CmoCh13G006770 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G006770
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationCmo_Chr13: 7039722 .. 7042087 (+)
RNA-Seq ExpressionCmoCh13G006770
SyntenyCmoCh13G006770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCAAGCAATGGCTTCTCTACCACCACCAGCTCTTTCCACCGCCGCATTTCGCCCATCATCCAGCCTTCGAACATCAAAATACCTTCCCATTAACGCTGCACGAATTTTGTGTAGCGCCGCCGATGGCAAAATTGATAGAAGAGACGTTCTTATTGGGATAGGCGCAGGCGGCCTCTACGGAGCTTCGTCCTTCACCAAAGATTCCACCTTTGCCCTTGCCGCTCCAGTTCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACTTGCCACCTGGCGCCGATCCAACCAACTGCTGCCCGCCTCCGACCACTTCGATCATCGACTTCCTGCCACCGGCGCCGGGAAAACTACGCGTCAGGCCAGCTGCCCATTTGGCGGATCAAAGCTACATAGACAAATATAAGAGAGCCGTTGAGCTAATGAAAGGGCTGCCCGAAGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTAATTGGAGATCCCACCTTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAAAATGCCAGCCATGTACGCCGACCAAAACTCGTCCCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACATTCATAGATCTGGATTTCGATGGGGTTGATTCAAACATAGGCGATAATGCGCAGATAAGAAGTAATCTTAGAATTATGTATCGCCAAATGGTTTCCAATAGTCGTACCCCCAAGCTGTTTTTCGGCAGCGCCTATGTAGCCGGAGACGAACCATCACCAGGTAATGTTTTAGTATAACATTGAGCATAAATTTTCGTGATTTTAATTTTTATTTAGCCATTATTTTTCTTTTTCTCAACTTCTACGTTATTCAACCATTTTTTTAAATTATAGATTAATTTTAAATTCATAAAAGTGAAAAAAAAAATTATTTGGTGGAATAATTTTTGGTTGGATAGAATATTGGATTAATTATTCTGAGAACGTTGGATGATGAAAGTCCCACCTCAACTCTTTTAGGGAATGATTATTAGTTTATAAACAAATAATAGGGTTCGTAGGAGGAGTTTTGGGAAAGCTTAGTCATAAGAGCGGAGTTTTGGGAAAGCTTAGTCATAAGAACTTATGTTCAAAGTGGACAATATCATATCATTGTTGAGAGTCATGTTGGTCTAACCGAGTATACACAAAATGATAGGAGGTGGGTCGGTGGAGAACATCCCACACAATACGGTTCACACATGGTGCGGCGACAACAACGAGCCCAACCTAGAGAACATGGGCAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACATAGACCGAATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACAGGACCTACAATACCCTGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAACCTCGGCTACATCTACCAAGACGTGGAACTCCTATGGCTGGAAACCCGCCCCACGCCTCGTCGAGCCCTCAGAGCCCAAAAAAGCGTCGACAGTAATGTCCCCTCCAGGCGCGTGAAACCGGCAAGGATCAAGTTCCCGGTGACTGTGAAGTCGGCGGTCAGCACGGAAGTGAAGAGGGCGAAGAAATCAAGAAGCAAGAAGGAGAAAGAGGAGGAAGAGGAGATTTTGGTGATTAAGGGAATCGAATTCGACCCGAACACGGCCATTAAATTGGATGTTTATATCAACGATGAAGACGACAGCGAAATCAGAGGCGATAATACAGAGTTTGCAGGGAGCTTTGTGAACGTGCCGCATAAACATGGGCACAAGAAGAAGGTGAAGACGGGGCTTAGATTGGGGATAAGTGAAGTGCTTGAGGATTTGGAAGCTGATGATGACGACAGCATCATCGTCACTATTATCCCCAGGCTTGGAGCTGGTCTCGTGTCCATAGCTGAGATCACGATGGAATATGGAGATTAATTTTAAGTTTATTATATTTCTCCCTACGTTCTATGGCAAAGTAATTTTTTTTTCTTTCTTTTTTTTCAAAAGGGTTAGAGACTGGGAAGGGCAGAAGTTATTTTACCTGTTTGTTTAATAATTCCATCTTTATTGTTTCATTTTATGTTTTAAGATTTTTAAATACTTAAAAAAAATTGATTATTGAGAATTGTTTGATTCCAGGAGAAAGGAGAGGACTGTTCAAGATTGTTG

mRNA sequence

ACCAAGCAATGGCTTCTCTACCACCACCAGCTCTTTCCACCGCCGCATTTCGCCCATCATCCAGCCTTCGAACATCAAAATACCTTCCCATTAACGCTGCACGAATTTTGTGTAGCGCCGCCGATGGCAAAATTGATAGAAGAGACGTTCTTATTGGGATAGGCGCAGGCGGCCTCTACGGAGCTTCGTCCTTCACCAAAGATTCCACCTTTGCCCTTGCCGCTCCAGTTCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACTTGCCACCTGGCGCCGATCCAACCAACTGCTGCCCGCCTCCGACCACTTCGATCATCGACTTCCTGCCACCGGCGCCGGGAAAACTACGCGTCAGGCCAGCTGCCCATTTGGCGGATCAAAGCTACATAGACAAATATAAGAGAGCCGTTGAGCTAATGAAAGGGCTGCCCGAAGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTAATTGGAGATCCCACCTTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAAAATGCCAGCCATGTACGCCGACCAAAACTCGTCCCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACATTCATAGATCTGGATTTCGATGGGGTTGATTCAAACATAGGCGATAATGCGCAGATAAGAAGTAATCTTAGAATTATGTATCGCCAAATGGTTTCCAATAGTCGTACCCCCAAGCTGTTTTTCGGCAGCGCCTATGTAGCCGGAGACGAACCATCACCAGGAGGTGGGTCGGTGGAGAACATCCCACACAATACGGTTCACACATGGTGCGGCGACAACAACGAGCCCAACCTAGAGAACATGGGCAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACATAGACCGAATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACAGGACCTACAATACCCTGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAACCTCGGCTACATCTACCAAGACGTGGAACTCCTATGGCTGGAAACCCGCCCCACGCCTCGTCGAGCCCTCAGAGCCCAAAAAAGCGTCGACAGTAATGTCCCCTCCAGGCGCGTGAAACCGGCAAGGATCAAGTTCCCGGTGACTGTGAAGTCGGCGGTCAGCACGGAAGTGAAGAGGGCGAAGAAATCAAGAAGCAAGAAGGAGAAAGAGGAGGAAGAGGAGATTTTGGTGATTAAGGGAATCGAATTCGACCCGAACACGGCCATTAAATTGGATGTTTATATCAACGATGAAGACGACAGCGAAATCAGAGGCGATAATACAGAGTTTGCAGGGAGCTTTGTGAACGTGCCGCATAAACATGGGCACAAGAAGAAGGTGAAGACGGGGCTTAGATTGGGGATAAGTGAAGTGCTTGAGGATTTGGAAGCTGATGATGACGACAGCATCATCGTCACTATTATCCCCAGGCTTGGAGCTGGTCTCGTGTCCATAGCTGAGATCACGATGGAATATGGAGATTAATTTTAAGTTTATTATATTTCTCCCTACGTTCTATGGCAAAGTAATTTTTTTTTCTTTCTTTTTTTTCAAAAGGGTTAGAGACTGGGAAGGGCAGAAGTTATTTTACCTGTTTGTTTAATAATTCCATCTTTATTGTTTCATTTTATGTTTTAAGATTTTTAAATACTTAAAAAAAATTGATTATTGAGAATTGTTTGATTCCAGGAGAAAGGAGAGGACTGTTCAAGATTGTTG

Coding sequence (CDS)

ATGGCTTCTCTACCACCACCAGCTCTTTCCACCGCCGCATTTCGCCCATCATCCAGCCTTCGAACATCAAAATACCTTCCCATTAACGCTGCACGAATTTTGTGTAGCGCCGCCGATGGCAAAATTGATAGAAGAGACGTTCTTATTGGGATAGGCGCAGGCGGCCTCTACGGAGCTTCGTCCTTCACCAAAGATTCCACCTTTGCCCTTGCCGCTCCAGTTCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACTTGCCACCTGGCGCCGATCCAACCAACTGCTGCCCGCCTCCGACCACTTCGATCATCGACTTCCTGCCACCGGCGCCGGGAAAACTACGCGTCAGGCCAGCTGCCCATTTGGCGGATCAAAGCTACATAGACAAATATAAGAGAGCCGTTGAGCTAATGAAAGGGCTGCCCGAAGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTAATTGGAGATCCCACCTTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAAAATGCCAGCCATGTACGCCGACCAAAACTCGTCCCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACATTCATAGATCTGGATTTCGATGGGGTTGATTCAAACATAGGCGATAATGCGCAGATAAGAAGTAATCTTAGAATTATGTATCGCCAAATGGTTTCCAATAGTCGTACCCCCAAGCTGTTTTTCGGCAGCGCCTATGTAGCCGGAGACGAACCATCACCAGGAGGTGGGTCGGTGGAGAACATCCCACACAATACGGTTCACACATGGTGCGGCGACAACAACGAGCCCAACCTAGAGAACATGGGCAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACATAGACCGAATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACAGGACCTACAATACCCTGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAACCTCGGCTACATCTACCAAGACGTGGAACTCCTATGGCTGGAAACCCGCCCCACGCCTCGTCGAGCCCTCAGAGCCCAAAAAAGCGTCGACAGTAATGTCCCCTCCAGGCGCGTGAAACCGGCAAGGATCAAGTTCCCGGTGACTGTGAAGTCGGCGGTCAGCACGGAAGTGAAGAGGGCGAAGAAATCAAGAAGCAAGAAGGAGAAAGAGGAGGAAGAGGAGATTTTGGTGATTAAGGGAATCGAATTCGACCCGAACACGGCCATTAAATTGGATGTTTATATCAACGATGAAGACGACAGCGAAATCAGAGGCGATAATACAGAGTTTGCAGGGAGCTTTGTGAACGTGCCGCATAAACATGGGCACAAGAAGAAGGTGAAGACGGGGCTTAGATTGGGGATAAGTGAAGTGCTTGAGGATTTGGAAGCTGATGATGACGACAGCATCATCGTCACTATTATCCCCAGGCTTGGAGCTGGTCTCGTGTCCATAGCTGAGATCACGATGGAATATGGAGATTAA

Protein sequence

MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGASSFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQKSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEYGD
Homology
BLAST of CmoCh13G006770 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.4e-200
Identity = 346/596 (58.05%), Postives = 434/596 (72.82%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPIN-------------AARILCSA--------AD 60
           M SL PP ++T      ++   S +   N             A ++ C A        A 
Sbjct: 1   MTSLSPPVVTTPTVPNPATKPLSPFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQ 60

Query: 61  GKIDRRDVLIGIGAGGLYGASSFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPP 120
            K+DRR+VL+G+  GGLYG +    D  FA A P+  P++SKCGP DLP GA PTNCCPP
Sbjct: 61  SKLDRRNVLLGL--GGLYGVAGMGTD-PFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPP 120

Query: 121 PTTSIIDFLPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCA 180
           P+T IIDF  PAP KLR+RP AH  DQ+Y DKY +A+ELMK LP+DDPRSF QQA VHCA
Sbjct: 121 PSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCA 180

Query: 181 YCNGGYDQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGG 240
           YC+G YDQVGFP +E+Q+HNSWLFFPFHRYYLYF+EKILG+LI DPTFALPFWN+D+P G
Sbjct: 181 YCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAG 240

Query: 241 MKMPAMYADQNSSLYDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSN 300
           M +PA+YAD  S LYD+LR+ NH PPT +DLD++G + N+     I +NL+IMYRQMVSN
Sbjct: 241 MPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSN 300

Query: 301 SRTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIF 360
           S+  KLFFG+ Y AGDEP PGGGS+E  PH  VH W GDN +PN E+MGNFYSA RDPIF
Sbjct: 301 SKNAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIF 360

Query: 361 YSHHSNIDRMWSVWKTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYI 420
           ++HHSN+DRMWS+WKTLGG+R DL   +WLD+ F+FYNE A+ VRV+VRDCL+TKNLGY+
Sbjct: 361 FAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYV 420

Query: 421 YQDVELLWLETRPTPRRALRAQKSV-------DSNVPSRRVKPARIKFPVTVKSAVSTEV 480
           YQDV++ WL ++PTPRRA  A   V        + V S     A  +FP+++ S +ST V
Sbjct: 421 YQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVV 480

Query: 481 KRAK-KSRSKKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVN 540
           KR K K RSKK KE+EEEILVI+GIEFD + A+K DVY+ND DD     D TEFAGSFV+
Sbjct: 481 KRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKTEFAGSFVS 540

Query: 541 VPHKHGHKKKVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEY 567
           VPH H HKKK+ T LRLG++++LE++EA+DDDS++VT++P+ GA  V I  I +E+
Sbjct: 541 VPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA--VKIGGIKIEF 591

BLAST of CmoCh13G006770 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 4.2e-173
Identity = 306/544 (56.25%), Postives = 392/544 (72.06%), Query Frame = 0

