CmoCh13G009720 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G009720
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCmo_Chr13: 8374833 .. 8376008 (-)
RNA-Seq ExpressionCmoCh13G009720
SyntenyCmoCh13G009720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGGAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTGTTCTGGTAAATTTCTTCCCTTCCTTCATTCTGCCTTTATTTCTCACTTTCAGAGCTAACCCACATACTATTTTGCAGTGAAAACCTCAAATCCTACGCAGAATTGGTTTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATCAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCATCATGGCTGGAGATGCCTTCTTTGTAAGTTTCCATTAACTTCAAATTCCATTACAACTAGCAATAACCCATCAAAAACTTACAAACAATTGTGGGGTTGTTGGGACAGGCGTGGAGCAATGGGAGGATATACTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCACAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCCTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAACTATTAACCCATCAAATTCCCATTTTCAG

mRNA sequence

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGGAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTTGAAAACCTCAAATCCTACGCAGAATTGGTTTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATCAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCATCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATACTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCACAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCCTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAACTATTAACCCATCAAATTCCCATTTTCAG

Coding sequence (CDS)

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATTTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGCCATGGAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTTGAAAACCTCAAATCCTACGCAGAATTGGTTTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGGGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATCAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTGAATCTTCTGCCAAATCATTCATCATCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATACTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCACAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCCTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

Protein sequence

MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQSSPSAIKAYCGVANGLLDSRTCIPMH
Homology
BLAST of CmoCh13G009720 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.3e-65
Identity = 128/316 (40.51%), Postives = 195/316 (61.71%), Query Frame = 0

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW   +  V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP--L 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI+D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP-----HRNEM 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P        E 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWS 243
            LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRF 303
           NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTEKGQSSPSAIKAY 311
           + TE G+ + SA+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 318

BLAST of CmoCh13G009720 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 7.0e-64
Identity = 128/317 (40.38%), Postives = 193/317 (60.88%), Query Frame = 0

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW      V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP--L 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP------HRNE 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P         E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 311
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

BLAST of CmoCh13G009720 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.3e-61
Identity = 124/306 (40.52%), Postives = 185/306 (60.46%), Query Frame = 0

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW      V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 65

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP--L 123
           E+P+  K +NVS K FHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP----HRNEMN 183
           E +  ++E + +L+  V++M+ ES+G++     H  ST YL R++KY  P       E  
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 184 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSN 243
           LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A  N
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLN 245

Query: 244 GRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRFY 302
           GR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F+
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 301

BLAST of CmoCh13G009720 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 9.5e-53
Identity = 113/307 (36.81%), Postives = 176/307 (57.33%), Query Frame = 0

Query: 5   LPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFE 64
           LPV+  S  NL+ G   W S R +VREALEEYGCF A+ D+VS E     +     E+  
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQK-AVCNKGHELLN 68

Query: 65  VPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWP-SRPNPLEN 124
           +PLE K+ N ++  + G+     +  ++E MG+        ++ F+NLMWP    +  E 
Sbjct: 69  LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWPEGNKDFCET 128

Query: 125 LKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPH 184
           + SY + +++++  +K MVFES+G++  F S  +ST YLL  ++Y+ P ++  +     H
Sbjct: 129 VYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSLH 188

Query: 185 TDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIYSA 244
            DKS LTI++QN V GL  +T+D  W+         +A + I++ GD F AWSNGR++S 
Sbjct: 189 KDKSILTIVNQNDVKGLEFETKDGEWI-------LPTADNHIVLLGDCFMAWSNGRLHSP 248

Query: 245 PHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQSS 304
            HRVT+  N+ R S   FSF   I++ P ELVD++HP  F  F+  + L +  T++G  +
Sbjct: 249 LHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKA 304

Query: 305 PSAIKAY 311
              +K Y
Sbjct: 309 VCDLKQY 304

BLAST of CmoCh13G009720 vs. ExPASy Swiss-Prot
Match: Q945B4 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.8e-51
Identity = 127/412 (30.83%), Postives = 194/412 (47.09%), Query Frame = 0

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LP++  S   L+ G  KW   R  VR+ALE+YGCF A  D VSME     +++AM+E+F
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQG-SVLKAMQELF 66

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLEN 123
            +P E K +NV  KPF GYF  N L    ES GI+D  +      F+  +WP     ++ 
Sbjct: 67  ALPTEAKQRNVCPKPFAGYFSHNGL---SESFGIKDANILEKAHEFTQQLWPEGNKSIKM 126

Query: 124 LKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEM------- 183
           ++ YAE +++L+  V++++ ESYG++     H  ST Y +R++KY     N++       
Sbjct: 127 IQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVGAN 186

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 187 VDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDLDA 246

Query: 244 -----------------------------NLGAFPHTDKSFLTILHQNSVNGLHIKTRDN 303
                                         LG   HTDKS   I++Q+ ++GL +KT++ 
Sbjct: 247 EANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDGLEVKTKEG 306

Query: 304 RWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIYSAPHRVTMSGNRE-RYSVGLFSF-ND 317
           +W+  +P     +  + I++AGDA  A  NGRI S  HRV ++  ++ RY+  LFS+  +
Sbjct: 307 KWIRVKP-----APNTVIVIAGDALCALMNGRIPSPYHRVRVTERKKTRYAAALFSYPKE 366

BLAST of CmoCh13G009720 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.4e-187
Identity = 327/329 (99.39%), Postives = 327/329 (99.39%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L  ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 1.7e-185
Identity = 323/329 (98.18%), Postives = 326/329 (99.09%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGE+PVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMMEAM+
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L  ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.2e-184
Identity = 318/329 (96.66%), Postives = 326/329 (99.09%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASS+KMMEAM+
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L   NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSA+SSA+SFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 8.5e-137
Identity = 239/326 (73.31%), Postives = 277/326 (84.97%), Query Frame = 0

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LP+V+FS  NL    R W STREKVREALEEYGCFVAL DSVSME SS  M ++++E+F
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSS-NMFDSLKELF 64

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP--L 123
           EVPLERK++NVSEKP+HGYFGQNPLMPIHESMGIEDP L S  QSF+NLMWPS  N    
Sbjct: 65  EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFC 124

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183
           EN+ SYA  +++L++TVKKMVFESYG++    SH +ST+YLLRMIKYRVP +NEMN+GAF
Sbjct: 125 ENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAF 184

Query: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIY 243
           PHTDKSFLTILHQN VNGL IKT+ N+W++Y P S+ SS  SFI+MAGDAFFAWSNGRIY
Sbjct: 185 PHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGRIY 244

Query: 244 SAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQ 303
           S PH+V MSG RERYSVGLFSFNDGIVQIPKELVD+ HPQQFK FDHQDYLRFYSTE+GQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 304 SSPSAIKAYCGVANGLLDSRTCIPMH 328
           +SP AIKAYCG+ NGL+D+R CI +H
Sbjct: 305 NSPCAIKAYCGIGNGLVDTRGCILLH 325

BLAST of CmoCh13G009720 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 5.7e-133
Identity = 237/329 (72.04%), Postives = 279/329 (84.80%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           M+ ELPVV+F S NL+SG ++W  T +KVREALE+YGCFVAL DSVS E SS  M ++++
Sbjct: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSS-NMFDSLK 64

Query: 61  EVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
           E+F +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124

Query: 121 P--LENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180
               E++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184

Query: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSN 240
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDAFFAWSN
Sbjct: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244

Query: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIP+ELVDDKHPQ F  FDHQD+LRFYST
Sbjct: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYST 304

Query: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 327
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPL 331

BLAST of CmoCh13G009720 vs. NCBI nr
Match: XP_022923887.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 665.2 bits (1715), Expect = 2.8e-187
Identity = 327/329 (99.39%), Postives = 327/329 (99.39%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L  ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. NCBI nr
Match: KAG6584272.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 660.6 bits (1703), Expect = 7.0e-186
Identity = 323/329 (98.18%), Postives = 326/329 (99.09%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAM+
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L  ENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. NCBI nr
Match: XP_022923888.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_023519885.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo] >XP_023519886.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658.3 bits (1697), Expect = 3.5e-185
Identity = 323/329 (98.18%), Postives = 326/329 (99.09%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGE+PVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMMEAM+
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L  ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. NCBI nr
Match: KAG7019869.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 656.8 bits (1693), Expect = 1.0e-184
Identity = 320/329 (97.26%), Postives = 325/329 (98.78%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGELPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMME M+
Sbjct: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L  ENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. NCBI nr
Match: KAG6584273.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 656.4 bits (1692), Expect = 1.3e-184
Identity = 322/329 (97.87%), Postives = 325/329 (98.78%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60
           MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMMEAM+
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 L--ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           L  ENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 328
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

BLAST of CmoCh13G009720 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.4e-75
Identity = 136/312 (43.59%), Postives = 206/312 (66.03%), Query Frame = 0

Query: 5   LPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFE 64
           LPV+ FS+ NL+ G  +W  TR  V++AL++YG F A  D +  E     +  A+EE+F+
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK-SVFGALEELFD 71

Query: 65  VPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLE-N 124
           +PL+ K++NVS+KPFHGY GQ P++P++ESMGI+D  +   + +F+  +WP         
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFSTT 131

Query: 125 LKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPH 184
           ++S+++ +S+L+ T+++M+ ES+GL      H  ST YLLR++KY+ P   E  +G   H
Sbjct: 132 IQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNAH 191

Query: 185 TDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIYSA 244
           TDK+ +TIL+QN V GL ++T+D  W++ +P     +  SF +M GD+ +A  NGR++S 
Sbjct: 192 TDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-----TQDSFTVMIGDSLYALLNGRLHSP 251

Query: 245 PHRVTMSGNRERYSVGLFSFNDG--IVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQ 304
            HRV M+G   RYS+GLFS      IV  P ELVD++HP+ FK FDH ++L+FY TE GQ
Sbjct: 252 YHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQ 311

Query: 305 SSPSAIKAYCGV 314
            S SA+K YCG+
Sbjct: 312 RSQSALKTYCGI 317

BLAST of CmoCh13G009720 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.5e-74
Identity = 135/314 (42.99%), Postives = 205/314 (65.29%), Query Frame = 0

Query: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVA-LCDSVSMEASSLKMMEAM 60
           M+ ++P + FS  +L+ G + W STRE +R+ALEEYGCF+  L D   ++    ++  ++
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLD-RVFGSL 60

Query: 61  EEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWP-SRP 120
            ++F++P + K++N  +KP +GY GQ P +P+HES+GI++       +SF+ LMWP    
Sbjct: 61  VDLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNE 120

Query: 121 NPLENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           +  E L  YAE  ++L+Q V +MVF+SY ++  +  + +ST YLLR++K R P+     L
Sbjct: 121 HFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240
           G   HTDKSF TILHQ+ VNGL ++TR+   +        SS   F+++AGDA  AWSN 
Sbjct: 181 GFVTHTDKSFTTILHQDQVNGLEMETREGERININ----LSSPSLFMVVAGDALMAWSND 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           R++S  H+V +SG  +RYS+G+F+FN+G +Q+P+EL+D +HP  +K FDH   LRFY T+
Sbjct: 241 RVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTD 300

Query: 301 KGQSSPSAIKAYCG 313
            G  S   IKAYCG
Sbjct: 301 TGYKSECPIKAYCG 309

BLAST of CmoCh13G009720 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-73
Identity = 134/305 (43.93%), Postives = 201/305 (65.90%), Query Frame = 0

Query: 11  SSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFEVPLERK 70
           S   L+     W S   +  EALEEYGCF+A+ D V+ +     +  A EE+F++P E K
Sbjct: 17  SQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDD-SIFAAAEELFDLPTETK 76

Query: 71  VQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLEN-LKSYAE 130
            +NV+EKP+HGY GQ P++P+HE +G++        Q F++LMWP   +   N + +++ 
Sbjct: 77  KKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCNTVHTFSN 136

Query: 131 LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP-HTDKSF 190
            V++L++ V +M+FE+YG++  + SH  S  YLL+ +KY  P  + +++ AFP HTDK+F
Sbjct: 137 AVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPAFPQHTDKTF 196

Query: 191 LTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRIYSAPHRVT 250
           L+ILHQN VNGL +K++D  W+  Q P      KS+++MAGD    WSN RI S  HRVT
Sbjct: 197 LSILHQNDVNGLEVKSKDGEWISLQLP-----PKSYVVMAGDISMGWSNDRIRSCEHRVT 256

Query: 251 MSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQSSPSAIK 310
           M G++ RY++GLFSF   +V IP+ELVDDKHP  +K FD+   + FY+T++G+ + S +K
Sbjct: 257 MEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANSTLK 314

Query: 311 AYCGV 314
           AYCG+
Sbjct: 317 AYCGI 314

BLAST of CmoCh13G009720 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 245.7 bits (626), Expect = 5.0e-65
Identity = 128/317 (40.38%), Postives = 193/317 (60.88%), Query Frame = 0

Query: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63
           +LPV+ FS  NL+ G  KW      V +ALE+YGCF A  D +S+E +   + EAME++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP--L 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP------HRNE 183
           E +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P         E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S ++M GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 311
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

BLAST of CmoCh13G009720 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 127/316 (40.19%), Postives = 187/316 (59.18%), Query Frame = 0

Query: 5   LPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVFE 64
           LP++ FSS  L  G   W +TR+ VR A+E  G FVA    VS +     ++  M+E++ 
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRD-NLLAGMKEMYY 68

Query: 65  VPLERKVQNVSEKPFHGYFGQ-NPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPL-E 124
           +P + K++N + K  HGY         IHES+GI+        + FS L+WP   +P  +
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGNDPFCQ 128

Query: 125 NLKSYAELVSQLEQTVKKMVFESYGL--KSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 184
               YA  +++L+QTV +M++ESYG+  K   +SH++ST+YLLRM+ YR     E N G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 185 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 244
             HTDKSF++ILHQN V GL +KT   +WV + P     S   F++++G    AWSN RI
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP-----SPTRFVVLSGMGLTAWSNDRI 248

Query: 245 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST--- 304
            +  H+V MS +  RYS+G FSF+ G ++ P+ELVDD+HP ++  F+H   LRFY +   
Sbjct: 249 KACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYIN 308

Query: 305 EKGQSSPSAIKAYCGV 314
              +SS   ++ YCGV
Sbjct: 309 SLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q945B68.3e-6540.51Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA37.0e-6440.38Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q944Z54.3e-6140.52Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ369.5e-5336.81Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q945B41.8e-5130.832-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
A0A6J1E7C81.4e-18799.39probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1EAT71.7e-18598.18probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1KFF73.2e-18496.66probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1C7128.5e-13773.31probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara... [more]
A0A5D3BIZ75.7e-13372.04Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
XP_022923887.12.8e-18799.39probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata][more]
KAG6584272.17.0e-18698.18putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros... [more]
XP_022923888.13.5e-18598.18probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_0235... [more]
KAG7019869.11.0e-18497.26putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros... [more]
KAG6584273.11.3e-18497.87putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
AT1G52820.12.4e-7543.592-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52790.13.5e-7442.992-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.11.3e-7343.932-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G03070.15.0e-6540.382-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.12.6e-6140.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 168..263
e-value: 1.8E-17
score: 63.7
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..294
e-value: 2.6E-50
score: 173.8
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 5..84
e-value: 2.0E-6
score: 28.5
NoneNo IPR availablePANTHERPTHR47990:SF172-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKEcoord: 4..312
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 4..312
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 4..300
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 159..266
score: 10.434196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G009720.1CmoCh13G009720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding