CmoCh13G002140 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G002140
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMajor facilitator superfamily protein
LocationCmo_Chr13: 1677660 .. 1679291 (-)
RNA-Seq ExpressionCmoCh13G002140
SyntenyCmoCh13G002140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCGGCTGCGGTTCACCTTACTTGTTCGGAACATACTCCAAACTCTTAAAAACAAAATTCGACTACAATCAAACCCAACTTAATACCTTGGGATTTGCTAAAGATCTCGGCTCCAACCTCGGAGTTTTTGCCGGGCTTTTCGCCGAGGTGGCTCCTCCGTGGATGCTTTTCCTTGTGGGGATATCCTCCAATTTCTTCAGCTACTTTATGATTTGGCTCTCCGTTTCTAGCTACGTCCCAAAACCCCATTTATGGCTAATGTTTGTTTACATCTATATTACTTCTAATTCACAGAACTTCCCCAACACCGCCGTTATGGTCACTAGCGTTGGAAATTTTCCTGATCAACGCGGTATCATTTTGGGCCTTCTCAAGGGTTTCGTTGGCCTCGGTGGTGCCATCCTAACTCAGATTAATTTAGCAATCCACGGCAACCAAAATTCTATGGATCTTCTCCTCCTTCTTTCATGGCTACCCTCCATTGTATGTTTCTTGTGTTTTCTTCCAATTCGGACAATCAAAGCTCGAAAGCATCCACAGGAGCTCAAAGTGTTCTACCACTTGCTTTACGTGTCCATAGCGATAGCGGTGTTCCTCTTGTTTCTCACCATAACCCAGAGAAACATTGCTTTCTCTCAAGCTGGCTATGCCGGTGGCGTCGCGGTCATCGGCGTCTTAATCTGTCTGCCCCTTCTAATAGCTATTAAAGAAGAACTTTTTCTCTTCAAACTCGGTAAACAAACCATGAATCCTTCTGTTGTTGTTTCGATTCCCCGTGAACAACTTAAGGAAATTTCAAAAATTTCTTTGCCGAGTGTCTTAAATGTATGTAAGGAGCCGCAAAGAGGAGAAGATTTCAGCATCTTGCAAGCTCTTCTAAGCAAAGACATGGCTCTTATTTTCATAGCAACGGTGTCTGCATGTGGGTCGTCCGTTGCAGCCATTGACAATCTTGGGCAGATCGCCGAGTCACTTAAATATCCAAATCATTCAATAAGTATTTTGGTTTCATGGATTTCCATATTCAATTTCTTTGGTCGGGTCTTCTCCGGTTTCATCTCTGAAACCCTTATGACCAAATACAAATTACCCCGCCCTTTCATGTTCGGTCTTACCCAAGTTTTTACCTGCACCGGCTTGCTCTCCATCGCATTCCCTTATATAAATTCAGTCTATGTAGCTTCGTTGATCATTGGGTTTGGCCTTGGAGCCCAAACTCCATTGATATTCGCCATAATCTCTGATCTCTTCGGCCTCAAACATTACTCGACACTGCTCAACTGTGGGCAACTGGCTGTCCCACTTGGATCTTATATAATGAACGTGGAAGTAATAGGGAGATTTTACGATGCAGAAGCCACTAAAATTGGAAATGTGAAGAATGGGAAGGGCCTAACTTGCACTGGAACTCATTGCTTTAGTGAATCGTTCATGATTTTGGCTGCAGTGACGTTGTTTGGAGCGATGGCTTCATTTGTACTATCTTATAGAACTCGAGACTTCTACAAAGGTGATGTATATAAGAAATACAGAGATGACATGGAGGTTAAGCATTCGAACGTGGAATTATATTCTTCCGATGATAAGAACAATGAACACAAAATGAAAACTGATGATCAAAGTAAATAA

mRNA sequence

ATGATCGGCTGCGGTTCACCTTACTTGTTCGGAACATACTCCAAACTCTTAAAAACAAAATTCGACTACAATCAAACCCAACTTAATACCTTGGGATTTGCTAAAGATCTCGGCTCCAACCTCGGAGTTTTTGCCGGGCTTTTCGCCGAGGTGGCTCCTCCGTGGATGCTTTTCCTTGTGGGGATATCCTCCAATTTCTTCAGCTACTTTATGATTTGGCTCTCCGTTTCTAGCTACGTCCCAAAACCCCATTTATGGCTAATGTTTGTTTACATCTATATTACTTCTAATTCACAGAACTTCCCCAACACCGCCGTTATGGTCACTAGCGTTGGAAATTTTCCTGATCAACGCGGTATCATTTTGGGCCTTCTCAAGGGTTTCGTTGGCCTCGGTGGTGCCATCCTAACTCAGATTAATTTAGCAATCCACGGCAACCAAAATTCTATGGATCTTCTCCTCCTTCTTTCATGGCTACCCTCCATTGTATGTTTCTTGTGTTTTCTTCCAATTCGGACAATCAAAGCTCGAAAGCATCCACAGGAGCTCAAAGTGTTCTACCACTTGCTTTACGTGTCCATAGCGATAGCGGTGTTCCTCTTGTTTCTCACCATAACCCAGAGAAACATTGCTTTCTCTCAAGCTGGCTATGCCGGTGGCGTCGCGGTCATCGGCGTCTTAATCTGTCTGCCCCTTCTAATAGCTATTAAAGAAGAACTTTTTCTCTTCAAACTCGGTAAACAAACCATGAATCCTTCTGTTGTTGTTTCGATTCCCCGTGAACAACTTAAGGAAATTTCAAAAATTTCTTTGCCGAGTGTCTTAAATGTATGTAAGGAGCCGCAAAGAGGAGAAGATTTCAGCATCTTGCAAGCTCTTCTAAGCAAAGACATGGCTCTTATTTTCATAGCAACGGTGTCTGCATGTGGGTCGTCCGTTGCAGCCATTGACAATCTTGGGCAGATCGCCGAGTCACTTAAATATCCAAATCATTCAATAAGTATTTTGGTTTCATGGATTTCCATATTCAATTTCTTTGGTCGGGTCTTCTCCGGTTTCATCTCTGAAACCCTTATGACCAAATACAAATTACCCCGCCCTTTCATGTTCGGTCTTACCCAAGTTTTTACCTGCACCGGCTTGCTCTCCATCGCATTCCCTTATATAAATTCAGTCTATGTAGCTTCGTTGATCATTGGGTTTGGCCTTGGAGCCCAAACTCCATTGATATTCGCCATAATCTCTGATCTCTTCGGCCTCAAACATTACTCGACACTGCTCAACTGTGGGCAACTGGCTGTCCCACTTGGATCTTATATAATGAACGTGGAAGTAATAGGGAGATTTTACGATGCAGAAGCCACTAAAATTGGAAATGTGAAGAATGGGAAGGGCCTAACTTGCACTGGAACTCATTGCTTTAGTGAATCGTTCATGATTTTGGCTGCAGTGACGTTGTTTGGAGCGATGGCTTCATTTGTACTATCTTATAGAACTCGAGACTTCTACAAAGGTGATGTATATAAGAAATACAGAGATGACATGGAGGTTAAGCATTCGAACGTGGAATTATATTCTTCCGATGATAAGAACAATGAACACAAAATGAAAACTGATGATCAAAGTAAATAA

Coding sequence (CDS)

ATGATCGGCTGCGGTTCACCTTACTTGTTCGGAACATACTCCAAACTCTTAAAAACAAAATTCGACTACAATCAAACCCAACTTAATACCTTGGGATTTGCTAAAGATCTCGGCTCCAACCTCGGAGTTTTTGCCGGGCTTTTCGCCGAGGTGGCTCCTCCGTGGATGCTTTTCCTTGTGGGGATATCCTCCAATTTCTTCAGCTACTTTATGATTTGGCTCTCCGTTTCTAGCTACGTCCCAAAACCCCATTTATGGCTAATGTTTGTTTACATCTATATTACTTCTAATTCACAGAACTTCCCCAACACCGCCGTTATGGTCACTAGCGTTGGAAATTTTCCTGATCAACGCGGTATCATTTTGGGCCTTCTCAAGGGTTTCGTTGGCCTCGGTGGTGCCATCCTAACTCAGATTAATTTAGCAATCCACGGCAACCAAAATTCTATGGATCTTCTCCTCCTTCTTTCATGGCTACCCTCCATTGTATGTTTCTTGTGTTTTCTTCCAATTCGGACAATCAAAGCTCGAAAGCATCCACAGGAGCTCAAAGTGTTCTACCACTTGCTTTACGTGTCCATAGCGATAGCGGTGTTCCTCTTGTTTCTCACCATAACCCAGAGAAACATTGCTTTCTCTCAAGCTGGCTATGCCGGTGGCGTCGCGGTCATCGGCGTCTTAATCTGTCTGCCCCTTCTAATAGCTATTAAAGAAGAACTTTTTCTCTTCAAACTCGGTAAACAAACCATGAATCCTTCTGTTGTTGTTTCGATTCCCCGTGAACAACTTAAGGAAATTTCAAAAATTTCTTTGCCGAGTGTCTTAAATGTATGTAAGGAGCCGCAAAGAGGAGAAGATTTCAGCATCTTGCAAGCTCTTCTAAGCAAAGACATGGCTCTTATTTTCATAGCAACGGTGTCTGCATGTGGGTCGTCCGTTGCAGCCATTGACAATCTTGGGCAGATCGCCGAGTCACTTAAATATCCAAATCATTCAATAAGTATTTTGGTTTCATGGATTTCCATATTCAATTTCTTTGGTCGGGTCTTCTCCGGTTTCATCTCTGAAACCCTTATGACCAAATACAAATTACCCCGCCCTTTCATGTTCGGTCTTACCCAAGTTTTTACCTGCACCGGCTTGCTCTCCATCGCATTCCCTTATATAAATTCAGTCTATGTAGCTTCGTTGATCATTGGGTTTGGCCTTGGAGCCCAAACTCCATTGATATTCGCCATAATCTCTGATCTCTTCGGCCTCAAACATTACTCGACACTGCTCAACTGTGGGCAACTGGCTGTCCCACTTGGATCTTATATAATGAACGTGGAAGTAATAGGGAGATTTTACGATGCAGAAGCCACTAAAATTGGAAATGTGAAGAATGGGAAGGGCCTAACTTGCACTGGAACTCATTGCTTTAGTGAATCGTTCATGATTTTGGCTGCAGTGACGTTGTTTGGAGCGATGGCTTCATTTGTACTATCTTATAGAACTCGAGACTTCTACAAAGGTGATGTATATAAGAAATACAGAGATGACATGGAGGTTAAGCATTCGAACGTGGAATTATATTCTTCCGATGATAAGAACAATGAACACAAAATGAAAACTGATGATCAAAGTAAATAA

Protein sequence

MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEELFLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDDQSK
Homology
BLAST of CmoCh13G002140 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 9.7e-26
Identity = 128/520 (24.62%), Postives = 234/520 (45.00%), Query Frame = 0

Query: 5   GSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLVGISS 64
           G+ + F  YS  LK+    +Q +LN L  A DLG   G  +G+     P  ++     + 
Sbjct: 58  GTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAAAM 117

Query: 65  NFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGIILGL 124
            F  Y + WL +++ +  P+  L+F+   +   S  + NTA  +  + +FP+ R + L L
Sbjct: 118 GFVGYGVQWLVITNIITLPYS-LVFLCCLLAGLSICWFNTACFILCIRHFPNNRALALSL 177

Query: 125 LKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRT---------IK 184
              F G+  A+ +    AI+ + +++  LLL S +P +V F    P+ T           
Sbjct: 178 TVSFNGISAALYSLAFNAINPSSSNL-YLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYD 237

Query: 185 ARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIA 244
           +R+H   +   + +L V   I  F L L+ +  + A  +  + G V ++   +C PLL+ 
Sbjct: 238 SRRHDSHV---FTILNVLAVITSFHLLLSSSSTSSA--RLNFIGAVVLLVFPLCAPLLVY 297

Query: 245 IKEELFLFKLGKQTMNPSVVVSIPREQLKEISKISLPSV-----LNVCKE---PQRGEDF 304
            ++        +     S  V +  ++LK   K S+ S      +   KE    + G++ 
Sbjct: 298 ARDYFLPVINARLNHESSGYVMLNIDELKN-QKTSVSSKTGYEHMGTAKEGNTVRLGDEH 357

Query: 305 SILQALLSKDMALIFIATVSACGSSVAAI--DNLGQIAESLKYPNHSISILVSWISIFNF 364
           S    +   +  L +IA    CG ++  +  +NLGQIA+SL     + + LV+  S F+F
Sbjct: 358 SFRLLISRLEFWLYYIAYF--CGGTIGLVYSNNLGQIAQSL---GQNSTTLVTIYSSFSF 417

Query: 365 FGRVFSGFISETLMTKYKLPRPFMFGLTQVFTCTG--LLSIAFPYINSVYVASLIIGFGL 424
           FGR+ S    + +  +++L R   F +  + T     LL+++     ++  A+ +IG   
Sbjct: 418 FGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSS 477

Query: 425 GAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNG 484
           G       +I SDLFG        N     +P+GS +    +    Y+A A+        
Sbjct: 478 GFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYEANASPDITPIVS 537

Query: 485 KGLTCTGTHCFSESFMILAAVTLFGAMASFVLSYRTRDFY 504
             + C G  C+ ++F+    +++ G ++S  L  RT+  Y
Sbjct: 538 DSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CmoCh13G002140 vs. ExPASy TrEMBL
Match: A0A6J1HP67 (uncharacterized protein LOC111464769 OS=Cucurbita moschata OX=3662 GN=LOC111464769 PE=4 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 3.0e-304
Identity = 541/542 (99.82%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI
Sbjct: 91  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL
Sbjct: 271 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD
Sbjct: 511 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 570

Query: 541 QS 543
           Q+
Sbjct: 571 QN 572

BLAST of CmoCh13G002140 vs. ExPASy TrEMBL
Match: A0A6J1I3Q9 (uncharacterized protein LOC111469397 OS=Cucurbita maxima OX=3661 GN=LOC111469397 PE=4 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 1.4e-290
Identity = 516/543 (95.03%), Postives = 530/543 (97.61%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVS+YVPKPHLWLMFVYIYITSNSQNFPNTAV+VTSVGNFPDQRGI
Sbjct: 91  GISSNFFSYFMIWLSVSNYVPKPHLWLMFVYIYITSNSQNFPNTAVVVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAI+GNQNS+DLLLLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIYGNQNSIDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVI VLICLPLLIAIKEEL
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIVVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
            LFKLGKQTMNPSV+VSIPRE L++ISKISLPSV NVCK PQRGEDFSILQALLSKDM L
Sbjct: 271 SLFKLGKQTMNPSVLVSIPREPLEQISKISLPSVSNVCKGPQRGEDFSILQALLSKDMTL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIAT+SACGSSVAAIDNLGQIAESLKYPNHS+SILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATISACGSSVAAIDNLGQIAESLKYPNHSMSILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQ+FTCTGLLSIAFPY+NSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFMFGLTQLFTCTGLLSIAFPYVNSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCF ESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFGESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LA +TLFGAMASFVL+YRTRDFYKGDVYKKYRDD+EV  SNVELYSSDDKNNEHK KTDD
Sbjct: 511 LATLTLFGAMASFVLAYRTRDFYKGDVYKKYRDDIEVAQSNVELYSSDDKNNEHKKKTDD 570

Query: 541 QSK 544
             K
Sbjct: 571 PIK 573

BLAST of CmoCh13G002140 vs. ExPASy TrEMBL
Match: A0A6J1I4Z4 (uncharacterized protein LOC111469253 OS=Cucurbita maxima OX=3661 GN=LOC111469253 PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.2e-290
Identity = 515/543 (94.84%), Postives = 531/543 (97.79%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVS+YVPKPHLWLMFVYIYITSNSQNFPNTAVMVT+V NFP+QRGI
Sbjct: 91  GISSNFFSYFMIWLSVSNYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTNVSNFPNQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAI+GNQNS+DLLLLLSWLP+IVCFLCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIYGNQNSIDLLLLLSWLPTIVCFLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           +ELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGG+ VI VLICLPLLIAIKEEL
Sbjct: 211 RELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGIVVIVVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVS+PREQL+EISK+SLPSV NVCKEPQRGEDF+ILQALLSKDMAL
Sbjct: 271 FLFKLGKQTMNPSVVVSLPREQLEEISKLSLPSVSNVCKEPQRGEDFNILQALLSKDMAL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQ+FTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFMFGLTQLFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFG MASFVL+YRTRDFYKGDVYKKYRDDME   SNVEL S DDKN+EHK K DD
Sbjct: 511 LAAVTLFGTMASFVLAYRTRDFYKGDVYKKYRDDMEATQSNVELNSFDDKNSEHKKKIDD 570

Query: 541 QSK 544
           +SK
Sbjct: 571 ESK 573

BLAST of CmoCh13G002140 vs. ExPASy TrEMBL
Match: A0A6J1HYK0 (uncharacterized protein LOC111469245 OS=Cucurbita maxima OX=3661 GN=LOC111469245 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.6e-289
Identity = 514/543 (94.66%), Postives = 529/543 (97.42%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVS+YVPKPHLWLMFVYIYITSNSQNFPNTAV+VTSVGNFPDQRGI
Sbjct: 91  GISSNFFSYFMIWLSVSNYVPKPHLWLMFVYIYITSNSQNFPNTAVVVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAI+G+QNS+DL LLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIYGSQNSIDLFLLLSWLPSIVCFLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVI VLICLPLLIAIKEEL
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIVVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
            LFKLGKQTMNPSV+VSIPRE L++ISKISLPSV NVCK PQRGEDFSILQALLSKDM L
Sbjct: 271 SLFKLGKQTMNPSVLVSIPREPLEQISKISLPSVSNVCKGPQRGEDFSILQALLSKDMTL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIAT+SACGSSVAAIDNLGQIAESLKYPNHS+SILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATISACGSSVAAIDNLGQIAESLKYPNHSMSILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQ+FTCTGLLSIAFPY+NSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFMFGLTQLFTCTGLLSIAFPYVNSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCF ESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFGESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LA +TLFGAMASFVL+YRTRDFYKGDVYKKYRDD+EV  SNVELYSSDDKNNEHK KTDD
Sbjct: 511 LATLTLFGAMASFVLAYRTRDFYKGDVYKKYRDDIEVAQSNVELYSSDDKNNEHKKKTDD 570