Query: 44  RRDVLIGIGAGGLYGASSFTKDSTFALAAPVQTPNISKCGPP-DLPPGADP--TNCCPPP 103
           RR+VLIG+  GG+YG  +    +  ALA+P+  P++SKC PP DLP G  P   NCCPP 
Sbjct: 70  RRNVLIGL--GGIYGTLA---TNPSALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPY 129

Query: 104 TTSIIDFLPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAY 163
           +T I DF  P+   LRVR AAHL D  +++KYK+A ELMK LP +DPR+F QQAN+HCAY
Sbjct: 130 STKITDFKFPSNQPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAY 189

Query: 164 CNGGYDQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM 223
           C+G Y Q+GFP +++QVH SWLFFPFHR+YLYFYE+ILG LI DPTFALPFWNYDAP GM
Sbjct: 190 CDGAYSQIGFPDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGM 249

Query: 224 KMPAMYADQNSSLYDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNS 283
           ++P +YAD+ S LYD+LRN +H PPT IDL+F  + S+I  N  I++NL IMYRQ+ SN 
Sbjct: 250 QLPTIYADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNG 309

Query: 284 RTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFY 343
           +T +LF G+ Y AGD    G GS+EN+PH  VHTW GDN + N+E+MG FYSAARDPIFY
Sbjct: 310 KTSRLFLGNPYRAGDAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFY 369

Query: 344 SHHSNIDRMWSVWKTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIY 403
           SHHSN+DR+W +WKTLGG++ D    +WL++ F+FY+E    VRV V+D LD   LGY Y
Sbjct: 370 SHHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAY 429

Query: 404 QDVELLWLETRPTPRRALRAQKSVDSNVPS------------RRVKPAR-IKFPVTVKSA 463
           QDV + W + +P PRR  + QK V+  V              R+  P + + FP+ + + 
Sbjct: 430 QDVPIPWEKAKPVPRRT-KVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNK 489

Query: 464 VSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAG 523
           VS  VKR KK RSKKEKEEEEE+LVI+GIEF  N AIK DVYINDEDD ++   NTEFAG
Sbjct: 490 VSAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYINDEDD-KVGAGNTEFAG 549

Query: 524 SFVNVPHK-HGHK-KKVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITM 569
           SFVN+PH  HGHK KK+ T LRLGI+++LEDL  + DD+I+VT++P+ G+G V I  + +
Sbjct: 550 SFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 606

BLAST of CmoCh13G006770 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.8e-169
Identity = 309/587 (52.64%), Postives = 393/587 (66.95%), Query Frame = 0

Query: 5   PPPALSTAAFRPSSSLRTSKYLPINAARILCSAAD----------------GKIDRRDVL 64
           P P    A+  P +  R+ ++ P   +++ C++A+                GK+DRR+VL
Sbjct: 25  PSPLFQRASHVPVARNRSRRFAP---SKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVL 84

Query: 65  IGIGAGGLYGAS-SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDF 124
           +GI  GGLYGA+         A  AP+Q P+ISKCG   +P G  PTNCCPP TT IIDF
Sbjct: 85  LGI--GGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDF 144

Query: 125 -LPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYD 184
            LP +   +R RPAAHL  + Y+ KYK+A+EL K LP+DDPRSF QQANVHC YC G YD
Sbjct: 145 QLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYD 204

Query: 185 QVGF-PVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMY 244
           QVG+  +E+QVH SWLF PFHRYYLYF E+IL +LI DPTFALP+W +D P GM MP +Y
Sbjct: 205 QVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIY 264

Query: 245 ADQNSSLYDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLF 304
           A   SSLYD+ RN  HLPPT IDLD+DG +  I D+     NL IMY+Q+VS + TPKLF
Sbjct: 265 ASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLF 324

Query: 305 FGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNI 364
            G  Y AGD   PG G++E+ PHN VH W G  ++P+ E+MGNFY+A RDPIF+ HH+N+
Sbjct: 325 LGYPYRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPS-EDMGNFYTAGRDPIFFGHHANV 384

Query: 365 DRMWSVWKTLGGR-RQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL 424
           DRMW++WKT+GG+ R+D    +WLDA+F+FY+E  Q V+V+V DC+DT  L Y YQD+ +
Sbjct: 385 DRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPI 444

Query: 425 LWLETRPTPRRALRAQKSVDSNVPSRRVKPARI-----KFPVTVKSAVSTEVKRAKKSRS 484
            WL  + T  +A    KS  S V      P         FP  + S +  EV R KKSRS
Sbjct: 445 PWL-PKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRS 504

Query: 485 KKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKK 544
           KKEKE+EEE+L+IKGIE D    +K DVYINDED S  R  N+EFAGSFVNVPHKH  + 
Sbjct: 505 KKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRPKNSEFAGSFVNVPHKHMKEM 564

Query: 545 KVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEY 567
           K KT LR  I+E+LEDL A+DD+S+IVTI+PR G   V+I  I +E+
Sbjct: 565 KTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIEF 604

BLAST of CmoCh13G006770 vs. ExPASy Swiss-Prot
Match: Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 5.3e-160
Identity = 291/525 (55.43%), Postives = 371/525 (70.67%), Query Frame = 0

Query: 55  GLYGASS-----FTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDF-L 114
           GLY A+S     F   ST     P+  P+++KCG PDLPPG  P NCCPP    IIDF L
Sbjct: 43  GLYIANSLAYARFASTST----GPIAAPDVTKCGQPDLPPGTAPINCCPPIPAKIIDFEL 102

Query: 115 PPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQV 174
           PP    +RVR AAHL D +YI K+K+AVELM+ LPEDDPRSF QQANVHCAYC G Y+Q 
Sbjct: 103 PPPSTTMRVRRAAHLVDDAYIAKFKKAVELMRALPEDDPRSFKQQANVHCAYCAGAYNQA 162

Query: 175 GFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYAD 234
           GF  +++Q+H SWLFFPFHRYY+YF+E+ILG+LI D TFALPFWNYD+PGGM +P+M+ D
Sbjct: 163 GFTNLKLQIHRSWLFFPFHRYYIYFFERILGKLINDTTFALPFWNYDSPGGMTIPSMFID 222

Query: 235 QNSSLYDQLRNQNHLPPTFIDLD--FDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLF 294
            NSSLYD LR+ NH PPT +DL+  F   D+      Q+  NL+I+YRQMVS+++TP+LF
Sbjct: 223 TNSSLYDSLRDSNHQPPTIVDLNYAFSDSDNTTTPEEQMIINLKIVYRQMVSSAKTPQLF 282

Query: 295 FGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNI 354
           FG  Y  GD+  PG GS+E +PH  +H W G  N P  ENMG FYS ARDPIF++HHSN+
Sbjct: 283 FGRPYRRGDQEFPGVGSIELVPHGMIHLWTGSENTPYGENMGAFYSTARDPIFFAHHSNV 342

Query: 355 DRMWSVWKTLGG-RRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL 414
           DRMWS+WKTLGG RR DL  P++LDASF+FY+E A+ VRV+VRDCLD K LGY+YQDVE+
Sbjct: 343 DRMWSIWKTLGGPRRTDLTDPDFLDASFVFYDENAEMVRVKVRDCLDEKKLGYVYQDVEI 402

Query: 415 LWLETRPTPRRALRAQKSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKE 474
            WL TRPTP+ +    K       +    P ++ FP  +   +   V R KK+RS+KEK+
Sbjct: 403 PWLNTRPTPKVSPSLLKKFHR---TNTANPRQV-FPAILDRVLKVIVTRPKKTRSRKEKD 462

Query: 475 EEEEILVIKGIEFDPNTA-IKLDVYIN-DEDD-SEIRGDNTEFAGSFVNVPHKHGHKKKV 534
           E EEILVI+GIE + +   +K DVYIN DEDD + I  +N EFAGSFV++ HK    K+ 
Sbjct: 463 ELEEILVIEGIELERDHGHVKFDVYINADEDDLAVISPENAEFAGSFVSLWHKPIKGKRT 522

Query: 535 KTG-LRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITME 566
           KT  L L I ++LEDL+AD+DD ++VT++PR     + I  + +E
Sbjct: 523 KTQLLTLSICDILEDLDADEDDYVLVTLVPRNAGDAIKIHNVKIE 559

BLAST of CmoCh13G006770 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 3.5e-159
Identity = 287/536 (53.54%), Postives = 373/536 (69.59%), Query Frame = 0

Query: 42  IDRRDVLIGIGAGGLYGASSFTKDSTFALAAPVQTPNI-SKCGPP-DLPPGADPTNCCPP 101
           IDRR++LIG+  GGLYGAS+    +   LAAP+Q P +   C PP DL  G +   CCPP
Sbjct: 52  IDRRNMLIGL--GGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVN-VLCCPP 111

Query: 102 PTTSIIDF-LPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHC 161
                IDF +P  P +LR+RPAAHLAD +YI+KYK+A+  MK LP++DPRSF QQAN+HC
Sbjct: 112 DVKDPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHC 171

Query: 162 AYCNGGYDQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPG 221
           AYCNG YDQVGFP V +QVH+SWLF PFHR+YLYFYE+ILG LI DPTFA+PFWN+DAP 
Sbjct: 172 AYCNGAYDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPK 231

Query: 222 GMKMPAMYADQNSSLYDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVS 281
           GM MP M+ D NS LYD  RN  H P T +DLDF   ++   +  QI +NL IMY+Q+V 
Sbjct: 232 GMHMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQVV- 291

Query: 282 NSRTPKLFFGSAYVAGDEPSPGG-GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDP 341
            ++  +LF G    AG  P   G GS+E  PH  +H W GD  + N ENMGNFYSA RDP
Sbjct: 292 RAKKARLFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDP 351

Query: 342 IFYSHHSNIDRMWSVWKTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLG 401
           +FY+HH+N+DRMW +WK   GR+ D +  +WL+A F+ ++E AQ VRV VRD LD + LG
Sbjct: 352 LFYAHHANVDRMWYIWKNSLGRK-DYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLG 411

Query: 402 YIYQDVELLWLETRPTPRRALRAQKSVDSNVPSRRVKPARI--KFPVTVKSAVSTEVKRA 461
           Y YQ+V++ W+ ++P PR+A        + + S+     ++  KFP+T+ S VS EVKR 
Sbjct: 412 YAYQEVDIPWINSKPKPRKA----NPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRP 471

Query: 462 KKSRSKKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHK 521
           KKSRSK EKE+EEE+LVI+ I+ +P   +K DVYINDED+     D TEFAGSFVNVPH 
Sbjct: 472 KKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDEDPSAADQTEFAGSFVNVPHF 531

Query: 522 HGH----KKKVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEY 567
           H H     K+  T L +GISEVL++L+ D DDSI+VT++PR+G+G ++I    +E+
Sbjct: 532 HRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIEF 578

BLAST of CmoCh13G006770 vs. ExPASy TrEMBL
Match: A0A6J1EGP5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434015 PE=3 SV=1)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA
Sbjct: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
           TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD
Sbjct: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVTIIPRLGAGLVSIAEITMEYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 568

BLAST of CmoCh13G006770 vs. ExPASy TrEMBL
Match: A0A6J1KRC0 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495765 PE=3 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 530/568 (93.31%), Postives = 551/568 (97.01%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           MASLPPPALSTA FRPSS   TSKYLP NAAR+LCSAA+GKIDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           +FTKDSTFALAAPVQTPNISKCGPPDLPPG+DPTNCCPPP+ SI+DF PPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT +DLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL WLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP ++K PVT+KSA+STEVKR KKSR +KEK+EEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
           T+IKLD+YINDEDD EIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDL+ADD
Sbjct: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVTIIPRLGAGLVSIAEI +EYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 568

BLAST of CmoCh13G006770 vs. ExPASy TrEMBL
Match: A0A6J1KJD3 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495764 PE=3 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.2e-300
Identity = 503/568 (88.56%), Postives = 533/568 (93.84%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           M SL  PALST  FRPSS   TSKYLP NAAR+LCSAADGKIDRRDVLIGIGAGG+YGAS
Sbjct: 1   MGSLSSPALSTTTFRPSSRPPTSKYLPNNAARVLCSAADGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           +FTKDSTFALAAPVQTPNISKC PPDLP GADPTNCCPPP+ SI+DF PP PGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCSPPDLPHGADPTNCCPPPSPSIVDFRPPVPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLP DDPRSFMQQAN+HCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANIHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT +DLDFDGVD  IGD+A I+SNLRIMYRQMVSNS TPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL WLETRPTP RALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPCRALRAQ 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP ++KFPVT+KSA+STEVKR KKSRSKKEKEEEEEILVI+GI+ DPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKFPVTLKSAISTEVKRGKKSRSKKEKEEEEEILVIEGIKLDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
             +K D+YINDED+ E R D +EFAGSFVNVPHKHGHKKK+KTGLRLGI+++LEDL+AD 
Sbjct: 481 MLVKFDIYINDEDEKENRADISEFAGSFVNVPHKHGHKKKMKTGLRLGITQLLEDLDADC 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVT+IPRLGAGLVSIA+I +EYGD
Sbjct: 541 DDSIIVTLIPRLGAGLVSIAKIKIEYGD 568

BLAST of CmoCh13G006770 vs. ExPASy TrEMBL
Match: A0A6J1EMV7 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434014 PE=3 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 1.0e-283
Identity = 483/568 (85.04%), Postives = 511/568 (89.96%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           MASL PPALST  FRPSSSLRTSKYLP NAARILCSAADG IDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           +      FAL APVQTPNISKCGPPDLPP ADPTNCCPPP+ SI+DF PPAPGKLRVRPA
Sbjct: 61  A------FALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHL DQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPT ALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT IDLDFDGVD  IGD+A I+SNLRIMYRQMVSNS TPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRRQ
Sbjct: 301 GSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQ PEWL+ASFIFYNEKAQAVRVRVRDCLDTK LGY+YQDVEL WL+TRP PR A +A 
Sbjct: 361 DLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKA- 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
                       +  ++KFPVT+KSA+STEVKRAKKSRSKKEKEEEEEILVI+GI+ DPN
Sbjct: 421 -----------ARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
             +K D+YINDED+ E R D TEFAGSFVNVPHKHGH KKVKTGLRLGI+E+LEDL AD 
Sbjct: 481 MLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADG 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVT++PRLGAGLVSIA+I +EYGD
Sbjct: 541 DDSIIVTLVPRLGAGLVSIAKIKIEYGD 550

BLAST of CmoCh13G006770 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.9e-259
Identity = 443/590 (75.08%), Postives = 499/590 (84.58%), Query Frame = 0

Query: 6   PPALSTAAF------------RPSSSLRTSKYLPINAARILCSAADG---KIDRRDVLIG 65
           PPALS+AA              P  +LR +KY    A RILCSA++G    IDRRD+LIG
Sbjct: 5   PPALSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQNHIDRRDILIG 64

Query: 66  IGAGGLYGASSFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPP 125
           +  GGLYGAS+ ++ S  A AAPVQTPNISKCG PDLPPGADP NCCPPPTT+++DF P 
Sbjct: 65  L--GGLYGASTLSQGS-LAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFRPQ 124

Query: 126 APGKLRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGF 185
           APGKLRVRPAAHLADQ+Y++KYKRAVELMK LP+DDPRSF QQANVHCAYCNG YDQVGF
Sbjct: 125 APGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQVGF 184

Query: 186 PVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNS 245
           PVEVQVHNSWLFFPFHRYYL+F+EKILGELIGDP+FALPFWNYDAPGGMKMPAMYAD+ S
Sbjct: 185 PVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADEKS 244

Query: 246 SLYDQLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAY 305
           SLYD+LRN+NHLPPT +DLDF GVD  IGD AQIRSNL IMYRQMVSNSRTP LFFG+AY
Sbjct: 245 SLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAY 304

Query: 306 VAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWS 365
           +AGDEPSPGGG VENIPH  VH WCGDNNEPN ENMGNFYSAARDPIFY+HHSNIDR+WS
Sbjct: 305 LAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDRLWS 364

Query: 366 VWKTLGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETR 425
           VWKTLGGRRQDLQ  +WL+ASFIFY+EKAQAVRVRVRDCLD++NLGY+YQDV+L WLE+R
Sbjct: 365 VWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESR 424

Query: 426 PTPRRALRAQKSV--------------DSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKK 485
           PTPRRALRAQK+V              +    S R++PA+IKFP+T+ S +S EVKR K+
Sbjct: 425 PTPRRALRAQKAVAHLPFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISMEVKRPKR 484

Query: 486 SRSKKEKEEEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHG 545
           SRSKKEK+EEEEI+VI GIEFD N AIK DV+INDEDD EIR DNTEFAGSFVNVPHKHG
Sbjct: 485 SRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHKHG 544

Query: 546 HKKKVKTGLRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEY 567
           HKK VKTGLRLGI+E+LEDL+ADDDDS+ VT++PR G GLVSIA I +E+
Sbjct: 545 HKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEF 591

BLAST of CmoCh13G006770 vs. NCBI nr
Match: XP_022927081.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA
Sbjct: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
           TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD
Sbjct: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVTIIPRLGAGLVSIAEITMEYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 568

BLAST of CmoCh13G006770 vs. NCBI nr
Match: KAG6584007.1 (hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 563/568 (99.12%), Postives = 565/568 (99.47%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           +FTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELI DPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIEDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTK LGYIYQDVEL WLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKTLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
           TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD
Sbjct: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVTIIPRLGAGLVSIAEIT+EYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEITIEYGD 568

BLAST of CmoCh13G006770 vs. NCBI nr
Match: XP_023519283.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 548/568 (96.48%), Postives = 561/568 (98.77%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           +FTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSI+DFLPPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIVDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT IDLDFDGVDSNIG+NAQIR+NLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLIDLDFDGVDSNIGNNAQIRTNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEK +AVRVRVRDCLDTKNLGYIYQDVEL WLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKGEAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP R+KFPVTV+SAVSTEVKR KKSRS+KEK+EEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPGRMKFPVTVESAVSTEVKRGKKSRSRKEKDEEEEILVIKGIEFDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
           TAIKLDVYINDED+ EIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGI+EVLEDLEADD
Sbjct: 481 TAIKLDVYINDEDEREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGITEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVTIIP LGAGLVSIA+IT+EYGD
Sbjct: 541 DDSIIVTIIPSLGAGLVSIADITLEYGD 568

BLAST of CmoCh13G006770 vs. NCBI nr
Match: XP_023001713.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 530/568 (93.31%), Postives = 551/568 (97.01%), Query Frame = 0

Query: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
           MASLPPPALSTA FRPSS   TSKYLP NAAR+LCSAA+GKIDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
           +FTKDSTFALAAPVQTPNISKCGPPDLPPG+DPTNCCPPP+ SI+DF PPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT +DLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL WLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP ++K PVT+KSA+STEVKR KKSR +KEK+EEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480

Query: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
           T+IKLD+YINDEDD EIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDL+ADD
Sbjct: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
           DDSIIVTIIPRLGAGLVSIAEI +EYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 568

BLAST of CmoCh13G006770 vs. NCBI nr
Match: KAG7019629.1 (hypothetical protein SDJN02_18592, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 538/568 (94.72%), Postives = 541/568 (95.25%), Query Frame = 0

Query: 1    MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60
            MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS
Sbjct: 503  MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 562

Query: 61   SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120
            +FTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA
Sbjct: 563  AFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 622

Query: 121  AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
            AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 623  AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 682

Query: 181  LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240
            LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN
Sbjct: 683  LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 742

Query: 241  HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
            HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSP  
Sbjct: 743  HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSP-- 802

Query: 301  GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360
                                   ENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ
Sbjct: 803  -----------------------ENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 862

Query: 361  DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420
            DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTK LGYIYQDV+L WLETRPTPRRALRAQ
Sbjct: 863  DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKTLGYIYQDVDLPWLETRPTPRRALRAQ 922

Query: 421  KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480
            KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN
Sbjct: 923  KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 982

Query: 481  TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540
            TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD
Sbjct: 983  TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 1042

Query: 541  DDSIIVTIIPRLGAGLVSIAEITMEYGD 569
            DDSIIVTIIPRLGAGLVSIAEIT+EYGD
Sbjct: 1043 DDSIIVTIIPRLGAGLVSIAEITIEYGD 1045

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P433091.4e-20058.05Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q062154.2e-17356.25Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
P433114.8e-16952.64Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q9FRX65.3e-16055.43Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1[more]
Q6UIL33.5e-15953.54(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1EGP50.0e+00100.00polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KRC00.0e+0093.31polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A6J1KJD31.2e-30088.56polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A6J1EMV71.0e-28385.04polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A1L5JHU38.9e-25975.08Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022927081.10.0e+00100.00polyphenol oxidase, chloroplastic-like [Cucurbita moschata][more]
KAG6584007.10.0e+0099.12hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023519283.10.0e+0096.48polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023001713.10.0e+0093.31polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
KAG7019629.10.0e+0094.72hypothetical protein SDJN02_18592, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 177..194
score: 58.96
coord: 334..352
score: 54.78
coord: 208..213
score: 55.1
coord: 307..318
score: 41.33
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 147..354
e-value: 1.1E-33
score: 117.5
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 177..194
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 335..346
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 29..568
e-value: 6.0E-248
score: 822.4
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 72..408
e-value: 2.4E-121
score: 407.3
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 77..406
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 361..412
e-value: 4.1E-21
score: 74.5
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 439..566
e-value: 1.4E-47
score: 161.0
NoneNo IPR availablePIRSRPIRSR000290-2PIRSR000290-2coord: 72..567
e-value: 3.5E-230
score: 762.9
NoneNo IPR availablePIRSRPIRSR000290-1PIRSR000290-1coord: 72..567
e-value: 3.5E-230
score: 762.9
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 23..566
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 23..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G006770.1CmoCh13G006770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity