CmoCh13G005180 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G005180
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr13: 6084982 .. 6087165 (-)
RNA-Seq ExpressionCmoCh13G005180
SyntenyCmoCh13G005180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTCCTTAGACCTTATGGTAAGGTCGATGAATCCGATCAAATGAGGCTCGACGCTAGCCGAAGGACTCGGAAGAGGGTCATTGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTTGTGTTCGGGACTAGAAATAGTTCCAAGAACGACGAGAGTGACAATCACAATGAGGCTCTTTCAACTTCGATAAAAGCAGTTTGTGATGAGACTTTGTATCCCGATACTTGTAAGAATGCTTTTGGTTCTCTAGCTAACTCGTCCCACCTCGACCCTGGTCAAATAGCCAAGTTTTCGGTTCAACTCGCGCTCGGTGAACTGTCGCGAGTGGCTGATTACCTTTTGGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTAGCTTTGAGAAGTTGTCATGAACTTTTGGACTTGGCAATAGATCATCTAAATGACTCATTATCATCAAGTGAGATTACAGTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAAGTTCAGCAGCTACTTACCAGCAAACTTGCATCGACGGGCTCGAGGAAGTCGATCTCGCTTTGACGAATGTCATCGCAAATTACTTGAAGAACTCTACTGAAATGACCAGCAATGGCCTTGCCATTGTGTCCTTCTTTTCGAAGCTAACGGATTCTTTCAGTCTACGACGATTGATGAGTTATGAAGATCGACATAGCAATGGAGATTGGGCGCGCCCGATACTCCGGAAGCTAGCTCAAAAGGATCTCCGGAAGCAGGCCGACATCGTCGTAGCGAAAGACAAATCGGGGAAGTACAAAACAATCACTGAAGCTCTCAATGCTGTACCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTGAAGAAGGGAGTTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTAGTGATGATTGGCGATGGAATGACTTCAACCACTGTAACTGGCAGGCTCAACTTCATAGATGGGACGCCAACATTTTCTACTGCAACATTTGGTAACATTTTCTAAGCTTACGAGTCCCACGTTGGTTAATTTAGGGAATGATTATGTGTTTATAAGTAAGGAATACATCTTTATTGGTACAAGGTCTTTTGGGGAAGCCCAAAGTAAAGCCACGAGAGCTTATGCTTAAAGTGGACAATATCATACCTTTGTGGAGGGTCGTGATTCTTAACATGGTATTAGAGCTATGCCCTGAACTTAACCATGTCAATAGAATCCTCAAATGTCGAACAAAGGGTGTACTTTGTTCGAGGACTCTAGAAAAGGAGTCGAGCCTTGATTAAGGGGAGATTGTTTGATGGGCACATAGACCTTAGGGGAGGCTCTATGGTGTACTTTGTTCGAGGGGAGCATTGGTACGAGGTAAACCTAAAGTAAAGCTAGAGCTTAGGTTCAAAGTGGACAATATCATACTAGTGTGGAGAGTCGTGATTCCTAGCATGCTTAGAATCTAACAGTGTGTTTTTTTGTGCAGCTGCAAAGGGAAAAGGCTTCATTGCCATAGACATGGGATTTGAGAACACAGCTGGACCGAGCAAACACCAGGCGGTTGCTCTGATGTCGACGTCGGACCAATCGATCTTCTACCGCTGCCAAATGAATGCATACCAAGACACTCTCTATGCACACTCGAACCGTCAATTCTACCGTGACTGCAAGGTTTACGGCACGGTCGATTTCATCTTTGGAAACTCAGCAGTAGTCCTACAAAACTGCAAAATTGCACCAAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCCGGTTTGATCCTAATCAACACACTGGCATTTCAATACAACACTCTTCAATCCAACCGTTCGAAAACTTGAAAACTACCGAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACGACCGTGTTTATGCAGAACGATTTCGGTAGCTTGATTCACCCAACTGGCTGGCTGCCATGGGTTGGAACATCAGCACCAGACACCATCTTCTATGCTGAGTTTCAAAACAAAGGCCCTGGTTCTTCCACAGCCAACAGAGTGAAATGGAAAGGTTTGAAGAATATTGACACCAAAACTGCCAAGAAATTCACTGTTTCCTCTTTCATTCACGGTAAAGATTGGATCTCAAAGGCTCAAGTCCCCTTCAATGCCACCCTCTGA

mRNA sequence

ATGTCTCTCCTTAGACCTTATGGTAAGGTCGATGAATCCGATCAAATGAGGCTCGACGCTAGCCGAAGGACTCGGAAGAGGGTCATTGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTTGTGTTCGGGACTAGAAATAGTTCCAAGAACGACGAGAGTGACAATCACAATGAGGCTCTTTCAACTTCGATAAAAGCAGTTTGTGATGAGACTTTGTATCCCGATACTTGTAAGAATGCTTTTGGTTCTCTAGCTAACTCGTCCCACCTCGACCCTGGTCAAATAGCCAAGTTTTCGGTTCAACTCGCGCTCGGTGAACTGTCGCGAGTGGCTGATTACCTTTTGGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTAGCTTTGAGAAGTTGTCATGAACTTTTGGACTTGGCAATAGATCATCTAAATGACTCATTATCATCAAGTGAGATTACAGTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAAGTTCAGCAGCTACTTACCAGCAAACTTGCATCGACGGGCTCGAGGAAGTCGATCTCGCTTTGACGAATGTCATCGCAAATTACTTGAAGAACTCTACTGAAATGACCAGCAATGGCCTTGCCATTGTGTCCTTCTTTTCGAAGCTAACGGATTCTTTCAGTCTACGACGATTGATGAGTTATGAAGATCGACATAGCAATGGAGATTGGGCGCGCCCGATACTCCGGAAGCTAGCTCAAAAGGATCTCCGGAAGCAGGCCGACATCGTCGTAGCGAAAGACAAATCGGGGAAGTACAAAACAATCACTGAAGCTCTCAATGCTGTACCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTGAAGAAGGGAGTTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTAGTGATGATTGGCGATGGAATGACTTCAACCACTGTAACTGGCAGGCTCAACTTCATAGATGGGACGCCAACATTTTCTACTGCAACATTTGCTGCAAAGGGAAAAGGCTTCATTGCCATAGACATGGGATTTGAGAACACAGCTGGACCGAGCAAACACCAGGCGGTTGCTCTGATGTCGACGTCGGACCAATCGATCTTCTACCGCTGCCAAATGAATGCATACCAAGACACTCTCTATGCACACTCGAACCGTCAATTCTACCGTGACTGCAAGGTTTACGGCACGGTCGATTTCATCTTTGGAAACTCAGCAGTAGTCCTACAAAACTGCAAAATTGCACCAAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCCGGTTTGATCCTAATCAACACACTGGCATTTCAATACAACACTCTTCAATCCAACCGTTCGAAAACTTGAAAACTACCGAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACGACCGTGTTTATGCAGAACGATTTCGGTAGCTTGATTCACCCAACTGGCTGGCTGCCATGGGTTGGAACATCAGCACCAGACACCATCTTCTATGCTGAGTTTCAAAACAAAGGCCCTGGTTCTTCCACAGCCAACAGAGTGAAATGGAAAGGTTTGAAGAATATTGACACCAAAACTGCCAAGAAATTCACTGTTTCCTCTTTCATTCACGGTAAAGATTGGATCTCAAAGGCTCAAGTCCCCTTCAATGCCACCCTCTGA

Coding sequence (CDS)

ATGTCTCTCCTTAGACCTTATGGTAAGGTCGATGAATCCGATCAAATGAGGCTCGACGCTAGCCGAAGGACTCGGAAGAGGGTCATTGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTTGTGTTCGGGACTAGAAATAGTTCCAAGAACGACGAGAGTGACAATCACAATGAGGCTCTTTCAACTTCGATAAAAGCAGTTTGTGATGAGACTTTGTATCCCGATACTTGTAAGAATGCTTTTGGTTCTCTAGCTAACTCGTCCCACCTCGACCCTGGTCAAATAGCCAAGTTTTCGGTTCAACTCGCGCTCGGTGAACTGTCGCGAGTGGCTGATTACCTTTTGGAGCATGCCATTACCAATTCAACTGATAATAAGACCATATTAGCTTTGAGAAGTTGTCATGAACTTTTGGACTTGGCAATAGATCATCTAAATGACTCATTATCATCAAGTGAGATTACAGTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAAGTTCAGCAGCTACTTACCAGCAAACTTGCATCGACGGGCTCGAGGAAGTCGATCTCGCTTTGACGAATGTCATCGCAAATTACTTGAAGAACTCTACTGAAATGACCAGCAATGGCCTTGCCATTGTGTCCTTCTTTTCGAAGCTAACGGATTCTTTCAGTCTACGACGATTGATGAGTTATGAAGATCGACATAGCAATGGAGATTGGGCGCGCCCGATACTCCGGAAGCTAGCTCAAAAGGATCTCCGGAAGCAGGCCGACATCGTCGTAGCGAAAGACAAATCGGGGAAGTACAAAACAATCACTGAAGCTCTCAATGCTGTACCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTGAAGAAGGGAGTTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTAGTGATGATTGGCGATGGAATGACTTCAACCACTGTAACTGGCAGGCTCAACTTCATAGATGGGACGCCAACATTTTCTACTGCAACATTTGCTGCAAAGGGAAAAGGCTTCATTGCCATAGACATGGGATTTGAGAACACAGCTGGACCGAGCAAACACCAGGCGGTTGCTCTGATGTCGACGTCGGACCAATCGATCTTCTACCGCTGCCAAATGAATGCATACCAAGACACTCTCTATGCACACTCGAACCGTCAATTCTACCGTGACTGCAAGGTTTACGGCACGGTCGATTTCATCTTTGGAAACTCAGCAGTAGTCCTACAAAACTGCAAAATTGCACCAAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCCGGTTTGATCCTAATCAACACACTGGCATTTCAATACAACACTCTTCAATCCAACCGTTCGAAAACTTGAAAACTACCGAAACTTACCTCGGTCGTCCATGGAAGAACTACTCGACGACCGTGTTTATGCAGAACGATTTCGGTAGCTTGATTCACCCAACTGGCTGGCTGCCATGGGTTGGAACATCAGCACCAGACACCATCTTCTATGCTGAGTTTCAAAACAAAGGCCCTGGTTCTTCCACAGCCAACAGAGTGAAATGGAAAGGTTTGAAGAATATTGACACCAAAACTGCCAAGAAATTCACTGTTTCCTCTTTCATTCACGGTAAAGATTGGATCTCAAAGGCTCAAGTCCCCTTCAATGCCACCCTCTGA

Protein sequence

MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDNHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYSTTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTAKKFTVSSFIHGKDWISKAQVPFNATL
Homology
BLAST of CmoCh13G005180 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 6.9e-168
Identity = 308/569 (54.13%), Postives = 407/569 (71.53%), Query Frame = 0

Query: 6   PYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHN 65
           PYGKVDE + +RL+A R+TRK + +I +S V+L G+V+  VFGT   + S +  E++N+ 
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  EALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEH 125
           +++S S+KAVCD TL+ + C    GS  N+S L+P ++ +++V++ + E+S+       +
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI-----N 123

Query: 126 AITNST-DNKTILALRSCHELLDLAIDHLNDSLSSS---EITVLKAVDDLKTWLSSAATY 185
           A ++S  D K  + + +C ELLDL ID+LN++L+SS   ++TV + VDDL+TWLSSA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL-RRLMSYEDRH 245
           Q+TC++ L      +     ++LKNSTE+TSN LAI+++  K+ DSF L RRL++  D  
Sbjct: 184 QRTCVETLAP---DMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 305
            +    R   R L   DLRK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKGV
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFEN 365
           Y ENV+V K  WNV+++GDG + + V+GRLN IDGTPTF TATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAV 425
           TAGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYR+C + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKN 485
           VLQ+C+I P+ P+ GQ+NTITAQGR DPN +TGISI   +I P  +L    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDT 545
           +STTV M +     I   GWLPW G SAPDTIFY E++N GPG+ST NRVKWKGL+ + T
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFIHGKDWISKAQVPFNATL 567
           K A +FTV  FI G  W+   +VPF + L
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of CmoCh13G005180 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 9.1e-168
Identity = 309/568 (54.40%), Postives = 411/568 (72.36%), Query Frame = 0

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT--RNSSKNDESDNHNEA 66
           YG++DE +Q +L+ASR+T+KR+ +I +SS+VLV +VV  V GT  R++SK   ++N+ E 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL--EH 126
           +S S+KA+CD TL+ + C    GS  N+S   P ++ K++V++ + ELS+V D     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQTC 186
                 DN T  A+ +C EL+ LA+D LN++++SS    LK  DDL+TWLSS  TYQ+TC
Sbjct: 124 -----MDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IDGLEEVDL-ALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNGD 246
           +D L E +  +LT    N+LKNSTEMTSN LAI+++  K+ D+   RR    E  ++   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WA-RPIL---RKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 306
            A  P++   R L   DL+K+A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKGV
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFEN 366
           Y ENV V K KWNVVM+GDG + T V+  LNFIDGTPTF TATFA  GKGF+A DMGF N
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFIN 363

Query: 367 TAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAV 426
           TAGP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYRDC + GTVDFIFGN+AV
Sbjct: 364 TAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAV 423

Query: 427 VLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKN 486
           V Q C+I P+ P+ GQ+NTITAQGR DPNQ+TGISI + +I+P +NL   +T+LGRPWK+
Sbjct: 424 VFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKD 483

Query: 487 YSTTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLK-NID 546
           +STTV M++     I+P GWLPW G +APDTIFYAE+ N GPG+ST NRVKW+GLK ++ 
Sbjct: 484 FSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLT 543

Query: 547 TKTAKKFTVSSFIHGKDWISKAQVPFNA 565
            K A KFTV  FI G +W+   +VPFN+
Sbjct: 544 KKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CmoCh13G005180 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.8e-139
Identity = 278/583 (47.68%), Postives = 375/583 (64.32%), Query Frame = 0

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDNHNEAL- 66
           YGKV+E +Q   +  ++TRKR+I+I +SS+VL+ V++A V G    ++N ES   ++++ 
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  ------STSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 126
                 + S+KAVCD T YP +C ++  SL  S+  DP  + K S+++A+ ELS     L
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKL 129

Query: 127 LEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLS-----SSEITVLKAVDDLKTWLSS 186
             +A     D +   A+  C  +   A+D LNDS+S     +  I    +V +++TWLS+
Sbjct: 130 RANA---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIDGLEEVDLALT----NVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL--- 246
           A T Q TC+D + E++          I   ++NSTE  SN LAIV+    L   F     
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249

Query: 247 -RRLMSYEDRHSNGDWARPILRK-LAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKS 306
            RRL+ +       +W     R+ L +K+     D VVAKD SG++KTI EAL  V  KS
Sbjct: 250 HRRLLGFP------EWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKS 309

Query: 307 KKRTVIYVKKGVYKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKG 366
           ++R  +YVK+G Y EN+++ KN WNV++ GDG   T V G  NF+DGTPTF TATFA KG
Sbjct: 310 EERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKG 369

Query: 367 KGFIAIDMGFENTAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVY 426
           KGFIA D+GF N AG SKHQAVAL S SD+S+F+RC  + +QDTLYAHSNRQFYRDC + 
Sbjct: 370 KGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDIT 429

Query: 427 GTVDFIFGNSAVVLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLK 486
           GT+DFIFGN+AVV Q+CKI P+ PL  Q NTITAQG+ DPNQ+TGI IQ S+I PF N  
Sbjct: 430 GTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNL 489

Query: 487 TTETYLGRPWKNYSTTVFMQNDFGSLIHPTGWLPWV-GTSAPDTIFYAEFQNKGPGSSTA 546
           T  TYLGRPWK++STTV MQ+D G+L++P GW+ WV     P TIFYAE+QN GPG+  +
Sbjct: 490 TAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVS 549

Query: 547 NRVKWKGLK-NIDTKTAKKFTVSSFIHGKDWISKAQVPFNATL 567
            RVKW G K  I  + A++FTV SFI G +W+  A V F++TL
Sbjct: 550 QRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of CmoCh13G005180 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 5.6e-125
Identity = 259/575 (45.04%), Postives = 359/575 (62.43%), Query Frame = 0

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHNE 66
           YGKVDE+  + L   ++TRKR++++ +S VVL+ V++A V  T   +N +++  S     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEHA 126
             STS+KA+C  T +P++C ++   L +S+  DP  + K S+++ + EL  ++D L E  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLK------AVDDLKTWLSSAAT 186
              + D +   ALR C +L++ A+D LND++S+ +    K       ++DLKTWLS+  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDGLEEV--------DLALTNVIANYLKNSTEMTSNGLAIVS-FFSKLTD----S 246
             +TC D L+E+        +  +T  + + +  STE TSN LAIVS   S L+D     
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 FSLRRLMSYEDRHSNG--DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVP 306
              RRLMS+  + S     WAR   R+L Q    K  D+ VA D +G   T+ EA+  VP
Sbjct: 250 HRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVP 309

Query: 307 DKSKKRTVIYVKKGVYKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFA 366
            KS K  VIYVK G Y ENV + K+KWNV++ GDG   T ++G  NF+DGTPT+ TATFA
Sbjct: 310 KKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFA 369

Query: 367 AKGKGFIAIDMGFENTAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDC 426
            +GKGFI  D+G  NTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYRDC
Sbjct: 370 IQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDC 429

Query: 427 KVYGTVDFIFGNSAVVLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFE 486
            V GT+DFIFG++AVV Q CKI P+ PL  Q NTITAQG+ DPNQ +G+SIQ  +I    
Sbjct: 430 DVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANG 489

Query: 487 NLKTTETYLGRPWKNYSTTVFMQNDFGSLIHPTGWLPWV-GTSAPDTIFYAEFQNKGPGS 546
           N+    TYLGRPWK +STTV M+   G+++ P+GW+ WV G   P +I Y E++N GPGS
Sbjct: 490 NV-IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGS 549

Query: 547 STANRVKWKGLKNI-DTKTAKKFTVSSFIHGKDWI 556
               RVKW G K +     A KFTV++ +HG DWI
Sbjct: 550 DVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of CmoCh13G005180 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 8.9e-107
Identity = 228/571 (39.93%), Postives = 331/571 (57.97%), Query Frame = 0

Query: 16  MRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDE-SDNHNEALSTSIKAVCD 75
           M +D   + +K +I  V+++++++ VV   +  +RN+S +++      +  +T+++AVC 
Sbjct: 1   MGVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCA 60

Query: 76  ETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGEL-SRVADYLLEHAITNSTDNKTI 135
            T Y +TC N+    A+     P  + K    + +  +   +    +E     + D  T 
Sbjct: 61  PTDYKETCVNSL-MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTK 120

Query: 136 LALRSCHELLDLAIDHLNDSLSS----SEITVLKAVDDLKTWLSSAATYQQTCIDGLEEV 195
            AL  C +L++ A D L   L +    S   +   V+DL+ WLS +  YQQTC+D  EE 
Sbjct: 121 GALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 180

Query: 196 DLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL-----------RRLMSYEDRHS 255
           +  L+  +    K S E+TSNGLA+++  S L   F++           R+L+S ED   
Sbjct: 181 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED--G 240

Query: 256 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVY 315
              W  P  R+L       +A++VVA D SG+YKTI EALNAVP  ++K  VIY+K+GVY
Sbjct: 241 IPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVY 300

Query: 316 KENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDG-TPTFSTATFAAKGKGFIAIDMGFEN 375
            E V+V K   +V  IGDG T T +TG LN+  G   T+ TAT A  G  F A ++GFEN
Sbjct: 301 NEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFEN 360

Query: 376 TAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAV 435
           TAGP  HQAVAL  ++D ++FY CQ++ YQDTLY HS+RQF+RDC V GTVDFIFG+  V
Sbjct: 361 TAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIV 420

Query: 436 VLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQ------PFENLKTTETYL 495
           VLQNC I  + P+  Q   ITAQGR D  + TG+ +Q+  I       P +++   + YL
Sbjct: 421 VLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSI--NKAYL 480

Query: 496 GRPWKNYSTTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKG 555
           GRPWK +S T+ M      +I P GWLPW G  A +T++YAE++N GPGS+ A RVKW G
Sbjct: 481 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 540

Query: 556 LKNIDTKTAKKFTVSSFIHGKDWISKAQVPF 563
           +K +  K A +FT + F+ G  WI   +VP+
Sbjct: 541 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CmoCh13G005180 vs. ExPASy TrEMBL
Match: A0A6J1ENB0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
           HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
           EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
           TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
           DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
           NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
           NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
           TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFNATL 567
           KKFTVSSFIHGKDWISKAQVPFNATL
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of CmoCh13G005180 vs. ExPASy TrEMBL
Match: A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 553/566 (97.70%), Postives = 558/566 (98.59%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
           HNEALSTSIKAVCDETLYPDTC NAFG LANSSHLDPGQIAKFSVQLAL ELSRVADYLL
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
           EHAITNSTDNKTILALR CHELLDLA+DHLNDSLSSSEIT+LKAVDDLKTWLSSAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
           TCIDGLEEVDLAL NVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
           DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEAL AV DKSKKRTVIYVKKGVYKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
           NVEVVKNKWNVVMIGDGMTSTTVTG+LNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
           NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTT+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480

Query: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
           TVFMQN+FGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFNATL 567
           KKFTVSSFI GKDWISKAQVPFNATL
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of CmoCh13G005180 vs. ExPASy TrEMBL
Match: A0A0A0LY08 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.7e-278
Identity = 482/567 (85.01%), Postives = 528/567 (93.12%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRN-SSKNDESD 60
           MS LRPYGKVDE+DQ+RLDA RRTR+R++VIVLSSVVLV VVVAVVFGTRN S+ ND+S+
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120
           N N +LSTSIKAVCD TLYPDTC+ AFG +ANSSHLDPGQI+K SVQLALGELS+VADY+
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 LEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180
            +HAIT + DNKTILAL++CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTW++SAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240
           QTCID L EVD AL +++AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYE+  SN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300
           GDWARPILRKLAQ D+RK ADIVVAKD+SGKYKTITEAL AVPDKSKKRTVIYVKKG+YK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360
           ENVEVVKNKWNVVM+GDGMT+T V+G LN +DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+MNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF +LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540
           TTVFMQ++ G LIHPTGWLPW+GTSAPDTIFYAEFQN GPGSST+ RVKWKG+KNIDTKT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFNATL 567
           AKKFTVSSFI GKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of CmoCh13G005180 vs. ExPASy TrEMBL
Match: A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.9e-277
Identity = 484/567 (85.36%), Postives = 527/567 (92.95%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRN-SSKNDESD 60
           MS LRPYGKVDE+DQ+RLDASRRTR+R++VIVLSSVVLV VVVAVVFGTRN S+ ND+S+
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120
           N N +LS SIKAVCD TLYPDTC+ AFG  ANSSHLDPGQI+K +VQLALGELS+VAD++
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 LEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180
            +HAITN+TD+KTILAL++CHELLDLA+DHLN +LSSS+IT+LKAVDDLKTWL+SAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240
           QTCIDGL EVD AL ++ AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYEDR SN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300
           GDWARPILRKLAQ D+RKQADIVVAKD SGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360
           E VEVVKNKWNVVM+GDGM +T V+G LN IDGTPTFSTA+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF+NLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540
           TTVFMQ++ GSLIHP+GWLPWVGTSAPDTIFYAEFQN G GSST+ RVKWKG+KNID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFNATL 567
           AKKFTVSSFI GKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of CmoCh13G005180 vs. ExPASy TrEMBL
Match: A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.9e-277
Identity = 484/567 (85.36%), Postives = 527/567 (92.95%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRN-SSKNDESD 60
           MS LRPYGKVDE+DQ+RLDASRRTR+R++VIVLSSVVLV VVVAVVFGTRN S+ ND+S+
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120
           N N +LS SIKAVCD TLYPDTC+ AFG  ANSSHLDPGQI+K +VQLALGELS+VAD++
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 LEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180
            +HAITN+TD+KTILAL++CHELLDLA+DHLN +LSSS+IT+LKAVDDLKTWL+SAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240
           QTCIDGL EVD AL ++ AN+LKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYEDR SN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300
           GDWARPILRKLAQ D+RKQADIVVAKD SGKYKTITEAL AVPDKSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360
           E VEVVKNKWNVVM+GDGM +T V+G LN IDGTPTFSTA+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ S IQPF+NLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540
           TTVFMQ++ GSLIHP+GWLPWVGTSAPDTIFYAEFQN G GSST+ RVKWKG+KNID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFNATL 567
           AKKFTVSSFI GKDWISKAQVPF ATL
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of CmoCh13G005180 vs. NCBI nr
Match: XP_022927225.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata])

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
           HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
           EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
           TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
           DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
           NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
           NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
           TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFNATL 567
           KKFTVSSFIHGKDWISKAQVPFNATL
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of CmoCh13G005180 vs. NCBI nr
Match: KAG6583848.1 (putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1113.2 bits (2878), Expect = 0.0e+00
Identity = 564/566 (99.65%), Postives = 565/566 (99.82%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
           HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
           EHAITNSTDNKTILALRSCHELLDLA+DHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAMDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
           TCIDGLEEVDLAL NVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
           DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
           NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
           NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
           TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFNATL 567
           KKFTVSSFIHGKDWISKAQVPFNATL
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of CmoCh13G005180 vs. NCBI nr
Match: XP_023520176.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 562/566 (99.29%), Postives = 564/566 (99.65%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
           HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
           EHAITNSTDNKTILALRSCHELL+LAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
           TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSY+DRHSNG
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSNG 240

Query: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
           DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
           NVEVVKNKWNVVMIGDGMT TTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTLTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
           NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
           TVFMQNDFG LIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA
Sbjct: 481 TVFMQNDFGRLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFNATL 567
           KKFTVSSFIHGKDWISKAQVPFNATL
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of CmoCh13G005180 vs. NCBI nr
Match: XP_023000829.1 (probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 553/566 (97.70%), Postives = 558/566 (98.59%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60
           MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120
           HNEALSTSIKAVCDETLYPDTC NAFG LANSSHLDPGQIAKFSVQLAL ELSRVADYLL
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120

Query: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180
           EHAITNSTDNKTILALR CHELLDLA+DHLNDSLSSSEIT+LKAVDDLKTWLSSAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240
           TCIDGLEEVDLAL NVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300
           DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEAL AV DKSKKRTVIYVKKGVYKE
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360
           NVEVVKNKWNVVMIGDGMTSTTVTG+LNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480
           NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTT+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480

Query: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540
           TVFMQN+FGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFNATL 567
           KKFTVSSFI GKDWISKAQVPFNATL
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of CmoCh13G005180 vs. NCBI nr
Match: XP_038895610.1 (putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida])

HSP 1 Score: 979.2 bits (2530), Expect = 1.5e-281
Identity = 494/567 (87.13%), Postives = 531/567 (93.65%), Query Frame = 0

Query: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRN-SSKNDESD 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLVGVVVAVVFGTRN S+ ND+S+
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60

Query: 61  NHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYL 120
           N + ALSTSIKAVCD TLYP TC+NAFG LANSSHLDP QI+K SVQLALGELSRVADY+
Sbjct: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120

Query: 121 LEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQ 180
             HAITNSTDNKTILAL++CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTWL+SAATYQ
Sbjct: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240
           QTCIDGL EVD AL N+IANYLKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYED  SN
Sbjct: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240

Query: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYK 300
           GDWARPILRKLAQ+D+RKQADIVVAKD SGKYKTIT AL AVP+KSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360
           ENVEVVKNKWNVV++GDGMT+T VTG LN IDGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRCQM+AYQDTLY+HSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ SSIQP+ENLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540
           TTVFMQ++ GSLIHP+GWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKW G+KNID KT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFNATL 567
           AKKFTVSSFI GKDWISKAQVPF A++
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 567

BLAST of CmoCh13G005180 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 592.0 bits (1525), Expect = 4.9e-169
Identity = 308/569 (54.13%), Postives = 407/569 (71.53%), Query Frame = 0

Query: 6   PYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHN 65
           PYGKVDE + +RL+A R+TRK + +I +S V+L G+V+  VFGT   + S +  E++N+ 
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  EALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEH 125
           +++S S+KAVCD TL+ + C    GS  N+S L+P ++ +++V++ + E+S+       +
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI-----N 123

Query: 126 AITNST-DNKTILALRSCHELLDLAIDHLNDSLSSS---EITVLKAVDDLKTWLSSAATY 185
           A ++S  D K  + + +C ELLDL ID+LN++L+SS   ++TV + VDDL+TWLSSA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL-RRLMSYEDRH 245
           Q+TC++ L      +     ++LKNSTE+TSN LAI+++  K+ DSF L RRL++  D  
Sbjct: 184 QRTCVETLAP---DMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 305
            +    R   R L   DLRK ADIVVAKD SGKY+TI  AL  VP+KS+KRT+IYVKKGV
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFEN 365
           Y ENV+V K  WNV+++GDG + + V+GRLN IDGTPTF TATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAV 425
           TAGPSKHQAVALM ++D + FYRC MNAYQDTLY H+ RQFYR+C + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKN 485
           VLQ+C+I P+ P+ GQ+NTITAQGR DPN +TGISI   +I P  +L    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDT 545
           +STTV M +     I   GWLPW G SAPDTIFY E++N GPG+ST NRVKWKGL+ + T
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFIHGKDWISKAQVPFNATL 567
           K A +FTV  FI G  W+   +VPF + L
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of CmoCh13G005180 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 591.7 bits (1524), Expect = 6.4e-169
Identity = 309/568 (54.40%), Postives = 411/568 (72.36%), Query Frame = 0

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT--RNSSKNDESDNHNEA 66
           YG++DE +Q +L+ASR+T+KR+ +I +SS+VLV +VV  V GT  R++SK   ++N+ E 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL--EH 126
           +S S+KA+CD TL+ + C    GS  N+S   P ++ K++V++ + ELS+V D     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQTC 186
                 DN T  A+ +C EL+ LA+D LN++++SS    LK  DDL+TWLSS  TYQ+TC
Sbjct: 124 -----MDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IDGLEEVDL-ALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNGD 246
           +D L E +  +LT    N+LKNSTEMTSN LAI+++  K+ D+   RR    E  ++   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WA-RPIL---RKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGV 306
            A  P++   R L   DL+K+A IVVAKD SGKY+TI EAL  V +K++K T+IYVKKGV
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFEN 366
           Y ENV V K KWNVVM+GDG + T V+  LNFIDGTPTF TATFA  GKGF+A DMGF N
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFIN 363

Query: 367 TAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAV 426
           TAGP+KHQAVALM ++D S+FY+C M+A+QDT+YAH+ RQFYRDC + GTVDFIFGN+AV
Sbjct: 364 TAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAV 423

Query: 427 VLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKN 486
           V Q C+I P+ P+ GQ+NTITAQGR DPNQ+TGISI + +I+P +NL   +T+LGRPWK+
Sbjct: 424 VFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKD 483

Query: 487 YSTTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLK-NID 546
           +STTV M++     I+P GWLPW G +APDTIFYAE+ N GPG+ST NRVKW+GLK ++ 
Sbjct: 484 FSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLT 543

Query: 547 TKTAKKFTVSSFIHGKDWISKAQVPFNA 565
            K A KFTV  FI G +W+   +VPFN+
Sbjct: 544 KKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CmoCh13G005180 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 449.5 bits (1155), Expect = 3.9e-126
Identity = 259/575 (45.04%), Postives = 359/575 (62.43%), Query Frame = 0

Query: 7   YGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGT---RNSSKNDESDNHNE 66
           YGKVDE+  + L   ++TRKR++++ +S VVL+ V++A V  T   +N +++  S     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLLEHA 126
             STS+KA+C  T +P++C ++   L +S+  DP  + K S+++ + EL  ++D L E  
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD-LPEKL 129

Query: 127 ITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLK------AVDDLKTWLSSAAT 186
              + D +   ALR C +L++ A+D LND++S+ +    K       ++DLKTWLS+  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDGLEEV--------DLALTNVIANYLKNSTEMTSNGLAIVS-FFSKLTD----S 246
             +TC D L+E+        +  +T  + + +  STE TSN LAIVS   S L+D     
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 FSLRRLMSYEDRHSNG--DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVP 306
              RRLMS+  + S     WAR   R+L Q    K  D+ VA D +G   T+ EA+  VP
Sbjct: 250 HRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVP 309

Query: 307 DKSKKRTVIYVKKGVYKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFA 366
            KS K  VIYVK G Y ENV + K+KWNV++ GDG   T ++G  NF+DGTPT+ TATFA
Sbjct: 310 KKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFA 369

Query: 367 AKGKGFIAIDMGFENTAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDC 426
            +GKGFI  D+G  NTAG +KHQAVA  S SD S++Y+C  + +QDTLY HSNRQFYRDC
Sbjct: 370 IQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDC 429

Query: 427 KVYGTVDFIFGNSAVVLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFE 486
            V GT+DFIFG++AVV Q CKI P+ PL  Q NTITAQG+ DPNQ +G+SIQ  +I    
Sbjct: 430 DVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANG 489

Query: 487 NLKTTETYLGRPWKNYSTTVFMQNDFGSLIHPTGWLPWV-GTSAPDTIFYAEFQNKGPGS 546
           N+    TYLGRPWK +STTV M+   G+++ P+GW+ WV G   P +I Y E++N GPGS
Sbjct: 490 NV-IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGS 549

Query: 547 STANRVKWKGLKNI-DTKTAKKFTVSSFIHGKDWI 556
               RVKW G K +     A KFTV++ +HG DWI
Sbjct: 550 DVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of CmoCh13G005180 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 389.0 bits (998), Expect = 6.3e-108
Identity = 228/571 (39.93%), Postives = 331/571 (57.97%), Query Frame = 0

Query: 16  MRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDE-SDNHNEALSTSIKAVCD 75
           M +D   + +K +I  V+++++++ VV   +  +RN+S +++      +  +T+++AVC 
Sbjct: 1   MGVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCA 60

Query: 76  ETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGEL-SRVADYLLEHAITNSTDNKTI 135
            T Y +TC N+    A+     P  + K    + +  +   +    +E     + D  T 
Sbjct: 61  PTDYKETCVNSL-MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTK 120

Query: 136 LALRSCHELLDLAIDHLNDSLSS----SEITVLKAVDDLKTWLSSAATYQQTCIDGLEEV 195
            AL  C +L++ A D L   L +    S   +   V+DL+ WLS +  YQQTC+D  EE 
Sbjct: 121 GALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 180

Query: 196 DLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL-----------RRLMSYEDRHS 255
           +  L+  +    K S E+TSNGLA+++  S L   F++           R+L+S ED   
Sbjct: 181 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED--G 240

Query: 256 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVY 315
              W  P  R+L       +A++VVA D SG+YKTI EALNAVP  ++K  VIY+K+GVY
Sbjct: 241 IPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVY 300

Query: 316 KENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDG-TPTFSTATFAAKGKGFIAIDMGFEN 375
            E V+V K   +V  IGDG T T +TG LN+  G   T+ TAT A  G  F A ++GFEN
Sbjct: 301 NEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFEN 360

Query: 376 TAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAV 435
           TAGP  HQAVAL  ++D ++FY CQ++ YQDTLY HS+RQF+RDC V GTVDFIFG+  V
Sbjct: 361 TAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIV 420

Query: 436 VLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQ------PFENLKTTETYL 495
           VLQNC I  + P+  Q   ITAQGR D  + TG+ +Q+  I       P +++   + YL
Sbjct: 421 VLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSI--NKAYL 480

Query: 496 GRPWKNYSTTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKG 555
           GRPWK +S T+ M      +I P GWLPW G  A +T++YAE++N GPGS+ A RVKW G
Sbjct: 481 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 540

Query: 556 LKNIDTKTAKKFTVSSFIHGKDWISKAQVPF 563
           +K +  K A +FT + F+ G  WI   +VP+
Sbjct: 541 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CmoCh13G005180 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 386.7 bits (992), Expect = 3.1e-107
Identity = 229/513 (44.64%), Postives = 307/513 (59.84%), Query Frame = 0

Query: 66  STSIKAVCDETLYPDTCKNAFGSL-ANSSHLDPGQIAKFSVQLALGELSRVADYLLEHAI 125
           S+ ++ VC+ T YP +C ++   L  + +  DP  + + S+Q+   EL+ +   L +   
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 126 TNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITV--------LKAVDDLKTWLSSAA 185
             + D     AL  C ++ DLA+D +ND++SS +  +           + DL TWLSSA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 186 TYQQTCIDGLEE--VDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSL----RRL 245
           T   TC D L+E   +  +   + + + NSTE TSN LAIV+   K      +    RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 246 MSYEDRHSNGDWARPILRKLAQ-KDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRT 305
           +   + +S  +W RP +R+L Q K+L     + VA D SG  +T+ EA+  VP K K   
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAKNLTPH--VTVAADGSGDVRTVNEAVWRVPKKGKTMF 695

Query: 306 VIYVKKGVYKENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFI 365
           VIYVK G Y ENV + K+KWNV + GDG   T ++G  N +DG  TF+T+TFA +GKGF+
Sbjct: 696 VIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFM 755

Query: 366 AIDMGFENTAGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVD 425
             DMG  NTAGP KHQAVA  S SD+S++YRC  + YQDTLY HSNRQ+YR+C V GTVD
Sbjct: 756 MKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVD 815

Query: 426 FIFGNSAVVLQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTET 485
           FIFG   VV Q C I P+ PL  Q NTITA+G  + NQ+TGISI   +I P  N+ T  T
Sbjct: 816 FIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNV-TATT 875

Query: 486 YLGRPWKNYSTTVFMQNDFGSLIHPTGWLPWVGT--SAPDTIFYAEFQNKGPGSSTANRV 545
           YLGRPWK +S TV MQ+  GS ++P GW+ W  T    P TIFY E++N GPGS  + RV
Sbjct: 876 YLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRV 935

Query: 546 KWKGLKNIDT-KTAKKFTVSSFIHGKD-WISKA 559
           KW G K I +   A +FTV  F+ G D WI KA
Sbjct: 936 KWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SG776.9e-16854.13Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3... [more]
Q9FF789.1e-16854.40Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3... [more]
Q431111.8e-13947.68Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1[more]
Q438675.6e-12545.04Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1[more]
Q9FJ218.9e-10739.93Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1ENB00.0e+00100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1[more]
A0A6J1KL230.0e+0097.70Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1[more]
A0A0A0LY081.7e-27885.01Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1[more]
A0A5A7VDG11.9e-27785.36Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G0077... [more]
A0A1S3BDA41.9e-27785.36Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022927225.10.0e+00100.00putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata][more]
KAG6583848.10.0e+0099.65putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosp... [more]
XP_023520176.10.0e+0099.29putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita pepo subsp. pepo][more]
XP_023000829.10.0e+0097.70probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima][more]
XP_038895610.11.5e-28187.13putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G10710.14.9e-16954.13root hair specific 12 [more]
AT5G04960.16.4e-16954.40Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53840.13.9e-12645.04pectin methylesterase 1 [more]
AT5G49180.16.3e-10839.93Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14300.13.1e-10744.64pectinesterase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 64..214
e-value: 2.5E-34
score: 130.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 68..214
e-value: 2.4E-27
score: 96.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 38..215
e-value: 3.6E-24
score: 83.0
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 260..551
e-value: 1.8E-139
score: 463.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 66..218
e-value: 7.2E-33
score: 115.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 68..219
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 255..566
e-value: 1.8E-112
score: 377.9
NoneNo IPR availablePANTHERPTHR31707:SF272PECTINESTERASE/PECTINESTERASE INHIBITOR 24-RELATEDcoord: 19..562
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 19..562
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 71..220
e-value: 1.25008E-39
score: 139.881
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 285..304
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 404..413
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 258..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G005180.1CmoCh13G005180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity