CmoCh13G002220 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G002220
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4-like
LocationCmo_Chr13: 1748704 .. 1750389 (+)
RNA-Seq ExpressionCmoCh13G002220
SyntenyCmoCh13G002220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCGGCGCTGGTTCAACCTACATTTTTGGAACATACTCGAAAGCAATAAAGTCGCAATTTGATTACAATCAAACACAAATCAACACATTAGGATTTGCCAAAGATCTCGGCTCAAACTTCGGAGTTTTCGCTGGCCTTCTCGGCGAAGTTGCTCCACCATGGGTTCTATTCCTCGTCGGCTCAGCTCTCAACTTCTGGAGCTACTTCATGATTTGGCTCTCCATCACCGGCCGTATCTCCAAACCCCCGTTTTGGGAAATGTTCGTTTTCATCTGCGTAGCTGCGAATTCACAAAATTTCGCCAACACCGCCATTTTGGTCACGAGCGTTCGAAACTTCCCGGACCGACGAGGCATCATTCTTGGGCTTCTCAAGGGTTTCGTTGGGTTCGGAGGTGCTATTTTGACTCAATTCTATTTAGCTCTTTACGGCCATGATAACCCAACCAATTTGGTGCTTCTTCTCTCTTGGCTACCAACCGTTGCGTCTATGTTGTTTTTTCTCTCAATTCGACCAATCAAAGTCCGTAAAGATCCAGATGAGCTTAGAGTTCTTTACCATTTGCTTTATGTGTCCATAATTTTGGCGCTGTTTCTCTTATTCTTAACCATAACCCAAAAACAAACAGTGTTTACTCAAGAAGCTTACATGAGCGGCGCCACCGTGATCATCGGCTTATTGCTTATCCCTCTTTTGATAGCCATTAGAGAAGAGTTCATGCTCTTCAAACTCAACAAACAAACCCTAAATAACAATCCTACCGTCCCCATTTTAATGCCGGAGCACGAGCCTTCCCCCAAAGCCCCACCCACAATACAGCAAATTCCCGAAGCTTCAACCTCGTGTTTCTCAATGACATTCAAAAAGCCACAAAGAGGTGAAGATTTCACCATTTTACAAGCTTTATTCAGCAAAGACATGGCGCTTGTTTTCATAGCCACACTTACTGCCTGTGGCGCATCAATTGCAGCCATTGATAACTTAGGTCAGGTTGGTGAATCACTTGGCTATCCATATCAAACAATAAGCATATTCGTTTCGTGGGTTTCGATATTCAATTTCTTCGGTCGGGTCTTCTCCGGTTTCATCTCGGAAGCGCTTATGACCAAATACAAATTACCCCGACCTCTCATGTTCGCTTTTTCCCATCTTTTAACTTGCATTGGCATTCTCTTTATTGCCTTCCCATATAAGGGTTCCGTTTATGCAGCGTCCTTGATCATTGGGTTCGGGTTTGGAGCGCAAGTTCCAATGTTGTTCGCCATAATCTCGGAACTATTCGGCCTCAAATATTATTCGACAATCTTCAATTGTGGGCAATTGGCTGTCCCAATTGGATCTTACATACTGAACGTTGATGTAATTGGGAAGTTGTATGACATTGAAGCAGCAAAGCTTGGTGGGGTTAAGAATGGGAAGGGGCTAACTTGCACGGGCACTCATTGCTTTAGTGGGTCTTTCTTGATTTTGGCTGTGGTGGTATTGTTCGGGGCGGTGGCTTCGCTTGTGTTGGCTTATAGGACTCGAGACTTTTACAAAGGCGACGTTTATAACAGGTATAGAGAGGACATGTGGATTCCTCAATCGGATATGGAATTCTATTGTATAGATGACAGGAAGAAAGGTGATGACAATTTAGCACGGATGGATATGCCGCCCAAGTATAATTTTCTCAAGTCTTAA

mRNA sequence

ATGATCGGCGCTGGTTCAACCTACATTTTTGGAACATACTCGAAAGCAATAAAGTCGCAATTTGATTACAATCAAACACAAATCAACACATTAGGATTTGCCAAAGATCTCGGCTCAAACTTCGGAGTTTTCGCTGGCCTTCTCGGCGAAGTTGCTCCACCATGGGTTCTATTCCTCGTCGGCTCAGCTCTCAACTTCTGGAGCTACTTCATGATTTGGCTCTCCATCACCGGCCGTATCTCCAAACCCCCGTTTTGGGAAATGTTCGTTTTCATCTGCGTAGCTGCGAATTCACAAAATTTCGCCAACACCGCCATTTTGGTCACGAGCGTTCGAAACTTCCCGGACCGACGAGGCATCATTCTTGGGCTTCTCAAGGGTTTCGTTGGGTTCGGAGGTGCTATTTTGACTCAATTCTATTTAGCTCTTTACGGCCATGATAACCCAACCAATTTGGTGCTTCTTCTCTCTTGGCTACCAACCGTTGCGTCTATGTTGTTTTTTCTCTCAATTCGACCAATCAAAGTCCGTAAAGATCCAGATGAGCTTAGAGTTCTTTACCATTTGCTTTATGTGTCCATAATTTTGGCGCTGTTTCTCTTATTCTTAACCATAACCCAAAAACAAACAGTGTTTACTCAAGAAGCTTACATGAGCGGCGCCACCGTGATCATCGGCTTATTGCTTATCCCTCTTTTGATAGCCATTAGAGAAGAGTTCATGCTCTTCAAACTCAACAAACAAACCCTAAATAACAATCCTACCGTCCCCATTTTAATGCCGGAGCACGAGCCTTCCCCCAAAGCCCCACCCACAATACAGCAAATTCCCGAAGCTTCAACCTCGTGTTTCTCAATGACATTCAAAAAGCCACAAAGAGGTGAAGATTTCACCATTTTACAAGCTTTATTCAGCAAAGACATGGCGCTTGTTTTCATAGCCACACTTACTGCCTGTGGCGCATCAATTGCAGCCATTGATAACTTAGGTCAGGTTGGTGAATCACTTGGCTATCCATATCAAACAATAAGCATATTCGTTTCGTGGGTTTCGATATTCAATTTCTTCGGTCGGGTCTTCTCCGGTTTCATCTCGGAAGCGCTTATGACCAAATACAAATTACCCCGACCTCTCATGTTCGCTTTTTCCCATCTTTTAACTTGCATTGGCATTCTCTTTATTGCCTTCCCATATAAGGGTTCCGTTTATGCAGCGTCCTTGATCATTGGGTTCGGGTTTGGAGCGCAAGTTCCAATGTTGTTCGCCATAATCTCGGAACTATTCGGCCTCAAATATTATTCGACAATCTTCAATTGTGGGCAATTGGCTGTCCCAATTGGATCTTACATACTGAACGTTGATGTAATTGGGAAGTTGTATGACATTGAAGCAGCAAAGCTTGGTGGGGTTAAGAATGGGAAGGGGCTAACTTGCACGGGCACTCATTGCTTTAGTGGGTCTTTCTTGATTTTGGCTGTGGTGGTATTGTTCGGGGCGGTGGCTTCGCTTGTGTTGGCTTATAGGACTCGAGACTTTTACAAAGGCGACGTTTATAACAGGTATAGAGAGGACATGTGGATTCCTCAATCGGATATGGAATTCTATTGTATAGATGACAGGAAGAAAGGTGATGACAATTTAGCACGGATGGATATGCCGCCCAAGTATAATTTTCTCAAGTCTTAA

Coding sequence (CDS)

ATGATCGGCGCTGGTTCAACCTACATTTTTGGAACATACTCGAAAGCAATAAAGTCGCAATTTGATTACAATCAAACACAAATCAACACATTAGGATTTGCCAAAGATCTCGGCTCAAACTTCGGAGTTTTCGCTGGCCTTCTCGGCGAAGTTGCTCCACCATGGGTTCTATTCCTCGTCGGCTCAGCTCTCAACTTCTGGAGCTACTTCATGATTTGGCTCTCCATCACCGGCCGTATCTCCAAACCCCCGTTTTGGGAAATGTTCGTTTTCATCTGCGTAGCTGCGAATTCACAAAATTTCGCCAACACCGCCATTTTGGTCACGAGCGTTCGAAACTTCCCGGACCGACGAGGCATCATTCTTGGGCTTCTCAAGGGTTTCGTTGGGTTCGGAGGTGCTATTTTGACTCAATTCTATTTAGCTCTTTACGGCCATGATAACCCAACCAATTTGGTGCTTCTTCTCTCTTGGCTACCAACCGTTGCGTCTATGTTGTTTTTTCTCTCAATTCGACCAATCAAAGTCCGTAAAGATCCAGATGAGCTTAGAGTTCTTTACCATTTGCTTTATGTGTCCATAATTTTGGCGCTGTTTCTCTTATTCTTAACCATAACCCAAAAACAAACAGTGTTTACTCAAGAAGCTTACATGAGCGGCGCCACCGTGATCATCGGCTTATTGCTTATCCCTCTTTTGATAGCCATTAGAGAAGAGTTCATGCTCTTCAAACTCAACAAACAAACCCTAAATAACAATCCTACCGTCCCCATTTTAATGCCGGAGCACGAGCCTTCCCCCAAAGCCCCACCCACAATACAGCAAATTCCCGAAGCTTCAACCTCGTGTTTCTCAATGACATTCAAAAAGCCACAAAGAGGTGAAGATTTCACCATTTTACAAGCTTTATTCAGCAAAGACATGGCGCTTGTTTTCATAGCCACACTTACTGCCTGTGGCGCATCAATTGCAGCCATTGATAACTTAGGTCAGGTTGGTGAATCACTTGGCTATCCATATCAAACAATAAGCATATTCGTTTCGTGGGTTTCGATATTCAATTTCTTCGGTCGGGTCTTCTCCGGTTTCATCTCGGAAGCGCTTATGACCAAATACAAATTACCCCGACCTCTCATGTTCGCTTTTTCCCATCTTTTAACTTGCATTGGCATTCTCTTTATTGCCTTCCCATATAAGGGTTCCGTTTATGCAGCGTCCTTGATCATTGGGTTCGGGTTTGGAGCGCAAGTTCCAATGTTGTTCGCCATAATCTCGGAACTATTCGGCCTCAAATATTATTCGACAATCTTCAATTGTGGGCAATTGGCTGTCCCAATTGGATCTTACATACTGAACGTTGATGTAATTGGGAAGTTGTATGACATTGAAGCAGCAAAGCTTGGTGGGGTTAAGAATGGGAAGGGGCTAACTTGCACGGGCACTCATTGCTTTAGTGGGTCTTTCTTGATTTTGGCTGTGGTGGTATTGTTCGGGGCGGTGGCTTCGCTTGTGTTGGCTTATAGGACTCGAGACTTTTACAAAGGCGACGTTTATAACAGGTATAGAGAGGACATGTGGATTCCTCAATCGGATATGGAATTCTATTGTATAGATGACAGGAAGAAAGGTGATGACAATTTAGCACGGATGGATATGCCGCCCAAGTATAATTTTCTCAAGTCTTAA

Protein sequence

MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDRKKGDDNLARMDMPPKYNFLKS
Homology
BLAST of CmoCh13G002220 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 6.9e-27
Identity = 142/541 (26.25%), Postives = 242/541 (44.73%), Query Frame = 0

Query: 5   GSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLVGSAL 64
           G+ + F  YS  +KS    +Q ++N L  A DLG  FG  +G+     P  V+    +A+
Sbjct: 58  GTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAAAM 117

Query: 65  NFWSYFMIWLSITGRISKPPFWEMFVFIC--VAANSQNFANTAILVTSVRNFPDRRGIIL 124
            F  Y + WL IT  I+ P      VF+C  +A  S  + NTA  +  +R+FP+ R + L
Sbjct: 118 GFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICWFNTACFILCIRHFPNNRALAL 177

Query: 125 GLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLL-SWLPTVASM--LFFLSIRP-IKVRK 184
            L   F G   A+ +  + A+  + + +NL LLL S +P V S   L+ +  +P +    
Sbjct: 178 SLTVSFNGISAALYSLAFNAI--NPSSSNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTP 237

Query: 185 DPDELRVLYHLLYVSIILALFLLF-LTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIR 244
           D D  R   H+  +  +LA+   F L ++   T   +  ++    +++  L  PLL+  R
Sbjct: 238 DYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLLVFPLCAPLLVYAR 297

Query: 245 EEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMT---------- 304
           + F+            P +   +  HE S      I ++    TS  S T          
Sbjct: 298 DYFL------------PVINARL-NHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKE 357

Query: 305 FKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAI--DNLGQVGESLGYPYQTISI 364
               + G++ +    +   +  L +IA    CG +I  +  +NLGQ+ +SLG   Q  + 
Sbjct: 358 GNTVRLGDEHSFRLLISRLEFWLYYIAYF--CGGTIGLVYSNNLGQIAQSLG---QNSTT 417

Query: 365 FVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIG--ILFIAFPYKGSVY 424
            V+  S F+FFGR+ S    + +  +++L R   FA + L T I   +L ++   + ++ 
Sbjct: 418 LVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQ 477

Query: 425 AASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIE 484
            A+ +IG   G       +I S+LFG        N     +PIGS +    +   +Y+  
Sbjct: 478 TATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYEAN 537

Query: 485 AAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYRE 525
           A+          + C G  C+  +F+    + + G V+SL L  RT+      VY+R  +
Sbjct: 538 ASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTK-----PVYHRLEQ 568

BLAST of CmoCh13G002220 vs. ExPASy TrEMBL
Match: A0A6J1HIM6 (uncharacterized protein LOC111464777 OS=Cucurbita moschata OX=3662 GN=LOC111464777 PE=4 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR
Sbjct: 511 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLKS 562
           KKGDDNLARMDMPPKYNFLKS
Sbjct: 571 KKGDDNLARMDMPPKYNFLKS 591

BLAST of CmoCh13G002220 vs. ExPASy TrEMBL
Match: A0A6J1HIM2 (uncharacterized protein LOC111464772 OS=Cucurbita moschata OX=3662 GN=LOC111464772 PE=4 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 549/560 (98.04%), Postives = 555/560 (99.11%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRI+KPPFWEMF+FICVAANSQNFANTAILVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYP QTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPM+
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMV 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           THCFSGSFLILAVVVL GAV SLVLAYRT+DFYKGDVYN+YREDMWIPQSDMEFYCIDDR
Sbjct: 511 THCFSGSFLILAVVVLLGAVVSLVLAYRTQDFYKGDVYNKYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLK 561
           KKG +NL RMDMPPKYNFLK
Sbjct: 571 KKGGNNLPRMDMPPKYNFLK 590

BLAST of CmoCh13G002220 vs. ExPASy TrEMBL
Match: A0A6J1HYK5 (uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251 PE=4 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 547/561 (97.50%), Postives = 555/561 (98.93%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRI KPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIVKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTV SMLFFLSIRPIKVRK P
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVVSMLFFLSIRPIKVRKHP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           +LFKLNKQTLNNNPTVPILMPEHEPSPKAPPTI+QIPEASTSCFSM FK+PQRGEDFTIL
Sbjct: 271 LLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIEQIPEASTSCFSMAFKRPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATL+ACGASIAAIDNLGQVGESLGYP QTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLSACGASIAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFIS+ALMTKYKLPRPLMFAFSH+LTC+GILFIAFPYKGSVYAASLIIGFGFGAQVPML
Sbjct: 391 SGFISDALMTKYKLPRPLMFAFSHILTCVGILFIAFPYKGSVYAASLIIGFGFGAQVPML 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIG+LYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGRLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           T CFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR
Sbjct: 511 TPCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLKS 562
           KKGDDNLARMDMPPKYNFLKS
Sbjct: 571 KKGDDNLARMDMPPKYNFLKS 591

BLAST of CmoCh13G002220 vs. ExPASy TrEMBL
Match: A0A6J1I3B9 (uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246 PE=4 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.6e-308
Identity = 542/560 (96.79%), Postives = 552/560 (98.57%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQF+YNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFNYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRI+KPPFWEMF+FICVAANSQNFANTAILVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTV SMLFFLSIRPIKVRK P
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVVSMLFFLSIRPIKVRKHP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           +LFKLNKQTLNNNPTVPILMPEHEPSPKAPPTI+QIPEASTSCFSM FK+PQRGEDFTIL
Sbjct: 271 LLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIEQIPEASTSCFSMAFKRPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATL+ACGASIAAIDNLGQVGESLGYP QTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLSACGASIAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPM+
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMV 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           T CFSGSFLILAVVVLFGAV SLVLAYRT+DFYKGDVYNRYREDMWIPQSDMEFYCIDDR
Sbjct: 511 TPCFSGSFLILAVVVLFGAVVSLVLAYRTQDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLK 561
           KKG +NL RMDMPPKYNFLK
Sbjct: 571 KKGGNNLPRMDMPPKYNFLK 590

BLAST of CmoCh13G002220 vs. ExPASy TrEMBL
Match: A0A5D3D1P8 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00170 PE=4 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 2.0e-242
Identity = 427/568 (75.18%), Postives = 489/568 (86.09%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTY+FGTYSK IK+QF+Y+QT+INTLGFAKDLGSN GVFAG LGE+APPWVL +V
Sbjct: 28  MIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKDLGSNLGVFAGFLGEIAPPWVLLVV 87

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           G+ LNF+SYFMIWLS+T RI+KP  W+MF +IC+AANSQNFANTA+LVTSVRNFPDRRGI
Sbjct: 88  GAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAANSQNFANTAVLVTSVRNFPDRRGI 147

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVG GGAILTQF+LALYGH+NP+NLVLLLSW PT+ S+ FFLSIR I +R+ P
Sbjct: 148 ILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLLSWFPTLISLAFFLSIRTINIRRHP 207

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           +ELRVLYHLLYVSIILALFLLFLTITQKQ  F+   Y SGA VI+GLL IPLLIA+REE 
Sbjct: 208 EELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAGYTSGAAVIVGLLSIPLLIAVREEL 267

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPP-------TIQQIPEA-STSCFSMTFKKPQ 300
           MLFKL  QT +NNP+ P+ +PE + S  + P        I++IPE  S +C S  F KP+
Sbjct: 268 MLFKLKGQT-DNNPSPPVFIPEMKSSSNSTPKNNESLTPIEEIPETNSPTCLSNVFNKPE 327

Query: 301 RGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSI 360
           RGEDFTILQALFSKDM L+ + TL  CG+SIAAIDN+GQ+GESLGY  ++ISIFVSWVSI
Sbjct: 328 RGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNIGQIGESLGYTSKSISIFVSWVSI 387

Query: 361 FNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFG 420
           FNFFGRV SGFISE LMTKYKLPRPLMFA +H  TCIG+LF+AFPY GS+Y ASLIIGFG
Sbjct: 388 FNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCIGMLFVAFPYPGSIYGASLIIGFG 447

Query: 421 FGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKN 480
           FGAQVPMLFAI+SELFGLKYY+TIFNC QLAVPIGSYILNVDVIGK YD EA K G  ++
Sbjct: 448 FGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSYILNVDVIGKFYDHEATKGGSTRD 507

Query: 481 GKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDM 540
           GKGLTC GTHCFSGSFL+L+VVVL G VASLVLA+RTRDFYKGDVY +YREDMWIPQSDM
Sbjct: 508 GKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRTRDFYKGDVYKKYREDMWIPQSDM 567

Query: 541 EFYCIDDRKK-GDDNLARMDMPPKYNFL 560
           EFYC+D++KK  ++NL R+ MPPKY+FL
Sbjct: 568 EFYCLDNKKKVVNNNLPRVVMPPKYSFL 594

BLAST of CmoCh13G002220 vs. NCBI nr
Match: XP_022964777.1 (uncharacterized protein LOC111464777 [Cucurbita moschata])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR
Sbjct: 511 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLKS 562
           KKGDDNLARMDMPPKYNFLKS
Sbjct: 571 KKGDDNLARMDMPPKYNFLKS 591

BLAST of CmoCh13G002220 vs. NCBI nr
Match: KAG6583549.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 558/561 (99.47%), Postives = 560/561 (99.82%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTA+LVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAVLVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQ+IPEASTSCFSMTFKKPQRGEDFTIL
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQKIPEASTSCFSMTFKKPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYP QTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR
Sbjct: 511 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLKS 562
           KKGDDNLARMDMPPKYNFLKS
Sbjct: 571 KKGDDNLARMDMPPKYNFLKS 591

BLAST of CmoCh13G002220 vs. NCBI nr
Match: KAG6583546.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 550/560 (98.21%), Postives = 556/560 (99.29%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRI+KPPFWEMF+FICVAANSQNFANTAILVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYP QTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPM+
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMV 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           THCFSGSFLILAVVVLFGAV SLVLAYRT+DFYKGDVYN+YREDMWIPQSDMEFYCIDDR
Sbjct: 511 THCFSGSFLILAVVVLFGAVVSLVLAYRTQDFYKGDVYNKYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLK 561
           KKG +NL RMDMPPKYNFLK
Sbjct: 571 KKGGNNLPRMDMPPKYNFLK 590

BLAST of CmoCh13G002220 vs. NCBI nr
Match: KAG6583531.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 548/560 (97.86%), Postives = 556/560 (99.29%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRI+KPPFWEMF+FICVAANSQNFANTA+LVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAVLVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQ+IPEASTSCFSMTFKKPQRGEDFTIL
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQKIPEASTSCFSMTFKKPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYP QTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPM+
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMV 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           THCFSGSFLILAVVVLFGAV SLVLAYRT+DFYKGDVYN+YREDMWIPQSDMEFYCIDDR
Sbjct: 511 THCFSGSFLILAVVVLFGAVVSLVLAYRTQDFYKGDVYNKYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLK 561
           KKG +NL RMDMPPKYNFLK
Sbjct: 571 KKGGNNLPRMDMPPKYNFLK 590

BLAST of CmoCh13G002220 vs. NCBI nr
Match: XP_022964772.1 (uncharacterized protein LOC111464772 [Cucurbita moschata])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 549/560 (98.04%), Postives = 555/560 (99.11%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV
Sbjct: 31  MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 90