Query: 541 QSK 544
             K
Sbjct: 571 PIK 573

BLAST of CmoCh13G002140 vs. ExPASy TrEMBL
Match: A0A6J1HP71 (uncharacterized protein LOC111464779 OS=Cucurbita moschata OX=3662 GN=LOC111464779 PE=4 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.4e-287
Identity = 516/543 (95.03%), Postives = 525/543 (96.69%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVS+YV KP LWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI
Sbjct: 91  GISSNFFSYFMIWLSVSNYVSKPQLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAI+GNQNSMDLLLLLSWLPSIVC LCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIYGNQNSMDLLLLLSWLPSIVCSLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNI FSQAGYA GVAVI VLICLPLLIAIKEEL
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIVFSQAGYASGVAVIVVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVS+P EQL+EISK SLPSV NVCK+PQRGEDFSILQALLSKDMAL
Sbjct: 271 FLFKLGKQTMNPSVVVSLPCEQLEEISKNSLPSVSNVCKKPQRGEDFSILQALLSKDMAL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESLKYPNHSI ILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSIGILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQ+FT TGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFMFGLTQLFTSTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFGAMASFVL+YRTRDFYKGDVYKKY+DDME   SNVEL S DDKNNEHK  TDD
Sbjct: 511 LAAVTLFGAMASFVLAYRTRDFYKGDVYKKYKDDMEATQSNVELNSFDDKNNEHKKITDD 570

Query: 541 QSK 544
           +SK
Sbjct: 571 RSK 573

BLAST of CmoCh13G002140 vs. NCBI nr
Match: XP_022964769.1 (uncharacterized protein LOC111464769 [Cucurbita moschata])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.2e-304
Identity = 541/542 (99.82%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI
Sbjct: 91  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL
Sbjct: 271 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD
Sbjct: 511 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 570

Query: 541 QS 543
           Q+
Sbjct: 571 QN 572

BLAST of CmoCh13G002140 vs. NCBI nr
Match: KAG6583539.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.5e-302
Identity = 539/543 (99.26%), Postives = 541/543 (99.63%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI
Sbjct: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           +ELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL
Sbjct: 181 RELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSV NVCKEPQRGEDFSILQALLSKDMAL
Sbjct: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVSNVCKEPQRGEDFSILQALLSKDMAL 300

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFGAMASFVL+YRTRDFYKGDVYKKYRDDMEVKHSN ELYSSDDKNNEHKMKTDD
Sbjct: 481 LAAVTLFGAMASFVLAYRTRDFYKGDVYKKYRDDMEVKHSNEELYSSDDKNNEHKMKTDD 540

Query: 541 QSK 544
           QSK
Sbjct: 541 QSK 543

BLAST of CmoCh13G002140 vs. NCBI nr
Match: KAG6583527.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1046.2 bits (2704), Expect = 9.8e-302
Identity = 538/543 (99.08%), Postives = 540/543 (99.45%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI
Sbjct: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL
Sbjct: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVSIPREQLKEISKISL SV NVCKEPQRGEDFSILQALLSKDMAL
Sbjct: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLRSVSNVCKEPQRGEDFSILQALLSKDMAL 300

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESL+YPNHSISILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 301 IFIATVSACGSSVAAIDNLGQIAESLEYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFGAMASFVL+YRTRDFYKGDVYKKYRDDMEVKHSN ELYSSDDKNNEHKMKTDD
Sbjct: 481 LAAVTLFGAMASFVLAYRTRDFYKGDVYKKYRDDMEVKHSNAELYSSDDKNNEHKMKTDD 540

Query: 541 QSK 544
           QSK
Sbjct: 541 QSK 543

BLAST of CmoCh13G002140 vs. NCBI nr
Match: XP_023520298.1 (uncharacterized protein LOC111783614 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.6e-299
Identity = 535/543 (98.53%), Postives = 538/543 (99.08%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI
Sbjct: 91  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAIHGNQNS+DLLLLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIHGNQNSIDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVI VLICLPLLIAIKEEL
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIIVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSV NVCKEPQRGEDFSILQALLSKDMAL
Sbjct: 271 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVSNVCKEPQRGEDFSILQALLSKDMAL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPF+FGLTQVFTC GLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFLFGLTQVFTCIGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFGAMAS VL+YRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKT D
Sbjct: 511 LAAVTLFGAMASLVLAYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTHD 570

Query: 541 QSK 544
           QSK
Sbjct: 571 QSK 573

BLAST of CmoCh13G002140 vs. NCBI nr
Match: XP_023521328.1 (uncharacterized protein LOC111785084 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1036.6 bits (2679), Expect = 7.8e-299
Identity = 534/543 (98.34%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV
Sbjct: 31  MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 90

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI
Sbjct: 91  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 150

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           ILGLLKGFVGLGGAILTQINLAIHGNQNS+DLLLLLSWLPSIVCFLCFLPIRTIKARKHP
Sbjct: 151 ILGLLKGFVGLGGAILTQINLAIHGNQNSIDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 210

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
           QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVI VLICLPLLIAIKEEL
Sbjct: 211 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIVVLICLPLLIAIKEEL 270

Query: 241 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQRGEDFSILQALLSKDMAL 300
           FLFKLGKQTMNPSVVVSIPREQLKEISK SLPSV NVCKEPQRGEDFSILQALLSKDMAL
Sbjct: 271 FLFKLGKQTMNPSVVVSIPREQLKEISKNSLPSVSNVCKEPQRGEDFSILQALLSKDMAL 330

Query: 301 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 360
           IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT
Sbjct: 331 IFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMT 390

Query: 361 KYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 420
           KYKLPRPF+FGLTQVFTC GLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL
Sbjct: 391 KYKLPRPFLFGLTQVFTCIGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGL 450

Query: 421 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 480
           KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI
Sbjct: 451 KHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNVKNGKGLTCTGTHCFSESFMI 510

Query: 481 LAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTDD 540
           LAAVTLFGAMAS VL+YRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKT D
Sbjct: 511 LAAVTLFGAMASLVLAYRTRDFYKGDVYKKYRDDMEVKHSNVELYSSDDKNNEHKMKTHD 570

Query: 541 QSK 544
           QSK
Sbjct: 571 QSK 573

BLAST of CmoCh13G002140 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 506.1 bits (1302), Expect = 3.4e-143
Identity = 273/533 (51.22%), Postives = 361/533 (67.73%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           M   G+ YLFGTYSK +K+   Y+QT LN LGF KDLG+N+GV +GL AEV P W +  +
Sbjct: 28  MACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLIAEVTPTWFVLTI 87

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           G + NF  YFMIWL+V+  V KP +W M +YI I +NSQNF NT  +VT V NFP+ RG+
Sbjct: 88  GSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGV 147

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           +LGLLKG+VGL GAI TQ+  AI+G+ +S  L+LL++WLP+ V  +    IR  K  +  
Sbjct: 148 MLGLLKGYVGLSGAIFTQLYFAIYGH-DSKSLILLIAWLPAAVSLVFVYLIREKKVVRQR 207

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
            EL VFY  LY+SI +A+FL+ + I ++ + FS+A YA    +   L+ +PL +++K+EL
Sbjct: 208 NELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLTVSVKQEL 267

Query: 241 FLFKLGKQTM-NPS-VVVSIPREQL----------KEISKISLPSVLNVCKEPQRGEDFS 300
            ++ + K  +  PS V V  P+++L              K +      V   P RGED++
Sbjct: 268 EVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTVFSPPPRGEDYT 327

Query: 301 ILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGR 360
           ILQALLS DM ++F+AT    GSS+ A+DNLGQI ESL YPNH++S  VS +SI+N+FGR
Sbjct: 328 ILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFVSLVSIWNYFGR 387

Query: 361 VFSGFISETLMTKYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTP 420
           VFSGF+SE L+ KYKLPRP M  L  + +C G L IAFP   SVY+AS+++GF  GAQ P
Sbjct: 388 VFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASILMGFSFGAQLP 447

Query: 421 LIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATKIGNV-----KNG 480
           L+FAIIS+LFGLK+YSTL NCGQLA PLGSYI+NV V G  YD EA K         K+ 
Sbjct: 448 LLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQLTARGLTRKDV 507

Query: 481 KGLTCTGTHCFSESFMILAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRDDME 517
           K LTC G+ C+   F+ILAAVT FGA+ S  L+ RTR+FYKGD+YKK+R+  E
Sbjct: 508 KDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFRESPE 559

BLAST of CmoCh13G002140 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 474.6 bits (1220), Expect = 1.1e-133
Identity = 254/535 (47.48%), Postives = 353/535 (65.98%), Query Frame = 0

Query: 1   MIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLV 60
           M   G+ Y+FG YS  +K    Y+QT LN L F KDLG+N+GV AGL  EV PPW + L+
Sbjct: 32  MSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLI 91

Query: 61  GISSNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGI 120
           G   NFF YFMIWL+V+  + KP +W M +YI + +NSQ+F NT  +VT V NFP+ RG+
Sbjct: 92  GAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGV 151

Query: 121 ILGLLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARKHP 180
           +LG+LKG+VGL GAI+TQ+  A +G +++ +L+L++ WLP+IV F     IR +K ++  
Sbjct: 152 VLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQT 211

Query: 181 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 240
            ELKVFY+ LY+S+ +A FL+ + I  +   F+Q+ + G  AV+ VL+ LP+++ I EE 
Sbjct: 212 NELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEK 271

Query: 241 FLFKLGKQTMNP----SVVVSIPR-----------EQLKE-ISKISLPSV-LNVCKEPQR 300
            L+K  +  +N     +VV   P+           E+ KE + K+  PS    V   P+R
Sbjct: 272 KLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPER 331

Query: 301 GEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIF 360
           G+D++ILQAL S DM ++F+AT+   G ++ AIDNLGQI  SL YP  S+S  VS +SI+
Sbjct: 332 GDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSIW 391

Query: 361 NFFGRVFSGFISETLMTKYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGL 420
           N++GRV SG +SE  + KYK PRP M  +  + +C G L IAF     +YVAS+IIGF  
Sbjct: 392 NYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCF 451

Query: 421 GAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATK----IGN 480
           GAQ PL+FAIIS++FGLK+YSTL N G +A P+GSY++NV V G  YD EA K    +G 
Sbjct: 452 GAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALGK 511

Query: 481 VK-NGKGLTCTGTHCFSESFMILAAVTLFGAMASFVLSYRTRDFYKGDVYKKYRD 514
            +  G+ L C GT CF  SF+I+AAVTLFG + S VL  RT+ FYK D+YKK+R+
Sbjct: 512 TRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFRE 565

BLAST of CmoCh13G002140 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 3.4e-74
Identity = 176/511 (34.44%), Postives = 275/511 (53.82%), Query Frame = 0

Query: 5   GSPYLF-GTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLVGIS 64
           G  YLF G+ S  +KT   YNQ Q+  LG AK+LG  +G  +G  +EV+P W++ LVG +
Sbjct: 31  GVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGAT 90

Query: 65  SNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGIILG 124
            N F Y ++WL V+  +P   LW++FV I++ +N + + NTA +V+ + NFP+ RG ++G
Sbjct: 91  QNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVVG 150

Query: 125 LLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFL--PI-RTIKARKHP 184
           +LKGF GL GAILTQ+ L  + + +S  +L++    P +V  L F+  P+ R+ +     
Sbjct: 151 ILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNLRS 210

Query: 185 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 244
            +L+ F  +    + +AV+LL L + Q     +Q       A++ + + +P+L+      
Sbjct: 211 DDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSS-- 270

Query: 245 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQR-------GEDFSILQAL 304
            +F  G      +V    P E    + +    +++     P         GEDF++LQAL
Sbjct: 271 -VFISGN-----NVTSVKPEEGTSNVDQHEARTLIERSDRPPEKKRAPCIGEDFTLLQAL 330

Query: 305 LSKDMALIFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGF 364
              D  LIF++ V   GS +  IDNLGQI  SL Y N    I VS ISI NF GRV  G+
Sbjct: 331 GQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSN--TKIFVSLISISNFLGRVAGGY 390

Query: 365 ISETLMTKYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAI 424
            SE ++ K  LPR     + Q     GL+  A  +   +YV +++IG G GA   +  A 
Sbjct: 391 FSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPAS 450

Query: 425 ISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATK-IGNVKNGKGLTCTGTH 484
           +SD+FGLK + +L N    A+P+GS++ +  +    YD  A K  G     + L CTG+ 
Sbjct: 451 VSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGPTTETESLVCTGSV 510

Query: 485 CFSESFMILAAVTLFGAMASFVLSYRTRDFY 504
           C+S +  +++ + L   + S  + YRTR FY
Sbjct: 511 CYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of CmoCh13G002140 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 3.4e-74
Identity = 176/511 (34.44%), Postives = 275/511 (53.82%), Query Frame = 0

Query: 5   GSPYLF-GTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLVGIS 64
           G  YLF G+ S  +KT   YNQ Q+  LG AK+LG  +G  +G  +EV+P W++ LVG +
Sbjct: 31  GVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGAT 90

Query: 65  SNFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGIILG 124
            N F Y ++WL V+  +P   LW++FV I++ +N + + NTA +V+ + NFP+ RG ++G
Sbjct: 91  QNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVVG 150

Query: 125 LLKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFL--PI-RTIKARKHP 184
           +LKGF GL GAILTQ+ L  + + +S  +L++    P +V  L F+  P+ R+ +     
Sbjct: 151 ILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNLRS 210

Query: 185 QELKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEEL 244
            +L+ F  +    + +AV+LL L + Q     +Q       A++ + + +P+L+      
Sbjct: 211 DDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSS-- 270

Query: 245 FLFKLGKQTMNPSVVVSIPREQLKEISKISLPSVLNVCKEPQR-------GEDFSILQAL 304
            +F  G      +V    P E    + +    +++     P         GEDF++LQAL
Sbjct: 271 -VFISGN-----NVTSVKPEEGTSNVDQHEARTLIERSDRPPEKKRAPCIGEDFTLLQAL 330

Query: 305 LSKDMALIFIATVSACGSSVAAIDNLGQIAESLKYPNHSISILVSWISIFNFFGRVFSGF 364
              D  LIF++ V   GS +  IDNLGQI  SL Y N    I VS ISI NF GRV  G+
Sbjct: 331 GQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSN--TKIFVSLISISNFLGRVAGGY 390

Query: 365 ISETLMTKYKLPRPFMFGLTQVFTCTGLLSIAFPYINSVYVASLIIGFGLGAQTPLIFAI 424
            SE ++ K  LPR     + Q     GL+  A  +   +YV +++IG G GA   +  A 
Sbjct: 391 FSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPAS 450

Query: 425 ISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGRFYDAEATK-IGNVKNGKGLTCTGTH 484
           +SD+FGLK + +L N    A+P+GS++ +  +    YD  A K  G     + L CTG+ 
Sbjct: 451 VSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGPTTETESLVCTGSV 510

Query: 485 CFSESFMILAAVTLFGAMASFVLSYRTRDFY 504
           C+S +  +++ + L   + S  + YRTR FY
Sbjct: 511 CYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of CmoCh13G002140 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.7e-70
Identity = 178/543 (32.78%), Postives = 278/543 (51.20%), Query Frame = 0

Query: 5   GSPYLFGTYSKLLKTKFDYNQTQLNTLGFAKDLGSNLGVFAGLFAEVAPPWMLFLVGISS 64
           G  YLFG+ S ++K+  +YNQ +L+ LG AKDLG ++G  AG  +E+ P W   LVG   
Sbjct: 31  GIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLSEILPLWAALLVGAVQ 90

Query: 65  NFFSYFMIWLSVSSYVPKPHLWLMFVYIYITSNSQNFPNTAVMVTSVGNFPDQRGIILGL 124
           N   Y  +WL V+   P   LW M V I++ +N + + NT  +V+ V NFP  RG ++G+
Sbjct: 91  NLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVSGVQNFPKSRGPVVGI 150

Query: 125 LKGFVGLGGAILTQINLAIHGNQNSMDLLLLLSWLPSIVCFLCFLPIRTIKARK--HPQE 184
           LKGF GLGGAI++QI   IH + N   L+L+++  P++V       IR +   K   P +
Sbjct: 151 LKGFAGLGGAIISQIYTMIH-SSNPASLILMVAVTPAVVVVCLMFFIRPVGGHKQIRPTD 210

Query: 185 LKVFYHLLYVSIAIAVFLLFLTITQRNIAFSQAGYAGGVAVIGVLICLPLLIAIKEELFL 244
              F  +  V + +A +L+ + + Q  +  S         V+ V++ +P+L+ I    F 
Sbjct: 211 GASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVILVVPILVPIMTSFFT 270

Query: 245 -------------------FKLGKQTMN----------PSVVVSIP-REQLKEISKISLP 304
                               + G QT +          P  V  +P  E+ K I+ +   
Sbjct: 271 ETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLPASERHKRIAHLQAQ 330

Query: 305 SV---------LNVCKEPQRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIA 364
            +         +N  + P RGEDF++ QAL+  D  LIF + +   GS +  IDNLGQ++
Sbjct: 331 LMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMS 390

Query: 365 ESLKYPNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQVFTCTGLLS 424
           +SL Y N    +LVS ISI+NF GR+  G+ SE ++  Y  PRP    + Q+    G + 
Sbjct: 391 QSLGYDN--THVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVAMAVAQLIMSVGHIF 450

Query: 425 IAFPYINSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNV 484
            A+ +  ++Y+ +L+IG G GA   ++ A  S+LFGLK +  L N   LA P GS + + 
Sbjct: 451 FAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSG 510

Query: 485 EVIGRFYDAEATK--IGNVKN-GKGLTCTGTHCFSESFMILAAVTLFGAMASFVLSYRTR 504
            +    YD EA +   G+V +    L C G+ CF  + +I++   +   M S +L  RT+
Sbjct: 511 MIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTK 570

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E19.7e-2624.62Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HP673.0e-30499.82uncharacterized protein LOC111464769 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1I3Q91.4e-29095.03uncharacterized protein LOC111469397 OS=Cucurbita maxima OX=3661 GN=LOC111469397... [more]
A0A6J1I4Z43.2e-29094.84uncharacterized protein LOC111469253 OS=Cucurbita maxima OX=3661 GN=LOC111469253... [more]
A0A6J1HYK01.6e-28994.66uncharacterized protein LOC111469245 OS=Cucurbita maxima OX=3661 GN=LOC111469245... [more]
A0A6J1HP717.4e-28795.03uncharacterized protein LOC111464779 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
Match NameE-valueIdentityDescription
XP_022964769.16.2e-30499.82uncharacterized protein LOC111464769 [Cucurbita moschata][more]
KAG6583539.11.5e-30299.26Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG6583527.19.8e-30299.08Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023520298.11.6e-29998.53uncharacterized protein LOC111783614 [Cucurbita pepo subsp. pepo][more]
XP_023521328.17.8e-29998.34uncharacterized protein LOC111785084 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G28120.13.4e-14351.22Major facilitator superfamily protein [more]
AT2G39210.11.1e-13347.48Major facilitator superfamily protein [more]
AT5G50630.13.4e-7434.44Major facilitator superfamily protein [more]
AT5G50520.13.4e-7434.44Major facilitator superfamily protein [more]
AT5G14120.11.7e-7032.78Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 4..236
e-value: 1.7E-69
score: 234.1
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 298..538
e-value: 3.3E-12
score: 48.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 7..504
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 1..519
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 1..519

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G002140.1CmoCh13G002140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity