CmoCh13G007050 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G007050
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRNase H domain-containing protein
LocationCmo_Chr13: 7165064 .. 7167469 (-)
RNA-Seq ExpressionCmoCh13G007050
SyntenyCmoCh13G007050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCCCCGGAAGGGCCAGCATTGGGGGCGTTCTAAGAGACCATCAGGCTCAGTTTTTGCTGGGATACGCCAAACCCATAGGCCGAGCCAATAGCACCATCGCCGAGCTCACTGCACTCAGCAAAGGGCTCGAATTGGTCCTCGAAAATGGGTGGAAAGACGTATGGCTGGAAGGAGATGCAAAGGGGTTGATAGAAATAATAGTTGGAAAAAAGAAGGCTAAATGTGTGGAAGCTCGAAGCCAATTTCGGTACATAAATTCATTGATATTGGATTTGAATAATTGTAAGGTTAGTCATATATATAGAGAAGGTAATAGAGTTGCCAATAGGTTTGCTTCCATTGGCCATCGCTATAAGAAGCTTGAAGTTTGGAGGGACATTCCTCCATTGGAGACATTGGACATGATGCGTGAAGATGCTGAGGGGAAGATTAGTTTTAGGAACATAAATACAAGGTAGTTAGTTTTGTTTATTTTAAGGTCTTATTTCTGTAACCATTATTTTGAACTTGTAGTTACAAACTGAAAATGATCCGTGATATAATACATGTAATTCTTCAACAATGCATTGATGCACTAACATTATTCGAAATAAATCAACGCCAAATATTAATACATGTGATTCTTCAACCATACATGAACACACATTAATGTATGTCCTAATTCATATATCCAATTATTTATGTTCCCAATATGAATCTAGATTAATGTGGAAGGTTTTGAGACTAGAGTAGTCCGTAAACCTCAATAATCATCGAACGGACTCGCCCAACAATTAGCGACACGCTCCAACCCGTTTCAACTTAACCCAATGGTGCACAACCCAACGGCCCAATCCGCCCACGACCCATTTTATACTGTTTCCTAAGTTTTTGACCCTCCCAAAGTCATTTTTGACCTTATTGAAGCCATTTGAACTCAGTATAACTCCTTTATATTCAAAATGGATATTACCTTATTGAAGCCATTTGAACTCTTTATATTCAAAATTGATATTTTTTTTAGATAAAATTAGAAGTACTGTTATCGTATGTCCTAAAGAATGTGATATGGATTGACATATGTGAAATTAAACATTGTAGCATAGTAGTTTCGAAGTCGGTACAAAATAATCGGGAACTTCAGCCAAGACCAACAAAATAAGTGACTTTACTATTGGAGTTAGATAATTGAATAACGTGGCCCCTGTATGCACCATATTATTTATGTTATGTGTGTGTTCTTTGTAATGACATAAGATGCTATGTTATGTATGCATGTGATATTCATTACGCCATGTTGTAAATGAAATGAAATATTATGTATGTCATGTCTTGCCATGTAAAGATATGGGATGGATCATGTGATGATTGTCGATGTATACATGATGCATATATGTACTCTGTCATGGATGACAGGAGAAACGAAATGAGGTTAATGTTATGCTATGAATGTAAATCATGGGGACCTCATGCATGTATCTATGTCATGTACATCGGAATGCTTCCCCTACGGACATGTACATTTGATATTTACATTTGCCATGATATCATGCGGGTCTTTTCTTTTCAGTGGCGCTCGGTTACGTGTTAACACGCTGTGCTTGATCAAGTCTAAGTTCAGAGGTATGTATACAAGCCTGTCTAGTGCGTCTATATGCGCATGCGTGGACCATTTGGGAAGAAGTATGACACATTTCACCGTGTCAGAGCCCAAGGTCATATTATTATTTTAAAAGATAGGTCTCTTCATGATTGCATTACTATCCCCATACTCGAGCTATATAATTATCCATGTATTATAATTACAATAGTAAGAATGCTCGAATACTCGACACTAAATGCTCGAATGACAAATTGTCATAGAAATCAAAACAGAAGAGTATCCATGTATTATAAGTTCATAATTTGATCTCGAAAATGTTAACTGTGATATCTCTTGTTTAGAGAAGAAAATTAGGGACGTTACAAATATCCACCGACCGGCCGGCGAGCTCGAGTTCTCCCCTATAAGCTCAGATGAACCCATTGTTTGGTAAAGATGACATTACTCCCTGCCCGTTCTAAGTGAAACCCAATAGAAAGCCATCTGAAATTGGGACAAAAAGTTGAAATTGGGAAGGGAAAACAAGGGGGGGTAAGCTTCTAGCACAAAAGACACAGATTAGTACAAAATTCCAAAATTCCTTCAGCGCTGCCATTCACTTAAACGTTTCTTCATCATCCTGAGCTTCTCTTAATCTTGGTTTAACAGAAATTAAAGAGGTCTTACCATTCAAAACGTTGCCCAAATACTACTACTCTGTTTAAACACAACAGAAACAGGGGCCAAAGCCTTTATTATTGTATGCATTCAGGGAACACAGGAAACGCCTAAATTTGCTAAACAAAGAAGTTTGTTGTGTATGGAATTAACACATACATAG

mRNA sequence

ATGTCCCCCGGAAGGGCCAGCATTGGGGGCGTTCTAAGAGACCATCAGGCTCAGTTTTTGCTGGGATACGCCAAACCCATAGGCCGAGCCAATAGCACCATCGCCGAGCTCACTGCACTCAGCAAAGGGCTCGAATTGGTCCTCGAAAATGGGTGGAAAGACGTATGGCTGGAAGGAGATGCAAAGGGGTTGATAGAAATAATAGTTGGAAAAAAGAAGGCTAAATGTGTGGAAGCTCGAAGCCAATTTCGGTACATAAATTCATTGATATTGGATTTGAATAATTGTAAGGTTAGTCATATATATAGAGAAGGTAATAGAGTTGCCAATAGGTTTGCTTCCATTGGCCATCGCTATAAGAAGCTTGAAGTTTGGAGGGACATTCCTCCATTGGAGACATTGGACATGATGCGTGAAGATGCTGAGGGGAAGATTAGTTTTAGGAACATAAATACAAGTGGCGCTCGGTTACGTGTTAACACGCTGTGCTTGATCAAGTCTAAGTTCAGAGAAACAGGGGCCAAAGCCTTTATTATTGTATGCATTCAGGGAACACAGGAAACGCCTAAATTTGCTAAACAAAGAAGTTTGTTGTGTATGGAATTAACACATACATAG

Coding sequence (CDS)

ATGTCCCCCGGAAGGGCCAGCATTGGGGGCGTTCTAAGAGACCATCAGGCTCAGTTTTTGCTGGGATACGCCAAACCCATAGGCCGAGCCAATAGCACCATCGCCGAGCTCACTGCACTCAGCAAAGGGCTCGAATTGGTCCTCGAAAATGGGTGGAAAGACGTATGGCTGGAAGGAGATGCAAAGGGGTTGATAGAAATAATAGTTGGAAAAAAGAAGGCTAAATGTGTGGAAGCTCGAAGCCAATTTCGGTACATAAATTCATTGATATTGGATTTGAATAATTGTAAGGTTAGTCATATATATAGAGAAGGTAATAGAGTTGCCAATAGGTTTGCTTCCATTGGCCATCGCTATAAGAAGCTTGAAGTTTGGAGGGACATTCCTCCATTGGAGACATTGGACATGATGCGTGAAGATGCTGAGGGGAAGATTAGTTTTAGGAACATAAATACAAGTGGCGCTCGGTTACGTGTTAACACGCTGTGCTTGATCAAGTCTAAGTTCAGAGAAACAGGGGCCAAAGCCTTTATTATTGTATGCATTCAGGGAACACAGGAAACGCCTAAATTTGCTAAACAAAGAAGTTTGTTGTGTATGGAATTAACACATACATAG

Protein sequence

MSPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDAKGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKKLEVWRDIPPLETLDMMREDAEGKISFRNINTSGARLRVNTLCLIKSKFRETGAKAFIIVCIQGTQETPKFAKQRSLLCMELTHT
Homology
BLAST of CmoCh13G007050 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 8.5e-07
Identity = 45/152 (29.61%), Postives = 68/152 (44.74%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PG AS GGVLRD    +  G++  IGR ++  AEL  +  GL    E     V LE D+
Sbjct: 471 NPGLASAGGVLRDCTGAWCGGFSLNIGRCSAPQAELWGVYYGLYFAWEKKVPRVELEVDS 530

Query: 62  KGLIEIIVGKKKAKCVEARS---QFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHR 121
               E+IVG  K    ++       R  +  +      ++ H+YRE NR+A+  A+    
Sbjct: 531 ----EVIVGFLKTGISDSHPLSFLVRLCHGFLQKDWLVRIVHVYREANRLADGLANYAFS 590

Query: 122 YKKLEVWRDIPPLETLDMMREDAEGKISFRNI 151
                   D+ P     ++RED  G    R +
Sbjct: 591 LSLGFHSFDLVPDAMSSLLREDTLGSTRPRRV 618

BLAST of CmoCh13G007050 vs. ExPASy TrEMBL
Match: A0A5D3DS33 (Putative ribonuclease H protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00240 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.2e-64
Identity = 119/147 (80.95%), Postives = 135/147 (91.84%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PG ASIGGVLRDH+AQFLLGYA+ IGRANS +AELTAL+KGLELVLENGWKDVW+EGD 
Sbjct: 56  APGLASIGGVLRDHKAQFLLGYAEAIGRANSAMAELTALTKGLELVLENGWKDVWVEGDD 115

Query: 62  KGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKK 121
           KGL+EI+   K+ KCVE RS FRYI SLILDL+NCKVSHIYREGN+VA+RFASIGHRYKK
Sbjct: 116 KGLVEILADNKEVKCVETRSYFRYIKSLILDLDNCKVSHIYREGNKVADRFASIGHRYKK 175

Query: 122 LEVWRDIPPLETLDMMREDAEGKISFR 149
           LE+WR++PPLETLDMMR DAEGKI+FR
Sbjct: 176 LEIWRELPPLETLDMMRHDAEGKITFR 202

BLAST of CmoCh13G007050 vs. ExPASy TrEMBL
Match: A0A0A0LUF5 (RNase H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G071820 PE=4 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 2.4e-60
Identity = 112/148 (75.68%), Postives = 133/148 (89.86%), Query Frame = 0

Query: 1   MSPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGD 60
           + PG ASIGGVLRDH+AQFLLGYA+ IGRA S++AEL AL+KGLELVLENGWKDVW+EGD
Sbjct: 56  IGPGVASIGGVLRDHKAQFLLGYAESIGRAYSSMAELKALTKGLELVLENGWKDVWVEGD 115

Query: 61  AKGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYK 120
           AKGL+EI+   ++ KC+EARS  R+I SL+LD +NCKVSHIYREGN+VA+RFASIGHR K
Sbjct: 116 AKGLVEILAENREVKCMEARSYLRHIKSLLLDFDNCKVSHIYREGNKVADRFASIGHRCK 175

Query: 121 KLEVWRDIPPLETLDMMREDAEGKISFR 149
           KLE+WR++PPLETLDMMR DAEGKI+FR
Sbjct: 176 KLEIWRELPPLETLDMMRHDAEGKITFR 203

BLAST of CmoCh13G007050 vs. ExPASy TrEMBL
Match: A0A314KMR0 (Putative ribonuclease h protein OS=Nicotiana attenuata OX=49451 GN=A4A49_25795 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 6.7e-47
Identity = 84/145 (57.93%), Postives = 118/145 (81.38%), Query Frame = 0

Query: 4   GRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDAKG 63
           G++SIGG+ R+H+A+FLLGYA+ IG+ NSTIAELTALS+GLEL++ENGW DVWLEGD+K 
Sbjct: 56  GKSSIGGIFRNHEAEFLLGYAESIGKTNSTIAELTALSRGLELIIENGWNDVWLEGDSKS 115

Query: 64  LIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKKLE 123
           L+++IV K+  +C E + Q  +IN LI ++ +C ++HI+REGNR A++FA +GH  KK +
Sbjct: 116 LVDVIVSKRVVRCKEVQKQISHINLLIPEIKSCIITHIFREGNRAADKFAQMGHHLKKPQ 175

Query: 124 VWRDIPPLETLDMMREDAEGKISFR 149
           +WRDIPP + L +++EDAEGK  FR
Sbjct: 176 IWRDIPPNQVLRIVKEDAEGKTFFR 200

BLAST of CmoCh13G007050 vs. ExPASy TrEMBL
Match: A0A2P6Q3E3 (Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0005841 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 8.7e-47
Identity = 90/146 (61.64%), Postives = 115/146 (78.77%), Query Frame = 0

Query: 5   RASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDAKGL 64
           +ASIGG+ R+H+A+FLLGYA+ IGRANS IAEL AL +GLELVLENGW DVWLEGDAK L
Sbjct: 63  KASIGGLFRNHKAEFLLGYAESIGRANSVIAELAALRRGLELVLENGWSDVWLEGDAKTL 122

Query: 65  IEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKKLEV 124
           + II  +K+ +C EA+     INS+I +LNNC ++HIYREGNR A++FA +GH Y+K ++
Sbjct: 123 LHIIAKRKQVRCAEAQRHVCEINSIIPELNNCVLTHIYREGNRAADKFAKMGHWYQKPQI 182

Query: 125 WRDIPPLETLDMMREDAEGKISFRNI 151
           W  IPP + L +M EDAEGK+ FR I
Sbjct: 183 WEHIPPHQVLSIMHEDAEGKVLFRKI 208

BLAST of CmoCh13G007050 vs. ExPASy TrEMBL
Match: M5WIY9 (RNase H domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa019260mg PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-46
Identity = 90/147 (61.22%), Postives = 116/147 (78.91%), Query Frame = 0

Query: 4   GRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDAKG 63
           G+ASIGG+ R+H+A FLLGYA+PIGRANS IAEL AL +GLELVLENGW DVWLEGDAK 
Sbjct: 23  GKASIGGLFRNHKADFLLGYAEPIGRANSIIAELAALRRGLELVLENGWSDVWLEGDAKT 82

Query: 64  LIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKKLE 123
           L++II  +++ +C EA+    +IN +I ++NNC ++HIYREGNR A++FA +GH   K +
Sbjct: 83  LLQIIAQRRQIRCAEAQRHISHINLIIPEINNCILTHIYREGNRAADKFAQMGHLCGKPQ 142

Query: 124 VWRDIPPLETLDMMREDAEGKISFRNI 151
           VW+ IPP + L +M EDAEGKI FR I
Sbjct: 143 VWQHIPPNQVLSIMHEDAEGKIVFRKI 169

BLAST of CmoCh13G007050 vs. NCBI nr
Match: KAG6584039.1 (putative ribonuclease H protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 309.3 bits (791), Expect = 2.5e-80
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0

Query: 1   MSPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGD 60
           MSPGRASIGGVLRDHQAQFLLGYA+PIGRANSTIAELTALSKGLELVLENGWKDVWLEGD
Sbjct: 56  MSPGRASIGGVLRDHQAQFLLGYAEPIGRANSTIAELTALSKGLELVLENGWKDVWLEGD 115

Query: 61  AKGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYK 120
           AKGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYK
Sbjct: 116 AKGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYK 175

Query: 121 KLEVWRDIPPLETLDMMREDAEGKISFRNINT 153
           KLEVWRDIPPLETLDMMREDAEGKISFRNINT
Sbjct: 176 KLEVWRDIPPLETLDMMREDAEGKISFRNINT 207

BLAST of CmoCh13G007050 vs. NCBI nr
Match: KAA0056936.1 (putative ribonuclease H protein [Cucumis melo var. makuwa] >TYK26364.1 putative ribonuclease H protein [Cucumis melo var. makuwa])

HSP 1 Score: 256.1 bits (653), Expect = 2.5e-64
Identity = 119/147 (80.95%), Postives = 135/147 (91.84%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PG ASIGGVLRDH+AQFLLGYA+ IGRANS +AELTAL+KGLELVLENGWKDVW+EGD 
Sbjct: 56  APGLASIGGVLRDHKAQFLLGYAEAIGRANSAMAELTALTKGLELVLENGWKDVWVEGDD 115

Query: 62  KGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKK 121
           KGL+EI+   K+ KCVE RS FRYI SLILDL+NCKVSHIYREGN+VA+RFASIGHRYKK
Sbjct: 116 KGLVEILADNKEVKCVETRSYFRYIKSLILDLDNCKVSHIYREGNKVADRFASIGHRYKK 175

Query: 122 LEVWRDIPPLETLDMMREDAEGKISFR 149
           LE+WR++PPLETLDMMR DAEGKI+FR
Sbjct: 176 LEIWRELPPLETLDMMRHDAEGKITFR 202

BLAST of CmoCh13G007050 vs. NCBI nr
Match: KAE8652698.1 (hypothetical protein Csa_013405 [Cucumis sativus])

HSP 1 Score: 241.9 bits (616), Expect = 4.9e-60
Identity = 114/158 (72.15%), Postives = 135/158 (85.44%), Query Frame = 0

Query: 1   MSPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGD 60
           + PG ASIGGVLRDH+AQFLLGYA+ IGRA S++AEL AL+KGLELVLENGWKDVW+EGD
Sbjct: 56  IGPGVASIGGVLRDHKAQFLLGYAESIGRAYSSMAELKALTKGLELVLENGWKDVWVEGD 115

Query: 61  AKGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYK 120
           AKGL+EI+   ++ KC+EARS  R+I SL+LD +NCKVSHIYREGN+VA+RFASIGHR K
Sbjct: 116 AKGLVEILAENREVKCMEARSYLRHIKSLLLDFDNCKVSHIYREGNKVADRFASIGHRCK 175

Query: 121 KLEVWRDIPPLETLDMMREDAEGKISFRNINTSGARLR 159
           KLE+WR++PPLETLDMMR DAEGKI+FR     G   R
Sbjct: 176 KLEIWRELPPLETLDMMRHDAEGKITFRRRCVYGGNSR 213

BLAST of CmoCh13G007050 vs. NCBI nr
Match: XP_038895327.1 (uncharacterized protein LOC120083578 [Benincasa hispida])

HSP 1 Score: 212.2 bits (539), Expect = 4.1e-51
Identity = 106/151 (70.20%), Postives = 117/151 (77.48%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PGRASIGGVLRDH AQFLLGYA+PIGRANST+AELTAL+KGLELVLENGWKDVW+EGDA
Sbjct: 54  TPGRASIGGVLRDHDAQFLLGYAEPIGRANSTMAELTALTKGLELVLENGWKDVWVEGDA 113

Query: 62  KGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKK 121
           KGL+EIIV  K+ KCVEARS F                           RFASIGHRYKK
Sbjct: 114 KGLVEIIVENKEVKCVEARSHF---------------------------RFASIGHRYKK 173

Query: 122 LEVWRDIPPLETLDMMREDAEGKISFRNINT 153
           LE+WR+IPPLE LDMMR+DAEGKI+FR INT
Sbjct: 174 LEIWREIPPLEILDMMRQDAEGKITFRRINT 177

BLAST of CmoCh13G007050 vs. NCBI nr
Match: KAG5516709.1 (hypothetical protein RHGRI_037449 [Rhododendron griersonianum])

HSP 1 Score: 197.2 bits (500), Expect = 1.4e-46
Identity = 89/145 (61.38%), Postives = 115/145 (79.31%), Query Frame = 0

Query: 4   GRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDAKG 63
           G+ASIGGV R+H+A+FLLGYA+ IGR  STIAEL AL +GLELVLENGW+DVWLEGD+K 
Sbjct: 56  GKASIGGVFRNHKAEFLLGYAEAIGRTTSTIAELAALRRGLELVLENGWEDVWLEGDSKA 115

Query: 64  LIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKKLE 123
           L+EII  +K+ KC EA+    +IN +I +LNNC +SH+YREGN+ A++FA +GH+ KK +
Sbjct: 116 LVEIIARRKRVKCAEAQGHISHINLMIPELNNCMLSHVYREGNKAADKFAQMGHQMKKPQ 175

Query: 124 VWRDIPPLETLDMMREDAEGKISFR 149
           VWR  PP + L +M +DAEGKI  R
Sbjct: 176 VWRHNPPHQVLRIMHDDAEGKIFLR 200

BLAST of CmoCh13G007050 vs. TAIR 10
Match: AT3G01410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 5.1e-07
Identity = 32/113 (28.32%), Postives = 52/113 (46.02%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PG+A  G VLR      L    + +G A + +AE  AL  GL   L+ G+K+V + GD+
Sbjct: 166 NPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKGFKNVHVLGDS 225

Query: 62  KGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFAS 115
             +   + G  K    +     +    L+       + HI RE N  A++ A+
Sbjct: 226 MLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADKQAN 278

BLAST of CmoCh13G007050 vs. TAIR 10
Match: AT3G01410.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 5.1e-07
Identity = 32/113 (28.32%), Postives = 52/113 (46.02%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PG+A  G VLR      L    + +G A + +AE  AL  GL   L+ G+K+V + GD+
Sbjct: 166 NPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKGFKNVHVLGDS 225

Query: 62  KGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFAS 115
             +   + G  K    +     +    L+       + HI RE N  A++ A+
Sbjct: 226 MLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADKQAN 278

BLAST of CmoCh13G007050 vs. TAIR 10
Match: AT1G24090.1 (RNase H family protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.6e-05
Identity = 31/123 (25.20%), Postives = 57/123 (46.34%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PG +    VL+      +    + +G A +  AE  AL  GL+  +E G+K++ ++GD+
Sbjct: 228 NPGLSGAAAVLKTEDGSLICRVRQGLGIATNNAAEYHALILGLKYAIEKGYKNIKVKGDS 287

Query: 62  KGLIEIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKK 121
           K +   I G+ K          +    L     + ++SH+ R  N  A+  A++  R  +
Sbjct: 288 KLVCMQIKGQWKVNHEVLAKLHKEAKLLCNKCVSFEISHVLRNLNADADEQANLAVRLPE 347

Query: 122 LEV 125
            EV
Sbjct: 348 GEV 350

BLAST of CmoCh13G007050 vs. TAIR 10
Match: AT5G42905.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 5.3e-04
Identity = 24/64 (37.50%), Postives = 36/64 (56.25%), Query Frame = 0

Query: 2   SPGRASIGGVLRDHQAQFLLGYAKPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDA 61
           +PG AS GGVLRD +  +  G++  IGR ++  AEL  +  GL    E     V LE D+
Sbjct: 159 NPGLASAGGVLRDCEGAWCGGFSLNIGRCSAQHAELWGVYYGLYFAWEKKVPRVELEVDS 218

Query: 62  KGLI 66
           + ++
Sbjct: 219 EAIV 222

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C2F68.5e-0729.61Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A5D3DS331.2e-6480.95Putative ribonuclease H protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0LUF52.4e-6075.68RNase H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G071820 PE=... [more]
A0A314KMR06.7e-4757.93Putative ribonuclease h protein OS=Nicotiana attenuata OX=49451 GN=A4A49_25795 P... [more]
A0A2P6Q3E38.7e-4761.64Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=7464... [more]
M5WIY91.1e-4661.22RNase H domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_... [more]
Match NameE-valueIdentityDescription
KAG6584039.12.5e-8099.34putative ribonuclease H protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAA0056936.12.5e-6480.95putative ribonuclease H protein [Cucumis melo var. makuwa] >TYK26364.1 putative ... [more]
KAE8652698.14.9e-6072.15hypothetical protein Csa_013405 [Cucumis sativus][more]
XP_038895327.14.1e-5170.20uncharacterized protein LOC120083578 [Benincasa hispida][more]
KAG5516709.11.4e-4661.38hypothetical protein RHGRI_037449 [Rhododendron griersonianum][more]
Match NameE-valueIdentityDescription
AT3G01410.15.1e-0728.32Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G01410.25.1e-0728.32Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G24090.11.6e-0525.20RNase H family protein [more]
AT5G42905.15.3e-0437.50Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..117
e-value: 1.3E-18
score: 69.3
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 3..114
e-value: 2.0E-27
score: 95.5
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 1..118
score: 8.635674
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 3..144
NoneNo IPR availablePANTHERPTHR47723:SF3OS06G0683500 PROTEINcoord: 3..144
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 2..113
e-value: 1.2023E-26
score: 96.6144
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 2..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G007050.1CmoCh13G007050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0016740 transferase activity