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSALNFWSYFMIWLSITGRI+KPPFWEMF+FICVAANSQNFANTAILVTSVRNFPDRRGI
Sbjct: 91  GSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAANSQNFANTAILVTSVRNFPDRRGI 150

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP
Sbjct: 151 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 210

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF
Sbjct: 211 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 270

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 300
           MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL
Sbjct: 271 MLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFTIL 330

Query: 301 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGRVF 360
           QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYP QTISIFVSWVSIFNFFGRVF
Sbjct: 331 QALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVF 390

Query: 361 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPML 420
           SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPM+
Sbjct: 391 SGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMV 450

Query: 421 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 480
           FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG
Sbjct: 451 FAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTG 510

Query: 481 THCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDMEFYCIDDR 540
           THCFSGSFLILAVVVL GAV SLVLAYRT+DFYKGDVYN+YREDMWIPQSDMEFYCIDDR
Sbjct: 511 THCFSGSFLILAVVVLLGAVVSLVLAYRTQDFYKGDVYNKYREDMWIPQSDMEFYCIDDR 570

Query: 541 KKGDDNLARMDMPPKYNFLK 561
           KKG +NL RMDMPPKYNFLK
Sbjct: 571 KKGGNNLPRMDMPPKYNFLK 590

BLAST of CmoCh13G002220 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 565.8 bits (1457), Expect = 3.7e-161
Identity = 299/550 (54.36%), Postives = 389/550 (70.73%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           M  AG+TY+FGTYSK IKS   Y+QT +N LGF KDLG+N GV +GL+ EV P W +  +
Sbjct: 28  MACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLIAEVTPTWFVLTI 87

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           GSA+NF  YFMIWL++TG+++KP  W+M ++IC+ ANSQNFANT  LVT V+NFP+ RG+
Sbjct: 88  GSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGV 147

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           +LGLLKG+VG  GAI TQ Y A+YGHD+  +L+LL++WLP   S++F   IR  KV +  
Sbjct: 148 MLGLLKGYVGLSGAIFTQLYFAIYGHDS-KSLILLIAWLPAAVSLVFVYLIREKKVVRQR 207

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           +EL V Y  LY+SI LALFL+ + I +KQ  F++ AY + AT+   LL +PL +++++E 
Sbjct: 208 NELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLTVSVKQEL 267

Query: 241 MLFKLNKQTLNNNPTVPILMPEHE---PSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDF 300
            ++ + K  +     V +  P+ E      KA     +  E + SCFS  F  P RGED+
Sbjct: 268 EVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKE-TKSCFSTVFSPPPRGEDY 327

Query: 301 TILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFG 360
           TILQAL S DM ++F+AT    G+S+ A+DNLGQ+GESLGYP  T+S FVS VSI+N+FG
Sbjct: 328 TILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFVSLVSIWNYFG 387

Query: 361 RVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQV 420
           RVFSGF+SE L+ KYKLPRPLM     LL+C G L IAFP  GSVY AS+++GF FGAQ+
Sbjct: 388 RVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASILMGFSFGAQL 447

Query: 421 PMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAK---LGGV--KN 480
           P+LFAIISELFGLKYYST+FNCGQLA P+GSYILNV V G LYD EA K     G+  K+
Sbjct: 448 PLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQLTARGLTRKD 507

Query: 481 GKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYREDMWIPQSDM 540
            K LTC G+ C+   FLILA V  FGA+ SL LA RTR+FYKGD+Y ++RE    P+S+ 
Sbjct: 508 VKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFRES---PESES 567

Query: 541 EFYCIDDRKK 543
           E   + D +K
Sbjct: 568 EL--VPDSRK 570

BLAST of CmoCh13G002220 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 521.9 bits (1343), Expect = 6.2e-148
Identity = 277/548 (50.55%), Postives = 373/548 (68.07%), Query Frame = 0

Query: 1   MIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLV 60
           M  AG+TY+FG YS  IK    Y+QT +N L F KDLG+N GV AGLL EV PPW + L+
Sbjct: 32  MSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLI 91

Query: 61  GSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGI 120
           G+ LNF+ YFMIWL++T RISKP  W M ++ICV ANSQ+FANT  LVT V+NFP+ RG+
Sbjct: 92  GAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGV 151

Query: 121 ILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPIKVRKDP 180
           +LG+LKG+VG  GAI+TQ Y A YG D    L+L++ WLP + S  F  +IR +KV++  
Sbjct: 152 VLGILKGYVGLSGAIITQLYRAFYGEDT-KELILMIGWLPAIVSFAFLRTIRIMKVKRQT 211

Query: 181 DELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIREEF 240
           +EL+V Y+ LY+S+ LA FL+ + I  K + FTQ  +   A V+I LLL+P+++ I EE 
Sbjct: 212 NELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEK 271

Query: 241 MLFKLNKQTLNNNPTVPILMPEHEP-----------SPKAPPTIQQIPEASTSCFSMTFK 300
            L+K  KQ   N+P  PI +   +P             ++   ++++   + SC++  F 
Sbjct: 272 KLWK-EKQVALNDP-APINVVTEKPKLDSSEFKDDDGEESKEVVEKV--KTPSCWTTVFN 331

Query: 301 KPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSW 360
            P+RG+D+TILQALFS DM ++F+AT+   G ++ AIDNLGQ+G SLGYP +++S FVS 
Sbjct: 332 PPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSL 391

Query: 361 VSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLII 420
           VSI+N++GRV SG +SE  + KYK PRPLM     LL+C G L IAF   G +Y AS+II
Sbjct: 392 VSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVII 451

Query: 421 GFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAK--- 480
           GF FGAQ P+LFAIISE+FGLKYYST++N G +A PIGSY+LNV V G LYD+EA K   
Sbjct: 452 GFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYK 511

Query: 481 -LGGVK-NGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNRYRED 533
            LG  +  G+ L C GT CF  SF+I+A V LFG + S+VL  RT+ FYK D+Y ++RE 
Sbjct: 512 ALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREK 571

BLAST of CmoCh13G002220 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 8.0e-79
Identity = 180/516 (34.88%), Postives = 286/516 (55.43%), Query Frame = 0

Query: 4   AGSTYIF-GTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLVGS 63
           AG  Y+F G+ S AIK+   YNQ QI  LG AK+LG   G  +G L EV+P WV+ LVG+
Sbjct: 30  AGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGA 89

Query: 64  ALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGIIL 123
             N + Y ++WL +TG++   P W +FV I V  N + + NTA LV+ + NFP+ RG ++
Sbjct: 90  TQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVV 149

Query: 124 GLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPI----KVRK 183
           G+LKGF G  GAILTQ YL ++   + ++++L+++  P V  +     +RP+    +   
Sbjct: 150 GILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNL 209

Query: 184 DPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIRE 243
             D+LR L  +    ++LA++LL L + Q     TQ    +   +++  +++P+L+    
Sbjct: 210 RSDDLRFL-AIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSS 269

Query: 244 EFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFT 303
            F+          +   V  + PE   S       + + E S        + P  GEDFT
Sbjct: 270 VFI----------SGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEKK-RAPCIGEDFT 329

Query: 304 ILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGR 363
           +LQAL   D  L+F++ +   G+ I  IDNLGQ+  SLG  Y    IFVS +SI NF GR
Sbjct: 330 LLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLG--YSNTKIFVSLISISNFLGR 389

Query: 364 VFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVP 423
           V  G+ SE ++ K  LPR L  +    +  +G+++ A  + G +Y  +++IG G+GA   
Sbjct: 390 VAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWA 449

Query: 424 MLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGG-VKNGKGLT 483
           +  A +S++FGLK + +++N    A+PIGS++ +  +   +YD  A K  G     + L 
Sbjct: 450 IAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGPTTETESLV 509

Query: 484 CTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFY 514
           CTG+ C+S +  +++++ L   V SL + YRTR FY
Sbjct: 510 CTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of CmoCh13G002220 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 8.0e-79
Identity = 180/516 (34.88%), Postives = 286/516 (55.43%), Query Frame = 0

Query: 4   AGSTYIF-GTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLLGEVAPPWVLFLVGS 63
           AG  Y+F G+ S AIK+   YNQ QI  LG AK+LG   G  +G L EV+P WV+ LVG+
Sbjct: 30  AGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGA 89

Query: 64  ALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAILVTSVRNFPDRRGIIL 123
             N + Y ++WL +TG++   P W +FV I V  N + + NTA LV+ + NFP+ RG ++
Sbjct: 90  TQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVV 149

Query: 124 GLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLFFLSIRPI----KVRK 183
           G+LKGF G  GAILTQ YL ++   + ++++L+++  P V  +     +RP+    +   
Sbjct: 150 GILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNL 209

Query: 184 DPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGLLLIPLLIAIRE 243
             D+LR L  +    ++LA++LL L + Q     TQ    +   +++  +++P+L+    
Sbjct: 210 RSDDLRFL-AIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSS 269

Query: 244 EFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMTFKKPQRGEDFT 303
            F+          +   V  + PE   S       + + E S        + P  GEDFT
Sbjct: 270 VFI----------SGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEKK-RAPCIGEDFT 329

Query: 304 ILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFVSWVSIFNFFGR 363
           +LQAL   D  L+F++ +   G+ I  IDNLGQ+  SLG  Y    IFVS +SI NF GR
Sbjct: 330 LLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLG--YSNTKIFVSLISISNFLGR 389

Query: 364 VFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVP 423
           V  G+ SE ++ K  LPR L  +    +  +G+++ A  + G +Y  +++IG G+GA   
Sbjct: 390 VAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWA 449

Query: 424 MLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKLGG-VKNGKGLT 483
           +  A +S++FGLK + +++N    A+PIGS++ +  +   +YD  A K  G     + L 
Sbjct: 450 IAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGPTTETESLV 509

Query: 484 CTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFY 514
           CTG+ C+S +  +++++ L   V SL + YRTR FY
Sbjct: 510 CTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of CmoCh13G002220 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-77
Identity = 196/527 (37.19%), Postives = 292/527 (55.41%), Query Frame = 0

Query: 4   AGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSNFGVFAGLL--------------- 63
           +G++Y FG YS  +KS   Y+Q+ ++T+   KD+G+N GVF+GLL               
Sbjct: 21  SGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLYTYATSNRLRGRGGG 80

Query: 64  -GEVAPPWVLFLVGSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQNFANTAIL 123
            G    PWV+  VG+   F  YF+IW S+TG I KPP   M +F+ +AA SQ F NTA +
Sbjct: 81  IGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMFLAAQSQTFFNTANV 140

Query: 124 VTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLPTVASMLF 183
           V++V NF D  G  +G++KGF+G  GAIL Q Y  L   D P + +LLL+  PTV S+L 
Sbjct: 141 VSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD-PASFILLLAVTPTVLSLLV 200

Query: 184 FLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSGATVIIGL 243
              +R I      D+ + L  L  VS+I+A +L+ + I +     +  A +     ++ +
Sbjct: 201 MPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLSSWANIVTLVCLLVM 260

Query: 244 LLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEASTSCFSMT 303
           L +PLLIA R          Q      TVP        SPKA  +  Q  E  +      
Sbjct: 261 LALPLLIARR---------AQRDGMEKTVPHDYSPLISSPKATTSGNQSSEGDSKV---- 320

Query: 304 FKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPYQTISIFV 363
             +    E+  +LQA+      L+F+A +   G+ ++ I+N+ Q+GESL Y    I+  V
Sbjct: 321 --EAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSLV 380

Query: 364 SWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKGSVYAASL 423
           S  SI+NF GR  +G+ S+AL+ K   PRPL+ A +     IG L IA  ++G++Y  S+
Sbjct: 381 SLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGSV 440

Query: 424 IIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLYDIEAAKL 483
           I+G  +G+Q  ++  I SELFG+++  TIFN   +A PIGSYI +V +IG +YD  A   
Sbjct: 441 IVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTA--- 500

Query: 484 GGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYK 515
               +G+G TC G+HCF  SF+I+A V  FG + ++VL +RT+  Y+
Sbjct: 501 ----SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYR 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E16.9e-2726.25Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HIM60.0e+00100.00uncharacterized protein LOC111464777 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1HIM20.0e+0098.04uncharacterized protein LOC111464772 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1HYK50.0e+0097.50uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251... [more]
A0A6J1I3B91.6e-30896.79uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246... [more]
A0A5D3D1P82.0e-24275.18Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
XP_022964777.10.0e+00100.00uncharacterized protein LOC111464777 [Cucurbita moschata][more]
KAG6583549.10.0e+0099.47Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG6583546.10.0e+0098.21Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG6583531.10.0e+0097.86Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022964772.10.0e+0098.04uncharacterized protein LOC111464772 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G28120.13.7e-16154.36Major facilitator superfamily protein [more]
AT2G39210.16.2e-14850.55Major facilitator superfamily protein [more]
AT5G50630.18.0e-7934.88Major facilitator superfamily protein [more]
AT5G50520.18.0e-7934.88Major facilitator superfamily protein [more]
AT1G74780.11.5e-7737.19Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 4..236
e-value: 5.4E-75
score: 252.1
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 307..513
e-value: 5.1E-9
score: 37.8
coord: 1..179
e-value: 8.3E-6
score: 27.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 3..511
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 1..524
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 1..524
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 1..499
e-value: 8.12938E-96
score: 295.315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G002220.1CmoCh13G002220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0022857 transmembrane transporter activity
molecular_function GO:0008270 zinc ion binding