CmoCh13G004710 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G004710
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionE3 ubiquitin-protein ligase arih1
LocationCmo_Chr13: 5764384 .. 5767359 (+)
RNA-Seq ExpressionCmoCh13G004710
SyntenyCmoCh13G004710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAGAAACCAACTTATTAGCTTTAATCAATCTTACGCCATCGAATTGGCAAGTAGTAGCTCAAGATTCTTTCTTTTGATTCATTTTGCTCTCTGCAAACCCCAATCAATGGATCCACCTGTTTTCCACACTGATTTTGTGAATCGGGAGCGAGAAATTTTGGGCACTGCTTTGGCGCTCTTTCTTGCTGTTCGTCACATTAGGGTTTTTTAATTTCTTGTTTCTACACACAAATTTTTCACTCCCCAGAATTCTCTTCACATGAGGTATGGAATCTTCCATTCTTTGAATACTTTATCTGTTTCTCTTTCTTTGTTCTTCATTCTCCAACCCCTAATCCTGAAGTTTGTGTAGTTTTTTTTGTTGATGCGATTAAATGGGGTTTGATAAATTAGTTGGTTGCCCAGTGGGTCGATCTTGATTTGCGCAGAAAAATACAAGAATTACTTGTTTTATTTGAATTTTCTCCAATACGGACATGTTCTTCTTTTCATTATTCGATTTTGGGAATTTCATGTCCTCGATGTTGAAATTTCACGAATCTTAATCCTTTTAGGTTGATTCTACAATGGTTATTTTGTTACTTTTTGATGTAGATCGTTTTCTGTGATTTTAGGAGTGATTGCTGCTTCAATTTAAAAATTAGGGAAAGATTGCCTGGGAGTTCTTTTTTTGGACTCCTCGATTAGCGGTGGCTATGAAATCCTTCACGTGATTCTTTTGAGTAATTAAGAAGGGGGAAGAACAGTCTTAAATTCATTTGGTGGTGGGTATTCTCTTATTTACTGCCACTTTAATCCTAAACCATCTGGGGTTTGTGGATTGTTTGAAAAATTTGGAGAATGGATCATCGTGAGATTTTTGGCCGTGGTTCTTCTCGTGTTCTAGCTGAGGACGATGATGGTGAAGATTTCCGAAGTTGTTGTGAAGATCACGAAGTCTGGAAAGATAATGAATTTGCAGGGGAAGAACAGTCTGAGAAGGAAGAGGATCTTGATGAGTTTTCCGTGAAATTGTTCTTTAAAGGCGTTTCGATAGCGGGGATCGGCGATCCTGCTTCTGGTCTTTCTGGAATTGGTGTATTCATGGAGAAATCAGGAAAGGTCCCTTTTATTCAGGTCCAGAAGAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTAGTTGAGGCTATGCAGAACGACATCCACAGTATTCGAGCTTTCACCGACTCCGATCCGTTGTATAAACAGGTTATTACTTCCCGACCCACCTAATCTTTTCTTGTTATGATTTATGCTATAATATCTGTGGAAGTTCAAGAGAGTGGAGAGATGCATAAACTCTTAGAGTTTTCTGGCCATGTTCATACATCTATTATGCTTAAGATAATGTGAATGTTTGACAGATAACAAATGAAGAACAGCCTGAAATTCCACTTCTTATAGCGCTGAAAGAAAGGATTCTAGAACATGCCAGCAATTTTGAAGCCTTTTCCCTAAACCTTTCTTCAAGTACTGATCTCGAGCAGCCATTTCATCTTGCGAAAGTGGCTATCGGGGTTCTTTCTCGTCCGAAAAAAGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGATAATAAACCATCTGCCATGATTGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTAATTGTTTGAAGACTTATGTTGATGGAAAAGTAGAGGCATCCCAAGTTCCCATTAGATGCCCTCAATTGAGATGCCAATACTACATTACAAACAATGAGTTTAGATCTTTTCTTCCCCTGACTTTATATGAATCATTAGATAATACCTTAGCAGAAGCAAACCTTCGCTCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCGAGCGAATGTTTGTCAGCTAGGGCAAGTTCATCAAGTCAATCGGATAATAGCTGTTTCGAGTGTCCTGTATGTCAGAGATTCATCTGTGTTGAATGTCAAGTTCCTTGGCACTCGTCGATGAGCTGCGAAGAATTTCAGAACGTCCCGTTAGAGGAGAGAGACACTGCTGATATTACCTTACGCCATCTTGCACAAAACAAAAGGTGGCGCCGTTGTCAGGAGTGTCGTAGGATGATTGAATTGACTCAGGGTTGCTACCATATGACATGCTGGTAAGGCTTCTTCCTTCAAATCTTAATTTAGTGCATATTTTATAGCAGCCTTGTTGTCAATGTGATGAACATGATTCGTGTATATAACAAGTAGATCTAACTTATTAGATACAATATTTGCACGTAATACTCGGTTTTTAGAGCTAGTTCGAGCAGGGCTGTATGTATATGACTTCAATGTTTATGCTGCTTTGCTTGATGTGTTTTACAGGTGTGGGCATGAATTCTGCTATTCATGTGGTGGTGAGTATCAAGATGGGCAACAGACGTGCCGATGCACGTTGTGGGACGAAGAAAACTCCGAGGACATGGTCACCCATTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGACACATACTCGGAGCAAGAGAGGTCACAATTAGCACTGATTCAACGATTCCTCGCCGGGGGATTTAGTTTGAGCGACCACCAACCGTATCAATCTCCACCACGATGTACAGACTCTTCTTATGTCGACACGATGAAGGACATACACCAGCTTCCTTGGCTCGAGAGGTTTGTTTCTCTGATAAGCGATAGCTACTATGAAGATTATGTACAATGATATCAATTCTTAGGTGTCTTACTGATATTGAAAAATAAAAGGGGGAAGGTAAAAGAGTTTCTTACTCTAGTTTTCGTCCTTCATTTACACCGCCGAGCTTGGCGTTTCGATGTACTGAATCATGCTGCTGCTGCTGCTTATGATGTGGCCCTCAGAGGCCCTCCTTGCATTAGATTTGAATGGGGGAATCTTATGCAAACAAATGCTTGA

mRNA sequence

AAACAGAAACCAACTTATTAGCTTTAATCAATCTTACGCCATCGAATTGGCAAGTAGTAGCTCAAGATTCTTTCTTTTGATTCATTTTGCTCTCTGCAAACCCCAATCAATGGATCCACCTGTTTTCCACACTGATTTTGTGAATCGGGAGCGAGAAATTTTGGGCACTGCTTTGGCGCTCTTTCTTGCTGTTCGTCACATTAGGGTTTTTTAATTTCTTGTTTCTACACACAAATTTTTCACTCCCCAGAATTCTCTTCACATGAGGAGTGATTGCTGCTTCAATTTAAAAATTAGGGAAAGATTGCCTGGGAGTTCTTTTTTTGGACTCCTCGATTAGCGGTGGCTATGAAATCCTTCACGTGATTCTTTTGAGTAATTAAGAAGGGGGAAGAACAGTCTTAAATTCATTTGGTGGTGGGTATTCTCTTATTTACTGCCACTTTAATCCTAAACCATCTGGGGTTTGTGGATTGTTTGAAAAATTTGGAGAATGGATCATCGTGAGATTTTTGGCCGTGGTTCTTCTCGTGTTCTAGCTGAGGACGATGATGGTGAAGATTTCCGAAGTTGTTGTGAAGATCACGAAGTCTGGAAAGATAATGAATTTGCAGGGGAAGAACAGTCTGAGAAGGAAGAGGATCTTGATGAGTTTTCCGTGAAATTGTTCTTTAAAGGCGTTTCGATAGCGGGGATCGGCGATCCTGCTTCTGGTCTTTCTGGAATTGGTGTATTCATGGAGAAATCAGGAAAGGTCCCTTTTATTCAGGTCCAGAAGAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTAGTTGAGGCTATGCAGAACGACATCCACAGTATTCGAGCTTTCACCGACTCCGATCCGTTGTATAAACAGATAACAAATGAAGAACAGCCTGAAATTCCACTTCTTATAGCGCTGAAAGAAAGGATTCTAGAACATGCCAGCAATTTTGAAGCCTTTTCCCTAAACCTTTCTTCAAGTACTGATCTCGAGCAGCCATTTCATCTTGCGAAAGTGGCTATCGGGGTTCTTTCTCGTCCGAAAAAAGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGATAATAAACCATCTGCCATGATTGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTAATTGTTTGAAGACTTATGTTGATGGAAAAGTAGAGGCATCCCAAGTTCCCATTAGATGCCCTCAATTGAGATGCCAATACTACATTACAAACAATGAGTTTAGATCTTTTCTTCCCCTGACTTTATATGAATCATTAGATAATACCTTAGCAGAAGCAAACCTTCGCTCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCGAGCGAATGTTTGTCAGCTAGGGCAAGTTCATCAAGTCAATCGGATAATAGCTGTTTCGAGTGTCCTGTATGTCAGAGATTCATCTGTGTTGAATGTCAAGTTCCTTGGCACTCGTCGATGAGCTGCGAAGAATTTCAGAACGTCCCGTTAGAGGAGAGAGACACTGCTGATATTACCTTACGCCATCTTGCACAAAACAAAAGGTGGCGCCGTTGTCAGGAGTGTCGTAGGATGATTGAATTGACTCAGGGTTGCTACCATATGACATGCTGGTGTGGGCATGAATTCTGCTATTCATGTGGTGGTGAGTATCAAGATGGGCAACAGACGTGCCGATGCACGTTGTGGGACGAAGAAAACTCCGAGGACATGGTCACCCATTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGACACATACTCGGAGCAAGAGAGGTCACAATTAGCACTGATTCAACGATTCCTCGCCGGGGGATTTAGTTTGAGCGACCACCAACCGTATCAATCTCCACCACGATGTACAGACTCTTCTTATGTCGACACGATGAAGGACATACACCAGCTTCCTTGGCTCGAGAGGGGGAAGGTAAAAGAGTTTCTTACTCTAGTTTTCGTCCTTCATTTACACCGCCGAGCTTGGCGTTTCGATGTACTGAATCATGCTGCTGCTGCTGCTTATGATGTGGCCCTCAGAGGCCCTCCTTGCATTAGATTTGAATGGGGGAATCTTATGCAAACAAATGCTTGA

Coding sequence (CDS)

ATGGATCATCGTGAGATTTTTGGCCGTGGTTCTTCTCGTGTTCTAGCTGAGGACGATGATGGTGAAGATTTCCGAAGTTGTTGTGAAGATCACGAAGTCTGGAAAGATAATGAATTTGCAGGGGAAGAACAGTCTGAGAAGGAAGAGGATCTTGATGAGTTTTCCGTGAAATTGTTCTTTAAAGGCGTTTCGATAGCGGGGATCGGCGATCCTGCTTCTGGTCTTTCTGGAATTGGTGTATTCATGGAGAAATCAGGAAAGGTCCCTTTTATTCAGGTCCAGAAGAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTAGTTGAGGCTATGCAGAACGACATCCACAGTATTCGAGCTTTCACCGACTCCGATCCGTTGTATAAACAGATAACAAATGAAGAACAGCCTGAAATTCCACTTCTTATAGCGCTGAAAGAAAGGATTCTAGAACATGCCAGCAATTTTGAAGCCTTTTCCCTAAACCTTTCTTCAAGTACTGATCTCGAGCAGCCATTTCATCTTGCGAAAGTGGCTATCGGGGTTCTTTCTCGTCCGAAAAAAGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGATAATAAACCATCTGCCATGATTGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTAATTGTTTGAAGACTTATGTTGATGGAAAAGTAGAGGCATCCCAAGTTCCCATTAGATGCCCTCAATTGAGATGCCAATACTACATTACAAACAATGAGTTTAGATCTTTTCTTCCCCTGACTTTATATGAATCATTAGATAATACCTTAGCAGAAGCAAACCTTCGCTCGGATAGAATTTACTGCCCTTTTCCAAATTGCTCTGTCCTGTTAGACCCGAGCGAATGTTTGTCAGCTAGGGCAAGTTCATCAAGTCAATCGGATAATAGCTGTTTCGAGTGTCCTGTATGTCAGAGATTCATCTGTGTTGAATGTCAAGTTCCTTGGCACTCGTCGATGAGCTGCGAAGAATTTCAGAACGTCCCGTTAGAGGAGAGAGACACTGCTGATATTACCTTACGCCATCTTGCACAAAACAAAAGGTGGCGCCGTTGTCAGGAGTGTCGTAGGATGATTGAATTGACTCAGGGTTGCTACCATATGACATGCTGGTGTGGGCATGAATTCTGCTATTCATGTGGTGGTGAGTATCAAGATGGGCAACAGACGTGCCGATGCACGTTGTGGGACGAAGAAAACTCCGAGGACATGGTCACCCATTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGACACATACTCGGAGCAAGAGAGGTCACAATTAGCACTGATTCAACGATTCCTCGCCGGGGGATTTAGTTTGAGCGACCACCAACCGTATCAATCTCCACCACGATGTACAGACTCTTCTTATGTCGACACGATGAAGGACATACACCAGCTTCCTTGGCTCGAGAGGGGGAAGGTAAAAGAGTTTCTTACTCTAGTTTTCGTCCTTCATTTACACCGCCGAGCTTGGCGTTTCGATGTACTGAATCATGCTGCTGCTGCTGCTTATGATGTGGCCCTCAGAGGCCCTCCTTGCATTAGATTTGAATGGGGGAATCTTATGCAAACAAATGCTTGA

Protein sequence

MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFFKGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITLRHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEENSEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTDSSYVDTMKDIHQLPWLERGKVKEFLTLVFVLHLHRRAWRFDVLNHAAAAAYDVALRGPPCIRFEWGNLMQTNA
Homology
BLAST of CmoCh13G004710 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 9.6e-21
Identity = 66/209 (31.58%), Postives = 94/209 (44.98%), Query Frame = 0

Query: 204 CSICCDNKPSAMIVALKCCHK-FCSNCLKTYVDGKVEASQVPIRCPQLRCQYYITNNEFR 263
           C +C ++KP   I  L CC K  C  CLK Y+  +V+  QV I+CP   C  ++   E  
Sbjct: 236 CRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFL--EETT 295

Query: 264 SFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECP 323
               LT  +S+         R D    P P C       +       S S+S     +CP
Sbjct: 296 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYK-IQCP 355

Query: 324 VCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITLRHLAQ-----NKRWRRCQECRR 383
            CQ   C +C  PWH  ++C+E++          D  LRH A       +  ++C +C+ 
Sbjct: 356 TCQLIWCFKCHSPWHEGVNCKEYKK--------GDKLLRHWASEIEHGQRNAQKCPKCKI 415

Query: 384 MIELTQGCYHMTC-WCGHEFCYSCGGEYQ 406
            I+ T+GC HMTC  C   FCY CG  Y+
Sbjct: 416 HIQRTEGCDHMTCSQCNTNFCYRCGERYR 430

BLAST of CmoCh13G004710 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-20
Identity = 66/209 (31.58%), Postives = 94/209 (44.98%), Query Frame = 0

Query: 204 CSICCDNKPSAMIVALKCCHK-FCSNCLKTYVDGKVEASQVPIRCPQLRCQYYITNNEFR 263
           C +C ++KP   I  L CC K  C  CLK Y+  +V+  QV I+CP   C  ++   E  
Sbjct: 263 CRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFL--EETT 322

Query: 264 SFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECP 323
               LT  +S+         R D    P P C       +       S S+S     +CP
Sbjct: 323 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYK-IQCP 382

Query: 324 VCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITLRHLAQ-----NKRWRRCQECRR 383
            CQ   C +C  PWH  ++C+E++          D  LRH A       +  ++C +C+ 
Sbjct: 383 TCQFVWCFKCHSPWHEGVNCKEYKK--------GDKLLRHWASEIEHGQRNAQKCPKCKI 442

Query: 384 MIELTQGCYHMTC-WCGHEFCYSCGGEYQ 406
            I+ T+GC HMTC  C   FCY CG  Y+
Sbjct: 443 HIQRTEGCDHMTCSQCNTNFCYRCGERYR 457

BLAST of CmoCh13G004710 vs. ExPASy Swiss-Prot
Match: Q4KLT0 (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-18
Identity = 61/212 (28.77%), Postives = 92/212 (43.40%), Query Frame = 0

Query: 203 NCSICCDNKPSAMIVALKCCHK-FCSNCLKTYVDGKVEASQVPIRCPQLRCQYYITNNEF 262
           +C +C +++    I  L CC K  C  CLK Y+  +V+  Q  I+CP   C  ++  +  
Sbjct: 2   SCRVCLEDR---SIKPLPCCKKPVCDECLKRYLSSQVQLGQAEIQCPITECNKHLDESTI 61

Query: 263 RSFLPLTLYESLDNTLAEANL-RSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSC-F 322
              LP   ++ +        L R D    P P C               + ++S+N    
Sbjct: 62  LYSLP---HDDIIKYKYFLELSRMDSSTKPCPQCKHFTTFKR--KTHIPNPTKSENKLKI 121

Query: 323 ECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITLRHLAQ-----NKRWRRCQE 382
           +CP CQ   C  C  PWH  ++C E++          D  LRH A       +  ++C  
Sbjct: 122 QCPSCQFIWCFRCHAPWHEGVNCREYKK--------GDKLLRHWANEIEHGQRNAQKCPR 181

Query: 383 CRRMIELTQGCYHMTC-WCGHEFCYSCGGEYQ 406
           C+  I+ T+GC HMTC  C   FCY CG  Y+
Sbjct: 182 CKVHIQRTEGCDHMTCSQCNTNFCYRCGERYR 197

BLAST of CmoCh13G004710 vs. ExPASy Swiss-Prot
Match: Q9SKC3 (Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI9 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 8.4e-17
Identity = 65/263 (24.71%), Postives = 103/263 (39.16%), Query Frame = 0

Query: 187 AIGVLSRP----------KKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDG 246
           A+G+L  P          KK  K    C IC ++     I  + C H +C  C   Y+  
Sbjct: 101 AVGLLKEPVVDFNGGEKDKKCRKVNIQCGICFESYTREEIARVSCGHPYCKTCWAGYITT 160

Query: 247 KVEASQ--VPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDR--IYCPFP 306
           K+E     + ++CP+  C   +  +         + E     +  + +   +   +CP P
Sbjct: 161 KIEDGPGCLRVKCPEPSCSAAVGKDMIEDVTETKVNEKYSRYILRSYVEDGKKIKWCPSP 220

Query: 307 NCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEE 366
            C   ++         S SS  D SC    +C    C  C    HS + C+       + 
Sbjct: 221 GCGYAVE------FGGSESSSYDVSC----LCSYRFCWNCSEDAHSPVDCDTVSKWIFKN 280

Query: 367 RDTADITLRHLAQNKRW-----RRCQECRRMIELTQGCYHMTCW--CGHEFCYSCGGEYQ 426
           +D         ++NK W     + C EC+R IE   GC HMTC   CGHEFC+ C   Y+
Sbjct: 281 QDE--------SENKNWMLANSKPCPECKRPIEKNDGCNHMTCSAPCGHEFCWICLKAYR 340

Query: 427 DGQQTCRCTLWDEENSEDMVTHS 429
                C   + ++  S+  +  S
Sbjct: 341 RHSGACNRFVVEQAESKRALLQS 345

BLAST of CmoCh13G004710 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.6e-15
Identity = 67/282 (23.76%), Postives = 116/282 (41.13%), Query Frame = 0

Query: 203 NCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQ--VPIRCPQLRCQYYITNNE 262
           +C IC ++ P     AL C H++C  C K Y++ KV      +   CP  +C+  +  + 
Sbjct: 138 SCLICLEDYPPTQTFALICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 197

Query: 263 FRSFLPLTLYESLDNTLAEANLRSDR--IYCPFPNCSVLLDPSECLSARASSSSQSDNSC 322
           F+  +   ++E  +N + ++ +  +    +CP P C          S R     + +   
Sbjct: 198 FKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGC--------IYSIRCDRKERKEAVN 257

Query: 323 FECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITLRHLAQNKRWRRCQECRRM 382
            +C     F C + ++  H    C +      +  D ++     LA  K   +C ECR  
Sbjct: 258 CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCPECRSP 317

Query: 383 IELTQGCYHMTC-----WCGHEFCYSCGGEYQDGQQTC----RCTLWDEENS--EDMVTH 442
           IE   GC HMTC      CG EFC+ C G + +   T      C  +D+  +  +D   H
Sbjct: 318 IEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNCNKYDKSKAKEDDDKAH 377

Query: 443 SIQ---ESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLA 467
             +   E+  + +  + S    M    EQ R+     Q+ L+
Sbjct: 378 DAKTELEAYMFYYHRYESHRNAMKIADEQRRNAHLKEQQILS 408

BLAST of CmoCh13G004710 vs. ExPASy TrEMBL
Match: A0A6J1EGQ6 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434167 PE=3 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 2.9e-302
Identity = 503/503 (100.00%), Postives = 503/503 (100.00%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCTDSSYVDTMKDIHQLPWLER
Sbjct: 481 PRCTDSSYVDTMKDIHQLPWLER 503

BLAST of CmoCh13G004710 vs. ExPASy TrEMBL
Match: A0A6J1KEV2 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495184 PE=3 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.5e-287
Identity = 478/503 (95.03%), Postives = 489/503 (97.22%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREI GRGSSRVLAE+DDGEDFRSCCED EVWK+NEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREISGRGSSRVLAEEDDGEDFRSCCEDQEVWKENEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGLSGIGVFME SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMENSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEE+PEIPLL+ALKERILEHASNFEAFSLNLSS+T+LEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEEPEIPLLVALKERILEHASNFEAFSLNLSSNTELEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVE+
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVES 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
            QVPIRCPQLRC+YYITNNEFRSFLPLTLYESL+NTLAEAN R DRIYCPFPNCSVLLDP
Sbjct: 241 FQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANFRLDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQ VPLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQKVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCG+EFCYSCG EYQDGQQTC C LWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGYEFCYSCGAEYQDGQQTCECALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE +VTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEYLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCTDSS VDTMKDIHQLPWLER
Sbjct: 481 PRCTDSSDVDTMKDIHQLPWLER 503

BLAST of CmoCh13G004710 vs. ExPASy TrEMBL
Match: A0A6J1EKK2 (probable E3 ubiquitin-protein ligase rbrA isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434167 PE=3 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 7.3e-282
Identity = 475/503 (94.43%), Postives = 475/503 (94.43%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGLSGIGVFMEKSGKVPFI                            Q
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFI----------------------------Q 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 475

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCTDSSYVDTMKDIHQLPWLER
Sbjct: 481 PRCTDSSYVDTMKDIHQLPWLER 475

BLAST of CmoCh13G004710 vs. ExPASy TrEMBL
Match: A0A1S3C9E4 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.9e-268
Identity = 445/503 (88.47%), Postives = 475/503 (94.43%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           M+HR+IFGRGSS   AE+DD E+FRSCCEDHEVWK+ E A EE+S  EEDLDEFSVK+FF
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES--EEDLDEFSVKMFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIA IGDPASGLSGIGVFMEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           ND+  IRAFTDSD LYKQITNEE+PEIPLL+ALKERILEHASNFE+FSL LSSS DLEQP
Sbjct: 121 NDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLS P+KGDKSVENCSICCDNKPSAM+VALKCCHKFCS CLKTYVDGKV+A
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYIT+NEF+SFLPLTLYE L+NTLAEAN+ +DRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSC ECPVCQRFICVECQVPWHSS+SCEEFQN+PLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
             LAQNKRWRRCQEC RMIELTQGC+HMTCWCGHEFCYSCG EYQDGQQTC+C  WDE+N
Sbjct: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE++VTHS+QESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCTD SYVDTMKDIHQLPWLER
Sbjct: 481 PRCTD-SYVDTMKDIHQLPWLER 500

BLAST of CmoCh13G004710 vs. ExPASy TrEMBL
Match: A0A5D3E1X1 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G001170 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.9e-268
Identity = 445/503 (88.47%), Postives = 475/503 (94.43%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           M+HR+IFGRGSS   AE+DD E+FRSCCEDHEVWK+ E A EE+S  EEDLDEFSVK+FF
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES--EEDLDEFSVKMFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIA IGDPASGLSGIGVFMEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           ND+  IRAFTDSD LYKQITNEE+PEIPLL+ALKERILEHASNFE+FSL LSSS DLEQP
Sbjct: 121 NDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLS P+KGDKSVENCSICCDNKPSAM+VALKCCHKFCS CLKTYVDGKV+A
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYIT+NEF+SFLPLTLYE L+NTLAEAN+ +DRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSC ECPVCQRFICVECQVPWHSS+SCEEFQN+PLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
             LAQNKRWRRCQEC RMIELTQGC+HMTCWCGHEFCYSCG EYQDGQQTC+C  WDE+N
Sbjct: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE++VTHS+QESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCTD SYVDTMKDIHQLPWLER
Sbjct: 481 PRCTD-SYVDTMKDIHQLPWLER 500

BLAST of CmoCh13G004710 vs. NCBI nr
Match: KAG6583802.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 542/558 (97.13%), Postives = 549/558 (98.39%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAE+DDGEDFRSCCED EVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEEQPEIPLL+ALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYITNNEFRSFLPLTLYESL+NTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRC LWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLERGKVKEFLTLVFVLHLHRRAWRFDVLNHAAAAAYDVAL 540
           PRCT+SSYVDTMKDIHQLPWLERGKVKEFLTLVFVLHLHRR WRFDV+NHAAAAAYDVAL
Sbjct: 481 PRCTESSYVDTMKDIHQLPWLERGKVKEFLTLVFVLHLHRRTWRFDVVNHAAAAAYDVAL 540

Query: 541 RGPPCIRFEWGNLMQTNA 559
           RGPPCIR EW NLMQTNA
Sbjct: 541 RGPPCIRLEWRNLMQTNA 558

BLAST of CmoCh13G004710 vs. NCBI nr
Match: XP_022927282.1 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1047.0 bits (2706), Expect = 5.9e-302
Identity = 503/503 (100.00%), Postives = 503/503 (100.00%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCTDSSYVDTMKDIHQLPWLER
Sbjct: 481 PRCTDSSYVDTMKDIHQLPWLER 503

BLAST of CmoCh13G004710 vs. NCBI nr
Match: KAG7019427.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1026.9 bits (2654), Expect = 6.3e-296
Identity = 491/503 (97.61%), Postives = 497/503 (98.81%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAE+DDGEDFRSCCED EVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEEQPEIPLL+ALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYITNNEFRSFLPLTLYESL+NTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRC LWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCT+SSYVDTMKDIHQLPWLER
Sbjct: 481 PRCTESSYVDTMKDIHQLPWLER 503

BLAST of CmoCh13G004710 vs. NCBI nr
Match: XP_023519477.1 (E3 ubiquitin-protein ligase arih1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.3e-293
Identity = 488/503 (97.02%), Postives = 495/503 (98.41%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDH EIFGRGSSRVLAE+DDGEDFRSCCEDHEVWK+NEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHPEIFGRGSSRVLAEEDDGEDFRSCCEDHEVWKENEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGLSGI VFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGICVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEEQPEIPLL+ALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLLALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYITN EFRSFLPLTLYESL+NTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITNTEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
            HLAQNKRWRRC+ECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRC LWDEEN
Sbjct: 361 HHLAQNKRWRRCEECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           P+CTDSSYVDTMKDIHQLPWLER
Sbjct: 481 PQCTDSSYVDTMKDIHQLPWLER 503

BLAST of CmoCh13G004710 vs. NCBI nr
Match: XP_023000867.1 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 998.0 bits (2579), Expect = 3.2e-287
Identity = 478/503 (95.03%), Postives = 489/503 (97.22%), Query Frame = 0

Query: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREI GRGSSRVLAE+DDGEDFRSCCED EVWK+NEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREISGRGSSRVLAEEDDGEDFRSCCEDQEVWKENEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGLSGIGVFME SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMENSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEE+PEIPLL+ALKERILEHASNFEAFSLNLSS+T+LEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEEPEIPLLVALKERILEHASNFEAFSLNLSSNTELEQP 180

Query: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVE+
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVES 240

Query: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300
            QVPIRCPQLRC+YYITNNEFRSFLPLTLYESL+NTLAEAN R DRIYCPFPNCSVLLDP
Sbjct: 241 FQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANFRLDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQ VPLEERDTADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQKVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCG+EFCYSCG EYQDGQQTC C LWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGYEFCYSCGAEYQDGQQTCECALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE +VTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEYLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTDSSYVDTMKDIHQLPWLER 504
           PRCTDSS VDTMKDIHQLPWLER
Sbjct: 481 PRCTDSSDVDTMKDIHQLPWLER 503

BLAST of CmoCh13G004710 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 612.8 bits (1579), Expect = 2.7e-175
Identity = 307/501 (61.28%), Postives = 387/501 (77.25%), Query Frame = 0

Query: 20  DGEDFRSCCEDHEVW--KDNEFAGEEQSEKEEDL-DEFSVKLFFKGVSIAGIGDPASGLS 79
           D E+FRSCC D E+W    ++ A   + EK++DL DEFSVK+ FKGVSI   GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIHSIRAFTDSDPLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++ S+ A TDS+ LY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 KQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPKK 199
            QIT EEQ EIPLL+AL+ER+LE  SN   F L L+   DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCQYYI 259
           GDK +ENCSICC+++ S M+++LKC HKFCS+C+KTYV+GKV+ S+VPIRCPQ++C++Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 TNNEFRSFLPLTLYESLDNTLAEANL---RSDRIYCPFPNCSVLLDPSECLS---ARASS 319
           +  E +SFLP+T ++S +    EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITLRHLAQNKRW 379
           S+QS+NS C ECPVC+RF+CV+C VPWH+SMSCEEFQ +P++ER   DITL  LA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEENSED---MVT 439
           RRCQ+CR MIEL QGC HMTC CGHEFCYSCG EY++GQQTC C  WD++  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MD YSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TDSSYVD-TMKDIHQLPWLER 504
           T+SSYV+  MKD+HQLPWLER
Sbjct: 500 TESSYVEAAMKDLHQLPWLER 516

BLAST of CmoCh13G004710 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 612.8 bits (1579), Expect = 2.7e-175
Identity = 307/501 (61.28%), Postives = 387/501 (77.25%), Query Frame = 0

Query: 20  DGEDFRSCCEDHEVW--KDNEFAGEEQSEKEEDL-DEFSVKLFFKGVSIAGIGDPASGLS 79
           D E+FRSCC D E+W    ++ A   + EK++DL DEFSVK+ FKGVSI   GD  SG S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIHSIRAFTDSDPLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++ S+ A TDS+ LY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 KQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPKK 199
            QIT EEQ EIPLL+AL+ER+LE  SN   F L L+   DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCQYYI 259
           GDK +ENCSICC+++ S M+++LKC HKFCS+C+KTYV+GKV+ S+VPIRCPQ++C++Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 TNNEFRSFLPLTLYESLDNTLAEANL---RSDRIYCPFPNCSVLLDPSECLS---ARASS 319
           +  E +SFLP+T ++S +    EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITLRHLAQNKRW 379
           S+QS+NS C ECPVC+RF+CV+C VPWH+SMSCEEFQ +P++ER   DITL  LA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEENSED---MVT 439
           RRCQ+CR MIEL QGC HMTC CGHEFCYSCG EY++GQQTC C  WD++  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MD YSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TDSSYVD-TMKDIHQLPWLER 504
           T+SSYV+  MKD+HQLPWLER
Sbjct: 500 TESSYVEAAMKDLHQLPWLER 516

BLAST of CmoCh13G004710 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 194.5 bits (493), Expect = 2.3e-49
Identity = 123/379 (32.45%), Postives = 199/379 (52.51%), Query Frame = 0

Query: 57  KLFFKGV--SIAGIGDPASGLSGIGV-FMEKSGKVPFIQVQKKLDFYVDELVADYLALMD 116
           +L+FKG+     G G     +SG GV   ++   + F      +D  +    A+  AL+ 
Sbjct: 146 RLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPLIDNGMSRQGAELKALIR 205

Query: 117 GLVEAMQNDIHSIRAFTDSDPLYKQITNE---EQPEIPLLIALKERILEHASNFEAFSLN 176
           GL EA++  I  I  F DS P+++ +T +   +Q +I LL+   + I++H S+++     
Sbjct: 206 GLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQHV--- 265

Query: 177 LSSSTDLEQPFHLAKVAIGVLSRPKKGDKSV-----ENCSICC-DNKPSAMIVALKCCHK 236
           L +  D++  + LA+ +I  +  P +  +       E C+IC  D     M    KC H+
Sbjct: 266 LVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHR 325

Query: 237 FCSNCLKTYVDGKVEASQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLR- 296
           FC  C+K +V+ K+     P +CP   C+  +  +     L   L +     L E  +  
Sbjct: 326 FCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPV 385

Query: 297 SDRIYCPFPNCSVLLDPSECL-SARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSC 356
           ++R+YCP+P CS L+  ++   SA++  S    +    C  C+   CV+C+VPWH ++SC
Sbjct: 386 TERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSC 445

Query: 357 EEFQNVPLEERDTADITLRHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGG 416
            E++ +   E    D+ L+ LA NK WR+C +C+ MIEL+QGC H+TC CGHEFCY+CGG
Sbjct: 446 TEYKKLH-PEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGG 505

Query: 417 EYQDGQQTC--RCTLWDEE 420
            +     TC  RC  W+EE
Sbjct: 506 GWNKIMGTCLNRCPTWNEE 519

BLAST of CmoCh13G004710 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 8.6e-41
Identity = 104/311 (33.44%), Postives = 167/311 (53.70%), Query Frame = 0

Query: 109 LALMDGLVEAMQNDIHSIRAFTDSDPLYKQIT--NEEQPEIPLLIALKERILEHASNFEA 168
           +AL+ GL E+    I  +  + D   LY+ I    + + ++  L+   + +LE  +  +A
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKKKVVHLVDEFQLLLEEMTYTDA 60

Query: 169 FSLNLSSSTDLEQPFHLAKVAIGVLSRPKKGDKSVENCSICCDNKPS--AMIVALKCCHK 228
              +L +  D+   F LA+ AI       + D   E CSIC +            KC H+
Sbjct: 61  ---DLIALNDVNFAFKLAREAI-----VSRDDVKAEICSICFEETEGERMFFTTEKCVHR 120

Query: 229 FCSNCLKTYVDGKVEASQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANL-R 288
            C  C+K YV+ K+ +  VP  C    C++ +T       L L L E     + E ++  
Sbjct: 121 HCFPCVKQYVEVKLLSGTVP-TCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPA 180

Query: 289 SDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCE 348
           ++RIYCP+PNCS+L+  +E  S    S   +D SC +C  C  F C++C+VP HS +SC 
Sbjct: 181 AERIYCPYPNCSMLMSKTELSS---ESDLSNDRSCVKC--CGLF-CIDCKVPSHSDLSCA 240

Query: 349 EFQNV---PLEERDTADITLRHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSC 408
           E++ +   PL +    ++ L+ LA++K+WR+C+ CR MIEL+  C HMTC CG++FCY C
Sbjct: 241 EYKKLHHDPLVD----ELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQC 292

Query: 409 GGEYQDGQQTC 412
             E+++ Q+TC
Sbjct: 301 EVEWKNDQKTC 292

BLAST of CmoCh13G004710 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 4.3e-40
Identity = 118/377 (31.30%), Postives = 194/377 (51.46%), Query Frame = 0

Query: 41  GEEQSEKEEDLDEFSVKLFFKG-VSIAGIGDPASGL----SGIGVFMEKSGKVPFIQVQK 100
           GE  S   + +D    +L FKG V+   + D          G+ +  EK   +  I+ + 
Sbjct: 139 GEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIK-ES 198

Query: 101 KLDFYVDELVADYLALMDGLVEAMQNDIHSIRAFTDSDPLYKQI--TNEEQPEIPLLIAL 160
             D  +     + +AL+ GL E+    + ++  + D D +Y+ I    + + +I  L+  
Sbjct: 199 LRDIEISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKKKIDHLVEE 258

Query: 161 KERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPKKGDKSVENCSICCDNKP- 220
            + ILE  +  +A    L +  D++  F LA+ AIG  S     ++  E C I  +    
Sbjct: 259 VQGILEKMACIDAV---LVARNDVKFAFRLAREAIGRNSVDVNAEQG-ETCGIFFEETDV 318

Query: 221 SAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCQYYITNNEFRSFLPLTLYES 280
             M V  KC H+ C  C+K +V  K+ +   P  C +  C++ +T       L L L E 
Sbjct: 319 EHMFVTEKCLHRHCFPCVKQHVKVKLRSGTEP-TCLEYGCKFKLTLERCSKVLTLKLIEM 378

Query: 281 LDNTLAEANL-RSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICVE 340
               + E ++  ++RIYCP+PNCS+L+  +E LS+ A  S+    +C +C  C  F C++
Sbjct: 379 WKQKMKEDSIPAAERIYCPYPNCSMLMSKTE-LSSEADLSNV--RTCVKC--CGLF-CID 438

Query: 341 CQVPWHSSMSCEEFQNV---PLEERDTADITLRHLAQNKRWRRCQECRRMIELTQGCYHM 400
           C+VP H+ +S ++++ +   PL +    D+ L+ LA +K WR+C +CR MIEL+ GC HM
Sbjct: 439 CKVPSHTDLSYDDYKKLHPDPLVD----DLKLKSLANDKMWRQCVKCRHMIELSHGCNHM 498

Query: 401 TCWCGHEFCYSCGGEYQ 406
           TC CG+EFCY CG E+Q
Sbjct: 499 TCRCGYEFCYECGIEWQ 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
D3YYI79.6e-2131.58Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P... [more]
Q8TC411.3e-2031.58Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE... [more]
Q4KLT01.5e-1828.77Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 ... [more]
Q9SKC38.4e-1724.71Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q6T4861.6e-1523.76Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
Match NameE-valueIdentityDescription
A0A6J1EGQ62.9e-302100.00ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita mo... [more]
A0A6J1KEV21.5e-28795.03ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita ma... [more]
A0A6J1EKK27.3e-28294.43probable E3 ubiquitin-protein ligase rbrA isoform X2 OS=Cucurbita moschata OX=36... [more]
A0A1S3C9E47.9e-26888.47E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 S... [more]
A0A5D3E1X17.9e-26888.47E3 ubiquitin-protein ligase arih1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
KAG6583802.10.0e+0097.13putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022927282.15.9e-302100.00ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita mosc... [more]
KAG7019427.16.3e-29697.61putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. arg... [more]
XP_023519477.11.3e-29397.02E3 ubiquitin-protein ligase arih1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023000867.13.2e-28795.03ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita maxi... [more]
Match NameE-valueIdentityDescription
AT4G19670.12.7e-17561.28RING/U-box superfamily protein [more]
AT4G19670.22.7e-17561.28RING/U-box superfamily protein [more]
AT5G60250.12.3e-4932.45zinc finger (C3HC4-type RING finger) family protein [more]
AT2G25360.18.6e-4133.44RING/U-box superfamily protein [more]
AT2G25370.14.3e-4031.30RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 352..411
e-value: 0.057
score: 15.6
coord: 271..342
e-value: 2.2E-7
score: 40.5
IPR002867IBR domainPFAMPF01485IBRcoord: 362..411
e-value: 3.7E-6
score: 27.0
coord: 282..342
e-value: 2.3E-8
score: 34.1
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 204..250
e-value: 0.0012
score: 28.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 204..251
score: 10.836942
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 198..283
e-value: 3.5E-14
score: 54.5
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 74..170
e-value: 1.0E-9
score: 38.3
NoneNo IPR availableGENE3D1.20.120.1750coord: 339..465
e-value: 2.0E-15
score: 59.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR11685:SF353RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 18..503
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 199..275
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 355..419
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 56..189
e-value: 1.2E-6
score: 30.4
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 18..503
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 221..230
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 200..417
score: 27.908644

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G004710.1CmoCh13G004710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0004386 helicase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity