAGGAACCGCCATAACTCGACTGCAGACTCAAGCCTACAACACCCTTCGCGACGCCTTCGTGAGTCGCACACCATACCTGAGGAGGACAAACGGCTTCGCATTATTCGACACATGTTACGATCTATCATCCCAGTCAAGAGTCACCATTCCAACGCTGTCATTTCAATTCGCCGGCGGGAAGTCTCTGCAGCTGCCGCCAAAGAACTACCTCATCCCAGTGGACTCCGTCGGAACTTTCTGTTTGGCGTTCGCCCCAACGACGTCGTCTTTGTCCATCATAGGGAACGTCCAGCAGCAAGGGACACGTGTCCACTTCGATTTGGCAAATTCACTCGTAGGGTTTTCACCCAACAAATGTTAG

mRNA sequence

ATGGCGACTGCAAATCTGCTACCTCTGTTCTCCTTCGCCAGCTTCTTTTTCTCTCTCTTGCCCTTCACTCTGTGTCGCACTTCGCCGGAGCTTTCTCACTCTTCCTCCTCCTCCGCTTTTCTCGATGTTTCGGCTTCACTGAAACAAGCCAACCAGGTCCTCAAATTCGACCCCAAGGCTTTGCTATCCTTCCAGCAGCAGGATGAGGTTGTTCCGGTGAACTCTTCTTCCTCCTTTTCTCTGCAACTGCACTCTCGCGACGCTCTTCAAAATGCCGGACATCAGGACTACAAGAGCCTGGTCCTTTCCCGGCTGGACCGGGACTCTTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTTCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGCAAACGGAGATTCTGCCAGAGGATCTGAGCACTCCGATTGTCTCTGGCACCAGTCAAGGAAGCGGCGAGTATTTCTCCCGCATCGGCGTCGGACGACCGGCTAAGCCGTTCTATATGGTTCTTGACACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGACTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCGTCTTCTTTTGCTTCACTTCCCTGCGAATCTCCGCAGTGTCAAGCTCTAGAAACGTCTGGCTGTCGTGCTGCGAAGTGTCTTTACCAGGTCGCGTACGGCGATGGCTCCTTCACTGTCGGTGAGTTCGTTACTGAATCTCTGTCGTTTGGCAACTCCGGGACGATTAACAATGTCGCTCTTGGCTGCGGCCACGACAACGAAGGATTATTCGTCGGATCCGCCGGATTGCTCGGACTCGGCGGCGGATCACTATCCCTCACTTCTCAGATGAAAGCGTCGTCATTCTCATACTGTCTTGTGGACCGCGACTCTAGTTCGTCTTCAGCTCTCGAGTTCAATTCCGCTGCACCGAGTGACTCAGTGAATGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGGACTGGTCGGATTGAGCGTCGGCGGCCAGTTACTGTCAATTCCTCCTTCTCTGTTTCAAATGGACGATGCCGGCTACGGCGGTATCATCGTCGATTCAGGAACCGCCATAACTCGACTGCAGACTCAAGCCTACAACACCCTTCGCGACGCCTTCGTGAGTCGCACACCATACCTGAGGAGGACAAACGGCTTCGCATTATTCGACACATGTTACGATCTATCATCCCAGTCAAGAGTCACCATTCCAACGCTGTCATTTCAATTCGCCGGCGGGAAGTCTCTGCAGCTGCCGCCAAAGAACTACCTCATCCCAGTGGACTCCGTCGGAACTTTCTGTTTGGCGTTCGCCCCAACGACGTCGTCTTTGTCCATCATAGGGAACGTCCAGCAGCAAGGGACACGTGTCCACTTCGATTTGGCAAATTCACTCGTAGGGTTTTCACCCAACAAATGTTAG

Coding sequence (CDS)

ATGGCGACTGCAAATCTGCTACCTCTGTTCTCCTTCGCCAGCTTCTTTTTCTCTCTCTTGCCCTTCACTCTGTGTCGCACTTCGCCGGAGCTTTCTCACTCTTCCTCCTCCTCCGCTTTTCTCGATGTTTCGGCTTCACTGAAACAAGCCAACCAGGTCCTCAAATTCGACCCCAAGGCTTTGCTATCCTTCCAGCAGCAGGATGAGGTTGTTCCGGTGAACTCTTCTTCCTCCTTTTCTCTGCAACTGCACTCTCGCGACGCTCTTCAAAATGCCGGACATCAGGACTACAAGAGCCTGGTCCTTTCCCGGCTGGACCGGGACTCTTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTTCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGCAAACGGAGATTCTGCCAGAGGATCTGAGCACTCCGATTGTCTCTGGCACCAGTCAAGGAAGCGGCGAGTATTTCTCCCGCATCGGCGTCGGACGACCGGCTAAGCCGTTCTATATGGTTCTTGACACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGACTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCGTCTTCTTTTGCTTCACTTCCCTGCGAATCTCCGCAGTGTCAAGCTCTAGAAACGTCTGGCTGTCGTGCTGCGAAGTGTCTTTACCAGGTCGCGTACGGCGATGGCTCCTTCACTGTCGGTGAGTTCGTTACTGAATCTCTGTCGTTTGGCAACTCCGGGACGATTAACAATGTCGCTCTTGGCTGCGGCCACGACAACGAAGGATTATTCGTCGGATCCGCCGGATTGCTCGGACTCGGCGGCGGATCACTATCCCTCACTTCTCAGATGAAAGCGTCGTCATTCTCATACTGTCTTGTGGACCGCGACTCTAGTTCGTCTTCAGCTCTCGAGTTCAATTCCGCTGCACCGAGTGACTCAGTGAATGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGGACTGGTCGGATTGAGCGTCGGCGGCCAGTTACTGTCAATTCCTCCTTCTCTGTTTCAAATGGACGATGCCGGCTACGGCGGTATCATCGTCGATTCAGGAACCGCCATAACTCGACTGCAGACTCAAGCCTACAACACCCTTCGCGACGCCTTCGTGAGTCGCACACCATACCTGAGGAGGACAAACGGCTTCGCATTATTCGACACATGTTACGATCTATCATCCCAGTCAAGAGTCACCATTCCAACGCTGTCATTTCAATTCGCCGGCGGGAAGTCTCTGCAGCTGCCGCCAAAGAACTACCTCATCCCAGTGGACTCCGTCGGAACTTTCTGTTTGGCGTTCGCCCCAACGACGTCGTCTTTGTCCATCATAGGGAACGTCCAGCAGCAAGGGACACGTGTCCACTTCGATTTGGCAAATTCACTCGTAGGGTTTTCACCCAACAAATGTTAG

Protein sequence

MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLVGFSPNKC
Homology
BLAST of CmoCh13G010750 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 8.5e-162
Identity = 295/495 (59.60%), Postives = 373/495 (75.35%), Query Frame = 0

Query: 16  FFSLL---PFTLCRTSPELSHSSSSSA----FLDVSASLKQANQVLKFDP--KALLSFQQ 75
           F SLL     +L  T+ + S  S S+      LDV +SL+Q   +L  DP   +L + + 
Sbjct: 6   FLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKP 65

Query: 76  QDEVVPV--NSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLS 135
           +    PV  NSSS  SL+LHSRD    + H+DYKSL LSRL+RDSSRV  +  ++ F++ 
Sbjct: 66  ESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVE 125

Query: 136 DLKRSDLQPL---QTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDI 195
            + RSDL+P+    T    EDL+TP+VSG SQGSGEYFSRIGVG PAK  Y+VLDTGSD+
Sbjct: 126 GVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDV 185

Query: 196 NWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGS 255
           NW+QC+PC DCYQQ+DP+F+P +SS++ SL C +PQC  LETS CR+ KCLYQV+YGDGS
Sbjct: 186 NWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGS 245

Query: 256 FTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFS 315
           FTVGE  T++++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+SFS
Sbjct: 246 FTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFS 305

Query: 316 YCLVDRDSSSSSALEFNSA-APSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 375
           YCLVDRDS  SS+L+FNS         APLLR+ ++DTFYYVGL G SVGG+ + +P ++
Sbjct: 306 YCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAI 365

Query: 376 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRR-TNGFALFDTCYDLSSQ 435
           F +D +G GG+I+D GTA+TRLQTQAYN+LRDAF+  T  L++ ++  +LFDTCYD SS 
Sbjct: 366 FDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSL 425

Query: 436 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 495
           S V +PT++F F GGKSL LP KNYLIPVD  GTFC AFAPT+SSLSIIGNVQQQGTR+ 
Sbjct: 426 STVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRIT 485

BLAST of CmoCh13G010750 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 8.9e-111
Identity = 245/497 (49.30%), Postives = 308/497 (61.97%), Query Frame = 0

Query: 9   LFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA---LLSFQ 68
           LFS   FF SL         P  S   S       S SL  A+ V  F P +    L   
Sbjct: 9   LFSLCFFFLSL---------PSFSSLPSFQTLFPNSHSLPCASPV-SFQPDSDSESLLES 68

Query: 69  QQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSD 128
           + +      SSSS +L L   DAL +    D   L  SRL RDS RVKS+        + 
Sbjct: 69  EFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSI-------ATL 128

Query: 129 LKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQ 188
             +   + +     P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQ
Sbjct: 129 AAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQ 188

Query: 189 CQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGC--RAAKCLYQVAYGDGSFT 248
           C PC  CY Q+DPIFDPR S ++A++PC SP C+ L+++GC  R   CLYQV+YGDGSFT
Sbjct: 189 CAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFT 248

Query: 249 VGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL---TSQMKASSF 308
           VG+F TE+L+F     +  VALGCGHDNEGLFVG+AGLLGLG G LS    T       F
Sbjct: 249 VGDFSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKF 308

Query: 309 SYCLVDRDSSSS-SALEFNSAAPSDSVN-APLLRSGRVDTFYYVGLVGLSVGG-QLLSIP 368
           SYCLVDR +SS  S++ F +AA S      PLL + ++DTFYYVGL+G+SVGG ++  + 
Sbjct: 309 SYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVT 368

Query: 369 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLS 428
            SLF++D  G GG+I+DSGT++TRL   AY  +RDAF      L+R   F+LFDTC+DLS
Sbjct: 369 ASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLS 428

Query: 429 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 488
           + + V +PT+   F G   + LP  NYLIPVD+ G FC AFA T   LSIIGN+QQQG R
Sbjct: 429 NMNEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFR 484

Query: 489 VHFDLANSLVGFSPNKC 495
           V +DLA+S VGF+P  C
Sbjct: 489 VVYDLASSRVGFAPGGC 484

BLAST of CmoCh13G010750 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.6e-104
Identity = 214/497 (43.06%), Postives = 295/497 (59.36%), Query Frame = 0

Query: 6   LLPLFSFASFFFSLLPFTLCRTS----PELSHSSSSSAFLDVSASLKQANQVLKFDPKAL 65
           LLPLF    FFF  L   L  +S    P+          L V+A+L   N     D    
Sbjct: 2   LLPLF----FFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSD---- 61

Query: 66  LSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEF 125
                        SSS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+  
Sbjct: 62  ------------ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISG 121

Query: 126 SLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDI 185
            +  +  SD     +     D  + IVSG  QGSGEYF RIGVG P +  YMV+D+GSD+
Sbjct: 122 KV--IPSSD-----SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDM 181

Query: 186 NWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGS 245
            W+QCQPC  CY+Q+DP+FDP  S S+  + C S  C  +E SGC +  C Y+V YGDGS
Sbjct: 182 VWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGS 241

Query: 246 FTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK---AS 305
           +T G    E+L+F  +  + NVA+GCGH N G+F+G+AGLLG+GGGS+S   Q+      
Sbjct: 242 YTKGTLALETLTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGG 301

Query: 306 SFSYCLVDRDSSSSSALEF-NSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIP 365
           +F YCLV R + S+ +L F   A P  +   PL+R+ R  +FYYVGL GL VGG  + +P
Sbjct: 302 AFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLP 361

Query: 366 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLS 425
             +F + + G GG+++D+GTA+TRL T AY   RD F S+T  L R +G ++FDTCYDLS
Sbjct: 362 DGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLS 421

Query: 426 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 485
               V +PT+SF F  G  L LP +N+L+PVD  GT+C AFA + + LSIIGN+QQ+G +
Sbjct: 422 GFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQ 470

Query: 486 VHFDLANSLVGFSPNKC 495
           V FD AN  VGF PN C
Sbjct: 482 VSFDGANGFVGFGPNVC 470

BLAST of CmoCh13G010750 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.9e-73
Identity = 155/347 (44.67%), Postives = 208/347 (59.94%), Query Frame = 0

Query: 154 GSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPC 213
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ Q+ PIF+P+ SSSF++LPC
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 214 ESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEG 273
            S  CQAL +  C    C Y   YGDGS T G   TE+L+FG S +I N+  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 274 LFVGS-AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSSSSSALEFNSAAPS---DSVNAP 333
              G+ AGL+G+G G LSL SQ+  + FSYC+    SS+ S L   S A S    S N  
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 334 LLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMD-DAGYGGIIVDSGTAITRLQTQAYN 393
           L++S ++ TFYY+ L GLSVG   L I PS F ++ + G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 394 TLRDAFVSRTPYLRRTNGFALFDTCYDL-SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIP 453
           ++R  F+S+          + FD C+   S  S + IPT    F GG  L+LP +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 454 VDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLVGFSPNKC 495
             S G  CLA   ++  +SI GN+QQQ   V +D  NS+V F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of CmoCh13G010750 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.3e-72
Identity = 169/442 (38.24%), Postives = 232/442 (52.49%), Query Frame = 0

Query: 70  VVPVNSSSSFSLQLHSRDALQNAGHQDYK-----------SLVLSRLDRDSSRVKSLNDR 129
           V P +S+S  +L  H +   Q     D +            L+   + R   R++S+N  
Sbjct: 19  VAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAM 78

Query: 130 LEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTG 189
           L+ S                    + TP+ +    G GEY   + +G P   F  ++DTG
Sbjct: 79  LQSS------------------SGIETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTG 138

Query: 190 SDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYG 249
           SD+ W QC+PCT C+ Q  PIF+P+ SSSF++LPCES  CQ L +  C   +C Y   YG
Sbjct: 139 SDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYG 198

Query: 250 DGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGS-AGLLGLGGGSLSLTSQMKA 309
           DGS T G   TE+ +F  S ++ N+A GCG DN+G   G+ AGL+G+G G LSL SQ+  
Sbjct: 199 DGSTTQGYMATETFTFETS-SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV 258

Query: 310 SSFSYCLVDRDSSSSSALEFNSAA---PSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLL 369
             FSYC+    SSS S L   SAA   P  S +  L+ S    T+YY+ L G++VGG  L
Sbjct: 259 GQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNL 318

Query: 370 SIPPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCY 429
            IP S FQ+ D G GG+I+DSGT +T L   AYN +  AF  +          +   TC+
Sbjct: 319 GIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCF 378

Query: 430 DLSSQ-SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTS-SLSIIGNVQ 489
              S  S V +P +S QF GG  L L  +N LI   + G  CLA   ++   +SI GN+Q
Sbjct: 379 QQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQ 435

Query: 490 QQGTRVHFDLANSLVGFSPNKC 495
           QQ T+V +DL N  V F P +C
Sbjct: 439 QQETQVLYDLQNLAVSFVPTQC 435

BLAST of CmoCh13G010750 vs. ExPASy TrEMBL
Match: A0A6J1E815 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431581 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.3e-278
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA 60
           MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA
Sbjct: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA 60

Query: 61  LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE 120
           LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE
Sbjct: 61  LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE 120

Query: 121 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 180
           FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD
Sbjct: 121 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 180

Query: 181 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 240
           INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG
Sbjct: 181 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 240

Query: 241 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300
           SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF
Sbjct: 241 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300

Query: 301 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 360
           SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL
Sbjct: 301 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 360

Query: 361 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 420
           FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS
Sbjct: 361 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 420

Query: 421 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 480
           RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF
Sbjct: 421 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 480

Query: 481 DLANSLVGFSPNKC 495
           DLANSLVGFSPNKC
Sbjct: 481 DLANSLVGFSPNKC 494

BLAST of CmoCh13G010750 vs. ExPASy TrEMBL
Match: A0A6J1KJJ8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495193 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 2.6e-270
Identity = 483/495 (97.58%), Postives = 488/495 (98.59%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH-SSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANL PLFSFA FFFSLLPFTLCRTSPELSH SSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQ+EVVP NSSSSFSLQLHSRDALQNAGH+DYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVG+PAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASL CESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSD+VNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNG ALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLVGFSPNKC 495
           FDLANSL+GFSPNKC
Sbjct: 481 FDLANSLLGFSPNKC 495

BLAST of CmoCh13G010750 vs. ExPASy TrEMBL
Match: A0A6J1C6K9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN=LOC111008878 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 9.2e-244
Identity = 436/490 (88.98%), Postives = 464/490 (94.69%), Query Frame = 0

Query: 6   LLPLFSFASFFFSL-LPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKALLSF 65
           +LP+F+   F FSL LPFT CR+SPELS   SSSA LDVSASLKQA+QVLKFDPK L+SF
Sbjct: 2   VLPVFTI--FIFSLFLPFTSCRSSPELSR--SSSALLDVSASLKQADQVLKFDPKGLMSF 61

Query: 66  QQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLS 125
           QQQ+++VP N SSSFSLQLH RDAL+NAGH+DYKSLVLSRLDRDSSRVKSLNDRL+F+LS
Sbjct: 62  QQQEQLVPAN-SSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALS 121

Query: 126 DLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWL 185
           DLKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDTGSDINWL
Sbjct: 122 DLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWL 181

Query: 186 QCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFTV 245
           QCQPCTDCYQQ+DPIFDPRTSSSFASLPCES QCQALE SGCRAAKCLYQV+YGDGSFTV
Sbjct: 182 QCQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTV 241

Query: 246 GEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCL 305
           GEFVTE+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCL
Sbjct: 242 GEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCL 301

Query: 306 VDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMD 365
           VDRDS SSS+L+FNS APSDSV APLLRSGRVDTFYYVGL GLSVGGQLLSIPPSLFQMD
Sbjct: 302 VDRDSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMD 361

Query: 366 DAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTI 425
           D+GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNGFALFDTCYDLSSQSRVTI
Sbjct: 362 DSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTI 421

Query: 426 PTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLAN 485
           PT+SF F+GGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLAN
Sbjct: 422 PTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLAN 481

Query: 486 SLVGFSPNKC 495
           SLVGFSPNKC
Sbjct: 482 SLVGFSPNKC 486

BLAST of CmoCh13G010750 vs. ExPASy TrEMBL
Match: A0A5D3BI72 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001860 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.9e-242
Identity = 431/494 (87.25%), Postives = 464/494 (93.93%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA 60
           MAT+NLL LF    FFFSL PF L R+S  LS  SS+S  LDVSASL+QANQVLKFDP A
Sbjct: 1   MATSNLLTLF---LFFFSLFPFALSRSSSHLSPHSSAS--LDVSASLQQANQVLKFDPTA 60

Query: 61  LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE 120
            +SFQQQ  +VP NSS SFSLQLH RD+L NAGH+DYKSLVLSRL RDSSRVKS+ DRLE
Sbjct: 61  SISFQQQVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLE 120

Query: 121 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 180
           F+LS+LKRSDL+PL+TEILPEDLSTPIVSGTSQGSGEYFSR+GVG+PAKPFYMVLDTGSD
Sbjct: 121 FALSELKRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSD 180

Query: 181 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 240
           INWLQCQPCTDCYQQTDPIFDPR+SSSFASLPCES QCQALETSGCRA+KCLYQV+YGDG
Sbjct: 181 INWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDG 240

Query: 241 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300
           SFTVGEFVTE+L+FGNSG I NVA+GCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF
Sbjct: 241 SFTVGEFVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300

Query: 301 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 360
           SYCLVDRDSSSSS LEFNSAAP+DSVNAPLL+SGRVDTFYYVGL G+SVGGQLLSIPP+L
Sbjct: 301 SYCLVDRDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNL 360

Query: 361 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 420
           FQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNGFALFDTCYDLSSQS
Sbjct: 361 FQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQS 420

Query: 421 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 480
           RVTIPT+SF+FAGGKSLQLPPKNYLIPVDSVGTFC AFAPTTSSLSIIGNVQQQGTRVH+
Sbjct: 421 RVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHY 480

Query: 481 DLANSLVGFSPNKC 495
           DLANS+VGFSP+KC
Sbjct: 481 DLANSVVGFSPHKC 489

BLAST of CmoCh13G010750 vs. ExPASy TrEMBL
Match: A0A1S3C5F6 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103496838 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.9e-242
Identity = 431/494 (87.25%), Postives = 464/494 (93.93%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA 60
           MAT+NLL LF    FFFSL PF L R+S  LS  SS+S  LDVSASL+QANQVLKFDP A
Sbjct: 48  MATSNLLTLF---LFFFSLFPFALSRSSSHLSPHSSAS--LDVSASLQQANQVLKFDPTA 107

Query: 61  LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE 120
            +SFQQQ  +VP NSS SFSLQLH RD+L NAGH+DYKSLVLSRL RDSSRVKS+ DRLE
Sbjct: 108 SISFQQQVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLE 167

Query: 121 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 180
           F+LS+LKRSDL+PL+TEILPEDLSTPIVSGTSQGSGEYFSR+GVG+PAKPFYMVLDTGSD
Sbjct: 168 FALSELKRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSD 227

Query: 181 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 240
           INWLQCQPCTDCYQQTDPIFDPR+SSSFASLPCES QCQALETSGCRA+KCLYQV+YGDG
Sbjct: 228 INWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDG 287

Query: 241 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300
           SFTVGEFVTE+L+FGNSG I NVA+GCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF
Sbjct: 288 SFTVGEFVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 347

Query: 301 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 360
           SYCLVDRDSSSSS LEFNSAAP+DSVNAPLL+SGRVDTFYYVGL G+SVGGQLLSIPP+L
Sbjct: 348 SYCLVDRDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNL 407

Query: 361 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 420
           FQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNGFALFDTCYDLSSQS
Sbjct: 408 FQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQS 467

Query: 421 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 480
           RVTIPT+SF+FAGGKSLQLPPKNYLIPVDSVGTFC AFAPTTSSLSIIGNVQQQGTRVH+
Sbjct: 468 RVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHY 527

Query: 481 DLANSLVGFSPNKC 495
           DLANS+VGFSP+KC
Sbjct: 528 DLANSVVGFSPHKC 536

BLAST of CmoCh13G010750 vs. NCBI nr
Match: XP_022924034.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 966.8 bits (2498), Expect = 6.9e-278
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA 60
           MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA
Sbjct: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA 60

Query: 61  LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE 120
           LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE
Sbjct: 61  LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE 120

Query: 121 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 180
           FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD
Sbjct: 121 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 180

Query: 181 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 240
           INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG
Sbjct: 181 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 240

Query: 241 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300
           SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF
Sbjct: 241 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300

Query: 301 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 360
           SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL
Sbjct: 301 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 360

Query: 361 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 420
           FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS
Sbjct: 361 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 420

Query: 421 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 480
           RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF
Sbjct: 421 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 480

Query: 481 DLANSLVGFSPNKC 495
           DLANSLVGFSPNKC
Sbjct: 481 DLANSLVGFSPNKC 494

BLAST of CmoCh13G010750 vs. NCBI nr
Match: KAG6584369.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019953.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 953.7 bits (2464), Expect = 6.0e-274
Identity = 490/498 (98.39%), Postives = 493/498 (99.00%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH----SSSSSAFLDVSASLKQANQVLKF 60
           MATANLLPLFSFA FFFSLLPFTLCRTSPELSH    SSSSSAFLDVSASLKQANQVLKF
Sbjct: 1   MATANLLPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSSSSAFLDVSASLKQANQVLKF 60

Query: 61  DPKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLN 120
           DPKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGH+DYKSLVLSRLDRDSSRVKSLN
Sbjct: 61  DPKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLN 120

Query: 121 DRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLD 180
           DRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVG+PAKPFYMVLD
Sbjct: 121 DRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLD 180

Query: 181 TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVA 240
           TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVA
Sbjct: 181 TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVA 240

Query: 241 YGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK 300
           YGDGSFTVGEFVTESLSFGNSGTI+NVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK
Sbjct: 241 YGDGSFTVGEFVTESLSFGNSGTISNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK 300

Query: 301 ASSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSI 360
           ASSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSI
Sbjct: 301 ASSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSI 360

Query: 361 PPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDL 420
           PPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDL
Sbjct: 361 PPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDL 420

Query: 421 SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGT 480
           SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGT
Sbjct: 421 SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGT 480

Query: 481 RVHFDLANSLVGFSPNKC 495
           RVHFDLANSLVGFSPNKC
Sbjct: 481 RVHFDLANSLVGFSPNKC 498

BLAST of CmoCh13G010750 vs. NCBI nr
Match: XP_023519500.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 952.6 bits (2461), Expect = 1.3e-273
Identity = 489/495 (98.79%), Postives = 491/495 (99.19%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH-SSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANLLPLFSFA FFFSLLPFTLCRTSPELSH SSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLLPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQDEVVP NSSSSFSLQLHSRDALQNAGH+DYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQDEVVPGNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLL+SGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLKSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGG SLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGNSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLVGFSPNKC 495
           FDLANSLVGFSPNKC
Sbjct: 481 FDLANSLVGFSPNKC 495

BLAST of CmoCh13G010750 vs. NCBI nr
Match: XP_023000890.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 940.6 bits (2430), Expect = 5.3e-270
Identity = 483/495 (97.58%), Postives = 488/495 (98.59%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH-SSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANL PLFSFA FFFSLLPFTLCRTSPELSH SSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQ+EVVP NSSSSFSLQLHSRDALQNAGH+DYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVG+PAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASL CESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSD+VNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNG ALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLVGFSPNKC 495
           FDLANSL+GFSPNKC
Sbjct: 481 FDLANSLLGFSPNKC 495

BLAST of CmoCh13G010750 vs. NCBI nr
Match: XP_038894046.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Benincasa hispida])

HSP 1 Score: 873.2 bits (2255), Expect = 1.0e-249
Identity = 442/494 (89.47%), Postives = 468/494 (94.74%), Query Frame = 0

Query: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA 60
           MAT NL PLF+ A FFFSL PFTL RTSPELS SSSSSA LDVSASL+QAN VLKFDPK 
Sbjct: 1   MATPNLPPLFAVALFFFSLFPFTLGRTSPELSRSSSSSASLDVSASLQQANHVLKFDPKP 60

Query: 61  LLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLE 120
            +SFQQQ +VVP NSS SFSLQLH RDAL NAGH+DYK+LVLSRL RDSSRVKSL DRL+
Sbjct: 61  FMSFQQQVQVVPSNSSFSFSLQLHPRDALHNAGHKDYKTLVLSRLVRDSSRVKSLYDRLQ 120

Query: 121 FSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSD 180
           F+LS+LK SDL PL+TEILPEDLSTPIVSGTSQGSGEYFSR+GVG+PAKPFYMVLDTGSD
Sbjct: 121 FALSELKSSDLHPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSD 180

Query: 181 INWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDG 240
           INWLQCQPCTDCYQQTD IFDPRTSS+FASLPCES QCQALETSGCRA+KCLYQV+YGDG
Sbjct: 181 INWLQCQPCTDCYQQTDSIFDPRTSSTFASLPCESQQCQALETSGCRASKCLYQVSYGDG 240

Query: 241 SFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300
           SFTVGEFVTE+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF
Sbjct: 241 SFTVGEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSF 300

Query: 301 SYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 360
           SYCLVDRDSSSSS LEFNSAA SDSVNAPLL+SGRVDTFYY+GL+GLSVGGQ+LSIPP+L
Sbjct: 301 SYCLVDRDSSSSSFLEFNSAASSDSVNAPLLKSGRVDTFYYIGLIGLSVGGQILSIPPNL 360

Query: 361 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQS 420
           FQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNGFALFDTCYDLSSQS
Sbjct: 361 FQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQS 420

Query: 421 RVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHF 480
           RVTIPT+SFQFAGGKSLQLPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRVHF
Sbjct: 421 RVTIPTVSFQFAGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVHF 480

Query: 481 DLANSLVGFSPNKC 495
           DLANS+VGFSPNKC
Sbjct: 481 DLANSVVGFSPNKC 494

BLAST of CmoCh13G010750 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 571.6 bits (1472), Expect = 6.0e-163
Identity = 295/495 (59.60%), Postives = 373/495 (75.35%), Query Frame = 0

Query: 16  FFSLL---PFTLCRTSPELSHSSSSSA----FLDVSASLKQANQVLKFDP--KALLSFQQ 75
           F SLL     +L  T+ + S  S S+      LDV +SL+Q   +L  DP   +L + + 
Sbjct: 6   FLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKP 65

Query: 76  QDEVVPV--NSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLS 135
           +    PV  NSSS  SL+LHSRD    + H+DYKSL LSRL+RDSSRV  +  ++ F++ 
Sbjct: 66  ESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVE 125

Query: 136 DLKRSDLQPL---QTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDI 195
            + RSDL+P+    T    EDL+TP+VSG SQGSGEYFSRIGVG PAK  Y+VLDTGSD+
Sbjct: 126 GVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDV 185

Query: 196 NWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGS 255
           NW+QC+PC DCYQQ+DP+F+P +SS++ SL C +PQC  LETS CR+ KCLYQV+YGDGS
Sbjct: 186 NWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGS 245

Query: 256 FTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFS 315
           FTVGE  T++++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+SFS
Sbjct: 246 FTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFS 305

Query: 316 YCLVDRDSSSSSALEFNSA-APSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSL 375
           YCLVDRDS  SS+L+FNS         APLLR+ ++DTFYYVGL G SVGG+ + +P ++
Sbjct: 306 YCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAI 365

Query: 376 FQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRR-TNGFALFDTCYDLSSQ 435
           F +D +G GG+I+D GTA+TRLQTQAYN+LRDAF+  T  L++ ++  +LFDTCYD SS 
Sbjct: 366 FDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSL 425

Query: 436 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 495
           S V +PT++F F GGKSL LP KNYLIPVD  GTFC AFAPT+SSLSIIGNVQQQGTR+ 
Sbjct: 426 STVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRIT 485

BLAST of CmoCh13G010750 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 564.3 bits (1453), Expect = 9.6e-161
Identity = 285/491 (58.04%), Postives = 376/491 (76.58%), Query Frame = 0

Query: 8   PLFSFASFFFSLLPFT--LCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKALLSFQ 67
           P +SF  F F L   +    R  PE   S+++++ L+V+ S+ +      F         
Sbjct: 3   PNYSFFFFIFFLTSHSSVFSRILPET--STTTTSILNVADSIHRTKYTSSF------RLN 62

Query: 68  QQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSD 127
           QQ+E    ++SSSFSLQLHSR +++   H DYKSL L+RL+RD++RVKSL  RL+ ++++
Sbjct: 63  QQEEQTH-SASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINN 122

Query: 128 LKRSDLQPLQTEILPE--DLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINW 187
           + ++DL+P+ T    E  D+  P++SGT+QGSGEYF+R+G+G+PA+  YMVLDTGSD+NW
Sbjct: 123 ISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNW 182

Query: 188 LQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFT 247
           LQC PC DCY QT+PIF+P +SSS+  L C++PQC ALE S CR A CLY+V+YGDGS+T
Sbjct: 183 LQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYT 242

Query: 248 VGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYC 307
           VG+F TE+L+ G S  + NVA+GCGH NEGLFVG+AGLLGLGGG L+L SQ+  +SFSYC
Sbjct: 243 VGDFATETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYC 302

Query: 308 LVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQM 367
           LVDRDS S+S ++F ++   D+V APLLR+ ++DTFYY+GL G+SVGG+LL IP S F+M
Sbjct: 303 LVDRDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEM 362

Query: 368 DDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVT 427
           D++G GGII+DSGTA+TRLQT+ YN+LRD+FV  T  L +  G A+FDTCY+LS+++ V 
Sbjct: 363 DESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVE 422

Query: 428 IPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLA 487
           +PT++F F GGK L LP KNY+IPVDSVGTFCLAFAPT SSL+IIGNVQQQGTRV FDLA
Sbjct: 423 VPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLA 482

Query: 488 NSLVGFSPNKC 495
           NSL+GFS NKC
Sbjct: 483 NSLIGFSSNKC 483

BLAST of CmoCh13G010750 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 6.3e-112
Identity = 245/497 (49.30%), Postives = 308/497 (61.97%), Query Frame = 0

Query: 9   LFSFASFFFSLLPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKA---LLSFQ 68
           LFS   FF SL         P  S   S       S SL  A+ V  F P +    L   
Sbjct: 9   LFSLCFFFLSL---------PSFSSLPSFQTLFPNSHSLPCASPV-SFQPDSDSESLLES 68

Query: 69  QQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSD 128
           + +      SSSS +L L   DAL +    D   L  SRL RDS RVKS+        + 
Sbjct: 69  EFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSI-------ATL 128

Query: 129 LKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQ 188
             +   + +     P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQ
Sbjct: 129 AAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQ 188

Query: 189 CQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGC--RAAKCLYQVAYGDGSFT 248
           C PC  CY Q+DPIFDPR S ++A++PC SP C+ L+++GC  R   CLYQV+YGDGSFT
Sbjct: 189 CAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFT 248

Query: 249 VGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL---TSQMKASSF 308
           VG+F TE+L+F     +  VALGCGHDNEGLFVG+AGLLGLG G LS    T       F
Sbjct: 249 VGDFSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKF 308

Query: 309 SYCLVDRDSSSS-SALEFNSAAPSDSVN-APLLRSGRVDTFYYVGLVGLSVGG-QLLSIP 368
           SYCLVDR +SS  S++ F +AA S      PLL + ++DTFYYVGL+G+SVGG ++  + 
Sbjct: 309 SYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVT 368

Query: 369 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLS 428
            SLF++D  G GG+I+DSGT++TRL   AY  +RDAF      L+R   F+LFDTC+DLS
Sbjct: 369 ASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLS 428

Query: 429 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 488
           + + V +PT+   F G   + LP  NYLIPVD+ G FC AFA T   LSIIGN+QQQG R
Sbjct: 429 NMNEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFR 484

Query: 489 VHFDLANSLVGFSPNKC 495
           V +DLA+S VGF+P  C
Sbjct: 489 VVYDLASSRVGFAPGGC 484

BLAST of CmoCh13G010750 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.5e-108
Identity = 232/482 (48.13%), Postives = 301/482 (62.45%), Query Frame = 0

Query: 31  LSHSSSSSAFLDVSASLKQANQVLKFDP-KALLSFQQQDEVVPVN---SSSSFSLQLHSR 90
           L+ S  +  F   SAS +    V+   P  A LS+ + + +   +   S++S S+ L   
Sbjct: 9   LAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTSLSVHLSHV 68

Query: 91  DALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRSDLQPLQTEILPEDLSTP 150
           DAL +        L   RL RDS RVKS+      SL+ +        +T       S  
Sbjct: 69  DALSSFSDASPADLFNLRLQRDSLRVKSIT-----SLAAVSTGRNATKRTPRTAGGFSGA 128

Query: 151 IVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSS 210
           ++SG SQGSGEYF R+GVG PA   YMVLDTGSD+ WLQC PC  CY QTD IFDP+ S 
Sbjct: 129 VISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSK 188

Query: 211 SFASLPCESPQCQAL-ETSGC---RAAKCLYQVAYGDGSFTVGEFVTESLSFGNSGTINN 270
           +FA++PC S  C+ L ++S C   R+  CLYQV+YGDGSFT G+F TE+L+F +   +++
Sbjct: 189 TFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTF-HGARVDH 248

Query: 271 VALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK---ASSFSYCLVDRDSSSSSA----- 330
           V LGCGHDNEGLFVG+AGLLGLG G LS  SQ K      FSYCLVDR SS SS+     
Sbjct: 249 VPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPST 308

Query: 331 -LEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGG-QLLSIPPSLFQMDDAGYGGII 390
            +  N+A P  SV  PLL + ++DTFYY+ L+G+SVGG ++  +  S F++D  G GG+I
Sbjct: 309 IVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVI 368

Query: 391 VDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTIPTLSFQFA 450
           +DSGT++TRL   AY  LRDAF      L+R   ++LFDTC+DLS  + V +PT+ F F 
Sbjct: 369 IDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFG 428

Query: 451 GGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLVGFSPN 495
           GG+ + LP  NYLIPV++ G FC AFA T  SLSIIGN+QQQG RV +DL  S VGF   
Sbjct: 429 GGE-VSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSR 483

BLAST of CmoCh13G010750 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.2e-105
Identity = 214/497 (43.06%), Postives = 295/497 (59.36%), Query Frame = 0

Query: 6   LLPLFSFASFFFSLLPFTLCRTS----PELSHSSSSSAFLDVSASLKQANQVLKFDPKAL 65
           LLPLF    FFF  L   L  +S    P+          L V+A+L   N     D    
Sbjct: 2   LLPLF----FFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSD---- 61

Query: 66  LSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEF 125
                        SSS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+  
Sbjct: 62  ------------ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISG 121

Query: 126 SLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDI 185
            +  +  SD     +     D  + IVSG  QGSGEYF RIGVG P +  YMV+D+GSD+
Sbjct: 122 KV--IPSSD-----SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDM 181

Query: 186 NWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGS 245
            W+QCQPC  CY+Q+DP+FDP  S S+  + C S  C  +E SGC +  C Y+V YGDGS
Sbjct: 182 VWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGS 241

Query: 246 FTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK---AS 305
           +T G    E+L+F  +  + NVA+GCGH N G+F+G+AGLLG+GGGS+S   Q+      
Sbjct: 242 YTKGTLALETLTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGG 301

Query: 306 SFSYCLVDRDSSSSSALEF-NSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIP 365
           +F YCLV R + S+ +L F   A P  +   PL+R+ R  +FYYVGL GL VGG  + +P
Sbjct: 302 AFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLP 361

Query: 366 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLS 425
             +F + + G GG+++D+GTA+TRL T AY   RD F S+T  L R +G ++FDTCYDLS
Sbjct: 362 DGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLS 421

Query: 426 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 485
               V +PT+SF F  G  L LP +N+L+PVD  GT+C AFA + + LSIIGN+QQ+G +
Sbjct: 422 GFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQ 470

Query: 486 VHFDLANSLVGFSPNKC 495
           V FD AN  VGF PN C
Sbjct: 482 VSFDGANGFVGFGPNVC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LS408.5e-16259.60Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ38.9e-11149.30Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LHE31.6e-10443.06Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q766C31.9e-7344.67Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C21.3e-7238.24Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1E8153.3e-278100.00protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KJJ82.6e-27097.58protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1C6K99.2e-24488.98protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN... [more]
A0A5D3BI723.9e-24287.25Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3C5F63.9e-24287.25protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
Match NameE-valueIdentityDescription
XP_022924034.16.9e-278100.00protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata][more]
KAG6584369.16.0e-27498.39Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp... [more]
XP_023519500.11.3e-27398.79protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo][more]
XP_023000890.15.3e-27097.58protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima][more]
XP_038894046.11.0e-24989.47protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G18490.16.0e-16359.60Eukaryotic aspartyl protease family protein [more]
AT1G25510.19.6e-16158.04Eukaryotic aspartyl protease family protein [more]
AT1G01300.16.3e-11249.30Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.5e-10848.13Eukaryotic aspartyl protease family protein [more]
AT3G20015.11.2e-10543.06Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 158..317
e-value: 2.2E-48
score: 164.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 317..494
e-value: 5.6E-51
score: 174.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 133..316
e-value: 2.8E-50
score: 173.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 151..494
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 339..490
e-value: 1.4E-36
score: 125.7
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 13..494
NoneNo IPR availablePANTHERPTHR13683:SF274PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1coord: 13..494
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 173..184
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 158..490
score: 48.286057

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G010750.1CmoCh13G010750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity