CmoCh13G009690 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G009690
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionProtein DETOXIFICATION
LocationCmo_Chr13: 8358801 .. 8361779 (+)
RNA-Seq ExpressionCmoCh13G009690
SyntenyCmoCh13G009690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCAGTCTTGTTTAAAAGCTTGATTGTTGTCTCTGTACATAGAAAGTTATATCAGGCCTCTGTTTCGGCGGAGATGGAGGCGGCGCCACTTCTGGGTGACAGCAGCAGCGGAGGAGACTATGCTCCGGCGAGGAACTTTGAGCAGTTCAAGCGTATCGTGTGGAGTGAGACGGTGAAGACTTGGACCATCTCCGGTCCGGTCATTTTTCAGATCGTTTGTCAATATGGAACCAACTCTGTTACCAATATCTTTGTGGGTCAACTCGGGGAGCTTGAGCTCTCTGGCGTTTCCATTGCCATCTCTGTTATTGCCACTTTCGCTTTCGGTTTCATGGTCTGTATTTTCTCTCTCAATCCCCATTGATTGAAAAGCTAGATTCTGATGTGTGTGTTTAAAAATTGTTCACAGTTTGGAATGGGAAGTGCAACAGAGACACTGTGCGGCCAAGCATTTGGGGCTGGACAAATCCACATGCTGGGTGTGTATATGCAGAGATCATGGGTCATTATGTTCGTATGTGCCTTAATAATCACACCAGTCTATGTCTTTGCCACTCCCATTTTGAAGCTTTTGGGGCAACAAGATGATGTCGCAGAACTGGCTGGGAATTTCTCTCTGCTCATACTCCCACAGTTGTTCTCCTTTGTGTTGGCTTTTCCAACCCAAAAGTTTCTGCAGGCACAGAGCAAAGTGTGGGCGTTGGCCTGGATTGGCTTTGGGGCTCTTCTGGCTCATGTTTTCATGTTGTGGCTGTTCATTTTTGAGTTCGGTTGGAGCACCACTGGGGCTGGTCTGGCTTTGAACATCTCTGGGTGGGGGCTTTCCATTGCCCAAGTAATTTATGTGATGGGTTGGTGTAGAGATGCTTGGCAGGGATTCTCTTGGTTGGCTTTTAAGGATTTGTGGGGATTTGTTAAGCTCTCGTTTTCCTCTGCTATTATGTTCTGCTTGGAGATTTGGTACATGAGTAGCATCATTGTTCTTGCTGGCCACCTTCCAAATGCTGTCATTTCTGTTGATTCACTTTCGATTTGGTAAGTAGATAGTTCTTTGGATGTTTGCGTGGTTATGTTCTTCGTTTTTCATGGATCTTAAGTTTTGGGTTGTTTCAGCATGAACTTGAACGGATGGGAAAATATCATTTTCATTGGAATCAATGTCGCCATGAGGTAATGAATGATCTTGAACTATTTTAAATCTTTTAGATTCATGAAACTGTTGTTTAGATCTCTTAAATGGTATCATCAAGTTCATGAAACTGTTCTTTAGATCTCTTAAATGACATCATACCAATAGAGATCTGTAATAGTCTAAACCCACCACTACAGATATTGTCATCTTTAGGCATTCCGTTCAACTTCTCCTCAAGGTTTTAAAACATGTCTTTCGTAGTTAAACAACTGAACCAGCACTCATCCAAATACTGTCCTCCTTGGGCTTTCCCTCTCGGGCTTCCCGTTATACGTATGCTAGGAACAGGTTTTCACATCCTTATAAAGAATGCTACGTTTCCTCCCCAACCGATATGGGATCTCACAATCCACCCCCTTTGGGTCTCAACCTTCTCGCTGGCACATCGCCCGGTGTCTGGCTCTGATTTAGGTGTGGTATTCAAATGGTATCAAATGGCTCTGATCCAAATGTGGAATCCTCACTGACATTCAAATGGTATCAAAGCCAGACACTGAGCGATATGCCAGCAAGGACGTTAGGCTTCTAAAGGGGGTGGATTGAGAGATCCCACAACGGTTGAAGAGGGAAACGAAGCATTCTTTATAAGGGTGTGGAAATCTCTACCTAATAGACATGTTTTAAAAACTTTGAAGGAAAGCCTGAAAGAAAAAACCAAAAGAGGACAATATCTACAAGCACTGGGTTTGGGATGTTACAATAGATCTGTCCTACAATTGTTTGAAAAGCTTTAATCTTTCATAATATTCATCAGAATCTGAGAGAGGATCCATTTTAGGCCCATTTCTTATACTTATGTTCTTCTGTTGTTTGTTTCCAGTGTTAGGGTCTCTAATGAACTTGGAAAAGCACGGCCTCGAGCTGCGAAGTACTCTGTTTATGTGACAATCGCAGAATCTCTTATTCTGGGGCTCCTTTTTATGGTGCTGATATTCTTTGTAAAGGATCATTTTGCTGTCATCTTTACAAGCAGTGTAGCTGTGCAGAAATATGTTGCCAAATTAGCCTATCTTCTTGGCATAACCATGGTTCTCAACAGTGTCCAACCAGTCATATCAGGTGAACTAATAATCTCTCTCTCTATATAAATATGTTAGTGAAGAAGTGGTTTTGAATGAGCCTTTACAATCCTCTGGGCCTTCCTTGTTCAGGTGTGGCCATTGGAGCTGGATGGCAGGCATTGGTGGCTTACATAAACTTAGGCTGCTACTACATTTTTGGTCTCCCTCTTGGGATTGTCTTAGGTTACGTAGCAAAGTTTGGAGTGAAGGTATGCCAAAGAAAACTAGCCAATCAAGATCGAGTTTAAGAGTGGCTTCTTAATCTAACACTTTTGGTTGGGTGCAGGGGCTTTGGGGCGGAATGATAGCCGGGATAGCAATGCAGACGATTCTGCTGCTGCTTGTTCTGTACAAAACCAACTGGAATAAAGAGGTAGGAGAAACCAAATGTTTTTCTTTTTCTTTTTTTGTTTTTTGGGTGTTGTAGGGAAGGAAGGAAGGTCGGAGCAGTTTTAATGGCGAATTGTTGAATGATTATTGCAGGTGGAGGAAACTTCAGGAAGGATGCAGAAATGGACTGGACAGGACATTGGAACAGAGAGGAGAGCAGAAAGCTAAAAGAAGTGTCATTGGGATGTCTCCATCCCCAACCGCTGCAACCTTTGTCTTTCTTTCTTTTTTCTGTATTTTTGGCTGAGGACTTTTCATTTCGTTTTTAAGTTGAGGGGTTGTAATAAACTCGGCCGTTTCTGATCTTGACACAAATAATATGGATTAGGTTGGAA

mRNA sequence

CATCAGTCTTGTTTAAAAGCTTGATTGTTGTCTCTGTACATAGAAAGTTATATCAGGCCTCTGTTTCGGCGGAGATGGAGGCGGCGCCACTTCTGGGTGACAGCAGCAGCGGAGGAGACTATGCTCCGGCGAGGAACTTTGAGCAGTTCAAGCGTATCGTGTGGAGTGAGACGGTGAAGACTTGGACCATCTCCGGTCCGGTCATTTTTCAGATCGTTTGTCAATATGGAACCAACTCTGTTACCAATATCTTTGTGGGTCAACTCGGGGAGCTTGAGCTCTCTGGCGTTTCCATTGCCATCTCTGTTATTGCCACTTTCGCTTTCGGTTTCATGTTTGGAATGGGAAGTGCAACAGAGACACTGTGCGGCCAAGCATTTGGGGCTGGACAAATCCACATGCTGGGTGTGTATATGCAGAGATCATGGGTCATTATGTTCGTATGTGCCTTAATAATCACACCAGTCTATGTCTTTGCCACTCCCATTTTGAAGCTTTTGGGGCAACAAGATGATGTCGCAGAACTGGCTGGGAATTTCTCTCTGCTCATACTCCCACAGTTGTTCTCCTTTGTGTTGGCTTTTCCAACCCAAAAGTTTCTGCAGGCACAGAGCAAAGTGTGGGCGTTGGCCTGGATTGGCTTTGGGGCTCTTCTGGCTCATGTTTTCATGTTGTGGCTGTTCATTTTTGAGTTCGGTTGGAGCACCACTGGGGCTGGTCTGGCTTTGAACATCTCTGGGTGGGGGCTTTCCATTGCCCAAGTAATTTATGTGATGGGTTGGTGTAGAGATGCTTGGCAGGGATTCTCTTGGTTGGCTTTTAAGGATTTGTGGGGATTTGTTAAGCTCTCGTTTTCCTCTGCTATTATGTTCTGCTTGGAGATTTGGTACATGAGTAGCATCATTGTTCTTGCTGGCCACCTTCCAAATGCTGTCATTTCTGTTGATTCACTTTCGATTTGCATGAACTTGAACGGATGGGAAAATATCATTTTCATTGGAATCAATGTCGCCATGAGTGTTAGGGTCTCTAATGAACTTGGAAAAGCACGGCCTCGAGCTGCGAAGTACTCTGTTTATGTGACAATCGCAGAATCTCTTATTCTGGGGCTCCTTTTTATGGTGCTGATATTCTTTGTAAAGGATCATTTTGCTGTCATCTTTACAAGCAGTGTAGCTGTGCAGAAATATGTTGCCAAATTAGCCTATCTTCTTGGCATAACCATGGTTCTCAACAGTGTCCAACCAGTCATATCAGGTGTGGCCATTGGAGCTGGATGGCAGGCATTGGTGGCTTACATAAACTTAGGCTGCTACTACATTTTTGGTCTCCCTCTTGGGATTGTCTTAGGTTACGTAGCAAAGTTTGGAGTGAAGGGGCTTTGGGGCGGAATGATAGCCGGGATAGCAATGCAGACGATTCTGCTGCTGCTTGTTCTGTACAAAACCAACTGGAATAAAGAGGTGGAGGAAACTTCAGGAAGGATGCAGAAATGGACTGGACAGGACATTGGAACAGAGAGGAGAGCAGAAAGCTAAAAGAAGTGTCATTGGGATGTCTCCATCCCCAACCGCTGCAACCTTTGTCTTTCTTTCTTTTTTCTGTATTTTTGGCTGAGGACTTTTCATTTCGTTTTTAAGTTGAGGGGTTGTAATAAACTCGGCCGTTTCTGATCTTGACACAAATAATATGGATTAGGTTGGAA

Coding sequence (CDS)

ATGGAGGCGGCGCCACTTCTGGGTGACAGCAGCAGCGGAGGAGACTATGCTCCGGCGAGGAACTTTGAGCAGTTCAAGCGTATCGTGTGGAGTGAGACGGTGAAGACTTGGACCATCTCCGGTCCGGTCATTTTTCAGATCGTTTGTCAATATGGAACCAACTCTGTTACCAATATCTTTGTGGGTCAACTCGGGGAGCTTGAGCTCTCTGGCGTTTCCATTGCCATCTCTGTTATTGCCACTTTCGCTTTCGGTTTCATGTTTGGAATGGGAAGTGCAACAGAGACACTGTGCGGCCAAGCATTTGGGGCTGGACAAATCCACATGCTGGGTGTGTATATGCAGAGATCATGGGTCATTATGTTCGTATGTGCCTTAATAATCACACCAGTCTATGTCTTTGCCACTCCCATTTTGAAGCTTTTGGGGCAACAAGATGATGTCGCAGAACTGGCTGGGAATTTCTCTCTGCTCATACTCCCACAGTTGTTCTCCTTTGTGTTGGCTTTTCCAACCCAAAAGTTTCTGCAGGCACAGAGCAAAGTGTGGGCGTTGGCCTGGATTGGCTTTGGGGCTCTTCTGGCTCATGTTTTCATGTTGTGGCTGTTCATTTTTGAGTTCGGTTGGAGCACCACTGGGGCTGGTCTGGCTTTGAACATCTCTGGGTGGGGGCTTTCCATTGCCCAAGTAATTTATGTGATGGGTTGGTGTAGAGATGCTTGGCAGGGATTCTCTTGGTTGGCTTTTAAGGATTTGTGGGGATTTGTTAAGCTCTCGTTTTCCTCTGCTATTATGTTCTGCTTGGAGATTTGGTACATGAGTAGCATCATTGTTCTTGCTGGCCACCTTCCAAATGCTGTCATTTCTGTTGATTCACTTTCGATTTGCATGAACTTGAACGGATGGGAAAATATCATTTTCATTGGAATCAATGTCGCCATGAGTGTTAGGGTCTCTAATGAACTTGGAAAAGCACGGCCTCGAGCTGCGAAGTACTCTGTTTATGTGACAATCGCAGAATCTCTTATTCTGGGGCTCCTTTTTATGGTGCTGATATTCTTTGTAAAGGATCATTTTGCTGTCATCTTTACAAGCAGTGTAGCTGTGCAGAAATATGTTGCCAAATTAGCCTATCTTCTTGGCATAACCATGGTTCTCAACAGTGTCCAACCAGTCATATCAGGTGTGGCCATTGGAGCTGGATGGCAGGCATTGGTGGCTTACATAAACTTAGGCTGCTACTACATTTTTGGTCTCCCTCTTGGGATTGTCTTAGGTTACGTAGCAAAGTTTGGAGTGAAGGGGCTTTGGGGCGGAATGATAGCCGGGATAGCAATGCAGACGATTCTGCTGCTGCTTGTTCTGTACAAAACCAACTGGAATAAAGAGGTGGAGGAAACTTCAGGAAGGATGCAGAAATGGACTGGACAGGACATTGGAACAGAGAGGAGAGCAGAAAGCTAA

Protein sequence

MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIFVGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVIMFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQSKVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDAWQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLNGWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFAVIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLPLGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIGTERRAES
Homology
BLAST of CmoCh13G009690 vs. ExPASy Swiss-Prot
Match: F4JTB3 (Protein DETOXIFICATION 35 OS=Arabidopsis thaliana OX=3702 GN=DTX35 PE=1 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.5e-187
Identity = 325/479 (67.85%), Postives = 392/479 (81.84%), Query Frame = 0

Query: 4   APLLGDSSSGG----DYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNI 63
           APLL   + GG    DYAPAR++   KR++ +E+ K W I+ PV F I+CQYG +SVTNI
Sbjct: 5   APLL---THGGEVEEDYAPARSWTDVKRVLSTESAKLWMIAAPVGFNIICQYGVSSVTNI 64

Query: 64  FVGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWV 123
           FVG +GE+ELS VSI++SVI TF+FGF+ GMGSA ETLCGQA+GAGQ++MLGVYMQRSW+
Sbjct: 65  FVGHIGEVELSAVSISLSVIGTFSFGFLLGMGSALETLCGQAYGAGQVNMLGVYMQRSWI 124

Query: 124 IMFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQ 183
           I+FV    + P+Y+FATP+L+LLGQ +++A  AG F+LL +PQLFS    FPT KFLQAQ
Sbjct: 125 ILFVSCFFLLPIYIFATPVLRLLGQAEEIAVPAGQFTLLTIPQLFSLAFNFPTSKFLQAQ 184

Query: 184 SKVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRD 243
           SKV A+AWIGF AL  HV MLWLFI EFGW T GA LA NI+ WG +IAQ++YV+GWC +
Sbjct: 185 SKVVAIAWIGFVALSLHVIMLWLFIIEFGWGTNGAALAFNITNWGTAIAQIVYVIGWCNE 244

Query: 244 AWQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNL 303
            W G SWLAFK++W FV+LS +SA+M CLEIWYM SIIVL G L NAVI+VDSLSICMN+
Sbjct: 245 GWTGLSWLAFKEIWAFVRLSIASAVMLCLEIWYMMSIIVLTGRLDNAVIAVDSLSICMNI 304

Query: 304 NGWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHF 363
           NG E ++FIGIN A+SVRVSNELG  RPRAAKYSVYVT+ +SL++GL+FMV I   +DHF
Sbjct: 305 NGLEAMLFIGINAAISVRVSNELGLGRPRAAKYSVYVTVFQSLLIGLVFMVAIIIARDHF 364

Query: 364 AVIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGL 423
           A+IFTSS  +Q+ V+KLAYLLGITMVLNSVQPV+SGVA+G GWQ LVAYINLGCYYIFGL
Sbjct: 365 AIIFTSSKVLQRAVSKLAYLLGITMVLNSVQPVVSGVAVGGGWQGLVAYINLGCYYIFGL 424

Query: 424 PLGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQD 479
           P G +LGY+A FGV GLW GMIAG A+QT+LLL+VLYKTNWNKEVEET  RM+KW G +
Sbjct: 425 PFGYLLGYIANFGVMGLWSGMIAGTALQTLLLLIVLYKTNWNKEVEETMERMKKWGGSE 480

BLAST of CmoCh13G009690 vs. ExPASy Swiss-Prot
Match: F4JH46 (Protein DETOXIFICATION 34 OS=Arabidopsis thaliana OX=3702 GN=DTX34 PE=2 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 3.6e-165
Identity = 280/469 (59.70%), Postives = 369/469 (78.68%), Query Frame = 0

Query: 6   LLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIFVGQLG 65
           LLG+++   D+ P ++F   K +   ET K W I+ P+ F I+C YG NS T+IFVG +G
Sbjct: 62  LLGETTGDADFPPIQSFRDAKLVCVVETSKLWEIAAPIAFNILCNYGVNSFTSIFVGHIG 121

Query: 66  ELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVIMFVCA 125
           +LELS V+IA+SV++ F+FGF+ GM SA ETLCGQAFGAGQ+ MLGVYMQRSW+I+   +
Sbjct: 122 DLELSAVAIALSVVSNFSFGFLLGMASALETLCGQAFGAGQMDMLGVYMQRSWLILLGTS 181

Query: 126 LIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQSKVWAL 185
           + + P+Y++ATP+L LLGQ+ ++AE++G F+  I+PQ+F+  + FPTQKFLQ+QSKV  +
Sbjct: 182 VCLLPLYIYATPLLILLGQEPEIAEISGKFTTQIIPQMFALAINFPTQKFLQSQSKVGIM 241

Query: 186 AWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDAWQGFS 245
           AWIGF AL  H+F+L+LFI  F W   GA  A ++S WG++IAQV+YV+GWC+D W+G S
Sbjct: 242 AWIGFFALTLHIFILYLFINVFKWGLNGAAAAFDVSAWGIAIAQVVYVVGWCKDGWKGLS 301

Query: 246 WLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLNGWENI 305
           WLAF+D+W F+KLSF+SA+M CLEIWY  +IIVL GHL + VI+V SLSICMN+NGWE +
Sbjct: 302 WLAFQDVWPFLKLSFASAVMLCLEIWYFMTIIVLTGHLEDPVIAVGSLSICMNINGWEGM 361

Query: 306 IFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFAVIFTS 365
           +FIGIN A+SVRVSNELG   PRAAKYSV VT+ ESL++G++  ++I   +D FAVIFT 
Sbjct: 362 LFIGINAAISVRVSNELGSGHPRAAKYSVIVTVIESLVIGVVCAIVILITRDDFAVIFTE 421

Query: 366 SVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLPLGIVL 425
           S  ++K VA LAYLLGITM+LNS+QPVISGVA+G GWQA VAYINL CYY FGLPLG +L
Sbjct: 422 SEEMRKAVADLAYLLGITMILNSLQPVISGVAVGGGWQAPVAYINLFCYYAFGLPLGFLL 481

Query: 426 GYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKW 475
           GY    GV+G+W GMI G ++QT++LL ++Y TNWNKEVE+ S RM++W
Sbjct: 482 GYKTSLGVQGIWIGMICGTSLQTLILLYMIYITNWNKEVEQASERMKQW 530

BLAST of CmoCh13G009690 vs. ExPASy Swiss-Prot
Match: Q9SX83 (Protein DETOXIFICATION 33 OS=Arabidopsis thaliana OX=3702 GN=DTX33 PE=2 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 3.1e-132
Identity = 235/446 (52.69%), Postives = 317/446 (71.08%), Query Frame = 0

Query: 32  ETVKTWTISGPVIFQIVCQYGTNSVTNIFVGQLGELELSGVSIAISVIATFAFGFMFGMG 91
           E+ + W ++GP IF  + QY   ++T  F G+LGELEL+ VS+  SVI+  AFG M GMG
Sbjct: 35  ESKRLWELAGPAIFTAISQYSLGALTQTFSGRLGELELAAVSVENSVISGLAFGVMLGMG 94

Query: 92  SATETLCGQAFGAGQIHMLGVYMQRSWVIMFVCALIITPVYVFATPILKLLGQQDDVAEL 151
           SA ETLCGQA+GAGQI M+G+YMQRSWVI+F  AL + PVY++A PIL   G+   +++ 
Sbjct: 95  SALETLCGQAYGAGQIRMMGIYMQRSWVILFTTALFLLPVYIWAPPILSFFGEAPHISKA 154

Query: 152 AGNFSLLILPQLFSFVLAFPTQKFLQAQSKVWALAWIGFGALLAHVFMLWLFIFEFGWST 211
           AG F+L ++PQLF++   FP QKFLQ+Q KV  +AWI    L+ H    WLFI  F W  
Sbjct: 155 AGKFALWMIPQLFAYAANFPIQKFLQSQRKVLVMAWISGVVLVIHAVFSWLFILYFKWGL 214

Query: 212 TGAGLALNISGWGLSIAQVIYVMGWCRD-AWQGFSWLAFKDLWGFVKLSFSSAIMFCLEI 271
            GA + LN S W + I Q++Y++    D AW GFS LAF+DL+GFVKLS +SA+M CLE 
Sbjct: 215 VGAAITLNTSWWLIVIGQLLYILITKSDGAWTGFSMLAFRDLYGFVKLSLASALMLCLEF 274

Query: 272 WYMSSIIVLAGHLPNAVISVDSLSICMNLNGWENIIFIGINVAMSVRVSNELGKARPRAA 331
           WY+  ++V+ G LPN +I VD++SICMN+ GW  +I IG N A+SVRVSNELG      A
Sbjct: 275 WYLMVLVVVTGLLPNPLIPVDAISICMNIEGWTAMISIGFNAAISVRVSNELGAGNAALA 334

Query: 332 KYSVYVTIAESLILGLLFMVLIFFVKDHFAVIFTSSVAVQKYVAKLAYLLGITMVLNSVQ 391
           K+SV V    S ++G++ M+++   KD F  +FTSS AV     ++A LLG T++LNS+Q
Sbjct: 335 KFSVIVVSITSTLIGIVCMIVVLATKDSFPYLFTSSEAVAAETTRIAVLLGFTVLLNSLQ 394

Query: 392 PVISGVAIGAGWQALVAYINLGCYYIFGLPLGIVLGYVAKFGVKGLWGGMIAGIAMQTIL 451
           PV+SGVA+GAGWQALVAY+N+ CYYI GLP G+VLG+    GV+G+WGGM+AGI +QT++
Sbjct: 395 PVLSGVAVGAGWQALVAYVNIACYYIIGLPAGLVLGFTLDLGVQGIWGGMVAGICLQTLI 454

Query: 452 LLLVLYKTNWNKEVEETSGRMQKWTG 477
           L+ ++Y TNWNKE E+   R+Q+W G
Sbjct: 455 LIGIIYFTNWNKEAEQAESRVQRWGG 480

BLAST of CmoCh13G009690 vs. ExPASy Swiss-Prot
Match: Q9LPV4 (Protein DETOXIFICATION 31 OS=Arabidopsis thaliana OX=3702 GN=DTX31 PE=2 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.7e-122
Identity = 221/475 (46.53%), Postives = 316/475 (66.53%), Query Frame = 0

Query: 3   AAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIFVG 62
           A+ L+  SS+  D  P      F R    E+ K W ++GP IF  + QY   +VT +F G
Sbjct: 38  ASSLVSFSSTAVDIPPISGVGDFVREFRIESRKLWKLAGPAIFTTMSQYSLGAVTQVFAG 97

Query: 63  QLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVIMF 122
            +  L L+ VSI  SVIA F+FG M GMGSA ETLCGQAFGAG++ MLGVY+QRSWVI+ 
Sbjct: 98  HISTLALAAVSIENSVIAGFSFGIMLGMGSALETLCGQAFGAGKVSMLGVYLQRSWVILS 157

Query: 123 VCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQSKV 182
           V AL ++ +Y+FA PIL  +GQ   ++ +AG FS+ ++PQ+F++ + FPT KFLQ+QSK+
Sbjct: 158 VTALFLSLIYIFAAPILTFIGQTAAISAMAGIFSIYMIPQIFAYAINFPTAKFLQSQSKI 217

Query: 183 WALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMG-WCRDAW 242
             +A I    L+ H F  WL +    W   G  L LN S W + +AQ++Y+    C +AW
Sbjct: 218 MVMAGISGVVLVIHSFFTWLVMSRLHWGLPGLALVLNTSWWVIVVAQLVYIFNCTCGEAW 277

Query: 243 QGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLNG 302
            GF+W AF +LWGFVKLS +SA M CLEIWY  ++++ AG+L NA +SV +LSICMN+ G
Sbjct: 278 SGFTWEAFHNLWGFVKLSLASAAMLCLEIWYFMALVLFAGYLKNAEVSVAALSICMNILG 337

Query: 303 WENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFAV 362
           W  ++  G N A+SVRVSNELG + PR AK+S+ V +  S  +G+     + F ++ + V
Sbjct: 338 WAAMVAFGTNAAVSVRVSNELGASHPRTAKFSLVVAVILSTAIGMFIAAGLLFFRNEYPV 397

Query: 363 IFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLPL 422
           +F     V+  V +L  +L   +V+N+VQPV+SGVA+GAGWQA+VAY+N+ CYY+FG+P 
Sbjct: 398 LFVEDEEVRNVVRELTPMLAFCIVINNVQPVLSGVAVGAGWQAVVAYVNIACYYLFGVPF 457

Query: 423 GIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTG 477
           G++LG+  ++GV G+W GM+ G  +Q+I+L  ++ KTNW KE      R+++W G
Sbjct: 458 GLLLGFKLEYGVMGIWWGMVTGTFVQSIVLTWMICKTNWEKEASMAEERIKEWGG 512

BLAST of CmoCh13G009690 vs. ExPASy Swiss-Prot
Match: Q9LS19 (Protein DETOXIFICATION 30 OS=Arabidopsis thaliana OX=3702 GN=DTX30 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 6.5e-122
Identity = 219/468 (46.79%), Postives = 314/468 (67.09%), Query Frame = 0

Query: 11  SSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIFVGQLGELELS 70
           SS  D  P      F +    E  K W ++GP IF  + QY   + T +F G +  + L+
Sbjct: 24  SSVEDIPPITTVGGFVKEFNVEVKKLWYLAGPAIFMSITQYSLGAATQVFAGHISTIALA 83

Query: 71  GVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVIMFVCALIITP 130
            VS+  SVIA F+FG M GMGSA ETLCGQAFGAG++ MLGVY+QRSWVI+ V A+I++ 
Sbjct: 84  AVSVENSVIAGFSFGVMLGMGSALETLCGQAFGAGKLSMLGVYLQRSWVILNVTAVILSL 143

Query: 131 VYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQSKVWALAWIGF 190
           +Y+FA PIL  +GQ   ++   G FS+ ++PQ+F++ + +PT KFLQ+QSK+  +A I  
Sbjct: 144 LYIFAAPILAFIGQTPAISSATGIFSIYMIPQIFAYAVNYPTAKFLQSQSKIMVMAAISA 203

Query: 191 GALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVM-GWCRDAWQGFSWLAF 250
            AL+ HV + W  I    W T G  + LN S W + +AQ++Y+  G C +AW GFSW AF
Sbjct: 204 VALVLHVLLTWFVIEGLQWGTAGLAVVLNASWWFIVVAQLVYIFSGTCGEAWSGFSWEAF 263

Query: 251 KDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLNGWENIIFIG 310
            +LW FV+LS +SA+M CLE+WY+ ++I+ AG+L NA ISV +LSICMN+ GW  +I IG
Sbjct: 264 HNLWSFVRLSLASAVMLCLEVWYLMAVILFAGYLKNAEISVAALSICMNILGWTAMIAIG 323

Query: 311 INVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFAVIFTSSVAV 370
           +N A+SVRVSNELG   PR AK+S+ V +  S ++GL   + +   +D +  +F     V
Sbjct: 324 MNAAVSVRVSNELGAKHPRTAKFSLLVAVITSTVIGLAISIALLIFRDKYPSLFVGDEEV 383

Query: 371 QKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLPLGIVLGYVA 430
              V  L  +L +++V+N+VQPV+SGVA+GAGWQA+VAY+N+ CYY+FG+P G++LGY  
Sbjct: 384 IIVVKDLTPILAVSIVINNVQPVLSGVAVGAGWQAVVAYVNIVCYYVFGIPFGLLLGYKL 443

Query: 431 KFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQ 478
            FGV G+W GM+ G  +QTI+L  ++ +TNW+ E     GR+++W G+
Sbjct: 444 NFGVMGIWCGMLTGTVVQTIVLTWMICRTNWDTEAAMAEGRIREWGGE 491

BLAST of CmoCh13G009690 vs. ExPASy TrEMBL
Match: A0A6J1EAT2 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111431468 PE=3 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 6.0e-272
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG
Sbjct: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480

Query: 481 TERRAES 488
           TERRAES
Sbjct: 481 TERRAES 487

BLAST of CmoCh13G009690 vs. ExPASy TrEMBL
Match: A0A6J1KMY8 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111495634 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.5e-270
Identity = 484/487 (99.38%), Postives = 485/487 (99.59%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLLGDSSSGGDYAPARNFEQFK +VWSETVKTWTISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLGDSSSGGDYAPARNFEQFKHVVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLW GMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG
Sbjct: 421 LGIVLGYVAKFGVKGLWSGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480

Query: 481 TERRAES 488
           TERRAES
Sbjct: 481 TERRAES 487

BLAST of CmoCh13G009690 vs. ExPASy TrEMBL
Match: A0A6J1KQR9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111495634 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 6.5e-258
Identity = 463/467 (99.14%), Postives = 464/467 (99.36%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLLGDSSSGGDYAPARNFEQFK +VWSETVKTWTISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLGDSSSGGDYAPARNFEQFKHVVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEET 468
           LGIVLGYVAKFGVKGLW GMIAGIAMQTILLLLVLYKTNWNKEV ET
Sbjct: 421 LGIVLGYVAKFGVKGLWSGMIAGIAMQTILLLLVLYKTNWNKEVGET 467

BLAST of CmoCh13G009690 vs. ExPASy TrEMBL
Match: A0A6J1GT32 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111457185 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 2.8e-253
Identity = 448/487 (91.99%), Postives = 471/487 (96.71%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           M+AAPLLG+  +GGDYAPAR F++FK+IVWSETVKTW ISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MDAAPLLGE-GNGGDYAPARTFKEFKKIVWSETVKTWLISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSG+SIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGISIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           M +CAL+ITPVYVFATPILKLLGQQDDVAELAG+FS+LILPQLFSFV+AFPTQKFLQAQS
Sbjct: 121 MLICALLITPVYVFATPILKLLGQQDDVAELAGSFSVLILPQLFSFVVAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGAL+AHV MLWLFIF+ GW TTGAGLALNISGWG++IAQVIYV GWC DA
Sbjct: 181 KVWALAWIGFGALMAHVLMLWLFIFQLGWGTTGAGLALNISGWGIAIAQVIYVFGWCSDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           W+GFSWLAFKDLWGFV+LSFSSAIMFCLEIWYMSSI+VLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WRGFSWLAFKDLWGFVQLSFSSAIMFCLEIWYMSSIVVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIG+NVAMSVRVSNELGK RPRAA YSVYVTIAESL+LGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGVNVAMSVRVSNELGKGRPRAAMYSVYVTIAESLVLGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINL CYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLACYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLWGGMIAGIAMQT+LLLLVLYKTNWNKEVEETSGRMQKWTGQDI 
Sbjct: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTLLLLLVLYKTNWNKEVEETSGRMQKWTGQDIQ 480

Query: 481 TERRAES 488
            + RAES
Sbjct: 481 RDNRAES 486

BLAST of CmoCh13G009690 vs. ExPASy TrEMBL
Match: A0A6J1JZM3 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111490270 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.1e-252
Identity = 448/487 (91.99%), Postives = 468/487 (96.10%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           M+AAPLLGD   GGDYAPAR +++FK+IVWSETVKTW ISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MDAAPLLGD-GYGGDYAPARTYKEFKQIVWSETVKTWLISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
            GQLGELELSG+SIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  AGQLGELELSGISIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           M VCAL+ITPVYVFATPILKLLGQQDDVAELAG+FS+LILPQLFSFV+AFPTQKFLQAQS
Sbjct: 121 MLVCALLITPVYVFATPILKLLGQQDDVAELAGSFSVLILPQLFSFVVAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGAL+ HV MLWLFIF+ GW TTGAGLALNISGWG++IAQVIYV GWC DA
Sbjct: 181 KVWALAWIGFGALMVHVLMLWLFIFQLGWGTTGAGLALNISGWGIAIAQVIYVFGWCSDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           W+GFSWLAFKDLWGFV+LSFSSAIMFCLEIWYMSSI+VLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WRGFSWLAFKDLWGFVQLSFSSAIMFCLEIWYMSSIVVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIG+NVAMSVRVSNELGK RPRAA YSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGVNVAMSVRVSNELGKGRPRAAMYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINL CYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLACYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLWGGMIAGIAMQT+LLLLVLYKTNWNKEVEETSGRMQKWTGQDI 
Sbjct: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTLLLLLVLYKTNWNKEVEETSGRMQKWTGQDIQ 480

Query: 481 TERRAES 488
            + RAES
Sbjct: 481 RDNRAES 486

BLAST of CmoCh13G009690 vs. NCBI nr
Match: XP_022923883.1 (protein DETOXIFICATION 35-like [Cucurbita moschata] >KAG7019866.1 Protein DETOXIFICATION 35 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 946.0 bits (2444), Expect = 1.2e-271
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG
Sbjct: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480

Query: 481 TERRAES 488
           TERRAES
Sbjct: 481 TERRAES 487

BLAST of CmoCh13G009690 vs. NCBI nr
Match: XP_023001519.1 (protein DETOXIFICATION 35-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 941.4 bits (2432), Expect = 3.1e-270
Identity = 484/487 (99.38%), Postives = 485/487 (99.59%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLLGDSSSGGDYAPARNFEQFK +VWSETVKTWTISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLGDSSSGGDYAPARNFEQFKHVVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLW GMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG
Sbjct: 421 LGIVLGYVAKFGVKGLWSGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480

Query: 481 TERRAES 488
           TERRAES
Sbjct: 481 TERRAES 487

BLAST of CmoCh13G009690 vs. NCBI nr
Match: KAG6584269.1 (Protein DETOXIFICATION 35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 941.0 bits (2431), Expect = 4.0e-270
Identity = 485/487 (99.59%), Postives = 485/487 (99.59%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           M VCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MLVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG
Sbjct: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480

Query: 481 TERRAES 488
           TERRA S
Sbjct: 481 TERRAVS 487

BLAST of CmoCh13G009690 vs. NCBI nr
Match: XP_023519797.1 (protein DETOXIFICATION 35-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 932.9 bits (2410), Expect = 1.1e-267
Identity = 481/487 (98.77%), Postives = 483/487 (99.18%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLL DSSSGGDYAPAR F+QFKRIVWSETVKTWTISG VIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLSDSSSGGDYAPARTFKQFKRIVWSETVKTWTISGAVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAV+KYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVRKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480
           LGIVLGYVAKFGVKGLW GMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG
Sbjct: 421 LGIVLGYVAKFGVKGLWSGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQDIG 480

Query: 481 TERRAES 488
           TERRAES
Sbjct: 481 TERRAES 487

BLAST of CmoCh13G009690 vs. NCBI nr
Match: XP_023001518.1 (protein DETOXIFICATION 35-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 899.4 bits (2323), Expect = 1.3e-257
Identity = 463/467 (99.14%), Postives = 464/467 (99.36%), Query Frame = 0

Query: 1   MEAAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60
           MEAAPLLGDSSSGGDYAPARNFEQFK +VWSETVKTWTISGPVIFQIVCQYGTNSVTNIF
Sbjct: 1   MEAAPLLGDSSSGGDYAPARNFEQFKHVVWSETVKTWTISGPVIFQIVCQYGTNSVTNIF 60

Query: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120
           VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI
Sbjct: 61  VGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVI 120

Query: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180
           MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS
Sbjct: 121 MFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQS 180

Query: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240
           KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA
Sbjct: 181 KVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDA 240

Query: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300
           WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN
Sbjct: 241 WQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLN 300

Query: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360
           GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA
Sbjct: 301 GWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFA 360

Query: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420
           VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP
Sbjct: 361 VIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLP 420

Query: 421 LGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEET 468
           LGIVLGYVAKFGVKGLW GMIAGIAMQTILLLLVLYKTNWNKEV ET
Sbjct: 421 LGIVLGYVAKFGVKGLWSGMIAGIAMQTILLLLVLYKTNWNKEVGET 467

BLAST of CmoCh13G009690 vs. TAIR 10
Match: AT4G25640.1 (detoxifying efflux carrier 35 )

HSP 1 Score: 657.1 bits (1694), Expect = 1.1e-188
Identity = 325/479 (67.85%), Postives = 392/479 (81.84%), Query Frame = 0

Query: 4   APLLGDSSSGG----DYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNI 63
           APLL   + GG    DYAPAR++   KR++ +E+ K W I+ PV F I+CQYG +SVTNI
Sbjct: 5   APLL---THGGEVEEDYAPARSWTDVKRVLSTESAKLWMIAAPVGFNIICQYGVSSVTNI 64

Query: 64  FVGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWV 123
           FVG +GE+ELS VSI++SVI TF+FGF+ GMGSA ETLCGQA+GAGQ++MLGVYMQRSW+
Sbjct: 65  FVGHIGEVELSAVSISLSVIGTFSFGFLLGMGSALETLCGQAYGAGQVNMLGVYMQRSWI 124

Query: 124 IMFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQ 183
           I+FV    + P+Y+FATP+L+LLGQ +++A  AG F+LL +PQLFS    FPT KFLQAQ
Sbjct: 125 ILFVSCFFLLPIYIFATPVLRLLGQAEEIAVPAGQFTLLTIPQLFSLAFNFPTSKFLQAQ 184

Query: 184 SKVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRD 243
           SKV A+AWIGF AL  HV MLWLFI EFGW T GA LA NI+ WG +IAQ++YV+GWC +
Sbjct: 185 SKVVAIAWIGFVALSLHVIMLWLFIIEFGWGTNGAALAFNITNWGTAIAQIVYVIGWCNE 244

Query: 244 AWQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNL 303
            W G SWLAFK++W FV+LS +SA+M CLEIWYM SIIVL G L NAVI+VDSLSICMN+
Sbjct: 245 GWTGLSWLAFKEIWAFVRLSIASAVMLCLEIWYMMSIIVLTGRLDNAVIAVDSLSICMNI 304

Query: 304 NGWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHF 363
           NG E ++FIGIN A+SVRVSNELG  RPRAAKYSVYVT+ +SL++GL+FMV I   +DHF
Sbjct: 305 NGLEAMLFIGINAAISVRVSNELGLGRPRAAKYSVYVTVFQSLLIGLVFMVAIIIARDHF 364

Query: 364 AVIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGL 423
           A+IFTSS  +Q+ V+KLAYLLGITMVLNSVQPV+SGVA+G GWQ LVAYINLGCYYIFGL
Sbjct: 365 AIIFTSSKVLQRAVSKLAYLLGITMVLNSVQPVVSGVAVGGGWQGLVAYINLGCYYIFGL 424

Query: 424 PLGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQD 479
           P G +LGY+A FGV GLW GMIAG A+QT+LLL+VLYKTNWNKEVEET  RM+KW G +
Sbjct: 425 PFGYLLGYIANFGVMGLWSGMIAGTALQTLLLLIVLYKTNWNKEVEETMERMKKWGGSE 480

BLAST of CmoCh13G009690 vs. TAIR 10
Match: AT4G25640.2 (detoxifying efflux carrier 35 )

HSP 1 Score: 657.1 bits (1694), Expect = 1.1e-188
Identity = 325/479 (67.85%), Postives = 392/479 (81.84%), Query Frame = 0

Query: 4   APLLGDSSSGG----DYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNI 63
           APLL   + GG    DYAPAR++   KR++ +E+ K W I+ PV F I+CQYG +SVTNI
Sbjct: 5   APLL---THGGEVEEDYAPARSWTDVKRVLSTESAKLWMIAAPVGFNIICQYGVSSVTNI 64

Query: 64  FVGQLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWV 123
           FVG +GE+ELS VSI++SVI TF+FGF+ GMGSA ETLCGQA+GAGQ++MLGVYMQRSW+
Sbjct: 65  FVGHIGEVELSAVSISLSVIGTFSFGFLLGMGSALETLCGQAYGAGQVNMLGVYMQRSWI 124

Query: 124 IMFVCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQ 183
           I+FV    + P+Y+FATP+L+LLGQ +++A  AG F+LL +PQLFS    FPT KFLQAQ
Sbjct: 125 ILFVSCFFLLPIYIFATPVLRLLGQAEEIAVPAGQFTLLTIPQLFSLAFNFPTSKFLQAQ 184

Query: 184 SKVWALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRD 243
           SKV A+AWIGF AL  HV MLWLFI EFGW T GA LA NI+ WG +IAQ++YV+GWC +
Sbjct: 185 SKVVAIAWIGFVALSLHVIMLWLFIIEFGWGTNGAALAFNITNWGTAIAQIVYVIGWCNE 244

Query: 244 AWQGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNL 303
            W G SWLAFK++W FV+LS +SA+M CLEIWYM SIIVL G L NAVI+VDSLSICMN+
Sbjct: 245 GWTGLSWLAFKEIWAFVRLSIASAVMLCLEIWYMMSIIVLTGRLDNAVIAVDSLSICMNI 304

Query: 304 NGWENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHF 363
           NG E ++FIGIN A+SVRVSNELG  RPRAAKYSVYVT+ +SL++GL+FMV I   +DHF
Sbjct: 305 NGLEAMLFIGINAAISVRVSNELGLGRPRAAKYSVYVTVFQSLLIGLVFMVAIIIARDHF 364

Query: 364 AVIFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGL 423
           A+IFTSS  +Q+ V+KLAYLLGITMVLNSVQPV+SGVA+G GWQ LVAYINLGCYYIFGL
Sbjct: 365 AIIFTSSKVLQRAVSKLAYLLGITMVLNSVQPVVSGVAVGGGWQGLVAYINLGCYYIFGL 424

Query: 424 PLGIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTGQD 479
           P G +LGY+A FGV GLW GMIAG A+QT+LLL+VLYKTNWNKEVEET  RM+KW G +
Sbjct: 425 PFGYLLGYIANFGVMGLWSGMIAGTALQTLLLLIVLYKTNWNKEVEETMERMKKWGGSE 480

BLAST of CmoCh13G009690 vs. TAIR 10
Match: AT4G00350.1 (MATE efflux family protein )

HSP 1 Score: 582.8 bits (1501), Expect = 2.6e-166
Identity = 280/469 (59.70%), Postives = 369/469 (78.68%), Query Frame = 0

Query: 6   LLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIFVGQLG 65
           LLG+++   D+ P ++F   K +   ET K W I+ P+ F I+C YG NS T+IFVG +G
Sbjct: 62  LLGETTGDADFPPIQSFRDAKLVCVVETSKLWEIAAPIAFNILCNYGVNSFTSIFVGHIG 121

Query: 66  ELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVIMFVCA 125
           +LELS V+IA+SV++ F+FGF+ GM SA ETLCGQAFGAGQ+ MLGVYMQRSW+I+   +
Sbjct: 122 DLELSAVAIALSVVSNFSFGFLLGMASALETLCGQAFGAGQMDMLGVYMQRSWLILLGTS 181

Query: 126 LIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQSKVWAL 185
           + + P+Y++ATP+L LLGQ+ ++AE++G F+  I+PQ+F+  + FPTQKFLQ+QSKV  +
Sbjct: 182 VCLLPLYIYATPLLILLGQEPEIAEISGKFTTQIIPQMFALAINFPTQKFLQSQSKVGIM 241

Query: 186 AWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMGWCRDAWQGFS 245
           AWIGF AL  H+F+L+LFI  F W   GA  A ++S WG++IAQV+YV+GWC+D W+G S
Sbjct: 242 AWIGFFALTLHIFILYLFINVFKWGLNGAAAAFDVSAWGIAIAQVVYVVGWCKDGWKGLS 301

Query: 246 WLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLNGWENI 305
           WLAF+D+W F+KLSF+SA+M CLEIWY  +IIVL GHL + VI+V SLSICMN+NGWE +
Sbjct: 302 WLAFQDVWPFLKLSFASAVMLCLEIWYFMTIIVLTGHLEDPVIAVGSLSICMNINGWEGM 361

Query: 306 IFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFAVIFTS 365
           +FIGIN A+SVRVSNELG   PRAAKYSV VT+ ESL++G++  ++I   +D FAVIFT 
Sbjct: 362 LFIGINAAISVRVSNELGSGHPRAAKYSVIVTVIESLVIGVVCAIVILITRDDFAVIFTE 421

Query: 366 SVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLPLGIVL 425
           S  ++K VA LAYLLGITM+LNS+QPVISGVA+G GWQA VAYINL CYY FGLPLG +L
Sbjct: 422 SEEMRKAVADLAYLLGITMILNSLQPVISGVAVGGGWQAPVAYINLFCYYAFGLPLGFLL 481

Query: 426 GYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKW 475
           GY    GV+G+W GMI G ++QT++LL ++Y TNWNKEVE+ S RM++W
Sbjct: 482 GYKTSLGVQGIWIGMICGTSLQTLILLYMIYITNWNKEVEQASERMKQW 530

BLAST of CmoCh13G009690 vs. TAIR 10
Match: AT1G47530.1 (MATE efflux family protein )

HSP 1 Score: 473.4 bits (1217), Expect = 2.2e-133
Identity = 235/446 (52.69%), Postives = 317/446 (71.08%), Query Frame = 0

Query: 32  ETVKTWTISGPVIFQIVCQYGTNSVTNIFVGQLGELELSGVSIAISVIATFAFGFMFGMG 91
           E+ + W ++GP IF  + QY   ++T  F G+LGELEL+ VS+  SVI+  AFG M GMG
Sbjct: 35  ESKRLWELAGPAIFTAISQYSLGALTQTFSGRLGELELAAVSVENSVISGLAFGVMLGMG 94

Query: 92  SATETLCGQAFGAGQIHMLGVYMQRSWVIMFVCALIITPVYVFATPILKLLGQQDDVAEL 151
           SA ETLCGQA+GAGQI M+G+YMQRSWVI+F  AL + PVY++A PIL   G+   +++ 
Sbjct: 95  SALETLCGQAYGAGQIRMMGIYMQRSWVILFTTALFLLPVYIWAPPILSFFGEAPHISKA 154

Query: 152 AGNFSLLILPQLFSFVLAFPTQKFLQAQSKVWALAWIGFGALLAHVFMLWLFIFEFGWST 211
           AG F+L ++PQLF++   FP QKFLQ+Q KV  +AWI    L+ H    WLFI  F W  
Sbjct: 155 AGKFALWMIPQLFAYAANFPIQKFLQSQRKVLVMAWISGVVLVIHAVFSWLFILYFKWGL 214

Query: 212 TGAGLALNISGWGLSIAQVIYVMGWCRD-AWQGFSWLAFKDLWGFVKLSFSSAIMFCLEI 271
            GA + LN S W + I Q++Y++    D AW GFS LAF+DL+GFVKLS +SA+M CLE 
Sbjct: 215 VGAAITLNTSWWLIVIGQLLYILITKSDGAWTGFSMLAFRDLYGFVKLSLASALMLCLEF 274

Query: 272 WYMSSIIVLAGHLPNAVISVDSLSICMNLNGWENIIFIGINVAMSVRVSNELGKARPRAA 331
           WY+  ++V+ G LPN +I VD++SICMN+ GW  +I IG N A+SVRVSNELG      A
Sbjct: 275 WYLMVLVVVTGLLPNPLIPVDAISICMNIEGWTAMISIGFNAAISVRVSNELGAGNAALA 334

Query: 332 KYSVYVTIAESLILGLLFMVLIFFVKDHFAVIFTSSVAVQKYVAKLAYLLGITMVLNSVQ 391
           K+SV V    S ++G++ M+++   KD F  +FTSS AV     ++A LLG T++LNS+Q
Sbjct: 335 KFSVIVVSITSTLIGIVCMIVVLATKDSFPYLFTSSEAVAAETTRIAVLLGFTVLLNSLQ 394

Query: 392 PVISGVAIGAGWQALVAYINLGCYYIFGLPLGIVLGYVAKFGVKGLWGGMIAGIAMQTIL 451
           PV+SGVA+GAGWQALVAY+N+ CYYI GLP G+VLG+    GV+G+WGGM+AGI +QT++
Sbjct: 395 PVLSGVAVGAGWQALVAYVNIACYYIIGLPAGLVLGFTLDLGVQGIWGGMVAGICLQTLI 454

Query: 452 LLLVLYKTNWNKEVEETSGRMQKWTG 477
           L+ ++Y TNWNKE E+   R+Q+W G
Sbjct: 455 LIGIIYFTNWNKEAEQAESRVQRWGG 480

BLAST of CmoCh13G009690 vs. TAIR 10
Match: AT1G12950.1 (root hair specific 2 )

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-123
Identity = 221/475 (46.53%), Postives = 316/475 (66.53%), Query Frame = 0

Query: 3   AAPLLGDSSSGGDYAPARNFEQFKRIVWSETVKTWTISGPVIFQIVCQYGTNSVTNIFVG 62
           A+ L+  SS+  D  P      F R    E+ K W ++GP IF  + QY   +VT +F G
Sbjct: 38  ASSLVSFSSTAVDIPPISGVGDFVREFRIESRKLWKLAGPAIFTTMSQYSLGAVTQVFAG 97

Query: 63  QLGELELSGVSIAISVIATFAFGFMFGMGSATETLCGQAFGAGQIHMLGVYMQRSWVIMF 122
            +  L L+ VSI  SVIA F+FG M GMGSA ETLCGQAFGAG++ MLGVY+QRSWVI+ 
Sbjct: 98  HISTLALAAVSIENSVIAGFSFGIMLGMGSALETLCGQAFGAGKVSMLGVYLQRSWVILS 157

Query: 123 VCALIITPVYVFATPILKLLGQQDDVAELAGNFSLLILPQLFSFVLAFPTQKFLQAQSKV 182
           V AL ++ +Y+FA PIL  +GQ   ++ +AG FS+ ++PQ+F++ + FPT KFLQ+QSK+
Sbjct: 158 VTALFLSLIYIFAAPILTFIGQTAAISAMAGIFSIYMIPQIFAYAINFPTAKFLQSQSKI 217

Query: 183 WALAWIGFGALLAHVFMLWLFIFEFGWSTTGAGLALNISGWGLSIAQVIYVMG-WCRDAW 242
             +A I    L+ H F  WL +    W   G  L LN S W + +AQ++Y+    C +AW
Sbjct: 218 MVMAGISGVVLVIHSFFTWLVMSRLHWGLPGLALVLNTSWWVIVVAQLVYIFNCTCGEAW 277

Query: 243 QGFSWLAFKDLWGFVKLSFSSAIMFCLEIWYMSSIIVLAGHLPNAVISVDSLSICMNLNG 302
            GF+W AF +LWGFVKLS +SA M CLEIWY  ++++ AG+L NA +SV +LSICMN+ G
Sbjct: 278 SGFTWEAFHNLWGFVKLSLASAAMLCLEIWYFMALVLFAGYLKNAEVSVAALSICMNILG 337

Query: 303 WENIIFIGINVAMSVRVSNELGKARPRAAKYSVYVTIAESLILGLLFMVLIFFVKDHFAV 362
           W  ++  G N A+SVRVSNELG + PR AK+S+ V +  S  +G+     + F ++ + V
Sbjct: 338 WAAMVAFGTNAAVSVRVSNELGASHPRTAKFSLVVAVILSTAIGMFIAAGLLFFRNEYPV 397

Query: 363 IFTSSVAVQKYVAKLAYLLGITMVLNSVQPVISGVAIGAGWQALVAYINLGCYYIFGLPL 422
           +F     V+  V +L  +L   +V+N+VQPV+SGVA+GAGWQA+VAY+N+ CYY+FG+P 
Sbjct: 398 LFVEDEEVRNVVRELTPMLAFCIVINNVQPVLSGVAVGAGWQAVVAYVNIACYYLFGVPF 457

Query: 423 GIVLGYVAKFGVKGLWGGMIAGIAMQTILLLLVLYKTNWNKEVEETSGRMQKWTG 477
           G++LG+  ++GV G+W GM+ G  +Q+I+L  ++ KTNW KE      R+++W G
Sbjct: 458 GLLLGFKLEYGVMGIWWGMVTGTFVQSIVLTWMICKTNWEKEASMAEERIKEWGG 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JTB31.5e-18767.85Protein DETOXIFICATION 35 OS=Arabidopsis thaliana OX=3702 GN=DTX35 PE=1 SV=1[more]
F4JH463.6e-16559.70Protein DETOXIFICATION 34 OS=Arabidopsis thaliana OX=3702 GN=DTX34 PE=2 SV=1[more]
Q9SX833.1e-13252.69Protein DETOXIFICATION 33 OS=Arabidopsis thaliana OX=3702 GN=DTX33 PE=2 SV=1[more]
Q9LPV41.7e-12246.53Protein DETOXIFICATION 31 OS=Arabidopsis thaliana OX=3702 GN=DTX31 PE=2 SV=1[more]
Q9LS196.5e-12246.79Protein DETOXIFICATION 30 OS=Arabidopsis thaliana OX=3702 GN=DTX30 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EAT26.0e-272100.00Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111431468 PE=3 SV=1[more]
A0A6J1KMY81.5e-27099.38Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111495634 PE=3 SV=1[more]
A0A6J1KQR96.5e-25899.14Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111495634 PE=3 SV=1[more]
A0A6J1GT322.8e-25391.99Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111457185 PE=3 SV=1[more]
A0A6J1JZM33.1e-25291.99Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111490270 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022923883.11.2e-271100.00protein DETOXIFICATION 35-like [Cucurbita moschata] >KAG7019866.1 Protein DETOXI... [more]
XP_023001519.13.1e-27099.38protein DETOXIFICATION 35-like isoform X2 [Cucurbita maxima][more]
KAG6584269.14.0e-27099.59Protein DETOXIFICATION 35, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023519797.11.1e-26798.77protein DETOXIFICATION 35-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023001518.11.3e-25799.14protein DETOXIFICATION 35-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G25640.11.1e-18867.85detoxifying efflux carrier 35 [more]
AT4G25640.21.1e-18867.85detoxifying efflux carrier 35 [more]
AT4G00350.12.6e-16659.70MATE efflux family protein [more]
AT1G47530.12.2e-13352.69MATE efflux family protein [more]
AT1G12950.11.2e-12346.53root hair specific 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 262..423
e-value: 1.7E-22
score: 79.8
coord: 42..201
e-value: 4.4E-32
score: 111.0
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 42..437
e-value: 9.6E-73
score: 243.3
NoneNo IPR availablePANTHERPTHR11206:SF299PROTEIN DETOXIFICATIONcoord: 12..471
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 12..471
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 32..466
e-value: 4.80498E-156
score: 448.56

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G009690.1CmoCh13G009690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity